pycom01g07180

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004282
Physical Location & Seq
Forward (+)
7958447 .. 7961342
2896 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g07180.1

Sequence Viewer

Length: 1071 bp
ATGGTAGCTTTTTCACGCTCTATGGACTGTATCTCTCATAACGGTTCTGGACTGAAAGAACGTAACTACCTAGGATTGTCAGATTGTTCTTCAGTCGACAGCTCCACGGTCTCGAACTTGTCAGAGGGGACTAAGAACAATCTGAACTTTAAGGCTACGGAGTTGAGGCTTGGTCTTCCTGGATCCCAATCACCAGAACGGGAACCTGATCTTTGCTTGTTGAACTCGGGGAAACTTGACGAGAAGCCACTGTTTCCTTTGCTTCCTTCAAAAGATGGAATCTGCTCCTCATCACAGAAGAATGGCAACAAAAGGGGCTTTGCTGACACTATGGATGGATTCTCAGAGGTGAAAAGTAATGCATATACTGAAGGAAATTGGATGTTTCATGCAGCTGGCTCTGATTCTGAATCTCCAGAATCTGTTGGACAAGGGAAGTTTCCTGTGAATTCAATCAATGTAATGCTATCATCCAGGCCTTCTGGGAGTCAGCCAACCATAACAAAAGAAGCACGCACTAAACAAGAACAATCTAATGCTACAAACGGAGGCAGCCATAACCCTTTGGGTGCTTCTAACAATGGAAGTGCCCCAGCTGCTAAGGCACAAGTTGTTGGATGGCCGCCAATTAGATCATTTAGGAAGAATTCTCTAGCCACTACGTCAAAGAACAATGATGAAGTGAATGGAAAACCTGGTCCCGGTGGACTTTTTGTCAAGGTCAGCATGGATGGGGCTCCCTACCTGAGGAAGGTAGACCTCAGAACCTACTCTACATATCAAGATCTGTCTTCTGCTCTGGAAAAGATGTTCAGCTGTTTTACCATAGGGCAATACGGGTCCCATGGAGCTCCGGGGAGGGAAAGACTGAGTGAGAGTAAGCTGAGAGATCTTCTTCACGGATCAGAATATGTGCTTACATATGAGGATAAGGATGGTGACTGGATGCTTGTTGGGGATGTCCCATGGGAGATGTTTATTGACACATGCAAGAGGTTGAAGATAATGAAGGGCTCCGATGCCATTGGTTTAGCTCCTAGGGCCATGGAGAAATCCAAGAACCAGAACTAG

Protein Analysis

357

Amino Acids

38.5

Weight (kDa)

8.11

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 52 - 347 5.4e-90 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 96, 756
AciI CCGC 1 cut(s) 623
AclWI GGATC 3 cut(s) 177, 190, 910
AcoI YGGCCR 1 cut(s) 620
AcsI RAATTY 2 cut(s) 448, 646
AcuI CTGAAG 2 cut(s) 75, 390
AfiI CCNNNNNNNGG 3 cut(s) 701, 747, 751
AgsI TTSAA 4 cut(s) 223, 270, 453, 1000
AhdI GACNNNNNGTC 1 cut(s) 713
AjnI CCWGG 3 cut(s) 178, 473, 694
AloI GAACNNNNNNTCC 2 cut(s) 794, 826
AluBI AGCT 8 cut(s) 8, 102, 395, 596, 816, 851, 883, 1034
AluI AGCT 8 cut(s) 8, 102, 395, 596, 816, 851, 883, 1034
Alw21I GWGCWC 1 cut(s) 853
Alw26I GTCTC 1 cut(s) 115
AlwI GGATC 3 cut(s) 177, 190, 910
AlwNI CAGNNNCTG 1 cut(s) 422
Ama87I CYCGRG 1 cut(s) 226
AoxI GGCC 3 cut(s) 476, 620, 1041
ApeKI GCWGC 3 cut(s) 392, 552, 596
ApoI RAATTY 2 cut(s) 448, 646
AspA2I CCTAGG 2 cut(s) 70, 1037
AspS9I GGNCC 3 cut(s) 698, 840, 1041
AsuC2I CCSGG 2 cut(s) 702, 855
AsuHPI GGTGA 3 cut(s) 183, 361, 950
AvaI CYCGRG 1 cut(s) 226
AvaII GGWCC 2 cut(s) 698, 840
AvrII CCTAGG 2 cut(s) 70, 1037
AxyI CCTNAGG 1 cut(s) 746
BaeGI GKGCMC 1 cut(s) 592
BamHI GGATCC 1 cut(s) 182
BanII GRGCYC 3 cut(s) 739, 853, 1016
BbsI GAAGAC 2 cut(s) 167, 783
Bbv12I GWGCWC 1 cut(s) 853
BbvI GCAGC 3 cut(s) 404, 564, 583
BccI CCATC 5 cut(s) 269, 329, 612, 725, 929
BciT130I CCWGG 3 cut(s) 180, 475, 696
BcnI CCSGG 2 cut(s) 702, 855
BcoDI GTCTC 1 cut(s) 115
BfaI CTAG 4 cut(s) 71, 653, 1038, 1069
BglII AGATCT 2 cut(s) 784, 889
BisI GCNGC 4 cut(s) 393, 553, 597, 623
BlnI CCTAGG 2 cut(s) 70, 1037
BlsI GCNGC 4 cut(s) 394, 554, 598, 624
Bme1390I CCNGG 5 cut(s) 180, 475, 696, 702, 855
Bme18I GGWCC 2 cut(s) 698, 840
BmeRI GACNNNNNGTC 1 cut(s) 713
BmeT110I CYCGRG 1 cut(s) 226
BmgT120I GGNCC 3 cut(s) 698, 840, 1041
BmiI GGNNCC 7 cut(s) 184, 204, 700, 738, 841, 842, 1015
BmrFI CCNGG 5 cut(s) 180, 475, 696, 702, 855
BmsI GCATC 2 cut(s) 936, 1009
BpiI GAAGAC 2 cut(s) 167, 783
BpmI CTGGAG 1 cut(s) 399
Bpu10I CCTNAGC 1 cut(s) 600
BpuMI CCSGG 2 cut(s) 702, 855
BsaBI GATNNNNATC 1 cut(s) 187
BsaI GGTCTC 1 cut(s) 115
BsaJI CCNNGG 7 cut(s) 70, 105, 844, 854, 965, 1037, 1044
Bsc4I CCNNNNNNNGG 3 cut(s) 701, 747, 751
Bse1I ACTGG 1 cut(s) 947
Bse21I CCTNAGG 1 cut(s) 746
Bse8I GATNNNNATC 1 cut(s) 187
BseBI CCWGG 3 cut(s) 180, 475, 696
BseDI CCNNGG 7 cut(s) 70, 105, 844, 854, 965, 1037, 1044
BseGI GGATG 8 cut(s) 340, 387, 470, 623, 736, 940, 951, 964
BseJI GATNNNNATC 1 cut(s) 187
BseLI CCNNNNNNNGG 3 cut(s) 701, 747, 751
BseMII CTCAG 5 cut(s) 357, 737, 775, 860, 875
BseNI ACTGG 1 cut(s) 947
BseRI GAGGAG 1 cut(s) 277
BseSI GKGCMC 1 cut(s) 592
BseXI GCAGC 3 cut(s) 404, 564, 583
BseYI CCCAGC 1 cut(s) 592
BshFI GGCC 3 cut(s) 478, 622, 1043
BsiHKAI GWGCWC 1 cut(s) 853
BsiHKCI CYCGRG 1 cut(s) 226
BsiSI CCGG 2 cut(s) 702, 854
BslFI GGGAC 4 cut(s) 142, 684, 826, 947
BslI CCNNNNNNNGG 3 cut(s) 701, 747, 751
BsmAI GTCTC 1 cut(s) 115
BsmFI GGGAC 4 cut(s) 142, 684, 826, 947
BsnI GGCC 3 cut(s) 478, 622, 1043
Bso31I GGTCTC 1 cut(s) 115
BsoBI CYCGRG 1 cut(s) 226
Bsp1286I GDGCHC 4 cut(s) 592, 739, 853, 1016
Bsp143I GATC 6 cut(s) 182, 208, 632, 784, 889, 902
Bsp19I CCATGG 3 cut(s) 844, 965, 1044
BspACI CCGC 1 cut(s) 623
BspANI GGCC 3 cut(s) 478, 622, 1043
BspCNI CTCAG 5 cut(s) 356, 738, 774, 861, 876
BspLI GGNNCC 7 cut(s) 184, 204, 700, 738, 841, 842, 1015
BspPI GGATC 3 cut(s) 177, 190, 910
BspTNI GGTCTC 1 cut(s) 115
BsrI ACTGG 1 cut(s) 947
BssECI CCNNGG 7 cut(s) 70, 105, 844, 854, 965, 1037, 1044
BssMI GATC 6 cut(s) 182, 208, 632, 784, 889, 902
BssT1I CCWWGG 5 cut(s) 70, 844, 965, 1037, 1044
Bst2UI CCWGG 3 cut(s) 180, 475, 696
Bst4CI ACNGT 4 cut(s) 29, 44, 109, 252
BstC8I GCNNGC 2 cut(s) 397, 514
BstDEI CTNAG 7 cut(s) 132, 343, 600, 746, 761, 869, 884
BstDSI CCRYGG 4 cut(s) 105, 844, 965, 1044
BstENI CCTNNNNNAGG 2 cut(s) 745, 749
BstF5I GGATG 8 cut(s) 340, 387, 470, 623, 736, 940, 951, 964
BstKTI GATC 6 cut(s) 185, 211, 635, 787, 892, 905
BstMAI GTCTC 1 cut(s) 115
BstMBI GATC 6 cut(s) 182, 208, 632, 784, 889, 902
BstMWI GCNNNNNNNGC 3 cut(s) 596, 602, 1040
BstNI CCWGG 3 cut(s) 180, 475, 696
BstNSI RCATGY 1 cut(s) 990
BstSCI CCNGG 5 cut(s) 178, 473, 694, 700, 853
BstSLI GKGCMC 1 cut(s) 592
BstV1I GCAGC 3 cut(s) 404, 564, 583
BstV2I GAAGAC 2 cut(s) 167, 783
BstX2I RGATCY 3 cut(s) 182, 784, 889
BstYI RGATCY 3 cut(s) 182, 784, 889
Bsu36I CCTNAGG 1 cut(s) 746
BsuRI GGCC 3 cut(s) 478, 622, 1043
BtgI CCRYGG 4 cut(s) 105, 844, 965, 1044
BtsCI GGATG 8 cut(s) 340, 387, 470, 623, 736, 940, 951, 964
BtsIMutI CAGTG 1 cut(s) 248
Cac8I GCNNGC 2 cut(s) 397, 514
CaiI CAGNNNCTG 1 cut(s) 422
Cfr13I GGNCC 3 cut(s) 698, 840, 1041
CsiI ACCWGGT 1 cut(s) 694
CviAII CATG 6 cut(s) 389, 727, 845, 966, 987, 1045
DdeI CTNAG 7 cut(s) 132, 343, 600, 746, 761, 869, 884
DpnI GATC 6 cut(s) 184, 210, 634, 786, 891, 904
DpnII GATC 6 cut(s) 182, 208, 632, 784, 889, 902
DriI GACNNNNNGTC 1 cut(s) 713
EaeI YGGCCR 1 cut(s) 620
Eam1105I GACNNNNNGTC 1 cut(s) 713
Ecl136II GAGCTC 1 cut(s) 851
Eco130I CCWWGG 5 cut(s) 70, 844, 965, 1037, 1044
Eco147I AGGCCT 1 cut(s) 478
Eco24I GRGCYC 3 cut(s) 739, 853, 1016
Eco31I GGTCTC 1 cut(s) 115
Eco47I GGWCC 2 cut(s) 698, 840
Eco53kI GAGCTC 1 cut(s) 851
Eco57I CTGAAG 2 cut(s) 75, 390
Eco81I CCTNAGG 1 cut(s) 746
Eco88I CYCGRG 1 cut(s) 226
EcoICRI GAGCTC 1 cut(s) 851
EcoNI CCTNNNNNAGG 2 cut(s) 745, 749
EcoO109I RGGNCCY 1 cut(s) 840
EcoRI GAATTC 2 cut(s) 448, 646
EcoRII CCWGG 3 cut(s) 178, 473, 694
EcoT14I CCWWGG 5 cut(s) 70, 844, 965, 1037, 1044
EcoT22I ATGCAT 1 cut(s) 364
EcoT38I GRGCYC 3 cut(s) 739, 853, 1016
ErhI CCWWGG 5 cut(s) 70, 844, 965, 1037, 1044
FaeI CATG 6 cut(s) 392, 730, 848, 969, 990, 1048
FaqI GGGAC 4 cut(s) 142, 684, 826, 947
FatI CATG 6 cut(s) 388, 726, 844, 965, 986, 1044
FauNDI CATATG 1 cut(s) 922
FblI GTMKAC 2 cut(s) 96, 756
Fnu4HI GCNGC 4 cut(s) 393, 553, 597, 623
FokI GGATG 8 cut(s) 347, 394, 457, 630, 743, 947, 958, 971
FriOI GRGCYC 3 cut(s) 739, 853, 1016
Fsp4HI GCNGC 4 cut(s) 393, 553, 597, 623
FspBI CTAG 4 cut(s) 71, 653, 1038, 1069
GluI GCNGC 4 cut(s) 393, 553, 597, 623
GsaI CCCAGC 1 cut(s) 596
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 3 cut(s) 478, 622, 1043
HapII CCGG 2 cut(s) 702, 854
Hin1II CATG 6 cut(s) 392, 730, 848, 969, 990, 1048
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 6 cut(s) 279, 339, 404, 410, 419, 487
HpaII CCGG 2 cut(s) 702, 854
HphI GGTGA 3 cut(s) 183, 361, 950
Hpy166II GTNNAC 3 cut(s) 97, 707, 757
Hpy188I TCNGA 9 cut(s) 82, 124, 144, 346, 403, 409, 764, 907, 1018
Hpy188III TCNNGA 5 cut(s) 48, 112, 416, 782, 800
Hpy8I GTNNAC 3 cut(s) 97, 707, 757
HpyAV CCTTC 5 cut(s) 276, 365, 489, 745, 1003
HpyCH4III ACNGT 4 cut(s) 29, 44, 109, 252
HpyCH4IV ACGT 2 cut(s) 61, 662
HpyCH4V TGCA 3 cut(s) 362, 392, 990
HpyF10VI GCNNNNNNNGC 3 cut(s) 596, 602, 1040
HpyF3I CTNAG 7 cut(s) 132, 343, 600, 746, 761, 869, 884
HpySE526I ACGT 2 cut(s) 61, 662
Hsp92II CATG 6 cut(s) 392, 730, 848, 969, 990, 1048
KflI GGGWCCC 1 cut(s) 840
Kzo9I GATC 6 cut(s) 182, 208, 632, 784, 889, 902
LmnI GCTCC 7 cut(s) 107, 290, 742, 848, 856, 1019, 1039
Lsp1109I GCAGC 3 cut(s) 404, 564, 583
LweI GCATC 2 cut(s) 936, 1009
MabI ACCWGGT 1 cut(s) 694
MaeI CTAG 4 cut(s) 71, 653, 1038, 1069
MaeII ACGT 2 cut(s) 61, 662
MaeIII GTNAC 2 cut(s) 62, 938
MalI GATC 6 cut(s) 184, 210, 634, 786, 891, 904
MboI GATC 6 cut(s) 182, 208, 632, 784, 889, 902
MboII GAAGA 8 cut(s) 81, 167, 310, 655, 783, 884, 887, 1012
MflI RGATCY 3 cut(s) 182, 784, 889
MhlI GDGCHC 4 cut(s) 592, 739, 853, 1016
MluCI AATT 4 cut(s) 376, 448, 627, 646
MlyI GAGTC 1 cut(s) 496
MmeI TCCRAC 2 cut(s) 406, 595
Mph1103I ATGCAT 1 cut(s) 364
MseI TTAA 1 cut(s) 150
MspA1I CMGCKG 3 cut(s) 395, 596, 816
MspI CCGG 2 cut(s) 702, 854
MspR9I CCNGG 5 cut(s) 180, 475, 696, 702, 855
MvaI CCWGG 3 cut(s) 180, 475, 696
MwoI GCNNNNNNNGC 3 cut(s) 596, 602, 1040
NciI CCSGG 2 cut(s) 702, 855
NcoI CCATGG 3 cut(s) 844, 965, 1044
NdeI CATATG 1 cut(s) 922
NdeII GATC 6 cut(s) 182, 208, 632, 784, 889, 902
NlaIII CATG 6 cut(s) 392, 730, 848, 969, 990, 1048
NlaIV GGNNCC 7 cut(s) 184, 204, 700, 738, 841, 842, 1015
NmuCI GTSAC 1 cut(s) 938
NsiI ATGCAT 1 cut(s) 364
NspI RCATGY 1 cut(s) 990
PceI AGGCCT 1 cut(s) 478
PfeI GAWTC 5 cut(s) 279, 339, 404, 410, 419
PfoI TCCNGGA 1 cut(s) 178
PkrI GCNGC 4 cut(s) 394, 554, 598, 624
PleI GAGTC 1 cut(s) 495
PpsI GAGTC 1 cut(s) 495
PpuMI RGGWCCY 1 cut(s) 840
Psp124BI GAGCTC 1 cut(s) 853
Psp5II RGGWCCY 1 cut(s) 840
Psp6I CCWGG 3 cut(s) 178, 473, 694
PspFI CCCAGC 1 cut(s) 592
PspGI CCWGG 3 cut(s) 178, 473, 694
PspN4I GGNNCC 7 cut(s) 184, 204, 700, 738, 841, 842, 1015
PspPI GGNCC 3 cut(s) 698, 840, 1041
PspPPI RGGWCCY 1 cut(s) 840
PsrI GAACNNNNNNTAC 2 cut(s) 757, 789
PstNI CAGNNNCTG 1 cut(s) 422
PsuI RGATCY 3 cut(s) 182, 784, 889
PvuII CAGCTG 3 cut(s) 395, 596, 816
SacI GAGCTC 1 cut(s) 853
SalI GTCGAC 1 cut(s) 95
SaqAI TTAA 1 cut(s) 150
SatI GCNGC 4 cut(s) 393, 553, 597, 623
Sau3AI GATC 6 cut(s) 182, 208, 632, 784, 889, 902
Sau96I GGNCC 3 cut(s) 698, 840, 1041
SchI GAGTC 1 cut(s) 496
ScrFI CCNGG 5 cut(s) 180, 475, 696, 702, 855
SduI GDGCHC 4 cut(s) 592, 739, 853, 1016
SexAI ACCWGGT 1 cut(s) 694
SfaNI GCATC 2 cut(s) 936, 1009
SinI GGWCC 2 cut(s) 698, 840
Sse9I AATT 4 cut(s) 376, 448, 627, 646
SseBI AGGCCT 1 cut(s) 478
SsiI CCGC 1 cut(s) 623
SspMI CTAG 4 cut(s) 71, 653, 1038, 1069
SstI GAGCTC 1 cut(s) 853
StuI AGGCCT 1 cut(s) 478
StyD4I CCNGG 5 cut(s) 178, 473, 694, 700, 853
StyI CCWWGG 5 cut(s) 70, 844, 965, 1037, 1044
TaaI ACNGT 4 cut(s) 29, 44, 109, 252
TaiI ACGT 2 cut(s) 64, 665
TaqI TCGA 2 cut(s) 96, 113
TasI AATT 4 cut(s) 376, 448, 627, 646
TauI GCSGC 1 cut(s) 625
TfiI GAWTC 5 cut(s) 279, 339, 404, 410, 419
Tru1I TTAA 1 cut(s) 150
Tru9I TTAA 1 cut(s) 150
TscAI CASTG 1 cut(s) 255
TseFI GTSAC 1 cut(s) 938
TseI GCWGC 3 cut(s) 392, 552, 596
Tsp45I GTSAC 1 cut(s) 938
TspDTI ATGAA 3 cut(s) 377, 693, 1022
TspGWI ACGGA 3 cut(s) 173, 561, 915
TspRI CASTG 1 cut(s) 255
VpaK11BI GGWCC 2 cut(s) 698, 840
XagI CCTNNNNNAGG 2 cut(s) 745, 749
XapI RAATTY 2 cut(s) 448, 646
XceI RCATGY 1 cut(s) 990
XmaJI CCTAGG 2 cut(s) 70, 1037
XmiI GTMKAC 2 cut(s) 96, 756
XspI CTAG 4 cut(s) 71, 653, 1038, 1069
Zsp2I ATGCAT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.