RchiOBHm_Chr2g0109881

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004282
Physical Location & Seq
Reverse (-)
21216340 .. 21221344
5005 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48365

Sequence Viewer

Length: 1119 bp
ATGTCTCCACCACTGCTGGGTGGTGAGGGGGAAGGGAAGAGCAATGGATCGATGGTAGCTTGTTCTCCCTCTATGGACATGATCCCTCAGAACAGCTCAGGACTGAAAGAGCGTAACTACCTTGGGTTGTCAGATTGTTCTTCAGTTGACAGCTCCACTGTCTCAAGCTTGTCAGAGGAGAATAAGAGCAAGTTCAATTTTAAGGCTACAGAGTTGAGGCTTGGTCTTCCTGGATCCCAATCACCAGAACGACAATCAGAGCTTTGCTTGCTTGATGAGAAGCCACTGTTTCCTTTGCTTCCTTCAAAAGATGGAATATGTTCCTCATCCCAGAAAAATGTTGTTTCAGGCAACAAAAGAGGCTTTTCTGATACCATGGACGGATTCTCAGAGGTGAAAAGTTCTGTATATAATGAAGGAAATTGGATGTTTCCTGCAGCTGGTTCTGATTCTGAATCACCAGAGTCTGTCGGACAAGGTAAGTTTCCTGTGAGTTCAATCAATGTGATGCTCTCATCCAGGACTTCTGGGACTCAGCCAACCAAGATGAAAGATATTATGACCAAATCTTCAAAAGATCAGTCCAATGCTACAAATGGAGCTGGCCAGAACCATGCGGCTGCTTCTAACAACAACAGTGCCCCCGCTGCTAAGGCACAAGTTGTAGGGTGGCCTCCAATAAGATCTTTTAGGAAGAATTTAACCACCGCTTCAAAGAACAATGATGAAGTTGATGGAAAACCTGGTCGAGGTGCTCTTTTTGTCAAGGTCAGCATGGATGGTGCTCCCTACCTGAGGAAGGTAGATCTGAGAACCTACTCAACATATCAAGAGTTGTCTTCTGCTCTGGAAAAGATGTTCAGCTGTTTTACCATCGGGCAATATGGATCCCATGGAGCTCCAGGGAGGGAAAGATTGAGTGAGAGTAAGCTGAGAGACCTTCTTCATGGATCAGAATATGTGCTTACATATGAGGACAAGGATGGTGATTGGATGCTTGTCGGAGATGTCCCATGGGAGATGTTTATTGATTCATGCAAGAGGTTGAAGATAATGAAGGGCTCTGATGCCATTGGCTTAGCTCCTAGGTCCATGGAGAAATCCAAGAACCGGACCTAG

Protein Analysis

372

Amino Acids

40.17

Weight (kDa)

8.09

Isoelectric Point (pI)

48.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 69 - 363 4.5e-91 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 617, 645, 708
AclWI GGATC 7 cut(s) 55, 76, 228, 241, 882, 895, 958
AcoI YGGCCR 1 cut(s) 604
AcsI RAATTY 1 cut(s) 697
AcuI CTGAAG 1 cut(s) 126
AfiI CCNNNNNNNGG 6 cut(s) 17, 440, 749, 795, 799, 1110
AgsI TTSAA 6 cut(s) 196, 306, 498, 573, 714, 1048
AjnI CCWGG 4 cut(s) 229, 518, 742, 901
AloI GAACNNNNNNTCC 2 cut(s) 842, 874
Alw21I GWGCWC 3 cut(s) 757, 787, 901
Alw26I GTCTC 3 cut(s) 9, 166, 930
AlwI GGATC 7 cut(s) 55, 76, 228, 241, 882, 895, 958
AlwNI CAGNNNCTG 1 cut(s) 467
AoxI GGCC 2 cut(s) 604, 671
ApeKI GCWGC 3 cut(s) 437, 620, 647
ApoI RAATTY 1 cut(s) 697
ArsI GACNNNNNNTTYG 2 cut(s) 566, 598
Asp700I GAANNNNTTC 1 cut(s) 319
AspA2I CCTAGG 1 cut(s) 1085
AspS9I GGNCC 2 cut(s) 1089, 1113
AsuHPI GGTGA 5 cut(s) 35, 234, 406, 450, 998
AvaII GGWCC 2 cut(s) 1089, 1113
AvrII CCTAGG 1 cut(s) 1085
AxyI CCTNAGG 1 cut(s) 794
BaeGI GKGCMC 1 cut(s) 643
BalI TGGCCA 1 cut(s) 606
BamHI GGATCC 2 cut(s) 233, 887
BanII GRGCYC 2 cut(s) 901, 1064
BbsI GAAGAC 2 cut(s) 218, 831
Bbv12I GWGCWC 3 cut(s) 757, 787, 901
BbvI GCAGC 3 cut(s) 449, 607, 634
BccI CCATC 6 cut(s) 46, 305, 728, 773, 881, 977
BciT130I CCWGG 4 cut(s) 231, 520, 744, 903
BcoDI GTCTC 3 cut(s) 9, 166, 930
BfaI CTAG 2 cut(s) 1086, 1117
BfmI CTRYAG 2 cut(s) 207, 435
BglII AGATCT 2 cut(s) 683, 805
BisI GCNGC 4 cut(s) 438, 618, 621, 648
BlnI CCTAGG 1 cut(s) 1085
BlpI GCTNAGC 1 cut(s) 1078
BlsI GCNGC 4 cut(s) 439, 619, 622, 649
Bme1390I CCNGG 4 cut(s) 231, 520, 744, 903
Bme18I GGWCC 2 cut(s) 1089, 1113
BmgT120I GGNCC 2 cut(s) 1089, 1113
BmiI GGNNCC 2 cut(s) 235, 889
BmrFI CCNGG 4 cut(s) 231, 520, 744, 903
BmsI GCATC 3 cut(s) 498, 984, 1057
BpiI GAAGAC 2 cut(s) 218, 831
BpmI CTGGAG 1 cut(s) 885
Bpu10I CCTNAGC 2 cut(s) 97, 651
Bpu1102I GCTNAGC 1 cut(s) 1078
BpuEI CTTGAG 1 cut(s) 148
Bsa29I ATCGAT 1 cut(s) 50
BsaBI GATNNNNATC 1 cut(s) 238
BsaI GGTCTC 1 cut(s) 930
BsaJI CCNNGG 7 cut(s) 121, 375, 892, 902, 1013, 1085, 1092
BsaWI WCCGGW 1 cut(s) 1110
Bsc4I CCNNNNNNNGG 6 cut(s) 17, 440, 749, 795, 799, 1110
Bse21I CCTNAGG 1 cut(s) 794
Bse3DI GCAATG 1 cut(s) 49
Bse8I GATNNNNATC 1 cut(s) 238
BseBI CCWGG 4 cut(s) 231, 520, 744, 903
BseCI ATCGAT 1 cut(s) 50
BseDI CCNNGG 7 cut(s) 121, 375, 892, 902, 1013, 1085, 1092
BseGI GGATG 6 cut(s) 326, 432, 515, 784, 988, 999
BseJI GATNNNNATC 1 cut(s) 238
BseLI CCNNNNNNNGG 6 cut(s) 17, 440, 749, 795, 799, 1110
BseMI GCAATG 1 cut(s) 49
BseMII CTCAG 7 cut(s) 101, 111, 402, 548, 785, 800, 923
BseRI GAGGAG 1 cut(s) 191
BseSI GKGCMC 1 cut(s) 643
BseXI GCAGC 3 cut(s) 449, 607, 634
BseYI CCCAGC 1 cut(s) 16
BshFI GGCC 2 cut(s) 606, 673
BshVI ATCGAT 1 cut(s) 50
BsiHKAI GWGCWC 3 cut(s) 757, 787, 901
BsiSI CCGG 1 cut(s) 1111
BslFI GGGAC 2 cut(s) 544, 995
BslI CCNNNNNNNGG 6 cut(s) 17, 440, 749, 795, 799, 1110
BsmAI GTCTC 3 cut(s) 9, 166, 930
BsmFI GGGAC 2 cut(s) 544, 995
BsnI GGCC 2 cut(s) 606, 673
Bso31I GGTCTC 1 cut(s) 930
Bsp1286I GDGCHC 5 cut(s) 643, 757, 787, 901, 1064
Bsp143I GATC 8 cut(s) 47, 81, 233, 577, 683, 805, 887, 950
Bsp1720I GCTNAGC 1 cut(s) 1078
Bsp19I CCATGG 4 cut(s) 375, 892, 1013, 1092
BspACI CCGC 3 cut(s) 617, 645, 708
BspANI GGCC 2 cut(s) 606, 673
BspCNI CTCAG 7 cut(s) 100, 110, 401, 547, 786, 801, 924
BspDI ATCGAT 1 cut(s) 50
BspLI GGNNCC 2 cut(s) 235, 889
BspMAI CTGCAG 1 cut(s) 439
BspPI GGATC 7 cut(s) 55, 76, 228, 241, 882, 895, 958
BspQI GCTCTTC 1 cut(s) 32
BspTNI GGTCTC 1 cut(s) 930
BsrDI GCAATG 1 cut(s) 49
BssECI CCNNGG 7 cut(s) 121, 375, 892, 902, 1013, 1085, 1092
BssMI GATC 8 cut(s) 47, 81, 233, 577, 683, 805, 887, 950
BssT1I CCWWGG 6 cut(s) 121, 375, 892, 1013, 1085, 1092
Bst2UI CCWGG 4 cut(s) 231, 520, 744, 903
Bst4CI ACNGT 3 cut(s) 160, 288, 638
Bst6I CTCTTC 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 269, 604
BstDEI CTNAG 9 cut(s) 87, 97, 388, 534, 651, 794, 809, 932, 1078
BstDSI CCRYGG 4 cut(s) 375, 892, 1013, 1092
BstENI CCTNNNNNAGG 3 cut(s) 747, 793, 797
BstF5I GGATG 6 cut(s) 326, 432, 515, 784, 988, 999
BstKTI GATC 8 cut(s) 50, 84, 236, 580, 686, 808, 890, 953
BstMAI GTCTC 3 cut(s) 9, 166, 930
BstMBI GATC 8 cut(s) 47, 81, 233, 577, 683, 805, 887, 950
BstMWI GCNNNNNNNGC 3 cut(s) 268, 647, 653
BstNI CCWGG 4 cut(s) 231, 520, 744, 903
BstSCI CCNGG 4 cut(s) 229, 518, 742, 901
BstSFI CTRYAG 2 cut(s) 207, 435
BstSLI GKGCMC 1 cut(s) 643
BstV1I GCAGC 3 cut(s) 449, 607, 634
BstV2I GAAGAC 2 cut(s) 218, 831
BstX2I RGATCY 4 cut(s) 233, 683, 805, 887
BstYI RGATCY 4 cut(s) 233, 683, 805, 887
Bsu15I ATCGAT 1 cut(s) 50
Bsu36I CCTNAGG 1 cut(s) 794
BsuRI GGCC 2 cut(s) 606, 673
BsuTUI ATCGAT 1 cut(s) 50
BtgI CCRYGG 4 cut(s) 375, 892, 1013, 1092
BtsCI GGATG 6 cut(s) 326, 432, 515, 784, 988, 999
BtsI GCAGTG 1 cut(s) 11
BtsIMutI CAGTG 4 cut(s) 11, 156, 284, 643
Cac8I GCNNGC 2 cut(s) 269, 604
CaiI CAGNNNCTG 1 cut(s) 467
Cfr13I GGNCC 2 cut(s) 1089, 1113
ClaI ATCGAT 1 cut(s) 50
CsiI ACCWGGT 1 cut(s) 742
CviAII CATG 9 cut(s) 79, 376, 614, 775, 893, 947, 1014, 1035, 1093
DdeI CTNAG 9 cut(s) 87, 97, 388, 534, 651, 794, 809, 932, 1078
DpnI GATC 8 cut(s) 49, 83, 235, 579, 685, 807, 889, 952
DpnII GATC 8 cut(s) 47, 81, 233, 577, 683, 805, 887, 950
EaeI YGGCCR 1 cut(s) 604
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 899
Eco130I CCWWGG 6 cut(s) 121, 375, 892, 1013, 1085, 1092
Eco24I GRGCYC 2 cut(s) 901, 1064
Eco31I GGTCTC 1 cut(s) 930
Eco47I GGWCC 2 cut(s) 1089, 1113
Eco53kI GAGCTC 1 cut(s) 899
Eco57I CTGAAG 1 cut(s) 126
Eco81I CCTNAGG 1 cut(s) 794
EcoICRI GAGCTC 1 cut(s) 899
EcoNI CCTNNNNNAGG 3 cut(s) 747, 793, 797
EcoRII CCWGG 4 cut(s) 229, 518, 742, 901
EcoT14I CCWWGG 6 cut(s) 121, 375, 892, 1013, 1085, 1092
EcoT38I GRGCYC 2 cut(s) 901, 1064
ErhI CCWWGG 6 cut(s) 121, 375, 892, 1013, 1085, 1092
FaeI CATG 9 cut(s) 82, 379, 617, 778, 896, 950, 1017, 1038, 1096
FaqI GGGAC 2 cut(s) 544, 995
FatI CATG 9 cut(s) 78, 375, 613, 774, 892, 946, 1013, 1034, 1092
FauI CCCGC 1 cut(s) 652
FauNDI CATATG 1 cut(s) 970
Fnu4HI GCNGC 4 cut(s) 438, 618, 621, 648
FokI GGATG 6 cut(s) 313, 439, 502, 791, 995, 1006
FriOI GRGCYC 2 cut(s) 901, 1064
Fsp4HI GCNGC 4 cut(s) 438, 618, 621, 648
FspBI CTAG 2 cut(s) 1086, 1117
GluI GCNGC 4 cut(s) 438, 618, 621, 648
GsaI CCCAGC 1 cut(s) 20
GsuI CTGGAG 1 cut(s) 885
HaeIII GGCC 2 cut(s) 606, 673
HapII CCGG 1 cut(s) 1111
Hin1II CATG 9 cut(s) 82, 379, 617, 778, 896, 950, 1017, 1038, 1096
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HindIII AAGCTT 1 cut(s) 166
HinfI GANTC 6 cut(s) 384, 449, 455, 464, 532, 1031
HpaII CCGG 1 cut(s) 1111
HphI GGTGA 5 cut(s) 35, 234, 406, 450, 998
Hpy166II GTNNAC 1 cut(s) 148
Hpy188III TCNNGA 3 cut(s) 99, 830, 848
Hpy8I GTNNAC 1 cut(s) 148
HpyAV CCTTC 6 cut(s) 26, 312, 410, 793, 950, 1051
HpyCH4III ACNGT 3 cut(s) 160, 288, 638
HpyCH4V TGCA 2 cut(s) 437, 1038
HpyF10VI GCNNNNNNNGC 3 cut(s) 268, 647, 653
HpyF3I CTNAG 9 cut(s) 87, 97, 388, 534, 651, 794, 809, 932, 1078
Hsp92II CATG 9 cut(s) 82, 379, 617, 778, 896, 950, 1017, 1038, 1096
Kzo9I GATC 8 cut(s) 47, 81, 233, 577, 683, 805, 887, 950
LguI GCTCTTC 1 cut(s) 32
LmnI GCTCC 6 cut(s) 158, 599, 790, 896, 904, 1087
Lsp1109I GCAGC 3 cut(s) 449, 607, 634
LweI GCATC 3 cut(s) 498, 984, 1057
MabI ACCWGGT 1 cut(s) 742
MaeI CTAG 2 cut(s) 1086, 1117
MaeIII GTNAC 1 cut(s) 113
MalI GATC 8 cut(s) 49, 83, 235, 579, 685, 807, 889, 952
MboI GATC 8 cut(s) 47, 81, 233, 577, 683, 805, 887, 950
MboII GAAGA 8 cut(s) 49, 132, 218, 561, 706, 831, 935, 1060
MflI RGATCY 4 cut(s) 233, 683, 805, 887
MhlI GDGCHC 5 cut(s) 643, 757, 787, 901, 1064
MlsI TGGCCA 1 cut(s) 606
MluCI AATT 3 cut(s) 196, 421, 697
MluNI TGGCCA 1 cut(s) 606
MlyI GAGTC 2 cut(s) 473, 526
MmeI TCCRAC 2 cut(s) 451, 982
Mox20I TGGCCA 1 cut(s) 606
MroXI GAANNNNTTC 1 cut(s) 319
MscI TGGCCA 1 cut(s) 606
MseI TTAA 2 cut(s) 201, 701
Msp20I TGGCCA 1 cut(s) 606
MspA1I CMGCKG 3 cut(s) 440, 647, 864
MspI CCGG 1 cut(s) 1111
MspR9I CCNGG 4 cut(s) 231, 520, 744, 903
MvaI CCWGG 4 cut(s) 231, 520, 744, 903
MwoI GCNNNNNNNGC 3 cut(s) 268, 647, 653
NcoI CCATGG 4 cut(s) 375, 892, 1013, 1092
NdeI CATATG 1 cut(s) 970
NdeII GATC 8 cut(s) 47, 81, 233, 577, 683, 805, 887, 950
NlaIII CATG 9 cut(s) 82, 379, 617, 778, 896, 950, 1017, 1038, 1096
NlaIV GGNNCC 2 cut(s) 235, 889
PciSI GCTCTTC 1 cut(s) 32
PdmI GAANNNNTTC 1 cut(s) 319
PfeI GAWTC 4 cut(s) 384, 449, 455, 1031
PfoI TCCNGGA 2 cut(s) 229, 518
PkrI GCNGC 4 cut(s) 439, 619, 622, 649
PleI GAGTC 2 cut(s) 472, 526
PpsI GAGTC 2 cut(s) 472, 526
Psp124BI GAGCTC 1 cut(s) 901
Psp6I CCWGG 4 cut(s) 229, 518, 742, 901
PspFI CCCAGC 1 cut(s) 16
PspGI CCWGG 4 cut(s) 229, 518, 742, 901
PspN4I GGNNCC 2 cut(s) 235, 889
PspPI GGNCC 2 cut(s) 1089, 1113
PstI CTGCAG 1 cut(s) 439
PstNI CAGNNNCTG 1 cut(s) 467
PsuI RGATCY 4 cut(s) 233, 683, 805, 887
PvuII CAGCTG 2 cut(s) 440, 864
SacI GAGCTC 1 cut(s) 901
SapI GCTCTTC 1 cut(s) 32
SaqAI TTAA 2 cut(s) 201, 701
SatI GCNGC 4 cut(s) 438, 618, 621, 648
Sau3AI GATC 8 cut(s) 47, 81, 233, 577, 683, 805, 887, 950
Sau96I GGNCC 2 cut(s) 1089, 1113
SchI GAGTC 2 cut(s) 473, 526
ScrFI CCNGG 4 cut(s) 231, 520, 744, 903
SduI GDGCHC 5 cut(s) 643, 757, 787, 901, 1064
SexAI ACCWGGT 1 cut(s) 742
SfaNI GCATC 3 cut(s) 498, 984, 1057
SfcI CTRYAG 2 cut(s) 207, 435
SinI GGWCC 2 cut(s) 1089, 1113
SmlI CTYRAG 1 cut(s) 163
SmoI CTYRAG 1 cut(s) 163
Sse9I AATT 3 cut(s) 196, 421, 697
SsiI CCGC 3 cut(s) 617, 645, 708
SspMI CTAG 2 cut(s) 1086, 1117
SstI GAGCTC 1 cut(s) 901
StyD4I CCNGG 4 cut(s) 229, 518, 742, 901
StyI CCWWGG 6 cut(s) 121, 375, 892, 1013, 1085, 1092
TaaI ACNGT 3 cut(s) 160, 288, 638
TaqI TCGA 2 cut(s) 50, 748
TasI AATT 3 cut(s) 196, 421, 697
TauI GCSGC 1 cut(s) 620
TfiI GAWTC 4 cut(s) 384, 449, 455, 1031
Tru1I TTAA 2 cut(s) 201, 701
Tru9I TTAA 2 cut(s) 201, 701
TscAI CASTG 4 cut(s) 18, 163, 291, 643
TseI GCWGC 3 cut(s) 437, 620, 647
TspDTI ATGAA 6 cut(s) 429, 563, 741, 935, 1023, 1070
TspGWI ACGGA 1 cut(s) 396
TspRI CASTG 4 cut(s) 18, 163, 291, 643
VpaK11BI GGWCC 2 cut(s) 1089, 1113
XagI CCTNNNNNAGG 3 cut(s) 747, 793, 797
XapI RAATTY 1 cut(s) 697
XmaJI CCTAGG 1 cut(s) 1085
XmnI GAANNNNTTC 1 cut(s) 319
XspI CTAG 2 cut(s) 1086, 1117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.