FvH4_1g09580

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014883
Physical Location & Seq
Reverse (-)
5138334 .. 5140484
2151 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g09580.t1

Sequence Viewer

Length: 1638 bp
ATGGCTTCCTCCAGCCCAAAAGTGCTTTTGCAGCTTCTGCTTGTCTGTATGATCTTCTTCTCAACAACTAAGGCTCTCAACAATTCTTCTTCTTCATCATCAACAACTAATACTCTCAATCCCCATTTGTCTTCCATTAAGTCCTTCTGCAAGTCCACACCTTACCCTGATGTCTGCTTCGACTCACTGAAGCTCTCAATCTCCATCAACATCTCCCCCAACATCCTCACTTACCTCCTCCAATCCCTCCAAATCGCGCTATCTGAAGCCACAAAGCTCTCCGATCTCTTCTACAAGGCGGGACTATACTCGAACATAGTTGAGAAACAAAAGGGAGCAATCCAGGACTGCAAAGACCTCCATCAGATCACACTTTCTTCCATACGGCGATCAGTTTCTGGAGTGAAGTCATCGAACAGCAAGAAACTAAGGGATGCAAAAGCCTATCTCAGTGCTGCCCTCACAAACAAGAACACTTGCTTGGAAGGCTTGGATTCTGCTTCTGGTCCAATGAAGCCAACCCTGGTCAGTTCCTTAACTAGTACATACAAGTATGTTAGCAACTCTCTTTCTGCTCTCAAGAGTCAAGTGCCTAGTCCAAAAGGCCGTAAAAACCGGCGTCTGTTGGGAGTTCCTGCATGGATTTCTAGGAGAGATCGCCGGATTTTGGAGAGCTCCGGTGACGAATATGACCCGAGTGAAGTGTTGACAGTATCTGCTGACGGTAGTGGGAACTTCACCACCATCAGTGATGCTGTTAATTTTGCTCCAAATAATAGTTTTGACAGGATTATTATCTATGTCAAAAAAGGGTTTTATGATGAAAATGTGGAAATCCCAAGCTGGAAGACGAACATTGTTCTTCTTGGAGATGGAAGTGACATCACTGTCATCAGTGGCATCAGAAGTGTCGCTGATGGTTGGACCACTTTCAGATCTGCAACACTAGCTGTATCCGGGGAAGGCTTTCTGGCAAGAGACATAGGCATTGAAAACAGAGCAGGACCAGAGAAGCACCAAGCAGTTGCATTGAGAGTAAATGCAGACCTTGCTGCATTGTACAAGTGTGCCTTCAGCGGTTACCAAGACACGATGTACGTGCACTCTTTCAGACAGTTTTACAGAGAGTGTGACATCTCAGGGACCATCGACTACATATTTGGTAACGCCGCTGTTGTAATCCAAGACTGCAACATTGTATCCAAGATGCCAATGCCGGGACAGTTCACCGTTATCACAGCACAAGCCAGGGACAGCGAAGAGGAGGATACCGGAATCTCAATACAGAATTGTTCAGTCGTGGCTGCTGATGACTTGTACGCCAACAAAGGCAGTGTCAAAAGTTACTTAGGCCGGCCGTGGAGGGTGTACTCTAGGACAGTTTTCCTAGAGTCCTACATTGATGACTTCATTGACCCGAGTGGGTGGAAAGATTGGTCTGGGGATCTAGGCTTAGACACTTTGTACTATGGAGAGTATGAAAATTATGGACCTGGTTCAGGGACGGATAGTAGAGTAAACTGGACAGGGTATCATATTATGGACTACTATGATGCCTCAAATTTCTCAGTATCAGAGTTCATTGTAGGTGATGAATGGCTGCAGTACACTTCATTCCCTTATGATGATGGCATCTGA

Protein Analysis

546

Amino Acids

60.03

Weight (kDa)

5.47

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 44 - 191 3.6e-25 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 237 - 531 5.9e-136 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014883)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26440
fragaria_vesca FvH4_1g09580
malus_domestica MD02G1104400.v1.1 MD02G1104500.v1.1 MD15G1224800.v1.1
prunus_persica Prupe.7G190400_v2.0.a1
pyrus_communis pycom02g08230
rosa_chinensis RchiOBHm_Chr2g0096441
rosa_laevigata RLG00000016640
rosa_multiflora Rmu_sc0004096.1_g000025
rosa_roxburghii Rroxscaffold_2G00145650
rosa_samantha Rh2AG104700 Rh2BG106900 Rh2CG108700 Rh2DG107800
rosa_wichuraiana Rw2G008090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 887
AccII CGCG 1 cut(s) 257
AciI CCGC 3 cut(s) 299, 1077, 1170
AclWI GGATC 1 cut(s) 1452
AcoI YGGCCR 1 cut(s) 1355
AcsI RAATTY 1 cut(s) 1561
AcuI CTGAAG 3 cut(s) 209, 285, 1057
AcyI GRCGYC 1 cut(s) 619
AfaI GTAC 7 cut(s) 544, 1061, 1097, 1319, 1370, 1466, 1607
AfiI CCNNNNNNNGG 3 cut(s) 667, 1217, 1499
AgsI TTSAA 1 cut(s) 992
AhlI ACTAGT 1 cut(s) 539
AjnI CCWGG 4 cut(s) 342, 522, 1247, 1492
AjuI GAANNNNNNNTTGG 2 cut(s) 464, 496
AluBI AGCT 6 cut(s) 34, 193, 277, 675, 843, 950
AluI AGCT 6 cut(s) 34, 193, 277, 675, 843, 950
Alw21I GWGCWC 2 cut(s) 677, 1104
Alw26I GTCTC 1 cut(s) 972
Alw44I GTGCAC 1 cut(s) 1100
AlwI GGATC 1 cut(s) 1452
AlwNI CAGNNNCTG 3 cut(s) 37, 398, 716
Ama87I CYCGRG 2 cut(s) 694, 1417
AoxI GGCC 3 cut(s) 604, 1351, 1355
ApaLI GTGCAC 1 cut(s) 1100
ApeKI GCWGC 5 cut(s) 31, 455, 1052, 1304, 1600
ApoI RAATTY 1 cut(s) 1561
ArsI GACNNNNNNTTYG 4 cut(s) 1142, 1174, 1320, 1352
Asp700I GAANNNNTTC 1 cut(s) 966
AspLEI GCGC 1 cut(s) 259
AspS9I GGNCC 5 cut(s) 506, 924, 1004, 1143, 1490
AsuC2I CCSGG 2 cut(s) 958, 1218
AsuHPI GGTGA 4 cut(s) 692, 730, 1219, 1601
AvaI CYCGRG 2 cut(s) 694, 1417
AvaII GGWCC 5 cut(s) 506, 924, 1004, 1143, 1490
BaeGI GKGCMC 1 cut(s) 1104
BanII GRGCYC 1 cut(s) 677
BbsI GAAGAC 2 cut(s) 123, 854
Bbv12I GWGCWC 2 cut(s) 677, 1104
BbvI GCAGC 5 cut(s) 43, 442, 1039, 1291, 1587
BccI CCATC 7 cut(s) 212, 369, 752, 866, 911, 1154, 1622
BceAI ACGGC 3 cut(s) 401, 591, 1342
BcgI CGANNNNNNTGC 2 cut(s) 1081, 1115
BciT130I CCWGG 4 cut(s) 344, 524, 1249, 1494
BciVI GTATCC 3 cut(s) 964, 1210, 1261
BcnI CCSGG 2 cut(s) 958, 1218
BcoDI GTCTC 1 cut(s) 972
BcuI ACTAGT 1 cut(s) 539
BfaI CTAG 7 cut(s) 540, 594, 648, 947, 1374, 1388, 1448
BfmI CTRYAG 1 cut(s) 1601
BfuI GTATCC 3 cut(s) 964, 1210, 1261
BglII AGATCT 1 cut(s) 935
BisI GCNGC 6 cut(s) 32, 456, 1053, 1170, 1305, 1601
BlsI GCNGC 6 cut(s) 33, 457, 1054, 1171, 1306, 1602
Bme1390I CCNGG 6 cut(s) 344, 524, 958, 1218, 1249, 1494
Bme18I GGWCC 5 cut(s) 506, 924, 1004, 1143, 1490
BmeT110I CYCGRG 2 cut(s) 694, 1417
BmgT120I GGNCC 5 cut(s) 506, 924, 1004, 1143, 1490
BmiI GGNNCC 1 cut(s) 1144
BmrFI CCNGG 6 cut(s) 344, 524, 958, 1218, 1249, 1494
BmsI GCATC 5 cut(s) 424, 742, 909, 1197, 1543
BpiI GAAGAC 2 cut(s) 123, 854
BpmI CTGGAG 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 563
BpuMI CCSGG 2 cut(s) 958, 1218
BsaAI YACGTR 1 cut(s) 1099
BsaBI GATNNNNATC 1 cut(s) 794
BsaHI GRCGYC 1 cut(s) 619
BsaJI CCNNGG 4 cut(s) 522, 957, 1248, 1358
BsaWI WCCGGW 2 cut(s) 677, 1271
Bsc4I CCNNNNNNNGG 3 cut(s) 667, 1217, 1499
Bse118I RCCGGY 2 cut(s) 615, 1353
Bse1I ACTGG 1 cut(s) 1526
Bse8I GATNNNNATC 1 cut(s) 794
BseBI CCWGG 4 cut(s) 344, 524, 1249, 1494
BseDI CCNNGG 4 cut(s) 522, 957, 1248, 1358
BseGI GGATG 2 cut(s) 222, 439
BseJI GATNNNNATC 1 cut(s) 794
BseLI CCNNNNNNNGG 3 cut(s) 667, 1217, 1499
BseMII CTCAG 3 cut(s) 463, 1152, 1581
BseNI ACTGG 1 cut(s) 1526
BseRI GAGGAG 2 cut(s) 227, 1277
BseSI GKGCMC 1 cut(s) 1104
BseX3I CGGCCG 1 cut(s) 1355
BseXI GCAGC 5 cut(s) 43, 442, 1039, 1291, 1587
Bsh1236I CGCG 1 cut(s) 257
Bsh1285I CGRYCG 1 cut(s) 1358
BshFI GGCC 3 cut(s) 606, 1353, 1357
BsiEI CGRYCG 1 cut(s) 1358
BsiHKAI GWGCWC 2 cut(s) 677, 1104
BsiHKCI CYCGRG 2 cut(s) 694, 1417
BsiSI CCGG 7 cut(s) 616, 661, 678, 957, 1217, 1272, 1354
BslFI GGGAC 5 cut(s) 315, 1156, 1233, 1265, 1516
BslI CCNNNNNNNGG 3 cut(s) 667, 1217, 1499
BsmAI GTCTC 1 cut(s) 972
BsmFI GGGAC 5 cut(s) 315, 1156, 1233, 1265, 1516
BsnI GGCC 3 cut(s) 606, 1353, 1357
BsoBI CYCGRG 2 cut(s) 694, 1417
Bsp1286I GDGCHC 2 cut(s) 677, 1104
Bsp1407I TGTACA 1 cut(s) 1059
Bsp143I GATC 7 cut(s) 51, 283, 366, 389, 655, 935, 1444
BspACI CCGC 3 cut(s) 299, 1077, 1170
BspANI GGCC 3 cut(s) 606, 1353, 1357
BspCNI CTCAG 3 cut(s) 462, 1151, 1580
BspFNI CGCG 1 cut(s) 257
BspLI GGNNCC 1 cut(s) 1144
BspMAI CTGCAG 1 cut(s) 1605
BspPI GGATC 1 cut(s) 1452
BsrFI RCCGGY 2 cut(s) 615, 1353
BsrGI TGTACA 1 cut(s) 1059
BsrI ACTGG 1 cut(s) 1526
BssAI RCCGGY 2 cut(s) 615, 1353
BssECI CCNNGG 4 cut(s) 522, 957, 1248, 1358
BssMI GATC 7 cut(s) 51, 283, 366, 389, 655, 935, 1444
BssNI GRCGYC 1 cut(s) 619
Bst2UI CCWGG 4 cut(s) 344, 524, 1249, 1494
Bst4CI ACNGT 7 cut(s) 712, 725, 889, 1116, 1224, 1231, 1381
Bst6I CTCTTC 2 cut(s) 293, 1254
BstACI GRCGYC 1 cut(s) 619
BstAPI GCANNNNNTGC 2 cut(s) 37, 1049
BstAUI TGTACA 1 cut(s) 1059
BstBAI YACGTR 1 cut(s) 1099
BstC8I GCNNGC 1 cut(s) 1355
BstDEI CTNAG 7 cut(s) 69, 428, 449, 1138, 1348, 1453, 1567
BstDSI CCRYGG 1 cut(s) 1358
BstEII GGTNACC 1 cut(s) 1079
BstENI CCTNNNNNAGG 1 cut(s) 1497
BstF5I GGATG 2 cut(s) 222, 439
BstFNI CGCG 1 cut(s) 257
BstHHI GCGC 1 cut(s) 259
BstKTI GATC 7 cut(s) 54, 286, 369, 392, 658, 938, 1447
BstMAI GTCTC 1 cut(s) 972
BstMBI GATC 7 cut(s) 51, 283, 366, 389, 655, 935, 1444
BstMCI CGRYCG 1 cut(s) 1358
BstMWI GCNNNNNNNGC 5 cut(s) 31, 37, 486, 947, 1049
BstNI CCWGG 4 cut(s) 344, 524, 1249, 1494
BstPI GGTNACC 1 cut(s) 1079
BstSCI CCNGG 6 cut(s) 342, 522, 956, 1216, 1247, 1492
BstSFI CTRYAG 1 cut(s) 1601
BstSLI GKGCMC 1 cut(s) 1104
BstUI CGCG 1 cut(s) 257
BstV1I GCAGC 5 cut(s) 43, 442, 1039, 1291, 1587
BstV2I GAAGAC 2 cut(s) 123, 854
BstX2I RGATCY 2 cut(s) 935, 1444
BstYI RGATCY 2 cut(s) 935, 1444
BstZI CGGCCG 1 cut(s) 1355
BsuI GTATCC 3 cut(s) 964, 1210, 1261
BsuRI GGCC 3 cut(s) 606, 1353, 1357
BtgI CCRYGG 1 cut(s) 1358
BtsCI GGATG 2 cut(s) 222, 439
BtsI GCAGTG 1 cut(s) 1339
BtsIMutI CAGTG 6 cut(s) 185, 457, 754, 885, 901, 1339
Cac8I GCNNGC 1 cut(s) 1355
CaiI CAGNNNCTG 3 cut(s) 37, 398, 716
CfoI GCGC 1 cut(s) 259
Cfr10I RCCGGY 2 cut(s) 615, 1353
Cfr13I GGNCC 5 cut(s) 506, 924, 1004, 1143, 1490
CseI GACGC 1 cut(s) 608
CsiI ACCWGGT 1 cut(s) 1492
Csp6I GTAC 7 cut(s) 543, 1060, 1096, 1318, 1369, 1465, 1606
CviAII CATG 1 cut(s) 639
CviQI GTAC 7 cut(s) 543, 1060, 1096, 1318, 1369, 1465, 1606
DdeI CTNAG 7 cut(s) 69, 428, 449, 1138, 1348, 1453, 1567
DpnI GATC 7 cut(s) 53, 285, 368, 391, 657, 937, 1446
DpnII GATC 7 cut(s) 51, 283, 366, 389, 655, 935, 1444
DrdI GACNNNNNNGTC 1 cut(s) 887
DseDI GACNNNNNNGTC 1 cut(s) 887
EaeI YGGCCR 1 cut(s) 1355
EagI CGGCCG 1 cut(s) 1355
Eam1104I CTCTTC 2 cut(s) 293, 1254
EarI CTCTTC 2 cut(s) 293, 1254
Ecl136II GAGCTC 1 cut(s) 675
EclXI CGGCCG 1 cut(s) 1355
Eco24I GRGCYC 1 cut(s) 677
Eco47I GGWCC 5 cut(s) 506, 924, 1004, 1143, 1490
Eco52I CGGCCG 1 cut(s) 1355
Eco53kI GAGCTC 1 cut(s) 675
Eco57I CTGAAG 3 cut(s) 209, 285, 1057
Eco88I CYCGRG 2 cut(s) 694, 1417
Eco91I GGTNACC 1 cut(s) 1079
EcoICRI GAGCTC 1 cut(s) 675
EcoNI CCTNNNNNAGG 1 cut(s) 1497
EcoO65I GGTNACC 1 cut(s) 1079
EcoRII CCWGG 4 cut(s) 342, 522, 1247, 1492
EcoT38I GRGCYC 1 cut(s) 677
FaeI CATG 1 cut(s) 642
FalI AAGNNNNNCTT 2 cut(s) 1055, 1087
FaqI GGGAC 5 cut(s) 315, 1156, 1233, 1265, 1516
FatI CATG 1 cut(s) 638
FauI CCCGC 1 cut(s) 292
Fnu4HI GCNGC 6 cut(s) 32, 456, 1053, 1170, 1305, 1601
FokI GGATG 2 cut(s) 209, 446
FriOI GRGCYC 1 cut(s) 677
FseI GGCCGGCC 1 cut(s) 1357
Fsp4HI GCNGC 6 cut(s) 32, 456, 1053, 1170, 1305, 1601
FspBI CTAG 7 cut(s) 540, 594, 648, 947, 1374, 1388, 1448
GlaI GCGC 1 cut(s) 258
GluI GCNGC 6 cut(s) 32, 456, 1053, 1170, 1305, 1601
GsuI CTGGAG 1 cut(s) 420
HaeIII GGCC 3 cut(s) 606, 1353, 1357
HapII CCGG 7 cut(s) 616, 661, 678, 957, 1217, 1272, 1354
HgaI GACGC 1 cut(s) 608
HhaI GCGC 1 cut(s) 259
Hin1I GRCGYC 1 cut(s) 619
Hin1II CATG 1 cut(s) 642
Hin6I GCGC 1 cut(s) 257
HinP1I GCGC 1 cut(s) 257
HincII GTYRAC 1 cut(s) 708
HindII GTYRAC 1 cut(s) 708
HinfI GANTC 5 cut(s) 182, 494, 583, 1275, 1391
HpaII CCGG 7 cut(s) 616, 661, 678, 957, 1217, 1272, 1354
HphI GGTGA 4 cut(s) 692, 730, 1219, 1601
Hpy166II GTNNAC 7 cut(s) 156, 708, 1102, 1227, 1369, 1519, 1608
Hpy188I TCNGA 8 cut(s) 265, 283, 366, 905, 935, 1112, 1576, 1637
Hpy188III TCNNGA 2 cut(s) 399, 580
Hpy8I GTNNAC 7 cut(s) 156, 708, 1102, 1227, 1369, 1519, 1608
HpyAV CCTTC 4 cut(s) 154, 479, 956, 1081
HpyCH4III ACNGT 7 cut(s) 712, 725, 889, 1116, 1224, 1231, 1381
HpyCH4IV ACGT 1 cut(s) 1098
HpyF10VI GCNNNNNNNGC 5 cut(s) 31, 37, 486, 947, 1049
HpyF3I CTNAG 7 cut(s) 69, 428, 449, 1138, 1348, 1453, 1567
HpySE526I ACGT 1 cut(s) 1098
Hsp92I GRCGYC 1 cut(s) 619
Hsp92II CATG 1 cut(s) 642
HspAI GCGC 1 cut(s) 257
KroI GCCGGC 1 cut(s) 1353
KroNI GCCGGC 1 cut(s) 1355
Kzo9I GATC 7 cut(s) 51, 283, 366, 389, 655, 935, 1444
LmnI GCTCC 3 cut(s) 335, 680, 772
Lsp1109I GCAGC 5 cut(s) 43, 442, 1039, 1291, 1587
LweI GCATC 5 cut(s) 424, 742, 909, 1197, 1543
MabI ACCWGGT 1 cut(s) 1492
MaeI CTAG 7 cut(s) 540, 594, 648, 947, 1374, 1388, 1448
MaeII ACGT 1 cut(s) 1098
MaeIII GTNAC 6 cut(s) 680, 878, 1079, 1130, 1163, 1343
MalI GATC 7 cut(s) 53, 285, 368, 391, 657, 937, 1446
MboI GATC 7 cut(s) 51, 283, 366, 389, 655, 935, 1444
MflI RGATCY 2 cut(s) 935, 1444
MhlI GDGCHC 2 cut(s) 677, 1104
MluCI AATT 5 cut(s) 82, 760, 1288, 1483, 1561
MlyI GAGTC 3 cut(s) 176, 592, 1400
MmeI TCCRAC 1 cut(s) 902
MroNI GCCGGC 1 cut(s) 1353
MroXI GAANNNNTTC 1 cut(s) 966
MseI TTAA 3 cut(s) 138, 536, 759
MspA1I CMGCKG 2 cut(s) 1077, 1172
MspI CCGG 7 cut(s) 616, 661, 678, 957, 1217, 1272, 1354
MspR9I CCNGG 6 cut(s) 344, 524, 958, 1218, 1249, 1494
MvaI CCWGG 4 cut(s) 344, 524, 1249, 1494
MvnI CGCG 1 cut(s) 257
MwoI GCNNNNNNNGC 5 cut(s) 31, 37, 486, 947, 1049
NaeI GCCGGC 1 cut(s) 1355
NciI CCSGG 2 cut(s) 958, 1218
NdeII GATC 7 cut(s) 51, 283, 366, 389, 655, 935, 1444
NgoMIV GCCGGC 1 cut(s) 1353
NlaIII CATG 1 cut(s) 642
NlaIV GGNNCC 1 cut(s) 1144
NmuCI GTSAC 3 cut(s) 680, 878, 1130
PdiI GCCGGC 1 cut(s) 1355
PdmI GAANNNNTTC 1 cut(s) 966
PfeI GAWTC 2 cut(s) 494, 1275
PfoI TCCNGGA 1 cut(s) 342
PkrI GCNGC 6 cut(s) 33, 457, 1054, 1171, 1306, 1602
PleI GAGTC 3 cut(s) 176, 591, 1399
PpsI GAGTC 3 cut(s) 176, 591, 1399
Ppu21I YACGTR 1 cut(s) 1099
Psp124BI GAGCTC 1 cut(s) 677
Psp6I CCWGG 4 cut(s) 342, 522, 1247, 1492
PspEI GGTNACC 1 cut(s) 1079
PspGI CCWGG 4 cut(s) 342, 522, 1247, 1492
PspN4I GGNNCC 1 cut(s) 1144
PspPI GGNCC 5 cut(s) 506, 924, 1004, 1143, 1490
PstI CTGCAG 1 cut(s) 1605
PstNI CAGNNNCTG 3 cut(s) 37, 398, 716
PsuI RGATCY 2 cut(s) 935, 1444
RigI GGCCGGCC 1 cut(s) 1357
RsaI GTAC 7 cut(s) 544, 1061, 1097, 1319, 1370, 1466, 1607
RsaNI GTAC 7 cut(s) 543, 1060, 1096, 1318, 1369, 1465, 1606
SacI GAGCTC 1 cut(s) 677
SaqAI TTAA 3 cut(s) 138, 536, 759
SatI GCNGC 6 cut(s) 32, 456, 1053, 1170, 1305, 1601
Sau3AI GATC 7 cut(s) 51, 283, 366, 389, 655, 935, 1444
Sau96I GGNCC 5 cut(s) 506, 924, 1004, 1143, 1490
SchI GAGTC 3 cut(s) 176, 592, 1400
ScrFI CCNGG 6 cut(s) 344, 524, 958, 1218, 1249, 1494
SduI GDGCHC 2 cut(s) 677, 1104
SexAI ACCWGGT 1 cut(s) 1492
SfaNI GCATC 5 cut(s) 424, 742, 909, 1197, 1543
SfcI CTRYAG 1 cut(s) 1601
SinI GGWCC 5 cut(s) 506, 924, 1004, 1143, 1490
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
SpeI ACTAGT 1 cut(s) 539
Sse9I AATT 5 cut(s) 82, 760, 1288, 1483, 1561
SsiI CCGC 3 cut(s) 299, 1077, 1170
SspMI CTAG 7 cut(s) 540, 594, 648, 947, 1374, 1388, 1448
SstI GAGCTC 1 cut(s) 677
StyD4I CCNGG 6 cut(s) 342, 522, 956, 1216, 1247, 1492
TaaI ACNGT 7 cut(s) 712, 725, 889, 1116, 1224, 1231, 1381
TaiI ACGT 1 cut(s) 1101
TaqI TCGA 4 cut(s) 180, 311, 413, 1149
TasI AATT 5 cut(s) 82, 760, 1288, 1483, 1561
TatI WGTACW 5 cut(s) 542, 1059, 1368, 1464, 1605
TauI GCSGC 1 cut(s) 1172
TfiI GAWTC 2 cut(s) 494, 1275
Tru1I TTAA 3 cut(s) 138, 536, 759
Tru9I TTAA 3 cut(s) 138, 536, 759
TscAI CASTG 6 cut(s) 192, 457, 754, 892, 901, 1339
TseFI GTSAC 3 cut(s) 680, 878, 1130
TseI GCWGC 5 cut(s) 31, 455, 1052, 1304, 1600
Tsp45I GTSAC 3 cut(s) 680, 878, 1130
TspDTI ATGAA 8 cut(s) 84, 527, 837, 1399, 1494, 1570, 1602, 1608
TspGWI ACGGA 1 cut(s) 1520
TspRI CASTG 6 cut(s) 192, 457, 754, 892, 901, 1339
VneI GTGCAC 1 cut(s) 1100
VpaK11BI GGWCC 5 cut(s) 506, 924, 1004, 1143, 1490
XagI CCTNNNNNAGG 1 cut(s) 1497
XapI RAATTY 1 cut(s) 1561
XmnI GAANNNNTTC 1 cut(s) 966
XspI CTAG 7 cut(s) 540, 594, 648, 947, 1374, 1388, 1448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.