Rorug02G0097300

Protein N-lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009569
Physical Location & Seq
Reverse (-)
8048248 .. 8052853
4606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0097300.1

Sequence Viewer

Length: 2775 bp
ATGACGTCGCCGGCGACCCCGTCGTTTGGTTCCAAGCGATCAAACTACAGCTTACCTGATCATGATCGTCAGCACCACCGCCTTTGCGGCGCAATGCTGCCTATATTTCTCAACGACCTCACTAATCACAACAACCAAGACCTGGTTGAGGTCACACTAGAGCTCAACAACGACGACGATGACATTGTCGTGTGCAGCGTAGCGCCGGTGGGGAACGACACGCCGGAGGAGGAGGAGGAGGAGGAGGAAGATGAGACGGGGACAGGAATGCTAGGAAGGAGCCTCTCCGCTACGTCACGGAGCATTCGCCGCACATTCGGGTGGCTGATCAGATCGTCGTCGTCGAGGACCGCCGCGTCTTCCAACTCCGGCGAACTCGCGGTTCCGCCACGAGAGGCGAGGAGGCTGAAGGCGAAGCTTCAACGGACAAGATCGAGCGCGCAAAGAGCTCTCAACGGCCTGAGGTTCATCAGCAAGACCACCGGACCTAGCGACGCCGAGGAGCTGTGGAAGCAAGTGGAGGCCAGGTTCGAGTCGCTGGCCGTCCACGGATTGCTCGCCAGAGGAGACTTTGGTCGATGCATAGGGATGGCGGACTCGGAGGAGTTTGCGGTGGGGATATTTGACACATTGGCGAGGAGGCGGCGGCAGAAAATTACGAAGATCACGAAGGAAGAACTTCATGATTTTTGGATACAAATCTCGGACCAGAGCTTTGATGCGCGCCTTCAAATTTTCTTCGACATGGCAGATAGCAATGAGGATGGAAGAATCACAAGGGAGGGAGTGCAAGAGCTTATAATGCTGAGTGCTTCAGCAAACAAGCTATCAAAGCTGAAAGAACAAGCTGAAGAATATGCTTCATTGATAATGGAAGAACTAGACCCCGAAAACTTTGGTTATATTGAGTTATGGCAATTGGAAGCACTGCTTTTACAAAGAGACGTATACATAAACTACAGCAGACCATTGAGCGCAGTAAGTGTAGGTTGGAGTCAGAACCTGAATTCCTTCAGGCCCAAGAATCTGCTTCGGAGACTAAATCGTTCACTTCAGTGTCTCATACTAGACAATTTGCAACGCGGTTGGATTTTATTACTGTGGATTGTTGCAATGGCTTCACTTTTTGCCTGGAAATTCTACCAATACAGAAACAAAGCAGCATTTGAAGTCATCGGCTATTGCTTGCCAGTAGCAAAAGGAGCTGCAGAAACTTTGAAGCTTAACATGGCCCTCATCCTTTTACCTGTTTGTCGTAACACATTGACATGGCTTCGATCAACACGAGCTAGGTTCTTCATTCCCTTTGATGACAACATTAATTTCCACAAGATGATTGCATTTGCTATAGCAATTGGAATAATTGTTCATGCTGGGAACCATGTGGCATGTGACTTTCCTCGTCTAGTAAACTCATCTGCGGAAGAATTTGAGCCAATATCCTCTGATTTCCATGGCAAAAACCCGACGTATAAATTCTTATTCACTGGACCAGAAGGCTTGACTGGAATTCTGATGGTGATTTTTATGGCCATCTCATTCACGCTAGCAGCGAGTCACTTTCGAAGGAACATGGTGAGGCTTCCTGCCCCCTTGAACAAACTGACTGGGTTTAATGCGTTCTGGTATTCTCATCATCTGCTAGGTCTGGTCTACGTTTTGCTACTCATCCATGGAAGCTGTTTGTACTTTGTCCACGAGTGGTATCAGAAAACGACATGGATGTACATCTCTGTTCCATTTCTGCTGTATGTAGTAGAGCGCAGTATCCGAACATGTAGATCAAAACACTATTCAGTTAAGATACTAAAGGCTACAATCTTACCCGGAAATGTTATCAGCTTAATCATGTCCAAGCCACCTGGATTTAAGTACAGAAGTGGACAATACATATTTCTACAATGCCCAACAATCTCCCCATTTGAATGGCACCCATTTTCCATCACCTCAGCACCTGGGGATGAGCATCTCAGTGTTCATATCCGAACAGTTGGAGACTGGACGCAAGAATTGAAGCGAGCCATTTCTGAGGGAGATCATTCATTATCTTTAGTTCATCGGACTAGTGTTGCTCAGCCACAAGATATGGATCAAATAAGACTATGCAGCCAACCCAAATTGTTTTTTGATGGTCCATATGGGGCACCGGCACAGGACTACCGCAACTATGATGTCCTACTCCTTGTGGGTCTTGGCATTGGAGCTACCCCTTTTGTAAGCATCCTTAAAGATCTTCTTAACAGCACAAGAACAGCAGAAGAACAACTGGATTCAAACACAGAAACTAGTACACCGTCAGGCAGCCTAAATAGTTTTACATCATCATGTTTAACACCCAATGACAAGAAGAAATCACAGAGGACTACAAATGCTCATTTCTATTGGGTTACCAGAGAACCTGGCTCTTTCGAGTGGTTTAAAGGAGTCATGGATGAAGTGGCAGAAATGGATCACAAAGGTCAGATTGAACTTCACAACTACCTTACGAGTGTCTACGAAGAGGGTGATGCAAGGTCAACTCTAATCACCATGGTCCAAGCTCTCAACCATGCCAAACATGGAGTTGACATCCTATCTGGCACCAGAGTAATGACTCATTTTGCAAGACCTAATTGGAAAGAAGTGTTTACCAGGATAGCTTCCAAGCATCCATTTTCGACAGTAGGGGTATTCTACTGTGGGATGCCAATGTTAGCAAAGGAGCTGAAGATGCTATCACTTGAGCTCAGTCACAAGACATCGACACGCTTTGAATTCCACAAGGAGTACTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

924

Amino Acids

104.95

Weight (kDa)

7.18

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADPH_Ox PF08414 139 - 241 1.3e-38 Respiratory burst NADPH oxidase
Ferric_reduct PF01794 409 - 555 1.8e-10 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 598 - 717 1.1e-30 FAD-binding domain
NAD_binding_6 PF08030 723 - 905 2.1e-48 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 800
AasI GACNNNNNNGTC 1 cut(s) 355
AatII GACGTC 1 cut(s) 8
AccB1I GGYRCC 3 cut(s) 1929, 2143, 2579
AccB7I CCANNNNNTGG 1 cut(s) 142
AccI GTMKAC 3 cut(s) 948, 1653, 2493
AccII CGCG 5 cut(s) 356, 380, 440, 724, 1083
AclWI GGATC 2 cut(s) 2097, 2457
AcoI YGGCCR 2 cut(s) 540, 1530
AcsI RAATTY 7 cut(s) 732, 1006, 1136, 1427, 1475, 1509, 2753
AcuI CTGAAG 6 cut(s) 428, 798, 870, 997, 1037, 2726
AcyI GRCGYC 2 cut(s) 5, 495
AfaI GTAC 5 cut(s) 1688, 1727, 1874, 2290, 2768
AfiI CCNNNNNNNGG 3 cut(s) 26, 142, 148
AflIII ACRYGT 1 cut(s) 1775
AhlI ACTAGT 2 cut(s) 2063, 2285
AjnI CCWGG 7 cut(s) 141, 524, 1130, 1861, 1954, 2398, 2630
AleI CACNNNNGTG 1 cut(s) 1054
AloI GAACNNNNNNTCC 6 cut(s) 512, 544, 992, 1024, 1350, 1382
Alw21I GWGCWC 3 cut(s) 165, 451, 2727
Alw26I GTCTC 6 cut(s) 248, 561, 936, 1030, 1064, 1989
AlwI GGATC 2 cut(s) 2097, 2457
AlwNI CAGNNNCTG 2 cut(s) 1003, 1955
AoxI GGCC 6 cut(s) 457, 522, 540, 1016, 1230, 1530
ApeKI GCWGC 7 cut(s) 97, 195, 1160, 1205, 1550, 2106, 2301
ApoI RAATTY 7 cut(s) 732, 1006, 1136, 1427, 1475, 1509, 2753
ArsI GACNNNNNNTTYG 2 cut(s) 698, 730
AseI ATTAAT 1 cut(s) 1320
Asp700I GAANNNNTTC 2 cut(s) 678, 1010
AspLEI GCGC 8 cut(s) 92, 205, 440, 442, 724, 726, 977, 1764
AspS9I GGNCC 8 cut(s) 348, 485, 706, 1017, 1231, 1490, 2132, 2533
AsuC2I CCSGG 1 cut(s) 1827
AsuHPI GGTGA 5 cut(s) 1531, 1588, 1936, 2516, 2518
AsuII TTCGAA 1 cut(s) 1564
AsuNHI GCTAGC 1 cut(s) 1546
AvaII GGWCC 6 cut(s) 348, 485, 706, 1490, 2132, 2533
AxyI CCTNAGG 1 cut(s) 461
BaeGI GKGCMC 1 cut(s) 2146
BalI TGGCCA 1 cut(s) 1532
BanI GGYRCC 3 cut(s) 1929, 2143, 2579
BanII GRGCYC 3 cut(s) 165, 451, 2727
BauI CACGAG 3 cut(s) 390, 1284, 1697
BbsI GAAGAC 1 cut(s) 351
Bbv12I GWGCWC 3 cut(s) 165, 451, 2727
BbvCI CCTCAGC 1 cut(s) 1948
BbvI GCAGC 7 cut(s) 84, 207, 1172, 1192, 1562, 2118, 2313
BccI CCATC 6 cut(s) 583, 758, 1510, 1541, 1949, 2123
BceAI ACGGC 2 cut(s) 472, 527
BciT130I CCWGG 7 cut(s) 143, 526, 1132, 1863, 1956, 2400, 2632
BciVI GTATCC 2 cut(s) 687, 1778
BclI TGATCA 2 cut(s) 58, 327
BcnI CCSGG 1 cut(s) 1827
BcoDI GTCTC 6 cut(s) 248, 561, 936, 1030, 1064, 1989
BcuI ACTAGT 2 cut(s) 2063, 2285
BfmI CTRYAG 4 cut(s) 46, 958, 1206, 1347
BfoI RGCGCY 1 cut(s) 206
BfuI GTATCC 2 cut(s) 687, 1778
BglI GCCNNNNNGGC 1 cut(s) 87
BglII AGATCT 1 cut(s) 2230
BlpI GCTNAGC 1 cut(s) 2073
BmcAI AGTACT 1 cut(s) 2768
Bme1390I CCNGG 8 cut(s) 143, 526, 1132, 1827, 1863, 1956, 2400, 2632
Bme18I GGWCC 6 cut(s) 348, 485, 706, 1490, 2132, 2533
BmgT120I GGNCC 8 cut(s) 348, 485, 706, 1017, 1231, 1490, 2132, 2533
BmiI GGNNCC 7 cut(s) 31, 281, 384, 1379, 1931, 2145, 2581
BmrFI CCNGG 8 cut(s) 143, 526, 1132, 1827, 1863, 1956, 2400, 2632
BmrI ACTGGG 1 cut(s) 1617
BmsI GCATC 8 cut(s) 569, 709, 1975, 2229, 2497, 2656, 2673, 2700
BmtI GCTAGC 1 cut(s) 1550
BmuI ACTGGG 1 cut(s) 1617
BoxI GACNNNNGTC 1 cut(s) 573
BpiI GAAGAC 1 cut(s) 351
Bpu10I CCTNAGC 1 cut(s) 1948
Bpu1102I GCTNAGC 1 cut(s) 2073
Bpu14I TTCGAA 1 cut(s) 1564
BpuEI CTTGAG 1 cut(s) 2741
BpuMI CCSGG 1 cut(s) 1827
BsaBI GATNNNNATC 5 cut(s) 63, 698, 1727, 1965, 2088
BsaHI GRCGYC 2 cut(s) 5, 495
BsaJI CCNNGG 6 cut(s) 498, 547, 1453, 1672, 1955, 2529
BsaWI WCCGGW 1 cut(s) 482
BsaXI ACNNNNNCTCC 2 cut(s) 512, 542
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 142, 148
Bse118I RCCGGY 3 cut(s) 10, 205, 2146
Bse1I ACTGG 6 cut(s) 1190, 1492, 1510, 1612, 2003, 2271
Bse21I CCTNAGG 1 cut(s) 461
Bse3DI GCAATG 3 cut(s) 99, 763, 1119
Bse8I GATNNNNATC 5 cut(s) 63, 698, 1727, 1965, 2088
BseBI CCWGG 7 cut(s) 143, 526, 1132, 1863, 1956, 2400, 2632
BseDI CCNNGG 6 cut(s) 498, 547, 1453, 1672, 1955, 2529
BseJI GATNNNNATC 5 cut(s) 63, 698, 1727, 1965, 2088
BseLI CCNNNNNNNGG 3 cut(s) 26, 142, 148
BseMI GCAATG 3 cut(s) 99, 763, 1119
BseMII CTCAG 7 cut(s) 452, 797, 1962, 1984, 2019, 2087, 2740
BseNI ACTGG 6 cut(s) 1190, 1492, 1510, 1612, 2003, 2271
BsePI GCGCGC 2 cut(s) 438, 722
BseSI GKGCMC 1 cut(s) 2146
BseXI GCAGC 7 cut(s) 84, 207, 1172, 1192, 1562, 2118, 2313
BseYI CCCAGC 1 cut(s) 1373
BsgI GTGCAG 1 cut(s) 214
Bsh1236I CGCG 5 cut(s) 356, 380, 440, 724, 1083
BshFI GGCC 6 cut(s) 459, 524, 542, 1018, 1232, 1532
BshNI GGYRCC 3 cut(s) 1929, 2143, 2579
BsiHKAI GWGCWC 3 cut(s) 165, 451, 2727
BsiSI CCGG 7 cut(s) 11, 206, 224, 369, 483, 1827, 2147
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 3 cut(s) 26, 142, 148
BsmAI GTCTC 6 cut(s) 248, 561, 936, 1030, 1064, 1989
BsmBI CGTCTC 2 cut(s) 248, 936
BsmFI GGGAC 1 cut(s) 274
BsmI GAATGC 2 cut(s) 273, 303
BsnI GGCC 6 cut(s) 459, 524, 542, 1018, 1232, 1532
Bsp119I TTCGAA 1 cut(s) 1564
Bsp1286I GDGCHC 4 cut(s) 165, 451, 2146, 2727
Bsp1407I TGTACA 1 cut(s) 1725
Bsp1720I GCTNAGC 1 cut(s) 2073
Bsp19I CCATGG 3 cut(s) 1453, 1672, 2529
BspANI GGCC 6 cut(s) 459, 524, 542, 1018, 1232, 1532
BspCNI CTCAG 7 cut(s) 453, 798, 1961, 1983, 2020, 2086, 2739
BspFNI CGCG 5 cut(s) 356, 380, 440, 724, 1083
BspHI TCATGA 2 cut(s) 61, 682
BspLI GGNNCC 7 cut(s) 31, 281, 384, 1379, 1931, 2145, 2581
BspMAI CTGCAG 1 cut(s) 1210
BspOI GCTAGC 1 cut(s) 1550
BspPI GGATC 2 cut(s) 2097, 2457
BspT104I TTCGAA 1 cut(s) 1564
BspT107I GGYRCC 3 cut(s) 1929, 2143, 2579
BsrDI GCAATG 3 cut(s) 99, 763, 1119
BsrFI RCCGGY 3 cut(s) 10, 205, 2146
BsrGI TGTACA 1 cut(s) 1725
BsrI ACTGG 6 cut(s) 1190, 1492, 1510, 1612, 2003, 2271
BssAI RCCGGY 3 cut(s) 10, 205, 2146
BssECI CCNNGG 6 cut(s) 498, 547, 1453, 1672, 1955, 2529
BssHII GCGCGC 2 cut(s) 438, 722
BssNAI GTATAC 1 cut(s) 949
BssNI GRCGYC 2 cut(s) 5, 495
BssSI CACGAG 3 cut(s) 390, 1284, 1697
BssT1I CCWWGG 3 cut(s) 1453, 1672, 2529
Bst1107I GTATAC 1 cut(s) 949
Bst2BI CACGAG 3 cut(s) 390, 1284, 1697
Bst2UI CCWGG 7 cut(s) 143, 526, 1132, 1863, 1956, 2400, 2632
Bst4CI ACNGT 5 cut(s) 1101, 1990, 2295, 2662, 2678
Bst6I CTCTTC 1 cut(s) 2493
BstACI GRCGYC 2 cut(s) 5, 495
BstAUI TGTACA 1 cut(s) 1725
BstBI TTCGAA 1 cut(s) 1564
BstC8I GCNNGC 8 cut(s) 12, 440, 540, 558, 724, 1187, 1548, 2019
BstDEI CTNAG 7 cut(s) 461, 806, 1948, 1970, 2028, 2073, 2726
BstDSI CCRYGG 4 cut(s) 547, 1453, 1672, 2529
BstEII GGTNACC 1 cut(s) 2386
BstENI CCTNNNNNAGG 1 cut(s) 146
BstFNI CGCG 5 cut(s) 356, 380, 440, 724, 1083
BstH2I RGCGCY 1 cut(s) 206
BstHHI GCGC 8 cut(s) 92, 205, 440, 442, 724, 726, 977, 1764
BstMAI GTCTC 6 cut(s) 248, 561, 936, 1030, 1064, 1989
BstMWI GCNNNNNNNGC 8 cut(s) 87, 309, 446, 511, 802, 832, 1202, 2710
BstNI CCWGG 7 cut(s) 143, 526, 1132, 1863, 1956, 2400, 2632
BstNSI RCATGY 2 cut(s) 1392, 1779
BstPAI GACNNNNGTC 1 cut(s) 573
BstPI GGTNACC 1 cut(s) 2386
BstSCI CCNGG 8 cut(s) 141, 524, 1130, 1825, 1861, 1954, 2398, 2630
BstSFI CTRYAG 4 cut(s) 46, 958, 1206, 1347
BstSLI GKGCMC 1 cut(s) 2146
BstUI CGCG 5 cut(s) 356, 380, 440, 724, 1083
BstV1I GCAGC 7 cut(s) 84, 207, 1172, 1192, 1562, 2118, 2313
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 2230
BstXI CCANNNNNNTGG 1 cut(s) 1926
BstYI RGATCY 1 cut(s) 2230
BstZ17I GTATAC 1 cut(s) 949
Bsu36I CCTNAGG 1 cut(s) 461
BsuI GTATCC 2 cut(s) 687, 1778
BsuRI GGCC 6 cut(s) 459, 524, 542, 1018, 1232, 1532
BtgI CCRYGG 4 cut(s) 547, 1453, 1672, 2529
BtsI GCAGTG 1 cut(s) 926
BtsIMutI CAGTG 4 cut(s) 926, 1061, 1485, 1978
Cac8I GCNNGC 8 cut(s) 12, 440, 540, 558, 724, 1187, 1548, 2019
CaiI CAGNNNCTG 2 cut(s) 1003, 1955
CciI TCATGA 2 cut(s) 61, 682
CfoI GCGC 8 cut(s) 92, 205, 440, 442, 724, 726, 977, 1764
Cfr10I RCCGGY 3 cut(s) 10, 205, 2146
Cfr13I GGNCC 8 cut(s) 348, 485, 706, 1017, 1231, 1490, 2132, 2533
CseI GACGC 3 cut(s) 345, 503, 2011
CsiI ACCWGGT 1 cut(s) 141
Csp6I GTAC 5 cut(s) 1687, 1726, 1873, 2289, 2767
CspCI CAANNNNNGTGG 2 cut(s) 65, 100
CviQI GTAC 5 cut(s) 1687, 1726, 1873, 2289, 2767
DdeI CTNAG 7 cut(s) 461, 806, 1948, 1970, 2028, 2073, 2726
DraI TTTAAA 1 cut(s) 2419
DrdI GACNNNNNNGTC 1 cut(s) 355
DseDI GACNNNNNNGTC 1 cut(s) 355
EaeI YGGCCR 2 cut(s) 540, 1530
Eam1104I CTCTTC 1 cut(s) 2493
EarI CTCTTC 1 cut(s) 2493
EciI GGCGGA 2 cut(s) 375, 608
Ecl136II GAGCTC 3 cut(s) 163, 449, 2725
Eco130I CCWWGG 3 cut(s) 1453, 1672, 2529
Eco24I GRGCYC 3 cut(s) 165, 451, 2727
Eco47I GGWCC 6 cut(s) 348, 485, 706, 1490, 2132, 2533
Eco53kI GAGCTC 3 cut(s) 163, 449, 2725
Eco57I CTGAAG 6 cut(s) 428, 798, 870, 997, 1037, 2726
Eco81I CCTNAGG 1 cut(s) 461
Eco91I GGTNACC 1 cut(s) 2386
EcoICRI GAGCTC 3 cut(s) 163, 449, 2725
EcoNI CCTNNNNNAGG 1 cut(s) 146
EcoO65I GGTNACC 1 cut(s) 2386
EcoRI GAATTC 3 cut(s) 1006, 1509, 2753
EcoRII CCWGG 7 cut(s) 141, 524, 1130, 1861, 1954, 2398, 2630
EcoT14I CCWWGG 3 cut(s) 1453, 1672, 2529
EcoT22I ATGCAT 1 cut(s) 584
EcoT38I GRGCYC 3 cut(s) 165, 451, 2727
ErhI CCWWGG 3 cut(s) 1453, 1672, 2529
Esp3I CGTCTC 2 cut(s) 248, 936
FalI AAGNNNNNCTT 5 cut(s) 915, 947, 2220, 2252, 2753
FaqI GGGAC 1 cut(s) 274
FauNDI CATATG 1 cut(s) 2137
FbaI TGATCA 2 cut(s) 58, 327
FblI GTMKAC 3 cut(s) 948, 1653, 2493
FriOI GRGCYC 3 cut(s) 165, 451, 2727
GlaI GCGC 8 cut(s) 91, 204, 439, 441, 723, 725, 976, 1763
GsaI CCCAGC 1 cut(s) 1377
HaeII RGCGCY 1 cut(s) 206
HaeIII GGCC 6 cut(s) 459, 524, 542, 1018, 1232, 1532
HapII CCGG 7 cut(s) 11, 206, 224, 369, 483, 1827, 2147
HgaI GACGC 3 cut(s) 345, 503, 2011
HhaI GCGC 8 cut(s) 92, 205, 440, 442, 724, 726, 977, 1764
Hin1I GRCGYC 2 cut(s) 5, 495
Hin6I GCGC 8 cut(s) 90, 203, 438, 440, 722, 724, 975, 1762
HinP1I GCGC 8 cut(s) 90, 203, 438, 440, 722, 724, 975, 1762
HincII GTYRAC 2 cut(s) 2517, 2566
HindII GTYRAC 2 cut(s) 2517, 2566
HindIII AAGCTT 2 cut(s) 416, 1220
HinfI GANTC 9 cut(s) 533, 596, 771, 994, 1024, 1555, 2270, 2424, 2593
HpaII CCGG 7 cut(s) 11, 206, 224, 369, 483, 1827, 2147
HphI GGTGA 5 cut(s) 1531, 1588, 1936, 2516, 2518
Hpy188III TCNNGA 3 cut(s) 62, 667, 683
Hpy99I CGWCG 9 cut(s) 10, 25, 176, 179, 340, 343, 346, 497, 1471
HpyAV CCTTC 7 cut(s) 270, 403, 664, 737, 1021, 1490, 1560
HpyCH4III ACNGT 5 cut(s) 1101, 1990, 2295, 2662, 2678
HpyCH4IV ACGT 5 cut(s) 5, 293, 945, 1469, 1656
HpyF10VI GCNNNNNNNGC 8 cut(s) 87, 309, 446, 511, 802, 832, 1202, 2710
HpyF3I CTNAG 7 cut(s) 461, 806, 1948, 1970, 2028, 2073, 2726
HpySE526I ACGT 5 cut(s) 5, 293, 945, 1469, 1656
Hsp92I GRCGYC 2 cut(s) 5, 495
HspAI GCGC 8 cut(s) 90, 203, 438, 440, 722, 724, 975, 1762
KroI GCCGGC 1 cut(s) 10
KroNI GCCGGC 1 cut(s) 12
Ksp22I TGATCA 2 cut(s) 58, 327
LmnI GCTCC 6 cut(s) 279, 300, 502, 1202, 2201, 2701
Lsp1109I GCAGC 7 cut(s) 84, 207, 1172, 1192, 1562, 2118, 2313
LweI GCATC 8 cut(s) 569, 709, 1975, 2229, 2497, 2656, 2673, 2700
MabI ACCWGGT 1 cut(s) 141
MaeII ACGT 5 cut(s) 5, 293, 945, 1469, 1656
MaeIII GTNAC 7 cut(s) 151, 294, 1256, 1391, 1556, 2386, 2729
MfeI CAATTG 2 cut(s) 917, 1353
MflI RGATCY 1 cut(s) 2230
MhlI GDGCHC 4 cut(s) 165, 451, 2146, 2727
MlsI TGGCCA 1 cut(s) 1532
MluNI TGGCCA 1 cut(s) 1532
MlyI GAGTC 6 cut(s) 542, 590, 1003, 1564, 2433, 2587
MmeI TCCRAC 4 cut(s) 387, 971, 1067, 1972
Mox20I TGGCCA 1 cut(s) 1532
Mph1103I ATGCAT 1 cut(s) 584
MreI CGCCGGCG 1 cut(s) 10
MroNI GCCGGC 1 cut(s) 10
MroXI GAANNNNTTC 2 cut(s) 678, 1010
MscI TGGCCA 1 cut(s) 1532
MslI CAYNNNNRTG 8 cut(s) 188, 319, 587, 1054, 1267, 1924, 1971, 2323
Msp20I TGGCCA 1 cut(s) 1532
MspI CCGG 7 cut(s) 11, 206, 224, 369, 483, 1827, 2147
MspR9I CCNGG 8 cut(s) 143, 526, 1132, 1827, 1863, 1956, 2400, 2632
MunI CAATTG 2 cut(s) 917, 1353
Mva1269I GAATGC 2 cut(s) 273, 303
MvaI CCWGG 7 cut(s) 143, 526, 1132, 1863, 1956, 2400, 2632
MvnI CGCG 5 cut(s) 356, 380, 440, 724, 1083
MwoI GCNNNNNNNGC 8 cut(s) 87, 309, 446, 511, 802, 832, 1202, 2710
NaeI GCCGGC 1 cut(s) 12
NciI CCSGG 1 cut(s) 1827
NcoI CCATGG 3 cut(s) 1453, 1672, 2529
NdeI CATATG 1 cut(s) 2137
NgoMIV GCCGGC 1 cut(s) 10
NheI GCTAGC 1 cut(s) 1546
NlaIV GGNNCC 7 cut(s) 31, 281, 384, 1379, 1931, 2145, 2581
NmeAIII GCCGAG 1 cut(s) 523
NmuCI GTSAC 5 cut(s) 151, 294, 1391, 1556, 2729
NsiI ATGCAT 1 cut(s) 584
NspI RCATGY 2 cut(s) 1392, 1779
NspV TTCGAA 1 cut(s) 1564
OliI CACNNNNGTG 1 cut(s) 1054
PagI TCATGA 2 cut(s) 61, 682
PauI GCGCGC 2 cut(s) 438, 722
PciI ACATGT 1 cut(s) 1775
PcsI WCGNNNNNNNCGW 6 cut(s) 11, 195, 341, 665, 1282, 1550
PctI GAATGC 2 cut(s) 273, 303
PdiI GCCGGC 1 cut(s) 12
PdmI GAANNNNTTC 2 cut(s) 678, 1010
PfeI GAWTC 3 cut(s) 771, 1024, 2270
PflFI GACNNNGTC 2 cut(s) 19, 185
PflMI CCANNNNNTGG 1 cut(s) 142
PleI GAGTC 6 cut(s) 541, 590, 1002, 1563, 2432, 2587
PpsI GAGTC 6 cut(s) 541, 590, 1002, 1563, 2432, 2587
PscI ACATGT 1 cut(s) 1775
PshAI GACNNNNGTC 1 cut(s) 573
PshBI ATTAAT 1 cut(s) 1320
PsiI TTATAA 1 cut(s) 800
Psp124BI GAGCTC 3 cut(s) 165, 451, 2727
Psp6I CCWGG 7 cut(s) 141, 524, 1130, 1861, 1954, 2398, 2630
PspEI GGTNACC 1 cut(s) 2386
PspFI CCCAGC 1 cut(s) 1373
PspGI CCWGG 7 cut(s) 141, 524, 1130, 1861, 1954, 2398, 2630
PspN4I GGNNCC 7 cut(s) 31, 281, 384, 1379, 1931, 2145, 2581
PspPI GGNCC 8 cut(s) 348, 485, 706, 1017, 1231, 1490, 2132, 2533
PstI CTGCAG 1 cut(s) 1210
PstNI CAGNNNCTG 2 cut(s) 1003, 1955
PsuI RGATCY 1 cut(s) 2230
PsyI GACNNNGTC 2 cut(s) 19, 185
PteI GCGCGC 2 cut(s) 438, 722
RsaI GTAC 5 cut(s) 1688, 1727, 1874, 2290, 2768
RsaNI GTAC 5 cut(s) 1687, 1726, 1873, 2289, 2767
RseI CAYNNNNRTG 8 cut(s) 188, 319, 587, 1054, 1267, 1924, 1971, 2323
SacI GAGCTC 3 cut(s) 165, 451, 2727
Sau96I GGNCC 8 cut(s) 348, 485, 706, 1017, 1231, 1490, 2132, 2533
ScaI AGTACT 1 cut(s) 2768
SchI GAGTC 6 cut(s) 542, 590, 1003, 1564, 2433, 2587
ScrFI CCNGG 8 cut(s) 143, 526, 1132, 1827, 1863, 1956, 2400, 2632
SduI GDGCHC 4 cut(s) 165, 451, 2146, 2727
SexAI ACCWGGT 1 cut(s) 141
SfaNI GCATC 8 cut(s) 569, 709, 1975, 2229, 2497, 2656, 2673, 2700
SfcI CTRYAG 4 cut(s) 46, 958, 1206, 1347
SfuI TTCGAA 1 cut(s) 1564
SgrAI CRCCGGYG 2 cut(s) 10, 205
SinI GGWCC 6 cut(s) 348, 485, 706, 1490, 2132, 2533
SmiMI CAYNNNNRTG 8 cut(s) 188, 319, 587, 1054, 1267, 1924, 1971, 2323
SmlI CTYRAG 1 cut(s) 2720
SmoI CTYRAG 1 cut(s) 2720
SpeI ACTAGT 2 cut(s) 2063, 2285
SstI GAGCTC 3 cut(s) 165, 451, 2727
StyD4I CCNGG 8 cut(s) 141, 524, 1130, 1825, 1861, 1954, 2398, 2630
StyI CCWWGG 3 cut(s) 1453, 1672, 2529
TaaI ACNGT 5 cut(s) 1101, 1990, 2295, 2662, 2678
TaiI ACGT 5 cut(s) 8, 296, 948, 1472, 1659
TatI WGTACW 5 cut(s) 1686, 1725, 1872, 2288, 2766
TauI GCSGC 5 cut(s) 90, 312, 356, 646, 649
TfiI GAWTC 3 cut(s) 771, 1024, 2270
TscAI CASTG 4 cut(s) 933, 1061, 1492, 1978
TseFI GTSAC 5 cut(s) 151, 294, 1391, 1556, 2729
TseI GCWGC 7 cut(s) 97, 195, 1160, 1205, 1550, 2106, 2301
Tsp45I GTSAC 5 cut(s) 151, 294, 1391, 1556, 2729
TspDTI ATGAA 9 cut(s) 457, 671, 852, 1288, 1358, 1967, 2031, 2045, 2448
TspGWI ACGGA 3 cut(s) 313, 439, 564
TspRI CASTG 4 cut(s) 933, 1061, 1492, 1978
Tth111I GACNNNGTC 2 cut(s) 19, 185
Van91I CCANNNNNTGG 1 cut(s) 142
VpaK11BI GGWCC 6 cut(s) 348, 485, 706, 1490, 2132, 2533
VspI ATTAAT 1 cut(s) 1320
XagI CCTNNNNNAGG 1 cut(s) 146
XapI RAATTY 7 cut(s) 732, 1006, 1136, 1427, 1475, 1509, 2753
XceI RCATGY 2 cut(s) 1392, 1779
XcmI CCANNNNNNNNNTGG 1 cut(s) 2555
XmiI GTMKAC 3 cut(s) 948, 1653, 2493
XmnI GAANNNNTTC 2 cut(s) 678, 1010
ZraI GACGTC 1 cut(s) 6
ZrmI AGTACT 1 cut(s) 2768
Zsp2I ATGCAT 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.