Rroxscaffold_2G00148040

Cytokinin dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013976
Physical Location & Seq
Reverse (-)
85700147 .. 85703146
3000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00148040.1

Sequence Viewer

Length: 1563 bp
ATGGCCAAGAATTCTTCTTCTTTCTCAACATACTTCATAGCCTTATTCTTCATGAGCCGCCTGATCCCCACCATTGGATACCGCCTCCGCGACGACCCCGAAGCCATTAGATTAGCTTCCACTGATTACGGCCACATCCACAGCGAAAACCCAGCCGCCGTTCTCTACCCTTCTTCCATAAACGACATTTCGTCCCTCATCAAGTTCGCCAACAATGTTTCCGTGCCGTTTGGCGTGGCCGCGAAAGGCCAGGGCCACTCCATACGGGGACAGGCCATGGCTCGCAATGGGGTCGTGGTCGAAATGGCGTCCCTGCGAAACCACCGAGCACAACACGGCTCCTCGGGAATCGAAGTAGTTTCAACCACCAACAAAGACGACGTGACGCTTAACTACGCGGACGTCGGAGGCGAGCAGCTCTGGGTCGATGTTCTGCATGCCACATTAGAACATGGACTTTCACCGGTGACGTGGACGGACTACCTTTACTTGACCGTAGGAGGGACGCTGTCGAACGCCGGAATCGGCGGACAGACGTTTCGGTTCGGCCCTCAGATCAGCAATGTCTACGAAATGGATGTTGTCACTGGAGAAGGGGATTTTGTGACTTGCTCCCCAAACAACAACGCAGAGCTTTTCTATGGGGTTCTTGGAGGGCTAGGTCAGTTTGGGGTTATAACCAGAGCCAGAATTGCACTAGAGCCAGCACCAAAAAGGGTCAAGTGGGTGCGAATGCTTTACAGTGACTTCTCTGCGTTTTCCAGAGACCAAGAACGTTTAATCTCAATCAATGGGCGACAACAAAGCAATGCCCTTGATTATTTGGAAGGCTCAATACTAATTAACCAGGGTCCCCCGGATAATTGGAGGGCTTCCTCATTTTTTCCTCAATCCTCTCACAACAGAATTATTTCCAAAGTAAACAAACATGGCATCATATACTGCCTCGAAGTGGTCAAATATTATGACCATCACACTGAAACCACTGCGGATAAGGAGCTCCAAAATCTGTTGAAAGGATTGAACTATCTTCCTGGATTTAAGTTTGAAAATGATGCGTCGTATGTCGAATTTCTGAATAGAGTTCGAAGCGGAGAGCTAAAGCTTCAGTCAGAAGGACTATGGGATGTCCCTCATCCGTGGCTTAATCTCTTTATTCCAAAAAGTCGCATCACGGACTTTGATCTTGGTGTGTTCAAGGACATTGTTCTAAAGAGAAATATTACCACCGGACCTGTCCTAGTTTACCCCATGAACAGGAGCAAGTGGAATGAGAGGATGTCGGCCGTTGTACCCGAAGAAGATGTGTTTTACACCGTAGGATTTTTGCATGCAACTAGGTTTGATGAATGGAAGGCATTTGATGAGCAAAATAAAGAGATATTAGGGTTTTGTGTTAGAGCTGGTATTGCTGTTAAGCAGTATCTTCCCGACCACAAAACACAGCACGGTTGGATGAATCATTTTGGCTCCAAATGGAAAACCTTCCAGAAGAGGAAAGCACTATTCGATCCCAAGAGAATACTTTCCCCTGGGCAAAGAATTTTTCATATTAAAGAATAA

Protein Analysis

520

Amino Acids

58.67

Weight (kDa)

8.49

Isoelectric Point (pI)

41.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 51 - 205 2.9e-14 FAD binding domain
Cytokin-bind PF09265 237 - 516 7e-117 Cytokinin dehydrogenase 1, FAD and cytokinin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56970 AT5G56970
fragaria_vesca FvH4_1g07620
malus_domestica MD15G1208300.v1.1
prunus_persica Prupe.7G208400_v2.0.a1 Prupe.7G208400_v2.0.a1
pyrus_communis pycom15g18450
rosa_chinensis RchiOBHm_Chr2g0093491
rosa_laevigata RLG00000016411
rosa_multiflora Rmu_sc0004423.1_g000008
rosa_roxburghii Rroxscaffold_2G00148040
rosa_rugosa Rorug02G0036700
rosa_samantha Rh2AG083200 Rh2BG083900 Rh2CG085900 Rh2DG082000
rosa_wichuraiana Rw2G006420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 677
AatII GACGTC 1 cut(s) 405
AccI GTMKAC 1 cut(s) 567
AccII CGCG 3 cut(s) 90, 242, 398
AciI CCGC 9 cut(s) 58, 82, 88, 156, 240, 398, 528, 989, 1092
AclI AACGTT 1 cut(s) 775
AclWI GGATC 2 cut(s) 58, 1505
AcoI YGGCCR 4 cut(s) 3, 130, 237, 1284
AcsI RAATTY 3 cut(s) 10, 1070, 1542
AcuI CTGAAG 1 cut(s) 1091
AcyI GRCGYC 2 cut(s) 308, 402
AfaI GTAC 1 cut(s) 1293
AfiI CCNNNNNNNGG 4 cut(s) 74, 501, 952, 1257
AgeI ACCGGT 1 cut(s) 463
AgsI TTSAA 5 cut(s) 363, 1015, 1024, 1049, 1198
AhdI GACNNNNNGTC 1 cut(s) 190
AjiI CACGTC 2 cut(s) 382, 471
AjnI CCWGG 4 cut(s) 249, 846, 1033, 1531
AluBI AGCT 7 cut(s) 116, 418, 634, 1000, 1099, 1105, 1403
AluI AGCT 7 cut(s) 116, 418, 634, 1000, 1099, 1105, 1403
Alw21I GWGCWC 2 cut(s) 331, 1002
Alw26I GTCTC 1 cut(s) 759
AlwI GGATC 2 cut(s) 58, 1505
Ama87I CYCGRG 1 cut(s) 343
AoxI GGCC 8 cut(s) 3, 130, 237, 247, 253, 273, 547, 1284
ApeKI GCWGC 1 cut(s) 415
ApoI RAATTY 3 cut(s) 10, 1070, 1542
ArsI GACNNNNNNTTYG 2 cut(s) 522, 554
AsiGI ACCGGT 1 cut(s) 463
Asp700I GAANNNNTTC 3 cut(s) 910, 1484, 1525
AspS9I GGNCC 4 cut(s) 253, 548, 851, 1232
AsuC2I CCSGG 1 cut(s) 857
AsuHPI GGTGA 2 cut(s) 453, 478
AsuII TTCGAA 1 cut(s) 1087
AvaI CYCGRG 1 cut(s) 343
AvaII GGWCC 2 cut(s) 851, 1232
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 1002
BarI GAAGNNNNNNTAC 2 cut(s) 819, 851
Bbv12I GWGCWC 2 cut(s) 331, 1002
BbvI GCAGC 1 cut(s) 427
BccI CCATC 1 cut(s) 978
BceAI ACGGC 5 cut(s) 143, 145, 211, 352, 1271
BcgI CGANNNNNNTGC 2 cut(s) 274, 308
BciT130I CCWGG 4 cut(s) 251, 848, 1035, 1533
BciVI GTATCC 1 cut(s) 71
BcnI CCSGG 1 cut(s) 857
BcoDI GTCTC 1 cut(s) 759
BfaI CTAG 4 cut(s) 659, 698, 1241, 1338
BfuI GTATCC 1 cut(s) 71
BisI GCNGC 4 cut(s) 58, 156, 240, 416
BlsI GCNGC 4 cut(s) 59, 157, 241, 417
Bme1390I CCNGG 5 cut(s) 251, 848, 857, 1035, 1533
Bme18I GGWCC 2 cut(s) 851, 1232
BmeRI GACNNNNNGTC 1 cut(s) 190
BmeT110I CYCGRG 1 cut(s) 343
BmgBI CACGTC 2 cut(s) 382, 471
BmgT120I GGNCC 4 cut(s) 253, 548, 851, 1232
BmiI GGNNCC 4 cut(s) 340, 852, 853, 1471
BmrFI CCNGG 5 cut(s) 251, 848, 857, 1035, 1533
BmsI GCATC 3 cut(s) 942, 1045, 1179
BpmI CTGGAG 1 cut(s) 609
Bpu14I TTCGAA 1 cut(s) 1087
BpuMI CCSGG 1 cut(s) 857
BsaHI GRCGYC 2 cut(s) 308, 402
BsaI GGTCTC 1 cut(s) 759
BsaJI CCNNGG 8 cut(s) 250, 276, 342, 847, 855, 1139, 1531, 1532
BsaWI WCCGGW 2 cut(s) 463, 1229
Bsc4I CCNNNNNNNGG 4 cut(s) 74, 501, 952, 1257
Bse118I RCCGGY 1 cut(s) 463
Bse1I ACTGG 1 cut(s) 592
Bse3DI GCAATG 3 cut(s) 292, 568, 814
BseBI CCWGG 4 cut(s) 251, 848, 1035, 1533
BseDI CCNNGG 8 cut(s) 250, 276, 342, 847, 855, 1139, 1531, 1532
BseGI GGATG 6 cut(s) 135, 583, 1132, 1135, 1284, 1461
BseLI CCNNNNNNNGG 4 cut(s) 74, 501, 952, 1257
BseMI GCAATG 3 cut(s) 292, 568, 814
BseMII CTCAG 1 cut(s) 566
BseNI ACTGG 1 cut(s) 592
BseRI GAGGAG 1 cut(s) 331
BseX3I CGGCCG 1 cut(s) 1284
BseXI GCAGC 1 cut(s) 427
BseYI CCCAGC 1 cut(s) 151
Bsh1236I CGCG 3 cut(s) 90, 242, 398
Bsh1285I CGRYCG 1 cut(s) 1287
BshFI GGCC 8 cut(s) 5, 132, 239, 249, 255, 275, 549, 1286
BshTI ACCGGT 1 cut(s) 463
BsiEI CGRYCG 1 cut(s) 1287
BsiHKAI GWGCWC 2 cut(s) 331, 1002
BsiHKCI CYCGRG 1 cut(s) 343
BsiSI CCGG 4 cut(s) 464, 519, 857, 1230
BslFI GGGAC 6 cut(s) 178, 282, 295, 517, 837, 1115
BslI CCNNNNNNNGG 4 cut(s) 74, 501, 952, 1257
BsmAI GTCTC 1 cut(s) 759
BsmFI GGGAC 6 cut(s) 178, 282, 295, 517, 837, 1115
BsmI GAATGC 1 cut(s) 738
BsnI GGCC 8 cut(s) 5, 132, 239, 249, 255, 275, 549, 1286
Bso31I GGTCTC 1 cut(s) 759
BsoBI CYCGRG 1 cut(s) 343
Bsp119I TTCGAA 1 cut(s) 1087
Bsp1286I GDGCHC 2 cut(s) 331, 1002
Bsp143I GATC 4 cut(s) 63, 555, 1183, 1510
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 9 cut(s) 58, 82, 88, 156, 240, 398, 528, 989, 1092
BspANI GGCC 8 cut(s) 5, 132, 239, 249, 255, 275, 549, 1286
BspCNI CTCAG 1 cut(s) 565
BspFNI CGCG 3 cut(s) 90, 242, 398
BspHI TCATGA 1 cut(s) 51
BspLI GGNNCC 4 cut(s) 340, 852, 853, 1471
BspPI GGATC 2 cut(s) 58, 1505
BspT104I TTCGAA 1 cut(s) 1087
BspTNI GGTCTC 1 cut(s) 759
BsrDI GCAATG 3 cut(s) 292, 568, 814
BsrFI RCCGGY 1 cut(s) 463
BsrI ACTGG 1 cut(s) 592
BssAI RCCGGY 1 cut(s) 463
BssECI CCNNGG 8 cut(s) 250, 276, 342, 847, 855, 1139, 1531, 1532
BssMI GATC 4 cut(s) 63, 555, 1183, 1510
BssNI GRCGYC 2 cut(s) 308, 402
BssT1I CCWWGG 1 cut(s) 276
Bst2UI CCWGG 4 cut(s) 251, 848, 1035, 1533
Bst4CI ACNGT 4 cut(s) 496, 743, 1318, 1451
Bst6I CTCTTC 1 cut(s) 1487
BstACI GRCGYC 2 cut(s) 308, 402
BstBI TTCGAA 1 cut(s) 1087
BstC8I GCNNGC 5 cut(s) 283, 413, 438, 705, 1332
BstDEI CTNAG 1 cut(s) 552
BstDSI CCRYGG 2 cut(s) 276, 1139
BstF5I GGATG 6 cut(s) 135, 583, 1132, 1135, 1284, 1461
BstFNI CGCG 3 cut(s) 90, 242, 398
BstKTI GATC 4 cut(s) 66, 558, 1186, 1513
BstMAI GTCTC 1 cut(s) 759
BstMBI GATC 4 cut(s) 63, 555, 1183, 1510
BstMCI CGRYCG 1 cut(s) 1287
BstMWI GCNNNNNNNGC 2 cut(s) 692, 1409
BstNI CCWGG 4 cut(s) 251, 848, 1035, 1533
BstNSI RCATGY 2 cut(s) 440, 1334
BstSCI CCNGG 5 cut(s) 249, 846, 855, 1033, 1531
BstUI CGCG 3 cut(s) 90, 242, 398
BstV1I GCAGC 1 cut(s) 427
BstZI CGGCCG 1 cut(s) 1284
BsuI GTATCC 1 cut(s) 71
BsuRI GGCC 8 cut(s) 5, 132, 239, 249, 255, 275, 549, 1286
BtgI CCRYGG 2 cut(s) 276, 1139
BtrI CACGTC 2 cut(s) 382, 471
BtsCI GGATG 6 cut(s) 135, 583, 1132, 1135, 1284, 1461
BtsI GCAGTG 1 cut(s) 984
BtsIMutI CAGTG 5 cut(s) 120, 585, 748, 975, 984
Cac8I GCNNGC 5 cut(s) 283, 413, 438, 705, 1332
CciI TCATGA 1 cut(s) 51
Cfr10I RCCGGY 1 cut(s) 463
Cfr13I GGNCC 4 cut(s) 253, 548, 851, 1232
CseI GACGC 4 cut(s) 297, 394, 514, 1047
Csp6I GTAC 1 cut(s) 1292
CspAI ACCGGT 1 cut(s) 463
CviAII CATG 7 cut(s) 52, 277, 437, 452, 929, 1252, 1331
CviQI GTAC 1 cut(s) 1292
DdeI CTNAG 1 cut(s) 552
DpnI GATC 4 cut(s) 65, 557, 1185, 1512
DpnII GATC 4 cut(s) 63, 555, 1183, 1510
DriI GACNNNNNGTC 1 cut(s) 190
EaeI YGGCCR 4 cut(s) 3, 130, 237, 1284
EagI CGGCCG 1 cut(s) 1284
Eam1104I CTCTTC 1 cut(s) 1487
Eam1105I GACNNNNNGTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 1487
EciI GGCGGA 1 cut(s) 543
Ecl136II GAGCTC 1 cut(s) 1000
EclXI CGGCCG 1 cut(s) 1284
Eco130I CCWWGG 1 cut(s) 276
Eco24I GRGCYC 1 cut(s) 1002
Eco31I GGTCTC 1 cut(s) 759
Eco47I GGWCC 2 cut(s) 851, 1232
Eco52I CGGCCG 1 cut(s) 1284
Eco53kI GAGCTC 1 cut(s) 1000
Eco57I CTGAAG 1 cut(s) 1091
Eco88I CYCGRG 1 cut(s) 343
EcoICRI GAGCTC 1 cut(s) 1000
EcoO109I RGGNCCY 1 cut(s) 851
EcoRI GAATTC 1 cut(s) 10
EcoRII CCWGG 4 cut(s) 249, 846, 1033, 1531
EcoT14I CCWWGG 1 cut(s) 276
EcoT38I GRGCYC 1 cut(s) 1002
ErhI CCWWGG 1 cut(s) 276
FaeI CATG 7 cut(s) 55, 280, 440, 455, 932, 1255, 1334
FaqI GGGAC 6 cut(s) 178, 282, 295, 517, 837, 1115
FatI CATG 7 cut(s) 51, 276, 436, 451, 928, 1251, 1330
FblI GTMKAC 1 cut(s) 567
Fnu4HI GCNGC 4 cut(s) 58, 156, 240, 416
FokI GGATG 6 cut(s) 122, 590, 1122, 1139, 1291, 1468
FriOI GRGCYC 1 cut(s) 1002
Fsp4HI GCNGC 4 cut(s) 58, 156, 240, 416
FspBI CTAG 4 cut(s) 659, 698, 1241, 1338
GluI GCNGC 4 cut(s) 58, 156, 240, 416
GsaI CCCAGC 1 cut(s) 155
GsuI CTGGAG 1 cut(s) 609
HaeIII GGCC 8 cut(s) 5, 132, 239, 249, 255, 275, 549, 1286
HapII CCGG 4 cut(s) 464, 519, 857, 1230
HgaI GACGC 4 cut(s) 297, 394, 514, 1047
Hin1I GRCGYC 2 cut(s) 308, 402
Hin1II CATG 7 cut(s) 55, 280, 440, 455, 932, 1255, 1334
HindIII AAGCTT 1 cut(s) 1103
HinfI GANTC 3 cut(s) 348, 522, 1459
HpaII CCGG 4 cut(s) 464, 519, 857, 1230
HphI GGTGA 2 cut(s) 453, 478
Hpy166II GTNNAC 4 cut(s) 474, 568, 922, 1246
Hpy188I TCNGA 4 cut(s) 407, 555, 1077, 1114
Hpy188III TCNNGA 5 cut(s) 52, 345, 762, 1430, 1489
Hpy8I GTNNAC 4 cut(s) 474, 568, 922, 1246
Hpy99I CGWCG 4 cut(s) 95, 383, 407, 1063
HpyAV CCTTC 6 cut(s) 180, 587, 821, 1109, 1348, 1495
HpyCH4III ACNGT 4 cut(s) 496, 743, 1318, 1451
HpyCH4IV ACGT 5 cut(s) 381, 402, 470, 536, 775
HpyCH4V TGCA 4 cut(s) 436, 695, 1330, 1334
HpyF10VI GCNNNNNNNGC 2 cut(s) 692, 1409
HpyF3I CTNAG 1 cut(s) 552
HpySE526I ACGT 5 cut(s) 381, 402, 470, 536, 775
Hsp92I GRCGYC 2 cut(s) 308, 402
Hsp92II CATG 7 cut(s) 55, 280, 440, 455, 932, 1255, 1334
KflI GGGWCCC 1 cut(s) 851
Kzo9I GATC 4 cut(s) 63, 555, 1183, 1510
LmnI GCTCC 6 cut(s) 344, 617, 997, 1005, 1260, 1475
Lsp1109I GCAGC 1 cut(s) 427
LweI GCATC 3 cut(s) 942, 1045, 1179
MaeI CTAG 4 cut(s) 659, 698, 1241, 1338
MaeII ACGT 5 cut(s) 381, 402, 470, 536, 775
MaeIII GTNAC 5 cut(s) 382, 466, 583, 604, 743
MalI GATC 4 cut(s) 65, 557, 1185, 1512
MboI GATC 4 cut(s) 63, 555, 1183, 1510
MboII GAAGA 9 cut(s) 6, 9, 40, 165, 1022, 1310, 1313, 1418, 1504
MhlI GDGCHC 2 cut(s) 331, 1002
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 10, 690, 840, 862, 906, 1070, 1542
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 385, 1433
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 3 cut(s) 910, 1484, 1525
MscI TGGCCA 1 cut(s) 5
MseI TTAA 7 cut(s) 390, 779, 843, 1041, 1146, 1416, 1554
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 4 cut(s) 464, 519, 857, 1230
MspR9I CCNGG 5 cut(s) 251, 848, 857, 1035, 1533
Mva1269I GAATGC 1 cut(s) 738
MvaI CCWGG 4 cut(s) 251, 848, 1035, 1533
MvnI CGCG 3 cut(s) 90, 242, 398
MwoI GCNNNNNNNGC 2 cut(s) 692, 1409
NciI CCSGG 1 cut(s) 857
NcoI CCATGG 1 cut(s) 276
NdeII GATC 4 cut(s) 63, 555, 1183, 1510
NlaIII CATG 7 cut(s) 55, 280, 440, 455, 932, 1255, 1334
NlaIV GGNNCC 4 cut(s) 340, 852, 853, 1471
NmuCI GTSAC 5 cut(s) 382, 466, 583, 604, 743
NspI RCATGY 2 cut(s) 440, 1334
NspV TTCGAA 1 cut(s) 1087
PaeI GCATGC 2 cut(s) 440, 1334
PagI TCATGA 1 cut(s) 51
PasI CCCWGGG 1 cut(s) 1532
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PctI GAATGC 1 cut(s) 738
PdmI GAANNNNTTC 3 cut(s) 910, 1484, 1525
PfeI GAWTC 3 cut(s) 348, 522, 1459
PflFI GACNNNGTC 1 cut(s) 508
PfoI TCCNGGA 1 cut(s) 1033
PinAI ACCGGT 1 cut(s) 463
PkrI GCNGC 4 cut(s) 59, 157, 241, 417
PpuMI RGGWCCY 1 cut(s) 851
PsiI TTATAA 1 cut(s) 677
Psp124BI GAGCTC 1 cut(s) 1002
Psp1406I AACGTT 1 cut(s) 775
Psp5II RGGWCCY 1 cut(s) 851
Psp6I CCWGG 4 cut(s) 249, 846, 1033, 1531
PspFI CCCAGC 1 cut(s) 151
PspGI CCWGG 4 cut(s) 249, 846, 1033, 1531
PspN4I GGNNCC 4 cut(s) 340, 852, 853, 1471
PspPI GGNCC 4 cut(s) 253, 548, 851, 1232
PspPPI RGGWCCY 1 cut(s) 851
PsyI GACNNNGTC 1 cut(s) 508
RsaI GTAC 1 cut(s) 1293
RsaNI GTAC 1 cut(s) 1292
SacI GAGCTC 1 cut(s) 1002
SaqAI TTAA 7 cut(s) 390, 779, 843, 1041, 1146, 1416, 1554
SatI GCNGC 4 cut(s) 58, 156, 240, 416
Sau3AI GATC 4 cut(s) 63, 555, 1183, 1510
Sau96I GGNCC 4 cut(s) 253, 548, 851, 1232
ScrFI CCNGG 5 cut(s) 251, 848, 857, 1035, 1533
SduI GDGCHC 2 cut(s) 331, 1002
SfaNI GCATC 3 cut(s) 942, 1045, 1179
SfuI TTCGAA 1 cut(s) 1087
SgrAI CRCCGGYG 1 cut(s) 463
SinI GGWCC 2 cut(s) 851, 1232
SphI GCATGC 2 cut(s) 440, 1334
Sse9I AATT 7 cut(s) 10, 690, 840, 862, 906, 1070, 1542
SsiI CCGC 9 cut(s) 58, 82, 88, 156, 240, 398, 528, 989, 1092
SspI AATATT 2 cut(s) 962, 1222
SspMI CTAG 4 cut(s) 659, 698, 1241, 1338
SstI GAGCTC 1 cut(s) 1002
StyD4I CCNGG 5 cut(s) 249, 846, 855, 1033, 1531
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 4 cut(s) 496, 743, 1318, 1451
TaiI ACGT 5 cut(s) 384, 405, 473, 539, 778
TaqI TCGA 8 cut(s) 300, 351, 426, 512, 948, 1068, 1087, 1509
TasI AATT 7 cut(s) 10, 690, 840, 862, 906, 1070, 1542
TauI GCSGC 3 cut(s) 60, 158, 242
TfiI GAWTC 3 cut(s) 348, 522, 1459
Tru1I TTAA 7 cut(s) 390, 779, 843, 1041, 1146, 1416, 1554
Tru9I TTAA 7 cut(s) 390, 779, 843, 1041, 1146, 1416, 1554
TscAI CASTG 5 cut(s) 127, 592, 748, 982, 991
TseFI GTSAC 5 cut(s) 382, 466, 583, 604, 743
TseI GCWGC 1 cut(s) 415
Tsp45I GTSAC 5 cut(s) 382, 466, 583, 604, 743
TspDTI ATGAA 6 cut(s) 25, 40, 1268, 1362, 1472, 1538
TspGWI ACGGA 4 cut(s) 211, 491, 1128, 1190
TspRI CASTG 5 cut(s) 127, 592, 748, 982, 991
Tth111I GACNNNGTC 1 cut(s) 508
VpaK11BI GGWCC 2 cut(s) 851, 1232
XapI RAATTY 3 cut(s) 10, 1070, 1542
XceI RCATGY 2 cut(s) 440, 1334
XmiI GTMKAC 1 cut(s) 567
XmnI GAANNNNTTC 3 cut(s) 910, 1484, 1525
XspI CTAG 4 cut(s) 659, 698, 1241, 1338
ZraI GACGTC 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.