Rw4G013550
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0018775
Physical Location & Seq
Forward (+)
31675331 .. 31676637
1307 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G013550.1

Sequence Viewer

Length: 1074 bp
ATGGGTGGGGAGCTTGTTGCAGGTGCTGTTTTAGGAACACTGTTCGCCGAGCTGTACAAGGGGTTCAAGGAAATAAGAGGCGAGACTAAGCGCTTTAGAGGCCAGCTAGAGAATCTCAATTGCACCCTTGACTCTCTGAAACCTCTGATCTTCCAGATAGGTGAGCTGAACTTGAAACTGGATCTCCCAACTTCGGAAATTGAAGATCTCATGAGAGAGATGGAGAAGGGTGCAGAGCTGGTAGGCAGACTGAGCCAGATTCGGAAGAGGGAGTACTTGTTAGTCAAGCGACGTTATAGCAACCAGCTTGTTAAACTGGATCAATCGCTTAAGATACTGTTGGATAGACTCAAGTTTCAGGAAGCAAGGGATGTGAAGGAGATCTATCTCGAGATGAGAAAACAAACGGCAAAACTAGTCCAAATGGAGGAGCTTCTCAAAATGCTTGGGAAGAAACTGAATGTGAGGCAGGAGCCTAGGGATGCGAAGGTGGCGTTGCTTTCTGCAGTAGAGAGCAGTAAACAGCTTGTTGAATTGGCAGCGTCTATTAAACTACTGCTTGGTAAACCCTTGTTGCAGCGGCAAGCAAGAAATGTGGAGGAGACCAGGATTTTGGTGACGAGTACAGAGATGGCGCTGGAGCGAGTTAAAGAGAATGGTGTCTTGAAAGGTTTGGCATCGCTAGTGCAACGGGCTGCTGTGGGTACAATATTGCAAGAGCTGTTTGATGCGCTATATAAGATGAAGGGAAGATATTCCAAGCTAGAGTTTCTACAACCACTGATCGAAGAAATGCAGGCAAAATATAACAATGTCTTGGATCACCCAAAAGAAGGACTAGAAAATTTCAAAATGCTTGTGGAAAAGGCCTTGCACCTCTTACGGTCCAAGCTTCGTGCAGGGATCAGCTACAAAAAGCACAAGTACGGCAACCAGCTACGTGCAATATTGGATAAATCTCTTCAGAAACTGTTGGACATATTAAAAGTGCAGGAAATGAGTAACACCAACGAGACGACCTTGGTTTCAGTCAGGAATATGGAGACAGTAGTACAAAGATTGTTGGACGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

357

Amino Acids

40.94

Weight (kDa)

9.6

Isoelectric Point (pI)

33.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 4 - 139 1.1e-26 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 227 - 350 6.6e-06 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018775)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15300
rosa_laevigata RLG00000008404 RLG00000008405 RLG00000017331
rosa_multiflora Rmu_co8367303.1_g000001
rosa_roxburghii Rroxscaffold_2G00138970
rosa_samantha Rh4AG162700 Rh4DG158400
rosa_wichuraiana Rw4G013550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 11
Acc36I ACCTGC 1 cut(s) 11
AciI CCGC 1 cut(s) 580
AclWI GGATC 4 cut(s) 189, 327, 828, 911
AcsI RAATTY 1 cut(s) 844
AcuI CTGAAG 1 cut(s) 947
AfaI GTAC 6 cut(s) 56, 275, 625, 706, 926, 1053
AfeI AGCGCT 1 cut(s) 92
AfiI CCNNNNNNNGG 3 cut(s) 193, 427, 833
AflII CTTAAG 1 cut(s) 329
AgsI TTSAA 6 cut(s) 67, 175, 203, 533, 667, 850
AhlI ACTAGT 1 cut(s) 415
AjnI CCWGG 1 cut(s) 605
Alw26I GTCTC 4 cut(s) 77, 596, 1007, 1037
AlwI GGATC 4 cut(s) 189, 327, 828, 911
AlwNI CAGNNNCTG 2 cut(s) 26, 970
Ama87I CYCGRG 1 cut(s) 389
Aor51HI AGCGCT 1 cut(s) 92
AoxI GGCC 2 cut(s) 100, 867
ApeKI GCWGC 3 cut(s) 539, 577, 695
ApoI RAATTY 1 cut(s) 844
ArsI GACNNNNNNTTYG 2 cut(s) 595, 627
Asp700I GAANNNNTTC 1 cut(s) 754
AspA2I CCTAGG 1 cut(s) 476
AspLEI GCGC 3 cut(s) 93, 637, 733
AspS9I GGNCC 1 cut(s) 885
AsuHPI GGTGA 3 cut(s) 173, 628, 815
AvaI CYCGRG 1 cut(s) 389
AvaII GGWCC 1 cut(s) 885
AvrII CCTAGG 1 cut(s) 476
BarI GAAGNNNNNNTAC 2 cut(s) 257, 289
BbvI GCAGC 3 cut(s) 551, 589, 682
BccI CCATC 2 cut(s) 214, 625
BceAI ACGGC 2 cut(s) 423, 943
BciT130I CCWGG 1 cut(s) 607
BcoDI GTCTC 4 cut(s) 77, 596, 1007, 1037
BcuI ACTAGT 1 cut(s) 415
BfaI CTAG 6 cut(s) 107, 416, 477, 683, 764, 839
BfmI CTRYAG 1 cut(s) 504
BfoI RGCGCY 2 cut(s) 94, 638
BfrI CTTAAG 1 cut(s) 329
BfuAI ACCTGC 1 cut(s) 11
BglII AGATCT 2 cut(s) 205, 381
BisI GCNGC 4 cut(s) 540, 578, 581, 696
BlnI CCTAGG 1 cut(s) 476
BlsI GCNGC 4 cut(s) 541, 579, 582, 697
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 1 cut(s) 607
Bme18I GGWCC 1 cut(s) 885
BmeT110I CYCGRG 1 cut(s) 389
BmgT120I GGNCC 1 cut(s) 885
BmiI GGNNCC 1 cut(s) 474
BmrFI CCNGG 1 cut(s) 607
BmsI GCATC 3 cut(s) 472, 686, 718
BpmI CTGGAG 1 cut(s) 659
BpuEI CTTGAG 1 cut(s) 335
BsaAI YACGTR 1 cut(s) 941
BsaI GGTCTC 1 cut(s) 596
BsaJI CCNNGG 2 cut(s) 476, 1020
BsaXI ACNNNNNCTCC 4 cut(s) 168, 198, 263, 293
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 427, 833
Bse1I ACTGG 2 cut(s) 183, 321
BseBI CCWGG 1 cut(s) 607
BseDI CCNNGG 2 cut(s) 476, 1020
BseGI GGATG 2 cut(s) 376, 487
BseLI CCNNNNNNNGG 3 cut(s) 193, 427, 833
BseMII CTCAG 1 cut(s) 242
BseNI ACTGG 2 cut(s) 183, 321
BseRI GAGGAG 2 cut(s) 443, 614
BseXI GCAGC 3 cut(s) 551, 589, 682
BsgI GTGCAG 3 cut(s) 252, 918, 1010
BshFI GGCC 2 cut(s) 102, 869
BsiHKCI CYCGRG 1 cut(s) 389
BslI CCNNNNNNNGG 3 cut(s) 193, 427, 833
BsmAI GTCTC 4 cut(s) 77, 596, 1007, 1037
BsmBI CGTCTC 1 cut(s) 1007
BsnI GGCC 2 cut(s) 102, 869
Bso31I GGTCTC 1 cut(s) 596
BsoBI CYCGRG 1 cut(s) 389
Bsp1407I TGTACA 1 cut(s) 54
Bsp143I GATC 8 cut(s) 147, 181, 205, 319, 381, 783, 820, 903
BspACI CCGC 1 cut(s) 580
BspANI GGCC 2 cut(s) 102, 869
BspCNI CTCAG 1 cut(s) 243
BspHI TCATGA 1 cut(s) 210
BspLI GGNNCC 1 cut(s) 474
BspMAI CTGCAG 1 cut(s) 508
BspMI ACCTGC 1 cut(s) 11
BspPI GGATC 4 cut(s) 189, 327, 828, 911
BspTI CTTAAG 1 cut(s) 329
BspTNI GGTCTC 1 cut(s) 596
BsrGI TGTACA 1 cut(s) 54
BsrI ACTGG 2 cut(s) 183, 321
BssECI CCNNGG 2 cut(s) 476, 1020
BssMI GATC 8 cut(s) 147, 181, 205, 319, 381, 783, 820, 903
BssT1I CCWWGG 2 cut(s) 476, 1020
Bst2UI CCWGG 1 cut(s) 607
Bst4CI ACNGT 5 cut(s) 42, 339, 885, 972, 1048
Bst6I CTCTTC 2 cut(s) 260, 966
BstAFI CTTAAG 1 cut(s) 329
BstAUI TGTACA 1 cut(s) 54
BstBAI YACGTR 1 cut(s) 941
BstC8I GCNNGC 3 cut(s) 104, 585, 798
BstDEI CTNAG 2 cut(s) 87, 251
BstF5I GGATG 2 cut(s) 376, 487
BstH2I RGCGCY 2 cut(s) 94, 638
BstHHI GCGC 3 cut(s) 93, 637, 733
BstKTI GATC 8 cut(s) 150, 184, 208, 322, 384, 786, 823, 906
BstMAI GTCTC 4 cut(s) 77, 596, 1007, 1037
BstMBI GATC 8 cut(s) 147, 181, 205, 319, 381, 783, 820, 903
BstMWI GCNNNNNNNGC 3 cut(s) 99, 252, 491
BstNI CCWGG 1 cut(s) 607
BstSCI CCNGG 1 cut(s) 605
BstSFI CTRYAG 1 cut(s) 504
BstV1I GCAGC 3 cut(s) 551, 589, 682
BstX2I RGATCY 3 cut(s) 181, 205, 381
BstXI CCANNNNNNTGG 1 cut(s) 613
BstYI RGATCY 3 cut(s) 181, 205, 381
BsuRI GGCC 2 cut(s) 102, 869
BtgZI GCGATG 1 cut(s) 663
BtsCI GGATG 2 cut(s) 376, 487
BtsIMutI CAGTG 2 cut(s) 38, 779
BveI ACCTGC 1 cut(s) 11
Cac8I GCNNGC 3 cut(s) 104, 585, 798
CaiI CAGNNNCTG 2 cut(s) 26, 970
CciI TCATGA 1 cut(s) 210
CfoI GCGC 3 cut(s) 93, 637, 733
Cfr13I GGNCC 1 cut(s) 885
CseI GACGC 1 cut(s) 531
Csp6I GTAC 6 cut(s) 55, 274, 624, 705, 925, 1052
CspCI CAANNNNNGTGG 2 cut(s) 576, 611
CviAII CATG 1 cut(s) 211
CviQI GTAC 6 cut(s) 55, 274, 624, 705, 925, 1052
DdeI CTNAG 2 cut(s) 87, 251
DpnI GATC 8 cut(s) 149, 183, 207, 321, 383, 785, 822, 905
DpnII GATC 8 cut(s) 147, 181, 205, 319, 381, 783, 820, 903
Eam1104I CTCTTC 2 cut(s) 260, 966
EarI CTCTTC 2 cut(s) 260, 966
Eco130I CCWWGG 2 cut(s) 476, 1020
Eco147I AGGCCT 1 cut(s) 869
Eco31I GGTCTC 1 cut(s) 596
Eco47I GGWCC 1 cut(s) 885
Eco47III AGCGCT 1 cut(s) 92
Eco57I CTGAAG 1 cut(s) 947
Eco88I CYCGRG 1 cut(s) 389
EcoRII CCWGG 1 cut(s) 605
EcoT14I CCWWGG 2 cut(s) 476, 1020
ErhI CCWWGG 2 cut(s) 476, 1020
Esp3I CGTCTC 1 cut(s) 1007
FaeI CATG 1 cut(s) 214
FaiI YATR 8 cut(s) 212, 297, 736, 738, 807, 980, 1040, 1072
FatI CATG 1 cut(s) 210
Fnu4HI GCNGC 4 cut(s) 540, 578, 581, 696
FokI GGATG 2 cut(s) 383, 494
Fsp4HI GCNGC 4 cut(s) 540, 578, 581, 696
FspBI CTAG 6 cut(s) 107, 416, 477, 683, 764, 839
GlaI GCGC 3 cut(s) 92, 636, 732
GluI GCNGC 4 cut(s) 540, 578, 581, 696
GsuI CTGGAG 1 cut(s) 659
HaeII RGCGCY 2 cut(s) 94, 638
HaeIII GGCC 2 cut(s) 102, 869
HgaI GACGC 1 cut(s) 531
HhaI GCGC 3 cut(s) 93, 637, 733
Hin1II CATG 1 cut(s) 214
Hin6I GCGC 3 cut(s) 91, 635, 731
HinP1I GCGC 3 cut(s) 91, 635, 731
HindIII AAGCTT 1 cut(s) 890
HinfI GANTC 4 cut(s) 112, 131, 259, 348
HphI GGTGA 3 cut(s) 173, 628, 815
Hpy166II GTNNAC 2 cut(s) 521, 566
Hpy188I TCNGA 5 cut(s) 138, 147, 196, 264, 966
Hpy188III TCNNGA 7 cut(s) 154, 211, 359, 389, 391, 664, 1033
Hpy8I GTNNAC 2 cut(s) 521, 566
Hpy99I CGWCG 1 cut(s) 294
HpyAV CCTTC 5 cut(s) 220, 370, 481, 739, 827
HpyCH4III ACNGT 5 cut(s) 42, 339, 885, 972, 1048
HpyCH4IV ACGT 3 cut(s) 292, 940, 1068
HpyF10VI GCNNNNNNNGC 3 cut(s) 99, 252, 491
HpyF3I CTNAG 2 cut(s) 87, 251
HpySE526I ACGT 3 cut(s) 292, 940, 1068
Hsp92II CATG 1 cut(s) 214
HspAI GCGC 3 cut(s) 91, 635, 731
Kzo9I GATC 8 cut(s) 147, 181, 205, 319, 381, 783, 820, 903
LmnI GCTCC 4 cut(s) 10, 430, 472, 640
Lsp1109I GCAGC 3 cut(s) 551, 589, 682
LweI GCATC 3 cut(s) 472, 686, 718
MaeI CTAG 6 cut(s) 107, 416, 477, 683, 764, 839
MaeII ACGT 3 cut(s) 292, 940, 1068
MaeIII GTNAC 2 cut(s) 616, 1001
MalI GATC 8 cut(s) 149, 183, 207, 321, 383, 785, 822, 905
MboI GATC 8 cut(s) 147, 181, 205, 319, 381, 783, 820, 903
MboII GAAGA 7 cut(s) 142, 215, 277, 463, 762, 800, 953
MfeI CAATTG 1 cut(s) 118
MflI RGATCY 3 cut(s) 181, 205, 381
MluCI AATT 4 cut(s) 118, 198, 533, 844
MlyI GAGTC 2 cut(s) 125, 342
MmeI TCCRAC 3 cut(s) 321, 954, 1044
MnlI CCTC 8 cut(s) 71, 92, 153, 261, 421, 459, 592, 887
MroXI GAANNNNTTC 1 cut(s) 754
MseI TTAA 5 cut(s) 312, 330, 549, 648, 983
MspA1I CMGCKG 1 cut(s) 580
MspCI CTTAAG 1 cut(s) 329
MspR9I CCNGG 1 cut(s) 607
MunI CAATTG 1 cut(s) 118
MvaI CCWGG 1 cut(s) 607
MwoI GCNNNNNNNGC 3 cut(s) 99, 252, 491
NdeII GATC 8 cut(s) 147, 181, 205, 319, 381, 783, 820, 903
NlaIII CATG 1 cut(s) 214
NlaIV GGNNCC 1 cut(s) 474
NmeAIII GCCGAG 1 cut(s) 73
NmuCI GTSAC 1 cut(s) 616
PaeR7I CTCGAG 1 cut(s) 389
PagI TCATGA 1 cut(s) 210
PaqCI CACCTGC 1 cut(s) 11
PceI AGGCCT 1 cut(s) 869
PdmI GAANNNNTTC 1 cut(s) 754
PfeI GAWTC 2 cut(s) 112, 259
PkrI GCNGC 4 cut(s) 541, 579, 582, 697
PleI GAGTC 2 cut(s) 125, 342
PpsI GAGTC 2 cut(s) 125, 342
Ppu21I YACGTR 1 cut(s) 941
Psp6I CCWGG 1 cut(s) 605
PspGI CCWGG 1 cut(s) 605
PspN4I GGNNCC 1 cut(s) 474
PspPI GGNCC 1 cut(s) 885
PsrI GAACNNNNNNTAC 2 cut(s) 47, 79
PstI CTGCAG 1 cut(s) 508
PstNI CAGNNNCTG 2 cut(s) 26, 970
PsuI RGATCY 3 cut(s) 181, 205, 381
RsaI GTAC 6 cut(s) 56, 275, 625, 706, 926, 1053
RsaNI GTAC 6 cut(s) 55, 274, 624, 705, 925, 1052
SaqAI TTAA 5 cut(s) 312, 330, 549, 648, 983
SatI GCNGC 4 cut(s) 540, 578, 581, 696
Sau3AI GATC 8 cut(s) 147, 181, 205, 319, 381, 783, 820, 903
Sau96I GGNCC 1 cut(s) 885
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 2 cut(s) 125, 342
ScrFI CCNGG 1 cut(s) 607
SfaNI GCATC 3 cut(s) 472, 686, 718
SfcI CTRYAG 1 cut(s) 504
Sfr274I CTCGAG 1 cut(s) 389
SinI GGWCC 1 cut(s) 885
SlaI CTCGAG 1 cut(s) 389
SmlI CTYRAG 3 cut(s) 329, 350, 389
SmoI CTYRAG 3 cut(s) 329, 350, 389
SpeI ACTAGT 1 cut(s) 415
Sse9I AATT 4 cut(s) 118, 198, 533, 844
SseBI AGGCCT 1 cut(s) 869
SsiI CCGC 1 cut(s) 580
SspI AATATT 2 cut(s) 711, 948
SspMI CTAG 6 cut(s) 107, 416, 477, 683, 764, 839
StuI AGGCCT 1 cut(s) 869
StyD4I CCNGG 1 cut(s) 605
StyI CCWWGG 2 cut(s) 476, 1020
TaaI ACNGT 5 cut(s) 42, 339, 885, 972, 1048
TaiI ACGT 3 cut(s) 295, 943, 1071
TaqI TCGA 2 cut(s) 390, 786
TasI AATT 4 cut(s) 118, 198, 533, 844
TatI WGTACW 4 cut(s) 54, 273, 623, 1051
TauI GCSGC 1 cut(s) 583
TfiI GAWTC 2 cut(s) 112, 259
Tru1I TTAA 5 cut(s) 312, 330, 549, 648, 983
Tru9I TTAA 5 cut(s) 312, 330, 549, 648, 983
TscAI CASTG 2 cut(s) 45, 786
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 3 cut(s) 539, 577, 695
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 1 cut(s) 758
TspRI CASTG 2 cut(s) 45, 786
Vha464I CTTAAG 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 885
XapI RAATTY 1 cut(s) 844
XhoI CTCGAG 1 cut(s) 389
XmaJI CCTAGG 1 cut(s) 476
XmnI GAANNNNTTC 1 cut(s) 754
XspI CTAG 6 cut(s) 107, 416, 477, 683, 764, 839
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.