MD02G1104400.v1.1

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014883
Physical Location & Seq
Reverse (-)
8385483 .. 8387051
1569 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1104400.v1.1.491

Sequence Viewer

Length: 693 bp
ATGACTTATGGATTACGTCAGAGATTAAGGTGGCTTTACGATAGGACGATTATACATGTCAAAGAAGGGGTTTATGCAGAAAATGTGGAGATTCCGAGCTACAAGACCAACCTTGTTCTTTTTGGAGATGGAAGTGACATCACTTTCATCACTGGTAAACGCAGTTATTATGATGGATGGACTCCATTCAGATCTGCAACCGTAGCTGTGTCCGGAGAGGGCTTTCTAGCAAGAGACATAACAATTAAGGACACGGCAGGGCCAGAAAAGCACCAAGCAGTTGCTCTGAGAATTAATGCAGACCTGGCTGCACATTACAAATGCGCCATCGATGGCTACCAAGACACATTATACGTGCACTCTTTCAGACAATTCTATAGAGAGTGCGATATATTCGGAACCATAGGCTACATATGTGGTACCGCCGCAGTTGTATTTCAAGCATGCAACATTGTGTCCAAGATACCAATGCCAGGACAAATCACAGTCGTCACGGCGCAGTCCAGGGACACACTAGATCAGAACACAGGAATCTCCATGCCGAACTGCTACATTCTAGCCAGTGATGAGTTGCACTCTCATTCCAGCATTGTCAAAAGCTACTTAGGCCGGCCGTGGAGGAATGACTCTACGACTGTTGTCCTGAAATCCTACATTAACGACTTTATTGACCCGACTGGGTGCACTAATTAG

Protein Analysis

231

Amino Acids

25.73

Weight (kDa)

6.58

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 14 - 228 1.4e-93 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014883)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26440
fragaria_vesca FvH4_1g09580
malus_domestica MD02G1104400.v1.1 MD02G1104500.v1.1 MD15G1224800.v1.1
prunus_persica Prupe.7G190400_v2.0.a1
pyrus_communis pycom02g08230
rosa_chinensis RchiOBHm_Chr2g0096441
rosa_laevigata RLG00000016640
rosa_multiflora Rmu_sc0004096.1_g000025
rosa_roxburghii Rroxscaffold_2G00145650
rosa_samantha Rh2AG104700 Rh2BG106900 Rh2CG108700 Rh2DG107800
rosa_wichuraiana Rw2G008090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 419
AccB1I GGYRCC 1 cut(s) 419
AccIII TCCGGA 1 cut(s) 212
AciI CCGC 2 cut(s) 423, 426
AcoI YGGCCR 1 cut(s) 611
AfaI GTAC 1 cut(s) 421
AfiI CCNNNNNNNGG 1 cut(s) 473
AflIII ACRYGT 1 cut(s) 55
AgsI TTSAA 1 cut(s) 440
AjnI CCWGG 3 cut(s) 303, 472, 503
AluBI AGCT 3 cut(s) 99, 206, 600
AluI AGCT 3 cut(s) 99, 206, 600
Alw21I GWGCWC 2 cut(s) 360, 686
Alw26I GTCTC 1 cut(s) 228
Alw44I GTGCAC 2 cut(s) 356, 682
Aor13HI TCCGGA 1 cut(s) 212
AoxI GGCC 3 cut(s) 260, 607, 611
ApaLI GTGCAC 2 cut(s) 356, 682
ApeKI GCWGC 1 cut(s) 308
AseI ATTAAT 1 cut(s) 294
Asp718I GGTACC 1 cut(s) 419
AspLEI GCGC 2 cut(s) 326, 499
AspS9I GGNCC 1 cut(s) 260
BaeGI GKGCMC 2 cut(s) 360, 686
BanI GGYRCC 1 cut(s) 419
Bbv12I GWGCWC 2 cut(s) 360, 686
BbvI GCAGC 1 cut(s) 295
BccI CCATC 5 cut(s) 122, 167, 171, 326, 335
BceAI ACGGC 3 cut(s) 270, 510, 598
BciT130I CCWGG 3 cut(s) 305, 474, 505
BcoDI GTCTC 1 cut(s) 228
BfaI CTAG 3 cut(s) 227, 515, 557
BfmI CTRYAG 1 cut(s) 376
BglII AGATCT 1 cut(s) 191
BisI GCNGC 2 cut(s) 309, 426
BlsI GCNGC 2 cut(s) 310, 427
Bme1390I CCNGG 3 cut(s) 305, 474, 505
BmgT120I GGNCC 1 cut(s) 260
BmiI GGNNCC 2 cut(s) 400, 421
BmrFI CCNGG 3 cut(s) 305, 474, 505
BmrI ACTGGG 1 cut(s) 687
BmuI ACTGGG 1 cut(s) 687
BoxI GACNNNNGTC 1 cut(s) 638
BplI GAGNNNNNCTC 2 cut(s) 560, 592
Bsa29I ATCGAT 1 cut(s) 330
BsaAI YACGTR 1 cut(s) 355
BsaJI CCNNGG 2 cut(s) 504, 614
BsaWI WCCGGW 1 cut(s) 212
Bsc4I CCNNNNNNNGG 1 cut(s) 473
Bse118I RCCGGY 1 cut(s) 609
Bse1I ACTGG 3 cut(s) 157, 561, 682
BseAI TCCGGA 1 cut(s) 212
BseBI CCWGG 3 cut(s) 305, 474, 505
BseCI ATCGAT 1 cut(s) 330
BseDI CCNNGG 2 cut(s) 504, 614
BseGI GGATG 1 cut(s) 182
BseLI CCNNNNNNNGG 1 cut(s) 473
BseMII CTCAG 1 cut(s) 278
BseNI ACTGG 3 cut(s) 157, 561, 682
BseSI GKGCMC 2 cut(s) 360, 686
BseX3I CGGCCG 1 cut(s) 611
BseXI GCAGC 1 cut(s) 295
BsgI GTGCAG 1 cut(s) 294
Bsh1285I CGRYCG 1 cut(s) 614
BshFI GGCC 3 cut(s) 262, 609, 613
BshNI GGYRCC 1 cut(s) 419
BshVI ATCGAT 1 cut(s) 330
BsiEI CGRYCG 1 cut(s) 614
BsiHKAI GWGCWC 2 cut(s) 360, 686
BsiSI CCGG 2 cut(s) 213, 610
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 1 cut(s) 473
BsmAI GTCTC 1 cut(s) 228
BsmFI GGGAC 1 cut(s) 521
BsnI GGCC 3 cut(s) 262, 609, 613
Bsp1286I GDGCHC 2 cut(s) 360, 686
Bsp13I TCCGGA 1 cut(s) 212
Bsp143I GATC 2 cut(s) 191, 517
BspACI CCGC 2 cut(s) 423, 426
BspANI GGCC 3 cut(s) 262, 609, 613
BspCNI CTCAG 1 cut(s) 279
BspDI ATCGAT 1 cut(s) 330
BspEI TCCGGA 1 cut(s) 212
BspLI GGNNCC 2 cut(s) 400, 421
BspT107I GGYRCC 1 cut(s) 419
BsrFI RCCGGY 1 cut(s) 609
BsrI ACTGG 3 cut(s) 157, 561, 682
BssAI RCCGGY 1 cut(s) 609
BssECI CCNNGG 2 cut(s) 504, 614
BssMI GATC 2 cut(s) 191, 517
Bst2UI CCWGG 3 cut(s) 305, 474, 505
Bst4CI ACNGT 3 cut(s) 202, 487, 637
BstBAI YACGTR 1 cut(s) 355
BstC8I GCNNGC 2 cut(s) 445, 611
BstDEI CTNAG 2 cut(s) 287, 604
BstDSI CCRYGG 1 cut(s) 614
BstF5I GGATG 1 cut(s) 182
BstHHI GCGC 2 cut(s) 326, 499
BstKTI GATC 2 cut(s) 194, 520
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 2 cut(s) 191, 517
BstMCI CGRYCG 1 cut(s) 614
BstMWI GCNNNNNNNGC 4 cut(s) 203, 268, 305, 606
BstNI CCWGG 3 cut(s) 305, 474, 505
BstNSI RCATGY 2 cut(s) 59, 447
BstPAI GACNNNNGTC 1 cut(s) 638
BstSCI CCNGG 3 cut(s) 303, 472, 503
BstSFI CTRYAG 1 cut(s) 376
BstSLI GKGCMC 2 cut(s) 360, 686
BstV1I GCAGC 1 cut(s) 295
BstX2I RGATCY 1 cut(s) 191
BstYI RGATCY 1 cut(s) 191
BstZI CGGCCG 1 cut(s) 611
Bsu15I ATCGAT 1 cut(s) 330
BsuRI GGCC 3 cut(s) 262, 609, 613
BsuTUI ATCGAT 1 cut(s) 330
BtgI CCRYGG 1 cut(s) 614
BtsCI GGATG 1 cut(s) 182
BtsIMutI CAGTG 2 cut(s) 150, 568
Cac8I GCNNGC 2 cut(s) 445, 611
CfoI GCGC 2 cut(s) 326, 499
Cfr10I RCCGGY 1 cut(s) 609
Cfr13I GGNCC 1 cut(s) 260
ClaI ATCGAT 1 cut(s) 330
Csp6I GTAC 1 cut(s) 420
CviAII CATG 3 cut(s) 56, 444, 538
CviQI GTAC 1 cut(s) 420
DdeI CTNAG 2 cut(s) 287, 604
DpnI GATC 2 cut(s) 193, 519
DpnII GATC 2 cut(s) 191, 517
EaeI YGGCCR 1 cut(s) 611
EagI CGGCCG 1 cut(s) 611
EclXI CGGCCG 1 cut(s) 611
Eco52I CGGCCG 1 cut(s) 611
EcoRII CCWGG 3 cut(s) 303, 472, 503
FaeI CATG 3 cut(s) 59, 447, 541
FaqI GGGAC 1 cut(s) 521
FatI CATG 3 cut(s) 55, 443, 537
FauNDI CATATG 1 cut(s) 413
Fnu4HI GCNGC 2 cut(s) 309, 426
FokI GGATG 1 cut(s) 189
FseI GGCCGGCC 1 cut(s) 613
Fsp4HI GCNGC 2 cut(s) 309, 426
FspBI CTAG 3 cut(s) 227, 515, 557
GlaI GCGC 2 cut(s) 325, 498
GluI GCNGC 2 cut(s) 309, 426
HaeIII GGCC 3 cut(s) 262, 609, 613
HapII CCGG 2 cut(s) 213, 610
HhaI GCGC 2 cut(s) 326, 499
Hin1II CATG 3 cut(s) 59, 447, 541
Hin6I GCGC 2 cut(s) 324, 497
HinP1I GCGC 2 cut(s) 324, 497
HinfI GANTC 4 cut(s) 91, 181, 531, 626
HpaII CCGG 2 cut(s) 213, 610
Hpy166II GTNNAC 3 cut(s) 158, 358, 684
Hpy188I TCNGA 7 cut(s) 21, 96, 191, 288, 368, 398, 522
Hpy188III TCNNGA 2 cut(s) 213, 643
Hpy8I GTNNAC 3 cut(s) 158, 358, 684
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 3 cut(s) 202, 487, 637
HpyCH4IV ACGT 2 cut(s) 16, 354
HpyCH4V TGCA 8 cut(s) 77, 197, 299, 311, 358, 447, 574, 684
HpyF10VI GCNNNNNNNGC 4 cut(s) 203, 268, 305, 606
HpyF3I CTNAG 2 cut(s) 287, 604
HpySE526I ACGT 2 cut(s) 16, 354
Hsp92II CATG 3 cut(s) 59, 447, 541
HspAI GCGC 2 cut(s) 324, 497
Kpn2I TCCGGA 1 cut(s) 212
KpnI GGTACC 1 cut(s) 423
KroI GCCGGC 1 cut(s) 609
KroNI GCCGGC 1 cut(s) 611
Kzo9I GATC 2 cut(s) 191, 517
Lsp1109I GCAGC 1 cut(s) 295
MaeI CTAG 3 cut(s) 227, 515, 557
MaeII ACGT 2 cut(s) 16, 354
MaeIII GTNAC 2 cut(s) 134, 490
MalI GATC 2 cut(s) 193, 519
MboI GATC 2 cut(s) 191, 517
MflI RGATCY 1 cut(s) 191
MhlI GDGCHC 2 cut(s) 360, 686
MluCI AATT 4 cut(s) 243, 291, 371, 688
MlyI GAGTC 2 cut(s) 175, 620
MnlI CCTC 2 cut(s) 211, 612
MroI TCCGGA 1 cut(s) 212
MroNI GCCGGC 1 cut(s) 609
MseI TTAA 4 cut(s) 26, 246, 294, 657
MspI CCGG 2 cut(s) 213, 610
MspR9I CCNGG 3 cut(s) 305, 474, 505
MvaI CCWGG 3 cut(s) 305, 474, 505
MwoI GCNNNNNNNGC 4 cut(s) 203, 268, 305, 606
NaeI GCCGGC 1 cut(s) 611
NdeI CATATG 1 cut(s) 413
NdeII GATC 2 cut(s) 191, 517
NgoMIV GCCGGC 1 cut(s) 609
NlaIII CATG 3 cut(s) 59, 447, 541
NlaIV GGNNCC 2 cut(s) 400, 421
NmuCI GTSAC 2 cut(s) 134, 490
NspI RCATGY 2 cut(s) 59, 447
PaeI GCATGC 1 cut(s) 447
PciI ACATGT 1 cut(s) 55
PdiI GCCGGC 1 cut(s) 611
PfeI GAWTC 2 cut(s) 91, 531
PkrI GCNGC 2 cut(s) 310, 427
PleI GAGTC 2 cut(s) 175, 620
PpsI GAGTC 2 cut(s) 175, 620
Ppu21I YACGTR 1 cut(s) 355
PscI ACATGT 1 cut(s) 55
PshAI GACNNNNGTC 1 cut(s) 638
PshBI ATTAAT 1 cut(s) 294
Psp6I CCWGG 3 cut(s) 303, 472, 503
PspGI CCWGG 3 cut(s) 303, 472, 503
PspN4I GGNNCC 2 cut(s) 400, 421
PspPI GGNCC 1 cut(s) 260
PsuI RGATCY 1 cut(s) 191
RigI GGCCGGCC 1 cut(s) 613
RsaI GTAC 1 cut(s) 421
RsaNI GTAC 1 cut(s) 420
SaqAI TTAA 4 cut(s) 26, 246, 294, 657
SatI GCNGC 2 cut(s) 309, 426
Sau3AI GATC 2 cut(s) 191, 517
Sau96I GGNCC 1 cut(s) 260
SchI GAGTC 2 cut(s) 175, 620
ScrFI CCNGG 3 cut(s) 305, 474, 505
SduI GDGCHC 2 cut(s) 360, 686
SetI ASST 8 cut(s) 19, 32, 101, 114, 208, 306, 357, 602
SfcI CTRYAG 1 cut(s) 376
SphI GCATGC 1 cut(s) 447
Sse9I AATT 4 cut(s) 243, 291, 371, 688
SsiI CCGC 2 cut(s) 423, 426
SspMI CTAG 3 cut(s) 227, 515, 557
StyD4I CCNGG 3 cut(s) 303, 472, 503
TaaI ACNGT 3 cut(s) 202, 487, 637
TaiI ACGT 2 cut(s) 19, 357
TaqI TCGA 1 cut(s) 330
TasI AATT 4 cut(s) 243, 291, 371, 688
TauI GCSGC 1 cut(s) 428
TfiI GAWTC 2 cut(s) 91, 531
Tru1I TTAA 4 cut(s) 26, 246, 294, 657
Tru9I TTAA 4 cut(s) 26, 246, 294, 657
TscAI CASTG 2 cut(s) 157, 568
TseFI GTSAC 2 cut(s) 134, 490
TseI GCWGC 1 cut(s) 308
Tsp45I GTSAC 2 cut(s) 134, 490
TspDTI ATGAA 1 cut(s) 136
TspRI CASTG 2 cut(s) 157, 568
VneI GTGCAC 2 cut(s) 356, 682
VspI ATTAAT 1 cut(s) 294
XceI RCATGY 2 cut(s) 59, 447
XspI CTAG 3 cut(s) 227, 515, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.