Rroxscaffold_2G00139940

STS14 protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011513
Physical Location & Seq
Forward (+)
78203704 .. 78204288
585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139940.1

Sequence Viewer

Length: 585 bp
ATGGCTCGGGCCTTGGTTGTAGTACTAGCCGTGACTGTAGCCATATGTCTCACTCAGTGTCACGGCTTACCCGTTAGACCTCATTATATAAACTTTTACGGATCAGGACCAAGGCTCATCCCCAGAGCAAATTTATCCCTGGATTTCCTGGAAAGTCACAACAAAGCAAGAGCCGCAGTTGGGGTTGCCCCTCTTAAATGGAGCCCTTCCCTGGAAAAGGCCGCACACGACGTAGCATGGTCCATGATCTATAATGAAAAGGGTTGCCATTCTTATTTGGACCCGATGAGCCTCAATCGAACGTTTGACATTGGCGGCTACAACCAGCACTGGGATCGTTATGGAACACTGCCACCGGGCGAGGTTGTGGACAAGTGGTTGCTGATGAAGAAGTACTACAACTACGCCAATAACTCGTGTAGTGTGCGCAACAATAGGTGCGGTTGGTATACGCAGATGGTGTGGAGGAACTCCTTGGAGTTGGGGTGTGCTCAGGAAAGATGTTTAAAGAACTGGCAAAGTAACGGTATGACTATCTGTCTTTATAGTCCTCGTGGAAATGTTCCAGGGCAGAGACCGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAP

Protein Analysis

194

Amino Acids

22.07

Weight (kDa)

9.23

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 50 - 181 4.1e-17 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 428
AccI GTMKAC 1 cut(s) 449
AciI CCGC 4 cut(s) 174, 222, 315, 441
AclI AACGTT 1 cut(s) 302
AclWI GGATC 2 cut(s) 109, 342
AcsI RAATTY 1 cut(s) 130
AdeI CACNNNGTG 1 cut(s) 57
AfaI GTAC 3 cut(s) 24, 395, 581
AfiI CCNNNNNNNGG 4 cut(s) 180, 211, 217, 331
AhdI GACNNNNNGTC 1 cut(s) 537
AjnI CCWGG 4 cut(s) 138, 147, 210, 565
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 2 cut(s) 53, 568
AlwI GGATC 2 cut(s) 109, 342
Ama87I CYCGRG 1 cut(s) 6
AoxI GGCC 2 cut(s) 9, 219
ApoI RAATTY 1 cut(s) 130
AspLEI GCGC 1 cut(s) 429
AspS9I GGNCC 4 cut(s) 9, 107, 240, 280
AsuC2I CCSGG 1 cut(s) 357
AvaI CYCGRG 1 cut(s) 6
AvaII GGWCC 3 cut(s) 107, 240, 280
BanII GRGCYC 1 cut(s) 206
BarI GAAGNNNNNNTAC 2 cut(s) 380, 412
BauI CACGAG 2 cut(s) 415, 552
Bbv12I GWGCWC 1 cut(s) 493
BccI CCATC 1 cut(s) 451
BceAI ACGGC 2 cut(s) 14, 79
BcgI CGANNNNNNTGC 2 cut(s) 317, 351
BciT130I CCWGG 4 cut(s) 140, 149, 212, 567
BcnI CCSGG 1 cut(s) 357
BcoDI GTCTC 2 cut(s) 53, 568
BfaI CTAG 2 cut(s) 26, 583
BfmI CTRYAG 1 cut(s) 36
BisI GCNGC 3 cut(s) 174, 222, 316
BlsI GCNGC 3 cut(s) 175, 223, 317
BmcAI AGTACT 2 cut(s) 24, 395
Bme1390I CCNGG 5 cut(s) 140, 149, 212, 357, 567
Bme18I GGWCC 3 cut(s) 107, 240, 280
BmeRI GACNNNNNGTC 1 cut(s) 537
BmeT110I CYCGRG 1 cut(s) 6
BmgT120I GGNCC 4 cut(s) 9, 107, 240, 280
BmiI GGNNCC 2 cut(s) 203, 282
BmrFI CCNGG 5 cut(s) 140, 149, 212, 357, 567
BmrI ACTGGG 1 cut(s) 340
BmuI ACTGGG 1 cut(s) 340
Bpu10I CCTNAGC 1 cut(s) 492
BpuMI CCSGG 1 cut(s) 357
BsaI GGTCTC 1 cut(s) 568
BsaJI CCNNGG 6 cut(s) 12, 110, 138, 210, 474, 566
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bsc4I CCNNNNNNNGG 4 cut(s) 180, 211, 217, 331
Bse1I ACTGG 2 cut(s) 335, 518
BseBI CCWGG 4 cut(s) 140, 149, 212, 567
BseDI CCNNGG 6 cut(s) 12, 110, 138, 210, 474, 566
BseGI GGATG 1 cut(s) 117
BseLI CCNNNNNNNGG 4 cut(s) 180, 211, 217, 331
BseMII CTCAG 2 cut(s) 68, 506
BseNI ACTGG 2 cut(s) 335, 518
BshFI GGCC 2 cut(s) 11, 221
BsiHKAI GWGCWC 1 cut(s) 493
BsiHKCI CYCGRG 1 cut(s) 6
BsiSI CCGG 1 cut(s) 356
BslI CCNNNNNNNGG 4 cut(s) 180, 211, 217, 331
BsmAI GTCTC 2 cut(s) 53, 568
BsnI GGCC 2 cut(s) 11, 221
Bso31I GGTCTC 1 cut(s) 568
BsoBI CYCGRG 1 cut(s) 6
Bsp1286I GDGCHC 2 cut(s) 206, 493
Bsp143I GATC 3 cut(s) 101, 246, 334
BspACI CCGC 4 cut(s) 174, 222, 315, 441
BspANI GGCC 2 cut(s) 11, 221
BspCNI CTCAG 2 cut(s) 67, 505
BspLI GGNNCC 2 cut(s) 203, 282
BspPI GGATC 2 cut(s) 109, 342
BspTNI GGTCTC 1 cut(s) 568
BsrI ACTGG 2 cut(s) 335, 518
BssECI CCNNGG 6 cut(s) 12, 110, 138, 210, 474, 566
BssMI GATC 3 cut(s) 101, 246, 334
BssNAI GTATAC 1 cut(s) 450
BssSI CACGAG 2 cut(s) 415, 552
BssT1I CCWWGG 3 cut(s) 12, 110, 474
Bst1107I GTATAC 1 cut(s) 450
Bst2BI CACGAG 2 cut(s) 415, 552
Bst2UI CCWGG 4 cut(s) 140, 149, 212, 567
Bst4CI ACNGT 3 cut(s) 37, 527, 579
BstDEI CTNAG 2 cut(s) 54, 492
BstENI CCTNNNNNAGG 1 cut(s) 215
BstF5I GGATG 1 cut(s) 117
BstHHI GCGC 1 cut(s) 429
BstKTI GATC 3 cut(s) 104, 249, 337
BstMAI GTCTC 2 cut(s) 53, 568
BstMBI GATC 3 cut(s) 101, 246, 334
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstNI CCWGG 4 cut(s) 140, 149, 212, 567
BstSCI CCNGG 5 cut(s) 138, 147, 210, 355, 565
BstSFI CTRYAG 1 cut(s) 36
BstZ17I GTATAC 1 cut(s) 450
BsuRI GGCC 2 cut(s) 11, 221
BtsCI GGATG 1 cut(s) 117
BtsI GCAGTG 1 cut(s) 347
BtsIMutI CAGTG 3 cut(s) 62, 328, 347
CfoI GCGC 1 cut(s) 429
Cfr13I GGNCC 4 cut(s) 9, 107, 240, 280
Csp6I GTAC 3 cut(s) 23, 394, 580
CviAII CATG 2 cut(s) 237, 244
CviQI GTAC 3 cut(s) 23, 394, 580
DdeI CTNAG 2 cut(s) 54, 492
DpnI GATC 3 cut(s) 103, 248, 336
DpnII GATC 3 cut(s) 101, 246, 334
DraI TTTAAA 1 cut(s) 507
DraIII CACNNNGTG 1 cut(s) 57
DriI GACNNNNNGTC 1 cut(s) 537
Eam1105I GACNNNNNGTC 1 cut(s) 537
Eco130I CCWWGG 3 cut(s) 12, 110, 474
Eco24I GRGCYC 1 cut(s) 206
Eco31I GGTCTC 1 cut(s) 568
Eco47I GGWCC 3 cut(s) 107, 240, 280
Eco88I CYCGRG 1 cut(s) 6
EcoNI CCTNNNNNAGG 1 cut(s) 215
EcoRII CCWGG 4 cut(s) 138, 147, 210, 565
EcoT14I CCWWGG 3 cut(s) 12, 110, 474
EcoT38I GRGCYC 1 cut(s) 206
ErhI CCWWGG 3 cut(s) 12, 110, 474
FaeI CATG 2 cut(s) 240, 247
FatI CATG 2 cut(s) 236, 243
FauNDI CATATG 1 cut(s) 44
FblI GTMKAC 1 cut(s) 449
Fnu4HI GCNGC 3 cut(s) 174, 222, 316
FokI GGATG 1 cut(s) 104
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 3 cut(s) 174, 222, 316
FspBI CTAG 2 cut(s) 26, 583
FspI TGCGCA 1 cut(s) 428
GlaI GCGC 1 cut(s) 428
GluI GCNGC 3 cut(s) 174, 222, 316
HaeIII GGCC 2 cut(s) 11, 221
HapII CCGG 1 cut(s) 356
HhaI GCGC 1 cut(s) 429
Hin1II CATG 2 cut(s) 240, 247
Hin6I GCGC 1 cut(s) 427
HinP1I GCGC 1 cut(s) 427
HpaII CCGG 1 cut(s) 356
Hpy166II GTNNAC 2 cut(s) 370, 450
Hpy188III TCNNGA 2 cut(s) 105, 494
Hpy8I GTNNAC 2 cut(s) 370, 450
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 1 cut(s) 216
HpyCH4III ACNGT 3 cut(s) 37, 527, 579
HpyCH4IV ACGT 2 cut(s) 231, 302
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpyF3I CTNAG 2 cut(s) 54, 492
HpySE526I ACGT 2 cut(s) 231, 302
Hsp92II CATG 2 cut(s) 240, 247
HspAI GCGC 1 cut(s) 427
Kzo9I GATC 3 cut(s) 101, 246, 334
LmnI GCTCC 1 cut(s) 201
MaeI CTAG 2 cut(s) 26, 583
MaeII ACGT 2 cut(s) 231, 302
MaeIII GTNAC 4 cut(s) 31, 59, 155, 521
MalI GATC 3 cut(s) 103, 248, 336
MboI GATC 3 cut(s) 101, 246, 334
MboII GAAGA 1 cut(s) 400
MhlI GDGCHC 2 cut(s) 206, 493
MluCI AATT 1 cut(s) 130
MnlI CCTC 6 cut(s) 90, 201, 302, 355, 459, 561
MseI TTAA 2 cut(s) 195, 506
MspI CCGG 1 cut(s) 356
MspR9I CCNGG 5 cut(s) 140, 149, 212, 357, 567
MvaI CCWGG 4 cut(s) 140, 149, 212, 567
MwoI GCNNNNNNNGC 1 cut(s) 173
NciI CCSGG 1 cut(s) 357
NdeI CATATG 1 cut(s) 44
NdeII GATC 3 cut(s) 101, 246, 334
NlaIII CATG 2 cut(s) 240, 247
NlaIV GGNNCC 2 cut(s) 203, 282
NmuCI GTSAC 3 cut(s) 31, 59, 155
NsbI TGCGCA 1 cut(s) 428
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PfoI TCCNGGA 1 cut(s) 147
PkrI GCNGC 3 cut(s) 175, 223, 317
Psp1406I AACGTT 1 cut(s) 302
Psp6I CCWGG 4 cut(s) 138, 147, 210, 565
PspGI CCWGG 4 cut(s) 138, 147, 210, 565
PspN4I GGNNCC 2 cut(s) 203, 282
PspPI GGNCC 4 cut(s) 9, 107, 240, 280
RsaI GTAC 3 cut(s) 24, 395, 581
RsaNI GTAC 3 cut(s) 23, 394, 580
SaqAI TTAA 2 cut(s) 195, 506
SatI GCNGC 3 cut(s) 174, 222, 316
Sau3AI GATC 3 cut(s) 101, 246, 334
Sau96I GGNCC 4 cut(s) 9, 107, 240, 280
ScaI AGTACT 2 cut(s) 24, 395
ScrFI CCNGG 5 cut(s) 140, 149, 212, 357, 567
SduI GDGCHC 2 cut(s) 206, 493
SetI ASST 5 cut(s) 82, 234, 305, 366, 440
SfcI CTRYAG 1 cut(s) 36
SinI GGWCC 3 cut(s) 107, 240, 280
Sse9I AATT 1 cut(s) 130
SsiI CCGC 4 cut(s) 174, 222, 315, 441
SspMI CTAG 2 cut(s) 26, 583
StyD4I CCNGG 5 cut(s) 138, 147, 210, 355, 565
StyI CCWWGG 3 cut(s) 12, 110, 474
TaaI ACNGT 3 cut(s) 37, 527, 579
TaiI ACGT 2 cut(s) 234, 305
TaqI TCGA 1 cut(s) 298
TasI AATT 1 cut(s) 130
TatI WGTACW 2 cut(s) 22, 393
TauI GCSGC 3 cut(s) 176, 224, 318
Tru1I TTAA 2 cut(s) 195, 506
Tru9I TTAA 2 cut(s) 195, 506
TscAI CASTG 3 cut(s) 62, 335, 354
TseFI GTSAC 3 cut(s) 31, 59, 155
Tsp45I GTSAC 3 cut(s) 31, 59, 155
TspDTI ATGAA 2 cut(s) 270, 401
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 3 cut(s) 62, 335, 354
VpaK11BI GGWCC 3 cut(s) 107, 240, 280
XagI CCTNNNNNAGG 1 cut(s) 215
XapI RAATTY 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 449
XspI CTAG 2 cut(s) 26, 583
ZrmI AGTACT 2 cut(s) 24, 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.