Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0014883

Physical Location & Seq

Genomic Coordinates Reverse (-)

8414309 .. 8416425

Gene Structure 2117 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1104500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1623 bp

ATGGCTTCCTCCACTGCAAAACTGCTGCAGCTCCTCTGCATAATCTTATTTCTCCACATTTCTTCCTCTTCTTCTTGGTCTTCATCTTCGAACAGGACTACTTTTAATCCCCATTTGTCGTCCATAAGATCATTCTGCAAGTCCACACCCTACCGAAACATCTGCTTTGACTCACTAAAACTCTCAGTCTCCATAAACATCACTCCCAACATCCTCTCCTACCTCCTCCAATCCCTCAACGTCGCCATCTCCGAAGCCGCAAAGCTCACCGATCTTTTCTACAAGGCTGGACAGTACTCGAACATCGTCGAGAAGCAAAAGGGAGCTGTCCAGGACTGCCAGGAGCTCCACCAAATCACTCTCTCCTCCTTACAAAGATCCGTGTCTCGAATCCAGGCACGAAATGCCAAGAAACTTAACGATGCTAGGGCTTACCTCAGCGCTTCTCTCACAAACAAGAACACTTGCATGGAAGGATTGGATTCTGCTTCTGGCCCTATGAAGTCAACCCTAGTCAACTCCTTGACTAGTACATACAAATATGTAAGCAATTCTCTCTCAGTTATCTCAAAGCAAGGGGCGAATAAAGGGCGCCCAAACCGCCGTCTTATGAGAGTTCCGAGGTGGTTATCGAGGAGAGACCGCCGGATATTGGAGAGCTCCGGCGATGAATATGACCCCAGTGAAGTCCTAACGGTTGCCGCAGATGGATCGGGGAACTTCACCACAATTAGTGATGCTATTATGTTTTGTCCAAATAATAGTTATGATAGGACGATTATATATGTCAAAGAAGGGGTTTATGAAGAAAATGTGGAGATTCCGAGCTACAAGACCAACATTGCTCTTTTTGGAGATGGAAGTGACATCACTTTCATCACTGGTAACCGCAGTCGTGGTGATGGATGGACTACATTCAGATCTGCAACCGTAGCTGTGTCCGGAGAGGGCTTTCTAGCAAGAGACATAACAATTCAGAACACGGCAGGGCCAGAAAAGCTCCAAGCAGTTGCTCTGAGAATGAATGCAGACTTGGTTGCAATTTACAAATGCACTATCAATGGCTACCAAGACACGTTATACGTGCACTCTTTCAGACAATTCTACAGAGAGTGCGATATATTCGGAACCATAGACTACATATGTGGTAACGCTGCAGTTGTATTTCAAGCATGCAACATCGTGTCTAAGATGCCAATGCCGGGACAATTCACAGTCGTCACGGCGCAGTCCAGGGACACATTAGATCAGAACACAGGAATCTCCATGCAGAACTGCTCCATTGTAGCCAGTGATGAGTTGTACTCCAATTCCAGCATTGTCAAAAGCTACTTAGGCCGGCCGTGGAGGAATTACTCTACGACTGTTGTGCTGAAGTCCTACATTGACGACTTTATTGACCCGACTGGGTGGACTAATTGGGCCGGGGACCAAGGATTGGACACTTTGTACTATGGAGAGTATGAGAATTCTGGTCCTGGCGCTGGGACGGATAATAGAGTGAACTGGACAGGGTATCATGTTATGGACTACTATGATGCATCCAATTTCACGGTGCAAGAGTTTATTGCTGGTGATGAATGGCTGCAGTCCACTTCATTTCCTTATGATGATGGCATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0043207 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

541

Amino Acids

59.98

Weight (kDa)

6.05

Isoelectric Point (pI)

43.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	41 - 187	6e-23	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	232 - 526	4.1e-135	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014883)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26440
fragaria_vesca	FvH4_1g09580
malus_domestica	MD02G1104400.v1.1 MD02G1104500.v1.1 MD15G1224800.v1.1
prunus_persica	Prupe.7G190400_v2.0.a1
pyrus_communis	pycom02g08230
rosa_chinensis	RchiOBHm_Chr2g0096441
rosa_laevigata	RLG00000016640
rosa_multiflora	Rmu_sc0004096.1_g000025
rosa_roxburghii	Rroxscaffold_2G00145650
rosa_samantha	Rh2AG104700 Rh2BG106900 Rh2CG108700 Rh2DG107800
rosa_wichuraiana	Rw2G008090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	591
AccB7I	CCANNNNNTGG	1 cut(s)	1438
AccIII	TCCGGA	1 cut(s)	941
AciI	CCGC	5 cut(s)	258, 601, 643, 702, 889
AclWI	GGATC	2 cut(s)	372, 718
AcoI	YGGCCR	1 cut(s)	1340
AcsI	RAATTY	1 cut(s)	1468
AcuI	CTGAAG	1 cut(s)	1394
AcyI	GRCGYC	1 cut(s)	592
AfaI	GTAC	4 cut(s)	296, 532, 1304, 1451
AfeI	AGCGCT	1 cut(s)	442
AfiI	CCNNNNNNNGG	4 cut(s)	652, 1202, 1438, 1484
AflIII	ACRYGT	1 cut(s)	1074
AgsI	TTSAA	1 cut(s)	1169
AhlI	ACTAGT	1 cut(s)	527
AjnI	CCWGG	5 cut(s)	330, 339, 393, 1232, 1477
AjuI	GAANNNNNNNTTGG	2 cut(s)	1016, 1048
AluBI	AGCT	9 cut(s)	31, 265, 326, 346, 660, 828, 935, 1000, 1329
AluI	AGCT	9 cut(s)	31, 265, 326, 346, 660, 828, 935, 1000, 1329
Alw21I	GWGCWC	3 cut(s)	348, 662, 1089
Alw26I	GTCTC	4 cut(s)	193, 390, 633, 957
Alw44I	GTGCAC	1 cut(s)	1085
AlwI	GGATC	2 cut(s)	372, 718
Aor13HI	TCCGGA	1 cut(s)	941
Aor51HI	AGCGCT	1 cut(s)	442
AoxI	GGCC	5 cut(s)	493, 989, 1336, 1340, 1422
ApaLI	GTGCAC	1 cut(s)	1085
ApeKI	GCWGC	4 cut(s)	25, 28, 1154, 1585
ApoI	RAATTY	1 cut(s)	1468
AspLEI	GCGC	4 cut(s)	443, 594, 1228, 1484
AspS9I	GGNCC	5 cut(s)	494, 989, 1422, 1429, 1475
AsuC2I	CCSGG	2 cut(s)	1203, 1426
AsuHPI	GGTGA	4 cut(s)	259, 715, 911, 1586
AsuII	TTCGAA	1 cut(s)	89
AvaII	GGWCC	2 cut(s)	1429, 1475
BaeGI	GKGCMC	1 cut(s)	1089
BanI	GGYRCC	1 cut(s)	591
BanII	GRGCYC	2 cut(s)	348, 662
BbsI	GAAGAC	1 cut(s)	72
Bbv12I	GWGCWC	3 cut(s)	348, 662, 1089
BbvCI	CCTCAGC	1 cut(s)	437
BbvI	GCAGC	4 cut(s)	12, 40, 1141, 1572
BccI	CCATC	6 cut(s)	254, 701, 851, 896, 900, 1607
BceAI	ACGGC	4 cut(s)	588, 999, 1239, 1327
BcgI	CGANNNNNNTGC	6 cut(s)	144, 178, 693, 727, 1351, 1385
BciT130I	CCWGG	5 cut(s)	332, 341, 395, 1234, 1479
BcnI	CCSGG	2 cut(s)	1203, 1426
BcoDI	GTCTC	4 cut(s)	193, 390, 633, 957
BcuI	ACTAGT	1 cut(s)	527
BfaI	CTAG	4 cut(s)	426, 512, 528, 956
BfmI	CTRYAG	4 cut(s)	26, 1105, 1155, 1586
BfoI	RGCGCY	3 cut(s)	444, 595, 1485
BglII	AGATCT	1 cut(s)	920
BisI	GCNGC	6 cut(s)	26, 29, 258, 702, 1155, 1586
BlsI	GCNGC	6 cut(s)	27, 30, 259, 703, 1156, 1587
BmcAI	AGTACT	1 cut(s)	296
Bme1390I	CCNGG	7 cut(s)	332, 341, 395, 1203, 1234, 1426, 1479
Bme18I	GGWCC	2 cut(s)	1429, 1475
BmgT120I	GGNCC	5 cut(s)	494, 989, 1422, 1429, 1475
BmiI	GGNNCC	3 cut(s)	593, 1129, 1430
BmrFI	CCNGG	7 cut(s)	332, 341, 395, 1203, 1234, 1426, 1479
BmrI	ACTGGG	2 cut(s)	675, 1416
BmsI	GCATC	5 cut(s)	412, 727, 1182, 1528, 1550
BmuI	ACTGGG	2 cut(s)	675, 1416
BpiI	GAAGAC	1 cut(s)	72
BplI	GAGNNNNNCTC	2 cut(s)	1289, 1321
Bpu10I	CCTNAGC	1 cut(s)	437
Bpu14I	TTCGAA	1 cut(s)	89
BpuMI	CCSGG	2 cut(s)	1203, 1426
BsaAI	YACGTR	1 cut(s)	1084
BsaHI	GRCGYC	1 cut(s)	592
BsaI	GGTCTC	1 cut(s)	633
BsaJI	CCNNGG	5 cut(s)	620, 1233, 1343, 1425, 1432
BsaWI	WCCGGW	1 cut(s)	941
BsaXI	ACNNNNNCTCC	2 cut(s)	200, 230
Bsc4I	CCNNNNNNNGG	4 cut(s)	652, 1202, 1438, 1484
Bse118I	RCCGGY	1 cut(s)	1338
Bse1I	ACTGG	5 cut(s)	681, 886, 1290, 1411, 1511
Bse3DI	GCAATG	1 cut(s)	840
BseAI	TCCGGA	1 cut(s)	941
BseBI	CCWGG	5 cut(s)	332, 341, 395, 1234, 1479
BseDI	CCNNGG	5 cut(s)	620, 1233, 1343, 1425, 1432
BseGI	GGATG	3 cut(s)	210, 911, 1541
BseLI	CCNNNNNNNGG	4 cut(s)	652, 1202, 1438, 1484
BseMI	GCAATG	1 cut(s)	840
BseMII	CTCAG	4 cut(s)	198, 451, 573, 1007
BseNI	ACTGG	5 cut(s)	681, 886, 1290, 1411, 1511
BseRI	GAGGAG	4 cut(s)	23, 215, 355, 649
BseSI	GKGCMC	1 cut(s)	1089
BseX3I	CGGCCG	1 cut(s)	1340
BseXI	GCAGC	4 cut(s)	12, 40, 1141, 1572
BseYI	CCCAGC	1 cut(s)	1484
Bsh1285I	CGRYCG	1 cut(s)	1343
BshFI	GGCC	5 cut(s)	495, 991, 1338, 1342, 1424
BshNI	GGYRCC	1 cut(s)	591
BsiEI	CGRYCG	1 cut(s)	1343
BsiHKAI	GWGCWC	3 cut(s)	348, 662, 1089
BsiSI	CCGG	6 cut(s)	646, 663, 942, 1202, 1339, 1425
BslFI	GGGAC	4 cut(s)	1218, 1250, 1442, 1501
BslI	CCNNNNNNNGG	4 cut(s)	652, 1202, 1438, 1484
BsmAI	GTCTC	4 cut(s)	193, 390, 633, 957
BsmFI	GGGAC	4 cut(s)	1218, 1250, 1442, 1501
BsmI	GAATGC	1 cut(s)	1030
BsnI	GGCC	5 cut(s)	495, 991, 1338, 1342, 1424
Bso31I	GGTCTC	1 cut(s)	633
Bsp119I	TTCGAA	1 cut(s)	89
Bsp1286I	GDGCHC	3 cut(s)	348, 662, 1089
Bsp13I	TCCGGA	1 cut(s)	941
Bsp143I	GATC	6 cut(s)	128, 271, 377, 710, 920, 1246
BspACI	CCGC	5 cut(s)	258, 601, 643, 702, 889
BspANI	GGCC	5 cut(s)	495, 991, 1338, 1342, 1424
BspCNI	CTCAG	4 cut(s)	197, 450, 572, 1008
BspEI	TCCGGA	1 cut(s)	941
BspLI	GGNNCC	3 cut(s)	593, 1129, 1430
BspMAI	CTGCAG	3 cut(s)	30, 1159, 1590
BspPI	GGATC	2 cut(s)	372, 718
BspT104I	TTCGAA	1 cut(s)	89
BspT107I	GGYRCC	1 cut(s)	591
BspTNI	GGTCTC	1 cut(s)	633
BsrDI	GCAATG	1 cut(s)	840
BsrFI	RCCGGY	1 cut(s)	1338
BsrI	ACTGG	5 cut(s)	681, 886, 1290, 1411, 1511
BssAI	RCCGGY	1 cut(s)	1338
BssECI	CCNNGG	5 cut(s)	620, 1233, 1343, 1425, 1432
BssMI	GATC	6 cut(s)	128, 271, 377, 710, 920, 1246
BssNI	GRCGYC	1 cut(s)	592
BssT1I	CCWWGG	1 cut(s)	1432
Bst2UI	CCWGG	5 cut(s)	332, 341, 395, 1234, 1479
Bst4CI	ACNGT	6 cut(s)	294, 697, 931, 1216, 1366, 1555
Bst6I	CTCTTC	1 cut(s)	73
BstACI	GRCGYC	1 cut(s)	592
BstAPI	GCANNNNNTGC	1 cut(s)	404
BstBAI	YACGTR	1 cut(s)	1084
BstBI	TTCGAA	1 cut(s)	89
BstC8I	GCNNGC	2 cut(s)	1174, 1340
BstDEI	CTNAG	6 cut(s)	184, 437, 559, 1016, 1188, 1333
BstDSI	CCRYGG	1 cut(s)	1343
BstEII	GGTNACC	1 cut(s)	884
BstF5I	GGATG	3 cut(s)	210, 911, 1541
BstH2I	RGCGCY	3 cut(s)	444, 595, 1485
BstHHI	GCGC	4 cut(s)	443, 594, 1228, 1484
BstKTI	GATC	6 cut(s)	131, 274, 380, 713, 923, 1249
BstMAI	GTCTC	4 cut(s)	193, 390, 633, 957
BstMBI	GATC	6 cut(s)	128, 271, 377, 710, 920, 1246
BstMCI	CGRYCG	1 cut(s)	1343
BstMWI	GCNNNNNNNGC	5 cut(s)	404, 600, 932, 997, 1335
BstNI	CCWGG	5 cut(s)	332, 341, 395, 1234, 1479
BstNSI	RCATGY	1 cut(s)	1176
BstPI	GGTNACC	1 cut(s)	884
BstSCI	CCNGG	7 cut(s)	330, 339, 393, 1201, 1232, 1424, 1477
BstSFI	CTRYAG	4 cut(s)	26, 1105, 1155, 1586
BstSLI	GKGCMC	1 cut(s)	1089
BstV1I	GCAGC	4 cut(s)	12, 40, 1141, 1572
BstV2I	GAAGAC	1 cut(s)	72
BstX2I	RGATCY	2 cut(s)	377, 920
BstYI	RGATCY	2 cut(s)	377, 920
BstZI	CGGCCG	1 cut(s)	1340
BsuRI	GGCC	5 cut(s)	495, 991, 1338, 1342, 1424
BtgI	CCRYGG	1 cut(s)	1343
BtgZI	GCGATG	1 cut(s)	681
BtsCI	GGATG	3 cut(s)	210, 911, 1541
BtsI	GCAGTG	1 cut(s)	12
BtsIMutI	CAGTG	4 cut(s)	12, 688, 879, 1297
Cac8I	GCNNGC	2 cut(s)	1174, 1340
CfoI	GCGC	4 cut(s)	443, 594, 1228, 1484
Cfr10I	RCCGGY	1 cut(s)	1338
Cfr13I	GGNCC	5 cut(s)	494, 989, 1422, 1429, 1475
Csp6I	GTAC	4 cut(s)	295, 531, 1303, 1450
CviAII	CATG	4 cut(s)	469, 1173, 1267, 1520
CviQI	GTAC	4 cut(s)	295, 531, 1303, 1450
DdeI	CTNAG	6 cut(s)	184, 437, 559, 1016, 1188, 1333
DinI	GGCGCC	1 cut(s)	593
DpnI	GATC	6 cut(s)	130, 273, 379, 712, 922, 1248
DpnII	GATC	6 cut(s)	128, 271, 377, 710, 920, 1246
EaeI	YGGCCR	1 cut(s)	1340
EagI	CGGCCG	1 cut(s)	1340
Eam1104I	CTCTTC	1 cut(s)	73
EarI	CTCTTC	1 cut(s)	73
Ecl136II	GAGCTC	2 cut(s)	346, 660
EclXI	CGGCCG	1 cut(s)	1340
Eco130I	CCWWGG	1 cut(s)	1432
Eco24I	GRGCYC	2 cut(s)	348, 662
Eco31I	GGTCTC	1 cut(s)	633
Eco47I	GGWCC	2 cut(s)	1429, 1475
Eco47III	AGCGCT	1 cut(s)	442
Eco52I	CGGCCG	1 cut(s)	1340
Eco53kI	GAGCTC	2 cut(s)	346, 660
Eco57I	CTGAAG	1 cut(s)	1394
Eco91I	GGTNACC	1 cut(s)	884
EcoICRI	GAGCTC	2 cut(s)	346, 660
EcoO65I	GGTNACC	1 cut(s)	884
EcoRI	GAATTC	1 cut(s)	1468
EcoRII	CCWGG	5 cut(s)	330, 339, 393, 1232, 1477
EcoT14I	CCWWGG	1 cut(s)	1432
EcoT22I	ATGCAT	1 cut(s)	1543
EcoT38I	GRGCYC	2 cut(s)	348, 662
EgeI	GGCGCC	1 cut(s)	593
EheI	GGCGCC	1 cut(s)	593
ErhI	CCWWGG	1 cut(s)	1432
FaeI	CATG	4 cut(s)	472, 1176, 1270, 1523
FaqI	GGGAC	4 cut(s)	1218, 1250, 1442, 1501
FatI	CATG	4 cut(s)	468, 1172, 1266, 1519
FauNDI	CATATG	1 cut(s)	1142
Fnu4HI	GCNGC	6 cut(s)	26, 29, 258, 702, 1155, 1586
FokI	GGATG	3 cut(s)	197, 918, 1528
FriOI	GRGCYC	2 cut(s)	348, 662
FseI	GGCCGGCC	1 cut(s)	1342
Fsp4HI	GCNGC	6 cut(s)	26, 29, 258, 702, 1155, 1586
FspBI	CTAG	4 cut(s)	426, 512, 528, 956
GlaI	GCGC	4 cut(s)	442, 593, 1227, 1483
GluI	GCNGC	6 cut(s)	26, 29, 258, 702, 1155, 1586
GsaI	CCCAGC	1 cut(s)	1488
HaeII	RGCGCY	3 cut(s)	444, 595, 1485
HaeIII	GGCC	5 cut(s)	495, 991, 1338, 1342, 1424
HapII	CCGG	6 cut(s)	646, 663, 942, 1202, 1339, 1425
HhaI	GCGC	4 cut(s)	443, 594, 1228, 1484
Hin1I	GRCGYC	1 cut(s)	592
Hin1II	CATG	4 cut(s)	472, 1176, 1270, 1523
Hin6I	GCGC	4 cut(s)	441, 592, 1226, 1482
HinP1I	GCGC	4 cut(s)	441, 592, 1226, 1482
HincII	GTYRAC	2 cut(s)	507, 517
HindII	GTYRAC	2 cut(s)	507, 517
HinfI	GANTC	5 cut(s)	170, 390, 482, 820, 1260
HpaII	CCGG	6 cut(s)	646, 663, 942, 1202, 1339, 1425
HphI	GGTGA	4 cut(s)	259, 715, 911, 1586
Hpy166II	GTNNAC	7 cut(s)	144, 507, 517, 1087, 1413, 1504, 1593
Hpy188III	TCNNGA	3 cut(s)	310, 387, 942
Hpy8I	GTNNAC	7 cut(s)	144, 507, 517, 1087, 1413, 1504, 1593
Hpy99I	CGWCG	2 cut(s)	245, 311
HpyAV	CCTTC	2 cut(s)	467, 788
HpyCH4III	ACNGT	6 cut(s)	294, 697, 931, 1216, 1366, 1555
HpyCH4IV	ACGT	3 cut(s)	240, 1076, 1083
HpyF10VI	GCNNNNNNNGC	5 cut(s)	404, 600, 932, 997, 1335
HpyF3I	CTNAG	6 cut(s)	184, 437, 559, 1016, 1188, 1333
HpySE526I	ACGT	3 cut(s)	240, 1076, 1083
Hsp92I	GRCGYC	1 cut(s)	592
Hsp92II	CATG	4 cut(s)	472, 1176, 1270, 1523
HspAI	GCGC	4 cut(s)	441, 592, 1226, 1482
KasI	GGCGCC	1 cut(s)	591
Kpn2I	TCCGGA	1 cut(s)	941
KroI	GCCGGC	1 cut(s)	1338
KroNI	GCCGGC	1 cut(s)	1340
Kzo9I	GATC	6 cut(s)	128, 271, 377, 710, 920, 1246
LmnI	GCTCC	7 cut(s)	36, 323, 343, 351, 665, 1005, 1283
Lsp1109I	GCAGC	4 cut(s)	12, 40, 1141, 1572
LweI	GCATC	5 cut(s)	412, 727, 1182, 1528, 1550
MaeI	CTAG	4 cut(s)	426, 512, 528, 956
MaeII	ACGT	3 cut(s)	240, 1076, 1083
MaeIII	GTNAC	4 cut(s)	863, 884, 1148, 1219
MalI	GATC	6 cut(s)	130, 273, 379, 712, 922, 1248
MboI	GATC	6 cut(s)	128, 271, 377, 710, 920, 1246
MboII	GAAGA	6 cut(s)	54, 60, 63, 72, 78, 818
MflI	RGATCY	2 cut(s)	377, 920
MhlI	GDGCHC	3 cut(s)	348, 662, 1089
Mly113I	GGCGCC	1 cut(s)	592
MlyI	GAGTC	1 cut(s)	164
Mph1103I	ATGCAT	1 cut(s)	1543
MroI	TCCGGA	1 cut(s)	941
MroNI	GCCGGC	1 cut(s)	1338
MseI	TTAA	2 cut(s)	105, 417
MslI	CAYNNNNRTG	1 cut(s)	467
MspI	CCGG	6 cut(s)	646, 663, 942, 1202, 1339, 1425
MspR9I	CCNGG	7 cut(s)	332, 341, 395, 1203, 1234, 1426, 1479
Mva1269I	GAATGC	1 cut(s)	1030
MvaI	CCWGG	5 cut(s)	332, 341, 395, 1234, 1479
MwoI	GCNNNNNNNGC	5 cut(s)	404, 600, 932, 997, 1335
NaeI	GCCGGC	1 cut(s)	1340
NarI	GGCGCC	1 cut(s)	592
NciI	CCSGG	2 cut(s)	1203, 1426
NdeI	CATATG	1 cut(s)	1142
NdeII	GATC	6 cut(s)	128, 271, 377, 710, 920, 1246
NgoMIV	GCCGGC	1 cut(s)	1338
NlaIII	CATG	4 cut(s)	472, 1176, 1270, 1523
NlaIV	GGNNCC	3 cut(s)	593, 1129, 1430
NmuCI	GTSAC	2 cut(s)	863, 1219
NsiI	ATGCAT	1 cut(s)	1543
NspI	RCATGY	1 cut(s)	1176
NspV	TTCGAA	1 cut(s)	89
PaeI	GCATGC	1 cut(s)	1176
PcsI	WCGNNNNNNNCGW	1 cut(s)	249
PctI	GAATGC	1 cut(s)	1030
PdiI	GCCGGC	1 cut(s)	1340
PfeI	GAWTC	4 cut(s)	390, 482, 820, 1260
PflMI	CCANNNNNTGG	1 cut(s)	1438
PfoI	TCCNGGA	1 cut(s)	330
PkrI	GCNGC	6 cut(s)	27, 30, 259, 703, 1156, 1587
PleI	GAGTC	1 cut(s)	164
PluTI	GGCGCC	1 cut(s)	595
PpsI	GAGTC	1 cut(s)	164
Ppu21I	YACGTR	1 cut(s)	1084
Psp124BI	GAGCTC	2 cut(s)	348, 662
Psp6I	CCWGG	5 cut(s)	330, 339, 393, 1232, 1477
PspEI	GGTNACC	1 cut(s)	884
PspFI	CCCAGC	1 cut(s)	1484
PspGI	CCWGG	5 cut(s)	330, 339, 393, 1232, 1477
PspN4I	GGNNCC	3 cut(s)	593, 1129, 1430
PspPI	GGNCC	5 cut(s)	494, 989, 1422, 1429, 1475
PstI	CTGCAG	3 cut(s)	30, 1159, 1590
PsuI	RGATCY	2 cut(s)	377, 920
RigI	GGCCGGCC	1 cut(s)	1342
RsaI	GTAC	4 cut(s)	296, 532, 1304, 1451
RsaNI	GTAC	4 cut(s)	295, 531, 1303, 1450
RseI	CAYNNNNRTG	1 cut(s)	467
SacI	GAGCTC	2 cut(s)	348, 662
SaqAI	TTAA	2 cut(s)	105, 417
SatI	GCNGC	6 cut(s)	26, 29, 258, 702, 1155, 1586
Sau3AI	GATC	6 cut(s)	128, 271, 377, 710, 920, 1246
Sau96I	GGNCC	5 cut(s)	494, 989, 1422, 1429, 1475
ScaI	AGTACT	1 cut(s)	296
SchI	GAGTC	1 cut(s)	164
ScrFI	CCNGG	7 cut(s)	332, 341, 395, 1203, 1234, 1426, 1479
SduI	GDGCHC	3 cut(s)	348, 662, 1089
SfaNI	GCATC	5 cut(s)	412, 727, 1182, 1528, 1550
SfcI	CTRYAG	4 cut(s)	26, 1105, 1155, 1586
SfoI	GGCGCC	1 cut(s)	593
SfuI	TTCGAA	1 cut(s)	89
SinI	GGWCC	2 cut(s)	1429, 1475
SmiMI	CAYNNNNRTG	1 cut(s)	467
SpeI	ACTAGT	1 cut(s)	527
SphI	GCATGC	1 cut(s)	1176
SsiI	CCGC	5 cut(s)	258, 601, 643, 702, 889
SspDI	GGCGCC	1 cut(s)	591
SspMI	CTAG	4 cut(s)	426, 512, 528, 956
SstI	GAGCTC	2 cut(s)	348, 662
StyD4I	CCNGG	7 cut(s)	330, 339, 393, 1201, 1232, 1424, 1477
StyI	CCWWGG	1 cut(s)	1432
TaaI	ACNGT	6 cut(s)	294, 697, 931, 1216, 1366, 1555
TaiI	ACGT	3 cut(s)	243, 1079, 1086
TaqI	TCGA	5 cut(s)	89, 299, 309, 388, 632
TatI	WGTACW	4 cut(s)	294, 530, 1302, 1449
TauI	GCSGC	2 cut(s)	260, 704
TfiI	GAWTC	4 cut(s)	390, 482, 820, 1260
Tru1I	TTAA	2 cut(s)	105, 417
Tru9I	TTAA	2 cut(s)	105, 417
TscAI	CASTG	4 cut(s)	19, 688, 886, 1297
TseFI	GTSAC	2 cut(s)	863, 1219
TseI	GCWGC	4 cut(s)	25, 28, 1154, 1585
Tsp45I	GTSAC	2 cut(s)	863, 1219
TspDTI	ATGAA	8 cut(s)	72, 515, 684, 819, 865, 1037, 1587, 1593
TspGWI	ACGGA	2 cut(s)	370, 1505
TspRI	CASTG	4 cut(s)	19, 688, 886, 1297
Van91I	CCANNNNNTGG	1 cut(s)	1438
VneI	GTGCAC	1 cut(s)	1085
VpaK11BI	GGWCC	2 cut(s)	1429, 1475
XapI	RAATTY	1 cut(s)	1468
XceI	RCATGY	1 cut(s)	1176
XspI	CTAG	4 cut(s)	426, 512, 528, 956
ZrmI	AGTACT	1 cut(s)	296
Zsp2I	ATGCAT	1 cut(s)	1543

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.