MD15G1256500.v1.1

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016697
Physical Location & Seq
Forward (+)
21775419 .. 21777379
1961 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1256500.v1.1.491

Sequence Viewer

Length: 1179 bp
ATGCATCATGTGAATTTGCCTTTAAAGTATAAGAAGCAAGGTCCACCAAGTTCAAAGGCAGCAGAGAGCCAGCTGCAACAAGCTAGCATGGGTGTAGAGAATGTCTTCCACATGACTGGAGGAGTTGGCCGTACTAGCTATGCCAGAAATTCTTCACTCCAGAAGAAGGCATCTGATATGGTAAAGAACATAACTTTAGAGGCAATACAAGAACTTTACCTCACCACAACTCCAAAGAGCTTAGGCATAGCTGATTTGGGCTGTTCATCTGGACCCAACACCTTATCAATCATCAAAGAAATCATTGAAGCAGTTGAAGGCTTCAGTATAAAAACATTTCAACCATCACCAGAGTTCAGAGTTCACCTCAATGATCTTCCCTCCAATGACTTCAACTCAATCTTCAAGGCCTTGCCAGACTTCTCCAAAGACCTCAGCGACGAAAGAAGCAATGGGAAATCTCCCTCTATTTATATAGGAGCCTATCCTGGCTCATTTTATGGCAGACTTTTCCCCAACAATTCCTTACACTTTGTCTACTCATCCTACAGTTTGCACTGGCTATCTAGGGTTCCTCCAAAAATTTACTGCAAAGAGGGGAAGTCCACAAACAAAGGAAGTGTTTACATTTCTGAATCAAGCCCTGTTGGAGTTTCTCAAGCTTACCTGAAGCAATTTCAAGAGGACTTCACTTTGTTCCTTAAGTCAAGGTCCGAGGAGCTGGTTGCAGGAGGAAGAATGGTGCTGATCTTGTTGGGAAGGAGTGGCCCAGATCACGTTGATAGAGGCAATTGTTTCCTCTGGCAGCTTCTTTCTCAGTCCATTGCCATTTTGGTTTCCGAGGGTGAAGTTGAGGAGGAAAAGCTTGACTCATATGATGTGCATTTCTATGCTCCATCGAGAGATGAAATAGAACATGAAGTGAGGAAGGAGGGTTCTTTTGCATTGGACAGGTTTGAGATGTTTTGGCTGGATCAGAGGCATGGCAAGAACGATGAGAGCTATGGAACTAGTATTGCAATGACTGTGAGGGCTATTCAAGAATCAATGATTGGGCAGCATTTTGGAGAAGGAATCTTGGACAGTATATTTGAAAATTTTAGGAGATTGGTGGATGAAGAAGCGGCTAAAGAAGAGATTAAGCCCATCACTTTTGGGATTGTACTTAGAAAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

393

Amino Acids

43.98

Weight (kDa)

5.93

Isoelectric Point (pI)

49.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 67 - 391 6.9e-120 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36470
fragaria_vesca FvH4_1g12770
malus_domestica MD15G1256500.v1.1
prunus_persica Prupe.7G158400_v2.0.a1 Prupe.7G158400_v2.0.a1
pyrus_communis pycom02g11320 pycom15g22440
rosa_chinensis RchiOBHm_Chr2g0100891
rosa_laevigata RLG00000017021
rosa_multiflora Rmu_sc0001659.1_g000045
rosa_roxburghii Rroxscaffold_2G00141760
rosa_rugosa Rorug02G0093400
rosa_samantha Rh2BG146100
rosa_wichuraiana Rw2G011060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1177
AccI GTMKAC 1 cut(s) 537
AciI CCGC 1 cut(s) 1124
AclWI GGATC 1 cut(s) 981
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 4 cut(s) 13, 148, 582, 1096
AcuI CTGAAG 2 cut(s) 307, 689
AfaI GTAC 2 cut(s) 133, 1164
AfiI CCNNNNNNNGG 1 cut(s) 166
AflII CTTAAG 1 cut(s) 701
AgsI TTSAA 9 cut(s) 54, 308, 317, 341, 394, 406, 680, 1040, 1094
AhlI ACTAGT 1 cut(s) 1010
AjnI CCWGG 1 cut(s) 487
AjuI GAANNNNNNNTTGG 2 cut(s) 1035, 1067
AloI GAACNNNNNNTCC 2 cut(s) 919, 951
AlwI GGATC 1 cut(s) 981
AoxI GGCC 3 cut(s) 127, 408, 766
ApeKI GCWGC 4 cut(s) 59, 73, 805, 1057
ApoI RAATTY 4 cut(s) 13, 148, 582, 1096
ArsI GACNNNNNNTTYG 2 cut(s) 1073, 1105
Asp700I GAANNNNTTC 2 cut(s) 104, 151
AspS9I GGNCC 4 cut(s) 41, 272, 711, 767
AsuHPI GGTGA 4 cut(s) 214, 339, 356, 857
AsuNHI GCTAGC 1 cut(s) 83
AvaII GGWCC 3 cut(s) 41, 272, 711
BbsI GAAGAC 1 cut(s) 97
BbvCI CCTCAGC 1 cut(s) 434
BbvI GCAGC 4 cut(s) 60, 71, 817, 1069
BccI CCATC 3 cut(s) 352, 904, 1154
BceAI ACGGC 1 cut(s) 114
BciT130I CCWGG 1 cut(s) 489
BcuI ACTAGT 1 cut(s) 1010
BfaI CTAG 4 cut(s) 84, 135, 567, 1011
BfmI CTRYAG 1 cut(s) 547
BfrI CTTAAG 1 cut(s) 701
BisI GCNGC 5 cut(s) 60, 74, 806, 1058, 1125
BlsI GCNGC 5 cut(s) 61, 75, 807, 1059, 1126
Bme1390I CCNGG 1 cut(s) 489
Bme18I GGWCC 3 cut(s) 41, 272, 711
BmgT120I GGNCC 4 cut(s) 41, 272, 711, 767
BmiI GGNNCC 3 cut(s) 274, 481, 573
BmrFI CCNGG 1 cut(s) 489
BmsI GCATC 2 cut(s) 13, 179
BmtI GCTAGC 1 cut(s) 87
BpiI GAAGAC 1 cut(s) 97
BplI GAGNNNNNCTC 2 cut(s) 351, 383
BpmI CTGGAG 2 cut(s) 138, 143
Bpu10I CCTNAGC 2 cut(s) 241, 434
BpuEI CTTGAG 1 cut(s) 642
BsaJI CCNNGG 2 cut(s) 714, 840
BsaXI ACNNNNNCTCC 2 cut(s) 214, 244
Bsc4I CCNNNNNNNGG 1 cut(s) 166
Bse1I ACTGG 2 cut(s) 121, 563
Bse3DI GCAATG 3 cut(s) 457, 822, 1026
BseBI CCWGG 1 cut(s) 489
BseDI CCNNGG 2 cut(s) 714, 840
BseGI GGATG 2 cut(s) 542, 1120
BseLI CCNNNNNNNGG 1 cut(s) 166
BseMI GCAATG 3 cut(s) 457, 822, 1026
BseMII CTCAG 2 cut(s) 448, 830
BseNI ACTGG 2 cut(s) 121, 563
BseRI GAGGAG 3 cut(s) 135, 731, 869
BseXI GCAGC 4 cut(s) 60, 71, 817, 1069
BshFI GGCC 3 cut(s) 129, 410, 768
BslI CCNNNNNNNGG 1 cut(s) 166
BsnI GGCC 3 cut(s) 129, 410, 768
Bsp143I GATC 4 cut(s) 373, 747, 772, 973
BspACI CCGC 1 cut(s) 1124
BspANI GGCC 3 cut(s) 129, 410, 768
BspCNI CTCAG 2 cut(s) 447, 829
BspLI GGNNCC 3 cut(s) 274, 481, 573
BspOI GCTAGC 1 cut(s) 87
BspPI GGATC 1 cut(s) 981
BspTI CTTAAG 1 cut(s) 701
BsrDI GCAATG 3 cut(s) 457, 822, 1026
BsrI ACTGG 2 cut(s) 121, 563
BssECI CCNNGG 2 cut(s) 714, 840
BssMI GATC 4 cut(s) 373, 747, 772, 973
Bst2UI CCWGG 1 cut(s) 489
Bst4CI ACNGT 3 cut(s) 551, 1027, 1085
Bst6I CTCTTC 1 cut(s) 1128
BstAFI CTTAAG 1 cut(s) 701
BstC8I GCNNGC 2 cut(s) 71, 85
BstDEI CTNAG 4 cut(s) 241, 434, 816, 1166
BstF5I GGATG 2 cut(s) 542, 1120
BstKTI GATC 4 cut(s) 376, 750, 775, 976
BstMBI GATC 4 cut(s) 373, 747, 772, 973
BstMWI GCNNNNNNNGC 1 cut(s) 135
BstNI CCWGG 1 cut(s) 489
BstSCI CCNGG 1 cut(s) 487
BstSFI CTRYAG 1 cut(s) 547
BstV1I GCAGC 4 cut(s) 60, 71, 817, 1069
BstV2I GAAGAC 1 cut(s) 97
BstXI CCANNNNNNTGG 1 cut(s) 116
BsuRI GGCC 3 cut(s) 129, 410, 768
BtsCI GGATG 2 cut(s) 542, 1120
BtsIMutI CAGTG 1 cut(s) 556
Cac8I GCNNGC 2 cut(s) 71, 85
Cfr13I GGNCC 4 cut(s) 41, 272, 711, 767
Csp6I GTAC 2 cut(s) 132, 1163
CviAII CATG 5 cut(s) 8, 88, 112, 917, 983
CviQI GTAC 2 cut(s) 132, 1163
DdeI CTNAG 4 cut(s) 241, 434, 816, 1166
DpnI GATC 4 cut(s) 375, 749, 774, 975
DpnII GATC 4 cut(s) 373, 747, 772, 973
DraI TTTAAA 1 cut(s) 24
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 1 cut(s) 1128
EarI CTCTTC 1 cut(s) 1128
Eco147I AGGCCT 1 cut(s) 410
Eco47I GGWCC 3 cut(s) 41, 272, 711
Eco57I CTGAAG 2 cut(s) 307, 689
EcoRII CCWGG 1 cut(s) 487
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 5 cut(s) 11, 91, 115, 920, 986
FatI CATG 5 cut(s) 7, 87, 111, 916, 982
FauNDI CATATG 1 cut(s) 874
FblI GTMKAC 1 cut(s) 537
Fnu4HI GCNGC 5 cut(s) 60, 74, 806, 1058, 1125
FokI GGATG 2 cut(s) 529, 1127
Fsp4HI GCNGC 5 cut(s) 60, 74, 806, 1058, 1125
FspBI CTAG 4 cut(s) 84, 135, 567, 1011
GluI GCNGC 5 cut(s) 60, 74, 806, 1058, 1125
GsuI CTGGAG 2 cut(s) 138, 143
HaeIII GGCC 3 cut(s) 129, 410, 768
Hin1II CATG 5 cut(s) 11, 91, 115, 920, 986
HindIII AAGCTT 2 cut(s) 660, 863
HinfI GANTC 4 cut(s) 635, 869, 1043, 1074
HphI GGTGA 4 cut(s) 214, 339, 356, 857
Hpy166II GTNNAC 5 cut(s) 44, 364, 538, 606, 625
Hpy188I TCNGA 6 cut(s) 175, 359, 634, 715, 841, 978
Hpy188III TCNNGA 5 cut(s) 160, 270, 680, 900, 1040
Hpy8I GTNNAC 5 cut(s) 44, 364, 538, 606, 625
Hpy99I CGWCG 1 cut(s) 443
HpyAV CCTTC 5 cut(s) 160, 311, 753, 922, 1064
HpyCH4III ACNGT 3 cut(s) 551, 1027, 1085
HpyCH4IV ACGT 1 cut(s) 777
HpyCH4V TGCA 8 cut(s) 4, 76, 556, 591, 728, 883, 944, 1019
HpyF10VI GCNNNNNNNGC 1 cut(s) 135
HpyF3I CTNAG 4 cut(s) 241, 434, 816, 1166
HpySE526I ACGT 1 cut(s) 777
Hsp92II CATG 5 cut(s) 11, 91, 115, 920, 986
Kzo9I GATC 4 cut(s) 373, 747, 772, 973
LmnI GCTCC 3 cut(s) 479, 718, 898
Lsp1109I GCAGC 4 cut(s) 60, 71, 817, 1069
LweI GCATC 2 cut(s) 13, 179
MaeI CTAG 4 cut(s) 84, 135, 567, 1011
MaeII ACGT 1 cut(s) 777
MalI GATC 4 cut(s) 375, 749, 774, 975
MboI GATC 4 cut(s) 373, 747, 772, 973
MboII GAAGA 8 cut(s) 97, 144, 175, 368, 394, 747, 1130, 1145
MfeI CAATTG 1 cut(s) 790
MluCI AATT 8 cut(s) 13, 148, 520, 582, 674, 790, 1096, 1172
MlyI GAGTC 1 cut(s) 863
MmeI TCCRAC 1 cut(s) 628
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 104, 151
MseI TTAA 3 cut(s) 23, 702, 1140
MslI CAYNNNNRTG 2 cut(s) 369, 888
MspA1I CMGCKG 1 cut(s) 73
MspCI CTTAAG 1 cut(s) 701
MspR9I CCNGG 1 cut(s) 489
MunI CAATTG 1 cut(s) 790
MvaI CCWGG 1 cut(s) 489
MwoI GCNNNNNNNGC 1 cut(s) 135
NdeI CATATG 1 cut(s) 874
NdeII GATC 4 cut(s) 373, 747, 772, 973
NheI GCTAGC 1 cut(s) 83
NlaIII CATG 5 cut(s) 11, 91, 115, 920, 986
NlaIV GGNNCC 3 cut(s) 274, 481, 573
NsiI ATGCAT 1 cut(s) 6
PceI AGGCCT 1 cut(s) 410
PdmI GAANNNNTTC 2 cut(s) 104, 151
PfeI GAWTC 3 cut(s) 635, 1043, 1074
PkrI GCNGC 5 cut(s) 61, 75, 807, 1059, 1126
PleI GAGTC 1 cut(s) 863
PpsI GAGTC 1 cut(s) 863
PsiI TTATAA 1 cut(s) 1177
Psp6I CCWGG 1 cut(s) 487
PspGI CCWGG 1 cut(s) 487
PspN4I GGNNCC 3 cut(s) 274, 481, 573
PspPI GGNCC 4 cut(s) 41, 272, 711, 767
PvuII CAGCTG 1 cut(s) 73
RsaI GTAC 2 cut(s) 133, 1164
RsaNI GTAC 2 cut(s) 132, 1163
RseI CAYNNNNRTG 2 cut(s) 369, 888
SaqAI TTAA 3 cut(s) 23, 702, 1140
SatI GCNGC 5 cut(s) 60, 74, 806, 1058, 1125
Sau3AI GATC 4 cut(s) 373, 747, 772, 973
Sau96I GGNCC 4 cut(s) 41, 272, 711, 767
SchI GAGTC 1 cut(s) 863
ScrFI CCNGG 1 cut(s) 489
SfaNI GCATC 2 cut(s) 13, 179
SfcI CTRYAG 1 cut(s) 547
SinI GGWCC 3 cut(s) 41, 272, 711
SmiMI CAYNNNNRTG 2 cut(s) 369, 888
SmlI CTYRAG 2 cut(s) 657, 701
SmoI CTYRAG 2 cut(s) 657, 701
SpeI ACTAGT 1 cut(s) 1010
Sse9I AATT 8 cut(s) 13, 148, 520, 582, 674, 790, 1096, 1172
SseBI AGGCCT 1 cut(s) 410
SsiI CCGC 1 cut(s) 1124
SspMI CTAG 4 cut(s) 84, 135, 567, 1011
StuI AGGCCT 1 cut(s) 410
StyD4I CCNGG 1 cut(s) 487
TaaI ACNGT 3 cut(s) 551, 1027, 1085
TaiI ACGT 1 cut(s) 780
TaqI TCGA 1 cut(s) 899
TasI AATT 8 cut(s) 13, 148, 520, 582, 674, 790, 1096, 1172
TatI WGTACW 1 cut(s) 1162
TauI GCSGC 1 cut(s) 1127
TfiI GAWTC 3 cut(s) 635, 1043, 1074
Tru1I TTAA 3 cut(s) 23, 702, 1140
Tru9I TTAA 3 cut(s) 23, 702, 1140
TscAI CASTG 1 cut(s) 563
TseI GCWGC 4 cut(s) 59, 73, 805, 1057
TspDTI ATGAA 4 cut(s) 255, 921, 933, 1131
TspRI CASTG 1 cut(s) 563
Vha464I CTTAAG 1 cut(s) 701
VpaK11BI GGWCC 3 cut(s) 41, 272, 711
XapI RAATTY 4 cut(s) 13, 148, 582, 1096
XcmI CCANNNNNNNNNTGG 1 cut(s) 829
XmiI GTMKAC 1 cut(s) 537
XmnI GAANNNNTTC 2 cut(s) 104, 151
XspI CTAG 4 cut(s) 84, 135, 567, 1011
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.