Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0015461

Physical Location & Seq

Genomic Coordinates Forward (+)

9858445 .. 9861389

Gene Structure 2945 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1120600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1467 bp

ATGGGGAAAATCCCACCATCTCTGCGCTCATCAGTTTCAACCTCCATCATCAAAAAGCCACAATCTTTAATCCCAAACGAATCCCCATCTCGCAAGCCCCATAACTTTCCCAAGAAACCCACCAGGAAAATCCCACCCCAACTTTCCGAGAAAACCCAGGAACCCACCTCCTTCCGCAACCCCTTCACCTCCCCAAACATCTCCGACGCCAAGAGTGTCTTCACCTCCATCGTCGCCGCCACGAAATCCCCACTCGACCTCCGCGTCCACAACGCCCTGCTCCAATCCTACGCCGCAATCTCCACCGTCAACGACTCCATATCTCTCTTCAACCACATGATCAAAACACACCCTGCGTTCTCCCCCGACAAATCGACCTACCATGTTTTGCTCTCCCAGTCCTGCAAAGCAGGGGATGAGACACTGGGGTCCGTACACCAGGTACTCAACTTTATGCTCACCAATGGCTTCAGTCCCGATAAGGTCACCACAGATATCGCTGTCCGGTCACTCTGTTCTGCCGGTAATGTTGACCTCGCTGTTGAATTGGTTAAAGAAATGGCGATGAAAAACGCAGCCCCGGACAGTTATACATACAATTTTCTGGTGAAGTGTCTCTGCAAGTCGAGATCTGTGAGTAGTGTTTATGAGTTTATTGAAAGTATGAAAAGTTCTTTGAATTTAAAGCCTGATCTTGTCACTTATACTATTTTGATTGACAATGTGACGAATAGAGAGAATTTACGCGAGGCGACTAAGTTAGTGGAAATGTTAGCTGAGGAGGGATTTAAGCCTGATTGCTATATTTACAATACAATTATGAAAGGTTTTTGTAAATTGAATTTGGGCAAAGAGGCAGTTGATGTTTTCAAGAAAATGAAGGAGGAGGGCGTGGAGCCTGATCTTGTAACGTATAATACTTTGATTTTTGGGTTATCGAAATGTGGAAGGATTATGGAGGCTAAGAAGTATCTGGGTGTCATGGTGGAAATGGGACATTTCCCGGATGCGGTGACATACACTTCATTGATGAATGGAATGTGTCGGAAGGGGGATGTCTTGGGTGCAATGGAGTTGTTGGAGGAGATGCAAGCACAGGGGTGCCAACCAAATGTTTGCACGTATAATACGTTGCTTCATGGATTATGCAAGTCAAAACTTTTAGAGAAAGGGTTAGAATTGTATGGGCTGATGAAAGAATGTGGGATGAAGCTTGATACAGCTTCTTATGCTACACTTGTGAGGGCACTTTGTAGGGAGGGTAGGGTTGCAGATGCTTACGAAGTGTTTGATTATGCAGTTGAGAGTAAGAGTGTAACTGATGTTGCTGCTTACACGACTTTGGAGGGTACACTGAAGTGGCTTAAGAAAGCAAGGGAGCAAGGTCTTGCTTTTCGTTACAGGACTGTTGGAGGTTGTTTGAGTTGTCAACAATGGTCCCTGGTGAAGCAACAAGACTGCATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

489

Amino Acids

54.19

Weight (kDa)

8.61

Isoelectric Point (pI)

32.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	108 - 225	1.4e-07	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	155 - 187	1e-05	PPR repeat
PPR_2	PF13041	159 - 208	9.5e-11	PPR repeat family
TPR_24	PF23276	174 - 293	4e-06	Fungal tetratrico peptide repeats
PPR_2	PF13041	198 - 241	2.2e-08	PPR repeat family
PPR_3	PF13812	226 - 277	8.1e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	230 - 279	1.9e-09	PPR repeat family
PPR_3	PF13812	260 - 309	6.7e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	262 - 294	5.6e-09	PPR repeat
PPR_2	PF13041	265 - 314	2.1e-16	PPR repeat family
PPR	PF01535	269 - 298	3.3e-08	PPR repeat
PPR_long	PF17177	285 - 438	6.7e-14	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	286 - 396	4e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	297 - 328	3e-09	PPR repeat
PPR_2	PF13041	300 - 349	2.6e-14	PPR repeat family
PPR	PF01535	303 - 332	5e-06	PPR repeat
PPR_3	PF13812	330 - 380	6.2e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	331 - 364	2.1e-12	PPR repeat
PPR_2	PF13041	335 - 384	1.2e-19	PPR repeat family
PPR	PF01535	338 - 368	2.4e-08	PPR repeat
PPR_1	PF12854	367 - 398	1.9e-09	PPR repeat
PPR_2	PF13041	370 - 419	1.1e-13	PPR repeat family
PPR	PF01535	374 - 402	2.6e-07	PPR repeat
PPR_1	PF12854	402 - 432	2.2e-08	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17670 AT2G17670
fragaria_vesca	FvH4_1g10671
malus_domestica	MD02G1120600.v1.1
prunus_persica	Prupe.7G178400_v2.0.a1
rosa_laevigata	RLG00000016739
rosa_multiflora	Rmu_co8429607.1_g000001 Rmu_sc0000974.1_g000027 Rmu_sc0008442.1_g000010
rosa_roxburghii	Rroxscaffold_6G00394240
rosa_rugosa	Rorug02G0068500
rosa_samantha	Rh2AG115200 Rh2BG118100 Rh2CG119700 Rh2DG119400
rosa_wichuraiana	Rw2G009000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	263, 427
AccB1I	GGYRCC	1 cut(s)	1101
AccII	CGCG	2 cut(s)	264, 747
AciI	CCGC	5 cut(s)	175, 237, 262, 294, 1010
AcsI	RAATTY	3 cut(s)	679, 739, 841
AcuI	CTGAAG	2 cut(s)	454, 1376
AcyI	GRCGYC	1 cut(s)	207
AfaI	GTAC	3 cut(s)	435, 444, 1351
AfiI	CCNNNNNNNGG	1 cut(s)	1009
AflII	CTTAAG	1 cut(s)	1364
AgsI	TTSAA	7 cut(s)	39, 331, 545, 659, 679, 841, 871
AjnI	CCWGG	4 cut(s)	122, 156, 438, 1440
AjuI	GAANNNNNNNTTGG	2 cut(s)	203, 235
AleI	CACNNNNGTG	2 cut(s)	1099, 1357
AluBI	AGCT	3 cut(s)	776, 1213, 1223
AluI	AGCT	3 cut(s)	776, 1213, 1223
Alw26I	GTCTC	2 cut(s)	413, 620
ApeKI	GCWGC	2 cut(s)	575, 1328
ApoI	RAATTY	3 cut(s)	679, 739, 841
AspLEI	GCGC	1 cut(s)	27
AspS9I	GGNCC	2 cut(s)	429, 1437
AsuC2I	CCSGG	2 cut(s)	581, 1004
AsuHPI	GGTGA	7 cut(s)	178, 214, 451, 478, 619, 1024, 1456
AvaII	GGWCC	2 cut(s)	429, 1437
BaeGI	GKGCMC	1 cut(s)	1249
BanI	GGYRCC	1 cut(s)	1101
BbsI	GAAGAC	1 cut(s)	211
BbvCI	CCTCAGC	1 cut(s)	777
BbvI	GCAGC	2 cut(s)	587, 1315
BccI	CCATC	4 cut(s)	25, 53, 94, 236
BciT130I	CCWGG	4 cut(s)	124, 158, 440, 1442
BclI	TGATCA	1 cut(s)	339
BcnI	CCSGG	2 cut(s)	581, 1004
BcoDI	GTCTC	2 cut(s)	413, 620
BfrI	CTTAAG	1 cut(s)	1364
BglII	AGATCT	1 cut(s)	629
BisI	GCNGC	4 cut(s)	237, 294, 576, 1329
BlsI	GCNGC	4 cut(s)	238, 295, 577, 1330
Bme1390I	CCNGG	6 cut(s)	124, 158, 440, 581, 1004, 1442
Bme18I	GGWCC	2 cut(s)	429, 1437
BmgT120I	GGNCC	2 cut(s)	429, 1437
BmiI	GGNNCC	5 cut(s)	162, 430, 897, 1103, 1439
BmrFI	CCNGG	6 cut(s)	124, 158, 440, 581, 1004, 1442
BmrI	ACTGGG	2 cut(s)	391, 434
BmsI	GCATC	3 cut(s)	997, 1077, 1264
BmuI	ACTGGG	2 cut(s)	391, 434
BpiI	GAAGAC	1 cut(s)	211
Bpu10I	CCTNAGC	1 cut(s)	777
BpuMI	CCSGG	2 cut(s)	581, 1004
BsaAI	YACGTR	1 cut(s)	1122
BsaHI	GRCGYC	1 cut(s)	207
BsaJI	CCNNGG	3 cut(s)	156, 579, 1440
BsaWI	WCCGGW	1 cut(s)	504
BsaXI	ACNNNNNCTCC	2 cut(s)	243, 273
Bsc4I	CCNNNNNNNGG	1 cut(s)	1009
Bse118I	RCCGGY	1 cut(s)	521
Bse1I	ACTGG	2 cut(s)	397, 429
Bse3DI	GCAATG	1 cut(s)	1074
BseBI	CCWGG	4 cut(s)	124, 158, 440, 1442
BseDI	CCNNGG	3 cut(s)	156, 579, 1440
BseGI	GGATG	4 cut(s)	421, 1012, 1060, 1212
BseLI	CCNNNNNNNGG	1 cut(s)	1009
BseMI	GCAATG	1 cut(s)	1074
BseMII	CTCAG	1 cut(s)	768
BseNI	ACTGG	2 cut(s)	397, 429
BseRI	GAGGAG	3 cut(s)	794, 899, 1097
BseSI	GKGCMC	1 cut(s)	1249
BseXI	GCAGC	2 cut(s)	587, 1315
Bsh1236I	CGCG	2 cut(s)	264, 747
BshNI	GGYRCC	1 cut(s)	1101
BsiSI	CCGG	4 cut(s)	505, 522, 581, 1004
BslFI	GGGAC	3 cut(s)	459, 1008, 1423
BslI	CCNNNNNNNGG	1 cut(s)	1009
BsmAI	GTCTC	2 cut(s)	413, 620
BsmFI	GGGAC	3 cut(s)	459, 1008, 1423
Bsp1286I	GDGCHC	1 cut(s)	1249
Bsp143I	GATC	4 cut(s)	339, 629, 691, 901
BspACI	CCGC	5 cut(s)	175, 237, 262, 294, 1010
BspCNI	CTCAG	1 cut(s)	769
BspFNI	CGCG	2 cut(s)	264, 747
BspLI	GGNNCC	5 cut(s)	162, 430, 897, 1103, 1439
BspT107I	GGYRCC	1 cut(s)	1101
BspTI	CTTAAG	1 cut(s)	1364
BsrDI	GCAATG	1 cut(s)	1074
BsrFI	RCCGGY	1 cut(s)	521
BsrI	ACTGG	2 cut(s)	397, 429
BssAI	RCCGGY	1 cut(s)	521
BssECI	CCNNGG	3 cut(s)	156, 579, 1440
BssMI	GATC	4 cut(s)	339, 629, 691, 901
BssNI	GRCGYC	1 cut(s)	207
Bst2UI	CCWGG	4 cut(s)	124, 158, 440, 1442
Bst4CI	ACNGT	3 cut(s)	307, 587, 1408
Bst6I	CTCTTC	1 cut(s)	332
BstACI	GRCGYC	1 cut(s)	207
BstAFI	CTTAAG	1 cut(s)	1364
BstBAI	YACGTR	1 cut(s)	1122
BstC8I	GCNNGC	2 cut(s)	95, 1092
BstDEI	CTNAG	3 cut(s)	756, 777, 963
BstEII	GGTNACC	1 cut(s)	484
BstF5I	GGATG	4 cut(s)	421, 1012, 1060, 1212
BstFNI	CGCG	2 cut(s)	264, 747
BstHHI	GCGC	1 cut(s)	27
BstKTI	GATC	4 cut(s)	342, 632, 694, 904
BstMAI	GTCTC	2 cut(s)	413, 620
BstMBI	GATC	4 cut(s)	339, 629, 691, 901
BstMWI	GCNNNNNNNGC	1 cut(s)	1229
BstNI	CCWGG	4 cut(s)	124, 158, 440, 1442
BstNSI	RCATGY	1 cut(s)	1465
BstPI	GGTNACC	1 cut(s)	484
BstSCI	CCNGG	6 cut(s)	122, 156, 438, 579, 1002, 1440
BstSLI	GKGCMC	1 cut(s)	1249
BstUI	CGCG	2 cut(s)	264, 747
BstV1I	GCAGC	2 cut(s)	587, 1315
BstV2I	GAAGAC	1 cut(s)	211
BstX2I	RGATCY	1 cut(s)	629
BstYI	RGATCY	1 cut(s)	629
BtgZI	GCGATG	1 cut(s)	578
BtsCI	GGATG	4 cut(s)	421, 1012, 1060, 1212
BtsIMutI	CAGTG	2 cut(s)	422, 1352
Cac8I	GCNNGC	2 cut(s)	95, 1092
CfoI	GCGC	1 cut(s)	27
Cfr10I	RCCGGY	1 cut(s)	521
Cfr13I	GGNCC	2 cut(s)	429, 1437
CseI	GACGC	2 cut(s)	215, 253
CsiI	ACCWGGT	1 cut(s)	438
Csp6I	GTAC	3 cut(s)	434, 443, 1350
CviAII	CATG	5 cut(s)	337, 383, 982, 1139, 1462
CviQI	GTAC	3 cut(s)	434, 443, 1350
DdeI	CTNAG	3 cut(s)	756, 777, 963
DpnI	GATC	4 cut(s)	341, 631, 693, 903
DpnII	GATC	4 cut(s)	339, 629, 691, 901
DraI	TTTAAA	1 cut(s)	684
DrdI	GACNNNNNNGTC	2 cut(s)	263, 427
DseDI	GACNNNNNNGTC	2 cut(s)	263, 427
Eam1104I	CTCTTC	1 cut(s)	332
EarI	CTCTTC	1 cut(s)	332
Eco32I	GATATC	1 cut(s)	496
Eco47I	GGWCC	2 cut(s)	429, 1437
Eco57I	CTGAAG	2 cut(s)	454, 1376
Eco91I	GGTNACC	1 cut(s)	484
EcoO65I	GGTNACC	1 cut(s)	484
EcoRII	CCWGG	4 cut(s)	122, 156, 438, 1440
EcoRV	GATATC	1 cut(s)	496
FaeI	CATG	5 cut(s)	340, 386, 985, 1142, 1465
FalI	AAGNNNNNCTT	2 cut(s)	203, 235
FaqI	GGGAC	3 cut(s)	459, 1008, 1423
FatI	CATG	5 cut(s)	336, 382, 981, 1138, 1461
FbaI	TGATCA	1 cut(s)	339
Fnu4HI	GCNGC	4 cut(s)	237, 294, 576, 1329
FokI	GGATG	4 cut(s)	428, 1019, 1067, 1219
Fsp4HI	GCNGC	4 cut(s)	237, 294, 576, 1329
GlaI	GCGC	1 cut(s)	26
GluI	GCNGC	4 cut(s)	237, 294, 576, 1329
HapII	CCGG	4 cut(s)	505, 522, 581, 1004
HgaI	GACGC	2 cut(s)	215, 253
HhaI	GCGC	1 cut(s)	27
Hin1I	GRCGYC	1 cut(s)	207
Hin1II	CATG	5 cut(s)	340, 386, 985, 1142, 1465
Hin6I	GCGC	1 cut(s)	25
HinP1I	GCGC	1 cut(s)	25
HincII	GTYRAC	3 cut(s)	310, 532, 1430
HindII	GTYRAC	3 cut(s)	310, 532, 1430
HindIII	AAGCTT	1 cut(s)	1211
HinfI	GANTC	2 cut(s)	80, 314
HpaII	CCGG	4 cut(s)	505, 522, 581, 1004
HphI	GGTGA	7 cut(s)	178, 214, 451, 478, 619, 1024, 1456
Hpy166II	GTNNAC	6 cut(s)	268, 310, 436, 532, 1352, 1430
Hpy188I	TCNGA	3 cut(s)	148, 205, 1047
Hpy188III	TCNNGA	3 cut(s)	476, 627, 871
Hpy8I	GTNNAC	6 cut(s)	268, 310, 436, 532, 1352, 1430
Hpy99I	CGWCG	2 cut(s)	209, 236
HpyAV	CCTTC	5 cut(s)	181, 193, 874, 942, 1042
HpyCH4III	ACNGT	3 cut(s)	307, 587, 1408
HpyCH4IV	ACGT	3 cut(s)	911, 1121, 1130
HpyCH4V	TGCA	9 cut(s)	405, 621, 1067, 1090, 1119, 1149, 1271, 1298, 1461
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1229
HpyF3I	CTNAG	3 cut(s)	756, 777, 963
HpySE526I	ACGT	3 cut(s)	911, 1121, 1130
Hsp92I	GRCGYC	1 cut(s)	207
Hsp92II	CATG	5 cut(s)	340, 386, 985, 1142, 1465
HspAI	GCGC	1 cut(s)	25
Ksp22I	TGATCA	1 cut(s)	339
Kzo9I	GATC	4 cut(s)	339, 629, 691, 901
LmnI	GCTCC	3 cut(s)	285, 895, 1378
Lsp1109I	GCAGC	2 cut(s)	587, 1315
LweI	GCATC	3 cut(s)	997, 1077, 1264
MabI	ACCWGGT	1 cut(s)	438
MaeII	ACGT	3 cut(s)	911, 1121, 1130
MaeIII	GTNAC	8 cut(s)	484, 507, 697, 724, 907, 1012, 1315, 1397
MalI	GATC	4 cut(s)	341, 631, 693, 903
MboI	GATC	4 cut(s)	339, 629, 691, 901
MboII	GAAGA	2 cut(s)	211, 319
MflI	RGATCY	1 cut(s)	629
MhlI	GDGCHC	1 cut(s)	1249
MluCI	AATT	8 cut(s)	545, 598, 679, 739, 816, 836, 841, 1178
MlyI	GAGTC	1 cut(s)	308
MmeI	TCCRAC	4 cut(s)	228, 1025, 1059, 1390
MseI	TTAA	5 cut(s)	68, 552, 683, 789, 1365
MslI	CAYNNNNRTG	2 cut(s)	1099, 1357
MspCI	CTTAAG	1 cut(s)	1364
MspI	CCGG	4 cut(s)	505, 522, 581, 1004
MspR9I	CCNGG	6 cut(s)	124, 158, 440, 581, 1004, 1442
MvaI	CCWGG	4 cut(s)	124, 158, 440, 1442
MvnI	CGCG	2 cut(s)	264, 747
MwoI	GCNNNNNNNGC	1 cut(s)	1229
NciI	CCSGG	2 cut(s)	581, 1004
NdeII	GATC	4 cut(s)	339, 629, 691, 901
NlaIII	CATG	5 cut(s)	340, 386, 985, 1142, 1465
NlaIV	GGNNCC	5 cut(s)	162, 430, 897, 1103, 1439
NmuCI	GTSAC	5 cut(s)	484, 507, 697, 724, 1012
NspI	RCATGY	1 cut(s)	1465
OliI	CACNNNNGTG	2 cut(s)	1099, 1357
PcsI	WCGNNNNNNNCGW	2 cut(s)	261, 1127
PfeI	GAWTC	1 cut(s)	80
PfoI	TCCNGGA	1 cut(s)	1002
PkrI	GCNGC	4 cut(s)	238, 295, 577, 1330
PleI	GAGTC	1 cut(s)	308
PpsI	GAGTC	1 cut(s)	308
Ppu21I	YACGTR	1 cut(s)	1122
Psp6I	CCWGG	4 cut(s)	122, 156, 438, 1440
PspEI	GGTNACC	1 cut(s)	484
PspGI	CCWGG	4 cut(s)	122, 156, 438, 1440
PspN4I	GGNNCC	5 cut(s)	162, 430, 897, 1103, 1439
PspPI	GGNCC	2 cut(s)	429, 1437
PsrI	GAACNNNNNNTAC	2 cut(s)	655, 687
PsuI	RGATCY	1 cut(s)	629
RsaI	GTAC	3 cut(s)	435, 444, 1351
RsaNI	GTAC	3 cut(s)	434, 443, 1350
RseI	CAYNNNNRTG	2 cut(s)	1099, 1357
SaqAI	TTAA	5 cut(s)	68, 552, 683, 789, 1365
SatI	GCNGC	4 cut(s)	237, 294, 576, 1329
Sau3AI	GATC	4 cut(s)	339, 629, 691, 901
Sau96I	GGNCC	2 cut(s)	429, 1437
SchI	GAGTC	1 cut(s)	308
ScrFI	CCNGG	6 cut(s)	124, 158, 440, 581, 1004, 1442
SduI	GDGCHC	1 cut(s)	1249
SexAI	ACCWGGT	1 cut(s)	438
SfaNI	GCATC	3 cut(s)	997, 1077, 1264
SinI	GGWCC	2 cut(s)	429, 1437
SmiMI	CAYNNNNRTG	2 cut(s)	1099, 1357
SmlI	CTYRAG	1 cut(s)	1364
SmoI	CTYRAG	1 cut(s)	1364
Sse9I	AATT	8 cut(s)	545, 598, 679, 739, 816, 836, 841, 1178
SsiI	CCGC	5 cut(s)	175, 237, 262, 294, 1010
StyD4I	CCNGG	6 cut(s)	122, 156, 438, 579, 1002, 1440
TaaI	ACNGT	3 cut(s)	307, 587, 1408
TaiI	ACGT	3 cut(s)	914, 1124, 1133
TaqI	TCGA	4 cut(s)	255, 374, 626, 938
TasI	AATT	8 cut(s)	545, 598, 679, 739, 816, 836, 841, 1178
TauI	GCSGC	2 cut(s)	239, 296
TfiI	GAWTC	1 cut(s)	80
Tru1I	TTAA	5 cut(s)	68, 552, 683, 789, 1365
Tru9I	TTAA	5 cut(s)	68, 552, 683, 789, 1365
TscAI	CASTG	2 cut(s)	429, 1359
TseFI	GTSAC	5 cut(s)	484, 507, 697, 724, 1012
TseI	GCWGC	2 cut(s)	575, 1328
Tsp45I	GTSAC	5 cut(s)	484, 507, 697, 724, 1012
TspDTI	ATGAA	9 cut(s)	581, 680, 836, 893, 1014, 1046, 1127, 1208, 1223
TspGWI	ACGGA	1 cut(s)	421
TspRI	CASTG	2 cut(s)	429, 1359
Vha464I	CTTAAG	1 cut(s)	1364
VpaK11BI	GGWCC	2 cut(s)	429, 1437
XapI	RAATTY	3 cut(s)	679, 739, 841
XceI	RCATGY	1 cut(s)	1465

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.