FvH4_1g15300
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0018775
Physical Location & Seq
Forward (+)
8677575 .. 8679126
1552 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g15300.t1

Sequence Viewer

Length: 1098 bp
ATGGCTCTAGTAGGGGAGCTTGTTGCCGGAGCTGCTTTAGGAACACTGTTCGCCGAGCTGTACAAGGGGTTCAATGAACTGAGAGACGAAACTAAGCGCTTTGGAGGCCGCCTAGAGATTCTTGACCGCACCCTTGAATCTCTGAAACCTCTGATCTTCAAACAGCTGCTAGAGCTGAACCAGGAACTGGATCTCCCAACTACGGATATCGAAGATCTCGAAAGAAAGATGACGGATGGCAAAGAGCTGGTAATCAGACTGAGTCAGATTCGGAAGAGGGAGTACTTGTTCGTCAGGCGAAGTTACAGTAACCAGCTTGTTGAAGTGGATCGTTCGCTTAAGATACTGTTGGATAGACTCAAGCTTCAGCAAGCAAGCGATGTGAAGAAAATCTTACTCGTGGTGAGAAAAAACACCGCGAAACTCGGCGAAATGGAGAAGCAAAATAAAATGCTGCTGGATATACTACTGAATGTGAGGCGGGAGATTGGGGAGGTAAAGGTGACTTTGGTGACTTTGCAGTCTGCAGTAGAGAGCAGTAAACAATTTCTTGAATTAGAAGCGTCTACTACACGACTGCTGGGTGAACCCGCGGTGCAGCAGCAAGCAAAGAATGTGGAGGAGACCAGGATTTTGGTGACGAGTACAGAGAAGGCGCTGGAACGAGTTAAAGAGAATAGTGTGTTAAAAGTTTTGGCATTGGTAATGCAACGAACTGCGGTGGGAACAATATTGCAGGAGCTGTTTGATGCGCTATATAAAGTGAAGGGAAAATATCCGAAGCTGCTACAATTTCTGCAACCAATGATCGAAGATATGCAGGCAAAATATAACAAGGTCTTGGATCATCCAAAAGAAGAACTAGAGAATTTCAAAATGCTTGTGAAAAAGGCATTGGACCTCTTACGCTTCAAGCTTTGTGCAAGGATCAGCTACAAGAAGCACAAGTATGGCAACCAGCTTGTAATATTGGATAAATCTCTTCAGAAGCTGTTGGACATATTAAAAGTGATGGAATTGAGTAACAGTACCAACGTGACGACCTTGGCTTCTGTCACGAATATGGAGGCAGTAGTTCATAGACTATTAGATGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

366

Amino Acids

41.88

Weight (kDa)

9.34

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 142 3e-21 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018775)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15300
rosa_laevigata RLG00000008404 RLG00000008405 RLG00000017331
rosa_multiflora Rmu_co8367303.1_g000001
rosa_roxburghii Rroxscaffold_2G00138970
rosa_samantha Rh4AG162700 Rh4DG158400
rosa_wichuraiana Rw4G013550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 520
AccB7I CCANNNNNTGG 1 cut(s) 187
AccI GTMKAC 1 cut(s) 566
AccII CGCG 2 cut(s) 419, 593
AciI CCGC 7 cut(s) 109, 127, 417, 481, 591, 593, 719
AclWI GGATC 4 cut(s) 198, 336, 852, 935
AcsI RAATTY 1 cut(s) 868
AcuI CTGAAG 2 cut(s) 350, 968
AfaI GTAC 4 cut(s) 62, 284, 646, 1030
AfeI AGCGCT 1 cut(s) 98
AfiI CCNNNNNNNGG 2 cut(s) 187, 202
AflII CTTAAG 1 cut(s) 338
AgsI TTSAA 7 cut(s) 73, 137, 160, 323, 554, 874, 913
AjnI CCWGG 2 cut(s) 180, 626
AjuI GAANNNNNNNTTGG 2 cut(s) 878, 910
AloI GAACNNNNNNTCC 4 cut(s) 177, 209, 272, 304
Alw26I GTCTC 2 cut(s) 78, 617
AlwI GGATC 4 cut(s) 198, 336, 852, 935
AlwNI CAGNNNCTG 3 cut(s) 187, 742, 991
Aor51HI AGCGCT 1 cut(s) 98
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 6 cut(s) 32, 166, 454, 598, 601, 784
ApoI RAATTY 1 cut(s) 868
ArsI GACNNNNNNTTYG 2 cut(s) 616, 648
AspLEI GCGC 3 cut(s) 99, 658, 754
AspS9I GGNCC 1 cut(s) 898
AsuHPI GGTGA 5 cut(s) 415, 514, 523, 596, 649
AvaII GGWCC 1 cut(s) 898
BarI GAAGNNNNNNTAC 2 cut(s) 266, 298
BauI CACGAG 1 cut(s) 398
BbvI GCAGC 6 cut(s) 19, 153, 441, 610, 613, 771
BccI CCATC 2 cut(s) 230, 1006
BciT130I CCWGG 2 cut(s) 182, 628
BcoDI GTCTC 2 cut(s) 78, 617
BfaI CTAG 4 cut(s) 8, 113, 170, 863
BfmI CTRYAG 1 cut(s) 525
BfoI RGCGCY 2 cut(s) 100, 659
BfrI CTTAAG 1 cut(s) 338
BglII AGATCT 1 cut(s) 214
BisI GCNGC 7 cut(s) 33, 109, 167, 455, 599, 602, 785
BlsI GCNGC 7 cut(s) 34, 110, 168, 456, 600, 603, 786
BmcAI AGTACT 1 cut(s) 284
Bme1390I CCNGG 2 cut(s) 182, 628
Bme18I GGWCC 1 cut(s) 898
BmgT120I GGNCC 1 cut(s) 898
BmrFI CCNGG 2 cut(s) 182, 628
BmsI GCATC 1 cut(s) 739
BpuEI CTTGAG 1 cut(s) 344
BsaI GGTCTC 1 cut(s) 617
BsaJI CCNNGG 2 cut(s) 591, 1044
BsaXI ACNNNNNCTCC 4 cut(s) 177, 207, 272, 302
Bsc4I CCNNNNNNNGG 2 cut(s) 187, 202
Bse1I ACTGG 1 cut(s) 192
BseBI CCWGG 2 cut(s) 182, 628
BseDI CCNNGG 2 cut(s) 591, 1044
BseGI GGATG 2 cut(s) 241, 847
BseLI CCNNNNNNNGG 2 cut(s) 187, 202
BseMII CTCAG 2 cut(s) 71, 251
BseNI ACTGG 1 cut(s) 192
BseRI GAGGAG 1 cut(s) 635
BseXI GCAGC 6 cut(s) 19, 153, 441, 610, 613, 771
BseYI CCCAGC 1 cut(s) 580
BsgI GTGCAG 1 cut(s) 617
Bsh1236I CGCG 2 cut(s) 419, 593
BshFI GGCC 1 cut(s) 108
BsiSI CCGG 1 cut(s) 27
BslI CCNNNNNNNGG 2 cut(s) 187, 202
BsmAI GTCTC 2 cut(s) 78, 617
BsmBI CGTCTC 1 cut(s) 78
BsnI GGCC 1 cut(s) 108
Bso31I GGTCTC 1 cut(s) 617
Bsp1407I TGTACA 1 cut(s) 60
Bsp143I GATC 7 cut(s) 153, 190, 214, 328, 807, 844, 927
BspACI CCGC 7 cut(s) 109, 127, 417, 481, 591, 593, 719
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 2 cut(s) 72, 252
BspFNI CGCG 2 cut(s) 419, 593
BspMAI CTGCAG 1 cut(s) 529
BspPI GGATC 4 cut(s) 198, 336, 852, 935
BspTI CTTAAG 1 cut(s) 338
BspTNI GGTCTC 1 cut(s) 617
BsrGI TGTACA 1 cut(s) 60
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 2 cut(s) 591, 1044
BssMI GATC 7 cut(s) 153, 190, 214, 328, 807, 844, 927
BssSI CACGAG 1 cut(s) 398
BssT1I CCWWGG 1 cut(s) 1044
Bst2BI CACGAG 1 cut(s) 398
Bst2UI CCWGG 2 cut(s) 182, 628
Bst4CI ACNGT 4 cut(s) 48, 308, 348, 1028
Bst6I CTCTTC 2 cut(s) 269, 987
BstAFI CTTAAG 1 cut(s) 338
BstAUI TGTACA 1 cut(s) 60
BstC8I GCNNGC 4 cut(s) 372, 376, 606, 822
BstDEI CTNAG 3 cut(s) 80, 93, 260
BstDSI CCRYGG 1 cut(s) 591
BstF5I GGATG 2 cut(s) 241, 847
BstFNI CGCG 2 cut(s) 419, 593
BstH2I RGCGCY 2 cut(s) 100, 659
BstHHI GCGC 3 cut(s) 99, 658, 754
BstKTI GATC 7 cut(s) 156, 193, 217, 331, 810, 847, 930
BstMAI GTCTC 2 cut(s) 78, 617
BstMBI GATC 7 cut(s) 153, 190, 214, 328, 807, 844, 927
BstMWI GCNNNNNNNGC 3 cut(s) 32, 105, 172
BstNI CCWGG 2 cut(s) 182, 628
BstSCI CCNGG 2 cut(s) 180, 626
BstSFI CTRYAG 1 cut(s) 525
BstUI CGCG 2 cut(s) 419, 593
BstV1I GCAGC 6 cut(s) 19, 153, 441, 610, 613, 771
BstX2I RGATCY 2 cut(s) 190, 214
BstXI CCANNNNNNTGG 1 cut(s) 634
BstYI RGATCY 2 cut(s) 190, 214
BsuRI GGCC 1 cut(s) 108
BtgI CCRYGG 1 cut(s) 591
BtgZI GCGATG 1 cut(s) 393
BtsCI GGATG 2 cut(s) 241, 847
BtsIMutI CAGTG 1 cut(s) 44
Cac8I GCNNGC 4 cut(s) 372, 376, 606, 822
CaiI CAGNNNCTG 3 cut(s) 187, 742, 991
CfoI GCGC 3 cut(s) 99, 658, 754
Cfr13I GGNCC 1 cut(s) 898
Cfr42I CCGCGG 1 cut(s) 594
CseI GACGC 1 cut(s) 552
Csp6I GTAC 4 cut(s) 61, 283, 645, 1029
CspCI CAANNNNNGTGG 2 cut(s) 597, 632
CviQI GTAC 4 cut(s) 61, 283, 645, 1029
DdeI CTNAG 3 cut(s) 80, 93, 260
DpnI GATC 7 cut(s) 155, 192, 216, 330, 809, 846, 929
DpnII GATC 7 cut(s) 153, 190, 214, 328, 807, 844, 927
DrdI GACNNNNNNGTC 1 cut(s) 520
DseDI GACNNNNNNGTC 1 cut(s) 520
Eam1104I CTCTTC 2 cut(s) 269, 987
EarI CTCTTC 2 cut(s) 269, 987
Eco130I CCWWGG 1 cut(s) 1044
Eco31I GGTCTC 1 cut(s) 617
Eco32I GATATC 1 cut(s) 208
Eco47I GGWCC 1 cut(s) 898
Eco47III AGCGCT 1 cut(s) 98
Eco57I CTGAAG 2 cut(s) 350, 968
EcoRII CCWGG 2 cut(s) 180, 626
EcoRV GATATC 1 cut(s) 208
EcoT14I CCWWGG 1 cut(s) 1044
ErhI CCWWGG 1 cut(s) 1044
Esp3I CGTCTC 1 cut(s) 78
FalI AAGNNNNNCTT 2 cut(s) 377, 409
FauI CCCGC 2 cut(s) 474, 598
FblI GTMKAC 1 cut(s) 566
Fnu4HI GCNGC 7 cut(s) 33, 109, 167, 455, 599, 602, 785
FokI GGATG 2 cut(s) 248, 834
Fsp4HI GCNGC 7 cut(s) 33, 109, 167, 455, 599, 602, 785
FspBI CTAG 4 cut(s) 8, 113, 170, 863
GlaI GCGC 3 cut(s) 98, 657, 753
GluI GCNGC 7 cut(s) 33, 109, 167, 455, 599, 602, 785
GsaI CCCAGC 1 cut(s) 584
HaeII RGCGCY 2 cut(s) 100, 659
HaeIII GGCC 1 cut(s) 108
HapII CCGG 1 cut(s) 27
HgaI GACGC 1 cut(s) 552
HhaI GCGC 3 cut(s) 99, 658, 754
Hin6I GCGC 3 cut(s) 97, 656, 752
HinP1I GCGC 3 cut(s) 97, 656, 752
HindIII AAGCTT 2 cut(s) 362, 914
HinfI GANTC 5 cut(s) 118, 137, 262, 268, 357
HpaII CCGG 1 cut(s) 27
HphI GGTGA 5 cut(s) 415, 514, 523, 596, 649
Hpy166II GTNNAC 3 cut(s) 542, 567, 587
Hpy188I TCNGA 7 cut(s) 144, 153, 257, 267, 273, 780, 987
Hpy188III TCNNGA 4 cut(s) 122, 218, 551, 1057
Hpy8I GTNNAC 3 cut(s) 542, 567, 587
HpyAV CCTTC 2 cut(s) 646, 760
HpyCH4III ACNGT 4 cut(s) 48, 308, 348, 1028
HpyCH4IV ACGT 1 cut(s) 1035
HpyCH4V TGCA 8 cut(s) 520, 527, 598, 709, 736, 799, 820, 923
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 105, 172
HpyF3I CTNAG 3 cut(s) 80, 93, 260
HpySE526I ACGT 1 cut(s) 1035
HspAI GCGC 3 cut(s) 97, 656, 752
KspI CCGCGG 1 cut(s) 594
Kzo9I GATC 7 cut(s) 153, 190, 214, 328, 807, 844, 927
LmnI GCTCC 3 cut(s) 16, 29, 739
Lsp1109I GCAGC 6 cut(s) 19, 153, 441, 610, 613, 771
LweI GCATC 1 cut(s) 739
MaeI CTAG 4 cut(s) 8, 113, 170, 863
MaeII ACGT 1 cut(s) 1035
MaeIII GTNAC 8 cut(s) 302, 308, 502, 511, 637, 1022, 1036, 1054
MalI GATC 7 cut(s) 155, 192, 216, 330, 809, 846, 929
MboI GATC 7 cut(s) 153, 190, 214, 328, 807, 844, 927
MboII GAAGA 7 cut(s) 148, 224, 286, 397, 824, 869, 974
MflI RGATCY 2 cut(s) 190, 214
MluCI AATT 5 cut(s) 545, 554, 791, 868, 1016
MlyI GAGTC 2 cut(s) 271, 351
MmeI TCCRAC 2 cut(s) 330, 975
MnlI CCTC 8 cut(s) 98, 159, 270, 471, 487, 613, 911, 1060
MseI TTAA 4 cut(s) 339, 669, 686, 1004
MslI CAYNNNNRTG 2 cut(s) 948, 1061
MspA1I CMGCKG 2 cut(s) 166, 593
MspCI CTTAAG 1 cut(s) 338
MspI CCGG 1 cut(s) 27
MspR9I CCNGG 2 cut(s) 182, 628
MvaI CCWGG 2 cut(s) 182, 628
MvnI CGCG 2 cut(s) 419, 593
MwoI GCNNNNNNNGC 3 cut(s) 32, 105, 172
NdeII GATC 7 cut(s) 153, 190, 214, 328, 807, 844, 927
NmeAIII GCCGAG 2 cut(s) 79, 405
NmuCI GTSAC 5 cut(s) 502, 511, 637, 1036, 1054
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PfeI GAWTC 3 cut(s) 118, 137, 268
PflFI GACNNNGTC 1 cut(s) 261
PflMI CCANNNNNTGG 1 cut(s) 187
PkrI GCNGC 7 cut(s) 34, 110, 168, 456, 600, 603, 786
PleI GAGTC 2 cut(s) 270, 351
PpsI GAGTC 2 cut(s) 270, 351
Psp6I CCWGG 2 cut(s) 180, 626
PspFI CCCAGC 1 cut(s) 580
PspGI CCWGG 2 cut(s) 180, 626
PspPI GGNCC 1 cut(s) 898
PsrI GAACNNNNNNTAC 2 cut(s) 53, 85
PstI CTGCAG 1 cut(s) 529
PstNI CAGNNNCTG 3 cut(s) 187, 742, 991
PsuI RGATCY 2 cut(s) 190, 214
PsyI GACNNNGTC 1 cut(s) 261
PvuII CAGCTG 1 cut(s) 166
RsaI GTAC 4 cut(s) 62, 284, 646, 1030
RsaNI GTAC 4 cut(s) 61, 283, 645, 1029
RseI CAYNNNNRTG 2 cut(s) 948, 1061
SacII CCGCGG 1 cut(s) 594
SaqAI TTAA 4 cut(s) 339, 669, 686, 1004
SatI GCNGC 7 cut(s) 33, 109, 167, 455, 599, 602, 785
Sau3AI GATC 7 cut(s) 153, 190, 214, 328, 807, 844, 927
Sau96I GGNCC 1 cut(s) 898
ScaI AGTACT 1 cut(s) 284
SchI GAGTC 2 cut(s) 271, 351
ScrFI CCNGG 2 cut(s) 182, 628
SfaNI GCATC 1 cut(s) 739
SfcI CTRYAG 1 cut(s) 525
Sfr303I CCGCGG 1 cut(s) 594
SgrBI CCGCGG 1 cut(s) 594
SinI GGWCC 1 cut(s) 898
SmiMI CAYNNNNRTG 2 cut(s) 948, 1061
SmlI CTYRAG 2 cut(s) 338, 359
SmoI CTYRAG 2 cut(s) 338, 359
Sse9I AATT 5 cut(s) 545, 554, 791, 868, 1016
SsiI CCGC 7 cut(s) 109, 127, 417, 481, 591, 593, 719
SspI AATATT 2 cut(s) 732, 969
SspMI CTAG 4 cut(s) 8, 113, 170, 863
StyD4I CCNGG 2 cut(s) 180, 626
StyI CCWWGG 1 cut(s) 1044
TaaI ACNGT 4 cut(s) 48, 308, 348, 1028
TaiI ACGT 1 cut(s) 1038
TaqI TCGA 3 cut(s) 210, 219, 810
TasI AATT 5 cut(s) 545, 554, 791, 868, 1016
TatI WGTACW 3 cut(s) 60, 282, 644
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 3 cut(s) 118, 137, 268
Tru1I TTAA 4 cut(s) 339, 669, 686, 1004
Tru9I TTAA 4 cut(s) 339, 669, 686, 1004
TscAI CASTG 1 cut(s) 51
TseFI GTSAC 5 cut(s) 502, 511, 637, 1036, 1054
TseI GCWGC 6 cut(s) 32, 166, 454, 598, 601, 784
Tsp45I GTSAC 5 cut(s) 502, 511, 637, 1036, 1054
TspDTI ATGAA 2 cut(s) 90, 1067
TspGWI ACGGA 2 cut(s) 218, 248
TspRI CASTG 1 cut(s) 51
Tth111I GACNNNGTC 1 cut(s) 261
Van91I CCANNNNNTGG 1 cut(s) 187
Vha464I CTTAAG 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 898
XapI RAATTY 1 cut(s) 868
XmiI GTMKAC 1 cut(s) 566
XspI CTAG 4 cut(s) 8, 113, 170, 863
ZrmI AGTACT 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.