pycom15g22440

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016697
Physical Location & Seq
Forward (+)
16571571 .. 16573499
1929 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g22440.4

Sequence Viewer

Length: 1179 bp
ATGCATCATGTGAATTTGCCTTCAAAATATAAGAAGCAAGCTCCACCAAGTTCGAAGGCAGCAGAGAACCAGCTGCAACAAGCTAGCATGGGTGTAGAGAATGTCTTCCACATGACTGGAGGAGTTGGCCGTACAAGCTATGCCAGAAATTCTTCACTCCAGAAGAAGGCATCTGATATGGTAAAGAACATAACTTTAGAGGCAATACAAGAACTTTACCTCACAACAACTCCAAAAAGCTTAGGCGTAGCTGATTTGGGCTGTTCATCCGGACCCAACACCTTATCAATCATCAAAGAAATCATTGAAGCAGTTGAAGGCTTCAGTATAAAAACATTTCAACCATCACCAGAGTTCAGAGTTCACCTCAATGATCTTCCCTCCAATGACTTCAACTCAATCTTCAAGGCCTTGCCAGACTTCTCCAAAGACCTCAGCGACGAAAGAAGTAATGGGAAATCTCCCTCTATTTATATAGGAGCCTATCCTGGCTCATTTTATGGAAGACTTTTTCCCAACAATACATTGCACTTTGTCTACTCATCCTACAGTTTGCACTGGCTATCTAGGGTTCCTCCAAAAATTTACTGCAAAGAGGGGAAGTCTATAAACAAAGGAAGTGTTTACATTTCTGAATCAAGCCCTGTTGGAGTTTCTCAAGCTTACCTGAAGCAATTTCAAGAGGACTTCACTTTGTTCCTTAAGTCAAGGTCTGAGGAGCTGACTGCAGGAGGAAGAATGGTGCTGATCTTGTTGGGAAGGAGTGGCCCGGATCATGTTGATAGAGGCAATTGTTTCTTATGGCAGCTTCTTTCTCAGTCCATTGCCATTTTGGTTTCCGAGGGTGAAGTTGAGGAGGAAAAGCTTGACTCATATGATGTGCATTTCTATGCTCCATCGAGAGATGAAATAGAACAGGAAGTGAGGAAGGAGGGTTCTTTTGAATTGGACAGGTTTGAGATGTTTTGGCTGGATGAGAGGCATGGCAAGAACGATGAGAGCTATGGAACTAGTATTGCAATGACTGTGAGGGCTATTCAAGAACCAATGATTGGGCAGCATTTTGGAGAAGGAATCTTGGACAGTTTGTTTGAAAATTTTGGGAGATTGGTGGATGAAGAAGCGGCTAAAGAAGAGATTAAGCCCATCACTTTTGGGATTGTACTTAGAAAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

393

Amino Acids

43.97

Weight (kDa)

5.58

Isoelectric Point (pI)

49.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016697)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36470
fragaria_vesca FvH4_1g12770
malus_domestica MD15G1256500.v1.1
prunus_persica Prupe.7G158400_v2.0.a1 Prupe.7G158400_v2.0.a1
pyrus_communis pycom02g11320 pycom15g22440
rosa_chinensis RchiOBHm_Chr2g0100891
rosa_laevigata RLG00000017021
rosa_multiflora Rmu_sc0001659.1_g000045
rosa_roxburghii Rroxscaffold_2G00141760
rosa_rugosa Rorug02G0093400
rosa_samantha Rh2BG146100
rosa_wichuraiana Rw2G011060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1052
AccI GTMKAC 1 cut(s) 537
AccIII TCCGGA 1 cut(s) 269
AciI CCGC 1 cut(s) 1124
AclWI GGATC 1 cut(s) 780
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 4 cut(s) 13, 148, 582, 1096
AcuI CTGAAG 2 cut(s) 307, 689
AfaI GTAC 2 cut(s) 133, 1164
AfiI CCNNNNNNNGG 2 cut(s) 166, 1052
AflII CTTAAG 1 cut(s) 701
AhlI ACTAGT 1 cut(s) 1010
AjnI CCWGG 1 cut(s) 487
AjuI GAANNNNNNNTTGG 2 cut(s) 1035, 1067
AloI GAACNNNNNNTCC 2 cut(s) 919, 951
AlwI GGATC 1 cut(s) 780
Aor13HI TCCGGA 1 cut(s) 269
AoxI GGCC 3 cut(s) 127, 408, 766
ApeKI GCWGC 4 cut(s) 59, 73, 805, 1057
ApoI RAATTY 4 cut(s) 13, 148, 582, 1096
ArsI GACNNNNNNTTYG 2 cut(s) 1073, 1105
Asp700I GAANNNNTTC 2 cut(s) 104, 151
AspS9I GGNCC 2 cut(s) 272, 767
AsuC2I CCSGG 1 cut(s) 770
AsuHPI GGTGA 3 cut(s) 339, 356, 857
AsuII TTCGAA 1 cut(s) 53
AsuNHI GCTAGC 1 cut(s) 83
AvaII GGWCC 1 cut(s) 272
BbsI GAAGAC 2 cut(s) 97, 511
BbvCI CCTCAGC 1 cut(s) 434
BbvI GCAGC 4 cut(s) 60, 71, 817, 1069
BccI CCATC 3 cut(s) 352, 904, 1154
BceAI ACGGC 1 cut(s) 114
BciT130I CCWGG 1 cut(s) 489
BcnI CCSGG 1 cut(s) 770
BcuI ACTAGT 1 cut(s) 1010
BfaI CTAG 3 cut(s) 84, 567, 1011
BfmI CTRYAG 2 cut(s) 547, 726
BfrI CTTAAG 1 cut(s) 701
BisI GCNGC 5 cut(s) 60, 74, 806, 1058, 1125
BlsI GCNGC 5 cut(s) 61, 75, 807, 1059, 1126
Bme1390I CCNGG 2 cut(s) 489, 770
Bme18I GGWCC 1 cut(s) 272
BmgT120I GGNCC 2 cut(s) 272, 767
BmiI GGNNCC 3 cut(s) 274, 481, 573
BmrFI CCNGG 2 cut(s) 489, 770
BmsI GCATC 2 cut(s) 13, 179
BmtI GCTAGC 1 cut(s) 87
BpiI GAAGAC 2 cut(s) 97, 511
BplI GAGNNNNNCTC 2 cut(s) 351, 383
BpmI CTGGAG 2 cut(s) 138, 143
Bpu10I CCTNAGC 2 cut(s) 241, 434
Bpu14I TTCGAA 1 cut(s) 53
BpuEI CTTGAG 1 cut(s) 642
BpuMI CCSGG 1 cut(s) 770
BsaJI CCNNGG 1 cut(s) 840
BsaWI WCCGGW 1 cut(s) 269
BsaXI ACNNNNNCTCC 2 cut(s) 214, 244
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 1052
Bse1I ACTGG 2 cut(s) 121, 563
Bse3DI GCAATG 3 cut(s) 524, 822, 1026
BseAI TCCGGA 1 cut(s) 269
BseBI CCWGG 1 cut(s) 489
BseDI CCNNGG 1 cut(s) 840
BseGI GGATG 4 cut(s) 266, 542, 979, 1120
BseLI CCNNNNNNNGG 2 cut(s) 166, 1052
BseMI GCAATG 3 cut(s) 524, 822, 1026
BseMII CTCAG 3 cut(s) 448, 705, 830
BseNI ACTGG 2 cut(s) 121, 563
BseRI GAGGAG 3 cut(s) 135, 731, 869
BseXI GCAGC 4 cut(s) 60, 71, 817, 1069
BshFI GGCC 3 cut(s) 129, 410, 768
BsiSI CCGG 2 cut(s) 270, 770
BslI CCNNNNNNNGG 2 cut(s) 166, 1052
BsnI GGCC 3 cut(s) 129, 410, 768
Bsp119I TTCGAA 1 cut(s) 53
Bsp13I TCCGGA 1 cut(s) 269
Bsp143I GATC 3 cut(s) 373, 747, 772
BspACI CCGC 1 cut(s) 1124
BspANI GGCC 3 cut(s) 129, 410, 768
BspCNI CTCAG 3 cut(s) 447, 706, 829
BspEI TCCGGA 1 cut(s) 269
BspLI GGNNCC 3 cut(s) 274, 481, 573
BspMAI CTGCAG 1 cut(s) 730
BspOI GCTAGC 1 cut(s) 87
BspPI GGATC 1 cut(s) 780
BspT104I TTCGAA 1 cut(s) 53
BspTI CTTAAG 1 cut(s) 701
BsrDI GCAATG 3 cut(s) 524, 822, 1026
BsrI ACTGG 2 cut(s) 121, 563
BssECI CCNNGG 1 cut(s) 840
BssMI GATC 3 cut(s) 373, 747, 772
Bst2UI CCWGG 1 cut(s) 489
Bst4CI ACNGT 3 cut(s) 551, 1027, 1085
Bst6I CTCTTC 1 cut(s) 1128
BstAFI CTTAAG 1 cut(s) 701
BstBI TTCGAA 1 cut(s) 53
BstC8I GCNNGC 2 cut(s) 39, 85
BstDEI CTNAG 5 cut(s) 241, 434, 714, 816, 1166
BstF5I GGATG 4 cut(s) 266, 542, 979, 1120
BstKTI GATC 3 cut(s) 376, 750, 775
BstMBI GATC 3 cut(s) 373, 747, 772
BstMWI GCNNNNNNNGC 1 cut(s) 135
BstNI CCWGG 1 cut(s) 489
BstSCI CCNGG 2 cut(s) 487, 768
BstSFI CTRYAG 2 cut(s) 547, 726
BstV1I GCAGC 4 cut(s) 60, 71, 817, 1069
BstV2I GAAGAC 2 cut(s) 97, 511
BstXI CCANNNNNNTGG 1 cut(s) 116
BsuRI GGCC 3 cut(s) 129, 410, 768
BtsCI GGATG 4 cut(s) 266, 542, 979, 1120
BtsIMutI CAGTG 1 cut(s) 556
Cac8I GCNNGC 2 cut(s) 39, 85
Cfr13I GGNCC 2 cut(s) 272, 767
Csp6I GTAC 2 cut(s) 132, 1163
CviAII CATG 5 cut(s) 8, 88, 112, 776, 983
CviQI GTAC 2 cut(s) 132, 1163
DdeI CTNAG 5 cut(s) 241, 434, 714, 816, 1166
DpnI GATC 3 cut(s) 375, 749, 774
DpnII GATC 3 cut(s) 373, 747, 772
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 1 cut(s) 1128
EarI CTCTTC 1 cut(s) 1128
Eco147I AGGCCT 1 cut(s) 410
Eco47I GGWCC 1 cut(s) 272
Eco57I CTGAAG 2 cut(s) 307, 689
EcoRII CCWGG 1 cut(s) 487
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 5 cut(s) 11, 91, 115, 779, 986
FatI CATG 5 cut(s) 7, 87, 111, 775, 982
FauNDI CATATG 1 cut(s) 874
FblI GTMKAC 1 cut(s) 537
Fnu4HI GCNGC 5 cut(s) 60, 74, 806, 1058, 1125
FokI GGATG 4 cut(s) 253, 529, 986, 1127
Fsp4HI GCNGC 5 cut(s) 60, 74, 806, 1058, 1125
FspBI CTAG 3 cut(s) 84, 567, 1011
GluI GCNGC 5 cut(s) 60, 74, 806, 1058, 1125
GsuI CTGGAG 2 cut(s) 138, 143
HaeIII GGCC 3 cut(s) 129, 410, 768
HapII CCGG 2 cut(s) 270, 770
Hin1II CATG 5 cut(s) 11, 91, 115, 779, 986
HindIII AAGCTT 3 cut(s) 238, 660, 863
HinfI GANTC 3 cut(s) 635, 869, 1074
HpaII CCGG 2 cut(s) 270, 770
HphI GGTGA 3 cut(s) 339, 356, 857
Hpy166II GTNNAC 3 cut(s) 364, 538, 625
Hpy188I TCNGA 5 cut(s) 175, 359, 634, 715, 841
Hpy188III TCNNGA 5 cut(s) 160, 270, 680, 900, 1040
Hpy8I GTNNAC 3 cut(s) 364, 538, 625
Hpy99I CGWCG 1 cut(s) 443
HpyAV CCTTC 7 cut(s) 30, 49, 160, 311, 753, 922, 1064
HpyCH4III ACNGT 3 cut(s) 551, 1027, 1085
HpyCH4V TGCA 8 cut(s) 4, 76, 529, 556, 591, 728, 883, 1019
HpyF10VI GCNNNNNNNGC 1 cut(s) 135
HpyF3I CTNAG 5 cut(s) 241, 434, 714, 816, 1166
Hsp92II CATG 5 cut(s) 11, 91, 115, 779, 986
Kpn2I TCCGGA 1 cut(s) 269
Kzo9I GATC 3 cut(s) 373, 747, 772
LmnI GCTCC 4 cut(s) 46, 479, 718, 898
Lsp1109I GCAGC 4 cut(s) 60, 71, 817, 1069
LweI GCATC 2 cut(s) 13, 179
MaeI CTAG 3 cut(s) 84, 567, 1011
MalI GATC 3 cut(s) 375, 749, 774
MboI GATC 3 cut(s) 373, 747, 772
MboII GAAGA 9 cut(s) 97, 144, 175, 368, 394, 516, 747, 1130, 1145
MfeI CAATTG 1 cut(s) 790
MluCI AATT 8 cut(s) 13, 148, 582, 674, 790, 944, 1096, 1172
MlyI GAGTC 1 cut(s) 863
MmeI TCCRAC 1 cut(s) 628
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 269
MroXI GAANNNNTTC 2 cut(s) 104, 151
MseI TTAA 2 cut(s) 702, 1140
MslI CAYNNNNRTG 2 cut(s) 369, 888
MspA1I CMGCKG 1 cut(s) 73
MspCI CTTAAG 1 cut(s) 701
MspI CCGG 2 cut(s) 270, 770
MspR9I CCNGG 2 cut(s) 489, 770
MunI CAATTG 1 cut(s) 790
MvaI CCWGG 1 cut(s) 489
MwoI GCNNNNNNNGC 1 cut(s) 135
NciI CCSGG 1 cut(s) 770
NdeI CATATG 1 cut(s) 874
NdeII GATC 3 cut(s) 373, 747, 772
NheI GCTAGC 1 cut(s) 83
NlaIII CATG 5 cut(s) 11, 91, 115, 779, 986
NlaIV GGNNCC 3 cut(s) 274, 481, 573
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 53
PceI AGGCCT 1 cut(s) 410
PdmI GAANNNNTTC 2 cut(s) 104, 151
PfeI GAWTC 2 cut(s) 635, 1074
PflMI CCANNNNNTGG 1 cut(s) 1052
PkrI GCNGC 5 cut(s) 61, 75, 807, 1059, 1126
PleI GAGTC 1 cut(s) 863
PpsI GAGTC 1 cut(s) 863
Psp6I CCWGG 1 cut(s) 487
PspGI CCWGG 1 cut(s) 487
PspN4I GGNNCC 3 cut(s) 274, 481, 573
PspPI GGNCC 2 cut(s) 272, 767
PstI CTGCAG 1 cut(s) 730
PvuII CAGCTG 1 cut(s) 73
RsaI GTAC 2 cut(s) 133, 1164
RsaNI GTAC 2 cut(s) 132, 1163
RseI CAYNNNNRTG 2 cut(s) 369, 888
SaqAI TTAA 2 cut(s) 702, 1140
SatI GCNGC 5 cut(s) 60, 74, 806, 1058, 1125
Sau3AI GATC 3 cut(s) 373, 747, 772
Sau96I GGNCC 2 cut(s) 272, 767
SchI GAGTC 1 cut(s) 863
ScrFI CCNGG 2 cut(s) 489, 770
SfaNI GCATC 2 cut(s) 13, 179
SfcI CTRYAG 2 cut(s) 547, 726
SfuI TTCGAA 1 cut(s) 53
SinI GGWCC 1 cut(s) 272
SmiMI CAYNNNNRTG 2 cut(s) 369, 888
SmlI CTYRAG 2 cut(s) 657, 701
SmoI CTYRAG 2 cut(s) 657, 701
SpeI ACTAGT 1 cut(s) 1010
Sse9I AATT 8 cut(s) 13, 148, 582, 674, 790, 944, 1096, 1172
SseBI AGGCCT 1 cut(s) 410
SsiI CCGC 1 cut(s) 1124
SspMI CTAG 3 cut(s) 84, 567, 1011
StuI AGGCCT 1 cut(s) 410
StyD4I CCNGG 2 cut(s) 487, 768
TaaI ACNGT 3 cut(s) 551, 1027, 1085
TaqI TCGA 2 cut(s) 53, 899
TasI AATT 8 cut(s) 13, 148, 582, 674, 790, 944, 1096, 1172
TatI WGTACW 1 cut(s) 1162
TauI GCSGC 1 cut(s) 1127
TfiI GAWTC 2 cut(s) 635, 1074
Tru1I TTAA 2 cut(s) 702, 1140
Tru9I TTAA 2 cut(s) 702, 1140
TscAI CASTG 1 cut(s) 563
TseI GCWGC 4 cut(s) 59, 73, 805, 1057
TspDTI ATGAA 3 cut(s) 255, 921, 1131
TspRI CASTG 1 cut(s) 563
Van91I CCANNNNNTGG 1 cut(s) 1052
Vha464I CTTAAG 1 cut(s) 701
VpaK11BI GGWCC 1 cut(s) 272
XapI RAATTY 4 cut(s) 13, 148, 582, 1096
XcmI CCANNNNNNNNNTGG 1 cut(s) 829
XmiI GTMKAC 1 cut(s) 537
XmnI GAANNNNTTC 2 cut(s) 104, 151
XspI CTAG 3 cut(s) 84, 567, 1011
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.