FvH4_1g14380

STS14 protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0011513
Physical Location & Seq
Reverse (-)
8026606 .. 8027169
564 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g14380.t1

Sequence Viewer

Length: 564 bp
ATGATGAAGAAAATGACTCGGGTCTTGGTTGTAGCACTCGCCGTGACTATAGCCATATGTTTCACACCGTGTCACGGCCTCTTACTCGATACACCTCGTTATAATTCGACCGCGGAATTCCTGGGAACTCACAACAAAGCAAGAGCCGCAGTTGGGGTTGCCCCTCTCAAATGGAACACTACCCTGGCCGATCACGCTTTCGACATAGCATGGTCCATGATCACTGACGAAATGGGTGAATGCCAAGATTCTTTGGACCCGAAGAGCCTCCATGCAACGGCGTATGACTTCGGTAGCTACAACATGCACTGGGATCATAATCAATCAGTGACGTCGGGCAGGGTGGTGGAGAGGTGGGTGGCGATGAAGAAGTACTACAACTACGCCAGAAACTCGTGCGTGCGCAACTTTAACTGCGCATCGTATACGCAGGTGGTGTGGAGGAAGTCCTTGGAGTTGGGTTGTGATCAGGTAAGATGTTTGGTAAGCGTAGGGATTACTAATGGTGTAACCATCTGTGTTTATAGTCCTCCTGGAAATATTCCAGGGGAGAAACCATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAP

Protein Analysis

188

Amino Acids

20.91

Weight (kDa)

8.4

Isoelectric Point (pI)

30.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 42 - 160 1.4e-13 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 102
AarI CACCTGC 1 cut(s) 421
AatII GACGTC 1 cut(s) 335
Acc16I TGCGCA 2 cut(s) 404, 418
Acc36I ACCTGC 1 cut(s) 421
AccI GTMKAC 1 cut(s) 425
AccII CGCG 1 cut(s) 113
AciI CCGC 3 cut(s) 111, 113, 147
AclWI GGATC 1 cut(s) 321
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 1 cut(s) 116
AcyI GRCGYC 1 cut(s) 332
AdeI CACNNNGTG 1 cut(s) 69
AfaI GTAC 1 cut(s) 374
AfiI CCNNNNNNNGG 3 cut(s) 74, 153, 277
AjnI CCWGG 4 cut(s) 120, 183, 532, 544
AluBI AGCT 1 cut(s) 297
AluI AGCT 1 cut(s) 297
AlwI GGATC 1 cut(s) 321
Ama87I CYCGRG 1 cut(s) 18
AoxI GGCC 2 cut(s) 76, 186
ApoI RAATTY 1 cut(s) 116
AspLEI GCGC 2 cut(s) 405, 419
AspS9I GGNCC 2 cut(s) 213, 256
AsuHPI GGTGA 1 cut(s) 248
AvaI CYCGRG 1 cut(s) 18
AvaII GGWCC 2 cut(s) 213, 256
BarI GAAGNNNNNNTAC 2 cut(s) 359, 391
BauI CACGAG 1 cut(s) 394
BccI CCATC 1 cut(s) 521
BceAI ACGGC 3 cut(s) 26, 91, 294
BciT130I CCWGG 4 cut(s) 122, 185, 534, 546
BclI TGATCA 2 cut(s) 219, 466
BfaI CTAG 1 cut(s) 562
BfmI CTRYAG 1 cut(s) 48
BfuAI ACCTGC 1 cut(s) 421
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
BmcAI AGTACT 1 cut(s) 374
Bme1390I CCNGG 4 cut(s) 122, 185, 534, 546
Bme18I GGWCC 2 cut(s) 213, 256
BmeT110I CYCGRG 1 cut(s) 18
BmgT120I GGNCC 2 cut(s) 213, 256
BmiI GGNNCC 1 cut(s) 258
BmrFI CCNGG 4 cut(s) 122, 185, 534, 546
BmrI ACTGGG 1 cut(s) 319
BmsI GCATC 1 cut(s) 428
BmuI ACTGGG 1 cut(s) 319
BoxI GACNNNNGTC 1 cut(s) 20
BsaBI GATNNNNATC 1 cut(s) 318
BsaHI GRCGYC 1 cut(s) 332
BsaJI CCNNGG 5 cut(s) 111, 121, 183, 450, 545
Bsc4I CCNNNNNNNGG 3 cut(s) 74, 153, 277
Bse1I ACTGG 1 cut(s) 314
Bse8I GATNNNNATC 1 cut(s) 318
BseBI CCWGG 4 cut(s) 122, 185, 534, 546
BseDI CCNNGG 5 cut(s) 111, 121, 183, 450, 545
BseJI GATNNNNATC 1 cut(s) 318
BseLI CCNNNNNNNGG 3 cut(s) 74, 153, 277
BseNI ACTGG 1 cut(s) 314
Bsh1236I CGCG 1 cut(s) 113
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 2 cut(s) 78, 188
BsiEI CGRYCG 1 cut(s) 111
BsiHKCI CYCGRG 1 cut(s) 18
BslI CCNNNNNNNGG 3 cut(s) 74, 153, 277
BsmI GAATGC 1 cut(s) 245
BsnI GGCC 2 cut(s) 78, 188
BsoBI CYCGRG 1 cut(s) 18
Bsp143I GATC 4 cut(s) 190, 219, 313, 466
BspACI CCGC 3 cut(s) 111, 113, 147
BspANI GGCC 2 cut(s) 78, 188
BspFNI CGCG 1 cut(s) 113
BspLI GGNNCC 1 cut(s) 258
BspMI ACCTGC 1 cut(s) 421
BspPI GGATC 1 cut(s) 321
BspQI GCTCTTC 1 cut(s) 257
BsrI ACTGG 1 cut(s) 314
BssECI CCNNGG 5 cut(s) 111, 121, 183, 450, 545
BssMI GATC 4 cut(s) 190, 219, 313, 466
BssNAI GTATAC 1 cut(s) 426
BssNI GRCGYC 1 cut(s) 332
BssSI CACGAG 1 cut(s) 394
BssT1I CCWWGG 1 cut(s) 450
Bst1107I GTATAC 1 cut(s) 426
Bst2BI CACGAG 1 cut(s) 394
Bst2UI CCWGG 4 cut(s) 122, 185, 534, 546
Bst4CI ACNGT 1 cut(s) 69
Bst6I CTCTTC 1 cut(s) 257
BstACI GRCGYC 1 cut(s) 332
BstC8I GCNNGC 1 cut(s) 401
BstDSI CCRYGG 1 cut(s) 111
BstFNI CGCG 1 cut(s) 113
BstHHI GCGC 2 cut(s) 405, 419
BstKTI GATC 4 cut(s) 193, 222, 316, 469
BstMBI GATC 4 cut(s) 190, 219, 313, 466
BstMCI CGRYCG 1 cut(s) 111
BstMWI GCNNNNNNNGC 2 cut(s) 146, 194
BstNI CCWGG 4 cut(s) 122, 185, 534, 546
BstNSI RCATGY 1 cut(s) 307
BstPAI GACNNNNGTC 1 cut(s) 20
BstSCI CCNGG 4 cut(s) 120, 183, 532, 544
BstSFI CTRYAG 1 cut(s) 48
BstUI CGCG 1 cut(s) 113
BstZ17I GTATAC 1 cut(s) 426
BsuRI GGCC 2 cut(s) 78, 188
BtgI CCRYGG 1 cut(s) 111
BtgZI GCGATG 1 cut(s) 377
BtsIMutI CAGTG 3 cut(s) 222, 307, 333
BveI ACCTGC 1 cut(s) 421
Cac8I GCNNGC 1 cut(s) 401
CfoI GCGC 2 cut(s) 405, 419
Cfr13I GGNCC 2 cut(s) 213, 256
Cfr42I CCGCGG 1 cut(s) 114
Csp6I GTAC 1 cut(s) 373
CviAII CATG 4 cut(s) 210, 217, 272, 304
CviJI RGCY 6 cut(s) 53, 78, 146, 188, 267, 297
CviKI_1 RGCY 6 cut(s) 53, 78, 146, 188, 267, 297
CviQI GTAC 1 cut(s) 373
DpnI GATC 4 cut(s) 192, 221, 315, 468
DpnII GATC 4 cut(s) 190, 219, 313, 466
DraIII CACNNNGTG 1 cut(s) 69
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 450
Eco47I GGWCC 2 cut(s) 213, 256
Eco88I CYCGRG 1 cut(s) 18
EcoRI GAATTC 1 cut(s) 116
EcoRII CCWGG 4 cut(s) 120, 183, 532, 544
EcoT14I CCWWGG 1 cut(s) 450
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 4 cut(s) 213, 220, 275, 307
FatI CATG 4 cut(s) 209, 216, 271, 303
FauNDI CATATG 1 cut(s) 56
FbaI TGATCA 2 cut(s) 219, 466
FblI GTMKAC 1 cut(s) 425
Fnu4HI GCNGC 1 cut(s) 147
Fsp4HI GCNGC 1 cut(s) 147
FspBI CTAG 1 cut(s) 562
FspI TGCGCA 2 cut(s) 404, 418
GlaI GCGC 2 cut(s) 404, 418
GluI GCNGC 1 cut(s) 147
HaeIII GGCC 2 cut(s) 78, 188
HhaI GCGC 2 cut(s) 405, 419
Hin1I GRCGYC 1 cut(s) 332
Hin1II CATG 4 cut(s) 213, 220, 275, 307
Hin6I GCGC 2 cut(s) 403, 417
HinP1I GCGC 2 cut(s) 403, 417
HinfI GANTC 2 cut(s) 16, 248
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 1 cut(s) 426
Hpy8I GTNNAC 1 cut(s) 426
Hpy99I CGWCG 1 cut(s) 337
HpyCH4III ACNGT 1 cut(s) 69
HpyCH4IV ACGT 1 cut(s) 332
HpyCH4V TGCA 2 cut(s) 275, 307
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 194
HpySE526I ACGT 1 cut(s) 332
Hsp92I GRCGYC 1 cut(s) 332
Hsp92II CATG 4 cut(s) 213, 220, 275, 307
HspAI GCGC 2 cut(s) 403, 417
Ksp22I TGATCA 2 cut(s) 219, 466
KspI CCGCGG 1 cut(s) 114
Kzo9I GATC 4 cut(s) 190, 219, 313, 466
LguI GCTCTTC 1 cut(s) 257
LweI GCATC 1 cut(s) 428
MaeI CTAG 1 cut(s) 562
MaeII ACGT 1 cut(s) 332
MaeIII GTNAC 4 cut(s) 43, 71, 328, 508
MalI GATC 4 cut(s) 192, 221, 315, 468
MboI GATC 4 cut(s) 190, 219, 313, 466
MboII GAAGA 3 cut(s) 19, 274, 379
MluCI AATT 2 cut(s) 103, 116
MlyI GAGTC 1 cut(s) 10
MnlI CCTC 7 cut(s) 89, 105, 174, 278, 345, 435, 540
MseI TTAA 1 cut(s) 411
MspA1I CMGCKG 1 cut(s) 113
MspR9I CCNGG 4 cut(s) 122, 185, 534, 546
Mva1269I GAATGC 1 cut(s) 245
MvaI CCWGG 4 cut(s) 122, 185, 534, 546
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 2 cut(s) 146, 194
NdeI CATATG 1 cut(s) 56
NdeII GATC 4 cut(s) 190, 219, 313, 466
NlaIII CATG 4 cut(s) 213, 220, 275, 307
NlaIV GGNNCC 1 cut(s) 258
NmuCI GTSAC 3 cut(s) 43, 71, 328
NsbI TGCGCA 2 cut(s) 404, 418
NspI RCATGY 1 cut(s) 307
PaqCI CACCTGC 1 cut(s) 421
PciSI GCTCTTC 1 cut(s) 257
PctI GAATGC 1 cut(s) 245
PfeI GAWTC 1 cut(s) 248
PfoI TCCNGGA 1 cut(s) 532
PkrI GCNGC 1 cut(s) 148
PleI GAGTC 1 cut(s) 10
PpsI GAGTC 1 cut(s) 10
PshAI GACNNNNGTC 1 cut(s) 20
PsiI TTATAA 1 cut(s) 102
Psp6I CCWGG 4 cut(s) 120, 183, 532, 544
PspGI CCWGG 4 cut(s) 120, 183, 532, 544
PspN4I GGNNCC 1 cut(s) 258
PspPI GGNCC 2 cut(s) 213, 256
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
SacII CCGCGG 1 cut(s) 114
SapI GCTCTTC 1 cut(s) 257
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 4 cut(s) 190, 219, 313, 466
Sau96I GGNCC 2 cut(s) 213, 256
ScaI AGTACT 1 cut(s) 374
SchI GAGTC 1 cut(s) 10
ScrFI CCNGG 4 cut(s) 122, 185, 534, 546
SetI ASST 6 cut(s) 97, 299, 335, 356, 435, 474
SfaNI GCATC 1 cut(s) 428
SfcI CTRYAG 1 cut(s) 48
Sfr303I CCGCGG 1 cut(s) 114
SgrBI CCGCGG 1 cut(s) 114
SinI GGWCC 2 cut(s) 213, 256
Sse9I AATT 2 cut(s) 103, 116
SsiI CCGC 3 cut(s) 111, 113, 147
SspI AATATT 1 cut(s) 541
SspMI CTAG 1 cut(s) 562
StyD4I CCNGG 4 cut(s) 120, 183, 532, 544
StyI CCWWGG 1 cut(s) 450
TaaI ACNGT 1 cut(s) 69
TaiI ACGT 1 cut(s) 335
TaqI TCGA 3 cut(s) 87, 107, 201
TasI AATT 2 cut(s) 103, 116
TatI WGTACW 1 cut(s) 372
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 1 cut(s) 248
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 3 cut(s) 229, 314, 333
TseFI GTSAC 3 cut(s) 43, 71, 328
Tsp45I GTSAC 3 cut(s) 43, 71, 328
TspDTI ATGAA 2 cut(s) 20, 380
TspRI CASTG 3 cut(s) 229, 314, 333
VpaK11BI GGWCC 2 cut(s) 213, 256
XapI RAATTY 1 cut(s) 116
XceI RCATGY 1 cut(s) 307
XmiI GTMKAC 1 cut(s) 425
XspI CTAG 1 cut(s) 562
ZraI GACGTC 1 cut(s) 333
ZrmI AGTACT 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.