Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0009569

Physical Location & Seq

Genomic Coordinates Reverse (-)

12171131 .. 12177743

Gene Structure 6613 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1147600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3015 bp

ATGGTACACAGTGCCTATGATTCCTTCGAGCTCATCAGCGACTGCCCGACGAAGATCGAAGCCATCGAATCGTACGGACAGAAGCTCCTCATCGGCTGCTCCGATGGATCTCTCAAAATCTACGCTCCGGACTCCTCCGGCTCCGACCGCTCTCCGCCGTCCGATTACCACGGTCACAAGCTCCAGAAGGAACCGTACGCGCTGGAGAGGAACGTCGCGGGTTTCTCAAAGAAGCCCTTGGTTTCAATGGAGGTCCTGGAATCGCGAGAGCTCCTCCTTTCCCTCTCGGAGTCGATTGCGTTTCACGGCCTCCCGAACTTGGGCACCATCGCCGTGATCACGAAGGCGAAGGGCGCCAATGTCTACTCGTGGGACGACCGCAGAGGGTTCCTTTGCTTCGCCAGGCAGAAGCGGGTCTGTATTTTCCGGCACGACGGGGGACGAGGGTTTGTGGAAGTGAAGGAATTTGGGGTTCCGGATGTGGTGAAGTCGATGTCATGGTGTGGAGAGAACATATGTATAGGGATTAGAAGAGAGTATATGATACTGAATTCTACAAATGGTGCACTGTCAGAGGTGTTTCCTTCGGGGAGGATAGCGCCGCCATTGGTGGTCTCTCTCCCTTCCGGAGAGCTTCTTCTGGGAAAGGATAATATTGGGGTTTTTGTGGACCAAAACGGGAAGCTTTGTCAAGAAGGCAGGGTTTGTTGGTCAGATTCCCCGAATGTTGTTGTTGTTCAGAAGCCATATGCAATTGCTCTGTTACCAAGATATGTGGAGGTACGGTCGCTCCGAGATCCATATCCATTGATACAGACGGTAGTTCTTAGAAATGCTCGTCGTATTCTACAGAGCAATGATGCTGTAACTGTTGCATTAGAGAATGCTGTTTATGGTCTCTTCCCTGTTCCTCTTGGTGCTCAGATTGTACAATTAACAGCATCTGGCGATTTTGACGAAGCATTGGCATTGTGTAAGATGCTTCCTCCAGAAGAAGCAAGTCTTAGAGCTGCAAAGGAGGGGTCAATTCATATGAGATATGCTCATCATCTTTTTGATAATGGGGCCTATGAGGACGCCATGGAGCACTTTTTGGCATCTCAAGTGGATATAACCTATGTTCTTTCTTTGTATCCATCTATTATTCTTCCTAAGACAACTATGGTTGCTGATCCGGAGAAGCTAATGGATATTTCTGGGGATTCATTGTATCTCTCAAGAGGTTCGTCTGGTATATCAGATGATATGGAACACTCTCTGCCTTCACTACTGGAATCTGAGGAGAGTGCAGCACTCGAGTCCAAGAAAATGAGCCATAACACTCTTATGGCTCTCATAAAGTTCTTACAGAAAAAAAGATATGGCATAATTGAAAAGGCCACTGCTGAGGGGACAGAAGAGGTTGTTTTAGATGCTGTTGGAGACAATTTTGTGTCCTATGAATCTAGGTTTAAGAAATCAAACAAGGGGCGTGGCAGCATTCCAGTTACCTCTGGTGCTAGGGAGATGGCAGCGATCCTGGATACTGCACTACTCCAAGCTCTACTTCTTACTGGACAGGCTTCAGTGGCTTTGGAATTACTGAAAGGTCTTAACTACTGCGATGTAAAAATATGTGAGGAGATTCTCCAGAAAAATAATCACTACGCTGCTTTGTTAGAACTTTACAGGTGCAATTCAATGCACCACGAAGCTCTAAAACTCTTGCATCAATTAGTAGAGGATTCTAAATCTAACCAAGTGCAAACTGAACTTATCCAAAAGTTAAAGCCTGAGTCAATTGTTGAATATCTCAAGCCTCTTTGTGGGACTGATCCCATGCTGGTCCTGGAGTATTCAATGCTTGTTCTTGAAAGTTGTCCAACGCAGACAATTGAGCTCTTTTTGAATGGGAATATTCCAGCAGACCTGGTCAACTCTTACTTGAAGCAACATGCTCCAAACATGCAGGCCACATACTTAGAGTTTATGCTTGCAATGGATGAAAATGGAATCTCTGGAAATCTGCAAAATGAAATGGTACATATCTATCTATCAGAAGTACTTGATTGGTATGCAGATTTAAGTACTCAGCAGAAATGGGATGAGCATACTTACTCCTCAACAAGAAAGAAATTATTGTCTGCTTTAGAGAGTATCTCAGGATATAATCCGGAGGCTTTGTTGAAACGTCTTCCAACGGATGCTTTATATGAAGAGCGTGCAATTTTGTTGGGAAAAATGAACCAACATGAGCTTGCTTTATCTCTCTATGTTCATAAGCTTCATGTTCCTGAGCTGGCACTGTCCCATTGTGATCGCGTGTATGAATCCTTGGTTCATCTACCATCTTCAAGATCTTCCGGAAATATATACCTAACTCTATTACAAATTTATCTTAATCCGAGGAAAACAACCAAGAACTTTGAGAAGCGAATTACAAATTTAGTATCACCTCAGAATATCGGTACACCAAAGGTTGGTTCTGCAAATATGGTTAAAAGTAAAGGAGGGCGTGGTGCTAAAAAAATTGCTGCAATAGAGGTGGCACCTGACATAAGAGTCAGTCAAAGTAGCGCTGACAGTAGCAAGAGTGATGGAGATGCAGAGGAATCTAGTGAGGAAGGAGGGTCGAAAATCATGCTTGATGAGGTCCTTGATTTGTTGAGCCGAAGGTGGGACAGAATAAATGGAGCTCAGGCACTCAAACTTTTGCCAAGGGAAACTAAGCTACAGCACTTGCTTCCATTTATGGGACCCCTGTTGAGGAAATCCAGTGAAGCGTACAGGAACCTTTCAGTTATCAAGAGTTTGAGACAAAGCGAGAACCTGCAGGTTAAAGATGAACTCTACGAACAAAGGAAGGGTGTGGTGAAGGTCACCAGTGACAGCATGTGCTCCCTTTGCAGGAAGAAAATAGGAACCAGTGTCTTTGCCGTATATCCAAACGGGAAAACATTAGTGCACTTTGTGTGCTTTAGAGACTCGCAAAGTATGAAAACCGTGGGCAGAGGATCGCCGCTATGGAAGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0000325 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005770 GO:0005773 GO:0005774 GO:0005911 GO:0006810 GO:0006906 GO:0006914 GO:0006996 GO:0007033 GO:0007034 GO:0007041 GO:0008150 GO:0008152 GO:0008333 GO:0009056 GO:0009506 GO:0009705 GO:0009987 GO:0010008 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016197 GO:0030054 GO:0030897 GO:0031090 GO:0031410 GO:0031902 GO:0031982 GO:0032991 GO:0034058 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044248 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048284 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055044 GO:0061024 GO:0061025 GO:0061919 GO:0071840 GO:0090174 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:0098876 GO:0099023 GO:1902774 GO:1990126

KEGG Pathways

Metabolic & Signaling

ko04138 map04138

Pfam Domains

Protein Families

CNH Clathrin Vps39_1 Vps39_2

Protein Analysis

1005

Amino Acids

111.49

Weight (kDa)

6.37

Isoelectric Point (pI)

45.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CNH	PF00780	26 - 280	1.5e-21	CNH domain
Clathrin	PF00637	504 - 598	1.2e-08	Region in Clathrin and VPS
Vps39_1	PF10366	507 - 617	1.8e-25	Vacuolar sorting protein 39 domain 1
zf-Vps39_C	PF10367	950 - 988	1.6e-17	Vacuolar sorting protein 39 C-terminal zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009569)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36630 AT4G36630
fragaria_vesca	FvH4_1g13311
malus_domestica	MD02G1147600.v1.1 MD15G1261900.v1.1
prunus_persica	Prupe.7G154500_v2.0.a1
pyrus_communis	pycom02g11660 pycom15g22910
rosa_chinensis	RchiOBHm_Chr2g0101111 RchiOBHm_Chr2g0101581
rosa_laevigata	RLG00000017092
rosa_multiflora	Rmu_sc0006153.1_g000006 Rmu_sc0019613.1_g000003
rosa_roxburghii	Rroxscaffold_2G00141170
rosa_rugosa	Rorug02G0097300 Rorug02G0100900
rosa_samantha	Rh2AG148200 Rh2BG153600 Rh2CG149800 Rh2CG153900 Rh2DG153900
rosa_wichuraiana	Rw2G011560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2540
Acc36I	ACCTGC	2 cut(s)	2803, 2817
AccB1I	GGYRCC	3 cut(s)	323, 353, 2527
AccB7I	CCANNNNNTGG	1 cut(s)	2459
AccBSI	CCGCTC	1 cut(s)	150
AccI	GTMKAC	1 cut(s)	363
AccII	CGCG	4 cut(s)	200, 218, 265, 2301
AccIII	TCCGGA	6 cut(s)	127, 475, 626, 1174, 2152, 2342
AciI	CCGC	7 cut(s)	148, 155, 218, 379, 412, 602, 2999
AclWI	GGATC	6 cut(s)	115, 791, 1166, 1510, 1808, 3001
AcsI	RAATTY	4 cut(s)	464, 550, 2370, 2422
AcuI	CTGAAG	1 cut(s)	1548
AcyI	GRCGYC	2 cut(s)	354, 1077
AdeI	CACNNNGTG	1 cut(s)	2950
AfeI	AGCGCT	1 cut(s)	2557
AfiI	CCNNNNNNNGG	8 cut(s)	319, 320, 1805, 2459, 2656, 2732, 2745, 2886
AjnI	CCWGG	5 cut(s)	255, 401, 1518, 1827, 1908
AjuI	GAANNNNNNNTTGG	4 cut(s)	1530, 1562, 2446, 2478
Alw21I	GWGCWC	9 cut(s)	33, 273, 568, 922, 1089, 1881, 2677, 2879, 2946
Alw26I	GTCTC	5 cut(s)	619, 902, 1416, 2788, 2955
Alw44I	GTGCAC	2 cut(s)	564, 2942
AlwI	GGATC	6 cut(s)	115, 791, 1166, 1510, 1808, 3001
AlwNI	CAGNNNCTG	2 cut(s)	42, 944
Ama87I	CYCGRG	1 cut(s)	1295
Aor13HI	TCCGGA	6 cut(s)	127, 475, 626, 1174, 2152, 2342
Aor51HI	AGCGCT	1 cut(s)	2557
AoxI	GGCC	4 cut(s)	307, 1065, 1377, 1950
ApaLI	GTGCAC	2 cut(s)	564, 2942
ApeKI	GCWGC	7 cut(s)	96, 1010, 1289, 1476, 1511, 1649, 2513
ApoI	RAATTY	4 cut(s)	464, 550, 2370, 2422
ArsI	GACNNNNNNTTYG	2 cut(s)	1007, 1039
AspLEI	GCGC	4 cut(s)	202, 356, 601, 2558
AspS9I	GGNCC	6 cut(s)	253, 670, 1065, 1825, 2632, 2735
AsuHPI	GGTGA	4 cut(s)	496, 2424, 2851, 2863
AvaI	CYCGRG	1 cut(s)	1295
AvaII	GGWCC	5 cut(s)	253, 670, 1825, 2632, 2735
BaeGI	GKGCMC	3 cut(s)	326, 568, 2946
BanI	GGYRCC	3 cut(s)	323, 353, 2527
BanII	GRGCYC	4 cut(s)	33, 273, 1881, 2677
BarI	GAAGNNNNNNTAC	2 cut(s)	179, 211
BauI	CACGAG	1 cut(s)	367
BbsI	GAAGAC	1 cut(s)	2165
Bbv12I	GWGCWC	9 cut(s)	33, 273, 568, 922, 1089, 1881, 2677, 2879, 2946
BbvCI	CCTCAGC	1 cut(s)	1386
BbvI	GCAGC	7 cut(s)	83, 997, 1301, 1488, 1523, 1636, 2500
BccI	CCATC	7 cut(s)	71, 98, 335, 1144, 1501, 2335, 2570
BceAI	ACGGC	4 cut(s)	142, 317, 322, 2900
BcgI	CGANNNNNNTGC	2 cut(s)	2602, 2636
BciT130I	CCWGG	5 cut(s)	257, 403, 1520, 1829, 1910
BciVI	GTATCC	2 cut(s)	1143, 1516
BclI	TGATCA	1 cut(s)	336
BcoDI	GTCTC	5 cut(s)	619, 902, 1416, 2788, 2955
BfaI	CTAG	3 cut(s)	1446, 1500, 2595
BfmI	CTRYAG	3 cut(s)	848, 2711, 2810
BfoI	RGCGCY	3 cut(s)	357, 602, 2559
BfuAI	ACCTGC	2 cut(s)	2803, 2817
BfuI	GTATCC	2 cut(s)	1143, 1516
BglII	AGATCT	1 cut(s)	2336
BisI	GCNGC	9 cut(s)	97, 602, 1011, 1290, 1477, 1512, 1650, 2514, 2999
BlsI	GCNGC	9 cut(s)	98, 603, 1012, 1291, 1478, 1513, 1651, 2515, 3000
BmcAI	AGTACT	2 cut(s)	2043, 2068
Bme1390I	CCNGG	5 cut(s)	257, 403, 1520, 1829, 1910
Bme18I	GGWCC	5 cut(s)	253, 670, 1825, 2632, 2735
BmeT110I	CYCGRG	1 cut(s)	1295
BmgT120I	GGNCC	6 cut(s)	253, 670, 1065, 1825, 2632, 2735
BmrFI	CCNGG	5 cut(s)	257, 403, 1520, 1829, 1910
BmsI	GCATC	8 cut(s)	850, 950, 969, 1106, 1402, 1717, 2173, 2572
BpiI	GAAGAC	1 cut(s)	2165
BplI	GAGNNNNNCTC	6 cut(s)	258, 290, 1027, 1059, 2123, 2155
BpmI	CTGGAG	5 cut(s)	167, 224, 972, 1613, 1850
Bpu10I	CCTNAGC	3 cut(s)	1386, 2274, 2676
BpuEI	CTTGAG	3 cut(s)	1086, 1201, 1778
BsaBI	GATNNNNATC	1 cut(s)	801
BsaHI	GRCGYC	2 cut(s)	354, 1077
BsaI	GGTCTC	2 cut(s)	619, 902
BsaJI	CCNNGG	7 cut(s)	169, 237, 1080, 2313, 2384, 2696, 2982
BsaWI	WCCGGW	6 cut(s)	127, 475, 626, 1174, 2152, 2342
BsaXI	ACNNNNNCTCC	8 cut(s)	69, 99, 770, 774, 800, 804, 2147, 2177
Bsc4I	CCNNNNNNNGG	8 cut(s)	319, 320, 1805, 2459, 2656, 2732, 2745, 2886
Bse1I	ACTGG	6 cut(s)	1275, 1484, 1558, 2754, 2862, 2904
Bse3DI	GCAATG	2 cut(s)	862, 1983
Bse8I	GATNNNNATC	1 cut(s)	801
BseAI	TCCGGA	6 cut(s)	127, 475, 626, 1174, 2152, 2342
BseBI	CCWGG	5 cut(s)	257, 403, 1520, 1829, 1910
BseDI	CCNNGG	7 cut(s)	169, 237, 1080, 2313, 2384, 2696, 2982
BseGI	GGATG	4 cut(s)	484, 1987, 2089, 2188
BseJI	GATNNNNATC	1 cut(s)	801
BseLI	CCNNNNNNNGG	8 cut(s)	319, 320, 1805, 2459, 2656, 2732, 2745, 2886
BseMI	GCAATG	2 cut(s)	862, 1983
BseMII	CTCAG	9 cut(s)	935, 1269, 1377, 1764, 2084, 2154, 2265, 2450, 2690
BseNI	ACTGG	6 cut(s)	1275, 1484, 1558, 2754, 2862, 2904
BseRI	GAGGAG	6 cut(s)	77, 124, 263, 1295, 1634, 2089
BseSI	GKGCMC	3 cut(s)	326, 568, 2946
BseXI	GCAGC	7 cut(s)	83, 997, 1301, 1488, 1523, 1636, 2500
BsgI	GTGCAG	2 cut(s)	1308, 1512
Bsh1236I	CGCG	4 cut(s)	200, 218, 265, 2301
Bsh1285I	CGRYCG	3 cut(s)	148, 379, 788
BshFI	GGCC	4 cut(s)	309, 1067, 1379, 1952
BshNI	GGYRCC	3 cut(s)	323, 353, 2527
BsiEI	CGRYCG	3 cut(s)	148, 379, 788
BsiHKAI	GWGCWC	9 cut(s)	33, 273, 568, 922, 1089, 1881, 2677, 2879, 2946
BsiHKCI	CYCGRG	1 cut(s)	1295
BsiSI	CCGG	8 cut(s)	128, 138, 427, 476, 627, 1175, 2153, 2343
BsiWI	CGTACG	2 cut(s)	72, 195
BslFI	GGGAC	7 cut(s)	386, 453, 1405, 1822, 2272, 2672, 2748
BslI	CCNNNNNNNGG	8 cut(s)	319, 320, 1805, 2459, 2656, 2732, 2745, 2886
BsmAI	GTCTC	5 cut(s)	619, 902, 1416, 2788, 2955
BsmFI	GGGAC	7 cut(s)	386, 453, 1405, 1822, 2272, 2672, 2748
BsmI	GAATGC	2 cut(s)	889, 1479
BsnI	GGCC	4 cut(s)	309, 1067, 1379, 1952
Bso31I	GGTCTC	2 cut(s)	619, 902
BsoBI	CYCGRG	1 cut(s)	1295
Bsp13I	TCCGGA	6 cut(s)	127, 475, 626, 1174, 2152, 2342
Bsp1407I	TGTACA	1 cut(s)	928
Bsp19I	CCATGG	1 cut(s)	1080
Bsp68I	TCGCGA	1 cut(s)	265
BspACI	CCGC	7 cut(s)	148, 155, 218, 379, 412, 602, 2999
BspANI	GGCC	4 cut(s)	309, 1067, 1379, 1952
BspCNI	CTCAG	9 cut(s)	934, 1270, 1378, 1765, 2083, 2153, 2266, 2449, 2689
BspEI	TCCGGA	6 cut(s)	127, 475, 626, 1174, 2152, 2342
BspFNI	CGCG	4 cut(s)	200, 218, 265, 2301
BspMAI	CTGCAG	1 cut(s)	2814
BspMI	ACCTGC	2 cut(s)	2803, 2817
BspPI	GGATC	6 cut(s)	115, 791, 1166, 1510, 1808, 3001
BspQI	GCTCTTC	1 cut(s)	2190
BspT107I	GGYRCC	3 cut(s)	323, 353, 2527
BspTNI	GGTCTC	2 cut(s)	619, 902
BsrBI	CCGCTC	1 cut(s)	150
BsrDI	GCAATG	2 cut(s)	862, 1983
BsrGI	TGTACA	1 cut(s)	928
BsrI	ACTGG	6 cut(s)	1275, 1484, 1558, 2754, 2862, 2904
BssECI	CCNNGG	7 cut(s)	169, 237, 1080, 2313, 2384, 2696, 2982
BssNI	GRCGYC	2 cut(s)	354, 1077
BssSI	CACGAG	1 cut(s)	367
BssT1I	CCWWGG	4 cut(s)	237, 1080, 2313, 2696
Bst2BI	CACGAG	1 cut(s)	367
Bst2UI	CCWGG	5 cut(s)	257, 403, 1520, 1829, 1910
Bst6I	CTCTTC	4 cut(s)	526, 905, 1392, 2190
BstACI	GRCGYC	2 cut(s)	354, 1077
BstAUI	TGTACA	1 cut(s)	928
BstC8I	GCNNGC	5 cut(s)	1950, 1974, 2202, 2238, 2280
BstDSI	CCRYGG	3 cut(s)	169, 1080, 2982
BstEII	GGTNACC	1 cut(s)	2857
BstF5I	GGATG	4 cut(s)	484, 1987, 2089, 2188
BstFNI	CGCG	4 cut(s)	200, 218, 265, 2301
BstH2I	RGCGCY	3 cut(s)	357, 602, 2559
BstHHI	GCGC	4 cut(s)	202, 356, 601, 2558
BstMAI	GTCTC	5 cut(s)	619, 902, 1416, 2788, 2955
BstMCI	CGRYCG	3 cut(s)	148, 379, 788
BstMWI	GCNNNNNNNGC	4 cut(s)	147, 353, 1568, 2883
BstNI	CCWGG	5 cut(s)	257, 403, 1520, 1829, 1910
BstNSI	RCATGY	3 cut(s)	1937, 1948, 2875
BstPI	GGTNACC	1 cut(s)	2857
BstSCI	CCNGG	5 cut(s)	255, 401, 1518, 1827, 1908
BstSFI	CTRYAG	3 cut(s)	848, 2711, 2810
BstSLI	GKGCMC	3 cut(s)	326, 568, 2946
BstUI	CGCG	4 cut(s)	200, 218, 265, 2301
BstV1I	GCAGC	7 cut(s)	83, 997, 1301, 1488, 1523, 1636, 2500
BstV2I	GAAGAC	1 cut(s)	2165
BstX2I	RGATCY	3 cut(s)	107, 796, 2336
BstYI	RGATCY	3 cut(s)	107, 796, 2336
BsuI	GTATCC	2 cut(s)	1143, 1516
BsuRI	GGCC	4 cut(s)	309, 1067, 1379, 1952
BtgI	CCRYGG	3 cut(s)	169, 1080, 2982
BtgZI	GCGATG	2 cut(s)	313, 1617
BtsCI	GGATG	4 cut(s)	484, 1987, 2089, 2188
BtsI	GCAGTG	1 cut(s)	1380
BtsIMutI	CAGTG	8 cut(s)	16, 566, 1380, 1572, 2282, 2761, 2869, 2911
BtuMI	TCGCGA	1 cut(s)	265
BveI	ACCTGC	2 cut(s)	2803, 2817
Cac8I	GCNNGC	5 cut(s)	1950, 1974, 2202, 2238, 2280
CaiI	CAGNNNCTG	2 cut(s)	42, 944
CfoI	GCGC	4 cut(s)	202, 356, 601, 2558
Cfr13I	GGNCC	6 cut(s)	253, 670, 1065, 1825, 2632, 2735
CseI	GACGC	1 cut(s)	1085
CsiI	ACCWGGT	1 cut(s)	1908
CspCI	CAANNNNNGTGG	6 cut(s)	756, 791, 1453, 1488, 2505, 2540
CviAII	CATG	9 cut(s)	498, 1081, 1819, 1934, 1945, 2231, 2267, 2620, 2872
DinI	GGCGCC	1 cut(s)	355
DraIII	CACNNNGTG	1 cut(s)	2950
DrdI	GACNNNNNNGTC	1 cut(s)	2540
DseDI	GACNNNNNNGTC	1 cut(s)	2540
Eam1104I	CTCTTC	4 cut(s)	526, 905, 1392, 2190
EarI	CTCTTC	4 cut(s)	526, 905, 1392, 2190
EciI	GGCGGA	1 cut(s)	144
Ecl136II	GAGCTC	4 cut(s)	31, 271, 1879, 2675
Eco130I	CCWWGG	4 cut(s)	237, 1080, 2313, 2696
Eco24I	GRGCYC	4 cut(s)	33, 273, 1881, 2677
Eco31I	GGTCTC	2 cut(s)	619, 902
Eco47I	GGWCC	5 cut(s)	253, 670, 1825, 2632, 2735
Eco47III	AGCGCT	1 cut(s)	2557
Eco53kI	GAGCTC	4 cut(s)	31, 271, 1879, 2675
Eco57I	CTGAAG	1 cut(s)	1548
Eco88I	CYCGRG	1 cut(s)	1295
Eco91I	GGTNACC	1 cut(s)	2857
EcoICRI	GAGCTC	4 cut(s)	31, 271, 1879, 2675
EcoO109I	RGGNCCY	4 cut(s)	253, 1065, 2632, 2735
EcoO65I	GGTNACC	1 cut(s)	2857
EcoRI	GAATTC	1 cut(s)	550
EcoRII	CCWGG	5 cut(s)	255, 401, 1518, 1827, 1908
EcoT14I	CCWWGG	4 cut(s)	237, 1080, 2313, 2696
EcoT38I	GRGCYC	4 cut(s)	33, 273, 1881, 2677
EgeI	GGCGCC	1 cut(s)	355
EheI	GGCGCC	1 cut(s)	355
ErhI	CCWWGG	4 cut(s)	237, 1080, 2313, 2696
FaeI	CATG	9 cut(s)	501, 1084, 1822, 1937, 1948, 2234, 2270, 2623, 2875
FalI	AAGNNNNNCTT	6 cut(s)	221, 253, 987, 1019, 1530, 1562
FaqI	GGGAC	7 cut(s)	386, 453, 1405, 1822, 2272, 2672, 2748
FatI	CATG	9 cut(s)	497, 1080, 1818, 1933, 1944, 2230, 2266, 2619, 2871
FauI	CCCGC	2 cut(s)	211, 405
FauNDI	CATATG	3 cut(s)	515, 748, 1032
FbaI	TGATCA	1 cut(s)	336
FblI	GTMKAC	1 cut(s)	363
Fnu4HI	GCNGC	9 cut(s)	97, 602, 1011, 1290, 1477, 1512, 1650, 2514, 2999
FokI	GGATG	4 cut(s)	491, 1994, 2096, 2195
FriOI	GRGCYC	4 cut(s)	33, 273, 1881, 2677
Fsp4HI	GCNGC	9 cut(s)	97, 602, 1011, 1290, 1477, 1512, 1650, 2514, 2999
FspBI	CTAG	3 cut(s)	1446, 1500, 2595
GlaI	GCGC	4 cut(s)	201, 355, 600, 2557
GluI	GCNGC	9 cut(s)	97, 602, 1011, 1290, 1477, 1512, 1650, 2514, 2999
GsuI	CTGGAG	5 cut(s)	167, 224, 972, 1613, 1850
HaeII	RGCGCY	3 cut(s)	357, 602, 2559
HaeIII	GGCC	4 cut(s)	309, 1067, 1379, 1952
HapII	CCGG	8 cut(s)	128, 138, 427, 476, 627, 1175, 2153, 2343
HgaI	GACGC	1 cut(s)	1085
HhaI	GCGC	4 cut(s)	202, 356, 601, 2558
Hin1I	GRCGYC	2 cut(s)	354, 1077
Hin1II	CATG	9 cut(s)	501, 1084, 1822, 1937, 1948, 2234, 2270, 2623, 2875
Hin6I	GCGC	4 cut(s)	200, 354, 599, 2556
HinP1I	GCGC	4 cut(s)	200, 354, 599, 2556
HincII	GTYRAC	1 cut(s)	1915
HindII	GTYRAC	1 cut(s)	1915
HindIII	AAGCTT	2 cut(s)	683, 2261
HpaII	CCGG	8 cut(s)	128, 138, 427, 476, 627, 1175, 2153, 2343
HphI	GGTGA	4 cut(s)	496, 2424, 2851, 2863
Hpy166II	GTNNAC	7 cut(s)	7, 364, 566, 670, 1915, 2450, 2944
Hpy8I	GTNNAC	7 cut(s)	7, 364, 566, 670, 1915, 2450, 2944
Hpy99I	CGWCG	4 cut(s)	52, 218, 437, 843
HpyCH4IV	ACGT	2 cut(s)	213, 2170
HpyF10VI	GCNNNNNNNGC	4 cut(s)	147, 353, 1568, 2883
HpySE526I	ACGT	2 cut(s)	213, 2170
Hsp92I	GRCGYC	2 cut(s)	354, 1077
Hsp92II	CATG	9 cut(s)	501, 1084, 1822, 1937, 1948, 2234, 2270, 2623, 2875
HspAI	GCGC	4 cut(s)	200, 354, 599, 2556
KasI	GGCGCC	1 cut(s)	353
KflI	GGGWCCC	1 cut(s)	2735
Kpn2I	TCCGGA	6 cut(s)	127, 475, 626, 1174, 2152, 2342
Ksp22I	TGATCA	1 cut(s)	336
LguI	GCTCTTC	1 cut(s)	2190
Lsp1109I	GCAGC	7 cut(s)	83, 997, 1301, 1488, 1523, 1636, 2500
LweI	GCATC	8 cut(s)	850, 950, 969, 1106, 1402, 1717, 2173, 2572
MabI	ACCWGGT	1 cut(s)	1908
MaeI	CTAG	3 cut(s)	1446, 1500, 2595
MaeII	ACGT	2 cut(s)	213, 2170
MaeIII	GTNAC	6 cut(s)	173, 762, 865, 1486, 2857, 2864
MbiI	CCGCTC	1 cut(s)	150
MfeI	CAATTG	3 cut(s)	753, 1779, 1872
MflI	RGATCY	3 cut(s)	107, 796, 2336
Mly113I	GGCGCC	1 cut(s)	354
MlyI	GAGTC	6 cut(s)	125, 299, 1307, 1784, 2550, 2957
MmeI	TCCRAC	4 cut(s)	168, 1399, 1886, 2201
MroI	TCCGGA	6 cut(s)	127, 475, 626, 1174, 2152, 2342
MseI	TTAA	8 cut(s)	935, 1452, 1593, 1766, 2063, 2379, 2478, 2817
MslI	CAYNNNNRTG	2 cut(s)	332, 1325
MspI	CCGG	8 cut(s)	128, 138, 427, 476, 627, 1175, 2153, 2343
MspR9I	CCNGG	5 cut(s)	257, 403, 1520, 1829, 1910
MunI	CAATTG	3 cut(s)	753, 1779, 1872
Mva1269I	GAATGC	2 cut(s)	889, 1479
MvaI	CCWGG	5 cut(s)	257, 403, 1520, 1829, 1910
MvnI	CGCG	4 cut(s)	200, 218, 265, 2301
MwoI	GCNNNNNNNGC	4 cut(s)	147, 353, 1568, 2883
NarI	GGCGCC	1 cut(s)	354
NcoI	CCATGG	1 cut(s)	1080
NdeI	CATATG	3 cut(s)	515, 748, 1032
NlaIII	CATG	9 cut(s)	501, 1084, 1822, 1937, 1948, 2234, 2270, 2623, 2875
NmuCI	GTSAC	3 cut(s)	173, 2857, 2864
NruI	TCGCGA	1 cut(s)	265
NspI	RCATGY	3 cut(s)	1937, 1948, 2875
PaeR7I	CTCGAG	1 cut(s)	1295
PciSI	GCTCTTC	1 cut(s)	2190
PcsI	WCGNNNNNNNCGW	3 cut(s)	63, 99, 790
PctI	GAATGC	2 cut(s)	889, 1479
Pfl23II	CGTACG	2 cut(s)	72, 195
PflFI	GACNNNGTC	1 cut(s)	1910
PflMI	CCANNNNNTGG	1 cut(s)	2459
PfoI	TCCNGGA	3 cut(s)	255, 1518, 1827
PkrI	GCNGC	9 cut(s)	98, 603, 1012, 1291, 1478, 1513, 1651, 2515, 3000
PleI	GAGTC	6 cut(s)	125, 298, 1306, 1783, 2549, 2957
PluTI	GGCGCC	1 cut(s)	357
PpsI	GAGTC	6 cut(s)	125, 298, 1306, 1783, 2549, 2957
PpuMI	RGGWCCY	3 cut(s)	253, 2632, 2735
Psp124BI	GAGCTC	4 cut(s)	33, 273, 1881, 2677
Psp5II	RGGWCCY	3 cut(s)	253, 2632, 2735
Psp6I	CCWGG	5 cut(s)	255, 401, 1518, 1827, 1908
PspEI	GGTNACC	1 cut(s)	2857
PspGI	CCWGG	5 cut(s)	255, 401, 1518, 1827, 1908
PspLI	CGTACG	2 cut(s)	72, 195
PspPI	GGNCC	6 cut(s)	253, 670, 1065, 1825, 2632, 2735
PspPPI	RGGWCCY	3 cut(s)	253, 2632, 2735
PspXI	VCTCGAGB	1 cut(s)	1295
PstI	CTGCAG	1 cut(s)	2814
PstNI	CAGNNNCTG	2 cut(s)	42, 944
PsuI	RGATCY	3 cut(s)	107, 796, 2336
PsyI	GACNNNGTC	1 cut(s)	1910
RruI	TCGCGA	1 cut(s)	265
RseI	CAYNNNNRTG	2 cut(s)	332, 1325
SacI	GAGCTC	4 cut(s)	33, 273, 1881, 2677
SapI	GCTCTTC	1 cut(s)	2190
SaqAI	TTAA	8 cut(s)	935, 1452, 1593, 1766, 2063, 2379, 2478, 2817
SatI	GCNGC	9 cut(s)	97, 602, 1011, 1290, 1477, 1512, 1650, 2514, 2999
Sau96I	GGNCC	6 cut(s)	253, 670, 1065, 1825, 2632, 2735
SbfI	CCTGCAGG	1 cut(s)	2814
ScaI	AGTACT	2 cut(s)	2043, 2068
SchI	GAGTC	6 cut(s)	125, 299, 1307, 1784, 2550, 2957
ScrFI	CCNGG	5 cut(s)	257, 403, 1520, 1829, 1910
SdaI	CCTGCAGG	1 cut(s)	2814
SexAI	ACCWGGT	1 cut(s)	1908
SfaNI	GCATC	8 cut(s)	850, 950, 969, 1106, 1402, 1717, 2173, 2572
SfcI	CTRYAG	3 cut(s)	848, 2711, 2810
SfoI	GGCGCC	1 cut(s)	355
Sfr274I	CTCGAG	1 cut(s)	1295
SinI	GGWCC	5 cut(s)	253, 670, 1825, 2632, 2735
SlaI	CTCGAG	1 cut(s)	1295
SmiMI	CAYNNNNRTG	2 cut(s)	332, 1325
SmlI	CTYRAG	4 cut(s)	1101, 1216, 1295, 1793
SmoI	CTYRAG	4 cut(s)	1101, 1216, 1295, 1793
Sse8387I	CCTGCAGG	1 cut(s)	2814
SsiI	CCGC	7 cut(s)	148, 155, 218, 379, 412, 602, 2999
SspDI	GGCGCC	1 cut(s)	353
SspI	AATATT	2 cut(s)	655, 1897
SspMI	CTAG	3 cut(s)	1446, 1500, 2595
SstI	GAGCTC	4 cut(s)	33, 273, 1881, 2677
StyD4I	CCNGG	5 cut(s)	255, 401, 1518, 1827, 1908
StyI	CCWWGG	4 cut(s)	237, 1080, 2313, 2696
TaiI	ACGT	2 cut(s)	216, 2173
TaqI	TCGA	7 cut(s)	27, 57, 66, 293, 491, 1296, 2612
TatI	WGTACW	3 cut(s)	928, 2041, 2066
TauI	GCSGC	2 cut(s)	604, 3001
Tru1I	TTAA	8 cut(s)	935, 1452, 1593, 1766, 2063, 2379, 2478, 2817
Tru9I	TTAA	8 cut(s)	935, 1452, 1593, 1766, 2063, 2379, 2478, 2817
TscAI	CASTG	8 cut(s)	16, 573, 1387, 1572, 2289, 2761, 2869, 2911
TseFI	GTSAC	3 cut(s)	173, 2857, 2864
TseI	GCWGC	7 cut(s)	96, 1010, 1289, 1476, 1511, 1649, 2513
Tsp45I	GTSAC	3 cut(s)	173, 2857, 2864
TspGWI	ACGGA	2 cut(s)	90, 2195
TspRI	CASTG	8 cut(s)	16, 573, 1387, 1572, 2289, 2761, 2869, 2911
Tth111I	GACNNNGTC	1 cut(s)	1910
Van91I	CCANNNNNTGG	1 cut(s)	2459
VneI	GTGCAC	2 cut(s)	564, 2942
VpaK11BI	GGWCC	5 cut(s)	253, 670, 1825, 2632, 2735
XapI	RAATTY	4 cut(s)	464, 550, 2370, 2422
XceI	RCATGY	3 cut(s)	1937, 1948, 2875
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1825
XhoI	CTCGAG	1 cut(s)	1295
XmiI	GTMKAC	1 cut(s)	363
XspI	CTAG	3 cut(s)	1446, 1500, 2595
ZrmI	AGTACT	2 cut(s)	2043, 2068

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.