FvH4_1g10671

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0015461
Physical Location & Seq
Forward (+)
5842094 .. 5843446
1353 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g10671.t1

Sequence Viewer

Length: 1353 bp
ATGGGAAAGATGTCCCCCTCAGTCCGTTCCCTAGTCTCAACCTCCCTCATCAAAAAGCCACCTTCTTCATCCCCATCTCATAAACCCCACACCCACCTCACCGGAAAACCCCCACCTCAAAATTCCGGCAAAACCCAAACCCAGACCCACAACCCATTCACCTCCCCAAACCTCTCAGACGCCAAAAGGGTCTTCAATTCCATCGCCACCTCAACAAGAAACCCTCTTGATCTTCGCCGCCACAACGCCCTCCTCCAATCCTACGCCGCAATCTCCACCGTCAACGACTCCATCGCTTTCTTCAACCACATGATCAAAACCCAGCCGGCATTCTCGCCGGAGCGGTCCACCTATCACATCCTGCTCGCCCAGTCCTGCACTGTACCTGATCAGACCCTCCAGTCTGTTCACCAAGTTCTCAACTTTATGGTCAACAATGGCTTCCACCCAGATAAGGTCACCACCGACATTGCCGTCCGGTCACTCTGCCTTGCCGGGCACGTCGACGAGGCAGTGCAGGTGATGAAGCAAATGGCGTTGAAAAGCACGGTACCGGATGCTTATACTTATAACTTTCTTGTCAAGTGTCTATGCAAGTCGAGAGTTTTGAGTACTGTGAGAGGTTTTATTGATGAAATGAAGTCTTTTGGTTTGAAGCCGGATTTGGTTACATTTACGATTATGATTGACAATGTAGCTAATAGCAAGAATTTACGTGAGGCTACTCGATTAGTGGACGAGTTGGAGAAGGAGGGGTTTAAGCCGGATTGCTATGTGTATAATACGATTATGAAGGGTTATTGTATGCTGAGTAAGGGGAATGAGGTGGTTCAGGTGTTGAAGGAGATGCAGGCGAAGGGAGTGGAGCCTGATCTTGTTACATATAACACTCTGATTTTTGGGTTGTCGAAGTGTGGCAGAGTTATGGACGCGAAGAAGTATTTGTGGATCATGGTGGAAATGGGGCATTTCCCCGATGAGGTGACTTATACTTCGTTGATGAATGGATTGTGTCGGAAAGGGGATGCATTGGGTGCAGTGGCATTGTTGTCCGAGATGCAAGCGCAGGGGTGCAGGCCGAATGAGTGCACCTATAATACGTTGCTTCATGGGTTGTGTAAGGGAAGGTTGTTAGAGAAAGGGCTCGAATTGTATGGACTGATGAAAAAAGATGGGATGAAGCTTGACACTGCTTCGTATGGTACGCTTGTGAGGACACTTTGTAGGGCAGGCAGGATTGCAGATGCTTATGAAGTGTTTGATTATGCAGTTGAGAGTAAGAGCTTGACAAATGTTTCTGCTTACTTGACTTTGGAAGCTACAGTTAAGCAGGTTAAGAAAGCCCGACCTTGA

Protein Analysis

451

Amino Acids

49.95

Weight (kDa)

9.13

Isoelectric Point (pI)

33.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 146 - 178 8.1e-06 PPR repeat
PPR_2 PF13041 161 - 199 1.3e-08 PPR repeat family
PPR_long PF17177 171 - 309 2.6e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 210 - 267 5.3e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 234 - 268 2e-06 PPR repeat family
PPR_3 PF13812 243 - 299 1.7e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 255 - 304 4.9e-14 PPR repeat family
PPR_1 PF12854 286 - 313 2.9e-09 PPR repeat
PPR_2 PF13041 290 - 339 3e-14 PPR repeat family
PPR_long PF17177 318 - 425 2.2e-09 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 321 - 354 2.4e-10 PPR repeat
PPR_3 PF13812 324 - 370 1.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 325 - 374 5.8e-18 PPR repeat family
PPR PF01535 328 - 358 4.1e-07 PPR repeat
PPR_1 PF12854 357 - 388 4.2e-08 PPR repeat
PPR_2 PF13041 363 - 409 9e-11 PPR repeat family
PPR PF01535 364 - 392 6.2e-07 PPR repeat
PPR_1 PF12854 391 - 422 4.6e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015461)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17670 AT2G17670
fragaria_vesca FvH4_1g10671
malus_domestica MD02G1120600.v1.1
prunus_persica Prupe.7G178400_v2.0.a1
rosa_laevigata RLG00000016739
rosa_multiflora Rmu_co8429607.1_g000001 Rmu_sc0000974.1_g000027 Rmu_sc0008442.1_g000010
rosa_roxburghii Rroxscaffold_6G00394240
rosa_rugosa Rorug02G0068500
rosa_samantha Rh2AG115200 Rh2BG118100 Rh2CG119700 Rh2DG119400
rosa_wichuraiana Rw2G009000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 570
AarI CACCTGC 1 cut(s) 508
AasI GACNNNNNNGTC 2 cut(s) 400, 473
Acc36I ACCTGC 2 cut(s) 508, 1321
Acc65I GGTACC 1 cut(s) 550
AccB1I GGYRCC 1 cut(s) 550
AccBSI CCGCTC 1 cut(s) 343
AccI GTMKAC 1 cut(s) 504
AccII CGCG 1 cut(s) 932
AciI CCGC 3 cut(s) 238, 267, 343
AclWI GGATC 1 cut(s) 956
AcsI RAATTY 2 cut(s) 121, 709
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 4 cut(s) 384, 552, 613, 1204
AfiI CCNNNNNNNGG 2 cut(s) 454, 979
AgsI TTSAA 5 cut(s) 196, 304, 541, 655, 841
AjiI CACGTC 1 cut(s) 502
AjuI GAANNNNNNNTTGG 4 cut(s) 176, 208, 647, 679
AluBI AGCT 4 cut(s) 698, 1183, 1284, 1319
AluI AGCT 4 cut(s) 698, 1183, 1284, 1319
Alw21I GWGCWC 1 cut(s) 1091
Alw26I GTCTC 1 cut(s) 40
Alw44I GTGCAC 1 cut(s) 1087
AlwI GGATC 1 cut(s) 956
AoxI GGCC 1 cut(s) 1076
ApaLI GTGCAC 1 cut(s) 1087
ApoI RAATTY 2 cut(s) 121, 709
ArsI GACNNNNNNTTYG 2 cut(s) 976, 1008
Asp718I GGTACC 1 cut(s) 550
AspLEI GCGC 1 cut(s) 1066
AspS9I GGNCC 1 cut(s) 345
AsuC2I CCSGG 1 cut(s) 496
AsuHPI GGTGA 6 cut(s) 91, 151, 401, 451, 532, 994
AvaII GGWCC 1 cut(s) 345
BaeGI GKGCMC 2 cut(s) 501, 1091
BaeI ACNNNNGTAYC 2 cut(s) 1194, 1227
BanI GGYRCC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 1146
BbsI GAAGAC 1 cut(s) 184
Bbv12I GWGCWC 1 cut(s) 1091
BccI CCATC 4 cut(s) 82, 209, 299, 1166
BceAI ACGGC 1 cut(s) 458
BclI TGATCA 2 cut(s) 312, 388
BcnI CCSGG 1 cut(s) 496
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 1320
BfuAI ACCTGC 2 cut(s) 508, 1321
BisI GCNGC 2 cut(s) 238, 267
BlsI GCNGC 2 cut(s) 239, 268
BmcAI AGTACT 1 cut(s) 613
Bme1390I CCNGG 1 cut(s) 496
Bme18I GGWCC 1 cut(s) 345
BmgBI CACGTC 1 cut(s) 502
BmgT120I GGNCC 1 cut(s) 345
BmiI GGNNCC 2 cut(s) 552, 867
BmrFI CCNGG 1 cut(s) 496
BmrI ACTGGG 1 cut(s) 364
BmsI GCATC 5 cut(s) 547, 837, 1015, 1047, 1234
BmuI ACTGGG 1 cut(s) 364
BpiI GAAGAC 1 cut(s) 184
BpmI CTGGAG 1 cut(s) 383
BpuMI CCSGG 1 cut(s) 496
BsaAI YACGTR 1 cut(s) 716
BsaHI GRCGYC 1 cut(s) 180
BsaWI WCCGGW 3 cut(s) 101, 477, 553
Bsc4I CCNNNNNNNGG 2 cut(s) 454, 979
Bse118I RCCGGY 1 cut(s) 325
Bse1I ACTGG 2 cut(s) 370, 400
Bse3DI GCAATG 1 cut(s) 468
BseGI GGATG 5 cut(s) 68, 357, 562, 1030, 1182
BseLI CCNNNNNNNGG 2 cut(s) 454, 979
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 3 cut(s) 33, 189, 800
BseNI ACTGG 2 cut(s) 370, 400
BseRI GAGGAG 1 cut(s) 242
BseSI GKGCMC 2 cut(s) 501, 1091
BseYI CCCAGC 1 cut(s) 321
BsgI GTGCAG 4 cut(s) 361, 536, 1056, 1093
Bsh1236I CGCG 1 cut(s) 932
BshFI GGCC 1 cut(s) 1078
BshNI GGYRCC 1 cut(s) 550
BsiHKAI GWGCWC 1 cut(s) 1091
BsiSI CCGG 9 cut(s) 102, 126, 326, 338, 478, 495, 554, 659, 764
BslI CCNNNNNNNGG 2 cut(s) 454, 979
BsmAI GTCTC 1 cut(s) 40
BsmI GAATGC 1 cut(s) 329
BsnI GGCC 1 cut(s) 1078
Bsp1286I GDGCHC 3 cut(s) 501, 1091, 1146
Bsp143I GATC 5 cut(s) 229, 312, 388, 871, 948
BspACI CCGC 3 cut(s) 238, 267, 343
BspANI GGCC 1 cut(s) 1078
BspCNI CTCAG 3 cut(s) 32, 188, 801
BspFNI CGCG 1 cut(s) 932
BspLI GGNNCC 2 cut(s) 552, 867
BspMI ACCTGC 2 cut(s) 508, 1321
BspPI GGATC 1 cut(s) 956
BspT107I GGYRCC 1 cut(s) 550
BsrBI CCGCTC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 468
BsrFI RCCGGY 1 cut(s) 325
BsrI ACTGG 2 cut(s) 370, 400
BssAI RCCGGY 1 cut(s) 325
BssMI GATC 5 cut(s) 229, 312, 388, 871, 948
BssNI GRCGYC 1 cut(s) 180
Bst4CI ACNGT 5 cut(s) 280, 382, 550, 616, 1324
BstACI GRCGYC 1 cut(s) 180
BstAPI GCANNNNNTGC 1 cut(s) 1034
BstBAI YACGTR 1 cut(s) 716
BstC8I GCNNGC 6 cut(s) 327, 366, 852, 1062, 1076, 1231
BstDEI CTNAG 3 cut(s) 19, 175, 809
BstEII GGTNACC 1 cut(s) 457
BstF5I GGATG 5 cut(s) 68, 357, 562, 1030, 1182
BstFNI CGCG 1 cut(s) 932
BstHHI GCGC 1 cut(s) 1066
BstKTI GATC 5 cut(s) 232, 315, 391, 874, 951
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 5 cut(s) 229, 312, 388, 871, 948
BstMWI GCNNNNNNNGC 1 cut(s) 1034
BstPI GGTNACC 1 cut(s) 457
BstSCI CCNGG 1 cut(s) 494
BstSFI CTRYAG 1 cut(s) 1320
BstSLI GKGCMC 2 cut(s) 501, 1091
BstUI CGCG 1 cut(s) 932
BstV2I GAAGAC 1 cut(s) 184
BsuRI GGCC 1 cut(s) 1078
BtgZI GCGATG 2 cut(s) 187, 277
BtrI CACGTC 1 cut(s) 502
BtsCI GGATG 5 cut(s) 68, 357, 562, 1030, 1182
BtsI GCAGTG 3 cut(s) 519, 1044, 1188
BtsIMutI CAGTG 4 cut(s) 378, 519, 1044, 1188
BveI ACCTGC 2 cut(s) 508, 1321
Cac8I GCNNGC 6 cut(s) 327, 366, 852, 1062, 1076, 1231
CfoI GCGC 1 cut(s) 1066
Cfr10I RCCGGY 1 cut(s) 325
Cfr13I GGNCC 1 cut(s) 345
CseI GACGC 2 cut(s) 188, 938
Csp6I GTAC 4 cut(s) 383, 551, 612, 1203
CspCI CAANNNNNGTGG 2 cut(s) 451, 486
CviAII CATG 3 cut(s) 310, 952, 1109
CviQI GTAC 4 cut(s) 383, 551, 612, 1203
DdeI CTNAG 3 cut(s) 19, 175, 809
DpnI GATC 5 cut(s) 231, 314, 390, 873, 950
DpnII GATC 5 cut(s) 229, 312, 388, 871, 948
DrdI GACNNNNNNGTC 2 cut(s) 400, 473
DseDI GACNNNNNNGTC 2 cut(s) 400, 473
Eco24I GRGCYC 1 cut(s) 1146
Eco47I GGWCC 1 cut(s) 345
Eco91I GGTNACC 1 cut(s) 457
EcoO65I GGTNACC 1 cut(s) 457
EcoT22I ATGCAT 1 cut(s) 1030
EcoT38I GRGCYC 1 cut(s) 1146
FaeI CATG 3 cut(s) 313, 955, 1112
FatI CATG 3 cut(s) 309, 951, 1108
FbaI TGATCA 2 cut(s) 312, 388
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 2 cut(s) 238, 267
FokI GGATG 5 cut(s) 55, 344, 569, 1037, 1189
FriOI GRGCYC 1 cut(s) 1146
Fsp4HI GCNGC 2 cut(s) 238, 267
FspBI CTAG 1 cut(s) 32
GlaI GCGC 1 cut(s) 1065
GluI GCNGC 2 cut(s) 238, 267
GsaI CCCAGC 1 cut(s) 325
GsuI CTGGAG 1 cut(s) 383
HaeIII GGCC 1 cut(s) 1078
HapII CCGG 9 cut(s) 102, 126, 326, 338, 478, 495, 554, 659, 764
HgaI GACGC 2 cut(s) 188, 938
HhaI GCGC 1 cut(s) 1066
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 3 cut(s) 313, 955, 1112
Hin6I GCGC 1 cut(s) 1064
HinP1I GCGC 1 cut(s) 1064
HincII GTYRAC 3 cut(s) 283, 433, 505
HindII GTYRAC 3 cut(s) 283, 433, 505
HindIII AAGCTT 1 cut(s) 1181
HinfI GANTC 1 cut(s) 287
HpaII CCGG 9 cut(s) 102, 126, 326, 338, 478, 495, 554, 659, 764
HphI GGTGA 6 cut(s) 91, 151, 401, 451, 532, 994
Hpy166II GTNNAC 7 cut(s) 283, 348, 409, 433, 505, 736, 1089
Hpy188I TCNGA 5 cut(s) 178, 393, 894, 1017, 1054
Hpy188III TCNNGA 2 cut(s) 227, 600
Hpy8I GTNNAC 7 cut(s) 283, 348, 409, 433, 505, 736, 1089
Hpy99I CGWCG 2 cut(s) 506, 509
HpyAV CCTTC 6 cut(s) 72, 742, 787, 835, 850, 1119
HpyCH4III ACNGT 5 cut(s) 280, 382, 550, 616, 1324
HpyCH4IV ACGT 3 cut(s) 501, 715, 1100
HpyF10VI GCNNNNNNNGC 1 cut(s) 1034
HpyF3I CTNAG 3 cut(s) 19, 175, 809
HpySE526I ACGT 3 cut(s) 501, 715, 1100
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 3 cut(s) 313, 955, 1112
HspAI GCGC 1 cut(s) 1064
KpnI GGTACC 1 cut(s) 554
KroI GCCGGC 1 cut(s) 325
KroNI GCCGGC 1 cut(s) 327
Ksp22I TGATCA 2 cut(s) 312, 388
Kzo9I GATC 5 cut(s) 229, 312, 388, 871, 948
LmnI GCTCC 2 cut(s) 340, 865
LweI GCATC 5 cut(s) 547, 837, 1015, 1047, 1234
MaeI CTAG 1 cut(s) 32
MaeII ACGT 3 cut(s) 501, 715, 1100
MaeIII GTNAC 5 cut(s) 457, 480, 667, 877, 982
MalI GATC 5 cut(s) 231, 314, 390, 873, 950
MbiI CCGCTC 1 cut(s) 343
MboI GATC 5 cut(s) 229, 312, 388, 871, 948
MboII GAAGA 5 cut(s) 57, 184, 224, 292, 946
MhlI GDGCHC 3 cut(s) 501, 1091, 1146
MluCI AATT 4 cut(s) 121, 196, 709, 1148
MlyI GAGTC 1 cut(s) 281
MmeI TCCRAC 2 cut(s) 723, 995
Mph1103I ATGCAT 1 cut(s) 1030
MroNI GCCGGC 1 cut(s) 325
MseI TTAA 3 cut(s) 759, 1326, 1335
MspI CCGG 9 cut(s) 102, 126, 326, 338, 478, 495, 554, 659, 764
MspR9I CCNGG 1 cut(s) 496
Mva1269I GAATGC 1 cut(s) 329
MvnI CGCG 1 cut(s) 932
MwoI GCNNNNNNNGC 1 cut(s) 1034
NaeI GCCGGC 1 cut(s) 327
NciI CCSGG 1 cut(s) 496
NdeII GATC 5 cut(s) 229, 312, 388, 871, 948
NgoMIV GCCGGC 1 cut(s) 325
NlaIII CATG 3 cut(s) 313, 955, 1112
NlaIV GGNNCC 2 cut(s) 552, 867
NmuCI GTSAC 3 cut(s) 457, 480, 982
NsiI ATGCAT 1 cut(s) 1030
PaqCI CACCTGC 1 cut(s) 508
PctI GAATGC 1 cut(s) 329
PdiI GCCGGC 1 cut(s) 327
PkrI GCNGC 2 cut(s) 239, 268
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
Ppu21I YACGTR 1 cut(s) 716
PsiI TTATAA 1 cut(s) 570
PspEI GGTNACC 1 cut(s) 457
PspFI CCCAGC 1 cut(s) 321
PspN4I GGNNCC 2 cut(s) 552, 867
PspPI GGNCC 1 cut(s) 345
RsaI GTAC 4 cut(s) 384, 552, 613, 1204
RsaNI GTAC 4 cut(s) 383, 551, 612, 1203
SalI GTCGAC 1 cut(s) 503
SaqAI TTAA 3 cut(s) 759, 1326, 1335
SatI GCNGC 2 cut(s) 238, 267
Sau3AI GATC 5 cut(s) 229, 312, 388, 871, 948
Sau96I GGNCC 1 cut(s) 345
ScaI AGTACT 1 cut(s) 613
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 1 cut(s) 496
SduI GDGCHC 3 cut(s) 501, 1091, 1146
SfaNI GCATC 5 cut(s) 547, 837, 1015, 1047, 1234
SfcI CTRYAG 1 cut(s) 1320
SgrDI CGTCGACG 1 cut(s) 503
SinI GGWCC 1 cut(s) 345
Sse9I AATT 4 cut(s) 121, 196, 709, 1148
SsiI CCGC 3 cut(s) 238, 267, 343
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 494
TaaI ACNGT 5 cut(s) 280, 382, 550, 616, 1324
TaiI ACGT 3 cut(s) 504, 718, 1103
TaqI TCGA 5 cut(s) 504, 599, 727, 908, 1146
TasI AATT 4 cut(s) 121, 196, 709, 1148
TatI WGTACW 1 cut(s) 611
TauI GCSGC 2 cut(s) 240, 269
Tru1I TTAA 3 cut(s) 759, 1326, 1335
Tru9I TTAA 3 cut(s) 759, 1326, 1335
TscAI CASTG 4 cut(s) 385, 519, 1044, 1195
TseFI GTSAC 3 cut(s) 457, 480, 982
Tsp45I GTSAC 3 cut(s) 457, 480, 982
TspGWI ACGGA 1 cut(s) 14
TspRI CASTG 4 cut(s) 385, 519, 1044, 1195
VneI GTGCAC 1 cut(s) 1087
VpaK11BI GGWCC 1 cut(s) 345
XapI RAATTY 2 cut(s) 121, 709
XmiI GTMKAC 1 cut(s) 504
XspI CTAG 1 cut(s) 32
ZrmI AGTACT 1 cut(s) 613
Zsp2I ATGCAT 1 cut(s) 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.