Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0009569

Physical Location & Seq

Genomic Coordinates Reverse (-)

13151114 .. 13156488

Gene Structure 5375 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ47618

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3036 bp

ATGGTTCACAGTGCCTATGATTCTGTCGAGCTCATCTCCGACTGCCCCACCAAGATCGAAGCCATCGAATCCTACGGCCCTAAGCTCCTCCTCGGCTGCTCCGATGGATCCCTCCGAATCTACGCTCCAGATTCCTCCGGCTCCCGCTCCCCACCCTCCGACTATCACACTCAATCCCTCCAAAAGGAACCCTACTCCCTCGAGAGAAACATCGCCGGGTTCTCTAAGAAGCCGCTGCTCTCTATGGAGGTCTTGGAATCCAGAGAGCTCCTTTTGTCCCTCTCCGAAACGATATCGTTTCACGGCCTCCCGAATTTGGGCACCATCGCCGTGATCACCAAGGCCAAGGGCGCCAATGTGTACTCCTGGGACGACCGCCGCGGATTCCTGTGCTTCGCCAGGCAGAAGAAAGTCTGTATTTTCCGACACGACGGTGGAAGAGGTTTCGTGGAGGTGAAGGAATTCGGAGTTCCGGATGTGGTGAAGTCTATGTCATGGTGTGGAGACAATATATGTATAGGGATTAGGAGAGATTATATGATACTCAATTCTACAACTGGTGCATTGACTGATGTGTTTCCTTCGGGGAGGCTGGCTCCGCCGCTGGTGGTTCCTCTTCCTTCCGGGGAGCTTCTTCTCGGCAAGGATAACATTGGGGTTTTTGTGGACCAAAATGGGAAACTTGCTCATGAAGGCAGGGTTTGTTGGTCAGAGGCGCCTACGGTTGTTGTTATTCAGAAGGCCTACGGAATAGCTCTGTTATCGAGATATGTTGAGGTTCGGTCCCTACGAGCGCCATATCCATTGATACAAACGGTTGTTCTTCGAAATGCTCGTCGTCTTCTCCAAAGCAACAACGCTGCTATTGTTGCATTAGACTATGCTGTTTATGGTCTCTTCCCTGTCCCTCTTGGTGCCCAGATTGTACAATTAACAGCATCTGGCGAGTTTGAGGAAGCTTTGTCATTATGTAAGCTGCTTCCGCCTGAAGAAGCAAGCCTTAGAGCTGCAAAGGAGGCTTCAATCCATATAAGATGTGCTCACCATCGTTTTGAAACTGGGGACTATGAGGATGCCATGGAGCATTTCGTGGCATCTCAAGTAGATATAACCTATGTACTTTCTATGTATCCTTCTATTGTTCTTCCTAAGACAACCATGATATCCGATCCAGACAAACTGATGGATATATCTGGGGACTCATCATATCTCTCAAGAGGTTCATCTGGTATGTCAGATGATATGGAACCCTCTGCACTGTCACATGTGCTGGAATCAGAGGAGAGTGCAGCACTTGAGTCCAAAAAAATGAGCCATAACACTCTCATGGCTCTCATTAAGTTCTTGCAGAAAAAGAGATTCAGCATAATTGAAAAAGCTACTGCTGAGGGGACAGAAGAGGTTGTTTTGGATGCTGTTGGAGACAAATTCGCATCCCATGAGTCTAATAGGTATAAGAAGACAAACAAGGGGCGTGGCAGCATTCCAGTTACCTCCCGTGCTAGGGAGATGGCAGCTATACTGGATACAGCCCTACTTCAAGCTCTACTTCTTACTGGACAGTCTTCAGCGGCCGTGGAATTACTGAAAGGTCTTAATTACTGTGATGTAAAAATATGCGAGGAGATTCTCCTAAAAAGTAATCATCATGCTGCTTTGTTAGAACTTTACAAGTGCAATTCAATGCACCATGAAGCTCTAAAACTTTTGCATCAGTTAGTAGAAGAGTCCAAATCTAATCAAGTGCAACCTGAGGTGATCCAAAAGATAAAGCCGGAGTCAATTGTTGAATATCTCAAGCCTCTTTGTGGCACTGATCCCATGCTCGTCTTGGAGTATTCAATGCTTGTCCTTGAAAGTTGCCCAACACAGACAATTGAGCTCTTTTTGAATGGAAATATTCCAGCTGATTTGGTAAACTCTTATTTGAAACAGCATGCTCCAAACATGCAGGCCAGATACTTGGAACTTATGCTTGCTATGGATGAAAATGGGATATCCGGGAATCTGCAAAATGAAATGGTAAATATCTATCTATCAGAAGTCCTTGACTGGTATGCAGATTTAAGTGCTCAGCAGAAATGGGAGGAGCAAACTTACTCCACGACGAGAAAGAAATTATTGTCTGCTTTAGAGAGTATATCAGGTTATAGTCCAGAGGCTTTGCTGAAACGTCTTCCGTCTGATGCTTTATATGAAGAGCATGCAATTTTGCTGGGCAAAATGAACCAACATGAGCTTGCCTTATCCCTATATGTTCACAAGCTTCATCTTCCTGAGATGGCACTGTCCTATTGTGATCGGGTGTATGATTCGTTGGCGCATCAACCGTCTTCAAGATCTTCTGGCAATATATACCTTACTCTATTGCAAATTTATCTTAATCCGAAGAGAACAACCAAGAACTTTGAGAAGCGGATTACGAATTTGGTATCACCGCAGAACATAGGAACACCAAAGGCTGGTTCTGCAAATACAGTTAAAAGTAAAGGAGGTCGTGGAGCTAAGAAAATTGCTGCAATAGAGGTGGCAGATGACATAAGAGTCGGTCAAAGTGGCACTGACAGTAGCAGGAGTGATGGAGATGCAGATGCAGATGCAGATGCTGACGAGTCTGGTGAGGAAGGAGGGTCAGCAATCATGCTTGATGAGGTTCTTGATTTGTTGAGCAGAAGGTGGGACAGAATAAATGGAGCTCAAGCTCTCAAACTTTTGCCAAGGGAGACTAAACTACAGAACATGCTTCCGTTTATGGGACCTCTTTTGAGAAAATCCAGTGAAGCTTACAGGAACCTATCAGTGATAAAAAGTCTAAGACAAAGCGATAACCTGCAGGTTAAAGAAGAACTCTACGAAAAAAGGAAAGGGGTGGTAAAGATCACGAGTGACAGCATGTGCTCCCTCTGCAGAAAGAAAATAGGGACAAGTGTTTTTGCTGTGTATCCGAATGGGAGTACAATAGTGCATTTTGTTTGCTTCAGAGACTCCCAAAGTATGAAAGCAGTGGGCAGAGGATCGCCCTTAAGGAAGCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0000325 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005770 GO:0005773 GO:0005774 GO:0005911 GO:0006810 GO:0006906 GO:0006914 GO:0006996 GO:0007033 GO:0007034 GO:0007041 GO:0008150 GO:0008152 GO:0008333 GO:0009056 GO:0009506 GO:0009705 GO:0009987 GO:0010008 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016197 GO:0030054 GO:0030897 GO:0031090 GO:0031410 GO:0031902 GO:0031982 GO:0032991 GO:0034058 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044248 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048284 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055044 GO:0061024 GO:0061025 GO:0061919 GO:0071840 GO:0090174 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:0098876 GO:0099023 GO:1902774 GO:1990126

KEGG Pathways

Metabolic & Signaling

ko04138 map04138

Pfam Domains

Protein Families

CNH Clathrin Vps39_1 Vps39_2

Protein Analysis

1011

Amino Acids

111.76

Weight (kDa)

6.43

Isoelectric Point (pI)

47.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CNH	PF00780	28 - 281	3.4e-20	CNH domain
Clathrin	PF00637	505 - 599	2.3e-06	Region in Clathrin and VPS
Vps39_1	PF10366	508 - 618	2.7e-25	Vacuolar sorting protein 39 domain 1
zf-Vps39_C	PF10367	957 - 995	1.3e-17	Vacuolar sorting protein 39 C-terminal zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009569)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36630 AT4G36630
fragaria_vesca	FvH4_1g13311
malus_domestica	MD02G1147600.v1.1 MD15G1261900.v1.1
prunus_persica	Prupe.7G154500_v2.0.a1
pyrus_communis	pycom02g11660 pycom15g22910
rosa_chinensis	RchiOBHm_Chr2g0101111 RchiOBHm_Chr2g0101581
rosa_laevigata	RLG00000017092
rosa_multiflora	Rmu_sc0006153.1_g000006 Rmu_sc0019613.1_g000003
rosa_roxburghii	Rroxscaffold_2G00141170
rosa_rugosa	Rorug02G0097300 Rorug02G0100900
rosa_samantha	Rh2AG148200 Rh2BG153600 Rh2CG149800 Rh2CG153900 Rh2DG153900
rosa_wichuraiana	Rw2G011560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2543
Acc36I	ACCTGC	2 cut(s)	2824, 2838
AccB1I	GGYRCC	4 cut(s)	320, 350, 715, 914
AccB7I	CCANNNNNTGG	1 cut(s)	2462
AccBSI	CCGCTC	1 cut(s)	147
AccII	CGCG	1 cut(s)	381
AccIII	TCCGGA	1 cut(s)	472
AclWI	GGATC	6 cut(s)	102, 115, 1163, 1753, 1811, 3022
AcoI	YGGCCR	1 cut(s)	1572
AcsI	RAATTY	5 cut(s)	313, 461, 1427, 2373, 2425
AcuI	CTGAAG	3 cut(s)	1008, 1551, 2962
AcyI	GRCGYC	2 cut(s)	351, 716
AfaI	GTAC	4 cut(s)	362, 927, 1119, 2956
AfiI	CCNNNNNNNGG	8 cut(s)	184, 316, 317, 1503, 1504, 1808, 2462, 2753
AflII	CTTAAG	1 cut(s)	3022
AflIII	ACRYGT	1 cut(s)	1264
AjnI	CCWGG	2 cut(s)	365, 398
AjuI	GAANNNNNNNTTGG	2 cut(s)	2449, 2481
AleI	CACNNNNGTG	1 cut(s)	432
Alw21I	GWGCWC	7 cut(s)	33, 270, 1042, 1884, 2074, 2698, 2900
Alw26I	GTCTC	5 cut(s)	498, 899, 1416, 2717, 2976
AlwI	GGATC	6 cut(s)	102, 115, 1163, 1753, 1811, 3022
AlwNI	CAGNNNCTG	2 cut(s)	941, 2606
Ama87I	CYCGRG	1 cut(s)	200
Aor13HI	TCCGGA	1 cut(s)	472
AoxI	GGCC	6 cut(s)	76, 304, 342, 741, 1572, 1953
ApoI	RAATTY	5 cut(s)	313, 461, 1427, 2373, 2425
ArsI	GACNNNNNNTTYG	2 cut(s)	820, 852
Asp700I	GAANNNNTTC	1 cut(s)	461
AspLEI	GCGC	4 cut(s)	353, 718, 796, 2323
AspS9I	GGNCC	4 cut(s)	77, 667, 783, 2756
AsuC2I	CCSGG	3 cut(s)	217, 625, 2002
AsuHPI	GGTGA	7 cut(s)	328, 466, 493, 1034, 1768, 2427, 2630
AsuII	TTCGAA	1 cut(s)	826
AvaI	CYCGRG	1 cut(s)	200
AvaII	GGWCC	3 cut(s)	667, 783, 2756
AxyI	CCTNAGG	1 cut(s)	1752
BaeGI	GKGCMC	2 cut(s)	323, 919
BamHI	GGATCC	1 cut(s)	107
BanI	GGYRCC	4 cut(s)	320, 350, 715, 914
BanII	GRGCYC	4 cut(s)	33, 270, 1884, 2698
BauI	CACGAG	1 cut(s)	2881
BbsI	GAAGAC	5 cut(s)	833, 1466, 1557, 2168, 2325
Bbv12I	GWGCWC	7 cut(s)	33, 270, 1042, 1884, 2074, 2698, 2900
BbvCI	CCTCAGC	1 cut(s)	1386
BccI	CCATC	8 cut(s)	71, 98, 332, 1053, 1177, 1504, 2275, 2573
BceAI	ACGGC	4 cut(s)	91, 314, 319, 1559
BciT130I	CCWGG	2 cut(s)	367, 400
BciVI	GTATCC	3 cut(s)	1140, 1519, 2952
BclI	TGATCA	1 cut(s)	333
BcnI	CCSGG	3 cut(s)	217, 625, 2002
BcoDI	GTCTC	5 cut(s)	498, 899, 1416, 2717, 2976
BfaI	CTAG	1 cut(s)	1503
BfmI	CTRYAG	3 cut(s)	2732, 2831, 2905
BfoI	RGCGCY	3 cut(s)	354, 719, 797
BfrI	CTTAAG	1 cut(s)	3022
BfuAI	ACCTGC	2 cut(s)	2824, 2838
BfuI	GTATCC	3 cut(s)	1140, 1519, 2952
BglII	AGATCT	1 cut(s)	2339
BlpI	GCTNAGC	1 cut(s)	2073
Bme1390I	CCNGG	5 cut(s)	217, 367, 400, 625, 2002
Bme18I	GGWCC	3 cut(s)	667, 783, 2756
BmeT110I	CYCGRG	1 cut(s)	200
BmgT120I	GGNCC	4 cut(s)	77, 667, 783, 2756
BmrFI	CCNGG	5 cut(s)	217, 367, 400, 625, 2002
BmrI	ACTGGG	1 cut(s)	1068
BmuI	ACTGGG	1 cut(s)	1068
BpiI	GAAGAC	5 cut(s)	833, 1466, 1557, 2168, 2325
BplI	GAGNNNNNCTC	4 cut(s)	20, 52, 580, 612
BpmI	CTGGAG	1 cut(s)	111
Bpu10I	CCTNAGC	2 cut(s)	81, 1386
Bpu1102I	GCTNAGC	1 cut(s)	2073
Bpu14I	TTCGAA	1 cut(s)	826
BpuEI	CTTGAG	5 cut(s)	1083, 1198, 1316, 1781, 2682
BpuMI	CCSGG	3 cut(s)	217, 625, 2002
BsaHI	GRCGYC	2 cut(s)	351, 716
BsaI	GGTCTC	1 cut(s)	899
BsaJI	CCNNGG	9 cut(s)	91, 339, 345, 366, 379, 624, 1077, 1575, 2717
BsaWI	WCCGGW	1 cut(s)	472
Bsc4I	CCNNNNNNNGG	8 cut(s)	184, 316, 317, 1503, 1504, 1808, 2462, 2753
Bse1I	ACTGG	7 cut(s)	562, 1063, 1487, 1527, 1561, 2057, 2775
Bse21I	CCTNAGG	1 cut(s)	1752
BseAI	TCCGGA	1 cut(s)	472
BseBI	CCWGG	2 cut(s)	367, 400
BseDI	CCNNGG	9 cut(s)	91, 339, 345, 366, 379, 624, 1077, 1575, 2717
BseGI	GGATG	5 cut(s)	481, 1078, 1417, 1433, 1990
BseLI	CCNNNNNNNGG	8 cut(s)	184, 316, 317, 1503, 1504, 1808, 2462, 2753
BseMII	CTCAG	4 cut(s)	1377, 1743, 2087, 2268
BseNI	ACTGG	7 cut(s)	562, 1063, 1487, 1527, 1561, 2057, 2775
BseRI	GAGGAG	5 cut(s)	77, 80, 1295, 1637, 2102
BseSI	GKGCMC	2 cut(s)	323, 919
BseX3I	CGGCCG	1 cut(s)	1572
BseYI	CCCAGC	1 cut(s)	2215
BsgI	GTGCAG	2 cut(s)	1239, 1308
Bsh1236I	CGCG	1 cut(s)	381
Bsh1285I	CGRYCG	2 cut(s)	376, 1575
BshFI	GGCC	6 cut(s)	78, 306, 344, 743, 1574, 1955
BshNI	GGYRCC	4 cut(s)	320, 350, 715, 914
BsiEI	CGRYCG	2 cut(s)	376, 1575
BsiHKAI	GWGCWC	7 cut(s)	33, 270, 1042, 1884, 2074, 2698, 2900
BsiHKCI	CYCGRG	1 cut(s)	200
BsiSI	CCGG	6 cut(s)	138, 216, 473, 624, 1775, 2001
BslI	CCNNNNNNNGG	8 cut(s)	184, 316, 317, 1503, 1504, 1808, 2462, 2753
BsmAI	GTCTC	5 cut(s)	498, 899, 1416, 2717, 2976
BsmI	GAATGC	1 cut(s)	1482
BsnI	GGCC	6 cut(s)	78, 306, 344, 743, 1574, 1955
Bso31I	GGTCTC	1 cut(s)	899
BsoBI	CYCGRG	1 cut(s)	200
Bsp119I	TTCGAA	1 cut(s)	826
Bsp1286I	GDGCHC	9 cut(s)	33, 270, 323, 919, 1042, 1884, 2074, 2698, 2900
Bsp13I	TCCGGA	1 cut(s)	472
Bsp1407I	TGTACA	1 cut(s)	925
Bsp1720I	GCTNAGC	1 cut(s)	2073
Bsp19I	CCATGG	1 cut(s)	1077
BspANI	GGCC	6 cut(s)	78, 306, 344, 743, 1574, 1955
BspCNI	CTCAG	4 cut(s)	1378, 1744, 2086, 2269
BspEI	TCCGGA	1 cut(s)	472
BspFNI	CGCG	1 cut(s)	381
BspHI	TCATGA	1 cut(s)	688
BspMAI	CTGCAG	2 cut(s)	2835, 2909
BspMI	ACCTGC	2 cut(s)	2824, 2838
BspPI	GGATC	6 cut(s)	102, 115, 1163, 1753, 1811, 3022
BspQI	GCTCTTC	1 cut(s)	2193
BspT104I	TTCGAA	1 cut(s)	826
BspT107I	GGYRCC	4 cut(s)	320, 350, 715, 914
BspTI	CTTAAG	1 cut(s)	3022
BspTNI	GGTCTC	1 cut(s)	899
BsrBI	CCGCTC	1 cut(s)	147
BsrGI	TGTACA	1 cut(s)	925
BsrI	ACTGG	7 cut(s)	562, 1063, 1487, 1527, 1561, 2057, 2775
BssECI	CCNNGG	9 cut(s)	91, 339, 345, 366, 379, 624, 1077, 1575, 2717
BssNI	GRCGYC	2 cut(s)	351, 716
BssSI	CACGAG	1 cut(s)	2881
BssT1I	CCWWGG	4 cut(s)	339, 345, 1077, 2717
Bst2BI	CACGAG	1 cut(s)	2881
Bst2UI	CCWGG	2 cut(s)	367, 400
Bst6I	CTCTTC	7 cut(s)	433, 621, 902, 1392, 1719, 2193, 2384
BstACI	GRCGYC	2 cut(s)	351, 716
BstAFI	CTTAAG	1 cut(s)	3022
BstAUI	TGTACA	1 cut(s)	925
BstBI	TTCGAA	1 cut(s)	826
BstC8I	GCNNGC	7 cut(s)	594, 997, 1938, 1953, 1977, 2205, 2241
BstDSI	CCRYGG	3 cut(s)	379, 1077, 1575
BstENI	CCTNNNNNAGG	1 cut(s)	182
BstF5I	GGATG	5 cut(s)	481, 1078, 1417, 1433, 1990
BstFNI	CGCG	1 cut(s)	381
BstH2I	RGCGCY	3 cut(s)	354, 719, 797
BstHHI	GCGC	4 cut(s)	353, 718, 796, 2323
BstMAI	GTCTC	5 cut(s)	498, 899, 1416, 2717, 2976
BstMCI	CGRYCG	2 cut(s)	376, 1575
BstMWI	GCNNNNNNNGC	7 cut(s)	350, 598, 869, 982, 1016, 2468, 2904
BstNI	CCWGG	2 cut(s)	367, 400
BstNSI	RCATGY	6 cut(s)	1268, 1940, 1951, 2207, 2743, 2896
BstSCI	CCNGG	5 cut(s)	215, 365, 398, 623, 2000
BstSFI	CTRYAG	3 cut(s)	2732, 2831, 2905
BstSLI	GKGCMC	2 cut(s)	323, 919
BstUI	CGCG	1 cut(s)	381
BstV2I	GAAGAC	5 cut(s)	833, 1466, 1557, 2168, 2325
BstX2I	RGATCY	2 cut(s)	107, 2339
BstXI	CCANNNNNNTGG	1 cut(s)	1963
BstYI	RGATCY	2 cut(s)	107, 2339
BstZI	CGGCCG	1 cut(s)	1572
Bsu36I	CCTNAGG	1 cut(s)	1752
BsuI	GTATCC	3 cut(s)	1140, 1519, 2952
BsuRI	GGCC	6 cut(s)	78, 306, 344, 743, 1574, 1955
BtgI	CCRYGG	3 cut(s)	379, 1077, 1575
BtgZI	GCGATG	2 cut(s)	196, 310
BtsCI	GGATG	5 cut(s)	481, 1078, 1417, 1433, 1990
BtsI	GCAGTG	1 cut(s)	3009
BtsIMutI	CAGTG	8 cut(s)	16, 1256, 1812, 2285, 2559, 2782, 2805, 3009
BveI	ACCTGC	2 cut(s)	2824, 2838
Cac8I	GCNNGC	7 cut(s)	594, 997, 1938, 1953, 1977, 2205, 2241
CaiI	CAGNNNCTG	2 cut(s)	941, 2606
CciI	TCATGA	1 cut(s)	688
CfoI	GCGC	4 cut(s)	353, 718, 796, 2323
Cfr13I	GGNCC	4 cut(s)	77, 667, 783, 2756
Cfr42I	CCGCGG	1 cut(s)	382
Csp6I	GTAC	4 cut(s)	361, 926, 1118, 2955
CspCI	CAANNNNNGTGG	4 cut(s)	1456, 1491, 2508, 2543
CviQI	GTAC	4 cut(s)	361, 926, 1118, 2955
DinI	GGCGCC	2 cut(s)	352, 717
DrdI	GACNNNNNNGTC	1 cut(s)	2543
DseDI	GACNNNNNNGTC	1 cut(s)	2543
EaeI	YGGCCR	1 cut(s)	1572
EagI	CGGCCG	1 cut(s)	1572
Eam1104I	CTCTTC	7 cut(s)	433, 621, 902, 1392, 1719, 2193, 2384
EarI	CTCTTC	7 cut(s)	433, 621, 902, 1392, 1719, 2193, 2384
EciI	GGCGGA	2 cut(s)	588, 972
Ecl136II	GAGCTC	4 cut(s)	31, 268, 1882, 2696
EclXI	CGGCCG	1 cut(s)	1572
Eco130I	CCWWGG	4 cut(s)	339, 345, 1077, 2717
Eco147I	AGGCCT	1 cut(s)	743
Eco24I	GRGCYC	4 cut(s)	33, 270, 1884, 2698
Eco31I	GGTCTC	1 cut(s)	899
Eco32I	GATATC	3 cut(s)	294, 1164, 1998
Eco47I	GGWCC	3 cut(s)	667, 783, 2756
Eco52I	CGGCCG	1 cut(s)	1572
Eco53kI	GAGCTC	4 cut(s)	31, 268, 1882, 2696
Eco57I	CTGAAG	3 cut(s)	1008, 1551, 2962
Eco81I	CCTNAGG	1 cut(s)	1752
Eco88I	CYCGRG	1 cut(s)	200
EcoICRI	GAGCTC	4 cut(s)	31, 268, 1882, 2696
EcoNI	CCTNNNNNAGG	1 cut(s)	182
EcoO109I	RGGNCCY	1 cut(s)	2756
EcoRI	GAATTC	1 cut(s)	461
EcoRII	CCWGG	2 cut(s)	365, 398
EcoRV	GATATC	3 cut(s)	294, 1164, 1998
EcoT14I	CCWWGG	4 cut(s)	339, 345, 1077, 2717
EcoT38I	GRGCYC	4 cut(s)	33, 270, 1884, 2698
EgeI	GGCGCC	2 cut(s)	352, 717
EheI	GGCGCC	2 cut(s)	352, 717
ErhI	CCWWGG	4 cut(s)	339, 345, 1077, 2717
FalI	AAGNNNNNCTT	4 cut(s)	984, 1016, 1533, 1565
FauI	CCCGC	1 cut(s)	152
FbaI	TGATCA	1 cut(s)	333
FokI	GGATG	5 cut(s)	488, 1085, 1420, 1424, 1997
FriOI	GRGCYC	4 cut(s)	33, 270, 1884, 2698
FspBI	CTAG	1 cut(s)	1503
GlaI	GCGC	4 cut(s)	352, 717, 795, 2322
GsaI	CCCAGC	1 cut(s)	2219
GsuI	CTGGAG	1 cut(s)	111
HaeII	RGCGCY	3 cut(s)	354, 719, 797
HaeIII	GGCC	6 cut(s)	78, 306, 344, 743, 1574, 1955
HapII	CCGG	6 cut(s)	138, 216, 473, 624, 1775, 2001
HhaI	GCGC	4 cut(s)	353, 718, 796, 2323
Hin1I	GRCGYC	2 cut(s)	351, 716
Hin6I	GCGC	4 cut(s)	351, 716, 794, 2321
HinP1I	GCGC	4 cut(s)	351, 716, 794, 2321
HindIII	AAGCTT	3 cut(s)	957, 2264, 2781
HpaII	CCGG	6 cut(s)	138, 216, 473, 624, 1775, 2001
HphI	GGTGA	7 cut(s)	328, 466, 493, 1034, 1768, 2427, 2630
Hpy166II	GTNNAC	5 cut(s)	7, 361, 667, 1918, 2260
Hpy8I	GTNNAC	5 cut(s)	7, 361, 667, 1918, 2260
Hpy99I	CGWCG	3 cut(s)	434, 840, 2110
HpyAV	CCTTC	8 cut(s)	451, 591, 630, 686, 733, 1143, 2618, 2667
HpyCH4IV	ACGT	1 cut(s)	2173
HpyF10VI	GCNNNNNNNGC	7 cut(s)	350, 598, 869, 982, 1016, 2468, 2904
HpySE526I	ACGT	1 cut(s)	2173
Hsp92I	GRCGYC	2 cut(s)	351, 716
HspAI	GCGC	4 cut(s)	351, 716, 794, 2321
KasI	GGCGCC	2 cut(s)	350, 715
Kpn2I	TCCGGA	1 cut(s)	472
Ksp22I	TGATCA	1 cut(s)	333
KspI	CCGCGG	1 cut(s)	382
LguI	GCTCTTC	1 cut(s)	2193
MaeI	CTAG	1 cut(s)	1503
MaeII	ACGT	1 cut(s)	2173
MaeIII	GTNAC	3 cut(s)	1260, 1489, 2885
MbiI	CCGCTC	1 cut(s)	147
MfeI	CAATTG	2 cut(s)	1782, 1875
MflI	RGATCY	2 cut(s)	107, 2339
MhlI	GDGCHC	9 cut(s)	33, 270, 323, 919, 1042, 1884, 2074, 2698, 2900
Mly113I	GGCGCC	2 cut(s)	351, 716
MlyI	GAGTC	8 cut(s)	1193, 1307, 1451, 1736, 1787, 2553, 2621, 2978
MmeI	TCCRAC	4 cut(s)	63, 183, 448, 1399
MroI	TCCGGA	1 cut(s)	472
MroXI	GAANNNNTTC	1 cut(s)	461
MseI	TTAA	8 cut(s)	932, 1338, 1596, 2066, 2382, 2481, 2838, 3023
MslI	CAYNNNNRTG	3 cut(s)	329, 432, 1325
MspA1I	CMGCKG	5 cut(s)	235, 381, 604, 1571, 1907
MspCI	CTTAAG	1 cut(s)	3022
MspI	CCGG	6 cut(s)	138, 216, 473, 624, 1775, 2001
MspR9I	CCNGG	5 cut(s)	217, 367, 400, 625, 2002
MunI	CAATTG	2 cut(s)	1782, 1875
Mva1269I	GAATGC	1 cut(s)	1482
MvaI	CCWGG	2 cut(s)	367, 400
MvnI	CGCG	1 cut(s)	381
MwoI	GCNNNNNNNGC	7 cut(s)	350, 598, 869, 982, 1016, 2468, 2904
NarI	GGCGCC	2 cut(s)	351, 716
NciI	CCSGG	3 cut(s)	217, 625, 2002
NcoI	CCATGG	1 cut(s)	1077
NmeAIII	GCCGAG	2 cut(s)	72, 618
NmuCI	GTSAC	2 cut(s)	1260, 2885
NspI	RCATGY	6 cut(s)	1268, 1940, 1951, 2207, 2743, 2896
NspV	TTCGAA	1 cut(s)	826
OliI	CACNNNNGTG	1 cut(s)	432
PaeI	GCATGC	2 cut(s)	1940, 2207
PaeR7I	CTCGAG	1 cut(s)	200
PagI	TCATGA	1 cut(s)	688
PceI	AGGCCT	1 cut(s)	743
PciI	ACATGT	1 cut(s)	1264
PciSI	GCTCTTC	1 cut(s)	2193
PcsI	WCGNNNNNNNCGW	4 cut(s)	63, 99, 787, 832
PctI	GAATGC	1 cut(s)	1482
PdmI	GAANNNNTTC	1 cut(s)	461
PflMI	CCANNNNNTGG	1 cut(s)	2462
PfoI	TCCNGGA	1 cut(s)	2000
PleI	GAGTC	8 cut(s)	1193, 1306, 1450, 1735, 1786, 2552, 2620, 2978
PluTI	GGCGCC	2 cut(s)	354, 719
PpsI	GAGTC	8 cut(s)	1193, 1306, 1450, 1735, 1786, 2552, 2620, 2978
PpuMI	RGGWCCY	1 cut(s)	2756
PscI	ACATGT	1 cut(s)	1264
Psp124BI	GAGCTC	4 cut(s)	33, 270, 1884, 2698
Psp5II	RGGWCCY	1 cut(s)	2756
Psp6I	CCWGG	2 cut(s)	365, 398
PspFI	CCCAGC	1 cut(s)	2215
PspGI	CCWGG	2 cut(s)	365, 398
PspPI	GGNCC	4 cut(s)	77, 667, 783, 2756
PspPPI	RGGWCCY	1 cut(s)	2756
PstI	CTGCAG	2 cut(s)	2835, 2909
PstNI	CAGNNNCTG	2 cut(s)	941, 2606
PsuI	RGATCY	2 cut(s)	107, 2339
PvuII	CAGCTG	1 cut(s)	1907
RsaI	GTAC	4 cut(s)	362, 927, 1119, 2956
RsaNI	GTAC	4 cut(s)	361, 926, 1118, 2955
RseI	CAYNNNNRTG	3 cut(s)	329, 432, 1325
SacI	GAGCTC	4 cut(s)	33, 270, 1884, 2698
SacII	CCGCGG	1 cut(s)	382
SapI	GCTCTTC	1 cut(s)	2193
SaqAI	TTAA	8 cut(s)	932, 1338, 1596, 2066, 2382, 2481, 2838, 3023
Sau96I	GGNCC	4 cut(s)	77, 667, 783, 2756
SbfI	CCTGCAGG	1 cut(s)	2835
SchI	GAGTC	8 cut(s)	1193, 1307, 1451, 1736, 1787, 2553, 2621, 2978
ScrFI	CCNGG	5 cut(s)	217, 367, 400, 625, 2002
SdaI	CCTGCAGG	1 cut(s)	2835
SduI	GDGCHC	9 cut(s)	33, 270, 323, 919, 1042, 1884, 2074, 2698, 2900
SfcI	CTRYAG	3 cut(s)	2732, 2831, 2905
SfoI	GGCGCC	2 cut(s)	352, 717
Sfr274I	CTCGAG	1 cut(s)	200
Sfr303I	CCGCGG	1 cut(s)	382
SfuI	TTCGAA	1 cut(s)	826
SgrBI	CCGCGG	1 cut(s)	382
SinI	GGWCC	3 cut(s)	667, 783, 2756
SlaI	CTCGAG	1 cut(s)	200
SmiMI	CAYNNNNRTG	3 cut(s)	329, 432, 1325
SmlI	CTYRAG	7 cut(s)	200, 1098, 1213, 1295, 1796, 2697, 3022
SmoI	CTYRAG	7 cut(s)	200, 1098, 1213, 1295, 1796, 2697, 3022
SphI	GCATGC	2 cut(s)	1940, 2207
Sse8387I	CCTGCAGG	1 cut(s)	2835
SseBI	AGGCCT	1 cut(s)	743
SspDI	GGCGCC	2 cut(s)	350, 715
SspI	AATATT	1 cut(s)	1900
SspMI	CTAG	1 cut(s)	1503
SstI	GAGCTC	4 cut(s)	33, 270, 1884, 2698
StuI	AGGCCT	1 cut(s)	743
StyD4I	CCNGG	5 cut(s)	215, 365, 398, 623, 2000
StyI	CCWWGG	4 cut(s)	339, 345, 1077, 2717
TaiI	ACGT	1 cut(s)	2176
TaqI	TCGA	6 cut(s)	27, 57, 66, 201, 764, 826
TaqII	GACCGA	2 cut(s)	771, 2537
TatI	WGTACW	4 cut(s)	360, 925, 1117, 2954
TauI	GCSGC	4 cut(s)	235, 381, 604, 1574
Tru1I	TTAA	8 cut(s)	932, 1338, 1596, 2066, 2382, 2481, 2838, 3023
Tru9I	TTAA	8 cut(s)	932, 1338, 1596, 2066, 2382, 2481, 2838, 3023
TscAI	CASTG	8 cut(s)	16, 1263, 1819, 2292, 2566, 2782, 2805, 3009
TseFI	GTSAC	2 cut(s)	1260, 2885
Tsp45I	GTSAC	2 cut(s)	1260, 2885
TspDTI	ATGAA	9 cut(s)	705, 1212, 1707, 2001, 2031, 2211, 2240, 2258, 3011
TspGWI	ACGGA	3 cut(s)	762, 2169, 2736
TspRI	CASTG	8 cut(s)	16, 1263, 1819, 2292, 2566, 2782, 2805, 3009
Van91I	CCANNNNNTGG	1 cut(s)	2462
Vha464I	CTTAAG	1 cut(s)	3022
VpaK11BI	GGWCC	3 cut(s)	667, 783, 2756
XagI	CCTNNNNNAGG	1 cut(s)	182
XapI	RAATTY	5 cut(s)	313, 461, 1427, 2373, 2425
XceI	RCATGY	6 cut(s)	1268, 1940, 1951, 2207, 2743, 2896
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1828
XhoI	CTCGAG	1 cut(s)	200
XmnI	GAANNNNTTC	1 cut(s)	461
XspI	CTAG	1 cut(s)	1503

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.