FvH4_1g12770

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0016697
Physical Location & Seq
Forward (+)
7049066 .. 7050448
1383 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g12770.t1

Sequence Viewer

Length: 1092 bp
ATGGATGTAGAGCAAGTCTTTCACATGACTGGAGGCATTGGCCACAGAAGCTATGCACAAAACTCTTCTCTCCAAAAGAAGGCATCTGAAATGGTGAAGGACATGACCTTAGAAACAGTGCAAGAGCTTTACCTTACAACAGCTCCAAAGAGCTTAGGCATAGCTGATCTGGGTTGCTCATCAGGACCCAACACCTTGTCAATCATGAAAGAGATCATTGAAGTAGTCCAGGGAACAAGTAGAAAAGCCTTTCATGCAGCACCAGAATTCAGAGTGTACCTTAATGATCTTCCCACCAATGACTTCAACTCAATCTTCAAGTCCTTGCCAGAGTTCTCAAAGGACCTCAACAAAGAAATGAACAATGGGGGGTCTCCTTCCATTTACATAGGAGGTTATCCAGGCTCGTTTTATGGGAGGCTTTTTCCCAACAGCTCCTTGCACTTTGTTTATTCATCCTACAGTTTACACTGGCTGTCTAGGGTTCCTCCGGCTATATACTCAGAGGAAGGAAAATCCATAAACAAAGGCAGTGTTTACATTTCTGAATCAAGCCCTCCTGTAGTGGCTGAGGCATACTTGAAGCAATTCAAGGAAGACTTCACTTTGTTTCTTCAGTCAAGGTCTGAGGAGTTGCTTTCAGGAGGAAGAATGGTGCTGATCTTGTTGGGTAGGAGCGGCCCGGATCATGTGGATAGAGGCAACTCTTTCTTCTGGGAGCTTCTATATCAATCCCTTGCCATTTTGGTTTCCAAAGGAGAAGTTGAGAAGGAAAAGCTTGACTCATATGATGTGCATTTCTATGCTCCATCCAAAGATGAGTTAGAAGATGCAGTGAGGAAAGAGGGTTCGTTTCAATTGGACAGGTTGGAGATGTTTGAATTAGAGAGGGATCATCACAAGGACAATCAAAGCTATGCAACAAGTGTTGCAATGACTGTGAGGGCTATTCAAGAATCTATGATTGGCCAACATTTTGGAGAAGAAATCTTGGACAGTTTGTTTGAGAACTATAGAAGATTGGTAGGCGAAGAAATTGCCAAAGAAGATATTAAACCTACTACTTTTGGGATTGTACTTAGAAAACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

364

Amino Acids

40.95

Weight (kDa)

5.32

Isoelectric Point (pI)

48.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 38 - 362 5.5e-123 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36470
fragaria_vesca FvH4_1g12770
malus_domestica MD15G1256500.v1.1
prunus_persica Prupe.7G158400_v2.0.a1 Prupe.7G158400_v2.0.a1
pyrus_communis pycom02g11320 pycom15g22440
rosa_chinensis RchiOBHm_Chr2g0100891
rosa_laevigata RLG00000017021
rosa_multiflora Rmu_sc0001659.1_g000045
rosa_roxburghii Rroxscaffold_2G00141760
rosa_rugosa Rorug02G0093400
rosa_samantha Rh2BG146100
rosa_wichuraiana Rw2G011060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 678
AciI CCGC 1 cut(s) 678
AclWI GGATC 2 cut(s) 693, 900
AcoI YGGCCR 2 cut(s) 40, 967
AcsI RAATTY 1 cut(s) 266
AcuI CTGAAG 1 cut(s) 599
AfaI GTAC 2 cut(s) 278, 1077
AfiI CCNNNNNNNGG 1 cut(s) 79
AgsI TTSAA 8 cut(s) 221, 307, 319, 583, 592, 857, 881, 953
AjnI CCWGG 2 cut(s) 228, 400
AjuI GAANNNNNNNTTGG 2 cut(s) 948, 980
AloI GAACNNNNNNTCC 2 cut(s) 832, 864
AluBI AGCT 9 cut(s) 51, 127, 143, 153, 164, 435, 721, 778, 915
AluI AGCT 9 cut(s) 51, 127, 143, 153, 164, 435, 721, 778, 915
Alw26I GTCTC 1 cut(s) 378
AlwI GGATC 2 cut(s) 693, 900
AoxI GGCC 3 cut(s) 40, 679, 967
ApeKI GCWGC 1 cut(s) 257
ApoI RAATTY 1 cut(s) 266
ArsI GACNNNNNNTTYG 2 cut(s) 986, 1018
Asp700I GAANNNNTTC 1 cut(s) 587
AspS9I GGNCC 3 cut(s) 185, 343, 680
AsuC2I CCSGG 1 cut(s) 683
AsuHPI GGTGA 1 cut(s) 106
AvaII GGWCC 2 cut(s) 185, 343
BalI TGGCCA 2 cut(s) 42, 969
BbsI GAAGAC 1 cut(s) 603
BbvCI CCTCAGC 1 cut(s) 570
BbvI GCAGC 1 cut(s) 269
BccI CCATC 1 cut(s) 817
BcgI CGANNNNNNTGC 2 cut(s) 1019, 1053
BciT130I CCWGG 2 cut(s) 230, 402
BcnI CCSGG 1 cut(s) 683
BcoDI GTCTC 1 cut(s) 378
BfaI CTAG 1 cut(s) 480
BfmI CTRYAG 3 cut(s) 460, 561, 1012
BisI GCNGC 2 cut(s) 258, 679
BlsI GCNGC 2 cut(s) 259, 680
Bme1390I CCNGG 3 cut(s) 230, 402, 683
Bme18I GGWCC 2 cut(s) 185, 343
BmgT120I GGNCC 3 cut(s) 185, 343, 680
BmiI GGNNCC 2 cut(s) 187, 486
BmrFI CCNGG 3 cut(s) 230, 402, 683
BmsI GCATC 2 cut(s) 92, 820
BpiI GAAGAC 1 cut(s) 603
BpmI CTGGAG 1 cut(s) 51
Bpu10I CCTNAGC 2 cut(s) 154, 570
BpuMI CCSGG 1 cut(s) 683
BsaI GGTCTC 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 229
BsaXI ACNNNNNCTCC 2 cut(s) 127, 157
Bsc4I CCNNNNNNNGG 1 cut(s) 79
Bse1I ACTGG 2 cut(s) 34, 476
Bse3DI GCAATG 1 cut(s) 939
BseBI CCWGG 2 cut(s) 230, 402
BseDI CCNNGG 1 cut(s) 229
BseGI GGATG 3 cut(s) 10, 455, 809
BseLI CCNNNNNNNGG 1 cut(s) 79
BseMI GCAATG 1 cut(s) 939
BseMII CTCAG 3 cut(s) 516, 561, 618
BseNI ACTGG 2 cut(s) 34, 476
BseRI GAGGAG 1 cut(s) 644
BseXI GCAGC 1 cut(s) 269
BshFI GGCC 3 cut(s) 42, 681, 969
BsiSI CCGG 2 cut(s) 491, 683
BslI CCNNNNNNNGG 1 cut(s) 79
BsmAI GTCTC 1 cut(s) 378
BsnI GGCC 3 cut(s) 42, 681, 969
Bso31I GGTCTC 1 cut(s) 378
Bsp143I GATC 6 cut(s) 166, 213, 286, 660, 685, 892
BspACI CCGC 1 cut(s) 678
BspANI GGCC 3 cut(s) 42, 681, 969
BspCNI CTCAG 3 cut(s) 515, 562, 619
BspHI TCATGA 1 cut(s) 204
BspLI GGNNCC 2 cut(s) 187, 486
BspPI GGATC 2 cut(s) 693, 900
BspTNI GGTCTC 1 cut(s) 378
BsrBI CCGCTC 1 cut(s) 678
BsrDI GCAATG 1 cut(s) 939
BsrI ACTGG 2 cut(s) 34, 476
BssECI CCNNGG 1 cut(s) 229
BssMI GATC 6 cut(s) 166, 213, 286, 660, 685, 892
Bst2UI CCWGG 2 cut(s) 230, 402
Bst4CI ACNGT 4 cut(s) 118, 464, 940, 998
Bst6I CTCTTC 1 cut(s) 70
BstDEI CTNAG 6 cut(s) 109, 154, 502, 570, 627, 1079
BstF5I GGATG 3 cut(s) 10, 455, 809
BstKTI GATC 6 cut(s) 169, 216, 289, 663, 688, 895
BstMAI GTCTC 1 cut(s) 378
BstMBI GATC 6 cut(s) 166, 213, 286, 660, 685, 892
BstMWI GCNNNNNNNGC 2 cut(s) 48, 254
BstNI CCWGG 2 cut(s) 230, 402
BstSCI CCNGG 3 cut(s) 228, 400, 681
BstSFI CTRYAG 3 cut(s) 460, 561, 1012
BstV1I GCAGC 1 cut(s) 269
BstV2I GAAGAC 1 cut(s) 603
BstXI CCANNNNNNTGG 1 cut(s) 977
BsuRI GGCC 3 cut(s) 42, 681, 969
BtsCI GGATG 3 cut(s) 10, 455, 809
BtsI GCAGTG 2 cut(s) 538, 840
BtsIMutI CAGTG 4 cut(s) 123, 469, 538, 840
CciI TCATGA 1 cut(s) 204
Cfr13I GGNCC 3 cut(s) 185, 343, 680
Csp6I GTAC 2 cut(s) 277, 1076
CviAII CATG 5 cut(s) 25, 103, 205, 254, 689
CviQI GTAC 2 cut(s) 277, 1076
DdeI CTNAG 6 cut(s) 109, 154, 502, 570, 627, 1079
DpnI GATC 6 cut(s) 168, 215, 288, 662, 687, 894
DpnII GATC 6 cut(s) 166, 213, 286, 660, 685, 892
EaeI YGGCCR 2 cut(s) 40, 967
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 378
Eco47I GGWCC 2 cut(s) 185, 343
Eco57I CTGAAG 1 cut(s) 599
EcoO109I RGGNCCY 2 cut(s) 185, 343
EcoRI GAATTC 1 cut(s) 266
EcoRII CCWGG 2 cut(s) 228, 400
FaeI CATG 5 cut(s) 28, 106, 208, 257, 692
FalI AAGNNNNNCTT 2 cut(s) 584, 616
FatI CATG 5 cut(s) 24, 102, 204, 253, 688
FauNDI CATATG 1 cut(s) 787
Fnu4HI GCNGC 2 cut(s) 258, 679
FokI GGATG 3 cut(s) 17, 442, 796
Fsp4HI GCNGC 2 cut(s) 258, 679
FspBI CTAG 1 cut(s) 480
GluI GCNGC 2 cut(s) 258, 679
GsuI CTGGAG 1 cut(s) 51
HaeIII GGCC 3 cut(s) 42, 681, 969
HapII CCGG 2 cut(s) 491, 683
Hin1II CATG 5 cut(s) 28, 106, 208, 257, 692
HindIII AAGCTT 1 cut(s) 776
HinfI GANTC 3 cut(s) 548, 782, 956
HpaII CCGG 2 cut(s) 491, 683
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 3 cut(s) 277, 467, 538
Hpy188I TCNGA 5 cut(s) 88, 272, 505, 547, 628
Hpy188III TCNNGA 4 cut(s) 183, 205, 642, 953
Hpy8I GTNNAC 3 cut(s) 277, 467, 538
HpyAV CCTTC 5 cut(s) 73, 91, 387, 503, 763
HpyCH4III ACNGT 4 cut(s) 118, 464, 940, 998
HpyCH4V TGCA 8 cut(s) 56, 121, 257, 442, 796, 833, 920, 932
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 254
HpyF3I CTNAG 6 cut(s) 109, 154, 502, 570, 627, 1079
Hsp92II CATG 5 cut(s) 28, 106, 208, 257, 692
Kzo9I GATC 6 cut(s) 166, 213, 286, 660, 685, 892
LmnI GCTCC 5 cut(s) 148, 440, 675, 718, 811
Lsp1109I GCAGC 1 cut(s) 269
LweI GCATC 2 cut(s) 92, 820
MaeI CTAG 1 cut(s) 480
MalI GATC 6 cut(s) 168, 215, 288, 662, 687, 894
MbiI CCGCTC 1 cut(s) 678
MboI GATC 6 cut(s) 166, 213, 286, 660, 685, 892
MfeI CAATTG 1 cut(s) 857
MlsI TGGCCA 2 cut(s) 42, 969
MluCI AATT 5 cut(s) 266, 587, 857, 881, 1035
MluNI TGGCCA 2 cut(s) 42, 969
MlyI GAGTC 1 cut(s) 776
MmeI TCCRAC 1 cut(s) 849
Mox20I TGGCCA 2 cut(s) 42, 969
MroXI GAANNNNTTC 1 cut(s) 587
MscI TGGCCA 2 cut(s) 42, 969
MseI TTAA 2 cut(s) 282, 1053
MslI CAYNNNNRTG 1 cut(s) 801
Msp20I TGGCCA 2 cut(s) 42, 969
MspI CCGG 2 cut(s) 491, 683
MspR9I CCNGG 3 cut(s) 230, 402, 683
MunI CAATTG 1 cut(s) 857
MvaI CCWGG 2 cut(s) 230, 402
MwoI GCNNNNNNNGC 2 cut(s) 48, 254
NciI CCSGG 1 cut(s) 683
NdeI CATATG 1 cut(s) 787
NdeII GATC 6 cut(s) 166, 213, 286, 660, 685, 892
NlaIII CATG 5 cut(s) 28, 106, 208, 257, 692
NlaIV GGNNCC 2 cut(s) 187, 486
PagI TCATGA 1 cut(s) 204
PdmI GAANNNNTTC 1 cut(s) 587
PfeI GAWTC 2 cut(s) 548, 956
PkrI GCNGC 2 cut(s) 259, 680
PleI GAGTC 1 cut(s) 776
PpsI GAGTC 1 cut(s) 776
PpuMI RGGWCCY 2 cut(s) 185, 343
Psp5II RGGWCCY 2 cut(s) 185, 343
Psp6I CCWGG 2 cut(s) 228, 400
PspGI CCWGG 2 cut(s) 228, 400
PspN4I GGNNCC 2 cut(s) 187, 486
PspPI GGNCC 3 cut(s) 185, 343, 680
PspPPI RGGWCCY 2 cut(s) 185, 343
RsaI GTAC 2 cut(s) 278, 1077
RsaNI GTAC 2 cut(s) 277, 1076
RseI CAYNNNNRTG 1 cut(s) 801
SaqAI TTAA 2 cut(s) 282, 1053
SatI GCNGC 2 cut(s) 258, 679
Sau3AI GATC 6 cut(s) 166, 213, 286, 660, 685, 892
Sau96I GGNCC 3 cut(s) 185, 343, 680
SchI GAGTC 1 cut(s) 776
ScrFI CCNGG 3 cut(s) 230, 402, 683
SfaNI GCATC 2 cut(s) 92, 820
SfcI CTRYAG 3 cut(s) 460, 561, 1012
SinI GGWCC 2 cut(s) 185, 343
SmiMI CAYNNNNRTG 1 cut(s) 801
Sse9I AATT 5 cut(s) 266, 587, 857, 881, 1035
SsiI CCGC 1 cut(s) 678
SspMI CTAG 1 cut(s) 480
StyD4I CCNGG 3 cut(s) 228, 400, 681
TaaI ACNGT 4 cut(s) 118, 464, 940, 998
TasI AATT 5 cut(s) 266, 587, 857, 881, 1035
TatI WGTACW 1 cut(s) 1075
TauI GCSGC 1 cut(s) 681
TfiI GAWTC 2 cut(s) 548, 956
Tru1I TTAA 2 cut(s) 282, 1053
Tru9I TTAA 2 cut(s) 282, 1053
TscAI CASTG 4 cut(s) 123, 476, 538, 840
TseI GCWGC 1 cut(s) 257
TspDTI ATGAA 4 cut(s) 221, 242, 374, 444
TspRI CASTG 4 cut(s) 123, 476, 538, 840
VpaK11BI GGWCC 2 cut(s) 185, 343
XapI RAATTY 1 cut(s) 266
XmnI GAANNNNTTC 1 cut(s) 587
XspI CTAG 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.