RLG00000008405

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0018775
Physical Location & Seq
Reverse (-)
30108484 .. 30108882
399 bp
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UTR
Exon/CDS
Intron
RLM00000008405

Sequence Viewer

Length: 399 bp
ATGGCAATGGTGGGGGATCTTGTTGCAGGAGCTGTTTTAGGAACACTGTTCGCTGAGCTGTACAAGGGGTTCAAGGAAATAAGAGGCGAGACTAAGCGCTTTAGAGGCCACCTAGAGAATCTCAATTGCACCCTTGACTCTCTGAAACCTCTGATCTTCCAGATAGGTGAGCTGAACTTGAAACTGGATCTCCCAACTTCGGAAATTGAAGATCTCATGAGAGAGATGGGGAAGGGCACAGAGCTGGTAGGCAGACTGAGCCAGATTCGGAAGAGGGAGTACTTGTTAGTCAAGCGACGTTATAACAACCAGCTTGTTAAACTGGATCAATCGCTTAAGATACTGTTGGATAGACTCAAGTTTCAGGAAGCAAGGGATGTGAAGGAGATCTATCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

133

Amino Acids

15.28

Weight (kDa)

9.24

Isoelectric Point (pI)

30.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 130 6.5e-24 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018775)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15300
rosa_laevigata RLG00000008404 RLG00000008405 RLG00000017331
rosa_multiflora Rmu_co8367303.1_g000001
rosa_roxburghii Rroxscaffold_2G00138970
rosa_samantha Rh4AG162700 Rh4DG158400
rosa_wichuraiana Rw4G013550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AclWI GGATC 3 cut(s) 24, 195, 333
AfaI GTAC 2 cut(s) 62, 281
AfeI AGCGCT 1 cut(s) 98
AfiI CCNNNNNNNGG 1 cut(s) 199
AflII CTTAAG 1 cut(s) 335
AgsI TTSAA 3 cut(s) 73, 181, 209
AluBI AGCT 5 cut(s) 32, 58, 172, 244, 313
AluI AGCT 5 cut(s) 32, 58, 172, 244, 313
Alw26I GTCTC 1 cut(s) 83
AlwI GGATC 3 cut(s) 24, 195, 333
AlwNI CAGNNNCTG 1 cut(s) 32
Aor51HI AGCGCT 1 cut(s) 98
AoxI GGCC 1 cut(s) 106
AspLEI GCGC 1 cut(s) 99
AsuHPI GGTGA 1 cut(s) 179
BaeGI GKGCMC 1 cut(s) 239
BarI GAAGNNNNNNTAC 2 cut(s) 263, 295
BccI CCATC 1 cut(s) 220
BcoDI GTCTC 1 cut(s) 83
BfaI CTAG 2 cut(s) 113, 397
BfoI RGCGCY 1 cut(s) 100
BfrI CTTAAG 1 cut(s) 335
BglII AGATCT 2 cut(s) 211, 387
BlpI GCTNAGC 1 cut(s) 54
BmcAI AGTACT 1 cut(s) 281
Bpu1102I GCTNAGC 1 cut(s) 54
BpuEI CTTGAG 1 cut(s) 341
BsaXI ACNNNNNCTCC 4 cut(s) 174, 204, 269, 299
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse1I ACTGG 2 cut(s) 189, 327
Bse3DI GCAATG 1 cut(s) 12
BseGI GGATG 1 cut(s) 382
BseLI CCNNNNNNNGG 1 cut(s) 199
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 2 cut(s) 45, 248
BseNI ACTGG 2 cut(s) 189, 327
BseSI GKGCMC 1 cut(s) 239
BshFI GGCC 1 cut(s) 108
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 1 cut(s) 83
BsnI GGCC 1 cut(s) 108
Bsp1286I GDGCHC 1 cut(s) 239
Bsp1407I TGTACA 1 cut(s) 60
Bsp143I GATC 6 cut(s) 16, 153, 187, 211, 325, 387
Bsp1720I GCTNAGC 1 cut(s) 54
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 2 cut(s) 46, 249
BspHI TCATGA 1 cut(s) 216
BspPI GGATC 3 cut(s) 24, 195, 333
BspTI CTTAAG 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 12
BsrGI TGTACA 1 cut(s) 60
BsrI ACTGG 2 cut(s) 189, 327
BssMI GATC 6 cut(s) 16, 153, 187, 211, 325, 387
Bst4CI ACNGT 2 cut(s) 48, 345
Bst6I CTCTTC 1 cut(s) 266
BstAFI CTTAAG 1 cut(s) 335
BstAUI TGTACA 1 cut(s) 60
BstDEI CTNAG 3 cut(s) 54, 93, 257
BstF5I GGATG 1 cut(s) 382
BstH2I RGCGCY 1 cut(s) 100
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 6 cut(s) 19, 156, 190, 214, 328, 390
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 6 cut(s) 16, 153, 187, 211, 325, 387
BstMWI GCNNNNNNNGC 2 cut(s) 105, 258
BstSLI GKGCMC 1 cut(s) 239
BstX2I RGATCY 4 cut(s) 16, 187, 211, 387
BstYI RGATCY 4 cut(s) 16, 187, 211, 387
BsuRI GGCC 1 cut(s) 108
BtsCI GGATG 1 cut(s) 382
BtsIMutI CAGTG 1 cut(s) 44
CaiI CAGNNNCTG 1 cut(s) 32
CciI TCATGA 1 cut(s) 216
CfoI GCGC 1 cut(s) 99
Csp6I GTAC 2 cut(s) 61, 280
CviAII CATG 1 cut(s) 217
CviJI RGCY 7 cut(s) 32, 58, 108, 172, 244, 261, 313
CviKI_1 RGCY 7 cut(s) 32, 58, 108, 172, 244, 261, 313
CviQI GTAC 2 cut(s) 61, 280
DdeI CTNAG 3 cut(s) 54, 93, 257
DpnI GATC 6 cut(s) 18, 155, 189, 213, 327, 389
DpnII GATC 6 cut(s) 16, 153, 187, 211, 325, 387
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
Eco47III AGCGCT 1 cut(s) 98
FaeI CATG 1 cut(s) 220
FaiI YATR 2 cut(s) 218, 303
FatI CATG 1 cut(s) 216
FokI GGATG 1 cut(s) 389
FspBI CTAG 2 cut(s) 113, 397
GlaI GCGC 1 cut(s) 98
HaeII RGCGCY 1 cut(s) 100
HaeIII GGCC 1 cut(s) 108
HhaI GCGC 1 cut(s) 99
Hin1II CATG 1 cut(s) 220
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HinfI GANTC 4 cut(s) 118, 137, 265, 354
HphI GGTGA 1 cut(s) 179
Hpy188I TCNGA 4 cut(s) 144, 153, 202, 270
Hpy188III TCNNGA 3 cut(s) 160, 217, 365
Hpy99I CGWCG 1 cut(s) 300
HpyAV CCTTC 2 cut(s) 226, 376
HpyCH4III ACNGT 2 cut(s) 48, 345
HpyCH4IV ACGT 1 cut(s) 298
HpyCH4V TGCA 2 cut(s) 26, 129
HpyF10VI GCNNNNNNNGC 2 cut(s) 105, 258
HpyF3I CTNAG 3 cut(s) 54, 93, 257
HpySE526I ACGT 1 cut(s) 298
Hsp92II CATG 1 cut(s) 220
HspAI GCGC 1 cut(s) 97
Kzo9I GATC 6 cut(s) 16, 153, 187, 211, 325, 387
LmnI GCTCC 1 cut(s) 29
LpnPI CCDG 8 cut(s) 12, 170, 173, 230, 275, 308, 323, 350
MaeI CTAG 2 cut(s) 113, 397
MaeII ACGT 1 cut(s) 298
MalI GATC 6 cut(s) 18, 155, 189, 213, 327, 389
MboI GATC 6 cut(s) 16, 153, 187, 211, 325, 387
MboII GAAGA 3 cut(s) 148, 221, 283
MfeI CAATTG 1 cut(s) 124
MflI RGATCY 4 cut(s) 16, 187, 211, 387
MhlI GDGCHC 1 cut(s) 239
MluCI AATT 2 cut(s) 124, 204
MlyI GAGTC 2 cut(s) 131, 348
MmeI TCCRAC 1 cut(s) 327
MnlI CCTC 4 cut(s) 77, 98, 159, 267
MseI TTAA 2 cut(s) 318, 336
MspCI CTTAAG 1 cut(s) 335
MunI CAATTG 1 cut(s) 124
MwoI GCNNNNNNNGC 2 cut(s) 105, 258
NdeII GATC 6 cut(s) 16, 153, 187, 211, 325, 387
NlaIII CATG 1 cut(s) 220
PagI TCATGA 1 cut(s) 216
PfeI GAWTC 2 cut(s) 118, 265
PleI GAGTC 2 cut(s) 131, 348
PpsI GAGTC 2 cut(s) 131, 348
PsiI TTATAA 1 cut(s) 303
PsrI GAACNNNNNNTAC 2 cut(s) 53, 85
PstNI CAGNNNCTG 1 cut(s) 32
PsuI RGATCY 4 cut(s) 16, 187, 211, 387
RsaI GTAC 2 cut(s) 62, 281
RsaNI GTAC 2 cut(s) 61, 280
SaqAI TTAA 2 cut(s) 318, 336
Sau3AI GATC 6 cut(s) 16, 153, 187, 211, 325, 387
ScaI AGTACT 1 cut(s) 281
SchI GAGTC 2 cut(s) 131, 348
SduI GDGCHC 1 cut(s) 239
SetI ASST 9 cut(s) 34, 60, 114, 151, 169, 174, 246, 301, 315
SmlI CTYRAG 2 cut(s) 335, 356
SmoI CTYRAG 2 cut(s) 335, 356
Sse9I AATT 2 cut(s) 124, 204
SspMI CTAG 2 cut(s) 113, 397
TaaI ACNGT 2 cut(s) 48, 345
TaiI ACGT 1 cut(s) 301
TasI AATT 2 cut(s) 124, 204
TatI WGTACW 2 cut(s) 60, 279
TfiI GAWTC 2 cut(s) 118, 265
Tru1I TTAA 2 cut(s) 318, 336
Tru9I TTAA 2 cut(s) 318, 336
TscAI CASTG 1 cut(s) 51
TspRI CASTG 1 cut(s) 51
Vha464I CTTAAG 1 cut(s) 335
XspI CTAG 2 cut(s) 113, 397
ZrmI AGTACT 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.