Rh2AG160100

STS14 protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0011513
Physical Location & Seq
Reverse (-)
15103138 .. 15109614
6477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG160100.1

Sequence Viewer

Length: 687 bp
ATGGATCGGGCCTTGGTTGTAGTAGTAGCCGTGACTGTAGCCGTATGTCTCACGCAGTGTCACGGCTTAGCCATTAAACCTCGTTATATATTTGATGGAATGCCAAAATTAATCATCCAAAGAAAAAATTTTACCCCGGAATTCCTGGAAACTCACAACAAAGCAAGAGCCGCAGTTGGGGTTGCCCCTCTTAAATGGAGCCCTTCCCTGGAAAAGGCCGCAAGCGACGTAGCATGGTCCATGATCAATAACGAAAAGGGTTGCCATTCTTTGGACCCGAGGAGCCTCAATCGAACGTATGACATTGGCAGCTTCAACCTGCACTGGGATCGTTATAGTAGACCATCGCCGCGCGAGGTTGTAGACAAGTGGGTGGCGGAGAAGAAGTACTACAACCACGACAAAAACTCGTGTGTGCGCAACAGTAAGTGCGGTTCGTATACGCAGGTGGTGTGGAGGAATTCCTTGGAGTTGGGGTGTGCTCAGCAAAGATGTCACAAGTGGCTGCAAAGCAATGATTCATTCAAGAAAATGGCCCATCATGCCTCTGTCACACTAGTGCTAGCACTTGCTCTAGCACATGGTGCAGCAGCTCATGTGCCAAATGCAGGAACAGAATTTGTGGATGCTCACAACCAAGCAAGGGCATCAGTTGAGGTCAACCTCTCAGGTGAAGCTCAACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAP

Protein Analysis

228

Amino Acids

25.43

Weight (kDa)

9.18

Isoelectric Point (pI)

31.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 49 - 168 1.4e-18 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 436
Acc16I TGCGCA 1 cut(s) 419
Acc36I ACCTGC 2 cut(s) 327, 436
AccB7I CCANNNNNTGG 1 cut(s) 271
AccI GTMKAC 3 cut(s) 340, 363, 440
AccII CGCG 2 cut(s) 352, 354
AciI CCGC 5 cut(s) 171, 219, 350, 377, 432
AclWI GGATC 2 cut(s) 12, 336
AcsI RAATTY 4 cut(s) 127, 140, 460, 617
AdeI CACNNNGTG 2 cut(s) 57, 584
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 7 cut(s) 177, 208, 214, 271, 325, 608, 643
AgsI TTSAA 2 cut(s) 316, 526
AhlI ACTAGT 1 cut(s) 556
AjnI CCWGG 2 cut(s) 144, 207
AleI CACNNNNGTG 1 cut(s) 557
AluBI AGCT 3 cut(s) 312, 593, 677
AluI AGCT 3 cut(s) 312, 593, 677
Alw21I GWGCWC 1 cut(s) 484
Alw26I GTCTC 1 cut(s) 53
AlwI GGATC 2 cut(s) 12, 336
Ama87I CYCGRG 1 cut(s) 277
AoxI GGCC 3 cut(s) 9, 216, 534
ApeKI GCWGC 4 cut(s) 309, 505, 587, 590
ApoI RAATTY 4 cut(s) 127, 140, 460, 617
AseI ATTAAT 1 cut(s) 110
AspLEI GCGC 2 cut(s) 354, 420
AspS9I GGNCC 4 cut(s) 9, 237, 274, 535
AsuC2I CCSGG 1 cut(s) 137
AsuHPI GGTGA 1 cut(s) 683
AsuNHI GCTAGC 1 cut(s) 562
AvaI CYCGRG 1 cut(s) 277
AvaII GGWCC 2 cut(s) 237, 274
BanII GRGCYC 1 cut(s) 203
BarI GAAGNNNNNNTAC 2 cut(s) 374, 406
BauI CACGAG 1 cut(s) 409
Bbv12I GWGCWC 1 cut(s) 484
BbvI GCAGC 4 cut(s) 321, 492, 599, 602
BccI CCATC 3 cut(s) 89, 352, 546
BceAI ACGGC 3 cut(s) 14, 26, 79
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciT130I CCWGG 2 cut(s) 146, 209
BclI TGATCA 1 cut(s) 243
BcnI CCSGG 1 cut(s) 137
BcoDI GTCTC 1 cut(s) 53
BcuI ACTAGT 1 cut(s) 556
BfaI CTAG 4 cut(s) 557, 563, 575, 685
BfmI CTRYAG 1 cut(s) 36
BfuAI ACCTGC 2 cut(s) 327, 436
BisI GCNGC 7 cut(s) 171, 219, 310, 350, 506, 588, 591
BlpI GCTNAGC 2 cut(s) 67, 483
BlsI GCNGC 7 cut(s) 172, 220, 311, 351, 507, 589, 592
BmcAI AGTACT 1 cut(s) 389
Bme1390I CCNGG 3 cut(s) 137, 146, 209
Bme18I GGWCC 2 cut(s) 237, 274
BmeT110I CYCGRG 1 cut(s) 277
BmgT120I GGNCC 4 cut(s) 9, 237, 274, 535
BmiI GGNNCC 3 cut(s) 200, 276, 284
BmrFI CCNGG 3 cut(s) 137, 146, 209
BmrI ACTGGG 1 cut(s) 334
BmsI GCATC 2 cut(s) 616, 656
BmtI GCTAGC 1 cut(s) 566
BmuI ACTGGG 1 cut(s) 334
Bpu1102I GCTNAGC 2 cut(s) 67, 483
BpuMI CCSGG 1 cut(s) 137
BsaJI CCNNGG 5 cut(s) 12, 135, 207, 278, 465
BsaXI ACNNNNNCTCC 4 cut(s) 371, 401, 461, 491
Bsc4I CCNNNNNNNGG 7 cut(s) 177, 208, 214, 271, 325, 608, 643
Bse1I ACTGG 1 cut(s) 329
Bse3DI GCAATG 1 cut(s) 520
BseBI CCWGG 2 cut(s) 146, 209
BseDI CCNNGG 5 cut(s) 12, 135, 207, 278, 465
BseGI GGATG 2 cut(s) 114, 631
BseLI CCNNNNNNNGG 7 cut(s) 177, 208, 214, 271, 325, 608, 643
BseMI GCAATG 1 cut(s) 520
BseMII CTCAG 2 cut(s) 497, 681
BseNI ACTGG 1 cut(s) 329
BseRI GAGGAG 1 cut(s) 295
BseXI GCAGC 4 cut(s) 321, 492, 599, 602
BsgI GTGCAG 2 cut(s) 305, 606
Bsh1236I CGCG 2 cut(s) 352, 354
BshFI GGCC 3 cut(s) 11, 218, 536
BsiHKAI GWGCWC 1 cut(s) 484
BsiHKCI CYCGRG 1 cut(s) 277
BsiSI CCGG 1 cut(s) 137
BslI CCNNNNNNNGG 7 cut(s) 177, 208, 214, 271, 325, 608, 643
BsmAI GTCTC 1 cut(s) 53
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 3 cut(s) 11, 218, 536
BsoBI CYCGRG 1 cut(s) 277
Bsp1286I GDGCHC 2 cut(s) 203, 484
Bsp143I GATC 3 cut(s) 4, 243, 328
Bsp1720I GCTNAGC 2 cut(s) 67, 483
BspACI CCGC 5 cut(s) 171, 219, 350, 377, 432
BspANI GGCC 3 cut(s) 11, 218, 536
BspCNI CTCAG 2 cut(s) 496, 680
BspFNI CGCG 2 cut(s) 352, 354
BspLI GGNNCC 3 cut(s) 200, 276, 284
BspMI ACCTGC 2 cut(s) 327, 436
BspOI GCTAGC 1 cut(s) 566
BspPI GGATC 2 cut(s) 12, 336
BsrDI GCAATG 1 cut(s) 520
BsrI ACTGG 1 cut(s) 329
BssECI CCNNGG 5 cut(s) 12, 135, 207, 278, 465
BssMI GATC 3 cut(s) 4, 243, 328
BssNAI GTATAC 1 cut(s) 441
BssSI CACGAG 1 cut(s) 409
BssT1I CCWWGG 2 cut(s) 12, 465
Bst1107I GTATAC 1 cut(s) 441
Bst2BI CACGAG 1 cut(s) 409
Bst2UI CCWGG 2 cut(s) 146, 209
Bst4CI ACNGT 2 cut(s) 37, 425
BstAPI GCANNNNNTGC 1 cut(s) 584
BstC8I GCNNGC 2 cut(s) 223, 564
BstDEI CTNAG 3 cut(s) 67, 483, 667
BstENI CCTNNNNNAGG 1 cut(s) 212
BstF5I GGATG 2 cut(s) 114, 631
BstFNI CGCG 2 cut(s) 352, 354
BstHHI GCGC 2 cut(s) 354, 420
BstKTI GATC 3 cut(s) 7, 246, 331
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 3 cut(s) 4, 243, 328
BstMWI GCNNNNNNNGC 3 cut(s) 170, 542, 584
BstNI CCWGG 2 cut(s) 146, 209
BstSCI CCNGG 3 cut(s) 135, 144, 207
BstSFI CTRYAG 1 cut(s) 36
BstUI CGCG 2 cut(s) 352, 354
BstV1I GCAGC 4 cut(s) 321, 492, 599, 602
BstZ17I GTATAC 1 cut(s) 441
BsuRI GGCC 3 cut(s) 11, 218, 536
BtgZI GCGATG 1 cut(s) 330
BtsCI GGATG 2 cut(s) 114, 631
BtsI GCAGTG 1 cut(s) 62
BtsIMutI CAGTG 2 cut(s) 62, 322
BveI ACCTGC 2 cut(s) 327, 436
Cac8I GCNNGC 2 cut(s) 223, 564
CfoI GCGC 2 cut(s) 354, 420
Cfr13I GGNCC 4 cut(s) 9, 237, 274, 535
Csp6I GTAC 1 cut(s) 388
CviAII CATG 5 cut(s) 234, 241, 542, 581, 596
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 3 cut(s) 67, 483, 667
DpnI GATC 3 cut(s) 6, 245, 330
DpnII GATC 3 cut(s) 4, 243, 328
DraIII CACNNNGTG 2 cut(s) 57, 584
EciI GGCGGA 1 cut(s) 392
Eco130I CCWWGG 2 cut(s) 12, 465
Eco24I GRGCYC 1 cut(s) 203
Eco47I GGWCC 2 cut(s) 237, 274
Eco88I CYCGRG 1 cut(s) 277
EcoNI CCTNNNNNAGG 1 cut(s) 212
EcoRI GAATTC 2 cut(s) 140, 460
EcoRII CCWGG 2 cut(s) 144, 207
EcoT14I CCWWGG 2 cut(s) 12, 465
EcoT38I GRGCYC 1 cut(s) 203
ErhI CCWWGG 2 cut(s) 12, 465
FaeI CATG 5 cut(s) 237, 244, 545, 584, 599
FatI CATG 5 cut(s) 233, 240, 541, 580, 595
FbaI TGATCA 1 cut(s) 243
FblI GTMKAC 3 cut(s) 340, 363, 440
Fnu4HI GCNGC 7 cut(s) 171, 219, 310, 350, 506, 588, 591
FokI GGATG 2 cut(s) 101, 638
FriOI GRGCYC 1 cut(s) 203
Fsp4HI GCNGC 7 cut(s) 171, 219, 310, 350, 506, 588, 591
FspBI CTAG 4 cut(s) 557, 563, 575, 685
FspI TGCGCA 1 cut(s) 419
GlaI GCGC 2 cut(s) 353, 419
GluI GCNGC 7 cut(s) 171, 219, 310, 350, 506, 588, 591
HaeIII GGCC 3 cut(s) 11, 218, 536
HapII CCGG 1 cut(s) 137
HhaI GCGC 2 cut(s) 354, 420
Hin1II CATG 5 cut(s) 237, 244, 545, 584, 599
Hin6I GCGC 2 cut(s) 352, 418
HinP1I GCGC 2 cut(s) 352, 418
HincII GTYRAC 1 cut(s) 661
HindII GTYRAC 1 cut(s) 661
HinfI GANTC 1 cut(s) 518
HpaII CCGG 1 cut(s) 137
HphI GGTGA 1 cut(s) 683
Hpy166II GTNNAC 4 cut(s) 341, 364, 441, 661
Hpy188III TCNNGA 1 cut(s) 526
Hpy8I GTNNAC 4 cut(s) 341, 364, 441, 661
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 1 cut(s) 213
HpyCH4III ACNGT 2 cut(s) 37, 425
HpyCH4IV ACGT 2 cut(s) 228, 296
HpyCH4V TGCA 4 cut(s) 322, 508, 587, 608
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 542, 584
HpyF3I CTNAG 3 cut(s) 67, 483, 667
HpySE526I ACGT 2 cut(s) 228, 296
Hsp92II CATG 5 cut(s) 237, 244, 545, 584, 599
HspAI GCGC 2 cut(s) 352, 418
Ksp22I TGATCA 1 cut(s) 243
Kzo9I GATC 3 cut(s) 4, 243, 328
LmnI GCTCC 2 cut(s) 198, 282
Lsp1109I GCAGC 4 cut(s) 321, 492, 599, 602
LweI GCATC 2 cut(s) 616, 656
MaeI CTAG 4 cut(s) 557, 563, 575, 685
MaeII ACGT 2 cut(s) 228, 296
MaeIII GTNAC 4 cut(s) 31, 59, 494, 550
MalI GATC 3 cut(s) 6, 245, 330
MboI GATC 3 cut(s) 4, 243, 328
MboII GAAGA 1 cut(s) 394
MhlI GDGCHC 2 cut(s) 203, 484
MluCI AATT 5 cut(s) 107, 127, 140, 460, 617
MnlI CCTC 9 cut(s) 90, 198, 273, 296, 349, 450, 556, 649, 674
MseI TTAA 3 cut(s) 75, 110, 192
MslI CAYNNNNRTG 1 cut(s) 557
MspI CCGG 1 cut(s) 137
MspR9I CCNGG 3 cut(s) 137, 146, 209
Mva1269I GAATGC 1 cut(s) 105
MvaI CCWGG 2 cut(s) 146, 209
MvnI CGCG 2 cut(s) 352, 354
MwoI GCNNNNNNNGC 3 cut(s) 170, 542, 584
NciI CCSGG 1 cut(s) 137
NdeII GATC 3 cut(s) 4, 243, 328
NheI GCTAGC 1 cut(s) 562
NlaIII CATG 5 cut(s) 237, 244, 545, 584, 599
NlaIV GGNNCC 3 cut(s) 200, 276, 284
NmuCI GTSAC 4 cut(s) 31, 59, 494, 550
NsbI TGCGCA 1 cut(s) 419
OliI CACNNNNGTG 1 cut(s) 557
PaqCI CACCTGC 1 cut(s) 436
PctI GAATGC 1 cut(s) 105
PfeI GAWTC 1 cut(s) 518
PflMI CCANNNNNTGG 1 cut(s) 271
PfoI TCCNGGA 1 cut(s) 144
PkrI GCNGC 7 cut(s) 172, 220, 311, 351, 507, 589, 592
PshBI ATTAAT 1 cut(s) 110
Psp6I CCWGG 2 cut(s) 144, 207
PspGI CCWGG 2 cut(s) 144, 207
PspN4I GGNNCC 3 cut(s) 200, 276, 284
PspPI GGNCC 4 cut(s) 9, 237, 274, 535
PsrI GAACNNNNNNTAC 2 cut(s) 418, 450
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
RseI CAYNNNNRTG 1 cut(s) 557
SaqAI TTAA 3 cut(s) 75, 110, 192
SatI GCNGC 7 cut(s) 171, 219, 310, 350, 506, 588, 591
Sau3AI GATC 3 cut(s) 4, 243, 328
Sau96I GGNCC 4 cut(s) 9, 237, 274, 535
ScaI AGTACT 1 cut(s) 389
ScrFI CCNGG 3 cut(s) 137, 146, 209
SduI GDGCHC 2 cut(s) 203, 484
SfaNI GCATC 2 cut(s) 616, 656
SfcI CTRYAG 1 cut(s) 36
SinI GGWCC 2 cut(s) 237, 274
SmiMI CAYNNNNRTG 1 cut(s) 557
SpeI ACTAGT 1 cut(s) 556
Sse9I AATT 5 cut(s) 107, 127, 140, 460, 617
SsiI CCGC 5 cut(s) 171, 219, 350, 377, 432
SspMI CTAG 4 cut(s) 557, 563, 575, 685
StyD4I CCNGG 3 cut(s) 135, 144, 207
StyI CCWWGG 2 cut(s) 12, 465
TaaI ACNGT 2 cut(s) 37, 425
TaiI ACGT 2 cut(s) 231, 299
TaqI TCGA 1 cut(s) 292
TasI AATT 5 cut(s) 107, 127, 140, 460, 617
TatI WGTACW 1 cut(s) 387
TauI GCSGC 3 cut(s) 173, 221, 352
TfiI GAWTC 1 cut(s) 518
Tru1I TTAA 3 cut(s) 75, 110, 192
Tru9I TTAA 3 cut(s) 75, 110, 192
TscAI CASTG 2 cut(s) 62, 329
TseFI GTSAC 4 cut(s) 31, 59, 494, 550
TseI GCWGC 4 cut(s) 309, 505, 587, 590
Tsp45I GTSAC 4 cut(s) 31, 59, 494, 550
TspDTI ATGAA 1 cut(s) 510
TspRI CASTG 2 cut(s) 62, 329
Van91I CCANNNNNTGG 1 cut(s) 271
VpaK11BI GGWCC 2 cut(s) 237, 274
VspI ATTAAT 1 cut(s) 110
XagI CCTNNNNNAGG 1 cut(s) 212
XapI RAATTY 4 cut(s) 127, 140, 460, 617
XmiI GTMKAC 3 cut(s) 340, 363, 440
XspI CTAG 4 cut(s) 557, 563, 575, 685
ZrmI AGTACT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.