FvH4_1g20301

acetyltransferase component of pyruvate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0018355
Physical Location & Seq
Reverse (-)
12531076 .. 12531570
495 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g20301.t1

Sequence Viewer

Length: 495 bp
ATGGCCAAGTATAAAATTCCTTGGATTCTGAAATGGCAATACAAAATTGAACTTGAAGAATACATGGTTACAAGACAATTCCTAGTAAAATGGTGGGATAAATTCGACCCTGAGAGAGTTTTAACTCAATTCAGTGAAGAATTCACAGTTATTCCCTCGAGTTCAAAAGCTCCGATTTCCATTCAACCGGCCCCTCCAATCTCGCAGTTAACATCTCAAACTCCTCAGTCACCATCTTCTTCCAAGAAGACAAATCAGTCACCATCCTCTTCTCAGAAGAAAAAGGCCTCCAGTTCATCTTCATCCTCCAAAGCAGAAGAGTTAAAAGAACTAGCAAAGCAGTTACTACAAGAAGCAGCAAATTTATCTACAGATGATGACGAAGAACATTCTCCATCAGAATCATCATCACACAGTCATCAAAGTTCCCCCAATCAACCAAAACGATGGGTAGATTATGAAGATAGTCAAGACCCTTATGCCGAGTTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

165

Amino Acids

18.72

Weight (kDa)

5.29

Isoelectric Point (pI)

91.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 256
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 4 cut(s) 15, 101, 140, 361
AgsI TTSAA 4 cut(s) 50, 56, 165, 185
AluBI AGCT 1 cut(s) 170
AluI AGCT 1 cut(s) 170
Ama87I CYCGRG 1 cut(s) 157
AoxI GGCC 3 cut(s) 3, 189, 285
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 4 cut(s) 15, 101, 140, 361
AspS9I GGNCC 1 cut(s) 190
AsuHPI GGTGA 2 cut(s) 222, 252
AvaI CYCGRG 1 cut(s) 157
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 254
BbvI GCAGC 1 cut(s) 368
BccI CCATC 4 cut(s) 241, 271, 403, 441
BfaI CTAG 2 cut(s) 83, 332
BfmI CTRYAG 1 cut(s) 369
BisI GCNGC 1 cut(s) 357
BlsI GCNGC 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 190
BmiI GGNNCC 1 cut(s) 192
BpiI GAAGAC 1 cut(s) 254
BpmI CTGGAG 1 cut(s) 274
BsaJI CCNNGG 1 cut(s) 20
Bse118I RCCGGY 1 cut(s) 187
Bse1I ACTGG 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 20
BseGI GGATG 2 cut(s) 263, 302
BseMII CTCAG 3 cut(s) 102, 239, 287
BseNI ACTGG 1 cut(s) 291
BseRI GAGGAG 1 cut(s) 213
BseXI GCAGC 1 cut(s) 368
BshFI GGCC 3 cut(s) 5, 191, 287
BsiHKCI CYCGRG 1 cut(s) 157
BsiSI CCGG 1 cut(s) 188
BsnI GGCC 3 cut(s) 5, 191, 287
BsoBI CYCGRG 1 cut(s) 157
BspANI GGCC 3 cut(s) 5, 191, 287
BspCNI CTCAG 3 cut(s) 103, 238, 286
BspLI GGNNCC 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 187
BsrI ACTGG 1 cut(s) 291
BssAI RCCGGY 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 20
BssT1I CCWWGG 1 cut(s) 20
Bst4CI ACNGT 2 cut(s) 148, 416
Bst6I CTCTTC 2 cut(s) 274, 312
BstDEI CTNAG 3 cut(s) 111, 225, 273
BstF5I GGATG 2 cut(s) 263, 302
BstSFI CTRYAG 1 cut(s) 369
BstV1I GCAGC 1 cut(s) 368
BstV2I GAAGAC 1 cut(s) 254
BstXI CCANNNNNNTGG 1 cut(s) 447
BsuRI GGCC 3 cut(s) 5, 191, 287
BtsCI GGATG 2 cut(s) 263, 302
BtsIMutI CAGTG 1 cut(s) 139
Cfr10I RCCGGY 1 cut(s) 187
Cfr13I GGNCC 1 cut(s) 190
CviAII CATG 1 cut(s) 64
CviJI RGCY 4 cut(s) 5, 170, 191, 287
CviKI_1 RGCY 4 cut(s) 5, 170, 191, 287
DdeI CTNAG 3 cut(s) 111, 225, 273
DrdI GACNNNNNNGTC 1 cut(s) 256
DseDI GACNNNNNNGTC 1 cut(s) 256
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 274, 312
EarI CTCTTC 2 cut(s) 274, 312
Eco130I CCWWGG 1 cut(s) 20
Eco147I AGGCCT 1 cut(s) 287
Eco88I CYCGRG 1 cut(s) 157
EcoRI GAATTC 1 cut(s) 140
EcoT14I CCWWGG 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 20
FaeI CATG 1 cut(s) 67
FaiI YATR 4 cut(s) 12, 65, 459, 480
FatI CATG 1 cut(s) 63
Fnu4HI GCNGC 1 cut(s) 357
FokI GGATG 2 cut(s) 250, 289
Fsp4HI GCNGC 1 cut(s) 357
FspBI CTAG 2 cut(s) 83, 332
GluI GCNGC 1 cut(s) 357
GsuI CTGGAG 1 cut(s) 274
HaeIII GGCC 3 cut(s) 5, 191, 287
HapII CCGG 1 cut(s) 188
Hin1II CATG 1 cut(s) 67
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HinfI GANTC 2 cut(s) 25, 401
HpaI GTTAAC 1 cut(s) 210
HpaII CCGG 1 cut(s) 188
HphI GGTGA 2 cut(s) 222, 252
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 4 cut(s) 30, 174, 276, 400
Hpy188III TCNNGA 1 cut(s) 470
Hpy8I GTNNAC 1 cut(s) 210
HpyCH4III ACNGT 2 cut(s) 148, 416
HpyF3I CTNAG 3 cut(s) 111, 225, 273
Hsp92II CATG 1 cut(s) 67
KspAI GTTAAC 1 cut(s) 210
LmnI GCTCC 1 cut(s) 175
LpnPI CCDG 3 cut(s) 123, 201, 304
Lsp1109I GCAGC 1 cut(s) 368
MaeI CTAG 2 cut(s) 83, 332
MaeIII GTNAC 4 cut(s) 67, 228, 258, 342
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 15, 45, 77, 101, 128, 140, 361
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 6 cut(s) 166, 204, 234, 277, 298, 316
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 122, 209, 323, 493
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 188
NlaIII CATG 1 cut(s) 67
NlaIV GGNNCC 1 cut(s) 192
NmuCI GTSAC 2 cut(s) 228, 258
PaeR7I CTCGAG 1 cut(s) 157
PceI AGGCCT 1 cut(s) 287
PfeI GAWTC 2 cut(s) 25, 401
PkrI GCNGC 1 cut(s) 358
PspN4I GGNNCC 1 cut(s) 192
PspPI GGNCC 1 cut(s) 190
PspXI VCTCGAGB 1 cut(s) 157
SaqAI TTAA 4 cut(s) 122, 209, 323, 493
SatI GCNGC 1 cut(s) 357
Sau96I GGNCC 1 cut(s) 190
SetI ASST 1 cut(s) 172
SfcI CTRYAG 1 cut(s) 369
Sfr274I CTCGAG 1 cut(s) 157
SlaI CTCGAG 1 cut(s) 157
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
Sse9I AATT 7 cut(s) 15, 45, 77, 101, 128, 140, 361
SseBI AGGCCT 1 cut(s) 287
SspMI CTAG 2 cut(s) 83, 332
StuI AGGCCT 1 cut(s) 287
StyI CCWWGG 1 cut(s) 20
TaaI ACNGT 2 cut(s) 148, 416
TaqI TCGA 2 cut(s) 105, 158
TasI AATT 7 cut(s) 15, 45, 77, 101, 128, 140, 361
TfiI GAWTC 2 cut(s) 25, 401
Tru1I TTAA 4 cut(s) 122, 209, 323, 493
Tru9I TTAA 4 cut(s) 122, 209, 323, 493
TscAI CASTG 1 cut(s) 139
TseFI GTSAC 2 cut(s) 228, 258
TseI GCWGC 1 cut(s) 356
Tsp45I GTSAC 2 cut(s) 228, 258
TspDTI ATGAA 3 cut(s) 285, 291, 474
TspRI CASTG 1 cut(s) 139
XapI RAATTY 4 cut(s) 15, 101, 140, 361
XhoI CTCGAG 1 cut(s) 157
XspI CTAG 2 cut(s) 83, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.