Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0014883

Physical Location & Seq

Genomic Coordinates Reverse (-)

7999067 .. 8001048

Gene Structure 1982 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G008090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1653 bp

ATGGCTTCCTCCATCCAAAAAGTACTTTTGCAGTTTTTGCTTCTTTGTATAATCTTCTTCTCAACAACTAAGGCTCTCTGCAATTCTTCTTCTTCTTCTTCTTCATCAACAACTAATACTCTCAATCCCCATTTGTCTTCCATTAAATCCTTCTGCAAGTCCACTCCCTACCCCGATGTCTGTTTCGACTCACTGAAGCTCTCAATCTCCATCAACATCTCCCCCAACATCCTCACTTACCTCCTCCAATCCCTCCAAATCGCGCTATCCGAAGCCACAAAGCTCTCCGATCTTTTCTACAAGGCAGGACTTTACTCCAACATTGTTGAGAAACAAAAGGGAGCAATCCAGGACTGCAAAGACCTCCACCAGATCACACTCTCTTCCATAAAACGATCAGTTTCTGGAGTCAAGTCTTCGAACAACAAGAAACTAAGGGATGCAAGAGCCTATCTCAGTGCTGCCCTCACAAACAAGAACACATGCTTGGAAGGCTTGGAGTCTGCTTCTGGTCCTATGAAGCCAACCCTGGTCAGCTCCTTAACTAGTACATACAAGTATGTTAGCAACTCTCTTTCTGCTCTCAAGAGTCAAGTGCCTAATCCAAAAGGCCGTAAAAACCGGCGTCTGTTGGGAGTTCCGACATGGATTTCTCGGAGAGATCGCCGGATTCTGGAGAGCTCGGGTGACGAATATGACCCGAGTGAAATGCTGACAGTATCTGCAGATGGTAGTGGGAACTTCACCACCATCAGTGGTGCTGTTAATTTTGCTCCAAATAATAGTTACGACAGGACTATCATCTATGTCAAAGAAGGGGTTTATGATGAAAATGTGGAAATCCCAAGCTGGAAGACAAACATTGTTCTTCTTGGAGATGGAAGTGGCATCACTGTCATCACTGGTAACAGAAGCAATGCTGATGGTTGGACCACTTTCAGATCTGCAACAGTAGCTGTATCTGGGGAAGGCTTTCTGGCAAGAGACATAAGTATTGAAAACAGAGCAGGACCAGAGAAGCACCAAGCAGTTGCATTGAGAGTAAATGCAGACCTTGCTGCATTGTACAAGTGCGCCTTCAGCGGTTACCAAGACACAATGTATGTGCACTCTTTCAGACAGTTTTATAGAGAGTGTGATATCTCAGGGACCATAGACTACATATTTGGTAACGCTGCTGTGGTAATCCAAGATTGCAACATTATATCCAAGATGCCATTGCCGGGACAGTCCACCGTTATCACAGCACAAGCCAGGGACTCCGAAGAGGAGGATACCGGAATCTCAATACAGAATTGTTCAATTGTGGCTGCTGATGACTTGTACGCCAACAAGGACAGTGTCAAAAGTTACTTAGGCCGGCCGTGGAGGGTATACTCTAGGACGGTTTTCCTAGAGTCCTTCATTGATGAGTTCATTGACCCCAGTGGGTGGACAAATTGGTCTGGGGACCTAGGCTTGGACACTTTGTACTATGGAGAGTTTGAAAATTATGGACCTGGTTCAGGGACGGAGAGTAGAGTAACCTGGACAGGGTATCATATTATGGATTACTATGATGCATCAAATTTCACAGTATCAGAGTTCATTGCAGGTGATGAATGGCTGCAGTCCACTTCATTTCCTTATGATGATGGGATTGATGGCATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0043207 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

550

Amino Acids

60.46

Weight (kDa)

5.3

Isoelectric Point (pI)

43.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	46 - 193	2.3e-25	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	239 - 533	3.8e-136	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014883)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26440
fragaria_vesca	FvH4_1g09580
malus_domestica	MD02G1104400.v1.1 MD02G1104500.v1.1 MD15G1224800.v1.1
prunus_persica	Prupe.7G190400_v2.0.a1
pyrus_communis	pycom02g08230
rosa_chinensis	RchiOBHm_Chr2g0096441
rosa_laevigata	RLG00000016640
rosa_multiflora	Rmu_sc0004096.1_g000025
rosa_roxburghii	Rroxscaffold_2G00145650
rosa_samantha	Rh2AG104700 Rh2BG106900 Rh2CG108700 Rh2DG107800
rosa_wichuraiana	Rw2G008090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1583
AasI	GACNNNNNNGTC	1 cut(s)	1441
Acc36I	ACCTGC	1 cut(s)	1583
AccB7I	CCANNNNNTGG	1 cut(s)	1431
AccI	GTMKAC	1 cut(s)	1374
AccII	CGCG	1 cut(s)	263
AciI	CCGC	1 cut(s)	1083
AcoI	YGGCCR	1 cut(s)	1361
AcsI	RAATTY	1 cut(s)	1567
AcuI	CTGAAG	2 cut(s)	215, 1063
AcyI	GRCGYC	1 cut(s)	625
AfaI	GTAC	5 cut(s)	24, 550, 1067, 1325, 1472
AfiI	CCNNNNNNNGG	6 cut(s)	673, 1223, 1431, 1459, 1505, 1533
AgsI	TTSAA	3 cut(s)	998, 1302, 1487
AhlI	ACTAGT	1 cut(s)	545
AjnI	CCWGG	5 cut(s)	348, 528, 1253, 1498, 1526
AjuI	GAANNNNNNNTTGG	2 cut(s)	470, 502
AluBI	AGCT	6 cut(s)	199, 283, 537, 681, 849, 956
AluI	AGCT	6 cut(s)	199, 283, 537, 681, 849, 956
Alw21I	GWGCWC	2 cut(s)	683, 1110
Alw26I	GTCTC	1 cut(s)	978
Alw44I	GTGCAC	1 cut(s)	1106
AlwNI	CAGNNNCTG	3 cut(s)	404, 722, 956
Ama87I	CYCGRG	2 cut(s)	682, 700
AoxI	GGCC	3 cut(s)	610, 1357, 1361
ApaLI	GTGCAC	1 cut(s)	1106
ApeKI	GCWGC	5 cut(s)	461, 1058, 1175, 1310, 1606
ApoI	RAATTY	1 cut(s)	1567
ArsI	GACNNNNNNTTYG	2 cut(s)	1148, 1180
Asp700I	GAANNNNTTC	1 cut(s)	972
AspA2I	CCTAGG	1 cut(s)	1453
AspLEI	GCGC	2 cut(s)	265, 1076
AspS9I	GGNCC	6 cut(s)	512, 930, 1010, 1149, 1450, 1496
AsuC2I	CCSGG	1 cut(s)	1224
AsuHPI	GGTGA	3 cut(s)	698, 736, 1607
AsuII	TTCGAA	1 cut(s)	419
AvaI	CYCGRG	2 cut(s)	682, 700
AvaII	GGWCC	6 cut(s)	512, 930, 1010, 1149, 1450, 1496
AvrII	CCTAGG	1 cut(s)	1453
BaeGI	GKGCMC	1 cut(s)	1110
BanII	GRGCYC	1 cut(s)	683
BbsI	GAAGAC	3 cut(s)	129, 408, 860
Bbv12I	GWGCWC	2 cut(s)	683, 1110
BbvI	GCAGC	5 cut(s)	448, 1045, 1162, 1297, 1593
BccI	CCATC	8 cut(s)	20, 218, 722, 758, 872, 917, 1628, 1637
BceAI	ACGGC	2 cut(s)	597, 1348
BcgI	CGANNNNNNTGC	2 cut(s)	691, 725
BciT130I	CCWGG	5 cut(s)	350, 530, 1255, 1500, 1528
BciVI	GTATCC	1 cut(s)	1267
BcnI	CCSGG	1 cut(s)	1224
BcoDI	GTCTC	1 cut(s)	978
BcuI	ACTAGT	1 cut(s)	545
BfaI	CTAG	4 cut(s)	546, 1380, 1394, 1454
BfmI	CTRYAG	2 cut(s)	723, 1607
BfuAI	ACCTGC	1 cut(s)	1583
BfuI	GTATCC	1 cut(s)	1267
BglII	AGATCT	1 cut(s)	941
BisI	GCNGC	5 cut(s)	462, 1059, 1176, 1311, 1607
BlnI	CCTAGG	1 cut(s)	1453
BlsI	GCNGC	5 cut(s)	463, 1060, 1177, 1312, 1608
BmcAI	AGTACT	1 cut(s)	24
Bme1390I	CCNGG	6 cut(s)	350, 530, 1224, 1255, 1500, 1528
Bme18I	GGWCC	6 cut(s)	512, 930, 1010, 1149, 1450, 1496
BmeT110I	CYCGRG	2 cut(s)	682, 700
BmgT120I	GGNCC	6 cut(s)	512, 930, 1010, 1149, 1450, 1496
BmiI	GGNNCC	2 cut(s)	1150, 1451
BmrFI	CCNGG	6 cut(s)	350, 530, 1224, 1255, 1500, 1528
BmrI	ACTGGG	1 cut(s)	1419
BmsI	GCATC	5 cut(s)	430, 897, 1203, 1549, 1571
BmuI	ACTGGG	1 cut(s)	1419
BpiI	GAAGAC	3 cut(s)	129, 408, 860
BplI	GAGNNNNNCTC	2 cut(s)	438, 470
BpmI	CTGGAG	2 cut(s)	426, 695
Bpu14I	TTCGAA	1 cut(s)	419
BpuEI	CTTGAG	1 cut(s)	569
BpuMI	CCSGG	1 cut(s)	1224
BsaHI	GRCGYC	1 cut(s)	625
BsaJI	CCNNGG	4 cut(s)	528, 1254, 1364, 1453
BsaWI	WCCGGW	1 cut(s)	1277
Bsc4I	CCNNNNNNNGG	6 cut(s)	673, 1223, 1431, 1459, 1505, 1533
Bse118I	RCCGGY	2 cut(s)	621, 1359
Bse1I	ACTGG	2 cut(s)	907, 1425
Bse3DI	GCAATG	3 cut(s)	922, 1217, 1587
BseBI	CCWGG	5 cut(s)	350, 530, 1255, 1500, 1528
BseDI	CCNNGG	4 cut(s)	528, 1254, 1364, 1453
BseGI	GGATG	3 cut(s)	12, 228, 445
BseLI	CCNNNNNNNGG	6 cut(s)	673, 1223, 1431, 1459, 1505, 1533
BseMI	GCAATG	3 cut(s)	922, 1217, 1587
BseMII	CTCAG	2 cut(s)	469, 1158
BseNI	ACTGG	2 cut(s)	907, 1425
BseRI	GAGGAG	2 cut(s)	233, 1283
BseSI	GKGCMC	1 cut(s)	1110
BseX3I	CGGCCG	1 cut(s)	1361
BseXI	GCAGC	5 cut(s)	448, 1045, 1162, 1297, 1593
Bsh1236I	CGCG	1 cut(s)	263
Bsh1285I	CGRYCG	1 cut(s)	1364
BshFI	GGCC	3 cut(s)	612, 1359, 1363
BsiEI	CGRYCG	1 cut(s)	1364
BsiHKAI	GWGCWC	2 cut(s)	683, 1110
BsiHKCI	CYCGRG	2 cut(s)	682, 700
BsiSI	CCGG	5 cut(s)	622, 667, 1223, 1278, 1360
BslFI	GGGAC	5 cut(s)	1162, 1239, 1271, 1463, 1522
BslI	CCNNNNNNNGG	6 cut(s)	673, 1223, 1431, 1459, 1505, 1533
BsmAI	GTCTC	1 cut(s)	978
BsmFI	GGGAC	5 cut(s)	1162, 1239, 1271, 1463, 1522
BsnI	GGCC	3 cut(s)	612, 1359, 1363
BsoBI	CYCGRG	2 cut(s)	682, 700
Bsp119I	TTCGAA	1 cut(s)	419
Bsp1286I	GDGCHC	2 cut(s)	683, 1110
Bsp1407I	TGTACA	1 cut(s)	1065
Bsp143I	GATC	5 cut(s)	289, 372, 395, 661, 941
BspACI	CCGC	1 cut(s)	1083
BspANI	GGCC	3 cut(s)	612, 1359, 1363
BspCNI	CTCAG	2 cut(s)	468, 1157
BspFNI	CGCG	1 cut(s)	263
BspLI	GGNNCC	2 cut(s)	1150, 1451
BspMAI	CTGCAG	2 cut(s)	727, 1611
BspMI	ACCTGC	1 cut(s)	1583
BspT104I	TTCGAA	1 cut(s)	419
BsrDI	GCAATG	3 cut(s)	922, 1217, 1587
BsrFI	RCCGGY	2 cut(s)	621, 1359
BsrGI	TGTACA	1 cut(s)	1065
BsrI	ACTGG	2 cut(s)	907, 1425
BssAI	RCCGGY	2 cut(s)	621, 1359
BssECI	CCNNGG	4 cut(s)	528, 1254, 1364, 1453
BssMI	GATC	5 cut(s)	289, 372, 395, 661, 941
BssNAI	GTATAC	1 cut(s)	1375
BssNI	GRCGYC	1 cut(s)	625
BssT1I	CCWWGG	1 cut(s)	1453
Bst1107I	GTATAC	1 cut(s)	1375
Bst2UI	CCWGG	5 cut(s)	350, 530, 1255, 1500, 1528
Bst4CI	ACNGT	9 cut(s)	718, 895, 952, 1122, 1230, 1237, 1340, 1387, 1576
Bst6I	CTCTTC	2 cut(s)	388, 1260
BstACI	GRCGYC	1 cut(s)	625
BstAPI	GCANNNNNTGC	2 cut(s)	37, 1055
BstAUI	TGTACA	1 cut(s)	1065
BstBI	TTCGAA	1 cut(s)	419
BstC8I	GCNNGC	1 cut(s)	1361
BstDEI	CTNAG	5 cut(s)	69, 434, 455, 1144, 1354
BstDSI	CCRYGG	1 cut(s)	1364
BstEII	GGTNACC	1 cut(s)	1085
BstENI	CCTNNNNNAGG	1 cut(s)	1503
BstF5I	GGATG	3 cut(s)	12, 228, 445
BstFNI	CGCG	1 cut(s)	263
BstHHI	GCGC	2 cut(s)	265, 1076
BstKTI	GATC	5 cut(s)	292, 375, 398, 664, 944
BstMAI	GTCTC	1 cut(s)	978
BstMBI	GATC	5 cut(s)	289, 372, 395, 661, 941
BstMCI	CGRYCG	1 cut(s)	1364
BstMWI	GCNNNNNNNGC	5 cut(s)	37, 492, 953, 1055, 1080
BstNI	CCWGG	5 cut(s)	350, 530, 1255, 1500, 1528
BstNSI	RCATGY	1 cut(s)	486
BstPI	GGTNACC	1 cut(s)	1085
BstSCI	CCNGG	6 cut(s)	348, 528, 1222, 1253, 1498, 1526
BstSFI	CTRYAG	2 cut(s)	723, 1607
BstSLI	GKGCMC	1 cut(s)	1110
BstUI	CGCG	1 cut(s)	263
BstV1I	GCAGC	5 cut(s)	448, 1045, 1162, 1297, 1593
BstV2I	GAAGAC	3 cut(s)	129, 408, 860
BstX2I	RGATCY	1 cut(s)	941
BstYI	RGATCY	1 cut(s)	941
BstZ17I	GTATAC	1 cut(s)	1375
BstZI	CGGCCG	1 cut(s)	1361
BsuI	GTATCC	1 cut(s)	1267
BsuRI	GGCC	3 cut(s)	612, 1359, 1363
BtgI	CCRYGG	1 cut(s)	1364
BtsCI	GGATG	3 cut(s)	12, 228, 445
BtsIMutI	CAGTG	7 cut(s)	191, 463, 760, 891, 900, 1345, 1432
BveI	ACCTGC	1 cut(s)	1583
Cac8I	GCNNGC	1 cut(s)	1361
CaiI	CAGNNNCTG	3 cut(s)	404, 722, 956
CfoI	GCGC	2 cut(s)	265, 1076
Cfr10I	RCCGGY	2 cut(s)	621, 1359
Cfr13I	GGNCC	6 cut(s)	512, 930, 1010, 1149, 1450, 1496
CseI	GACGC	1 cut(s)	614
CsiI	ACCWGGT	1 cut(s)	1498
Csp6I	GTAC	5 cut(s)	23, 549, 1066, 1324, 1471
CviAII	CATG	2 cut(s)	483, 645
CviQI	GTAC	5 cut(s)	23, 549, 1066, 1324, 1471
DdeI	CTNAG	5 cut(s)	69, 434, 455, 1144, 1354
DpnI	GATC	5 cut(s)	291, 374, 397, 663, 943
DpnII	GATC	5 cut(s)	289, 372, 395, 661, 941
DrdI	GACNNNNNNGTC	1 cut(s)	1441
DseDI	GACNNNNNNGTC	1 cut(s)	1441
EaeI	YGGCCR	1 cut(s)	1361
EagI	CGGCCG	1 cut(s)	1361
Eam1104I	CTCTTC	2 cut(s)	388, 1260
EarI	CTCTTC	2 cut(s)	388, 1260
Ecl136II	GAGCTC	1 cut(s)	681
EclXI	CGGCCG	1 cut(s)	1361
Eco130I	CCWWGG	1 cut(s)	1453
Eco24I	GRGCYC	1 cut(s)	683
Eco32I	GATATC	1 cut(s)	1141
Eco47I	GGWCC	6 cut(s)	512, 930, 1010, 1149, 1450, 1496
Eco52I	CGGCCG	1 cut(s)	1361
Eco53kI	GAGCTC	1 cut(s)	681
Eco57I	CTGAAG	2 cut(s)	215, 1063
Eco88I	CYCGRG	2 cut(s)	682, 700
Eco91I	GGTNACC	1 cut(s)	1085
EcoICRI	GAGCTC	1 cut(s)	681
EcoNI	CCTNNNNNAGG	1 cut(s)	1503
EcoO109I	RGGNCCY	1 cut(s)	1450
EcoO65I	GGTNACC	1 cut(s)	1085
EcoRII	CCWGG	5 cut(s)	348, 528, 1253, 1498, 1526
EcoRV	GATATC	1 cut(s)	1141
EcoT14I	CCWWGG	1 cut(s)	1453
EcoT22I	ATGCAT	1 cut(s)	1564
EcoT38I	GRGCYC	1 cut(s)	683
ErhI	CCWWGG	1 cut(s)	1453
FaeI	CATG	2 cut(s)	486, 648
FalI	AAGNNNNNCTT	2 cut(s)	1061, 1093
FaqI	GGGAC	5 cut(s)	1162, 1239, 1271, 1463, 1522
FatI	CATG	2 cut(s)	482, 644
FblI	GTMKAC	1 cut(s)	1374
Fnu4HI	GCNGC	5 cut(s)	462, 1059, 1176, 1311, 1607
FokI	GGATG	2 cut(s)	215, 452
FriOI	GRGCYC	1 cut(s)	683
FseI	GGCCGGCC	1 cut(s)	1363
Fsp4HI	GCNGC	5 cut(s)	462, 1059, 1176, 1311, 1607
FspBI	CTAG	4 cut(s)	546, 1380, 1394, 1454
GlaI	GCGC	2 cut(s)	264, 1075
GluI	GCNGC	5 cut(s)	462, 1059, 1176, 1311, 1607
GsuI	CTGGAG	2 cut(s)	426, 695
HaeIII	GGCC	3 cut(s)	612, 1359, 1363
HapII	CCGG	5 cut(s)	622, 667, 1223, 1278, 1360
HgaI	GACGC	1 cut(s)	614
HhaI	GCGC	2 cut(s)	265, 1076
Hin1I	GRCGYC	1 cut(s)	625
Hin1II	CATG	2 cut(s)	486, 648
Hin6I	GCGC	2 cut(s)	263, 1074
HinP1I	GCGC	2 cut(s)	263, 1074
HinfI	GANTC	8 cut(s)	188, 408, 500, 589, 670, 1259, 1281, 1397
HpaII	CCGG	5 cut(s)	622, 667, 1223, 1278, 1360
HphI	GGTGA	3 cut(s)	698, 736, 1607
Hpy166II	GTNNAC	6 cut(s)	162, 1108, 1233, 1375, 1434, 1614
Hpy188I	TCNGA	9 cut(s)	271, 289, 642, 657, 941, 1118, 1264, 1582, 1652
Hpy188III	TCNNGA	3 cut(s)	405, 586, 674
Hpy8I	GTNNAC	6 cut(s)	162, 1108, 1233, 1375, 1434, 1614
HpyAV	CCTTC	6 cut(s)	160, 485, 809, 962, 1087, 1411
HpyCH4III	ACNGT	9 cut(s)	718, 895, 952, 1122, 1230, 1237, 1340, 1387, 1576
HpyF10VI	GCNNNNNNNGC	5 cut(s)	37, 492, 953, 1055, 1080
HpyF3I	CTNAG	5 cut(s)	69, 434, 455, 1144, 1354
Hsp92I	GRCGYC	1 cut(s)	625
Hsp92II	CATG	2 cut(s)	486, 648
HspAI	GCGC	2 cut(s)	263, 1074
KroI	GCCGGC	1 cut(s)	1359
KroNI	GCCGGC	1 cut(s)	1361
Kzo9I	GATC	5 cut(s)	289, 372, 395, 661, 941
LmnI	GCTCC	3 cut(s)	341, 542, 778
Lsp1109I	GCAGC	5 cut(s)	448, 1045, 1162, 1297, 1593
LweI	GCATC	5 cut(s)	430, 897, 1203, 1549, 1571
MabI	ACCWGGT	1 cut(s)	1498
MaeI	CTAG	4 cut(s)	546, 1380, 1394, 1454
MaeIII	GTNAC	7 cut(s)	686, 785, 905, 1085, 1169, 1349, 1522
MalI	GATC	5 cut(s)	291, 374, 397, 663, 943
MboI	GATC	5 cut(s)	289, 372, 395, 661, 941
MfeI	CAATTG	1 cut(s)	1302
MflI	RGATCY	1 cut(s)	941
MhlI	GDGCHC	2 cut(s)	683, 1110
MluCI	AATT	7 cut(s)	82, 766, 1294, 1302, 1438, 1489, 1567
MlyI	GAGTC	6 cut(s)	182, 417, 509, 598, 1253, 1406
MmeI	TCCRAC	3 cut(s)	342, 665, 908
Mph1103I	ATGCAT	1 cut(s)	1564
MroNI	GCCGGC	1 cut(s)	1359
MroXI	GAANNNNTTC	1 cut(s)	972
MseI	TTAA	3 cut(s)	144, 542, 765
MspA1I	CMGCKG	1 cut(s)	1083
MspI	CCGG	5 cut(s)	622, 667, 1223, 1278, 1360
MspR9I	CCNGG	6 cut(s)	350, 530, 1224, 1255, 1500, 1528
MunI	CAATTG	1 cut(s)	1302
MvaI	CCWGG	5 cut(s)	350, 530, 1255, 1500, 1528
MvnI	CGCG	1 cut(s)	263
MwoI	GCNNNNNNNGC	5 cut(s)	37, 492, 953, 1055, 1080
NaeI	GCCGGC	1 cut(s)	1361
NciI	CCSGG	1 cut(s)	1224
NdeII	GATC	5 cut(s)	289, 372, 395, 661, 941
NgoMIV	GCCGGC	1 cut(s)	1359
NlaIII	CATG	2 cut(s)	486, 648
NlaIV	GGNNCC	2 cut(s)	1150, 1451
NmuCI	GTSAC	1 cut(s)	686
NsiI	ATGCAT	1 cut(s)	1564
NspI	RCATGY	1 cut(s)	486
NspV	TTCGAA	1 cut(s)	419
PaqCI	CACCTGC	1 cut(s)	1583
PcsI	WCGNNNNNNNCGW	1 cut(s)	267
PdiI	GCCGGC	1 cut(s)	1361
PdmI	GAANNNNTTC	1 cut(s)	972
PfeI	GAWTC	2 cut(s)	670, 1281
PflFI	GACNNNGTC	1 cut(s)	1340
PflMI	CCANNNNNTGG	1 cut(s)	1431
PfoI	TCCNGGA	1 cut(s)	348
PkrI	GCNGC	5 cut(s)	463, 1060, 1177, 1312, 1608
PleI	GAGTC	6 cut(s)	182, 416, 508, 597, 1253, 1405
PpsI	GAGTC	6 cut(s)	182, 416, 508, 597, 1253, 1405
PpuMI	RGGWCCY	1 cut(s)	1450
Psp124BI	GAGCTC	1 cut(s)	683
Psp5II	RGGWCCY	1 cut(s)	1450
Psp6I	CCWGG	5 cut(s)	348, 528, 1253, 1498, 1526
PspEI	GGTNACC	1 cut(s)	1085
PspGI	CCWGG	5 cut(s)	348, 528, 1253, 1498, 1526
PspN4I	GGNNCC	2 cut(s)	1150, 1451
PspPI	GGNCC	6 cut(s)	512, 930, 1010, 1149, 1450, 1496
PspPPI	RGGWCCY	1 cut(s)	1450
PstI	CTGCAG	2 cut(s)	727, 1611
PstNI	CAGNNNCTG	3 cut(s)	404, 722, 956
PsuI	RGATCY	1 cut(s)	941
PsyI	GACNNNGTC	1 cut(s)	1340
RigI	GGCCGGCC	1 cut(s)	1363
RsaI	GTAC	5 cut(s)	24, 550, 1067, 1325, 1472
RsaNI	GTAC	5 cut(s)	23, 549, 1066, 1324, 1471
SacI	GAGCTC	1 cut(s)	683
SaqAI	TTAA	3 cut(s)	144, 542, 765
SatI	GCNGC	5 cut(s)	462, 1059, 1176, 1311, 1607
Sau3AI	GATC	5 cut(s)	289, 372, 395, 661, 941
Sau96I	GGNCC	6 cut(s)	512, 930, 1010, 1149, 1450, 1496
ScaI	AGTACT	1 cut(s)	24
SchI	GAGTC	6 cut(s)	182, 417, 509, 598, 1253, 1406
ScrFI	CCNGG	6 cut(s)	350, 530, 1224, 1255, 1500, 1528
SduI	GDGCHC	2 cut(s)	683, 1110
SexAI	ACCWGGT	1 cut(s)	1498
SfaNI	GCATC	5 cut(s)	430, 897, 1203, 1549, 1571
SfcI	CTRYAG	2 cut(s)	723, 1607
SfuI	TTCGAA	1 cut(s)	419
SinI	GGWCC	6 cut(s)	512, 930, 1010, 1149, 1450, 1496
SmlI	CTYRAG	1 cut(s)	584
SmoI	CTYRAG	1 cut(s)	584
SpeI	ACTAGT	1 cut(s)	545
Sse9I	AATT	7 cut(s)	82, 766, 1294, 1302, 1438, 1489, 1567
SsiI	CCGC	1 cut(s)	1083
SspMI	CTAG	4 cut(s)	546, 1380, 1394, 1454
SstI	GAGCTC	1 cut(s)	683
StyD4I	CCNGG	6 cut(s)	348, 528, 1222, 1253, 1498, 1526
StyI	CCWWGG	1 cut(s)	1453
TaaI	ACNGT	9 cut(s)	718, 895, 952, 1122, 1230, 1237, 1340, 1387, 1576
TaqI	TCGA	2 cut(s)	186, 419
TasI	AATT	7 cut(s)	82, 766, 1294, 1302, 1438, 1489, 1567
TatI	WGTACW	4 cut(s)	22, 548, 1065, 1470
TfiI	GAWTC	2 cut(s)	670, 1281
Tru1I	TTAA	3 cut(s)	144, 542, 765
Tru9I	TTAA	3 cut(s)	144, 542, 765
TscAI	CASTG	7 cut(s)	198, 463, 760, 898, 907, 1345, 1432
TseFI	GTSAC	1 cut(s)	686
TseI	GCWGC	5 cut(s)	461, 1058, 1175, 1310, 1606
Tsp45I	GTSAC	1 cut(s)	686
TspDTI	ATGAA	8 cut(s)	93, 533, 843, 1393, 1405, 1576, 1608, 1614
TspGWI	ACGGA	1 cut(s)	1526
TspRI	CASTG	7 cut(s)	198, 463, 760, 898, 907, 1345, 1432
Tth111I	GACNNNGTC	1 cut(s)	1340
Van91I	CCANNNNNTGG	1 cut(s)	1431
VneI	GTGCAC	1 cut(s)	1106
VpaK11BI	GGWCC	6 cut(s)	512, 930, 1010, 1149, 1450, 1496
XagI	CCTNNNNNAGG	1 cut(s)	1503
XapI	RAATTY	1 cut(s)	1567
XceI	RCATGY	1 cut(s)	486
XmaJI	CCTAGG	1 cut(s)	1453
XmiI	GTMKAC	1 cut(s)	1374
XmnI	GAANNNNTTC	1 cut(s)	972
XspI	CTAG	4 cut(s)	546, 1380, 1394, 1454
ZrmI	AGTACT	1 cut(s)	24
Zsp2I	ATGCAT	1 cut(s)	1564

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.