Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0009569

Physical Location & Seq

Genomic Coordinates Reverse (-)

7299003 .. 7304134

Gene Structure 5132 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g13311.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3018 bp

ATGGTTCACAGCGCCTACGATTCCGTCGAGCTCATCTCCGACTGCCCCACCAAGATCGAAGCCATCTCATCCTACGGCCCTAAGCTCCTCCTCGGCTGCTCCGACGGATCCCTCAAAATCTACGCTCCTGATTCCTCCGGCTCCCGCTCCCCTCCCTCCGACTACCACTCCCAGTCCCTCCAAAAGGAGCCCTACTCCCTCGAGCGCAACCTCGCCGGCTTCTCCAAGAAGCCGCTCCTCTCTCTCGAGGTCCTCCAATCCCGAGACATCCTCCTCTCCCTCTCCGAAACGATATCGTTTCACGGCCTCCCCAATCTCGGCACCATCGCCGTCATCACCAAGGCCAAGGGCGCCAATGTGTACTCCTGGGACGACCGCCGCGGCTTCCTCTGCTTCTCGCGCCAGAAGAAGGTCTGCATTTTCCGGCACGACGGTGGGAGAGGTTTCGTGGAGGTGAAGGAATTCGGAGTTCCGGATGTGGTGAAGTCTATGGCGTGGTGTGGAGAGAATATATGTATAGGGATCAGGAGAGATTATATGATACTGAACTCTACAACTGGTGCATTGACTGATGTGTTTCCTTCGGGGAGGCTCGCGCCGCCGTTGGTGGTTCCTCTTCCTTCGGGGGAGCTTCTTCTCAGCAAGGATAACATTGGGGTTTTTGTGGACCAAAATGGGAAGCTTGCTCATGAAGGCAGGGTTTGTTGGACGGAGGCGCCTACGGTTGTTGTTATTCAGAAGGCCTATGGAATAGCTCTGTTATCGAGATATGTTGAGGTTCGGTCCCTACGAGCGCCATATCCGTTGATACAAACAATTATTCTTCGAAATGCTCGTCGTCTTCTCCAAAGCAACAATGCTGCCATTGTTGCATTAGACTATGCTGTTTATGGTCTCTTCCCTGTCCCTCTTGGTGCGCAGATTGTACAATTAACAGCATCTGGTGAGTTTGAGGAAGCTTTGTCATTGTGTAAGCTGCTTCCGCCTGAAGAAGCAAGCCATAGAGCCGCAAAGGAGGCATCAATCCATATCAGATGTGCTCACCATCGTTTTGATTCTGGGGACTATGAGGATGCCATGGAGCATTTTGTGGCTTCACAAGTGGATATAACCTATGTACTTTCTATGTATCCTTCTATTGTTCTTCCCAAGACAACCATGGTAGTTGATCCAGACAAACTGATGGATATATCTACAGACTCATCATATCTCTCAAGAGGTTCATCTGGTATGTCAGATGATATGGAACCCTCTCCCCTCTCACATGTGCTGGAATCGGAGGAGAGTGCAGCACTTGAGTCCAAAAAAATGAGCCATAACACTCTCATGGCTCTCATTAAGTTCTTGCAGAAAAAGAGATTCAGCATAATTGAAAAAGCTACTGCTGAGGGGACAGAAGAGGTTGTTTTGGATGCTGTTGGAGACCGTGAGTCTAATAGGTTCATGAAAATAAACAAGGGGCGTGGCAGCACTCCAGTTACCTCCCGTGCTAGGGAAATGGCAGCTATACTGGATACAGCTCTCCTCCAAGCTCTACTTCTTACTGGACAGTCTTCAGTGGCCTTGGAATTACTGAAAGGTCTCAACTACTGTGATGTAAAAATATGTGAGGAGATTCTCCTAAAAAGTAATCATCATGCTGCTTTGTTAGAACTTTACAAGTGCAATTCAATGCACCATGAAGCTCTAAAACTATTGCAGCAGTTAGTAGAAGAGTCAAAATCTAATCAGGTCCAACCTGAGGTAATCCAAAAGATAAAGCCGGAGTCAATTGTTGAATATCTCAAGCCTCTTTGTGGCACTGATCCCATGCTCGTCTTGGAGTATTCGATGCTTGTCCTTGAGAGTTGCCCAACACAGACAATTGAGCTCTTTCTGAATGGAAATATCCCAGCCGATTTAGTAAACTCTTATTTGAAGCAGCATGCTCCAAACATGCAGGCCAGATACTTAGAACTTATGCTTGCTATGGATGAAAATGGAATCTCAGGGAATCTGCAGAATGAAATGGTGCATATCTATCTATCAGAAGTACTTGACTGGTATGCAGATTTAAGTGCTCAACAGAAATGGGAGGAGCAAACTTACTCCCCTACGAGAAAGAAACTATTGTCTGCTTTAGAAAGCATATCGGGTTATAGTCCGGAGGCTTTGCTGAAACGTCTTCCGGCTGATGCTTTATATGAAGAACGTGCAGTTTTGTTGGGCAAAATGAATCAACATGAGCTTGCCTTGTCCCTATATGTTCACAAGCTTCATCTTCCTGAGATGGCACTGTCCTATTGTGATCGAGTATATGACTCATTGGCGCATCAACCGTCTTCAAGATCTTCTGGCAATATATACCTTACTCTATTACAAATCTATCTTAATCCGAAGAGAACAACCAAGAACTTTGAGAGGCGGATTATGAATTTGGTATCACCGCAGAACAAAGGAACGCCAAAGGTTGGTTCTGCAAATACAGTTAAAAGTAAAGGAGGTCGTGGAGCTAAGAAAATTGCTGCAATAGAGGTGGCAGATGACATAAGAATCAGTCAAAGTGGCACTGACAGTAGTAGGAGTGATGGAGATGCAGATGCAGACGCAGACGAATCTGGTGAGGAAGGAGGGTCAACAATCATGCTTGATGAGGTTCTTGATGTGTTGAGCAGAAAGTGGGACAGAATAAATGGAGCTCAAGCTCTCAAACTTTTGCCTAGGGAGACTAAACTACAGAACTTGCTTCCGTTTATGGGACCCCTTTTGAGAAAATCAAGTGAAGCTTACAGGAATCTTTCAGTGATAAAAAGTTTAAGACAAAGTGATAACCTGCAGGTTAAAGAAGAACTCTACGAACAAAGGAAAGGGGTGGTAAAGATCACAAGCGACAGTGTGTGCTCCCTTTGCAGAAAGAAAATAGGAACAAGTGTGTTTGCTGTGTATCCAAATGGGAGTACAATAGTGCATTTTGTTTGCTTCAAAGACTCCCAAAGTATGAAAGCCGTGGGCAGAGGATCTCCCCTAAGGAAGCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0000325 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005770 GO:0005773 GO:0005774 GO:0005911 GO:0006810 GO:0006906 GO:0006914 GO:0006996 GO:0007033 GO:0007034 GO:0007041 GO:0008150 GO:0008152 GO:0008333 GO:0009056 GO:0009506 GO:0009705 GO:0009987 GO:0010008 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016197 GO:0030054 GO:0030897 GO:0031090 GO:0031410 GO:0031902 GO:0031982 GO:0032991 GO:0034058 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044248 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048284 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055044 GO:0061024 GO:0061025 GO:0061919 GO:0071840 GO:0090174 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:0098876 GO:0099023 GO:1902774 GO:1990126

KEGG Pathways

Metabolic & Signaling

ko04138 map04138

Pfam Domains

Protein Families

CNH Clathrin Vps39_1 Vps39_2

Protein Analysis

1006

Amino Acids

111.27

Weight (kDa)

6.67

Isoelectric Point (pI)

48.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CNH	PF00780	28 - 280	8.5e-20	CNH domain
Clathrin	PF00637	501 - 595	1.9e-06	Region in Clathrin and VPS
Vps39_1	PF10366	504 - 614	3.4e-25	Vacuolar sorting protein 39 domain 1
zf-Vps39_C	PF10367	951 - 989	3e-17	Vacuolar sorting protein 39 C-terminal zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009569)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36630 AT4G36630
fragaria_vesca	FvH4_1g13311
malus_domestica	MD02G1147600.v1.1 MD15G1261900.v1.1
prunus_persica	Prupe.7G154500_v2.0.a1
pyrus_communis	pycom02g11660 pycom15g22910
rosa_chinensis	RchiOBHm_Chr2g0101111 RchiOBHm_Chr2g0101581
rosa_laevigata	RLG00000017092
rosa_multiflora	Rmu_sc0006153.1_g000006 Rmu_sc0019613.1_g000003
rosa_roxburghii	Rroxscaffold_2G00141170
rosa_rugosa	Rorug02G0097300 Rorug02G0100900
rosa_samantha	Rh2AG148200 Rh2BG153600 Rh2CG149800 Rh2CG153900 Rh2DG153900
rosa_wichuraiana	Rw2G011560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	918
Acc36I	ACCTGC	2 cut(s)	2806, 2820
AccB1I	GGYRCC	3 cut(s)	320, 350, 715
AccB7I	CCANNNNNTGG	1 cut(s)	2450
AccBSI	CCGCTC	2 cut(s)	147, 235
AccII	CGCG	3 cut(s)	381, 400, 596
AccIII	TCCGGA	2 cut(s)	472, 2143
AclWI	GGATC	6 cut(s)	102, 115, 530, 1163, 1799, 3004
AcsI	RAATTY	2 cut(s)	461, 2413
AcuI	CTGAAG	2 cut(s)	1008, 1539
AcyI	GRCGYC	2 cut(s)	351, 716
AfaI	GTAC	5 cut(s)	362, 927, 1119, 2034, 2938
AfiI	CCNNNNNNNGG	9 cut(s)	184, 317, 409, 1491, 1492, 1741, 1796, 2450, 2735
AflIII	ACRYGT	1 cut(s)	1264
AgsI	TTSAA	6 cut(s)	1373, 1671, 1778, 1918, 2325, 2962
AhdI	GACNNNNNGTC	1 cut(s)	1429
AjnI	CCWGG	1 cut(s)	365
AjuI	GAANNNNNNNTTGG	4 cut(s)	1521, 1553, 2437, 2469
AleI	CACNNNNGTG	1 cut(s)	432
Alw21I	GWGCWC	6 cut(s)	33, 1042, 1872, 2062, 2680, 2882
Alw26I	GTCTC	5 cut(s)	258, 899, 1416, 1586, 2699
AlwI	GGATC	6 cut(s)	102, 115, 530, 1163, 1799, 3004
AlwNI	CAGNNNCTG	1 cut(s)	941
Ama87I	CYCGRG	3 cut(s)	200, 245, 261
Aor13HI	TCCGGA	2 cut(s)	472, 2143
AoxI	GGCC	6 cut(s)	76, 304, 342, 741, 1560, 1941
ApoI	RAATTY	2 cut(s)	461, 2413
ArsI	GACNNNNNNTTYG	2 cut(s)	820, 852
Asp700I	GAANNNNTTC	1 cut(s)	461
AspA2I	CCTAGG	1 cut(s)	2699
AspLEI	GCGC	9 cut(s)	14, 207, 353, 402, 598, 718, 796, 919, 2311
AspS9I	GGNCC	6 cut(s)	77, 250, 667, 783, 1732, 2738
AsuHPI	GGTGA	7 cut(s)	328, 466, 493, 956, 1034, 2415, 2612
AsuII	TTCGAA	1 cut(s)	826
AvaI	CYCGRG	3 cut(s)	200, 245, 261
AvaII	GGWCC	5 cut(s)	250, 667, 783, 1732, 2738
AvrII	CCTAGG	1 cut(s)	2699
AxyI	CCTNAGG	2 cut(s)	1740, 3005
BamHI	GGATCC	1 cut(s)	107
BanI	GGYRCC	3 cut(s)	320, 350, 715
BanII	GRGCYC	4 cut(s)	33, 192, 1872, 2680
BbsI	GAAGAC	4 cut(s)	833, 1545, 2156, 2313
Bbv12I	GWGCWC	6 cut(s)	33, 1042, 1872, 2062, 2680, 2882
BbvCI	CCTCAGC	1 cut(s)	1386
BccI	CCATC	6 cut(s)	71, 332, 1053, 1177, 2263, 2561
BceAI	ACGGC	5 cut(s)	91, 314, 319, 586, 2969
BciT130I	CCWGG	1 cut(s)	367
BciVI	GTATCC	3 cut(s)	1140, 1507, 2934
BcoDI	GTCTC	5 cut(s)	258, 899, 1416, 1586, 2699
BfaI	CTAG	2 cut(s)	1491, 2700
BfmI	CTRYAG	4 cut(s)	1194, 1997, 2714, 2813
BfoI	RGCGCY	4 cut(s)	15, 354, 719, 797
BfuAI	ACCTGC	2 cut(s)	2806, 2820
BfuI	GTATCC	3 cut(s)	1140, 1507, 2934
BglII	AGATCT	1 cut(s)	2327
BlnI	CCTAGG	1 cut(s)	2699
BmcAI	AGTACT	1 cut(s)	2034
Bme1390I	CCNGG	1 cut(s)	367
Bme18I	GGWCC	5 cut(s)	250, 667, 783, 1732, 2738
BmeRI	GACNNNNNGTC	1 cut(s)	1429
BmeT110I	CYCGRG	3 cut(s)	200, 245, 261
BmgT120I	GGNCC	6 cut(s)	77, 250, 667, 783, 1732, 2738
BmrFI	CCNGG	1 cut(s)	367
BmrI	ACTGGG	1 cut(s)	166
BmsI	GCATC	9 cut(s)	947, 1028, 1063, 1402, 1821, 2164, 2320, 2563, 2569
BmuI	ACTGGG	1 cut(s)	166
BpiI	GAAGAC	4 cut(s)	833, 1545, 2156, 2313
BplI	GAGNNNNNCTC	6 cut(s)	20, 52, 179, 211, 255, 287
BpmI	CTGGAG	1 cut(s)	1458
Bpu10I	CCTNAGC	2 cut(s)	81, 1386
Bpu14I	TTCGAA	1 cut(s)	826
BpuEI	CTTGAG	5 cut(s)	1198, 1316, 1769, 1862, 2664
BsaHI	GRCGYC	2 cut(s)	351, 716
BsaI	GGTCTC	3 cut(s)	899, 1416, 1586
BsaWI	WCCGGW	2 cut(s)	472, 2143
BsaXI	ACNNNNNCTCC	2 cut(s)	152, 182
Bsc4I	CCNNNNNNNGG	9 cut(s)	184, 317, 409, 1491, 1492, 1741, 1796, 2450, 2735
Bse118I	RCCGGY	1 cut(s)	215
Bse1I	ACTGG	6 cut(s)	172, 562, 1475, 1515, 1549, 2045
Bse21I	CCTNAGG	2 cut(s)	1740, 3005
BseAI	TCCGGA	2 cut(s)	472, 2143
BseBI	CCWGG	1 cut(s)	367
BseGI	GGATG	6 cut(s)	68, 267, 481, 1078, 1417, 1978
BseLI	CCNNNNNNNGG	9 cut(s)	184, 317, 409, 1491, 1492, 1741, 1796, 2450, 2735
BseMII	CTCAG	5 cut(s)	652, 1377, 1731, 2001, 2256
BseNI	ACTGG	6 cut(s)	172, 562, 1475, 1515, 1549, 2045
BseRI	GAGGAG	8 cut(s)	77, 80, 227, 263, 1295, 1514, 1625, 2090
BseYI	CCCAGC	1 cut(s)	1891
BsgI	GTGCAG	2 cut(s)	1308, 2214
Bsh1236I	CGCG	3 cut(s)	381, 400, 596
Bsh1285I	CGRYCG	1 cut(s)	376
BshFI	GGCC	6 cut(s)	78, 306, 344, 743, 1562, 1943
BshNI	GGYRCC	3 cut(s)	320, 350, 715
BsiEI	CGRYCG	1 cut(s)	376
BsiHKAI	GWGCWC	6 cut(s)	33, 1042, 1872, 2062, 2680, 2882
BsiHKCI	CYCGRG	3 cut(s)	200, 245, 261
BsiSI	CCGG	7 cut(s)	138, 216, 424, 473, 1763, 2144, 2168
BslFI	GGGAC	9 cut(s)	160, 383, 769, 890, 1076, 1405, 2221, 2675, 2751
BslI	CCNNNNNNNGG	9 cut(s)	184, 317, 409, 1491, 1492, 1741, 1796, 2450, 2735
BsmAI	GTCTC	5 cut(s)	258, 899, 1416, 1586, 2699
BsmFI	GGGAC	9 cut(s)	160, 383, 769, 890, 1076, 1405, 2221, 2675, 2751
BsnI	GGCC	6 cut(s)	78, 306, 344, 743, 1562, 1943
Bso31I	GGTCTC	3 cut(s)	899, 1416, 1586
BsoBI	CYCGRG	3 cut(s)	200, 245, 261
Bsp119I	TTCGAA	1 cut(s)	826
Bsp1286I	GDGCHC	7 cut(s)	33, 192, 1042, 1872, 2062, 2680, 2882
Bsp13I	TCCGGA	2 cut(s)	472, 2143
Bsp1407I	TGTACA	1 cut(s)	925
Bsp143I	GATC	9 cut(s)	54, 107, 522, 1168, 1804, 2287, 2327, 2859, 2996
Bsp19I	CCATGG	2 cut(s)	1077, 1158
BspANI	GGCC	6 cut(s)	78, 306, 344, 743, 1562, 1943
BspCNI	CTCAG	5 cut(s)	651, 1378, 1732, 2000, 2257
BspEI	TCCGGA	2 cut(s)	472, 2143
BspFNI	CGCG	3 cut(s)	381, 400, 596
BspHI	TCATGA	2 cut(s)	688, 1443
BspMAI	CTGCAG	2 cut(s)	2001, 2817
BspMI	ACCTGC	2 cut(s)	2806, 2820
BspPI	GGATC	6 cut(s)	102, 115, 530, 1163, 1799, 3004
BspT104I	TTCGAA	1 cut(s)	826
BspT107I	GGYRCC	3 cut(s)	320, 350, 715
BspTNI	GGTCTC	3 cut(s)	899, 1416, 1586
BsrBI	CCGCTC	2 cut(s)	147, 235
BsrFI	RCCGGY	1 cut(s)	215
BsrGI	TGTACA	1 cut(s)	925
BsrI	ACTGG	6 cut(s)	172, 562, 1475, 1515, 1549, 2045
BssAI	RCCGGY	1 cut(s)	215
BssMI	GATC	9 cut(s)	54, 107, 522, 1168, 1804, 2287, 2327, 2859, 2996
BssNI	GRCGYC	2 cut(s)	351, 716
BssT1I	CCWWGG	6 cut(s)	339, 345, 1077, 1158, 1563, 2699
Bst2UI	CCWGG	1 cut(s)	367
Bst6I	CTCTTC	5 cut(s)	621, 902, 1392, 1707, 2372
BstACI	GRCGYC	2 cut(s)	351, 716
BstAUI	TGTACA	1 cut(s)	925
BstBI	TTCGAA	1 cut(s)	826
BstC8I	GCNNGC	8 cut(s)	217, 594, 684, 997, 1926, 1941, 1965, 2229
BstDEI	CTNAG	9 cut(s)	81, 638, 1386, 1740, 1951, 1987, 2265, 2493, 3005
BstDSI	CCRYGG	4 cut(s)	379, 1077, 1158, 2985
BstENI	CCTNNNNNAGG	1 cut(s)	182
BstF5I	GGATG	6 cut(s)	68, 267, 481, 1078, 1417, 1978
BstFNI	CGCG	3 cut(s)	381, 400, 596
BstH2I	RGCGCY	4 cut(s)	15, 354, 719, 797
BstHHI	GCGC	9 cut(s)	14, 207, 353, 402, 598, 718, 796, 919, 2311
BstKTI	GATC	9 cut(s)	57, 110, 525, 1171, 1807, 2290, 2330, 2862, 2999
BstMAI	GTCTC	5 cut(s)	258, 899, 1416, 1586, 2699
BstMBI	GATC	9 cut(s)	54, 107, 522, 1168, 1804, 2287, 2327, 2859, 2996
BstMCI	CGRYCG	1 cut(s)	376
BstMWI	GCNNNNNNNGC	8 cut(s)	350, 390, 399, 598, 869, 982, 1016, 2886
BstNI	CCWGG	1 cut(s)	367
BstNSI	RCATGY	3 cut(s)	1268, 1928, 1939
BstSCI	CCNGG	1 cut(s)	365
BstSFI	CTRYAG	4 cut(s)	1194, 1997, 2714, 2813
BstUI	CGCG	3 cut(s)	381, 400, 596
BstV2I	GAAGAC	4 cut(s)	833, 1545, 2156, 2313
BstX2I	RGATCY	3 cut(s)	107, 2327, 2996
BstYI	RGATCY	3 cut(s)	107, 2327, 2996
Bsu36I	CCTNAGG	2 cut(s)	1740, 3005
BsuI	GTATCC	3 cut(s)	1140, 1507, 2934
BsuRI	GGCC	6 cut(s)	78, 306, 344, 743, 1562, 1943
BtgI	CCRYGG	4 cut(s)	379, 1077, 1158, 2985
BtgZI	GCGATG	1 cut(s)	310
BtsCI	GGATG	6 cut(s)	68, 267, 481, 1078, 1417, 1978
BtsIMutI	CAGTG	6 cut(s)	1563, 1800, 2273, 2547, 2787, 2878
BveI	ACCTGC	2 cut(s)	2806, 2820
Cac8I	GCNNGC	8 cut(s)	217, 594, 684, 997, 1926, 1941, 1965, 2229
CaiI	CAGNNNCTG	1 cut(s)	941
CciI	TCATGA	2 cut(s)	688, 1443
CfoI	GCGC	9 cut(s)	14, 207, 353, 402, 598, 718, 796, 919, 2311
Cfr10I	RCCGGY	1 cut(s)	215
Cfr13I	GGNCC	6 cut(s)	77, 250, 667, 783, 1732, 2738
Cfr42I	CCGCGG	1 cut(s)	382
CseI	GACGC	1 cut(s)	2594
Csp6I	GTAC	5 cut(s)	361, 926, 1118, 2033, 2937
CspCI	CAANNNNNGTGG	4 cut(s)	1444, 1479, 2496, 2531
CviQI	GTAC	5 cut(s)	361, 926, 1118, 2033, 2937
DdeI	CTNAG	9 cut(s)	81, 638, 1386, 1740, 1951, 1987, 2265, 2493, 3005
DinI	GGCGCC	2 cut(s)	352, 717
DpnI	GATC	9 cut(s)	56, 109, 524, 1170, 1806, 2289, 2329, 2861, 2998
DpnII	GATC	9 cut(s)	54, 107, 522, 1168, 1804, 2287, 2327, 2859, 2996
DriI	GACNNNNNGTC	1 cut(s)	1429
Eam1104I	CTCTTC	5 cut(s)	621, 902, 1392, 1707, 2372
Eam1105I	GACNNNNNGTC	1 cut(s)	1429
EarI	CTCTTC	5 cut(s)	621, 902, 1392, 1707, 2372
EciI	GGCGGA	2 cut(s)	972, 2419
Ecl136II	GAGCTC	3 cut(s)	31, 1870, 2678
Eco130I	CCWWGG	6 cut(s)	339, 345, 1077, 1158, 1563, 2699
Eco147I	AGGCCT	1 cut(s)	743
Eco24I	GRGCYC	4 cut(s)	33, 192, 1872, 2680
Eco31I	GGTCTC	3 cut(s)	899, 1416, 1586
Eco32I	GATATC	1 cut(s)	294
Eco47I	GGWCC	5 cut(s)	250, 667, 783, 1732, 2738
Eco53kI	GAGCTC	3 cut(s)	31, 1870, 2678
Eco57I	CTGAAG	2 cut(s)	1008, 1539
Eco81I	CCTNAGG	2 cut(s)	1740, 3005
Eco88I	CYCGRG	3 cut(s)	200, 245, 261
EcoICRI	GAGCTC	3 cut(s)	31, 1870, 2678
EcoNI	CCTNNNNNAGG	1 cut(s)	182
EcoO109I	RGGNCCY	2 cut(s)	250, 2738
EcoRI	GAATTC	1 cut(s)	461
EcoRII	CCWGG	1 cut(s)	365
EcoRV	GATATC	1 cut(s)	294
EcoT14I	CCWWGG	6 cut(s)	339, 345, 1077, 1158, 1563, 2699
EcoT38I	GRGCYC	4 cut(s)	33, 192, 1872, 2680
EgeI	GGCGCC	2 cut(s)	352, 717
EheI	GGCGCC	2 cut(s)	352, 717
ErhI	CCWWGG	6 cut(s)	339, 345, 1077, 1158, 1563, 2699
FalI	AAGNNNNNCTT	4 cut(s)	1521, 1553, 2749, 2781
FaqI	GGGAC	9 cut(s)	160, 383, 769, 890, 1076, 1405, 2221, 2675, 2751
FauI	CCCGC	1 cut(s)	152
FokI	GGATG	6 cut(s)	55, 254, 488, 1085, 1424, 1985
FriOI	GRGCYC	4 cut(s)	33, 192, 1872, 2680
FspBI	CTAG	2 cut(s)	1491, 2700
FspI	TGCGCA	1 cut(s)	918
GlaI	GCGC	9 cut(s)	13, 206, 352, 401, 597, 717, 795, 918, 2310
GsaI	CCCAGC	1 cut(s)	1895
GsuI	CTGGAG	1 cut(s)	1458
HaeII	RGCGCY	4 cut(s)	15, 354, 719, 797
HaeIII	GGCC	6 cut(s)	78, 306, 344, 743, 1562, 1943
HapII	CCGG	7 cut(s)	138, 216, 424, 473, 1763, 2144, 2168
HgaI	GACGC	1 cut(s)	2594
HhaI	GCGC	9 cut(s)	14, 207, 353, 402, 598, 718, 796, 919, 2311
Hin1I	GRCGYC	2 cut(s)	351, 716
Hin6I	GCGC	9 cut(s)	12, 205, 351, 400, 596, 716, 794, 917, 2309
HinP1I	GCGC	9 cut(s)	12, 205, 351, 400, 596, 716, 794, 917, 2309
HincII	GTYRAC	1 cut(s)	2616
HindII	GTYRAC	1 cut(s)	2616
HindIII	AAGCTT	4 cut(s)	680, 957, 2252, 2763
HpaII	CCGG	7 cut(s)	138, 216, 424, 473, 1763, 2144, 2168
HphI	GGTGA	7 cut(s)	328, 466, 493, 956, 1034, 2415, 2612
Hpy166II	GTNNAC	6 cut(s)	7, 361, 667, 1906, 2248, 2616
Hpy8I	GTNNAC	6 cut(s)	7, 361, 667, 1906, 2248, 2616
Hpy99I	CGWCG	4 cut(s)	29, 107, 434, 840
HpyAV	CCTTC	8 cut(s)	403, 451, 591, 630, 686, 733, 1143, 2600
HpyCH4IV	ACGT	2 cut(s)	2161, 2191
HpyF10VI	GCNNNNNNNGC	8 cut(s)	350, 390, 399, 598, 869, 982, 1016, 2886
HpyF3I	CTNAG	9 cut(s)	81, 638, 1386, 1740, 1951, 1987, 2265, 2493, 3005
HpySE526I	ACGT	2 cut(s)	2161, 2191
Hsp92I	GRCGYC	2 cut(s)	351, 716
HspAI	GCGC	9 cut(s)	12, 205, 351, 400, 596, 716, 794, 917, 2309
KasI	GGCGCC	2 cut(s)	350, 715
KflI	GGGWCCC	1 cut(s)	2738
Kpn2I	TCCGGA	2 cut(s)	472, 2143
KroI	GCCGGC	1 cut(s)	215
KroNI	GCCGGC	1 cut(s)	217
KspI	CCGCGG	1 cut(s)	382
Kzo9I	GATC	9 cut(s)	54, 107, 522, 1168, 1804, 2287, 2327, 2859, 2996
LweI	GCATC	9 cut(s)	947, 1028, 1063, 1402, 1821, 2164, 2320, 2563, 2569
MaeI	CTAG	2 cut(s)	1491, 2700
MaeII	ACGT	2 cut(s)	2161, 2191
MaeIII	GTNAC	1 cut(s)	1477
MalI	GATC	9 cut(s)	56, 109, 524, 1170, 1806, 2289, 2329, 2861, 2998
MbiI	CCGCTC	2 cut(s)	147, 235
MboI	GATC	9 cut(s)	54, 107, 522, 1168, 1804, 2287, 2327, 2859, 2996
MfeI	CAATTG	2 cut(s)	1770, 1863
MflI	RGATCY	3 cut(s)	107, 2327, 2996
MhlI	GDGCHC	7 cut(s)	33, 192, 1042, 1872, 2062, 2680, 2882
Mly113I	GGCGCC	2 cut(s)	351, 716
MlyI	GAGTC	7 cut(s)	1193, 1307, 1439, 1724, 1775, 2294, 2960
MmeI	TCCRAC	6 cut(s)	63, 126, 183, 686, 1399, 1759
MroI	TCCGGA	2 cut(s)	472, 2143
MroNI	GCCGGC	1 cut(s)	215
MroXI	GAANNNNTTC	1 cut(s)	461
MseI	TTAA	7 cut(s)	932, 1338, 2054, 2370, 2469, 2795, 2820
MslI	CAYNNNNRTG	2 cut(s)	432, 1325
MspA1I	CMGCKG	1 cut(s)	381
MspI	CCGG	7 cut(s)	138, 216, 424, 473, 1763, 2144, 2168
MspR9I	CCNGG	1 cut(s)	367
MunI	CAATTG	2 cut(s)	1770, 1863
MvaI	CCWGG	1 cut(s)	367
MvnI	CGCG	3 cut(s)	381, 400, 596
MwoI	GCNNNNNNNGC	8 cut(s)	350, 390, 399, 598, 869, 982, 1016, 2886
NaeI	GCCGGC	1 cut(s)	217
NarI	GGCGCC	2 cut(s)	351, 716
NcoI	CCATGG	2 cut(s)	1077, 1158
NdeII	GATC	9 cut(s)	54, 107, 522, 1168, 1804, 2287, 2327, 2859, 2996
NgoMIV	GCCGGC	1 cut(s)	215
NmeAIII	GCCGAG	2 cut(s)	72, 297
NsbI	TGCGCA	1 cut(s)	918
NspI	RCATGY	3 cut(s)	1268, 1928, 1939
NspV	TTCGAA	1 cut(s)	826
OliI	CACNNNNGTG	1 cut(s)	432
PaeI	GCATGC	1 cut(s)	1928
PaeR7I	CTCGAG	2 cut(s)	200, 245
PagI	TCATGA	2 cut(s)	688, 1443
PceI	AGGCCT	1 cut(s)	743
PciI	ACATGT	1 cut(s)	1264
PcsI	WCGNNNNNNNCGW	4 cut(s)	24, 99, 787, 832
PdiI	GCCGGC	1 cut(s)	217
PdmI	GAANNNNTTC	1 cut(s)	461
PflMI	CCANNNNNTGG	1 cut(s)	2450
PleI	GAGTC	7 cut(s)	1193, 1306, 1438, 1723, 1774, 2294, 2960
PluTI	GGCGCC	2 cut(s)	354, 719
PpsI	GAGTC	7 cut(s)	1193, 1306, 1438, 1723, 1774, 2294, 2960
PpuMI	RGGWCCY	2 cut(s)	250, 2738
PscI	ACATGT	1 cut(s)	1264
Psp124BI	GAGCTC	3 cut(s)	33, 1872, 2680
Psp5II	RGGWCCY	2 cut(s)	250, 2738
Psp6I	CCWGG	1 cut(s)	365
PspFI	CCCAGC	1 cut(s)	1891
PspGI	CCWGG	1 cut(s)	365
PspPI	GGNCC	6 cut(s)	77, 250, 667, 783, 1732, 2738
PspPPI	RGGWCCY	2 cut(s)	250, 2738
PspXI	VCTCGAGB	1 cut(s)	200
PstI	CTGCAG	2 cut(s)	2001, 2817
PstNI	CAGNNNCTG	1 cut(s)	941
PsuI	RGATCY	3 cut(s)	107, 2327, 2996
RsaI	GTAC	5 cut(s)	362, 927, 1119, 2034, 2938
RsaNI	GTAC	5 cut(s)	361, 926, 1118, 2033, 2937
RseI	CAYNNNNRTG	2 cut(s)	432, 1325
SacI	GAGCTC	3 cut(s)	33, 1872, 2680
SacII	CCGCGG	1 cut(s)	382
SaqAI	TTAA	7 cut(s)	932, 1338, 2054, 2370, 2469, 2795, 2820
Sau3AI	GATC	9 cut(s)	54, 107, 522, 1168, 1804, 2287, 2327, 2859, 2996
Sau96I	GGNCC	6 cut(s)	77, 250, 667, 783, 1732, 2738
SbfI	CCTGCAGG	1 cut(s)	2817
ScaI	AGTACT	1 cut(s)	2034
SchI	GAGTC	7 cut(s)	1193, 1307, 1439, 1724, 1775, 2294, 2960
ScrFI	CCNGG	1 cut(s)	367
SdaI	CCTGCAGG	1 cut(s)	2817
SduI	GDGCHC	7 cut(s)	33, 192, 1042, 1872, 2062, 2680, 2882
SfaNI	GCATC	9 cut(s)	947, 1028, 1063, 1402, 1821, 2164, 2320, 2563, 2569
SfcI	CTRYAG	4 cut(s)	1194, 1997, 2714, 2813
SfoI	GGCGCC	2 cut(s)	352, 717
Sfr274I	CTCGAG	2 cut(s)	200, 245
Sfr303I	CCGCGG	1 cut(s)	382
SfuI	TTCGAA	1 cut(s)	826
SgrBI	CCGCGG	1 cut(s)	382
SinI	GGWCC	5 cut(s)	250, 667, 783, 1732, 2738
SlaI	CTCGAG	2 cut(s)	200, 245
SmiMI	CAYNNNNRTG	2 cut(s)	432, 1325
SmlI	CTYRAG	7 cut(s)	200, 245, 1213, 1295, 1784, 1841, 2679
SmoI	CTYRAG	7 cut(s)	200, 245, 1213, 1295, 1784, 1841, 2679
SphI	GCATGC	1 cut(s)	1928
Sse8387I	CCTGCAGG	1 cut(s)	2817
SseBI	AGGCCT	1 cut(s)	743
SspDI	GGCGCC	2 cut(s)	350, 715
SspMI	CTAG	2 cut(s)	1491, 2700
SstI	GAGCTC	3 cut(s)	33, 1872, 2680
StuI	AGGCCT	1 cut(s)	743
StyD4I	CCNGG	1 cut(s)	365
StyI	CCWWGG	6 cut(s)	339, 345, 1077, 1158, 1563, 2699
TaiI	ACGT	2 cut(s)	2164, 2194
TaqI	TCGA	8 cut(s)	27, 57, 201, 246, 764, 826, 1829, 2290
TaqII	GACCGA	1 cut(s)	771
TatI	WGTACW	5 cut(s)	360, 925, 1117, 2032, 2936
TauI	GCSGC	5 cut(s)	235, 381, 384, 601, 1010
Tru1I	TTAA	7 cut(s)	932, 1338, 2054, 2370, 2469, 2795, 2820
Tru9I	TTAA	7 cut(s)	932, 1338, 2054, 2370, 2469, 2795, 2820
TscAI	CASTG	6 cut(s)	1563, 1807, 2280, 2554, 2787, 2878
TspGWI	ACGGA	5 cut(s)	13, 120, 725, 792, 2718
TspRI	CASTG	6 cut(s)	1563, 1807, 2280, 2554, 2787, 2878
Van91I	CCANNNNNTGG	1 cut(s)	2450
VpaK11BI	GGWCC	5 cut(s)	250, 667, 783, 1732, 2738
XagI	CCTNNNNNAGG	1 cut(s)	182
XapI	RAATTY	2 cut(s)	461, 2413
XceI	RCATGY	3 cut(s)	1268, 1928, 1939
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1156, 1816
XhoI	CTCGAG	2 cut(s)	200, 245
XmaJI	CCTAGG	1 cut(s)	2699
XmnI	GAANNNNTTC	1 cut(s)	461
XspI	CTAG	2 cut(s)	1491, 2700
ZrmI	AGTACT	1 cut(s)	2034

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.