Rh2AG083200

Cytokinin dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013976
Physical Location & Seq
Forward (+)
7047194 .. 7050296
3103 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG083200.1

Sequence Viewer

Length: 1851 bp
ATGACGTTTGCATCAGAAAGAAAAAGAGAGAAGGGAAAGAAAAGAAAGAATGATGAATCCAAGGCCTGTCCTTTGCGTATCTTGGAAAACCAATACGTGTTGAGCTTACGACGTCGTTCCCCACACAATGCCATACACATTTTAAAACGCCAAAGATTTCGTTCTCTGCCAATACAAGATTTTTACCCTCGTTCTCATGCAGCTTTATTTCCCTATATATACCTCTCTTCATCCTTTTATCTCCTACCTACCTACCCATTCCTTCTCTGCCTCACTTTCTCATTTTCAATGGCCAAGAATTCTTCTTCTTTCCCAACATACTTCATAGCCTTATTCATCATGAGCCGCCTGATCCCCACCATCGGATACCGCCTCCGTGACGACCCCGAAGCCATTAGATTAGCTTCCACTGATTACGGCCACATCCACAGCGAAAACCCAGCCGCCGTTCTCTACCCTTCTTCCATAAACGACATTTCGTCCCTCATCAAGTTCGCCAACAATGTTTCCGTGCCGTTTGGCGTGGCTGCGAAAGGCCAGGGCCACTCGACACGGGGACAGGCCATGGCTCGCAATGGGGTCGTGGTCGAAATGTCGTCCCTGCGAAACCACCTACCACAACACGGCTCCTCCGGAATCGAAGTAGTTTCAACCACCAACAAAGACGAAGTGAAGCTTTATTACGCGGACGTCGGAGGCGAGCAGCTCTGGGTCGATGTTCTGCATGCCACATTAGAACATGGACTTTCACCGGTGACGTGGACGGACTACCTTTACTTGACCGTAGGAGGGACGCTGTCGAACGCCGGAATCGGCGGACAGACGTTTCGGTTCGGCCCTCAGATCAGCAATGTCTACGAAATGGATGTTGTCACTGGAGAAGGGGATTTTGTGACTTGCTCCCCAAACAACAACGCAGAGCTTTTCTATGGGGTTCTTGGAGGGCTAGGTCAGTTTGGGGTTATATCCAGAGCCAGAATTGCACTAGAGCCAGCACCAAAAAGGGTCAAGTGGGTGCGAATGCTTTACAGTGACTTCTCTGCGTTTTCCAGAGACCAGGAACGTTTAATCTCAATCAATGGGCGACAACAAAGCAATGCCCTTGATTATTTGGAAGGCTCAATACTAATTAACCAGGGTCCCCCGGATAATTGGAGGGCTTCCTCATTTTTTCCTCAATCCTCTCACAACAGAATTATTTCCAAAGTAAACAAACATGGGATCATATACTGCCTCGAAGTGGTCAAATATTATGACCATCACACTGAAACCACTGCGGATAAGGAGCTCCAAAATCTATTGAAAGGATTGAACTATCTTCCTGGATTTAAGTTTGAAAATGATGCGTCGTATGCCGAATTTCTGAATAGAGTTCGAAGCGGAGAGCTAAAGCTTCAGTCAGAAGGACTATGGGATGTCCCTCATCCGTGGCTTAATCTCTTTATTCCAAAAAGTCGCATCACGGACTTTGATCTTGGTGTGTTCAAGGACATTGTTCTGAAGAGAAATATTACCACCGGACCTGTCCTAGTTTACCCCATGAACAGGAGCAAGTGGAATGAGAGGATGTCGGCCGTTGTACCGGAAGAAGATGTGTTTTACACCGTAGGATTTTTGCATGCAACTGGGTTTGATGAATGGAAGGCATTTGATGAGCAAAATAAAGAGATATTAGGGTTTTGTGTTAGAGCTGGTATTGCTGTTAAGCAGTATCTTCCCGACCACAAAACACAGCAGGATTGGATGAATCATTTTGGCTCCAAATGGAAAACCTTCCAGAAGAGGAAAGCACTATTCGATCCCAAGAGAATACTTTCCCCTGGGCAAAGAATTTTTCAGATTAAAGAATAA

Protein Analysis

616

Amino Acids

70.1

Weight (kDa)

9.23

Isoelectric Point (pI)

53.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 147 - 301 4.5e-14 FAD binding domain
Cytokin-bind PF09265 333 - 612 1e-116 Cytokinin dehydrogenase 1, FAD and cytokinin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56970 AT5G56970
fragaria_vesca FvH4_1g07620
malus_domestica MD15G1208300.v1.1
prunus_persica Prupe.7G208400_v2.0.a1 Prupe.7G208400_v2.0.a1
pyrus_communis pycom15g18450
rosa_chinensis RchiOBHm_Chr2g0093491
rosa_laevigata RLG00000016411
rosa_multiflora Rmu_sc0004423.1_g000008
rosa_roxburghii Rroxscaffold_2G00148040
rosa_rugosa Rorug02G0036700
rosa_samantha Rh2AG083200 Rh2BG083900 Rh2CG085900 Rh2DG082000
rosa_wichuraiana Rw2G006420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 115, 693
AccI GTMKAC 1 cut(s) 855
AccII CGCG 1 cut(s) 686
AccIII TCCGGA 1 cut(s) 632
AciI CCGC 7 cut(s) 346, 370, 444, 686, 816, 1277, 1380
AclI AACGTT 1 cut(s) 1063
AclWI GGATC 3 cut(s) 346, 1229, 1793
AcoI YGGCCR 3 cut(s) 291, 418, 1572
AcsI RAATTY 3 cut(s) 298, 1358, 1830
AcuI CTGAAG 2 cut(s) 1379, 1520
AcyI GRCGYC 2 cut(s) 112, 690
AfaI GTAC 1 cut(s) 1581
AfiI CCNNNNNNNGG 5 cut(s) 362, 623, 789, 1240, 1545
AflIII ACRYGT 1 cut(s) 96
AgeI ACCGGT 1 cut(s) 751
AgsI TTSAA 6 cut(s) 288, 651, 1303, 1312, 1337, 1486
AhdI GACNNNNNGTC 1 cut(s) 478
AjiI CACGTC 1 cut(s) 759
AjnI CCWGG 5 cut(s) 537, 1056, 1134, 1321, 1819
Alw21I GWGCWC 1 cut(s) 1290
Alw26I GTCTC 1 cut(s) 1047
AlwI GGATC 3 cut(s) 346, 1229, 1793
Aor13HI TCCGGA 1 cut(s) 632
AoxI GGCC 8 cut(s) 63, 291, 418, 535, 541, 561, 835, 1572
ApeKI GCWGC 3 cut(s) 200, 527, 703
ApoI RAATTY 3 cut(s) 298, 1358, 1830
ArsI GACNNNNNNTTYG 2 cut(s) 810, 842
AsiGI ACCGGT 1 cut(s) 751
Asp700I GAANNNNTTC 3 cut(s) 1198, 1772, 1813
AspS9I GGNCC 4 cut(s) 541, 836, 1139, 1520
AsuC2I CCSGG 1 cut(s) 1145
AsuHPI GGTGA 2 cut(s) 741, 766
AsuII TTCGAA 1 cut(s) 1375
AvaII GGWCC 2 cut(s) 1139, 1520
BalI TGGCCA 1 cut(s) 293
BanII GRGCYC 1 cut(s) 1290
BarI GAAGNNNNNNTAC 4 cut(s) 665, 697, 1107, 1139
Bbv12I GWGCWC 1 cut(s) 1290
BbvI GCAGC 3 cut(s) 212, 514, 715
BccI CCATC 2 cut(s) 368, 1266
BceAI ACGGC 5 cut(s) 431, 433, 499, 640, 1559
BcgI CGANNNNNNTGC 2 cut(s) 562, 596
BciT130I CCWGG 5 cut(s) 539, 1058, 1136, 1323, 1821
BciVI GTATCC 1 cut(s) 359
BcnI CCSGG 1 cut(s) 1145
BcoDI GTCTC 1 cut(s) 1047
BfaI CTAG 3 cut(s) 947, 986, 1529
BfuI GTATCC 1 cut(s) 359
BisI GCNGC 5 cut(s) 201, 346, 444, 528, 704
BlsI GCNGC 5 cut(s) 202, 347, 445, 529, 705
Bme1390I CCNGG 6 cut(s) 539, 1058, 1136, 1145, 1323, 1821
Bme18I GGWCC 2 cut(s) 1139, 1520
BmeRI GACNNNNNGTC 1 cut(s) 478
BmgBI CACGTC 1 cut(s) 759
BmgT120I GGNCC 4 cut(s) 541, 836, 1139, 1520
BmiI GGNNCC 4 cut(s) 628, 1140, 1141, 1759
BmrFI CCNGG 6 cut(s) 539, 1058, 1136, 1145, 1323, 1821
BmrI ACTGGG 1 cut(s) 1635
BmsI GCATC 3 cut(s) 20, 1333, 1467
BmuI ACTGGG 1 cut(s) 1635
BpmI CTGGAG 1 cut(s) 897
Bpu14I TTCGAA 1 cut(s) 1375
BpuMI CCSGG 1 cut(s) 1145
BsaAI YACGTR 1 cut(s) 97
BsaHI GRCGYC 2 cut(s) 112, 690
BsaI GGTCTC 1 cut(s) 1047
BsaJI CCNNGG 8 cut(s) 60, 538, 564, 1135, 1143, 1427, 1819, 1820
BsaWI WCCGGW 4 cut(s) 632, 751, 1517, 1582
BsaXI ACNNNNNCTCC 2 cut(s) 614, 644
Bsc4I CCNNNNNNNGG 5 cut(s) 362, 623, 789, 1240, 1545
Bse118I RCCGGY 1 cut(s) 751
Bse1I ACTGG 2 cut(s) 880, 1630
Bse3DI GCAATG 3 cut(s) 580, 856, 1102
BseAI TCCGGA 1 cut(s) 632
BseBI CCWGG 5 cut(s) 539, 1058, 1136, 1323, 1821
BseDI CCNNGG 8 cut(s) 60, 538, 564, 1135, 1143, 1427, 1819, 1820
BseGI GGATG 7 cut(s) 230, 423, 871, 1420, 1423, 1572, 1749
BseLI CCNNNNNNNGG 5 cut(s) 362, 623, 789, 1240, 1545
BseMI GCAATG 3 cut(s) 580, 856, 1102
BseMII CTCAG 1 cut(s) 854
BseNI ACTGG 2 cut(s) 880, 1630
BseRI GAGGAG 1 cut(s) 619
BseX3I CGGCCG 1 cut(s) 1572
BseXI GCAGC 3 cut(s) 212, 514, 715
BseYI CCCAGC 1 cut(s) 439
Bsh1236I CGCG 1 cut(s) 686
Bsh1285I CGRYCG 1 cut(s) 1575
BshFI GGCC 8 cut(s) 65, 293, 420, 537, 543, 563, 837, 1574
BshTI ACCGGT 1 cut(s) 751
BsiEI CGRYCG 1 cut(s) 1575
BsiHKAI GWGCWC 1 cut(s) 1290
BsiSI CCGG 6 cut(s) 633, 752, 807, 1145, 1518, 1583
BslFI GGGAC 6 cut(s) 466, 570, 583, 805, 1125, 1403
BslI CCNNNNNNNGG 5 cut(s) 362, 623, 789, 1240, 1545
BsmAI GTCTC 1 cut(s) 1047
BsmFI GGGAC 6 cut(s) 466, 570, 583, 805, 1125, 1403
BsmI GAATGC 1 cut(s) 1026
BsnI GGCC 8 cut(s) 65, 293, 420, 537, 543, 563, 837, 1574
Bso31I GGTCTC 1 cut(s) 1047
Bsp119I TTCGAA 1 cut(s) 1375
Bsp1286I GDGCHC 1 cut(s) 1290
Bsp13I TCCGGA 1 cut(s) 632
Bsp143I GATC 5 cut(s) 351, 843, 1221, 1471, 1798
Bsp19I CCATGG 1 cut(s) 564
BspACI CCGC 7 cut(s) 346, 370, 444, 686, 816, 1277, 1380
BspANI GGCC 8 cut(s) 65, 293, 420, 537, 543, 563, 837, 1574
BspCNI CTCAG 1 cut(s) 853
BspEI TCCGGA 1 cut(s) 632
BspFNI CGCG 1 cut(s) 686
BspHI TCATGA 1 cut(s) 339
BspLI GGNNCC 4 cut(s) 628, 1140, 1141, 1759
BspPI GGATC 3 cut(s) 346, 1229, 1793
BspT104I TTCGAA 1 cut(s) 1375
BspTNI GGTCTC 1 cut(s) 1047
BsrDI GCAATG 3 cut(s) 580, 856, 1102
BsrFI RCCGGY 1 cut(s) 751
BsrI ACTGG 2 cut(s) 880, 1630
BssAI RCCGGY 1 cut(s) 751
BssECI CCNNGG 8 cut(s) 60, 538, 564, 1135, 1143, 1427, 1819, 1820
BssMI GATC 5 cut(s) 351, 843, 1221, 1471, 1798
BssNI GRCGYC 2 cut(s) 112, 690
BssT1I CCWWGG 2 cut(s) 60, 564
Bst2UI CCWGG 5 cut(s) 539, 1058, 1136, 1323, 1821
Bst4CI ACNGT 3 cut(s) 784, 1031, 1606
Bst6I CTCTTC 3 cut(s) 232, 1496, 1775
BstACI GRCGYC 2 cut(s) 112, 690
BstBAI YACGTR 1 cut(s) 97
BstBI TTCGAA 1 cut(s) 1375
BstC8I GCNNGC 5 cut(s) 571, 701, 726, 993, 1620
BstDEI CTNAG 1 cut(s) 840
BstDSI CCRYGG 2 cut(s) 564, 1427
BstF5I GGATG 7 cut(s) 230, 423, 871, 1420, 1423, 1572, 1749
BstFNI CGCG 1 cut(s) 686
BstKTI GATC 5 cut(s) 354, 846, 1224, 1474, 1801
BstMAI GTCTC 1 cut(s) 1047
BstMBI GATC 5 cut(s) 351, 843, 1221, 1471, 1798
BstMCI CGRYCG 1 cut(s) 1575
BstMWI GCNNNNNNNGC 3 cut(s) 980, 1352, 1697
BstNI CCWGG 5 cut(s) 539, 1058, 1136, 1323, 1821
BstNSI RCATGY 2 cut(s) 728, 1622
BstSCI CCNGG 6 cut(s) 537, 1056, 1134, 1143, 1321, 1819
BstUI CGCG 1 cut(s) 686
BstV1I GCAGC 3 cut(s) 212, 514, 715
BstZI CGGCCG 1 cut(s) 1572
BsuI GTATCC 1 cut(s) 359
BsuRI GGCC 8 cut(s) 65, 293, 420, 537, 543, 563, 837, 1574
BtgI CCRYGG 2 cut(s) 564, 1427
BtrI CACGTC 1 cut(s) 759
BtsCI GGATG 7 cut(s) 230, 423, 871, 1420, 1423, 1572, 1749
BtsI GCAGTG 1 cut(s) 1272
BtsIMutI CAGTG 5 cut(s) 408, 873, 1036, 1263, 1272
Cac8I GCNNGC 5 cut(s) 571, 701, 726, 993, 1620
CciI TCATGA 1 cut(s) 339
Cfr10I RCCGGY 1 cut(s) 751
Cfr13I GGNCC 4 cut(s) 541, 836, 1139, 1520
CseI GACGC 2 cut(s) 802, 1335
Csp6I GTAC 1 cut(s) 1580
CspAI ACCGGT 1 cut(s) 751
CviAII CATG 8 cut(s) 197, 340, 565, 725, 740, 1217, 1540, 1619
CviQI GTAC 1 cut(s) 1580
DdeI CTNAG 1 cut(s) 840
DpnI GATC 5 cut(s) 353, 845, 1223, 1473, 1800
DpnII GATC 5 cut(s) 351, 843, 1221, 1471, 1798
DraI TTTAAA 1 cut(s) 144
DriI GACNNNNNGTC 1 cut(s) 478
EaeI YGGCCR 3 cut(s) 291, 418, 1572
EagI CGGCCG 1 cut(s) 1572
Eam1104I CTCTTC 3 cut(s) 232, 1496, 1775
Eam1105I GACNNNNNGTC 1 cut(s) 478
EarI CTCTTC 3 cut(s) 232, 1496, 1775
EciI GGCGGA 1 cut(s) 831
Ecl136II GAGCTC 1 cut(s) 1288
EclXI CGGCCG 1 cut(s) 1572
Eco130I CCWWGG 2 cut(s) 60, 564
Eco147I AGGCCT 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 1290
Eco31I GGTCTC 1 cut(s) 1047
Eco47I GGWCC 2 cut(s) 1139, 1520
Eco52I CGGCCG 1 cut(s) 1572
Eco53kI GAGCTC 1 cut(s) 1288
Eco57I CTGAAG 2 cut(s) 1379, 1520
EcoICRI GAGCTC 1 cut(s) 1288
EcoO109I RGGNCCY 1 cut(s) 1139
EcoRI GAATTC 1 cut(s) 298
EcoRII CCWGG 5 cut(s) 537, 1056, 1134, 1321, 1819
EcoT14I CCWWGG 2 cut(s) 60, 564
EcoT38I GRGCYC 1 cut(s) 1290
ErhI CCWWGG 2 cut(s) 60, 564
FaeI CATG 8 cut(s) 200, 343, 568, 728, 743, 1220, 1543, 1622
FalI AAGNNNNNCTT 2 cut(s) 660, 692
FaqI GGGAC 6 cut(s) 466, 570, 583, 805, 1125, 1403
FatI CATG 8 cut(s) 196, 339, 564, 724, 739, 1216, 1539, 1618
FblI GTMKAC 1 cut(s) 855
Fnu4HI GCNGC 5 cut(s) 201, 346, 444, 528, 704
FokI GGATG 7 cut(s) 217, 410, 878, 1410, 1427, 1579, 1756
FriOI GRGCYC 1 cut(s) 1290
Fsp4HI GCNGC 5 cut(s) 201, 346, 444, 528, 704
FspBI CTAG 3 cut(s) 947, 986, 1529
GluI GCNGC 5 cut(s) 201, 346, 444, 528, 704
GsaI CCCAGC 1 cut(s) 443
GsuI CTGGAG 1 cut(s) 897
HaeIII GGCC 8 cut(s) 65, 293, 420, 537, 543, 563, 837, 1574
HapII CCGG 6 cut(s) 633, 752, 807, 1145, 1518, 1583
HgaI GACGC 2 cut(s) 802, 1335
Hin1I GRCGYC 2 cut(s) 112, 690
Hin1II CATG 8 cut(s) 200, 343, 568, 728, 743, 1220, 1543, 1622
HindIII AAGCTT 2 cut(s) 674, 1391
HinfI GANTC 4 cut(s) 56, 636, 810, 1747
HpaII CCGG 6 cut(s) 633, 752, 807, 1145, 1518, 1583
HphI GGTGA 2 cut(s) 741, 766
Hpy166II GTNNAC 4 cut(s) 762, 856, 1210, 1534
Hpy188I TCNGA 8 cut(s) 16, 365, 695, 843, 1365, 1402, 1500, 1839
Hpy188III TCNNGA 6 cut(s) 340, 633, 969, 1050, 1718, 1777
Hpy8I GTNNAC 4 cut(s) 762, 856, 1210, 1534
Hpy99I CGWCG 4 cut(s) 114, 117, 695, 1351
HpyAV CCTTC 8 cut(s) 25, 272, 468, 875, 1109, 1397, 1636, 1783
HpyCH4III ACNGT 3 cut(s) 784, 1031, 1606
HpyCH4IV ACGT 7 cut(s) 5, 96, 112, 690, 758, 824, 1063
HpyCH4V TGCA 6 cut(s) 11, 200, 724, 983, 1618, 1622
HpyF10VI GCNNNNNNNGC 3 cut(s) 980, 1352, 1697
HpyF3I CTNAG 1 cut(s) 840
HpySE526I ACGT 7 cut(s) 5, 96, 112, 690, 758, 824, 1063
Hsp92I GRCGYC 2 cut(s) 112, 690
Hsp92II CATG 8 cut(s) 200, 343, 568, 728, 743, 1220, 1543, 1622
KflI GGGWCCC 1 cut(s) 1139
Kpn2I TCCGGA 1 cut(s) 632
Kzo9I GATC 5 cut(s) 351, 843, 1221, 1471, 1798
LmnI GCTCC 6 cut(s) 632, 905, 1285, 1293, 1548, 1763
Lsp1109I GCAGC 3 cut(s) 212, 514, 715
LweI GCATC 3 cut(s) 20, 1333, 1467
MaeI CTAG 3 cut(s) 947, 986, 1529
MaeII ACGT 7 cut(s) 5, 96, 112, 690, 758, 824, 1063
MaeIII GTNAC 5 cut(s) 377, 754, 871, 892, 1031
MalI GATC 5 cut(s) 353, 845, 1223, 1473, 1800
MboI GATC 5 cut(s) 351, 843, 1221, 1471, 1798
MhlI GDGCHC 1 cut(s) 1290
MlsI TGGCCA 1 cut(s) 293
MluCI AATT 7 cut(s) 298, 978, 1128, 1150, 1194, 1358, 1830
MluNI TGGCCA 1 cut(s) 293
MmeI TCCRAC 1 cut(s) 673
Mox20I TGGCCA 1 cut(s) 293
MroI TCCGGA 1 cut(s) 632
MroXI GAANNNNTTC 3 cut(s) 1198, 1772, 1813
MscI TGGCCA 1 cut(s) 293
MseI TTAA 7 cut(s) 143, 1067, 1131, 1329, 1434, 1704, 1842
Msp20I TGGCCA 1 cut(s) 293
MspI CCGG 6 cut(s) 633, 752, 807, 1145, 1518, 1583
MspR9I CCNGG 6 cut(s) 539, 1058, 1136, 1145, 1323, 1821
Mva1269I GAATGC 1 cut(s) 1026
MvaI CCWGG 5 cut(s) 539, 1058, 1136, 1323, 1821
MvnI CGCG 1 cut(s) 686
MwoI GCNNNNNNNGC 3 cut(s) 980, 1352, 1697
NciI CCSGG 1 cut(s) 1145
NcoI CCATGG 1 cut(s) 564
NdeII GATC 5 cut(s) 351, 843, 1221, 1471, 1798
NlaIII CATG 8 cut(s) 200, 343, 568, 728, 743, 1220, 1543, 1622
NlaIV GGNNCC 4 cut(s) 628, 1140, 1141, 1759
NmuCI GTSAC 5 cut(s) 377, 754, 871, 892, 1031
NspI RCATGY 2 cut(s) 728, 1622
NspV TTCGAA 1 cut(s) 1375
PaeI GCATGC 2 cut(s) 728, 1622
PagI TCATGA 1 cut(s) 339
PasI CCCWGGG 1 cut(s) 1820
PceI AGGCCT 1 cut(s) 65
PcsI WCGNNNNNNNCGW 1 cut(s) 696
PctI GAATGC 1 cut(s) 1026
PdmI GAANNNNTTC 3 cut(s) 1198, 1772, 1813
PfeI GAWTC 4 cut(s) 56, 636, 810, 1747
PflFI GACNNNGTC 1 cut(s) 796
PfoI TCCNGGA 1 cut(s) 1321
PinAI ACCGGT 1 cut(s) 751
PkrI GCNGC 5 cut(s) 202, 347, 445, 529, 705
Ppu21I YACGTR 1 cut(s) 97
PpuMI RGGWCCY 1 cut(s) 1139
Psp124BI GAGCTC 1 cut(s) 1290
Psp1406I AACGTT 1 cut(s) 1063
Psp5II RGGWCCY 1 cut(s) 1139
Psp6I CCWGG 5 cut(s) 537, 1056, 1134, 1321, 1819
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 5 cut(s) 537, 1056, 1134, 1321, 1819
PspN4I GGNNCC 4 cut(s) 628, 1140, 1141, 1759
PspPI GGNCC 4 cut(s) 541, 836, 1139, 1520
PspPPI RGGWCCY 1 cut(s) 1139
PsyI GACNNNGTC 1 cut(s) 796
RsaI GTAC 1 cut(s) 1581
RsaNI GTAC 1 cut(s) 1580
SacI GAGCTC 1 cut(s) 1290
SaqAI TTAA 7 cut(s) 143, 1067, 1131, 1329, 1434, 1704, 1842
SatI GCNGC 5 cut(s) 201, 346, 444, 528, 704
Sau3AI GATC 5 cut(s) 351, 843, 1221, 1471, 1798
Sau96I GGNCC 4 cut(s) 541, 836, 1139, 1520
ScrFI CCNGG 6 cut(s) 539, 1058, 1136, 1145, 1323, 1821
SduI GDGCHC 1 cut(s) 1290
SfaNI GCATC 3 cut(s) 20, 1333, 1467
SfuI TTCGAA 1 cut(s) 1375
SgrAI CRCCGGYG 1 cut(s) 751
SinI GGWCC 2 cut(s) 1139, 1520
SphI GCATGC 2 cut(s) 728, 1622
Sse9I AATT 7 cut(s) 298, 978, 1128, 1150, 1194, 1358, 1830
SseBI AGGCCT 1 cut(s) 65
SsiI CCGC 7 cut(s) 346, 370, 444, 686, 816, 1277, 1380
SspI AATATT 2 cut(s) 1250, 1510
SspMI CTAG 3 cut(s) 947, 986, 1529
SstI GAGCTC 1 cut(s) 1290
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 6 cut(s) 537, 1056, 1134, 1143, 1321, 1819
StyI CCWWGG 2 cut(s) 60, 564
TaaI ACNGT 3 cut(s) 784, 1031, 1606
TaiI ACGT 7 cut(s) 8, 99, 115, 693, 761, 827, 1066
TaqI TCGA 8 cut(s) 548, 588, 639, 714, 800, 1236, 1375, 1797
TasI AATT 7 cut(s) 298, 978, 1128, 1150, 1194, 1358, 1830
TauI GCSGC 2 cut(s) 348, 446
TfiI GAWTC 4 cut(s) 56, 636, 810, 1747
Tru1I TTAA 7 cut(s) 143, 1067, 1131, 1329, 1434, 1704, 1842
Tru9I TTAA 7 cut(s) 143, 1067, 1131, 1329, 1434, 1704, 1842
TscAI CASTG 5 cut(s) 415, 880, 1036, 1270, 1279
TseFI GTSAC 5 cut(s) 377, 754, 871, 892, 1031
TseI GCWGC 3 cut(s) 200, 527, 703
Tsp45I GTSAC 5 cut(s) 377, 754, 871, 892, 1031
TspDTI ATGAA 7 cut(s) 69, 219, 313, 325, 1556, 1650, 1760
TspGWI ACGGA 5 cut(s) 365, 499, 779, 1416, 1478
TspRI CASTG 5 cut(s) 415, 880, 1036, 1270, 1279
Tth111I GACNNNGTC 1 cut(s) 796
VpaK11BI GGWCC 2 cut(s) 1139, 1520
XapI RAATTY 3 cut(s) 298, 1358, 1830
XceI RCATGY 2 cut(s) 728, 1622
XmiI GTMKAC 1 cut(s) 855
XmnI GAANNNNTTC 3 cut(s) 1198, 1772, 1813
XspI CTAG 3 cut(s) 947, 986, 1529
ZraI GACGTC 2 cut(s) 113, 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.