Rmu_sc0004423.1_g000008

Cytokinin dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004423.1
OG0013976
Physical Location & Seq
Reverse (-)
36100 .. 38420
2321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004423.1_g000008.1.cds

Sequence Viewer

Length: 1218 bp
atgagccgcctgatccccaccatcggataccgcctccgtgacgaccccgaagccattagattagcttccactgattacggccacatccacagcgaaaaccctgccgccgttctctacccttcttccataaacgacatttcgtccctcatcaagttcgccaacaatgtttccgtgccgtttggcgtggctgcgaaaggccagggccactcgacacggggacaggccatggctcgcaatggggtcgtggtcgaaatgtcgtccctgcgaaaccacctaccacaacacggctcctccggaatcgaagtagtttcaaccaccaacaaagacgaagtgaagctttattacgcggacgtcggaggcgagcagctctgggtcgatgttctgcatgccacattagaacatggactttcaccggtgacgtggacggactacctttacttgaccgtaggagggacgctgtcgaacgccggaatcggcggacagacgtttcggttcggccctcagatcagcaatgtctacgaaatggatgttgtcactggagaaggggattttgtgacttgctccccaaacaacaacgcagagcttttctatggggttcttggagggctaggtcagtttggggttatatccagagccagaattgcactagagccagcaccaaaaagggtcaagtgggtgcgaatgctttacagtgacttctctgcgttttccagagaccaggaacgtttaatctcaatcaatgggcgacaacaaagcaatgcccttgattatttggaaggctcaatactaattaaccagggtcccccggataattggagggcttcctcattttttcctcaatcctctcacaacagaattatttccaaagtaaacaaacatgggatcatatactgcctcgaagtggtcaaatattatgaccatcacactgaaaccactgcggataaggagctccaaaatctattgaaaggattgaactatcttcctggatttaagtttgaaaatgatgcgtcgtatgccgaatttctgaatagagttcgaagcggagagctaaagcttcagtcagaaggactatgggatgtccctcatccgtggcttaatctctttattccaaaaagtcgcatcacggactttgatcttggtgtgttcaaggacattgttctgaagagaaatattaccaccggacctgtcctagtttaccccatgaacaggagcaagtaa

Protein Analysis

405

Amino Acids

45.08

Weight (kDa)

6.64

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56970 AT5G56970
fragaria_vesca FvH4_1g07620
malus_domestica MD15G1208300.v1.1
prunus_persica Prupe.7G208400_v2.0.a1 Prupe.7G208400_v2.0.a1
pyrus_communis pycom15g18450
rosa_chinensis RchiOBHm_Chr2g0093491
rosa_laevigata RLG00000016411
rosa_multiflora Rmu_sc0004423.1_g000008
rosa_roxburghii Rroxscaffold_2G00148040
rosa_rugosa Rorug02G0036700
rosa_samantha Rh2AG083200 Rh2BG083900 Rh2CG085900 Rh2DG082000
rosa_wichuraiana Rw2G006420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 354
AccI GTMKAC 1 cut(s) 516
AccII CGCG 1 cut(s) 347
AccIII TCCGGA 1 cut(s) 293
AciI CCGC 7 cut(s) 7, 31, 105, 347, 477, 938, 1041
AclI AACGTT 1 cut(s) 724
AclWI GGATC 2 cut(s) 7, 890
AcoI YGGCCR 1 cut(s) 79
AcsI RAATTY 1 cut(s) 1019
AcuI CTGAAG 2 cut(s) 1040, 1181
AcyI GRCGYC 1 cut(s) 351
AfiI CCNNNNNNNGG 5 cut(s) 23, 284, 450, 901, 1206
AgeI ACCGGT 1 cut(s) 412
AgsI TTSAA 5 cut(s) 312, 964, 973, 998, 1147
AhdI GACNNNNNGTC 1 cut(s) 139
AjiI CACGTC 1 cut(s) 420
AjnI CCWGG 4 cut(s) 198, 717, 795, 982
AluBI AGCT 7 cut(s) 65, 337, 367, 583, 949, 1048, 1054
AluI AGCT 7 cut(s) 65, 337, 367, 583, 949, 1048, 1054
Alw21I GWGCWC 1 cut(s) 951
Alw26I GTCTC 1 cut(s) 708
AlwI GGATC 2 cut(s) 7, 890
Aor13HI TCCGGA 1 cut(s) 293
AoxI GGCC 5 cut(s) 79, 196, 202, 222, 496
ApeKI GCWGC 2 cut(s) 188, 364
ApoI RAATTY 1 cut(s) 1019
ArsI GACNNNNNNTTYG 2 cut(s) 471, 503
AsiGI ACCGGT 1 cut(s) 412
Asp700I GAANNNNTTC 1 cut(s) 859
AspS9I GGNCC 4 cut(s) 202, 497, 800, 1181
AsuC2I CCSGG 1 cut(s) 806
AsuHPI GGTGA 2 cut(s) 402, 427
AsuII TTCGAA 1 cut(s) 1036
AvaII GGWCC 2 cut(s) 800, 1181
BanII GRGCYC 1 cut(s) 951
BarI GAAGNNNNNNTAC 4 cut(s) 326, 358, 768, 800
Bbv12I GWGCWC 1 cut(s) 951
BbvI GCAGC 2 cut(s) 175, 376
BccI CCATC 2 cut(s) 29, 927
BceAI ACGGC 4 cut(s) 92, 94, 160, 301
BcgI CGANNNNNNTGC 4 cut(s) 83, 117, 223, 257
BciT130I CCWGG 4 cut(s) 200, 719, 797, 984
BciVI GTATCC 1 cut(s) 20
BcnI CCSGG 1 cut(s) 806
BcoDI GTCTC 1 cut(s) 708
BfaI CTAG 3 cut(s) 608, 647, 1190
BfuI GTATCC 1 cut(s) 20
BisI GCNGC 4 cut(s) 7, 105, 189, 365
BlsI GCNGC 4 cut(s) 8, 106, 190, 366
Bme1390I CCNGG 5 cut(s) 200, 719, 797, 806, 984
Bme18I GGWCC 2 cut(s) 800, 1181
BmeRI GACNNNNNGTC 1 cut(s) 139
BmgBI CACGTC 1 cut(s) 420
BmgT120I GGNCC 4 cut(s) 202, 497, 800, 1181
BmiI GGNNCC 3 cut(s) 289, 801, 802
BmrFI CCNGG 5 cut(s) 200, 719, 797, 806, 984
BmsI GCATC 2 cut(s) 994, 1128
BpmI CTGGAG 1 cut(s) 558
Bpu14I TTCGAA 1 cut(s) 1036
BpuMI CCSGG 1 cut(s) 806
BsaHI GRCGYC 1 cut(s) 351
BsaI GGTCTC 1 cut(s) 708
BsaJI CCNNGG 5 cut(s) 199, 225, 796, 804, 1088
BsaWI WCCGGW 3 cut(s) 293, 412, 1178
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bsc4I CCNNNNNNNGG 5 cut(s) 23, 284, 450, 901, 1206
Bse118I RCCGGY 1 cut(s) 412
Bse1I ACTGG 1 cut(s) 541
Bse3DI GCAATG 3 cut(s) 241, 517, 763
BseAI TCCGGA 1 cut(s) 293
BseBI CCWGG 4 cut(s) 200, 719, 797, 984
BseDI CCNNGG 5 cut(s) 199, 225, 796, 804, 1088
BseGI GGATG 4 cut(s) 84, 532, 1081, 1084
BseLI CCNNNNNNNGG 5 cut(s) 23, 284, 450, 901, 1206
BseMI GCAATG 3 cut(s) 241, 517, 763
BseMII CTCAG 1 cut(s) 515
BseNI ACTGG 1 cut(s) 541
BseRI GAGGAG 1 cut(s) 280
BseXI GCAGC 2 cut(s) 175, 376
Bsh1236I CGCG 1 cut(s) 347
BshFI GGCC 5 cut(s) 81, 198, 204, 224, 498
BshTI ACCGGT 1 cut(s) 412
BsiHKAI GWGCWC 1 cut(s) 951
BsiSI CCGG 5 cut(s) 294, 413, 468, 806, 1179
BslFI GGGAC 6 cut(s) 127, 231, 244, 466, 786, 1064
BslI CCNNNNNNNGG 5 cut(s) 23, 284, 450, 901, 1206
BsmAI GTCTC 1 cut(s) 708
BsmFI GGGAC 6 cut(s) 127, 231, 244, 466, 786, 1064
BsmI GAATGC 1 cut(s) 687
BsnI GGCC 5 cut(s) 81, 198, 204, 224, 498
Bso31I GGTCTC 1 cut(s) 708
Bsp119I TTCGAA 1 cut(s) 1036
Bsp1286I GDGCHC 1 cut(s) 951
Bsp13I TCCGGA 1 cut(s) 293
Bsp143I GATC 4 cut(s) 12, 504, 882, 1132
Bsp19I CCATGG 1 cut(s) 225
BspACI CCGC 7 cut(s) 7, 31, 105, 347, 477, 938, 1041
BspANI GGCC 5 cut(s) 81, 198, 204, 224, 498
BspCNI CTCAG 1 cut(s) 514
BspEI TCCGGA 1 cut(s) 293
BspFNI CGCG 1 cut(s) 347
BspLI GGNNCC 3 cut(s) 289, 801, 802
BspPI GGATC 2 cut(s) 7, 890
BspT104I TTCGAA 1 cut(s) 1036
BspTNI GGTCTC 1 cut(s) 708
BsrDI GCAATG 3 cut(s) 241, 517, 763
BsrFI RCCGGY 1 cut(s) 412
BsrI ACTGG 1 cut(s) 541
BssAI RCCGGY 1 cut(s) 412
BssECI CCNNGG 5 cut(s) 199, 225, 796, 804, 1088
BssMI GATC 4 cut(s) 12, 504, 882, 1132
BssNI GRCGYC 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 225
Bst2UI CCWGG 4 cut(s) 200, 719, 797, 984
Bst4CI ACNGT 2 cut(s) 445, 692
Bst6I CTCTTC 1 cut(s) 1157
BstACI GRCGYC 1 cut(s) 351
BstBI TTCGAA 1 cut(s) 1036
BstC8I GCNNGC 4 cut(s) 232, 362, 387, 654
BstDEI CTNAG 1 cut(s) 501
BstDSI CCRYGG 2 cut(s) 225, 1088
BstF5I GGATG 4 cut(s) 84, 532, 1081, 1084
BstFNI CGCG 1 cut(s) 347
BstKTI GATC 4 cut(s) 15, 507, 885, 1135
BstMAI GTCTC 1 cut(s) 708
BstMBI GATC 4 cut(s) 12, 504, 882, 1132
BstMWI GCNNNNNNNGC 2 cut(s) 641, 1013
BstNI CCWGG 4 cut(s) 200, 719, 797, 984
BstNSI RCATGY 1 cut(s) 389
BstSCI CCNGG 5 cut(s) 198, 717, 795, 804, 982
BstUI CGCG 1 cut(s) 347
BstV1I GCAGC 2 cut(s) 175, 376
BsuI GTATCC 1 cut(s) 20
BsuRI GGCC 5 cut(s) 81, 198, 204, 224, 498
BtgI CCRYGG 2 cut(s) 225, 1088
BtrI CACGTC 1 cut(s) 420
BtsCI GGATG 4 cut(s) 84, 532, 1081, 1084
BtsI GCAGTG 1 cut(s) 933
BtsIMutI CAGTG 5 cut(s) 69, 534, 697, 924, 933
Cac8I GCNNGC 4 cut(s) 232, 362, 387, 654
Cfr10I RCCGGY 1 cut(s) 412
Cfr13I GGNCC 4 cut(s) 202, 497, 800, 1181
CseI GACGC 2 cut(s) 463, 996
CspAI ACCGGT 1 cut(s) 412
CviAII CATG 5 cut(s) 226, 386, 401, 878, 1201
DdeI CTNAG 1 cut(s) 501
DpnI GATC 4 cut(s) 14, 506, 884, 1134
DpnII GATC 4 cut(s) 12, 504, 882, 1132
DriI GACNNNNNGTC 1 cut(s) 139
EaeI YGGCCR 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 1157
Eam1105I GACNNNNNGTC 1 cut(s) 139
EarI CTCTTC 1 cut(s) 1157
EciI GGCGGA 1 cut(s) 492
Ecl136II GAGCTC 1 cut(s) 949
Eco130I CCWWGG 1 cut(s) 225
Eco24I GRGCYC 1 cut(s) 951
Eco31I GGTCTC 1 cut(s) 708
Eco47I GGWCC 2 cut(s) 800, 1181
Eco53kI GAGCTC 1 cut(s) 949
Eco57I CTGAAG 2 cut(s) 1040, 1181
EcoICRI GAGCTC 1 cut(s) 949
EcoO109I RGGNCCY 1 cut(s) 800
EcoRII CCWGG 4 cut(s) 198, 717, 795, 982
EcoT14I CCWWGG 1 cut(s) 225
EcoT38I GRGCYC 1 cut(s) 951
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 5 cut(s) 229, 389, 404, 881, 1204
FalI AAGNNNNNCTT 2 cut(s) 321, 353
FaqI GGGAC 6 cut(s) 127, 231, 244, 466, 786, 1064
FatI CATG 5 cut(s) 225, 385, 400, 877, 1200
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 4 cut(s) 7, 105, 189, 365
FokI GGATG 4 cut(s) 71, 539, 1071, 1088
FriOI GRGCYC 1 cut(s) 951
Fsp4HI GCNGC 4 cut(s) 7, 105, 189, 365
FspBI CTAG 3 cut(s) 608, 647, 1190
GluI GCNGC 4 cut(s) 7, 105, 189, 365
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 5 cut(s) 81, 198, 204, 224, 498
HapII CCGG 5 cut(s) 294, 413, 468, 806, 1179
HgaI GACGC 2 cut(s) 463, 996
Hin1I GRCGYC 1 cut(s) 351
Hin1II CATG 5 cut(s) 229, 389, 404, 881, 1204
HindIII AAGCTT 2 cut(s) 335, 1052
HinfI GANTC 2 cut(s) 297, 471
HpaII CCGG 5 cut(s) 294, 413, 468, 806, 1179
HphI GGTGA 2 cut(s) 402, 427
Hpy166II GTNNAC 4 cut(s) 423, 517, 871, 1195
Hpy188I TCNGA 6 cut(s) 26, 356, 504, 1026, 1063, 1161
Hpy188III TCNNGA 3 cut(s) 294, 630, 711
Hpy8I GTNNAC 4 cut(s) 423, 517, 871, 1195
Hpy99I CGWCG 2 cut(s) 356, 1012
HpyAV CCTTC 4 cut(s) 129, 536, 770, 1058
HpyCH4III ACNGT 2 cut(s) 445, 692
HpyCH4IV ACGT 4 cut(s) 351, 419, 485, 724
HpyCH4V TGCA 2 cut(s) 385, 644
HpyF10VI GCNNNNNNNGC 2 cut(s) 641, 1013
HpyF3I CTNAG 1 cut(s) 501
HpySE526I ACGT 4 cut(s) 351, 419, 485, 724
Hsp92I GRCGYC 1 cut(s) 351
Hsp92II CATG 5 cut(s) 229, 389, 404, 881, 1204
KflI GGGWCCC 1 cut(s) 800
Kpn2I TCCGGA 1 cut(s) 293
Kzo9I GATC 4 cut(s) 12, 504, 882, 1132
LmnI GCTCC 5 cut(s) 293, 566, 946, 954, 1209
Lsp1109I GCAGC 2 cut(s) 175, 376
LweI GCATC 2 cut(s) 994, 1128
MaeI CTAG 3 cut(s) 608, 647, 1190
MaeII ACGT 4 cut(s) 351, 419, 485, 724
MaeIII GTNAC 5 cut(s) 38, 415, 532, 553, 692
MalI GATC 4 cut(s) 14, 506, 884, 1134
MboI GATC 4 cut(s) 12, 504, 882, 1132
MboII GAAGA 3 cut(s) 114, 971, 1174
MhlI GDGCHC 1 cut(s) 951
MluCI AATT 5 cut(s) 639, 789, 811, 855, 1019
MmeI TCCRAC 1 cut(s) 334
MroI TCCGGA 1 cut(s) 293
MroXI GAANNNNTTC 1 cut(s) 859
MseI TTAA 4 cut(s) 728, 792, 990, 1095
MspI CCGG 5 cut(s) 294, 413, 468, 806, 1179
MspR9I CCNGG 5 cut(s) 200, 719, 797, 806, 984
Mva1269I GAATGC 1 cut(s) 687
MvaI CCWGG 4 cut(s) 200, 719, 797, 984
MvnI CGCG 1 cut(s) 347
MwoI GCNNNNNNNGC 2 cut(s) 641, 1013
NciI CCSGG 1 cut(s) 806
NcoI CCATGG 1 cut(s) 225
NdeII GATC 4 cut(s) 12, 504, 882, 1132
NlaIII CATG 5 cut(s) 229, 389, 404, 881, 1204
NlaIV GGNNCC 3 cut(s) 289, 801, 802
NmuCI GTSAC 5 cut(s) 38, 415, 532, 553, 692
NspI RCATGY 1 cut(s) 389
NspV TTCGAA 1 cut(s) 1036
PaeI GCATGC 1 cut(s) 389
PcsI WCGNNNNNNNCGW 1 cut(s) 357
PctI GAATGC 1 cut(s) 687
PdmI GAANNNNTTC 1 cut(s) 859
PfeI GAWTC 2 cut(s) 297, 471
PflFI GACNNNGTC 1 cut(s) 457
PfoI TCCNGGA 1 cut(s) 982
PinAI ACCGGT 1 cut(s) 412
PkrI GCNGC 4 cut(s) 8, 106, 190, 366
PpuMI RGGWCCY 1 cut(s) 800
Psp124BI GAGCTC 1 cut(s) 951
Psp1406I AACGTT 1 cut(s) 724
Psp5II RGGWCCY 1 cut(s) 800
Psp6I CCWGG 4 cut(s) 198, 717, 795, 982
PspGI CCWGG 4 cut(s) 198, 717, 795, 982
PspN4I GGNNCC 3 cut(s) 289, 801, 802
PspPI GGNCC 4 cut(s) 202, 497, 800, 1181
PspPPI RGGWCCY 1 cut(s) 800
PsyI GACNNNGTC 1 cut(s) 457
SacI GAGCTC 1 cut(s) 951
SaqAI TTAA 4 cut(s) 728, 792, 990, 1095
SatI GCNGC 4 cut(s) 7, 105, 189, 365
Sau3AI GATC 4 cut(s) 12, 504, 882, 1132
Sau96I GGNCC 4 cut(s) 202, 497, 800, 1181
ScrFI CCNGG 5 cut(s) 200, 719, 797, 806, 984
SduI GDGCHC 1 cut(s) 951
SfaNI GCATC 2 cut(s) 994, 1128
SfuI TTCGAA 1 cut(s) 1036
SgrAI CRCCGGYG 1 cut(s) 412
SinI GGWCC 2 cut(s) 800, 1181
SphI GCATGC 1 cut(s) 389
Sse9I AATT 5 cut(s) 639, 789, 811, 855, 1019
SsiI CCGC 7 cut(s) 7, 31, 105, 347, 477, 938, 1041
SspI AATATT 2 cut(s) 911, 1171
SspMI CTAG 3 cut(s) 608, 647, 1190
SstI GAGCTC 1 cut(s) 951
StyD4I CCNGG 5 cut(s) 198, 717, 795, 804, 982
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 2 cut(s) 445, 692
TaiI ACGT 4 cut(s) 354, 422, 488, 727
TaqI TCGA 7 cut(s) 209, 249, 300, 375, 461, 897, 1036
TasI AATT 5 cut(s) 639, 789, 811, 855, 1019
TauI GCSGC 2 cut(s) 9, 107
TfiI GAWTC 2 cut(s) 297, 471
Tru1I TTAA 4 cut(s) 728, 792, 990, 1095
Tru9I TTAA 4 cut(s) 728, 792, 990, 1095
TscAI CASTG 5 cut(s) 76, 541, 697, 931, 940
TseFI GTSAC 5 cut(s) 38, 415, 532, 553, 692
TseI GCWGC 2 cut(s) 188, 364
Tsp45I GTSAC 5 cut(s) 38, 415, 532, 553, 692
TspDTI ATGAA 1 cut(s) 1217
TspGWI ACGGA 5 cut(s) 26, 160, 440, 1077, 1139
TspRI CASTG 5 cut(s) 76, 541, 697, 931, 940
Tth111I GACNNNGTC 1 cut(s) 457
VpaK11BI GGWCC 2 cut(s) 800, 1181
XapI RAATTY 1 cut(s) 1019
XceI RCATGY 1 cut(s) 389
XmiI GTMKAC 1 cut(s) 516
XmnI GAANNNNTTC 1 cut(s) 859
XspI CTAG 3 cut(s) 608, 647, 1190
ZraI GACGTC 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.