RchiOBHm_Chr2g0093491

Cytokinin dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0013976
Physical Location & Seq
Forward (+)
6765430 .. 6768683
3254 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46854

Sequence Viewer

Length: 1833 bp
ATGACGTTTGCATCAGAAAGAAAAAGAGAGAATGATGAATCCAAGGCCTGTCCTTTGCGTATCTTGGAAAACCAATACGTGTTGAGCTTACGACGTCGTTCCCCACACAATGCCATACACATTTTTAAACGCCAAAGATTTCGTTCTCTGCCAATACAAGATTTTTACCCTCGTTCTCATGCAGCTTTATTTCCCTATATATACCTCTCTTCATCCTTTTATATCCTACCTACCTACCCATTCCTTCTCTGCCTCACTTTCTCATTTTCAATGGCCAAGAATTCTTCTTCTTTCCCAACATACTTCATAGCCTTATTCATCATGAGCCGCCTGATCCCCACCATTGGATACCGCCTCCGCGACGACCCCGAAGCCATTATATTAGCTTCCACTGATTACGGCCACATCCACAGCGAAAACCCAGCCGCCGTTCTCTACCCTTCTTCCATAAACGACATTTCGTCCCTCATCAAGTTCGCCAACAATGTTTCCGTGCCGTTTGGCGTGGCTGCGAAAGGCCAGGGCCACTCGACACGGGGACAGGCCATGGCTCGCAATGGGGTCGTGGTCGAAATGTCGTCCCTGCGAAACCACCGACCACAACACGGCTCCTCCGGAATCGAAGTAGTTTCAACCACCAACAAAGACGAAGTGAAGCTTTACTACGCGGACGTCGGAGGCGAGCAGCTCTGGGTCGATGTTCTGCATGCCACATTAGAACATGGACTTTCACCGGTGACGTGGACGGACTACCTTTACTTGACCGTAGGAGGGACGCTGTCGAACGCCGGAATCGGCGGACAGACGTTTCGGTTCGGCCCTCAGATCAGCAATGTCTACGAAATGGATGTTGTCACTGGAGAAGGGGATTTTGTGACTTGCTCCCCAAACAACAACGCAGAGCTTTTCTATGGGGTTCTTGGAGGGCTAGGTCAGTTTGGGGTTATATCCAGAGCCAGAATTGCACTAGAGCCAGCACCAAAAAGGGTCAAGTGGGTGCGAATGCTTTACAGTGACTTCTCTGCGTTTTCCAGAGACCAAGAACGTTTAATCTCAATCAATGGGCGACAACAAAGCAATGCCCTTGATTATTTGGAAGGCTCAATACTAATTAACCAGGGTCCCCCGGATAATTGGAGGGCTTCCTCATTTTTTCCTCAATCCTCTCACAACAGAATTATTTCCAAAGTAAACAAACATGGGATCATATACTGCCTCGAAGTGGTCAAATATTATGACCATCACACTGAAACCACTGCGGATAAGGAGCTCCAAAATCTATTGAAAGGATTGAACTATCTTCCTGGATTTAAGTTTGAAAATGATGCGTCGTATGTCGAATTTCTGAATAGAGTTCGAAGCGGAGAGCTAAAGCTTCAGTCAGAAGGACTATGGGATGTCCCTCATCCGTGGCTTAATCTCTTTATTCCAAAAAGTCGCATCACGGACTTTGATCTTGGTGTGTTCAAGGACATTGTTCTGAAGAGAAATATTACCACCGGACCTGTCCTAGTTTACCCCATGAACAGGAGCAAGTGGAATGAGAGGATGTCGGCCGTTGTACCCGAAGAAGATGTGTTTTACACCGTAGGATTTTTGCATTCAACTGGGTTTGATGAATGGAAGGCATTTGATGAGCAAAATAAAGAGATATTAGGGTTTTGTGTTAGATCTGGTATTGCTGTTAAGCAGTATCTTCCCGACCACAAAACACAGCAAGATTGGATGAATCATTTTGGCTCCAAATGGAAAACCTTCCAGAAGAGGAAAGCACTATTCGATCCCAAGAGAATACTTTCCCCTGGGCAAAGAATTTTTCAGATTAAAGAATAA

Protein Analysis

610

Amino Acids

69.47

Weight (kDa)

8.95

Isoelectric Point (pI)

54.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 141 - 295 5.6e-14 FAD binding domain
Cytokin-bind PF09265 327 - 606 2.2e-117 Cytokinin dehydrogenase 1, FAD and cytokinin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56970 AT5G56970
fragaria_vesca FvH4_1g07620
malus_domestica MD15G1208300.v1.1
prunus_persica Prupe.7G208400_v2.0.a1 Prupe.7G208400_v2.0.a1
pyrus_communis pycom15g18450
rosa_chinensis RchiOBHm_Chr2g0093491
rosa_laevigata RLG00000016411
rosa_multiflora Rmu_sc0004423.1_g000008
rosa_roxburghii Rroxscaffold_2G00148040
rosa_rugosa Rorug02G0036700
rosa_samantha Rh2AG083200 Rh2BG083900 Rh2CG085900 Rh2DG082000
rosa_wichuraiana Rw2G006420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 97, 675
AccI GTMKAC 1 cut(s) 837
AccII CGCG 2 cut(s) 360, 668
AccIII TCCGGA 1 cut(s) 614
AciI CCGC 8 cut(s) 328, 352, 358, 426, 668, 798, 1259, 1362
AclI AACGTT 1 cut(s) 1045
AclWI GGATC 3 cut(s) 328, 1211, 1775
AcoI YGGCCR 3 cut(s) 273, 400, 1554
AcsI RAATTY 3 cut(s) 280, 1340, 1812
AcuI CTGAAG 2 cut(s) 1361, 1502
AcyI GRCGYC 2 cut(s) 94, 672
AfaI GTAC 1 cut(s) 1563
AfiI CCNNNNNNNGG 5 cut(s) 344, 605, 771, 1222, 1527
AflIII ACRYGT 1 cut(s) 78
AgeI ACCGGT 1 cut(s) 733
AgsI TTSAA 7 cut(s) 270, 633, 1285, 1294, 1319, 1468, 1605
AhdI GACNNNNNGTC 1 cut(s) 460
AjiI CACGTC 1 cut(s) 741
AjnI CCWGG 4 cut(s) 519, 1116, 1303, 1801
AluBI AGCT 9 cut(s) 87, 185, 386, 658, 688, 904, 1270, 1369, 1375
AluI AGCT 9 cut(s) 87, 185, 386, 658, 688, 904, 1270, 1369, 1375
Alw21I GWGCWC 1 cut(s) 1272
Alw26I GTCTC 1 cut(s) 1029
AlwI GGATC 3 cut(s) 328, 1211, 1775
Aor13HI TCCGGA 1 cut(s) 614
AoxI GGCC 8 cut(s) 45, 273, 400, 517, 523, 543, 817, 1554
ApeKI GCWGC 3 cut(s) 182, 509, 685
ApoI RAATTY 3 cut(s) 280, 1340, 1812
ArsI GACNNNNNNTTYG 2 cut(s) 792, 824
AsiGI ACCGGT 1 cut(s) 733
Asp700I GAANNNNTTC 3 cut(s) 1180, 1754, 1795
AspS9I GGNCC 4 cut(s) 523, 818, 1121, 1502
AsuC2I CCSGG 1 cut(s) 1127
AsuHPI GGTGA 2 cut(s) 723, 748
AsuII TTCGAA 1 cut(s) 1357
AvaII GGWCC 2 cut(s) 1121, 1502
BalI TGGCCA 1 cut(s) 275
BanII GRGCYC 1 cut(s) 1272
BarI GAAGNNNNNNTAC 4 cut(s) 647, 679, 1089, 1121
Bbv12I GWGCWC 1 cut(s) 1272
BbvI GCAGC 3 cut(s) 194, 496, 697
BccI CCATC 1 cut(s) 1248
BceAI ACGGC 5 cut(s) 413, 415, 481, 622, 1541
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 4 cut(s) 521, 1118, 1305, 1803
BciVI GTATCC 1 cut(s) 341
BcnI CCSGG 1 cut(s) 1127
BcoDI GTCTC 1 cut(s) 1029
BfaI CTAG 3 cut(s) 929, 968, 1511
BfuI GTATCC 1 cut(s) 341
BglII AGATCT 1 cut(s) 1670
BisI GCNGC 5 cut(s) 183, 328, 426, 510, 686
BlsI GCNGC 5 cut(s) 184, 329, 427, 511, 687
Bme1390I CCNGG 5 cut(s) 521, 1118, 1127, 1305, 1803
Bme18I GGWCC 2 cut(s) 1121, 1502
BmeRI GACNNNNNGTC 1 cut(s) 460
BmgBI CACGTC 1 cut(s) 741
BmgT120I GGNCC 4 cut(s) 523, 818, 1121, 1502
BmiI GGNNCC 4 cut(s) 610, 1122, 1123, 1741
BmrFI CCNGG 5 cut(s) 521, 1118, 1127, 1305, 1803
BmrI ACTGGG 1 cut(s) 1617
BmsI GCATC 3 cut(s) 20, 1315, 1449
BmuI ACTGGG 1 cut(s) 1617
BpmI CTGGAG 1 cut(s) 879
Bpu14I TTCGAA 1 cut(s) 1357
BpuMI CCSGG 1 cut(s) 1127
BsaAI YACGTR 1 cut(s) 79
BsaHI GRCGYC 2 cut(s) 94, 672
BsaI GGTCTC 1 cut(s) 1029
BsaJI CCNNGG 8 cut(s) 42, 520, 546, 1117, 1125, 1409, 1801, 1802
BsaWI WCCGGW 3 cut(s) 614, 733, 1499
BsaXI ACNNNNNCTCC 2 cut(s) 596, 626
Bsc4I CCNNNNNNNGG 5 cut(s) 344, 605, 771, 1222, 1527
Bse118I RCCGGY 1 cut(s) 733
Bse1I ACTGG 2 cut(s) 862, 1612
Bse3DI GCAATG 3 cut(s) 562, 838, 1084
BseAI TCCGGA 1 cut(s) 614
BseBI CCWGG 4 cut(s) 521, 1118, 1305, 1803
BseDI CCNNGG 8 cut(s) 42, 520, 546, 1117, 1125, 1409, 1801, 1802
BseGI GGATG 7 cut(s) 212, 405, 853, 1402, 1405, 1554, 1731
BseLI CCNNNNNNNGG 5 cut(s) 344, 605, 771, 1222, 1527
BseMI GCAATG 3 cut(s) 562, 838, 1084
BseMII CTCAG 1 cut(s) 836
BseNI ACTGG 2 cut(s) 862, 1612
BseRI GAGGAG 1 cut(s) 601
BseX3I CGGCCG 1 cut(s) 1554
BseXI GCAGC 3 cut(s) 194, 496, 697
BseYI CCCAGC 1 cut(s) 421
Bsh1236I CGCG 2 cut(s) 360, 668
Bsh1285I CGRYCG 1 cut(s) 1557
BshFI GGCC 8 cut(s) 47, 275, 402, 519, 525, 545, 819, 1556
BshTI ACCGGT 1 cut(s) 733
BsiEI CGRYCG 1 cut(s) 1557
BsiHKAI GWGCWC 1 cut(s) 1272
BsiSI CCGG 5 cut(s) 615, 734, 789, 1127, 1500
BslFI GGGAC 6 cut(s) 448, 552, 565, 787, 1107, 1385
BslI CCNNNNNNNGG 5 cut(s) 344, 605, 771, 1222, 1527
BsmAI GTCTC 1 cut(s) 1029
BsmFI GGGAC 6 cut(s) 448, 552, 565, 787, 1107, 1385
BsmI GAATGC 2 cut(s) 1008, 1600
BsnI GGCC 8 cut(s) 47, 275, 402, 519, 525, 545, 819, 1556
Bso31I GGTCTC 1 cut(s) 1029
Bsp119I TTCGAA 1 cut(s) 1357
Bsp1286I GDGCHC 1 cut(s) 1272
Bsp13I TCCGGA 1 cut(s) 614
Bsp143I GATC 6 cut(s) 333, 825, 1203, 1453, 1670, 1780
Bsp19I CCATGG 1 cut(s) 546
BspACI CCGC 8 cut(s) 328, 352, 358, 426, 668, 798, 1259, 1362
BspANI GGCC 8 cut(s) 47, 275, 402, 519, 525, 545, 819, 1556
BspCNI CTCAG 1 cut(s) 835
BspEI TCCGGA 1 cut(s) 614
BspFNI CGCG 2 cut(s) 360, 668
BspHI TCATGA 1 cut(s) 321
BspLI GGNNCC 4 cut(s) 610, 1122, 1123, 1741
BspPI GGATC 3 cut(s) 328, 1211, 1775
BspT104I TTCGAA 1 cut(s) 1357
BspTNI GGTCTC 1 cut(s) 1029
BsrDI GCAATG 3 cut(s) 562, 838, 1084
BsrFI RCCGGY 1 cut(s) 733
BsrI ACTGG 2 cut(s) 862, 1612
BssAI RCCGGY 1 cut(s) 733
BssECI CCNNGG 8 cut(s) 42, 520, 546, 1117, 1125, 1409, 1801, 1802
BssMI GATC 6 cut(s) 333, 825, 1203, 1453, 1670, 1780
BssNI GRCGYC 2 cut(s) 94, 672
BssT1I CCWWGG 2 cut(s) 42, 546
Bst2UI CCWGG 4 cut(s) 521, 1118, 1305, 1803
Bst4CI ACNGT 3 cut(s) 766, 1013, 1588
Bst6I CTCTTC 3 cut(s) 214, 1478, 1757
BstACI GRCGYC 2 cut(s) 94, 672
BstBAI YACGTR 1 cut(s) 79
BstBI TTCGAA 1 cut(s) 1357
BstC8I GCNNGC 4 cut(s) 553, 683, 708, 975
BstDEI CTNAG 1 cut(s) 822
BstDSI CCRYGG 2 cut(s) 546, 1409
BstF5I GGATG 7 cut(s) 212, 405, 853, 1402, 1405, 1554, 1731
BstFNI CGCG 2 cut(s) 360, 668
BstKTI GATC 6 cut(s) 336, 828, 1206, 1456, 1673, 1783
BstMAI GTCTC 1 cut(s) 1029
BstMBI GATC 6 cut(s) 333, 825, 1203, 1453, 1670, 1780
BstMCI CGRYCG 1 cut(s) 1557
BstMWI GCNNNNNNNGC 1 cut(s) 962
BstNI CCWGG 4 cut(s) 521, 1118, 1305, 1803
BstNSI RCATGY 1 cut(s) 710
BstSCI CCNGG 5 cut(s) 519, 1116, 1125, 1303, 1801
BstUI CGCG 2 cut(s) 360, 668
BstV1I GCAGC 3 cut(s) 194, 496, 697
BstX2I RGATCY 1 cut(s) 1670
BstYI RGATCY 1 cut(s) 1670
BstZI CGGCCG 1 cut(s) 1554
BsuI GTATCC 1 cut(s) 341
BsuRI GGCC 8 cut(s) 47, 275, 402, 519, 525, 545, 819, 1556
BtgI CCRYGG 2 cut(s) 546, 1409
BtrI CACGTC 1 cut(s) 741
BtsCI GGATG 7 cut(s) 212, 405, 853, 1402, 1405, 1554, 1731
BtsI GCAGTG 1 cut(s) 1254
BtsIMutI CAGTG 5 cut(s) 390, 855, 1018, 1245, 1254
Cac8I GCNNGC 4 cut(s) 553, 683, 708, 975
CciI TCATGA 1 cut(s) 321
Cfr10I RCCGGY 1 cut(s) 733
Cfr13I GGNCC 4 cut(s) 523, 818, 1121, 1502
CseI GACGC 2 cut(s) 784, 1317
Csp6I GTAC 1 cut(s) 1562
CspAI ACCGGT 1 cut(s) 733
CviAII CATG 7 cut(s) 179, 322, 547, 707, 722, 1199, 1522
CviQI GTAC 1 cut(s) 1562
DdeI CTNAG 1 cut(s) 822
DpnI GATC 6 cut(s) 335, 827, 1205, 1455, 1672, 1782
DpnII GATC 6 cut(s) 333, 825, 1203, 1453, 1670, 1780
DraI TTTAAA 1 cut(s) 127
DriI GACNNNNNGTC 1 cut(s) 460
EaeI YGGCCR 3 cut(s) 273, 400, 1554
EagI CGGCCG 1 cut(s) 1554
Eam1104I CTCTTC 3 cut(s) 214, 1478, 1757
Eam1105I GACNNNNNGTC 1 cut(s) 460
EarI CTCTTC 3 cut(s) 214, 1478, 1757
EciI GGCGGA 1 cut(s) 813
Ecl136II GAGCTC 1 cut(s) 1270
EclXI CGGCCG 1 cut(s) 1554
Eco130I CCWWGG 2 cut(s) 42, 546
Eco147I AGGCCT 1 cut(s) 47
Eco24I GRGCYC 1 cut(s) 1272
Eco31I GGTCTC 1 cut(s) 1029
Eco47I GGWCC 2 cut(s) 1121, 1502
Eco52I CGGCCG 1 cut(s) 1554
Eco53kI GAGCTC 1 cut(s) 1270
Eco57I CTGAAG 2 cut(s) 1361, 1502
EcoICRI GAGCTC 1 cut(s) 1270
EcoO109I RGGNCCY 1 cut(s) 1121
EcoRI GAATTC 1 cut(s) 280
EcoRII CCWGG 4 cut(s) 519, 1116, 1303, 1801
EcoT14I CCWWGG 2 cut(s) 42, 546
EcoT38I GRGCYC 1 cut(s) 1272
ErhI CCWWGG 2 cut(s) 42, 546
FaeI CATG 7 cut(s) 182, 325, 550, 710, 725, 1202, 1525
FalI AAGNNNNNCTT 2 cut(s) 642, 674
FaqI GGGAC 6 cut(s) 448, 552, 565, 787, 1107, 1385
FatI CATG 7 cut(s) 178, 321, 546, 706, 721, 1198, 1521
FblI GTMKAC 1 cut(s) 837
Fnu4HI GCNGC 5 cut(s) 183, 328, 426, 510, 686
FokI GGATG 7 cut(s) 199, 392, 860, 1392, 1409, 1561, 1738
FriOI GRGCYC 1 cut(s) 1272
Fsp4HI GCNGC 5 cut(s) 183, 328, 426, 510, 686
FspBI CTAG 3 cut(s) 929, 968, 1511
GluI GCNGC 5 cut(s) 183, 328, 426, 510, 686
GsaI CCCAGC 1 cut(s) 425
GsuI CTGGAG 1 cut(s) 879
HaeIII GGCC 8 cut(s) 47, 275, 402, 519, 525, 545, 819, 1556
HapII CCGG 5 cut(s) 615, 734, 789, 1127, 1500
HgaI GACGC 2 cut(s) 784, 1317
Hin1I GRCGYC 2 cut(s) 94, 672
Hin1II CATG 7 cut(s) 182, 325, 550, 710, 725, 1202, 1525
HindIII AAGCTT 2 cut(s) 656, 1373
HinfI GANTC 4 cut(s) 38, 618, 792, 1729
HpaII CCGG 5 cut(s) 615, 734, 789, 1127, 1500
HphI GGTGA 2 cut(s) 723, 748
Hpy166II GTNNAC 4 cut(s) 744, 838, 1192, 1516
Hpy188I TCNGA 7 cut(s) 16, 677, 825, 1347, 1384, 1482, 1821
Hpy188III TCNNGA 6 cut(s) 322, 615, 951, 1032, 1700, 1759
Hpy8I GTNNAC 4 cut(s) 744, 838, 1192, 1516
Hpy99I CGWCG 5 cut(s) 96, 99, 365, 677, 1333
HpyAV CCTTC 7 cut(s) 254, 450, 857, 1091, 1379, 1618, 1765
HpyCH4III ACNGT 3 cut(s) 766, 1013, 1588
HpyCH4IV ACGT 7 cut(s) 5, 78, 94, 672, 740, 806, 1045
HpyCH4V TGCA 5 cut(s) 11, 182, 706, 965, 1600
HpyF10VI GCNNNNNNNGC 1 cut(s) 962
HpyF3I CTNAG 1 cut(s) 822
HpySE526I ACGT 7 cut(s) 5, 78, 94, 672, 740, 806, 1045
Hsp92I GRCGYC 2 cut(s) 94, 672
Hsp92II CATG 7 cut(s) 182, 325, 550, 710, 725, 1202, 1525
KflI GGGWCCC 1 cut(s) 1121
Kpn2I TCCGGA 1 cut(s) 614
Kzo9I GATC 6 cut(s) 333, 825, 1203, 1453, 1670, 1780
LmnI GCTCC 6 cut(s) 614, 887, 1267, 1275, 1530, 1745
Lsp1109I GCAGC 3 cut(s) 194, 496, 697
LweI GCATC 3 cut(s) 20, 1315, 1449
MaeI CTAG 3 cut(s) 929, 968, 1511
MaeII ACGT 7 cut(s) 5, 78, 94, 672, 740, 806, 1045
MaeIII GTNAC 4 cut(s) 736, 853, 874, 1013
MalI GATC 6 cut(s) 335, 827, 1205, 1455, 1672, 1782
MboI GATC 6 cut(s) 333, 825, 1203, 1453, 1670, 1780
MflI RGATCY 1 cut(s) 1670
MhlI GDGCHC 1 cut(s) 1272
MlsI TGGCCA 1 cut(s) 275
MluCI AATT 7 cut(s) 280, 960, 1110, 1132, 1176, 1340, 1812
MluNI TGGCCA 1 cut(s) 275
MmeI TCCRAC 1 cut(s) 655
Mox20I TGGCCA 1 cut(s) 275
MroI TCCGGA 1 cut(s) 614
MroXI GAANNNNTTC 3 cut(s) 1180, 1754, 1795
MscI TGGCCA 1 cut(s) 275
MseI TTAA 7 cut(s) 126, 1049, 1113, 1311, 1416, 1686, 1824
Msp20I TGGCCA 1 cut(s) 275
MspI CCGG 5 cut(s) 615, 734, 789, 1127, 1500
MspR9I CCNGG 5 cut(s) 521, 1118, 1127, 1305, 1803
Mva1269I GAATGC 2 cut(s) 1008, 1600
MvaI CCWGG 4 cut(s) 521, 1118, 1305, 1803
MvnI CGCG 2 cut(s) 360, 668
MwoI GCNNNNNNNGC 1 cut(s) 962
NciI CCSGG 1 cut(s) 1127
NcoI CCATGG 1 cut(s) 546
NdeII GATC 6 cut(s) 333, 825, 1203, 1453, 1670, 1780
NlaIII CATG 7 cut(s) 182, 325, 550, 710, 725, 1202, 1525
NlaIV GGNNCC 4 cut(s) 610, 1122, 1123, 1741
NmuCI GTSAC 4 cut(s) 736, 853, 874, 1013
NspI RCATGY 1 cut(s) 710
NspV TTCGAA 1 cut(s) 1357
PaeI GCATGC 1 cut(s) 710
PagI TCATGA 1 cut(s) 321
PasI CCCWGGG 1 cut(s) 1802
PceI AGGCCT 1 cut(s) 47
PcsI WCGNNNNNNNCGW 1 cut(s) 678
PctI GAATGC 2 cut(s) 1008, 1600
PdmI GAANNNNTTC 3 cut(s) 1180, 1754, 1795
PfeI GAWTC 4 cut(s) 38, 618, 792, 1729
PflFI GACNNNGTC 1 cut(s) 778
PfoI TCCNGGA 1 cut(s) 1303
PinAI ACCGGT 1 cut(s) 733
PkrI GCNGC 5 cut(s) 184, 329, 427, 511, 687
Ppu21I YACGTR 1 cut(s) 79
PpuMI RGGWCCY 1 cut(s) 1121
Psp124BI GAGCTC 1 cut(s) 1272
Psp1406I AACGTT 1 cut(s) 1045
Psp5II RGGWCCY 1 cut(s) 1121
Psp6I CCWGG 4 cut(s) 519, 1116, 1303, 1801
PspFI CCCAGC 1 cut(s) 421
PspGI CCWGG 4 cut(s) 519, 1116, 1303, 1801
PspN4I GGNNCC 4 cut(s) 610, 1122, 1123, 1741
PspPI GGNCC 4 cut(s) 523, 818, 1121, 1502
PspPPI RGGWCCY 1 cut(s) 1121
PsuI RGATCY 1 cut(s) 1670
PsyI GACNNNGTC 1 cut(s) 778
RsaI GTAC 1 cut(s) 1563
RsaNI GTAC 1 cut(s) 1562
SacI GAGCTC 1 cut(s) 1272
SaqAI TTAA 7 cut(s) 126, 1049, 1113, 1311, 1416, 1686, 1824
SatI GCNGC 5 cut(s) 183, 328, 426, 510, 686
Sau3AI GATC 6 cut(s) 333, 825, 1203, 1453, 1670, 1780
Sau96I GGNCC 4 cut(s) 523, 818, 1121, 1502
ScrFI CCNGG 5 cut(s) 521, 1118, 1127, 1305, 1803
SduI GDGCHC 1 cut(s) 1272
SfaNI GCATC 3 cut(s) 20, 1315, 1449
SfuI TTCGAA 1 cut(s) 1357
SgrAI CRCCGGYG 1 cut(s) 733
SinI GGWCC 2 cut(s) 1121, 1502
SphI GCATGC 1 cut(s) 710
Sse9I AATT 7 cut(s) 280, 960, 1110, 1132, 1176, 1340, 1812
SseBI AGGCCT 1 cut(s) 47
SsiI CCGC 8 cut(s) 328, 352, 358, 426, 668, 798, 1259, 1362
SspI AATATT 2 cut(s) 1232, 1492
SspMI CTAG 3 cut(s) 929, 968, 1511
SstI GAGCTC 1 cut(s) 1272
StuI AGGCCT 1 cut(s) 47
StyD4I CCNGG 5 cut(s) 519, 1116, 1125, 1303, 1801
StyI CCWWGG 2 cut(s) 42, 546
TaaI ACNGT 3 cut(s) 766, 1013, 1588
TaiI ACGT 7 cut(s) 8, 81, 97, 675, 743, 809, 1048
TaqI TCGA 9 cut(s) 530, 570, 621, 696, 782, 1218, 1338, 1357, 1779
TasI AATT 7 cut(s) 280, 960, 1110, 1132, 1176, 1340, 1812
TauI GCSGC 2 cut(s) 330, 428
TfiI GAWTC 4 cut(s) 38, 618, 792, 1729
Tru1I TTAA 7 cut(s) 126, 1049, 1113, 1311, 1416, 1686, 1824
Tru9I TTAA 7 cut(s) 126, 1049, 1113, 1311, 1416, 1686, 1824
TscAI CASTG 5 cut(s) 397, 862, 1018, 1252, 1261
TseFI GTSAC 4 cut(s) 736, 853, 874, 1013
TseI GCWGC 3 cut(s) 182, 509, 685
Tsp45I GTSAC 4 cut(s) 736, 853, 874, 1013
TspDTI ATGAA 7 cut(s) 51, 201, 295, 307, 1538, 1632, 1742
TspGWI ACGGA 4 cut(s) 481, 761, 1398, 1460
TspRI CASTG 5 cut(s) 397, 862, 1018, 1252, 1261
Tth111I GACNNNGTC 1 cut(s) 778
VpaK11BI GGWCC 2 cut(s) 1121, 1502
XapI RAATTY 3 cut(s) 280, 1340, 1812
XceI RCATGY 1 cut(s) 710
XmiI GTMKAC 1 cut(s) 837
XmnI GAANNNNTTC 3 cut(s) 1180, 1754, 1795
XspI CTAG 3 cut(s) 929, 968, 1511
ZraI GACGTC 2 cut(s) 95, 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.