FvH4_1g18900

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004282
Physical Location & Seq
Reverse (-)
11189474 .. 11195043
5570 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g18900.t5

Sequence Viewer

Length: 1113 bp
ATGTCTCCACCACTGCTGGGTGGTGAGGGGGAAGGGAAGAGCAATGGATCTATGGTAGCTTGTTCGCCCTCTATGGAAATGATCCCCCAGAACAGCTCGGGATTGAAAGAGCGTAACTACCTTGGGTTGTCAGATTGTTCTTCAGTTGACAGCTCCACGGTCTCTAGCTTGTCAGAGGAGAATAAGAGCAAGTTCAATTTTAAGGCTACAGAGTTGAGGCTTGGTCTTCCTGGATCCCAATCACCAGAACGGCAATCAGAGCTTTGCTTGCTTGATGAAAAGCCACTGTTTCCTCTGCTTCCTTCAAAAGATGGAATCTGTTCCTCATCCCAGAAAAATGTTGTTTCAGGCAACAAAAGGGGCTTTTCTGATACCATGGATGGATTCTCAGAGGTGAAAAGTTCTGTTTATAATGAAGGAAAGTGGATGTTTCCTGCTGCTGCTGGTTCTGATTCCGAATCACCAGAATCTGTCGGACAAGGGAAGTTTCCTGTGAGTTCAATCAATGTGATGCTATCATCCAGGACTTCTGGGACTCAGCCAACCAATCTTGTGACCAATTCTTCAAAAGATCAGTCCAAAACTGCAAATGGAGCTAGCCAGAACCATGCCGTTGCTTCTAACAACAGCAGTGCTCCAGCTGCTAAGGCACAAGTTGTAGGGTGGCCTCCAATAAGATCTTTTAGGAAGAATTTAACCACTGCTTCAAAGAACAGTGATGAAGTTGATGGAAAACCTGGTCGAGGTGCTCTTTTTGTCAAGGTCAGCATGGACGGTGCTCCCTACCTGAGGAAGGTAGATCTGAGAACCTACGGAACATATCAAGAGTTGTCTTCTGCTCTGGAAAAGATGTTCAGCTGTTTTACAATTGGGCAATATGGATCCCATGGAGCTCCAGGGAGGGAAAGATTGAGTGAGAGTAAGCTGAGAGATCTTCTTCATGGATCAGAATATGTGCTTACATATGAGGACAAGGATGGTGATTGGATGCTTGTCGGAGATGTTCCGTGGGAGATGTTTATTGATTCATGCAAGAGGTTGAAGATAATGAAGGGCTCTGATGCCATTGGCTTAGCTCCTAGGTCCATGGAGAAATCCAAGATCCGGAACTAG

Protein Analysis

371

Amino Acids

39.84

Weight (kDa)

8.09

Isoelectric Point (pI)

48.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 69 - 361 1.5e-90 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 411
AccIII TCCGGA 1 cut(s) 1104
AclWI GGATC 8 cut(s) 55, 76, 228, 241, 876, 889, 952, 1096
AcsI RAATTY 1 cut(s) 691
AcuI CTGAAG 1 cut(s) 126
AfiI CCNNNNNNNGG 5 cut(s) 17, 743, 789, 793, 1104
AgsI TTSAA 7 cut(s) 106, 196, 306, 501, 567, 708, 1042
AjnI CCWGG 4 cut(s) 229, 521, 736, 895
AloI GAACNNNNNNTCC 2 cut(s) 836, 868
Alw21I GWGCWC 4 cut(s) 637, 751, 781, 895
Alw26I GTCTC 2 cut(s) 9, 166
AlwI GGATC 8 cut(s) 55, 76, 228, 241, 876, 889, 952, 1096
AlwNI CAGNNNCTG 1 cut(s) 470
Ama87I CYCGRG 1 cut(s) 97
Aor13HI TCCGGA 1 cut(s) 1104
AoxI GGCC 1 cut(s) 665
ApeKI GCWGC 3 cut(s) 437, 440, 641
ApoI RAATTY 1 cut(s) 691
ArsI GACNNNNNNTTYG 2 cut(s) 560, 592
Asp700I GAANNNNTTC 1 cut(s) 319
AspA2I CCTAGG 1 cut(s) 1079
AspS9I GGNCC 1 cut(s) 1083
AsuHPI GGTGA 5 cut(s) 35, 234, 406, 453, 992
AsuNHI GCTAGC 1 cut(s) 596
AvaI CYCGRG 1 cut(s) 97
AvaII GGWCC 1 cut(s) 1083
AvrII CCTAGG 1 cut(s) 1079
AxyI CCTNAGG 1 cut(s) 788
BamHI GGATCC 2 cut(s) 233, 881
BanII GRGCYC 2 cut(s) 895, 1058
BbsI GAAGAC 2 cut(s) 218, 825
Bbv12I GWGCWC 4 cut(s) 637, 751, 781, 895
BbvI GCAGC 3 cut(s) 424, 427, 628
BccI CCATC 4 cut(s) 305, 374, 722, 971
BceAI ACGGC 2 cut(s) 266, 596
BciT130I CCWGG 4 cut(s) 231, 523, 738, 897
BcoDI GTCTC 2 cut(s) 9, 166
BfaI CTAG 4 cut(s) 165, 597, 1080, 1111
BfmI CTRYAG 1 cut(s) 207
BglII AGATCT 3 cut(s) 677, 799, 931
BisI GCNGC 3 cut(s) 438, 441, 642
BlnI CCTAGG 1 cut(s) 1079
BlpI GCTNAGC 1 cut(s) 1072
BlsI GCNGC 3 cut(s) 439, 442, 643
Bme1390I CCNGG 4 cut(s) 231, 523, 738, 897
Bme18I GGWCC 1 cut(s) 1083
BmeT110I CYCGRG 1 cut(s) 97
BmgT120I GGNCC 1 cut(s) 1083
BmiI GGNNCC 2 cut(s) 235, 883
BmrFI CCNGG 4 cut(s) 231, 523, 738, 897
BmsI GCATC 3 cut(s) 501, 978, 1051
BmtI GCTAGC 1 cut(s) 600
BpiI GAAGAC 2 cut(s) 218, 825
BpmI CTGGAG 2 cut(s) 621, 879
Bpu10I CCTNAGC 1 cut(s) 645
Bpu1102I GCTNAGC 1 cut(s) 1072
BsaBI GATNNNNATC 1 cut(s) 238
BsaI GGTCTC 1 cut(s) 166
BsaJI CCNNGG 8 cut(s) 121, 156, 375, 886, 896, 1007, 1079, 1086
BsaWI WCCGGW 1 cut(s) 1104
Bsc4I CCNNNNNNNGG 5 cut(s) 17, 743, 789, 793, 1104
Bse21I CCTNAGG 1 cut(s) 788
Bse3DI GCAATG 1 cut(s) 49
Bse8I GATNNNNATC 1 cut(s) 238
BseAI TCCGGA 1 cut(s) 1104
BseBI CCWGG 4 cut(s) 231, 523, 738, 897
BseDI CCNNGG 8 cut(s) 121, 156, 375, 886, 896, 1007, 1079, 1086
BseGI GGATG 6 cut(s) 326, 385, 432, 518, 982, 993
BseJI GATNNNNATC 1 cut(s) 238
BseLI CCNNNNNNNGG 5 cut(s) 17, 743, 789, 793, 1104
BseMI GCAATG 1 cut(s) 49
BseMII CTCAG 5 cut(s) 402, 551, 779, 794, 917
BseRI GAGGAG 1 cut(s) 191
BseXI GCAGC 3 cut(s) 424, 427, 628
BseYI CCCAGC 1 cut(s) 16
BshFI GGCC 1 cut(s) 667
BsiHKAI GWGCWC 4 cut(s) 637, 751, 781, 895
BsiHKCI CYCGRG 1 cut(s) 97
BsiSI CCGG 1 cut(s) 1105
BslFI GGGAC 1 cut(s) 547
BslI CCNNNNNNNGG 5 cut(s) 17, 743, 789, 793, 1104
BsmAI GTCTC 2 cut(s) 9, 166
BsmFI GGGAC 1 cut(s) 547
BsnI GGCC 1 cut(s) 667
Bso31I GGTCTC 1 cut(s) 166
BsoBI CYCGRG 1 cut(s) 97
Bsp1286I GDGCHC 5 cut(s) 637, 751, 781, 895, 1058
Bsp13I TCCGGA 1 cut(s) 1104
Bsp1720I GCTNAGC 1 cut(s) 1072
Bsp19I CCATGG 3 cut(s) 375, 886, 1086
BspANI GGCC 1 cut(s) 667
BspCNI CTCAG 5 cut(s) 401, 550, 780, 795, 918
BspEI TCCGGA 1 cut(s) 1104
BspLI GGNNCC 2 cut(s) 235, 883
BspOI GCTAGC 1 cut(s) 600
BspPI GGATC 8 cut(s) 55, 76, 228, 241, 876, 889, 952, 1096
BspQI GCTCTTC 1 cut(s) 32
BspTNI GGTCTC 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 49
BssECI CCNNGG 8 cut(s) 121, 156, 375, 886, 896, 1007, 1079, 1086
BssT1I CCWWGG 5 cut(s) 121, 375, 886, 1079, 1086
Bst2UI CCWGG 4 cut(s) 231, 523, 738, 897
Bst4CI ACNGT 4 cut(s) 160, 288, 716, 776
Bst6I CTCTTC 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 269, 598
BstDEI CTNAG 7 cut(s) 388, 537, 645, 788, 803, 926, 1072
BstDSI CCRYGG 5 cut(s) 156, 375, 886, 1007, 1086
BstENI CCTNNNNNAGG 3 cut(s) 741, 787, 791
BstF5I GGATG 6 cut(s) 326, 385, 432, 518, 982, 993
BstMAI GTCTC 2 cut(s) 9, 166
BstMWI GCNNNNNNNGC 5 cut(s) 259, 268, 593, 641, 647
BstNI CCWGG 4 cut(s) 231, 523, 738, 897
BstSCI CCNGG 4 cut(s) 229, 521, 736, 895
BstSFI CTRYAG 1 cut(s) 207
BstV1I GCAGC 3 cut(s) 424, 427, 628
BstV2I GAAGAC 2 cut(s) 218, 825
BstX2I RGATCY 7 cut(s) 47, 233, 677, 799, 881, 931, 1101
BstYI RGATCY 7 cut(s) 47, 233, 677, 799, 881, 931, 1101
Bsu36I CCTNAGG 1 cut(s) 788
BsuRI GGCC 1 cut(s) 667
BtgI CCRYGG 5 cut(s) 156, 375, 886, 1007, 1086
BtsCI GGATG 6 cut(s) 326, 385, 432, 518, 982, 993
BtsI GCAGTG 3 cut(s) 11, 637, 699
BtsIMutI CAGTG 5 cut(s) 11, 284, 637, 699, 721
Cac8I GCNNGC 2 cut(s) 269, 598
CaiI CAGNNNCTG 1 cut(s) 470
Cfr13I GGNCC 1 cut(s) 1083
CsiI ACCWGGT 1 cut(s) 736
CviAII CATG 7 cut(s) 376, 608, 769, 887, 941, 1029, 1087
DdeI CTNAG 7 cut(s) 388, 537, 645, 788, 803, 926, 1072
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 893
Eco130I CCWWGG 5 cut(s) 121, 375, 886, 1079, 1086
Eco24I GRGCYC 2 cut(s) 895, 1058
Eco31I GGTCTC 1 cut(s) 166
Eco47I GGWCC 1 cut(s) 1083
Eco53kI GAGCTC 1 cut(s) 893
Eco57I CTGAAG 1 cut(s) 126
Eco81I CCTNAGG 1 cut(s) 788
Eco88I CYCGRG 1 cut(s) 97
EcoICRI GAGCTC 1 cut(s) 893
EcoNI CCTNNNNNAGG 3 cut(s) 741, 787, 791
EcoRII CCWGG 4 cut(s) 229, 521, 736, 895
EcoT14I CCWWGG 5 cut(s) 121, 375, 886, 1079, 1086
EcoT38I GRGCYC 2 cut(s) 895, 1058
ErhI CCWWGG 5 cut(s) 121, 375, 886, 1079, 1086
FaeI CATG 7 cut(s) 379, 611, 772, 890, 944, 1032, 1090
FaqI GGGAC 1 cut(s) 547
FatI CATG 7 cut(s) 375, 607, 768, 886, 940, 1028, 1086
FauNDI CATATG 1 cut(s) 964
Fnu4HI GCNGC 3 cut(s) 438, 441, 642
FokI GGATG 6 cut(s) 313, 392, 439, 505, 989, 1000
FriOI GRGCYC 2 cut(s) 895, 1058
Fsp4HI GCNGC 3 cut(s) 438, 441, 642
FspBI CTAG 4 cut(s) 165, 597, 1080, 1111
GluI GCNGC 3 cut(s) 438, 441, 642
GsaI CCCAGC 1 cut(s) 20
GsuI CTGGAG 2 cut(s) 621, 879
HaeIII GGCC 1 cut(s) 667
HapII CCGG 1 cut(s) 1105
Hin1II CATG 7 cut(s) 379, 611, 772, 890, 944, 1032, 1090
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 7 cut(s) 315, 384, 452, 458, 467, 535, 1025
HpaII CCGG 1 cut(s) 1105
HphI GGTGA 5 cut(s) 35, 234, 406, 453, 992
Hpy166II GTNNAC 1 cut(s) 148
Hpy188III TCNNGA 4 cut(s) 99, 824, 842, 1105
Hpy8I GTNNAC 1 cut(s) 148
HpyAV CCTTC 5 cut(s) 26, 312, 410, 787, 1045
HpyCH4III ACNGT 4 cut(s) 160, 288, 716, 776
HpyCH4V TGCA 2 cut(s) 587, 1032
HpyF10VI GCNNNNNNNGC 5 cut(s) 259, 268, 593, 641, 647
HpyF3I CTNAG 7 cut(s) 388, 537, 645, 788, 803, 926, 1072
Hsp92II CATG 7 cut(s) 379, 611, 772, 890, 944, 1032, 1090
Kpn2I TCCGGA 1 cut(s) 1104
LguI GCTCTTC 1 cut(s) 32
LmnI GCTCC 7 cut(s) 158, 593, 640, 784, 890, 898, 1081
Lsp1109I GCAGC 3 cut(s) 424, 427, 628
LweI GCATC 3 cut(s) 501, 978, 1051
MabI ACCWGGT 1 cut(s) 736
MaeI CTAG 4 cut(s) 165, 597, 1080, 1111
MaeIII GTNAC 2 cut(s) 113, 553
MboII GAAGA 9 cut(s) 49, 132, 218, 555, 700, 825, 926, 929, 1054
MfeI CAATTG 1 cut(s) 867
MflI RGATCY 7 cut(s) 47, 233, 677, 799, 881, 931, 1101
MhlI GDGCHC 5 cut(s) 637, 751, 781, 895, 1058
MluCI AATT 4 cut(s) 196, 559, 691, 867
MlyI GAGTC 1 cut(s) 529
MmeI TCCRAC 2 cut(s) 454, 976
MroI TCCGGA 1 cut(s) 1104
MroXI GAANNNNTTC 1 cut(s) 319
MseI TTAA 2 cut(s) 201, 695
MspA1I CMGCKG 2 cut(s) 641, 858
MspI CCGG 1 cut(s) 1105
MspR9I CCNGG 4 cut(s) 231, 523, 738, 897
MunI CAATTG 1 cut(s) 867
MvaI CCWGG 4 cut(s) 231, 523, 738, 897
MwoI GCNNNNNNNGC 5 cut(s) 259, 268, 593, 641, 647
NcoI CCATGG 3 cut(s) 375, 886, 1086
NdeI CATATG 1 cut(s) 964
NheI GCTAGC 1 cut(s) 596
NlaIII CATG 7 cut(s) 379, 611, 772, 890, 944, 1032, 1090
NlaIV GGNNCC 2 cut(s) 235, 883
NmuCI GTSAC 1 cut(s) 553
PciSI GCTCTTC 1 cut(s) 32
PdmI GAANNNNTTC 1 cut(s) 319
PfeI GAWTC 6 cut(s) 315, 384, 452, 458, 467, 1025
PfoI TCCNGGA 2 cut(s) 229, 521
PkrI GCNGC 3 cut(s) 439, 442, 643
PleI GAGTC 1 cut(s) 529
PpsI GAGTC 1 cut(s) 529
PsiI TTATAA 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 895
Psp6I CCWGG 4 cut(s) 229, 521, 736, 895
PspFI CCCAGC 1 cut(s) 16
PspGI CCWGG 4 cut(s) 229, 521, 736, 895
PspN4I GGNNCC 2 cut(s) 235, 883
PspPI GGNCC 1 cut(s) 1083
PstNI CAGNNNCTG 1 cut(s) 470
PsuI RGATCY 7 cut(s) 47, 233, 677, 799, 881, 931, 1101
PvuII CAGCTG 2 cut(s) 641, 858
SacI GAGCTC 1 cut(s) 895
SapI GCTCTTC 1 cut(s) 32
SaqAI TTAA 2 cut(s) 201, 695
SatI GCNGC 3 cut(s) 438, 441, 642
Sau96I GGNCC 1 cut(s) 1083
SchI GAGTC 1 cut(s) 529
ScrFI CCNGG 4 cut(s) 231, 523, 738, 897
SduI GDGCHC 5 cut(s) 637, 751, 781, 895, 1058
SexAI ACCWGGT 1 cut(s) 736
SfaNI GCATC 3 cut(s) 501, 978, 1051
SfcI CTRYAG 1 cut(s) 207
SinI GGWCC 1 cut(s) 1083
Sse9I AATT 4 cut(s) 196, 559, 691, 867
SspMI CTAG 4 cut(s) 165, 597, 1080, 1111
SstI GAGCTC 1 cut(s) 895
StyD4I CCNGG 4 cut(s) 229, 521, 736, 895
StyI CCWWGG 5 cut(s) 121, 375, 886, 1079, 1086
TaaI ACNGT 4 cut(s) 160, 288, 716, 776
TaqI TCGA 1 cut(s) 742
TasI AATT 4 cut(s) 196, 559, 691, 867
TfiI GAWTC 6 cut(s) 315, 384, 452, 458, 467, 1025
Tru1I TTAA 2 cut(s) 201, 695
Tru9I TTAA 2 cut(s) 201, 695
TscAI CASTG 5 cut(s) 18, 291, 637, 706, 721
TseFI GTSAC 1 cut(s) 553
TseI GCWGC 3 cut(s) 437, 440, 641
Tsp45I GTSAC 1 cut(s) 553
TspDTI ATGAA 6 cut(s) 291, 429, 735, 929, 1017, 1064
TspGWI ACGGA 2 cut(s) 828, 996
TspRI CASTG 5 cut(s) 18, 291, 637, 706, 721
VpaK11BI GGWCC 1 cut(s) 1083
XagI CCTNNNNNAGG 3 cut(s) 741, 787, 791
XapI RAATTY 1 cut(s) 691
XmaJI CCTAGG 1 cut(s) 1079
XmnI GAANNNNTTC 1 cut(s) 319
XspI CTAG 4 cut(s) 165, 597, 1080, 1111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.