pycom02g08230

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0014883
Physical Location & Seq
Reverse (-)
5749293 .. 5749892
600 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g08230.1

Sequence Viewer

Length: 600 bp
ATGCAGGCCTGGCTGCAATTTACAAATGCGCCATCGATGGCTACCAAAGACACATTATACGTGCACTCTGTCAGACAATTCTACGGAGAGTGTGATATATTCGGAACCATAGACTACATATGTGGTAACGCCGCAGTTGTATTTCAAGCATGCACCATCGTGTCCAAAATGCCAATGCCGGGACAAATCACAGTCATCACCGAGCAGTCCAGGGACACACTAGATCTGATCACAGGAATCTCCATGCAGAACTGCTCCATTGTAGCCAGTGATGAGTTGCACTCTAATTCCAGCATTGTCAAAAGCTACTTAGGCCGGCCGTGGATGATTTACTCTACGACTGTTGTCCTGAAGTCCTACATTAACGATTTTATTGACCCGACTGGGTGGACTAATTGGTCTGGGGACCTAGGGTTGGACACTTTGTACTATGGAGAGTACAAGAATTATGTTCCTGGCGCTGGGACGGATAATAGAGTGAACTGGACAAGACATCATGTTATGGACTATTATGATGCATCCAATTTCAGTGTGCAAGAGTTTATTGCTGGTGATGAATGGCTGCAGTCCACTTTATTTCCTTACGATGAAGGCATCTGA

Protein Analysis

200

Amino Acids

22.49

Weight (kDa)

4.43

Isoelectric Point (pI)

33.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 13 - 185 1.3e-69 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014883)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26440
fragaria_vesca FvH4_1g09580
malus_domestica MD02G1104400.v1.1 MD02G1104500.v1.1 MD15G1224800.v1.1
prunus_persica Prupe.7G190400_v2.0.a1
pyrus_communis pycom02g08230
rosa_chinensis RchiOBHm_Chr2g0096441
rosa_laevigata RLG00000016640
rosa_multiflora Rmu_sc0004096.1_g000025
rosa_roxburghii Rroxscaffold_2G00145650
rosa_samantha Rh2AG104700 Rh2BG106900 Rh2CG108700 Rh2DG107800
rosa_wichuraiana Rw2G008090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 397
AciI CCGC 1 cut(s) 132
AcoI YGGCCR 1 cut(s) 317
AcuI CTGAAG 1 cut(s) 371
AfaI GTAC 2 cut(s) 428, 440
AfiI CCNNNNNNNGG 3 cut(s) 179, 415, 461
AgsI TTSAA 1 cut(s) 146
AjnI CCWGG 3 cut(s) 8, 209, 454
AleI CACNNNNGTG 1 cut(s) 158
AluBI AGCT 1 cut(s) 306
AluI AGCT 1 cut(s) 306
Alw21I GWGCWC 1 cut(s) 66
Alw44I GTGCAC 1 cut(s) 62
AoxI GGCC 3 cut(s) 6, 313, 317
ApaLI GTGCAC 1 cut(s) 62
ApeKI GCWGC 2 cut(s) 13, 562
AspA2I CCTAGG 1 cut(s) 409
AspLEI GCGC 2 cut(s) 31, 461
AspS9I GGNCC 1 cut(s) 406
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 2 cut(s) 190, 563
AvaII GGWCC 1 cut(s) 406
AvrII CCTAGG 1 cut(s) 409
BaeGI GKGCMC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 1 cut(s) 549
BccI CCATC 3 cut(s) 31, 40, 164
BceAI ACGGC 1 cut(s) 304
BciT130I CCWGG 3 cut(s) 10, 211, 456
BclI TGATCA 1 cut(s) 228
BcnI CCSGG 1 cut(s) 180
BfaI CTAG 2 cut(s) 221, 410
BfmI CTRYAG 1 cut(s) 563
BfoI RGCGCY 1 cut(s) 462
BglII AGATCT 1 cut(s) 223
BisI GCNGC 3 cut(s) 14, 132, 563
BlnI CCTAGG 1 cut(s) 409
BlsI GCNGC 3 cut(s) 15, 133, 564
Bme1390I CCNGG 4 cut(s) 10, 180, 211, 456
Bme18I GGWCC 1 cut(s) 406
BmgT120I GGNCC 1 cut(s) 406
BmiI GGNNCC 2 cut(s) 106, 407
BmrFI CCNGG 4 cut(s) 10, 180, 211, 456
BmrI ACTGGG 1 cut(s) 393
BmsI GCATC 2 cut(s) 505, 527
BmuI ACTGGG 1 cut(s) 393
BoxI GACNNNNGTC 1 cut(s) 344
BplI GAGNNNNNCTC 2 cut(s) 266, 298
BpuMI CCSGG 1 cut(s) 180
Bsa29I ATCGAT 1 cut(s) 35
BsaAI YACGTR 1 cut(s) 61
BsaJI CCNNGG 3 cut(s) 210, 320, 409
Bsc4I CCNNNNNNNGG 3 cut(s) 179, 415, 461
Bse118I RCCGGY 1 cut(s) 315
Bse1I ACTGG 3 cut(s) 267, 388, 488
BseBI CCWGG 3 cut(s) 10, 211, 456
BseCI ATCGAT 1 cut(s) 35
BseDI CCNNGG 3 cut(s) 210, 320, 409
BseGI GGATG 2 cut(s) 330, 518
BseLI CCNNNNNNNGG 3 cut(s) 179, 415, 461
BseNI ACTGG 3 cut(s) 267, 388, 488
BseSI GKGCMC 1 cut(s) 66
BseX3I CGGCCG 1 cut(s) 317
BseXI GCAGC 1 cut(s) 549
BseYI CCCAGC 1 cut(s) 461
Bsh1285I CGRYCG 1 cut(s) 320
BshFI GGCC 3 cut(s) 8, 315, 319
BshVI ATCGAT 1 cut(s) 35
BsiEI CGRYCG 1 cut(s) 320
BsiHKAI GWGCWC 1 cut(s) 66
BsiSI CCGG 2 cut(s) 179, 316
BslFI GGGAC 4 cut(s) 195, 227, 419, 478
BslI CCNNNNNNNGG 3 cut(s) 179, 415, 461
BsmFI GGGAC 4 cut(s) 195, 227, 419, 478
BsnI GGCC 3 cut(s) 8, 315, 319
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 2 cut(s) 223, 228
BspACI CCGC 1 cut(s) 132
BspANI GGCC 3 cut(s) 8, 315, 319
BspDI ATCGAT 1 cut(s) 35
BspLI GGNNCC 2 cut(s) 106, 407
BspMAI CTGCAG 1 cut(s) 567
BsrFI RCCGGY 1 cut(s) 315
BsrI ACTGG 3 cut(s) 267, 388, 488
BssAI RCCGGY 1 cut(s) 315
BssECI CCNNGG 3 cut(s) 210, 320, 409
BssMI GATC 2 cut(s) 223, 228
BssT1I CCWWGG 1 cut(s) 409
Bst2UI CCWGG 3 cut(s) 10, 211, 456
Bst4CI ACNGT 2 cut(s) 193, 343
BstBAI YACGTR 1 cut(s) 61
BstC8I GCNNGC 3 cut(s) 6, 151, 317
BstDEI CTNAG 1 cut(s) 310
BstDSI CCRYGG 1 cut(s) 320
BstF5I GGATG 2 cut(s) 330, 518
BstH2I RGCGCY 1 cut(s) 462
BstHHI GCGC 2 cut(s) 31, 461
BstKTI GATC 2 cut(s) 226, 231
BstMBI GATC 2 cut(s) 223, 228
BstMCI CGRYCG 1 cut(s) 320
BstMWI GCNNNNNNNGC 2 cut(s) 10, 312
BstNI CCWGG 3 cut(s) 10, 211, 456
BstNSI RCATGY 1 cut(s) 153
BstPAI GACNNNNGTC 1 cut(s) 344
BstSCI CCNGG 4 cut(s) 8, 178, 209, 454
BstSFI CTRYAG 1 cut(s) 563
BstSLI GKGCMC 1 cut(s) 66
BstV1I GCAGC 1 cut(s) 549
BstX2I RGATCY 1 cut(s) 223
BstYI RGATCY 1 cut(s) 223
BstZI CGGCCG 1 cut(s) 317
Bsu15I ATCGAT 1 cut(s) 35
BsuRI GGCC 3 cut(s) 8, 315, 319
BsuTUI ATCGAT 1 cut(s) 35
BtgI CCRYGG 1 cut(s) 320
BtsCI GGATG 2 cut(s) 330, 518
BtsIMutI CAGTG 2 cut(s) 274, 535
Cac8I GCNNGC 3 cut(s) 6, 151, 317
CfoI GCGC 2 cut(s) 31, 461
Cfr10I RCCGGY 1 cut(s) 315
Cfr13I GGNCC 1 cut(s) 406
ClaI ATCGAT 1 cut(s) 35
Csp6I GTAC 2 cut(s) 427, 439
CviAII CATG 3 cut(s) 150, 244, 497
CviJI RGCY 8 cut(s) 8, 13, 41, 266, 306, 315, 319, 562
CviKI_1 RGCY 8 cut(s) 8, 13, 41, 266, 306, 315, 319, 562
CviQI GTAC 2 cut(s) 427, 439
DdeI CTNAG 1 cut(s) 310
DpnI GATC 2 cut(s) 225, 230
DpnII GATC 2 cut(s) 223, 228
DrdI GACNNNNNNGTC 1 cut(s) 397
DseDI GACNNNNNNGTC 1 cut(s) 397
EaeI YGGCCR 1 cut(s) 317
EagI CGGCCG 1 cut(s) 317
EclXI CGGCCG 1 cut(s) 317
Eco130I CCWWGG 1 cut(s) 409
Eco147I AGGCCT 1 cut(s) 8
Eco47I GGWCC 1 cut(s) 406
Eco52I CGGCCG 1 cut(s) 317
Eco57I CTGAAG 1 cut(s) 371
EcoO109I RGGNCCY 1 cut(s) 406
EcoRII CCWGG 3 cut(s) 8, 209, 454
EcoT14I CCWWGG 1 cut(s) 409
EcoT22I ATGCAT 1 cut(s) 520
ErhI CCWWGG 1 cut(s) 409
FaeI CATG 3 cut(s) 153, 247, 500
FaqI GGGAC 4 cut(s) 195, 227, 419, 478
FatI CATG 3 cut(s) 149, 243, 496
FauNDI CATATG 1 cut(s) 119
FbaI TGATCA 1 cut(s) 228
Fnu4HI GCNGC 3 cut(s) 14, 132, 563
FokI GGATG 2 cut(s) 337, 505
FseI GGCCGGCC 1 cut(s) 319
Fsp4HI GCNGC 3 cut(s) 14, 132, 563
FspBI CTAG 2 cut(s) 221, 410
GlaI GCGC 2 cut(s) 30, 460
GluI GCNGC 3 cut(s) 14, 132, 563
GsaI CCCAGC 1 cut(s) 465
HaeII RGCGCY 1 cut(s) 462
HaeIII GGCC 3 cut(s) 8, 315, 319
HapII CCGG 2 cut(s) 179, 316
HhaI GCGC 2 cut(s) 31, 461
Hin1II CATG 3 cut(s) 153, 247, 500
Hin6I GCGC 2 cut(s) 29, 459
HinP1I GCGC 2 cut(s) 29, 459
HinfI GANTC 1 cut(s) 237
HpaII CCGG 2 cut(s) 179, 316
HphI GGTGA 2 cut(s) 190, 563
Hpy166II GTNNAC 4 cut(s) 64, 390, 481, 570
Hpy188I TCNGA 4 cut(s) 74, 104, 228, 599
Hpy188III TCNNGA 1 cut(s) 349
Hpy8I GTNNAC 4 cut(s) 64, 390, 481, 570
HpyAV CCTTC 1 cut(s) 584
HpyCH4III ACNGT 2 cut(s) 193, 343
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 9 cut(s) 4, 16, 64, 153, 247, 280, 518, 535, 565
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 312
HpyF3I CTNAG 1 cut(s) 310
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 3 cut(s) 153, 247, 500
HspAI GCGC 2 cut(s) 29, 459
KroI GCCGGC 1 cut(s) 315
KroNI GCCGGC 1 cut(s) 317
Ksp22I TGATCA 1 cut(s) 228
Kzo9I GATC 2 cut(s) 223, 228
LmnI GCTCC 1 cut(s) 260
Lsp1109I GCAGC 1 cut(s) 549
LweI GCATC 2 cut(s) 505, 527
MaeI CTAG 2 cut(s) 221, 410
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 1 cut(s) 125
MalI GATC 2 cut(s) 225, 230
MboI GATC 2 cut(s) 223, 228
MflI RGATCY 1 cut(s) 223
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 6 cut(s) 17, 77, 286, 394, 445, 523
MmeI TCCRAC 1 cut(s) 396
Mph1103I ATGCAT 1 cut(s) 520
MroNI GCCGGC 1 cut(s) 315
MseI TTAA 1 cut(s) 363
MslI CAYNNNNRTG 1 cut(s) 158
MspI CCGG 2 cut(s) 179, 316
MspR9I CCNGG 4 cut(s) 10, 180, 211, 456
MvaI CCWGG 3 cut(s) 10, 211, 456
MwoI GCNNNNNNNGC 2 cut(s) 10, 312
NaeI GCCGGC 1 cut(s) 317
NciI CCSGG 1 cut(s) 180
NdeI CATATG 1 cut(s) 119
NdeII GATC 2 cut(s) 223, 228
NgoMIV GCCGGC 1 cut(s) 315
NlaIII CATG 3 cut(s) 153, 247, 500
NlaIV GGNNCC 2 cut(s) 106, 407
NsiI ATGCAT 1 cut(s) 520
NspI RCATGY 1 cut(s) 153
OliI CACNNNNGTG 1 cut(s) 158
PaeI GCATGC 1 cut(s) 153
PceI AGGCCT 1 cut(s) 8
PdiI GCCGGC 1 cut(s) 317
PfeI GAWTC 1 cut(s) 237
PkrI GCNGC 3 cut(s) 15, 133, 564
Ppu21I YACGTR 1 cut(s) 61
PpuMI RGGWCCY 1 cut(s) 406
PshAI GACNNNNGTC 1 cut(s) 344
Psp5II RGGWCCY 1 cut(s) 406
Psp6I CCWGG 3 cut(s) 8, 209, 454
PspFI CCCAGC 1 cut(s) 461
PspGI CCWGG 3 cut(s) 8, 209, 454
PspN4I GGNNCC 2 cut(s) 106, 407
PspPI GGNCC 1 cut(s) 406
PspPPI RGGWCCY 1 cut(s) 406
PstI CTGCAG 1 cut(s) 567
PsuI RGATCY 1 cut(s) 223
RigI GGCCGGCC 1 cut(s) 319
RsaI GTAC 2 cut(s) 428, 440
RsaNI GTAC 2 cut(s) 427, 439
RseI CAYNNNNRTG 1 cut(s) 158
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 3 cut(s) 14, 132, 563
Sau3AI GATC 2 cut(s) 223, 228
Sau96I GGNCC 1 cut(s) 406
ScrFI CCNGG 4 cut(s) 10, 180, 211, 456
SduI GDGCHC 1 cut(s) 66
SetI ASST 3 cut(s) 63, 308, 411
SfaNI GCATC 2 cut(s) 505, 527
SfcI CTRYAG 1 cut(s) 563
SinI GGWCC 1 cut(s) 406
SmiMI CAYNNNNRTG 1 cut(s) 158
SphI GCATGC 1 cut(s) 153
Sse9I AATT 6 cut(s) 17, 77, 286, 394, 445, 523
SseBI AGGCCT 1 cut(s) 8
SsiI CCGC 1 cut(s) 132
SspMI CTAG 2 cut(s) 221, 410
StuI AGGCCT 1 cut(s) 8
StyD4I CCNGG 4 cut(s) 8, 178, 209, 454
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 2 cut(s) 193, 343
TaiI ACGT 1 cut(s) 63
TaqI TCGA 1 cut(s) 35
TasI AATT 6 cut(s) 17, 77, 286, 394, 445, 523
TatI WGTACW 2 cut(s) 426, 438
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 1 cut(s) 237
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TscAI CASTG 2 cut(s) 274, 535
TseI GCWGC 2 cut(s) 13, 562
TspDTI ATGAA 1 cut(s) 570
TspGWI ACGGA 2 cut(s) 99, 482
TspRI CASTG 2 cut(s) 274, 535
VneI GTGCAC 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 406
XceI RCATGY 1 cut(s) 153
XmaJI CCTAGG 1 cut(s) 409
XspI CTAG 2 cut(s) 221, 410
Zsp2I ATGCAT 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.