RLG00000017331
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018775
Physical Location & Seq
Forward (+)
15385938 .. 15387005
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017331

Sequence Viewer

Length: 843 bp
ATGGGGAAGGGCGCTGAGCTGGTAGGCAGACTGAGCCAGATTGGGAAAAGGGAGTACTTGTTAGTCAAGCGAAGTTATAGTAACCAGTTTGTTGAACTGGACCGATCGCTTAAGATACTGTTGGATAGACTCAAGTTTCAGGAAGCAAGGGATGTGAAGCAGATCTTACTCGAGATGAGGAAACAAACGGCTAAACTAGTCGAAATGGAGGAGCTTCTCAAAATGCTGGTGGAGAAACTGAATGTGAGGCAGGAGCCTAGGGATGCGAAAGTGGCGTTGCTTTCTGCAGTAGAGAGCAGTAAACAACTTGTTGAATTGGCAGCGTCTACTAAACTACTGCTTGGTAAACCCGCGGTGCAGCGGCAAGCAAGAAATGTAGAGGAGACCAGGATTTTGGTGACGAGTACAGAGATGGCGCTGGAGCGAGTTAAAGAGAATAGTGTCTTGAAAGGTTTGGCATCGCTAGTGCAACGGACTGCTGTGGGTACAAAATTACAAGAGCTGTTTGATGCGCTATATAAGATGAAGGGAAAATATTCCAAGCTAGAGTTTCTAGAACCACTGATCGAAGATATGCAGGCAAAATATAACAATGTCTTGGATCACCCAAAAGCAGAACTAGAAAATTTCAAAATGCTTGTGGAAAAGGCCTTGCGCCTCTTAAGGTCCAAGCTTTGTGCATCGATCAGCTACAAAAAGCACAAGTACGGCAACCAGCTACTTGCAATATTGGAAAAATCTGTTCAGAAACTGTTGGACATATTAAAAGTGCAGGAATTGAGTAACACCAACGAGACGAGCTTGGTTTCAGTCAGGAATATGGAGACAGTAGTACAAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

281

Amino Acids

32.05

Weight (kDa)

9.65

Isoelectric Point (pI)

31.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018775)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15300
rosa_laevigata RLG00000008404 RLG00000008405 RLG00000017331
rosa_multiflora Rmu_co8367303.1_g000001
rosa_roxburghii Rroxscaffold_2G00138970
rosa_samantha Rh4AG162700 Rh4DG158400
rosa_wichuraiana Rw4G013550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 326
AccII CGCG 1 cut(s) 353
AciI CCGC 3 cut(s) 351, 353, 361
AclWI GGATC 1 cut(s) 609
AcsI RAATTY 1 cut(s) 625
AfaI GTAC 5 cut(s) 56, 406, 487, 707, 834
AflII CTTAAG 2 cut(s) 110, 661
AgsI TTSAA 4 cut(s) 95, 314, 448, 631
AhlI ACTAGT 1 cut(s) 196
AjnI CCWGG 1 cut(s) 386
AluBI AGCT 8 cut(s) 19, 214, 502, 544, 673, 690, 718, 801
AluI AGCT 8 cut(s) 19, 214, 502, 544, 673, 690, 718, 801
Alw26I GTCTC 3 cut(s) 377, 788, 818
AlwI GGATC 1 cut(s) 609
AlwNI CAGNNNCTG 1 cut(s) 751
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 1 cut(s) 648
ApeKI GCWGC 2 cut(s) 320, 358
ApoI RAATTY 1 cut(s) 625
ArsI GACNNNNNNTTYG 2 cut(s) 376, 408
Asp700I GAANNNNTTC 1 cut(s) 535
AspA2I CCTAGG 1 cut(s) 257
AspLEI GCGC 4 cut(s) 14, 418, 514, 657
AspS9I GGNCC 2 cut(s) 100, 666
AsuHPI GGTGA 2 cut(s) 409, 596
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 2 cut(s) 100, 666
AvrII CCTAGG 1 cut(s) 257
BbvI GCAGC 2 cut(s) 332, 370
BccI CCATC 1 cut(s) 406
BceAI ACGGC 2 cut(s) 204, 724
BciT130I CCWGG 1 cut(s) 388
BcoDI GTCTC 3 cut(s) 377, 788, 818
BcuI ACTAGT 1 cut(s) 196
BfaI CTAG 6 cut(s) 197, 258, 464, 545, 554, 620
BfmI CTRYAG 1 cut(s) 285
BfoI RGCGCY 2 cut(s) 15, 419
BfrI CTTAAG 2 cut(s) 110, 661
BglII AGATCT 1 cut(s) 162
BisI GCNGC 3 cut(s) 321, 359, 362
BlnI CCTAGG 1 cut(s) 257
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 3 cut(s) 322, 360, 363
BmcAI AGTACT 1 cut(s) 56
Bme1390I CCNGG 1 cut(s) 388
Bme18I GGWCC 2 cut(s) 100, 666
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 2 cut(s) 100, 666
BmiI GGNNCC 1 cut(s) 255
BmrFI CCNGG 1 cut(s) 388
BmsI GCATC 4 cut(s) 253, 467, 499, 689
BpmI CTGGAG 1 cut(s) 440
Bpu1102I GCTNAGC 1 cut(s) 15
BpuEI CTTGAG 1 cut(s) 116
Bsa29I ATCGAT 1 cut(s) 683
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 2 cut(s) 257, 351
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bse1I ACTGG 2 cut(s) 85, 102
BseBI CCWGG 1 cut(s) 388
BseCI ATCGAT 1 cut(s) 683
BseDI CCNNGG 2 cut(s) 257, 351
BseGI GGATG 2 cut(s) 157, 268
BseMII CTCAG 2 cut(s) 6, 23
BseNI ACTGG 2 cut(s) 85, 102
BseRI GAGGAG 2 cut(s) 224, 395
BseXI GCAGC 2 cut(s) 332, 370
BsgI GTGCAG 2 cut(s) 377, 791
Bsh1236I CGCG 1 cut(s) 353
Bsh1285I CGRYCG 1 cut(s) 107
BshFI GGCC 1 cut(s) 650
BshVI ATCGAT 1 cut(s) 683
BsiEI CGRYCG 1 cut(s) 107
BsiHKCI CYCGRG 1 cut(s) 170
BsmAI GTCTC 3 cut(s) 377, 788, 818
BsmBI CGTCTC 1 cut(s) 788
BsnI GGCC 1 cut(s) 650
Bso31I GGTCTC 1 cut(s) 377
BsoBI CYCGRG 1 cut(s) 170
Bsp143I GATC 5 cut(s) 104, 162, 564, 601, 684
Bsp1720I GCTNAGC 1 cut(s) 15
BspACI CCGC 3 cut(s) 351, 353, 361
BspANI GGCC 1 cut(s) 650
BspCNI CTCAG 2 cut(s) 7, 24
BspDI ATCGAT 1 cut(s) 683
BspFNI CGCG 1 cut(s) 353
BspLI GGNNCC 1 cut(s) 255
BspMAI CTGCAG 1 cut(s) 289
BspPI GGATC 1 cut(s) 609
BspTI CTTAAG 2 cut(s) 110, 661
BspTNI GGTCTC 1 cut(s) 377
BsrI ACTGG 2 cut(s) 85, 102
BssECI CCNNGG 2 cut(s) 257, 351
BssMI GATC 5 cut(s) 104, 162, 564, 601, 684
BssT1I CCWWGG 1 cut(s) 257
Bst2UI CCWGG 1 cut(s) 388
Bst4CI ACNGT 3 cut(s) 120, 753, 829
BstAFI CTTAAG 2 cut(s) 110, 661
BstC8I GCNNGC 2 cut(s) 366, 579
BstDEI CTNAG 2 cut(s) 15, 32
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 2 cut(s) 157, 268
BstFNI CGCG 1 cut(s) 353
BstH2I RGCGCY 2 cut(s) 15, 419
BstHHI GCGC 4 cut(s) 14, 418, 514, 657
BstKTI GATC 5 cut(s) 107, 165, 567, 604, 687
BstMAI GTCTC 3 cut(s) 377, 788, 818
BstMBI GATC 5 cut(s) 104, 162, 564, 601, 684
BstMCI CGRYCG 1 cut(s) 107
BstMWI GCNNNNNNNGC 2 cut(s) 33, 272
BstNI CCWGG 1 cut(s) 388
BstSCI CCNGG 1 cut(s) 386
BstSFI CTRYAG 1 cut(s) 285
BstUI CGCG 1 cut(s) 353
BstV1I GCAGC 2 cut(s) 332, 370
BstX2I RGATCY 1 cut(s) 162
BstXI CCANNNNNNTGG 1 cut(s) 394
BstYI RGATCY 1 cut(s) 162
Bsu15I ATCGAT 1 cut(s) 683
BsuRI GGCC 1 cut(s) 650
BsuTUI ATCGAT 1 cut(s) 683
BtgI CCRYGG 1 cut(s) 351
BtgZI GCGATG 1 cut(s) 444
BtsCI GGATG 2 cut(s) 157, 268
BtsIMutI CAGTG 1 cut(s) 560
Cac8I GCNNGC 2 cut(s) 366, 579
CaiI CAGNNNCTG 1 cut(s) 751
CfoI GCGC 4 cut(s) 14, 418, 514, 657
Cfr13I GGNCC 2 cut(s) 100, 666
Cfr42I CCGCGG 1 cut(s) 354
ClaI ATCGAT 1 cut(s) 683
CseI GACGC 1 cut(s) 312
Csp6I GTAC 5 cut(s) 55, 405, 486, 706, 833
CviQI GTAC 5 cut(s) 55, 405, 486, 706, 833
DdeI CTNAG 2 cut(s) 15, 32
DpnI GATC 5 cut(s) 106, 164, 566, 603, 686
DpnII GATC 5 cut(s) 104, 162, 564, 601, 684
Eco130I CCWWGG 1 cut(s) 257
Eco147I AGGCCT 1 cut(s) 650
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 2 cut(s) 100, 666
Eco88I CYCGRG 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 386
EcoT14I CCWWGG 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 257
Esp3I CGTCTC 1 cut(s) 788
FaiI YATR 7 cut(s) 78, 517, 519, 575, 588, 761, 821
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FauI CCCGC 1 cut(s) 358
FblI GTMKAC 1 cut(s) 326
Fnu4HI GCNGC 3 cut(s) 321, 359, 362
FokI GGATG 2 cut(s) 164, 275
Fsp4HI GCNGC 3 cut(s) 321, 359, 362
FspBI CTAG 6 cut(s) 197, 258, 464, 545, 554, 620
GlaI GCGC 4 cut(s) 13, 417, 513, 656
GluI GCNGC 3 cut(s) 321, 359, 362
GsuI CTGGAG 1 cut(s) 440
HaeII RGCGCY 2 cut(s) 15, 419
HaeIII GGCC 1 cut(s) 650
HgaI GACGC 1 cut(s) 312
HhaI GCGC 4 cut(s) 14, 418, 514, 657
Hin6I GCGC 4 cut(s) 12, 416, 512, 655
HinP1I GCGC 4 cut(s) 12, 416, 512, 655
HindIII AAGCTT 1 cut(s) 671
HinfI GANTC 1 cut(s) 129
HphI GGTGA 2 cut(s) 409, 596
Hpy166II GTNNAC 3 cut(s) 302, 327, 347
Hpy188I TCNGA 1 cut(s) 747
Hpy188III TCNNGA 5 cut(s) 140, 172, 445, 554, 814
Hpy8I GTNNAC 3 cut(s) 302, 327, 347
HpyAV CCTTC 1 cut(s) 520
HpyCH4III ACNGT 3 cut(s) 120, 753, 829
HpyCH4V TGCA 7 cut(s) 287, 358, 469, 577, 680, 725, 772
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 272
HpyF3I CTNAG 2 cut(s) 15, 32
HspAI GCGC 4 cut(s) 12, 416, 512, 655
KspI CCGCGG 1 cut(s) 354
Kzo9I GATC 5 cut(s) 104, 162, 564, 601, 684
LmnI GCTCC 3 cut(s) 211, 253, 421
Lsp1109I GCAGC 2 cut(s) 332, 370
LweI GCATC 4 cut(s) 253, 467, 499, 689
MaeI CTAG 6 cut(s) 197, 258, 464, 545, 554, 620
MaeIII GTNAC 3 cut(s) 80, 397, 782
MalI GATC 5 cut(s) 106, 164, 566, 603, 686
MboI GATC 5 cut(s) 104, 162, 564, 601, 684
MboII GAAGA 1 cut(s) 581
MflI RGATCY 1 cut(s) 162
MluCI AATT 4 cut(s) 314, 491, 625, 776
MlyI GAGTC 1 cut(s) 123
MmeI TCCRAC 2 cut(s) 102, 735
MnlI CCTC 5 cut(s) 171, 202, 240, 373, 668
MroXI GAANNNNTTC 1 cut(s) 535
MseI TTAA 4 cut(s) 111, 429, 662, 764
MspA1I CMGCKG 2 cut(s) 353, 361
MspCI CTTAAG 2 cut(s) 110, 661
MspR9I CCNGG 1 cut(s) 388
MvaI CCWGG 1 cut(s) 388
MvnI CGCG 1 cut(s) 353
MwoI GCNNNNNNNGC 2 cut(s) 33, 272
NdeII GATC 5 cut(s) 104, 162, 564, 601, 684
NlaIV GGNNCC 1 cut(s) 255
NmuCI GTSAC 1 cut(s) 397
PaeR7I CTCGAG 1 cut(s) 170
PceI AGGCCT 1 cut(s) 650
PdmI GAANNNNTTC 1 cut(s) 535
PkrI GCNGC 3 cut(s) 322, 360, 363
Ple19I CGATCG 1 cut(s) 107
PleI GAGTC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 123
Psp6I CCWGG 1 cut(s) 386
PspGI CCWGG 1 cut(s) 386
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 2 cut(s) 100, 666
PstI CTGCAG 1 cut(s) 289
PstNI CAGNNNCTG 1 cut(s) 751
PsuI RGATCY 1 cut(s) 162
PvuI CGATCG 1 cut(s) 107
RsaI GTAC 5 cut(s) 56, 406, 487, 707, 834
RsaNI GTAC 5 cut(s) 55, 405, 486, 706, 833
SacII CCGCGG 1 cut(s) 354
SaqAI TTAA 4 cut(s) 111, 429, 662, 764
SatI GCNGC 3 cut(s) 321, 359, 362
Sau3AI GATC 5 cut(s) 104, 162, 564, 601, 684
Sau96I GGNCC 2 cut(s) 100, 666
ScaI AGTACT 1 cut(s) 56
SchI GAGTC 1 cut(s) 123
ScrFI CCNGG 1 cut(s) 388
SfaNI GCATC 4 cut(s) 253, 467, 499, 689
SfcI CTRYAG 1 cut(s) 285
Sfr274I CTCGAG 1 cut(s) 170
Sfr303I CCGCGG 1 cut(s) 354
SgrBI CCGCGG 1 cut(s) 354
SinI GGWCC 2 cut(s) 100, 666
SlaI CTCGAG 1 cut(s) 170
SmlI CTYRAG 4 cut(s) 110, 131, 170, 661
SmoI CTYRAG 4 cut(s) 110, 131, 170, 661
SpeI ACTAGT 1 cut(s) 196
Sse9I AATT 4 cut(s) 314, 491, 625, 776
SseBI AGGCCT 1 cut(s) 650
SsiI CCGC 3 cut(s) 351, 353, 361
SspI AATATT 2 cut(s) 536, 729
SspMI CTAG 6 cut(s) 197, 258, 464, 545, 554, 620
StuI AGGCCT 1 cut(s) 650
StyD4I CCNGG 1 cut(s) 386
StyI CCWWGG 1 cut(s) 257
TaaI ACNGT 3 cut(s) 120, 753, 829
TaqI TCGA 4 cut(s) 171, 201, 567, 683
TaqII GACCGA 1 cut(s) 117
TasI AATT 4 cut(s) 314, 491, 625, 776
TatI WGTACW 3 cut(s) 54, 404, 832
TauI GCSGC 1 cut(s) 364
Tru1I TTAA 4 cut(s) 111, 429, 662, 764
Tru9I TTAA 4 cut(s) 111, 429, 662, 764
TscAI CASTG 1 cut(s) 567
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 2 cut(s) 320, 358
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 1 cut(s) 539
TspGWI ACGGA 1 cut(s) 487
TspRI CASTG 1 cut(s) 567
Vha464I CTTAAG 2 cut(s) 110, 661
VpaK11BI GGWCC 2 cut(s) 100, 666
XapI RAATTY 1 cut(s) 625
XbaI TCTAGA 1 cut(s) 553
XhoI CTCGAG 1 cut(s) 170
XmaJI CCTAGG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 326
XmnI GAANNNNTTC 1 cut(s) 535
XspI CTAG 6 cut(s) 197, 258, 464, 545, 554, 620
ZrmI AGTACT 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.