Rh2AG104700

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0014883
Physical Location & Seq
Reverse (-)
9147758 .. 9150049
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG104700.1

Sequence Viewer

Length: 1650 bp
ATGGCTTCCTCCATCCCAAAAGTGCTTTTGCAGTTTTTGCTTCTTTGTATAATCTTCTTCTCAACAACTAAGGCTCTCTGCAATTCTTCTTCTTCTTCTTCATCAACAACTAATACTCTCAATCCCCATTTGTCTTCCATTAAATCCTTCTGCAAGTCCACTCCCTACCCCGATGTCTGTTTCGACTCGCTGAAGCTCTCAATCTCCATCAACATCTCCCCCAACATCCTCACTTACCTCCTCCAATCCCTCCAAATCGCGCTATCCGAAGCCACAAAGCTCTCCGATCTTTTCTACAAGGCAGGACTTTACTCCAACATTGTTGAGAAACAAAAGGGAGCAATCCAGGACTGCAAAGACCTCCACCAGATCACACTCTCTTCCATAAAACGATCAGTTTCTGGAGTCAAGTCTTCGAACAACAAGAAACTAAGGGATGCAAGGGCCTATCTCAGTGCTGCCCTCACAAACAAGAACACATGCTTGGAAGGATTGGAGTCTGCTTCTGGTCCTATGAAGCCAACCCTGGTCAGCTCCTTAACTAGTACATACAAGTATGTTAGCAACTCTCTTTCTGCTCTCAAGAGTCAAGTGCCTTATCCAAAAGGCCGTAAAAACCGGCGTCTGTTGGGAGTTCCGACATGGATTTCTCGGAGAGATCGCCGGATTCTGGAGAGCTCGGGTGAAGAATATGACCCGAGTGAAATGCTGACAGTATCTGCAGATGGTAGTGGGAACTTCACCACCATCAGTGGTGCTGTTAATTTTGCTCCAAATAATAGTTACGACAGGACTATCATCTATGTCAAAGAAGGGGTTTATGATGAAAATGTGGAAATCCCAAGCTGGAAGACAAACATTGTTCTTCTTGGAGATGGAAGTGGCATCACTGTCATCACTGGTAACAGAAGCAATGCTGATGGTTGGACCACTTTCAGATCTGCAACATTAGCTGTATCTGGGGAAGGCTTTCTGGCAAGAGACATAAGTATTGAAAACAGAGCAGGACCAGAGAAGCACCAAGCAGTTGCATTGAGAGTAAATGCAGACCTTGCTGCATTGTACAAGTGCGCCTTCAGCGGTTACCAAGACACAATGTATGTGCACTCTTTCAGACAGTTTTATAGAGAGTGTGATATCTCAGGGACCATAGACTACATATTTGGTAACGCCGCTGTGGTAATCCAAGATTGCAACATTATATCCAAGATGCCAATGCCGGGACAGTTCACCGTTATCACAGCACAAGCCAGGGACTCCGAAGAGGAGGATACCGGAATCTCAATACAGAATTGTTCAATTGTGGCTGCTGATGACTTGTACGCCAACAAGGACAGTGTCAAAAGTTACTTAGGCCGGCCGTGGAGGGTATACTCTAGGACGGTTTTCCTAGAGTCCTACATTGATGACTTCATTGACCCCAGTGGGTGGACAAATTGGTCTGGGGACCTAGGCTTGGACACTTTGTACTATGGAGAGTTTGAAAATTATGGACCTGGTTCAGGGACGGAGAGTAGAGTAAACTGGACAGGGTATCATATTATGGATTACTATGATGCATCAAATTTCACAGTATCAGAGTTCATTGCAGGTGATGAATGGCTGCAGTCCACTTCATTTCCTTATGATGATGGGATTGATGGCATCTGA

Protein Analysis

549

Amino Acids

60.51

Weight (kDa)

5.3

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 45 - 192 2.3e-25 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 238 - 532 8.3e-137 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014883)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26440
fragaria_vesca FvH4_1g09580
malus_domestica MD02G1104400.v1.1 MD02G1104500.v1.1 MD15G1224800.v1.1
prunus_persica Prupe.7G190400_v2.0.a1
pyrus_communis pycom02g08230
rosa_chinensis RchiOBHm_Chr2g0096441
rosa_laevigata RLG00000016640
rosa_multiflora Rmu_sc0004096.1_g000025
rosa_roxburghii Rroxscaffold_2G00145650
rosa_samantha Rh2AG104700 Rh2BG106900 Rh2CG108700 Rh2DG107800
rosa_wichuraiana Rw2G008090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1580
AasI GACNNNNNNGTC 1 cut(s) 1438
Acc36I ACCTGC 1 cut(s) 1580
AccB7I CCANNNNNTGG 1 cut(s) 1428
AccI GTMKAC 1 cut(s) 1371
AccII CGCG 1 cut(s) 260
AciI CCGC 2 cut(s) 1080, 1173
AcoI YGGCCR 1 cut(s) 1358
AcsI RAATTY 1 cut(s) 1564
AcuI CTGAAG 2 cut(s) 212, 1060
AcyI GRCGYC 1 cut(s) 622
AfaI GTAC 4 cut(s) 547, 1064, 1322, 1469
AfiI CCNNNNNNNGG 5 cut(s) 670, 1220, 1428, 1456, 1502
AgsI TTSAA 3 cut(s) 995, 1299, 1484
AhlI ACTAGT 1 cut(s) 542
AjnI CCWGG 4 cut(s) 345, 525, 1250, 1495
AjuI GAANNNNNNNTTGG 2 cut(s) 467, 499
AluBI AGCT 6 cut(s) 196, 280, 534, 678, 846, 953
AluI AGCT 6 cut(s) 196, 280, 534, 678, 846, 953
Alw21I GWGCWC 2 cut(s) 680, 1107
Alw26I GTCTC 1 cut(s) 975
Alw44I GTGCAC 1 cut(s) 1103
AlwNI CAGNNNCTG 2 cut(s) 401, 719
Ama87I CYCGRG 2 cut(s) 679, 697
AoxI GGCC 4 cut(s) 444, 607, 1354, 1358
ApaLI GTGCAC 1 cut(s) 1103
ApeKI GCWGC 4 cut(s) 458, 1055, 1307, 1603
ApoI RAATTY 1 cut(s) 1564
ArsI GACNNNNNNTTYG 2 cut(s) 1145, 1177
Asp700I GAANNNNTTC 1 cut(s) 969
AspA2I CCTAGG 1 cut(s) 1450
AspLEI GCGC 2 cut(s) 262, 1073
AspS9I GGNCC 7 cut(s) 444, 509, 927, 1007, 1146, 1447, 1493
AsuC2I CCSGG 1 cut(s) 1221
AsuHPI GGTGA 4 cut(s) 695, 733, 1222, 1604
AsuII TTCGAA 1 cut(s) 416
AvaI CYCGRG 2 cut(s) 679, 697
AvaII GGWCC 6 cut(s) 509, 927, 1007, 1146, 1447, 1493
AvrII CCTAGG 1 cut(s) 1450
BaeGI GKGCMC 1 cut(s) 1107
BanII GRGCYC 1 cut(s) 680
BbsI GAAGAC 3 cut(s) 126, 405, 857
Bbv12I GWGCWC 2 cut(s) 680, 1107
BbvI GCAGC 4 cut(s) 445, 1042, 1294, 1590
BccI CCATC 8 cut(s) 20, 215, 719, 755, 869, 914, 1625, 1634
BceAI ACGGC 2 cut(s) 594, 1345
BcgI CGANNNNNNTGC 2 cut(s) 688, 722
BciT130I CCWGG 4 cut(s) 347, 527, 1252, 1497
BciVI GTATCC 1 cut(s) 1264
BcnI CCSGG 1 cut(s) 1221
BcoDI GTCTC 1 cut(s) 975
BcuI ACTAGT 1 cut(s) 542
BfaI CTAG 4 cut(s) 543, 1377, 1391, 1451
BfmI CTRYAG 2 cut(s) 720, 1604
BfuAI ACCTGC 1 cut(s) 1580
BfuI GTATCC 1 cut(s) 1264
BglII AGATCT 1 cut(s) 938
BisI GCNGC 5 cut(s) 459, 1056, 1173, 1308, 1604
BlnI CCTAGG 1 cut(s) 1450
BlsI GCNGC 5 cut(s) 460, 1057, 1174, 1309, 1605
Bme1390I CCNGG 5 cut(s) 347, 527, 1221, 1252, 1497
Bme18I GGWCC 6 cut(s) 509, 927, 1007, 1146, 1447, 1493
BmeT110I CYCGRG 2 cut(s) 679, 697
BmgT120I GGNCC 7 cut(s) 444, 509, 927, 1007, 1146, 1447, 1493
BmiI GGNNCC 2 cut(s) 1147, 1448
BmrFI CCNGG 5 cut(s) 347, 527, 1221, 1252, 1497
BmrI ACTGGG 1 cut(s) 1416
BmsI GCATC 5 cut(s) 427, 894, 1200, 1546, 1568
BmuI ACTGGG 1 cut(s) 1416
BpiI GAAGAC 3 cut(s) 126, 405, 857
BpmI CTGGAG 2 cut(s) 423, 692
Bpu14I TTCGAA 1 cut(s) 416
BpuEI CTTGAG 1 cut(s) 566
BpuMI CCSGG 1 cut(s) 1221
BsaHI GRCGYC 1 cut(s) 622
BsaJI CCNNGG 4 cut(s) 525, 1251, 1361, 1450
BsaWI WCCGGW 1 cut(s) 1274
Bsc4I CCNNNNNNNGG 5 cut(s) 670, 1220, 1428, 1456, 1502
Bse118I RCCGGY 2 cut(s) 618, 1356
Bse1I ACTGG 3 cut(s) 904, 1422, 1529
Bse3DI GCAATG 2 cut(s) 919, 1584
BseBI CCWGG 4 cut(s) 347, 527, 1252, 1497
BseDI CCNNGG 4 cut(s) 525, 1251, 1361, 1450
BseGI GGATG 3 cut(s) 12, 225, 442
BseLI CCNNNNNNNGG 5 cut(s) 670, 1220, 1428, 1456, 1502
BseMI GCAATG 2 cut(s) 919, 1584
BseMII CTCAG 2 cut(s) 466, 1155
BseNI ACTGG 3 cut(s) 904, 1422, 1529
BseRI GAGGAG 2 cut(s) 230, 1280
BseSI GKGCMC 1 cut(s) 1107
BseX3I CGGCCG 1 cut(s) 1358
BseXI GCAGC 4 cut(s) 445, 1042, 1294, 1590
Bsh1236I CGCG 1 cut(s) 260
Bsh1285I CGRYCG 1 cut(s) 1361
BshFI GGCC 4 cut(s) 446, 609, 1356, 1360
BsiEI CGRYCG 1 cut(s) 1361
BsiHKAI GWGCWC 2 cut(s) 680, 1107
BsiHKCI CYCGRG 2 cut(s) 679, 697
BsiSI CCGG 5 cut(s) 619, 664, 1220, 1275, 1357
BslFI GGGAC 5 cut(s) 1159, 1236, 1268, 1460, 1519
BslI CCNNNNNNNGG 5 cut(s) 670, 1220, 1428, 1456, 1502
BsmAI GTCTC 1 cut(s) 975
BsmFI GGGAC 5 cut(s) 1159, 1236, 1268, 1460, 1519
BsnI GGCC 4 cut(s) 446, 609, 1356, 1360
BsoBI CYCGRG 2 cut(s) 679, 697
Bsp119I TTCGAA 1 cut(s) 416
Bsp1286I GDGCHC 2 cut(s) 680, 1107
Bsp1407I TGTACA 1 cut(s) 1062
Bsp143I GATC 5 cut(s) 286, 369, 392, 658, 938
BspACI CCGC 2 cut(s) 1080, 1173
BspANI GGCC 4 cut(s) 446, 609, 1356, 1360
BspCNI CTCAG 2 cut(s) 465, 1154
BspFNI CGCG 1 cut(s) 260
BspLI GGNNCC 2 cut(s) 1147, 1448
BspMAI CTGCAG 2 cut(s) 724, 1608
BspMI ACCTGC 1 cut(s) 1580
BspT104I TTCGAA 1 cut(s) 416
BsrDI GCAATG 2 cut(s) 919, 1584
BsrFI RCCGGY 2 cut(s) 618, 1356
BsrGI TGTACA 1 cut(s) 1062
BsrI ACTGG 3 cut(s) 904, 1422, 1529
BssAI RCCGGY 2 cut(s) 618, 1356
BssECI CCNNGG 4 cut(s) 525, 1251, 1361, 1450
BssMI GATC 5 cut(s) 286, 369, 392, 658, 938
BssNAI GTATAC 1 cut(s) 1372
BssNI GRCGYC 1 cut(s) 622
BssT1I CCWWGG 1 cut(s) 1450
Bst1107I GTATAC 1 cut(s) 1372
Bst2UI CCWGG 4 cut(s) 347, 527, 1252, 1497
Bst4CI ACNGT 8 cut(s) 715, 892, 1119, 1227, 1234, 1337, 1384, 1573
Bst6I CTCTTC 2 cut(s) 385, 1257
BstACI GRCGYC 1 cut(s) 622
BstAPI GCANNNNNTGC 2 cut(s) 37, 1052
BstAUI TGTACA 1 cut(s) 1062
BstBI TTCGAA 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 1358
BstDEI CTNAG 5 cut(s) 69, 431, 452, 1141, 1351
BstDSI CCRYGG 1 cut(s) 1361
BstEII GGTNACC 1 cut(s) 1082
BstENI CCTNNNNNAGG 1 cut(s) 1500
BstF5I GGATG 3 cut(s) 12, 225, 442
BstFNI CGCG 1 cut(s) 260
BstHHI GCGC 2 cut(s) 262, 1073
BstKTI GATC 5 cut(s) 289, 372, 395, 661, 941
BstMAI GTCTC 1 cut(s) 975
BstMBI GATC 5 cut(s) 286, 369, 392, 658, 938
BstMCI CGRYCG 1 cut(s) 1361
BstMWI GCNNNNNNNGC 4 cut(s) 37, 950, 1052, 1077
BstNI CCWGG 4 cut(s) 347, 527, 1252, 1497
BstNSI RCATGY 1 cut(s) 483
BstPI GGTNACC 1 cut(s) 1082
BstSCI CCNGG 5 cut(s) 345, 525, 1219, 1250, 1495
BstSFI CTRYAG 2 cut(s) 720, 1604
BstSLI GKGCMC 1 cut(s) 1107
BstUI CGCG 1 cut(s) 260
BstV1I GCAGC 4 cut(s) 445, 1042, 1294, 1590
BstV2I GAAGAC 3 cut(s) 126, 405, 857
BstX2I RGATCY 1 cut(s) 938
BstYI RGATCY 1 cut(s) 938
BstZ17I GTATAC 1 cut(s) 1372
BstZI CGGCCG 1 cut(s) 1358
BsuI GTATCC 1 cut(s) 1264
BsuRI GGCC 4 cut(s) 446, 609, 1356, 1360
BtgI CCRYGG 1 cut(s) 1361
BtsCI GGATG 3 cut(s) 12, 225, 442
BtsIMutI CAGTG 6 cut(s) 460, 757, 888, 897, 1342, 1429
BveI ACCTGC 1 cut(s) 1580
Cac8I GCNNGC 1 cut(s) 1358
CaiI CAGNNNCTG 2 cut(s) 401, 719
CfoI GCGC 2 cut(s) 262, 1073
Cfr10I RCCGGY 2 cut(s) 618, 1356
Cfr13I GGNCC 7 cut(s) 444, 509, 927, 1007, 1146, 1447, 1493
CseI GACGC 1 cut(s) 611
CsiI ACCWGGT 1 cut(s) 1495
Csp6I GTAC 4 cut(s) 546, 1063, 1321, 1468
CviAII CATG 2 cut(s) 480, 642
CviQI GTAC 4 cut(s) 546, 1063, 1321, 1468
DdeI CTNAG 5 cut(s) 69, 431, 452, 1141, 1351
DpnI GATC 5 cut(s) 288, 371, 394, 660, 940
DpnII GATC 5 cut(s) 286, 369, 392, 658, 938
DrdI GACNNNNNNGTC 1 cut(s) 1438
DseDI GACNNNNNNGTC 1 cut(s) 1438
EaeI YGGCCR 1 cut(s) 1358
EagI CGGCCG 1 cut(s) 1358
Eam1104I CTCTTC 2 cut(s) 385, 1257
EarI CTCTTC 2 cut(s) 385, 1257
Ecl136II GAGCTC 1 cut(s) 678
EclXI CGGCCG 1 cut(s) 1358
Eco130I CCWWGG 1 cut(s) 1450
Eco24I GRGCYC 1 cut(s) 680
Eco32I GATATC 1 cut(s) 1138
Eco47I GGWCC 6 cut(s) 509, 927, 1007, 1146, 1447, 1493
Eco52I CGGCCG 1 cut(s) 1358
Eco53kI GAGCTC 1 cut(s) 678
Eco57I CTGAAG 2 cut(s) 212, 1060
Eco88I CYCGRG 2 cut(s) 679, 697
Eco91I GGTNACC 1 cut(s) 1082
EcoICRI GAGCTC 1 cut(s) 678
EcoNI CCTNNNNNAGG 1 cut(s) 1500
EcoO109I RGGNCCY 2 cut(s) 444, 1447
EcoO65I GGTNACC 1 cut(s) 1082
EcoRII CCWGG 4 cut(s) 345, 525, 1250, 1495
EcoRV GATATC 1 cut(s) 1138
EcoT14I CCWWGG 1 cut(s) 1450
EcoT22I ATGCAT 1 cut(s) 1561
EcoT38I GRGCYC 1 cut(s) 680
ErhI CCWWGG 1 cut(s) 1450
FaeI CATG 2 cut(s) 483, 645
FalI AAGNNNNNCTT 2 cut(s) 1058, 1090
FaqI GGGAC 5 cut(s) 1159, 1236, 1268, 1460, 1519
FatI CATG 2 cut(s) 479, 641
FblI GTMKAC 1 cut(s) 1371
Fnu4HI GCNGC 5 cut(s) 459, 1056, 1173, 1308, 1604
FokI GGATG 2 cut(s) 212, 449
FriOI GRGCYC 1 cut(s) 680
FseI GGCCGGCC 1 cut(s) 1360
Fsp4HI GCNGC 5 cut(s) 459, 1056, 1173, 1308, 1604
FspBI CTAG 4 cut(s) 543, 1377, 1391, 1451
GlaI GCGC 2 cut(s) 261, 1072
GluI GCNGC 5 cut(s) 459, 1056, 1173, 1308, 1604
GsuI CTGGAG 2 cut(s) 423, 692
HaeIII GGCC 4 cut(s) 446, 609, 1356, 1360
HapII CCGG 5 cut(s) 619, 664, 1220, 1275, 1357
HgaI GACGC 1 cut(s) 611
HhaI GCGC 2 cut(s) 262, 1073
Hin1I GRCGYC 1 cut(s) 622
Hin1II CATG 2 cut(s) 483, 645
Hin6I GCGC 2 cut(s) 260, 1071
HinP1I GCGC 2 cut(s) 260, 1071
HinfI GANTC 8 cut(s) 185, 405, 497, 586, 667, 1256, 1278, 1394
HpaII CCGG 5 cut(s) 619, 664, 1220, 1275, 1357
HphI GGTGA 4 cut(s) 695, 733, 1222, 1604
Hpy166II GTNNAC 7 cut(s) 159, 1105, 1230, 1372, 1431, 1522, 1611
Hpy188I TCNGA 9 cut(s) 268, 286, 639, 654, 938, 1115, 1261, 1579, 1649
Hpy188III TCNNGA 3 cut(s) 402, 583, 671
Hpy8I GTNNAC 7 cut(s) 159, 1105, 1230, 1372, 1431, 1522, 1611
HpyAV CCTTC 5 cut(s) 157, 482, 806, 959, 1084
HpyCH4III ACNGT 8 cut(s) 715, 892, 1119, 1227, 1234, 1337, 1384, 1573
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 950, 1052, 1077
HpyF3I CTNAG 5 cut(s) 69, 431, 452, 1141, 1351
Hsp92I GRCGYC 1 cut(s) 622
Hsp92II CATG 2 cut(s) 483, 645
HspAI GCGC 2 cut(s) 260, 1071
KroI GCCGGC 1 cut(s) 1356
KroNI GCCGGC 1 cut(s) 1358
Kzo9I GATC 5 cut(s) 286, 369, 392, 658, 938
LmnI GCTCC 3 cut(s) 338, 539, 775
Lsp1109I GCAGC 4 cut(s) 445, 1042, 1294, 1590
LweI GCATC 5 cut(s) 427, 894, 1200, 1546, 1568
MabI ACCWGGT 1 cut(s) 1495
MaeI CTAG 4 cut(s) 543, 1377, 1391, 1451
MaeIII GTNAC 5 cut(s) 782, 902, 1082, 1166, 1346
MalI GATC 5 cut(s) 288, 371, 394, 660, 940
MboI GATC 5 cut(s) 286, 369, 392, 658, 938
MfeI CAATTG 1 cut(s) 1299
MflI RGATCY 1 cut(s) 938
MhlI GDGCHC 2 cut(s) 680, 1107
MluCI AATT 7 cut(s) 82, 763, 1291, 1299, 1435, 1486, 1564
MlyI GAGTC 6 cut(s) 179, 414, 506, 595, 1250, 1403
MmeI TCCRAC 3 cut(s) 339, 662, 905
Mph1103I ATGCAT 1 cut(s) 1561
MroNI GCCGGC 1 cut(s) 1356
MroXI GAANNNNTTC 1 cut(s) 969
MseI TTAA 3 cut(s) 141, 539, 762
MspA1I CMGCKG 2 cut(s) 1080, 1175
MspI CCGG 5 cut(s) 619, 664, 1220, 1275, 1357
MspR9I CCNGG 5 cut(s) 347, 527, 1221, 1252, 1497
MunI CAATTG 1 cut(s) 1299
MvaI CCWGG 4 cut(s) 347, 527, 1252, 1497
MvnI CGCG 1 cut(s) 260
MwoI GCNNNNNNNGC 4 cut(s) 37, 950, 1052, 1077
NaeI GCCGGC 1 cut(s) 1358
NciI CCSGG 1 cut(s) 1221
NdeII GATC 5 cut(s) 286, 369, 392, 658, 938
NgoMIV GCCGGC 1 cut(s) 1356
NlaIII CATG 2 cut(s) 483, 645
NlaIV GGNNCC 2 cut(s) 1147, 1448
NsiI ATGCAT 1 cut(s) 1561
NspI RCATGY 1 cut(s) 483
NspV TTCGAA 1 cut(s) 416
PaqCI CACCTGC 1 cut(s) 1580
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PdiI GCCGGC 1 cut(s) 1358
PdmI GAANNNNTTC 1 cut(s) 969
PfeI GAWTC 2 cut(s) 667, 1278
PflFI GACNNNGTC 1 cut(s) 1337
PflMI CCANNNNNTGG 1 cut(s) 1428
PfoI TCCNGGA 1 cut(s) 345
PkrI GCNGC 5 cut(s) 460, 1057, 1174, 1309, 1605
PleI GAGTC 6 cut(s) 179, 413, 505, 594, 1250, 1402
PpsI GAGTC 6 cut(s) 179, 413, 505, 594, 1250, 1402
PpuMI RGGWCCY 1 cut(s) 1447
Psp124BI GAGCTC 1 cut(s) 680
Psp5II RGGWCCY 1 cut(s) 1447
Psp6I CCWGG 4 cut(s) 345, 525, 1250, 1495
PspEI GGTNACC 1 cut(s) 1082
PspGI CCWGG 4 cut(s) 345, 525, 1250, 1495
PspN4I GGNNCC 2 cut(s) 1147, 1448
PspPI GGNCC 7 cut(s) 444, 509, 927, 1007, 1146, 1447, 1493
PspPPI RGGWCCY 1 cut(s) 1447
PstI CTGCAG 2 cut(s) 724, 1608
PstNI CAGNNNCTG 2 cut(s) 401, 719
PsuI RGATCY 1 cut(s) 938
PsyI GACNNNGTC 1 cut(s) 1337
RigI GGCCGGCC 1 cut(s) 1360
RsaI GTAC 4 cut(s) 547, 1064, 1322, 1469
RsaNI GTAC 4 cut(s) 546, 1063, 1321, 1468
SacI GAGCTC 1 cut(s) 680
SaqAI TTAA 3 cut(s) 141, 539, 762
SatI GCNGC 5 cut(s) 459, 1056, 1173, 1308, 1604
Sau3AI GATC 5 cut(s) 286, 369, 392, 658, 938
Sau96I GGNCC 7 cut(s) 444, 509, 927, 1007, 1146, 1447, 1493
SchI GAGTC 6 cut(s) 179, 414, 506, 595, 1250, 1403
ScrFI CCNGG 5 cut(s) 347, 527, 1221, 1252, 1497
SduI GDGCHC 2 cut(s) 680, 1107
SexAI ACCWGGT 1 cut(s) 1495
SfaNI GCATC 5 cut(s) 427, 894, 1200, 1546, 1568
SfcI CTRYAG 2 cut(s) 720, 1604
SfuI TTCGAA 1 cut(s) 416
SinI GGWCC 6 cut(s) 509, 927, 1007, 1146, 1447, 1493
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
SpeI ACTAGT 1 cut(s) 542
Sse9I AATT 7 cut(s) 82, 763, 1291, 1299, 1435, 1486, 1564
SsiI CCGC 2 cut(s) 1080, 1173
SspMI CTAG 4 cut(s) 543, 1377, 1391, 1451
SstI GAGCTC 1 cut(s) 680
StyD4I CCNGG 5 cut(s) 345, 525, 1219, 1250, 1495
StyI CCWWGG 1 cut(s) 1450
TaaI ACNGT 8 cut(s) 715, 892, 1119, 1227, 1234, 1337, 1384, 1573
TaqI TCGA 2 cut(s) 183, 416
TasI AATT 7 cut(s) 82, 763, 1291, 1299, 1435, 1486, 1564
TatI WGTACW 3 cut(s) 545, 1062, 1467
TauI GCSGC 1 cut(s) 1175
TfiI GAWTC 2 cut(s) 667, 1278
Tru1I TTAA 3 cut(s) 141, 539, 762
Tru9I TTAA 3 cut(s) 141, 539, 762
TscAI CASTG 6 cut(s) 460, 757, 895, 904, 1342, 1429
TseI GCWGC 4 cut(s) 458, 1055, 1307, 1603
TspDTI ATGAA 7 cut(s) 90, 530, 840, 1402, 1573, 1605, 1611
TspGWI ACGGA 1 cut(s) 1523
TspRI CASTG 6 cut(s) 460, 757, 895, 904, 1342, 1429
Tth111I GACNNNGTC 1 cut(s) 1337
Van91I CCANNNNNTGG 1 cut(s) 1428
VneI GTGCAC 1 cut(s) 1103
VpaK11BI GGWCC 6 cut(s) 509, 927, 1007, 1146, 1447, 1493
XagI CCTNNNNNAGG 1 cut(s) 1500
XapI RAATTY 1 cut(s) 1564
XceI RCATGY 1 cut(s) 483
XmaJI CCTAGG 1 cut(s) 1450
XmiI GTMKAC 1 cut(s) 1371
XmnI GAANNNNTTC 1 cut(s) 969
XspI CTAG 4 cut(s) 543, 1377, 1391, 1451
Zsp2I ATGCAT 1 cut(s) 1561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.