Rorug02G0068500

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0015461
Physical Location & Seq
Forward (+)
5335719 .. 5337336
1618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0068500.1

Sequence Viewer

Length: 297 bp
ATGAACTTAGTCGACTCGCCAGACTGTAATCCGCAGCCACAAGAAGAAGACCCCTTCCTCAAATTCATTAATTACGCCAGGTCTGCTCTCTCGCCTGAAACAGACGAAGATTCAGATCCGAAAGGAAATGGGGCGGAGACCGTCCGACCCAGTTGGAATTGGATCTCCTCTCGGATTGTCAAGACCTGCAGCGCCTACTCCAGCGGCGATTTTGCTCTCCGATCTCTCACAGATATGATTGGAATTTTTGTTGATTTGATTACGCATGGCATGAGCAAAATAAGGACAGGCCTGTGA

Protein Analysis

98

Amino Acids

10.85

Weight (kDa)

4.61

Isoelectric Point (pI)

54.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC24_OB1 PF17246 19 - 69 8.5e-13 Cell division control protein 24, OB domain 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015461)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17670 AT2G17670
fragaria_vesca FvH4_1g10671
malus_domestica MD02G1120600.v1.1
prunus_persica Prupe.7G178400_v2.0.a1
rosa_laevigata RLG00000016739
rosa_multiflora Rmu_co8429607.1_g000001 Rmu_sc0000974.1_g000027 Rmu_sc0008442.1_g000010
rosa_roxburghii Rroxscaffold_6G00394240
rosa_rugosa Rorug02G0068500
rosa_samantha Rh2AG115200 Rh2BG118100 Rh2CG119700 Rh2DG119400
rosa_wichuraiana Rw2G009000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 194
AccI GTMKAC 1 cut(s) 12
AciI CCGC 3 cut(s) 32, 134, 204
AclWI GGATC 2 cut(s) 110, 170
AcsI RAATTY 2 cut(s) 62, 243
AjnI CCWGG 1 cut(s) 77
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 2 cut(s) 110, 170
AoxI GGCC 1 cut(s) 289
ApeKI GCWGC 2 cut(s) 34, 189
ApoI RAATTY 2 cut(s) 62, 243
AseI ATTAAT 1 cut(s) 69
AspLEI GCGC 1 cut(s) 194
BbsI GAAGAC 1 cut(s) 54
BbvI GCAGC 2 cut(s) 46, 201
BciT130I CCWGG 1 cut(s) 79
BcoDI GTCTC 1 cut(s) 131
BfmI CTRYAG 1 cut(s) 187
BfoI RGCGCY 1 cut(s) 195
BfuAI ACCTGC 1 cut(s) 194
BisI GCNGC 3 cut(s) 35, 190, 205
BlsI GCNGC 3 cut(s) 36, 191, 206
Bme1390I CCNGG 1 cut(s) 79
BmrFI CCNGG 1 cut(s) 79
BmrI ACTGGG 1 cut(s) 144
BmuI ACTGGG 1 cut(s) 144
BpiI GAAGAC 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 184
BsaBI GATNNNNATC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 131
Bse1I ACTGG 1 cut(s) 150
Bse8I GATNNNNATC 1 cut(s) 114
BseBI CCWGG 1 cut(s) 79
BseJI GATNNNNATC 1 cut(s) 114
BseNI ACTGG 1 cut(s) 150
BseRI GAGGAG 1 cut(s) 157
BseXI GCAGC 2 cut(s) 46, 201
BshFI GGCC 1 cut(s) 291
BsmAI GTCTC 1 cut(s) 131
BsnI GGCC 1 cut(s) 291
Bso31I GGTCTC 1 cut(s) 131
Bsp143I GATC 3 cut(s) 115, 162, 221
BspACI CCGC 3 cut(s) 32, 134, 204
BspANI GGCC 1 cut(s) 291
BspMAI CTGCAG 1 cut(s) 191
BspMI ACCTGC 1 cut(s) 194
BspPI GGATC 2 cut(s) 110, 170
BspTNI GGTCTC 1 cut(s) 131
BsrI ACTGG 1 cut(s) 150
BssMI GATC 3 cut(s) 115, 162, 221
Bst2UI CCWGG 1 cut(s) 79
Bst4CI ACNGT 2 cut(s) 26, 142
BstDEI CTNAG 1 cut(s) 7
BstH2I RGCGCY 1 cut(s) 195
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 3 cut(s) 118, 165, 224
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 3 cut(s) 115, 162, 221
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNI CCWGG 1 cut(s) 79
BstSCI CCNGG 1 cut(s) 77
BstSFI CTRYAG 1 cut(s) 187
BstV1I GCAGC 2 cut(s) 46, 201
BstV2I GAAGAC 1 cut(s) 54
BstX2I RGATCY 2 cut(s) 115, 162
BstYI RGATCY 2 cut(s) 115, 162
BsuRI GGCC 1 cut(s) 291
BveI ACCTGC 1 cut(s) 194
CfoI GCGC 1 cut(s) 194
CviAII CATG 2 cut(s) 266, 271
CviJI RGCY 2 cut(s) 37, 291
CviKI_1 RGCY 2 cut(s) 37, 291
DdeI CTNAG 1 cut(s) 7
DpnI GATC 3 cut(s) 117, 164, 223
DpnII GATC 3 cut(s) 115, 162, 221
EciI GGCGGA 1 cut(s) 149
Eco147I AGGCCT 1 cut(s) 291
Eco31I GGTCTC 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 77
FaeI CATG 2 cut(s) 269, 274
FaiI YATR 3 cut(s) 236, 267, 272
FatI CATG 2 cut(s) 265, 270
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 3 cut(s) 35, 190, 205
Fsp4HI GCNGC 3 cut(s) 35, 190, 205
GlaI GCGC 1 cut(s) 193
GluI GCNGC 3 cut(s) 35, 190, 205
GsuI CTGGAG 1 cut(s) 184
HaeII RGCGCY 1 cut(s) 195
HaeIII GGCC 1 cut(s) 291
HhaI GCGC 1 cut(s) 194
Hin1II CATG 2 cut(s) 269, 274
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 2 cut(s) 14, 110
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 5 cut(s) 115, 120, 146, 174, 221
Hpy188III TCNNGA 1 cut(s) 181
Hpy8I GTNNAC 1 cut(s) 13
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 2 cut(s) 26, 142
HpyCH4V TGCA 1 cut(s) 189
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 1 cut(s) 7
Hsp92II CATG 2 cut(s) 269, 274
HspAI GCGC 1 cut(s) 192
Kzo9I GATC 3 cut(s) 115, 162, 221
LpnPI CCDG 8 cut(s) 33, 64, 91, 108, 163, 199, 214, 273
Lsp1109I GCAGC 2 cut(s) 46, 201
MalI GATC 3 cut(s) 117, 164, 223
MboI GATC 3 cut(s) 115, 162, 221
MboII GAAGA 3 cut(s) 56, 59, 119
MflI RGATCY 2 cut(s) 115, 162
MluCI AATT 4 cut(s) 62, 70, 157, 243
MlyI GAGTC 1 cut(s) 8
MmeI TCCRAC 2 cut(s) 134, 169
MnlI CCTC 2 cut(s) 68, 178
MseI TTAA 1 cut(s) 69
MslI CAYNNNNRTG 1 cut(s) 233
MspA1I CMGCKG 1 cut(s) 204
MspR9I CCNGG 1 cut(s) 79
MvaI CCWGG 1 cut(s) 79
MwoI GCNNNNNNNGC 1 cut(s) 83
NdeII GATC 3 cut(s) 115, 162, 221
NlaIII CATG 2 cut(s) 269, 274
PceI AGGCCT 1 cut(s) 291
PfeI GAWTC 1 cut(s) 110
PkrI GCNGC 3 cut(s) 36, 191, 206
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
PshBI ATTAAT 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 77
PspGI CCWGG 1 cut(s) 77
PstI CTGCAG 1 cut(s) 191
PsuI RGATCY 2 cut(s) 115, 162
RseI CAYNNNNRTG 1 cut(s) 233
SalI GTCGAC 1 cut(s) 11
SaqAI TTAA 1 cut(s) 69
SatI GCNGC 3 cut(s) 35, 190, 205
Sau3AI GATC 3 cut(s) 115, 162, 221
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 1 cut(s) 79
SetI ASST 2 cut(s) 83, 188
SfcI CTRYAG 1 cut(s) 187
SmiMI CAYNNNNRTG 1 cut(s) 233
Sse9I AATT 4 cut(s) 62, 70, 157, 243
SseBI AGGCCT 1 cut(s) 291
SsiI CCGC 3 cut(s) 32, 134, 204
StuI AGGCCT 1 cut(s) 291
StyD4I CCNGG 1 cut(s) 77
TaaI ACNGT 2 cut(s) 26, 142
TaqI TCGA 1 cut(s) 12
TasI AATT 4 cut(s) 62, 70, 157, 243
TauI GCSGC 1 cut(s) 207
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TseI GCWGC 2 cut(s) 34, 189
TspDTI ATGAA 2 cut(s) 17, 55
VspI ATTAAT 1 cut(s) 69
XapI RAATTY 2 cut(s) 62, 243
XmiI GTMKAC 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.