Rh4DG158400
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0018775
Physical Location & Seq
Forward (+)
31567326 .. 31568643
1318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG158400.1

Sequence Viewer

Length: 1089 bp
ATGGCAATGGTGGGGGATCTTGTTGCAGGTGCTGTTGCAGGTGCTGTTTTAGGAACAGTGTTCGATGAGCTGTACAAGAGGTTCAAGGAAATAAGAGGCGAGACTAAGCGCTTTAGAGGCCACCTAGAGAATCTCAATTGCACTCTTGACTCTCTGAAACCTCTGATCTTCCAGATAGGTGAGCTGAACTTGAAACTGGATCTCCCAAATTCGGAAATTGAAGATCTCATGAGAGAGATGGGGAAGGGCGCAGAGCTGGTAGGCAGACTGAGCCAGATTCGGAAGAGGGACTTGTTAGTCAAGCGAAGTTATAGCAACCAGCTTGTTAAACTGGATCAATCGCTTAAGATACTGTTGGATAGACTCAAGTTTCAGGAAGCAAGGGATGTGAAGGAGATCTATCTCGAGATGAGAAAACAAACGGCAAAACTAGTCGAAATGGAGGAGCTTCTCAAAATGCTTGGGGAGAAACTGAATGTGAGGCAGGAGCCTAGGGATGCGAAGGTGGCGTTGCTTTCTGCAGTAGAGAGCAGTAAACAGCTTGTTGAATTGGCAGCGTCTATTAAACTACTGCTTGGTAAACCCTTGTTGCAGCGGCAAGCAAGAAATGTGGAGGAGACCAGGATTTTGGTGACGAGTACAGAGATGGCGCTGGAGCGAGTTAAAGAGAATGGTGTCTTGAAAGGTTTGGCATCGCTAGTGCAACGGGCTGCTGTGGGTACAATATTACAAGAGCTGTTTGATGCGCTATATAAGATGAAGGGAAGATATTCCAAGCTAGAGTTTCTACAACCACTGATCGAAGATATGCAGGCAAAATATAACAATGTCTTGGATCACCCAAAAGAAGGACTAGAAAATTTCAAAATGCTTGTGGAAACGGCCTTGCGCCTCTCACGGTCCAAGCTTCGTGCAGGGATCAGCTACAAAAAGCACAAGTACGGCAACCAGCTACGTGCAATATTGGATAAATCTCTTCAGAAACTGTTGGACATATTAAAAGTGCAGGAAATGAGTAACACCAACGAGACGAACTTGGTTTCAGTCAGGAATATGGAGACAGTAGTACAAAGATTGTTGGACGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

362

Amino Acids

41.27

Weight (kDa)

9.49

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 11 - 144 2.4e-26 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018775)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15300
rosa_laevigata RLG00000008404 RLG00000008405 RLG00000017331
rosa_multiflora Rmu_co8367303.1_g000001
rosa_roxburghii Rroxscaffold_2G00138970
rosa_samantha Rh4AG162700 Rh4DG158400
rosa_wichuraiana Rw4G013550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 17, 29
AasI GACNNNNNNGTC 1 cut(s) 296
Acc36I ACCTGC 2 cut(s) 17, 29
AciI CCGC 1 cut(s) 595
AclWI GGATC 5 cut(s) 24, 207, 342, 843, 926
AcsI RAATTY 2 cut(s) 208, 859
AcuI CTGAAG 1 cut(s) 962
AfaI GTAC 5 cut(s) 74, 640, 721, 941, 1068
AfeI AGCGCT 1 cut(s) 110
AfiI CCNNNNNNNGG 2 cut(s) 211, 848
AflII CTTAAG 1 cut(s) 344
AgsI TTSAA 6 cut(s) 85, 193, 221, 548, 682, 865
AhlI ACTAGT 1 cut(s) 430
AjnI CCWGG 1 cut(s) 620
Alw26I GTCTC 4 cut(s) 95, 611, 1022, 1052
AlwI GGATC 5 cut(s) 24, 207, 342, 843, 926
AlwNI CAGNNNCTG 3 cut(s) 32, 44, 985
Ama87I CYCGRG 1 cut(s) 404
Aor51HI AGCGCT 1 cut(s) 110
AoxI GGCC 2 cut(s) 118, 882
ApeKI GCWGC 3 cut(s) 554, 592, 710
ApoI RAATTY 2 cut(s) 208, 859
ArsI GACNNNNNNTTYG 2 cut(s) 610, 642
Asp700I GAANNNNTTC 1 cut(s) 769
AspA2I CCTAGG 1 cut(s) 491
AspLEI GCGC 5 cut(s) 111, 251, 652, 748, 891
AspS9I GGNCC 1 cut(s) 900
AsuHPI GGTGA 3 cut(s) 191, 643, 830
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 1 cut(s) 900
AvrII CCTAGG 1 cut(s) 491
BbvI GCAGC 3 cut(s) 566, 604, 697
BccI CCATC 2 cut(s) 232, 640
BceAI ACGGC 3 cut(s) 438, 897, 958
BciT130I CCWGG 1 cut(s) 622
BcoDI GTCTC 4 cut(s) 95, 611, 1022, 1052
BcuI ACTAGT 1 cut(s) 430
BfaI CTAG 6 cut(s) 125, 431, 492, 698, 779, 854
BfmI CTRYAG 1 cut(s) 519
BfoI RGCGCY 2 cut(s) 112, 653
BfrI CTTAAG 1 cut(s) 344
BfuAI ACCTGC 2 cut(s) 17, 29
BglII AGATCT 2 cut(s) 223, 396
BisI GCNGC 4 cut(s) 555, 593, 596, 711
BlnI CCTAGG 1 cut(s) 491
BlsI GCNGC 4 cut(s) 556, 594, 597, 712
Bme1390I CCNGG 1 cut(s) 622
Bme18I GGWCC 1 cut(s) 900
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 1 cut(s) 900
BmiI GGNNCC 1 cut(s) 489
BmrFI CCNGG 1 cut(s) 622
BmsI GCATC 3 cut(s) 487, 701, 733
BpmI CTGGAG 1 cut(s) 674
BpuEI CTTGAG 1 cut(s) 350
BsaAI YACGTR 1 cut(s) 956
BsaI GGTCTC 1 cut(s) 611
BsaJI CCNNGG 1 cut(s) 491
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 848
Bse1I ACTGG 2 cut(s) 201, 336
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 1 cut(s) 622
BseDI CCNNGG 1 cut(s) 491
BseGI GGATG 2 cut(s) 391, 502
BseLI CCNNNNNNNGG 2 cut(s) 211, 848
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 1 cut(s) 260
BseNI ACTGG 2 cut(s) 201, 336
BseRI GAGGAG 2 cut(s) 458, 629
BseXI GCAGC 3 cut(s) 566, 604, 697
BsgI GTGCAG 2 cut(s) 933, 1025
BshFI GGCC 2 cut(s) 120, 884
BsiHKCI CYCGRG 1 cut(s) 404
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 2 cut(s) 211, 848
BsmAI GTCTC 4 cut(s) 95, 611, 1022, 1052
BsmBI CGTCTC 1 cut(s) 1022
BsmFI GGGAC 1 cut(s) 302
BsnI GGCC 2 cut(s) 120, 884
Bso31I GGTCTC 1 cut(s) 611
BsoBI CYCGRG 1 cut(s) 404
Bsp1407I TGTACA 1 cut(s) 72
Bsp143I GATC 9 cut(s) 16, 165, 199, 223, 334, 396, 798, 835, 918
BspACI CCGC 1 cut(s) 595
BspANI GGCC 2 cut(s) 120, 884
BspCNI CTCAG 1 cut(s) 261
BspHI TCATGA 1 cut(s) 228
BspLI GGNNCC 1 cut(s) 489
BspMAI CTGCAG 1 cut(s) 523
BspMI ACCTGC 2 cut(s) 17, 29
BspPI GGATC 5 cut(s) 24, 207, 342, 843, 926
BspTI CTTAAG 1 cut(s) 344
BspTNI GGTCTC 1 cut(s) 611
BsrDI GCAATG 1 cut(s) 12
BsrGI TGTACA 1 cut(s) 72
BsrI ACTGG 2 cut(s) 201, 336
BssECI CCNNGG 1 cut(s) 491
BssMI GATC 9 cut(s) 16, 165, 199, 223, 334, 396, 798, 835, 918
BssT1I CCWWGG 1 cut(s) 491
Bst2UI CCWGG 1 cut(s) 622
Bst4CI ACNGT 5 cut(s) 58, 354, 900, 987, 1063
Bst6I CTCTTC 2 cut(s) 278, 981
BstAFI CTTAAG 1 cut(s) 344
BstAUI TGTACA 1 cut(s) 72
BstBAI YACGTR 1 cut(s) 956
BstC8I GCNNGC 2 cut(s) 600, 813
BstDEI CTNAG 2 cut(s) 105, 269
BstF5I GGATG 2 cut(s) 391, 502
BstH2I RGCGCY 2 cut(s) 112, 653
BstHHI GCGC 5 cut(s) 111, 251, 652, 748, 891
BstKTI GATC 9 cut(s) 19, 168, 202, 226, 337, 399, 801, 838, 921
BstMAI GTCTC 4 cut(s) 95, 611, 1022, 1052
BstMBI GATC 9 cut(s) 16, 165, 199, 223, 334, 396, 798, 835, 918
BstMWI GCNNNNNNNGC 3 cut(s) 117, 270, 506
BstNI CCWGG 1 cut(s) 622
BstSCI CCNGG 1 cut(s) 620
BstSFI CTRYAG 1 cut(s) 519
BstV1I GCAGC 3 cut(s) 566, 604, 697
BstX2I RGATCY 4 cut(s) 16, 199, 223, 396
BstXI CCANNNNNNTGG 1 cut(s) 628
BstYI RGATCY 4 cut(s) 16, 199, 223, 396
BsuRI GGCC 2 cut(s) 120, 884
BtgZI GCGATG 1 cut(s) 678
BtsCI GGATG 2 cut(s) 391, 502
BtsIMutI CAGTG 2 cut(s) 63, 794
BveI ACCTGC 2 cut(s) 17, 29
Cac8I GCNNGC 2 cut(s) 600, 813
CaiI CAGNNNCTG 3 cut(s) 32, 44, 985
CciI TCATGA 1 cut(s) 228
CfoI GCGC 5 cut(s) 111, 251, 652, 748, 891
Cfr13I GGNCC 1 cut(s) 900
CseI GACGC 1 cut(s) 546
Csp6I GTAC 5 cut(s) 73, 639, 720, 940, 1067
CspCI CAANNNNNGTGG 2 cut(s) 591, 626
CviAII CATG 1 cut(s) 229
CviQI GTAC 5 cut(s) 73, 639, 720, 940, 1067
DdeI CTNAG 2 cut(s) 105, 269
DpnI GATC 9 cut(s) 18, 167, 201, 225, 336, 398, 800, 837, 920
DpnII GATC 9 cut(s) 16, 165, 199, 223, 334, 396, 798, 835, 918
DrdI GACNNNNNNGTC 1 cut(s) 296
DseDI GACNNNNNNGTC 1 cut(s) 296
Eam1104I CTCTTC 2 cut(s) 278, 981
EarI CTCTTC 2 cut(s) 278, 981
Eco130I CCWWGG 1 cut(s) 491
Eco31I GGTCTC 1 cut(s) 611
Eco47I GGWCC 1 cut(s) 900
Eco47III AGCGCT 1 cut(s) 110
Eco57I CTGAAG 1 cut(s) 962
Eco88I CYCGRG 1 cut(s) 404
EcoRII CCWGG 1 cut(s) 620
EcoT14I CCWWGG 1 cut(s) 491
ErhI CCWWGG 1 cut(s) 491
Esp3I CGTCTC 1 cut(s) 1022
FaeI CATG 1 cut(s) 232
FaiI YATR 9 cut(s) 230, 312, 751, 753, 809, 822, 995, 1055, 1087
FalI AAGNNNNNCTT 2 cut(s) 275, 307
FaqI GGGAC 1 cut(s) 302
FatI CATG 1 cut(s) 228
Fnu4HI GCNGC 4 cut(s) 555, 593, 596, 711
FokI GGATG 2 cut(s) 398, 509
Fsp4HI GCNGC 4 cut(s) 555, 593, 596, 711
FspBI CTAG 6 cut(s) 125, 431, 492, 698, 779, 854
GlaI GCGC 5 cut(s) 110, 250, 651, 747, 890
GluI GCNGC 4 cut(s) 555, 593, 596, 711
GsuI CTGGAG 1 cut(s) 674
HaeII RGCGCY 2 cut(s) 112, 653
HaeIII GGCC 2 cut(s) 120, 884
HgaI GACGC 1 cut(s) 546
HhaI GCGC 5 cut(s) 111, 251, 652, 748, 891
Hin1II CATG 1 cut(s) 232
Hin6I GCGC 5 cut(s) 109, 249, 650, 746, 889
HinP1I GCGC 5 cut(s) 109, 249, 650, 746, 889
HindIII AAGCTT 1 cut(s) 905
HinfI GANTC 4 cut(s) 130, 149, 277, 363
HphI GGTGA 3 cut(s) 191, 643, 830
Hpy166II GTNNAC 2 cut(s) 536, 581
Hpy188I TCNGA 5 cut(s) 156, 165, 214, 282, 981
Hpy188III TCNNGA 8 cut(s) 146, 172, 229, 374, 404, 406, 679, 1048
Hpy8I GTNNAC 2 cut(s) 536, 581
HpyAV CCTTC 5 cut(s) 238, 385, 496, 754, 842
HpyCH4III ACNGT 5 cut(s) 58, 354, 900, 987, 1063
HpyCH4IV ACGT 2 cut(s) 955, 1083
HpyF10VI GCNNNNNNNGC 3 cut(s) 117, 270, 506
HpyF3I CTNAG 2 cut(s) 105, 269
HpySE526I ACGT 2 cut(s) 955, 1083
Hsp92II CATG 1 cut(s) 232
HspAI GCGC 5 cut(s) 109, 249, 650, 746, 889
Kzo9I GATC 9 cut(s) 16, 165, 199, 223, 334, 396, 798, 835, 918
LmnI GCTCC 3 cut(s) 445, 487, 655
Lsp1109I GCAGC 3 cut(s) 566, 604, 697
LweI GCATC 3 cut(s) 487, 701, 733
MaeI CTAG 6 cut(s) 125, 431, 492, 698, 779, 854
MaeII ACGT 2 cut(s) 955, 1083
MaeIII GTNAC 2 cut(s) 631, 1016
MalI GATC 9 cut(s) 18, 167, 201, 225, 336, 398, 800, 837, 920
MboI GATC 9 cut(s) 16, 165, 199, 223, 334, 396, 798, 835, 918
MboII GAAGA 6 cut(s) 160, 233, 295, 777, 815, 968
MfeI CAATTG 1 cut(s) 136
MflI RGATCY 4 cut(s) 16, 199, 223, 396
MluCI AATT 5 cut(s) 136, 208, 216, 548, 859
MlyI GAGTC 2 cut(s) 143, 357
MmeI TCCRAC 3 cut(s) 336, 969, 1059
MnlI CCTC 9 cut(s) 72, 89, 110, 171, 279, 436, 474, 607, 902
MroXI GAANNNNTTC 1 cut(s) 769
MseI TTAA 5 cut(s) 327, 345, 564, 663, 998
MspA1I CMGCKG 1 cut(s) 595
MspCI CTTAAG 1 cut(s) 344
MspR9I CCNGG 1 cut(s) 622
MunI CAATTG 1 cut(s) 136
MvaI CCWGG 1 cut(s) 622
MwoI GCNNNNNNNGC 3 cut(s) 117, 270, 506
NdeII GATC 9 cut(s) 16, 165, 199, 223, 334, 396, 798, 835, 918
NlaIII CATG 1 cut(s) 232
NlaIV GGNNCC 1 cut(s) 489
NmuCI GTSAC 1 cut(s) 631
PaeR7I CTCGAG 1 cut(s) 404
PagI TCATGA 1 cut(s) 228
PaqCI CACCTGC 2 cut(s) 17, 29
PdmI GAANNNNTTC 1 cut(s) 769
PfeI GAWTC 2 cut(s) 130, 277
PkrI GCNGC 4 cut(s) 556, 594, 597, 712
PleI GAGTC 2 cut(s) 143, 357
PpsI GAGTC 2 cut(s) 143, 357
Ppu21I YACGTR 1 cut(s) 956
Psp6I CCWGG 1 cut(s) 620
PspGI CCWGG 1 cut(s) 620
PspN4I GGNNCC 1 cut(s) 489
PspPI GGNCC 1 cut(s) 900
PsrI GAACNNNNNNTAC 2 cut(s) 65, 97
PstI CTGCAG 1 cut(s) 523
PstNI CAGNNNCTG 3 cut(s) 32, 44, 985
PsuI RGATCY 4 cut(s) 16, 199, 223, 396
RsaI GTAC 5 cut(s) 74, 640, 721, 941, 1068
RsaNI GTAC 5 cut(s) 73, 639, 720, 940, 1067
SaqAI TTAA 5 cut(s) 327, 345, 564, 663, 998
SatI GCNGC 4 cut(s) 555, 593, 596, 711
Sau3AI GATC 9 cut(s) 16, 165, 199, 223, 334, 396, 798, 835, 918
Sau96I GGNCC 1 cut(s) 900
SchI GAGTC 2 cut(s) 143, 357
ScrFI CCNGG 1 cut(s) 622
SfaNI GCATC 3 cut(s) 487, 701, 733
SfcI CTRYAG 1 cut(s) 519
Sfr274I CTCGAG 1 cut(s) 404
SinI GGWCC 1 cut(s) 900
SlaI CTCGAG 1 cut(s) 404
SmlI CTYRAG 3 cut(s) 344, 365, 404
SmoI CTYRAG 3 cut(s) 344, 365, 404
SpeI ACTAGT 1 cut(s) 430
Sse9I AATT 5 cut(s) 136, 208, 216, 548, 859
SsiI CCGC 1 cut(s) 595
SspI AATATT 2 cut(s) 726, 963
SspMI CTAG 6 cut(s) 125, 431, 492, 698, 779, 854
StyD4I CCNGG 1 cut(s) 620
StyI CCWWGG 1 cut(s) 491
TaaI ACNGT 5 cut(s) 58, 354, 900, 987, 1063
TaiI ACGT 2 cut(s) 958, 1086
TaqI TCGA 4 cut(s) 63, 405, 435, 801
TasI AATT 5 cut(s) 136, 208, 216, 548, 859
TatI WGTACW 3 cut(s) 72, 638, 1066
TauI GCSGC 1 cut(s) 598
TfiI GAWTC 2 cut(s) 130, 277
Tru1I TTAA 5 cut(s) 327, 345, 564, 663, 998
Tru9I TTAA 5 cut(s) 327, 345, 564, 663, 998
TscAI CASTG 2 cut(s) 63, 801
TseFI GTSAC 1 cut(s) 631
TseI GCWGC 3 cut(s) 554, 592, 710
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 1 cut(s) 773
TspRI CASTG 2 cut(s) 63, 801
Vha464I CTTAAG 1 cut(s) 344
VpaK11BI GGWCC 1 cut(s) 900
XapI RAATTY 2 cut(s) 208, 859
XhoI CTCGAG 1 cut(s) 404
XmaJI CCTAGG 1 cut(s) 491
XmnI GAANNNNTTC 1 cut(s) 769
XspI CTAG 6 cut(s) 125, 431, 492, 698, 779, 854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.