Rmu_sc0000235.1_g000029

Zinc knuckle family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000235.1
OG0018355
Physical Location & Seq
Forward (+)
80516 .. 81526
1011 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000235.1_g000029.1.cds

Sequence Viewer

Length: 1011 bp
atgagtcatctcaaaaacccaatgtccgtagttaaaacttattaccctcctagttttcactttctacctagtcaccctaccaagtcattaaaattttataaagatattctttttgaaaccaaatcagtcaccattaagcccattcatgacacaaaggatccaactaagatcctataccattctctatacatagtccgtatcatttctctttcccaatggggtgatcctccgttcaccaatcacaaatgcggagacctatcttttaattattatgattatgaagcagcctggaattcaatcttccttcaccaaacagatgatttcagtcattcctggttcttaaattttgataaaatattcaatcgccaattcccaatgtggttccttagatggtggagggctcatggatgccctgacatccttccacaagaaataaaaaatgcagtcacctgtttctcaacattcatgaatttcacaaattatgacttgaattttccaatcacattacactttatggccaaatacaagctcccctggatcttcaaatggcattacgaagtcaaagaacatgtagtctccagacacttcctagtcaaatggtgggataaattcaaaactgaaagaattatctcccaatttaatgaagaatttccaatcactccatcacaacctactcagtcaagagctccagtcactccaatgccaccccaaacggcatccacttcccagtcaaaacctaagtccctcaagtcacattcctcttccaaatccaaggggaaagcaaagtcaacaaaatcagatacatcctccaagtcagaagagttgaaagagttagcaaaaaggctcttagcacaagtagctgatctcagtaatgatgatgatgaatctgaagaagaagaaatttcttcccgatcctcatcatcatctcgcagtcatccaggcactcccggtaaaccagtcaaatgggctgatttccaagacagtcaagacccatatgaagactacgagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP

Protein Analysis

336

Amino Acids

39.37

Weight (kDa)

8.49

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 99
AciI CCGC 1 cut(s) 249
AclWI GGATC 6 cut(s) 152, 163, 165, 218, 545, 906
AcoI YGGCCR 1 cut(s) 516
AcsI RAATTY 8 cut(s) 92, 292, 343, 469, 490, 608, 647, 900
AcuI CTGAAG 1 cut(s) 909
AflIII ACRYGT 1 cut(s) 568
AgsI TTSAA 7 cut(s) 116, 297, 361, 490, 544, 613, 826
AjnI CCWGG 4 cut(s) 287, 332, 533, 937
AluBI AGCT 3 cut(s) 529, 686, 860
AluI AGCT 3 cut(s) 529, 686, 860
Alw21I GWGCWC 1 cut(s) 688
Alw26I GTCTC 2 cut(s) 246, 580
AlwI GGATC 6 cut(s) 152, 163, 165, 218, 545, 906
AoxI GGCC 1 cut(s) 516
ApeKI GCWGC 1 cut(s) 284
ApoI RAATTY 8 cut(s) 92, 292, 343, 469, 490, 608, 647, 900
ArsI GACNNNNNNTTYG 2 cut(s) 725, 757
Asp700I GAANNNNTTC 1 cut(s) 648
AsuC2I CCSGG 1 cut(s) 948
AsuHPI GGTGA 6 cut(s) 65, 121, 226, 233, 299, 439
BalI TGGCCA 1 cut(s) 518
BamHI GGATCC 1 cut(s) 157
BanII GRGCYC 2 cut(s) 403, 688
BbsI GAAGAC 1 cut(s) 1005
Bbv12I GWGCWC 1 cut(s) 688
BbvI GCAGC 1 cut(s) 296
BccI CCATC 2 cut(s) 384, 670
BceAI ACGGC 1 cut(s) 729
BciT130I CCWGG 4 cut(s) 289, 334, 535, 939
BcnI CCSGG 1 cut(s) 948
BcoDI GTCTC 2 cut(s) 246, 580
BfaI CTAG 3 cut(s) 51, 69, 590
BisI GCNGC 1 cut(s) 285
BlsI GCNGC 1 cut(s) 286
Bme1390I CCNGG 5 cut(s) 289, 334, 535, 939, 948
BmiI GGNNCC 2 cut(s) 159, 383
BmrFI CCNGG 5 cut(s) 289, 334, 535, 939, 948
BmrI ACTGGG 1 cut(s) 721
BmsI GCATC 2 cut(s) 398, 725
BmuI ACTGGG 1 cut(s) 721
BpiI GAAGAC 1 cut(s) 1005
BpmI CTGGAG 2 cut(s) 562, 672
BpuEI CTTGAG 1 cut(s) 731
BpuMI CCSGG 1 cut(s) 948
BsaBI GATNNNNATC 1 cut(s) 916
BsaI GGTCTC 1 cut(s) 246
BsaJI CCNNGG 2 cut(s) 533, 771
Bse1I ACTGG 3 cut(s) 689, 727, 956
Bse8I GATNNNNATC 1 cut(s) 916
BseBI CCWGG 4 cut(s) 289, 334, 535, 939
BseDI CCNNGG 2 cut(s) 533, 771
BseGI GGATG 5 cut(s) 413, 417, 716, 803, 934
BseJI GATNNNNATC 1 cut(s) 916
BseMII CTCAG 2 cut(s) 689, 880
BseNI ACTGG 3 cut(s) 689, 727, 956
BseXI GCAGC 1 cut(s) 296
BshFI GGCC 1 cut(s) 518
BsiHKAI GWGCWC 1 cut(s) 688
BsiSI CCGG 1 cut(s) 948
BslFI GGGAC 1 cut(s) 727
BsmAI GTCTC 2 cut(s) 246, 580
BsmFI GGGAC 1 cut(s) 727
BsnI GGCC 1 cut(s) 518
Bso31I GGTCTC 1 cut(s) 246
Bsp1286I GDGCHC 2 cut(s) 403, 688
Bsp143I GATC 6 cut(s) 157, 168, 223, 537, 862, 911
BspACI CCGC 1 cut(s) 249
BspANI GGCC 1 cut(s) 518
BspCNI CTCAG 2 cut(s) 688, 879
BspHI TCATGA 2 cut(s) 145, 465
BspLI GGNNCC 2 cut(s) 159, 383
BspPI GGATC 6 cut(s) 152, 163, 165, 218, 545, 906
BspTNI GGTCTC 1 cut(s) 246
BsrI ACTGG 3 cut(s) 689, 727, 956
BssECI CCNNGG 2 cut(s) 533, 771
BssMI GATC 6 cut(s) 157, 168, 223, 537, 862, 911
BssT1I CCWWGG 1 cut(s) 771
Bst2UI CCWGG 4 cut(s) 289, 334, 535, 939
Bst4CI ACNGT 1 cut(s) 983
Bst6I CTCTTC 2 cut(s) 766, 813
BstDEI CTNAG 6 cut(s) 165, 386, 675, 738, 847, 866
BstF5I GGATG 5 cut(s) 413, 417, 716, 803, 934
BstKTI GATC 6 cut(s) 160, 171, 226, 540, 865, 914
BstMAI GTCTC 2 cut(s) 246, 580
BstMBI GATC 6 cut(s) 157, 168, 223, 537, 862, 911
BstMWI GCNNNNNNNGC 1 cut(s) 857
BstNI CCWGG 4 cut(s) 289, 334, 535, 939
BstNSI RCATGY 1 cut(s) 572
BstSCI CCNGG 5 cut(s) 287, 332, 533, 937, 946
BstV1I GCAGC 1 cut(s) 296
BstV2I GAAGAC 1 cut(s) 1005
BstX2I RGATCY 3 cut(s) 157, 168, 537
BstXI CCANNNNNNTGG 1 cut(s) 963
BstYI RGATCY 3 cut(s) 157, 168, 537
BsuRI GGCC 1 cut(s) 518
BtsCI GGATG 5 cut(s) 413, 417, 716, 803, 934
CciI TCATGA 2 cut(s) 145, 465
CviAII CATG 4 cut(s) 146, 404, 466, 569
CviJI RGCY 9 cut(s) 139, 287, 401, 518, 529, 686, 844, 860, 968
CviKI_1 RGCY 9 cut(s) 139, 287, 401, 518, 529, 686, 844, 860, 968
DdeI CTNAG 6 cut(s) 165, 386, 675, 738, 847, 866
DpnI GATC 6 cut(s) 159, 170, 225, 539, 864, 913
DpnII GATC 6 cut(s) 157, 168, 223, 537, 862, 911
EaeI YGGCCR 1 cut(s) 516
Eam1104I CTCTTC 2 cut(s) 766, 813
EarI CTCTTC 2 cut(s) 766, 813
Ecl136II GAGCTC 1 cut(s) 686
Eco130I CCWWGG 1 cut(s) 771
Eco24I GRGCYC 2 cut(s) 403, 688
Eco31I GGTCTC 1 cut(s) 246
Eco53kI GAGCTC 1 cut(s) 686
Eco57I CTGAAG 1 cut(s) 909
EcoICRI GAGCTC 1 cut(s) 686
EcoRI GAATTC 1 cut(s) 292
EcoRII CCWGG 4 cut(s) 287, 332, 533, 937
EcoT14I CCWWGG 1 cut(s) 771
EcoT38I GRGCYC 2 cut(s) 403, 688
ErhI CCWWGG 1 cut(s) 771
FaeI CATG 4 cut(s) 149, 407, 469, 572
FalI AAGNNNNNCTT 2 cut(s) 93, 125
FaqI GGGAC 1 cut(s) 727
FatI CATG 4 cut(s) 145, 403, 465, 568
FauNDI CATATG 1 cut(s) 994
Fnu4HI GCNGC 1 cut(s) 285
FokI GGATG 5 cut(s) 404, 420, 703, 790, 921
FriOI GRGCYC 2 cut(s) 403, 688
Fsp4HI GCNGC 1 cut(s) 285
FspBI CTAG 3 cut(s) 51, 69, 590
GluI GCNGC 1 cut(s) 285
GsuI CTGGAG 2 cut(s) 562, 672
HaeIII GGCC 1 cut(s) 518
HapII CCGG 1 cut(s) 948
Hin1II CATG 4 cut(s) 149, 407, 469, 572
HincII GTYRAC 1 cut(s) 789
HindII GTYRAC 1 cut(s) 789
HinfI GANTC 2 cut(s) 4, 884
HpaII CCGG 1 cut(s) 948
HphI GGTGA 6 cut(s) 65, 121, 226, 233, 299, 439
Hpy166II GTNNAC 3 cut(s) 234, 789, 953
Hpy188I TCNGA 3 cut(s) 799, 817, 889
Hpy188III TCNNGA 6 cut(s) 146, 466, 579, 681, 909, 986
Hpy8I GTNNAC 3 cut(s) 234, 789, 953
HpyAV CCTTC 2 cut(s) 314, 431
HpyCH4III ACNGT 1 cut(s) 983
HpyCH4V TGCA 1 cut(s) 443
HpyF10VI GCNNNNNNNGC 1 cut(s) 857
HpyF3I CTNAG 6 cut(s) 165, 386, 675, 738, 847, 866
Hsp92II CATG 4 cut(s) 149, 407, 469, 572
Kzo9I GATC 6 cut(s) 157, 168, 223, 537, 862, 911
LmnI GCTCC 2 cut(s) 534, 691
Lsp1109I GCAGC 1 cut(s) 296
LweI GCATC 2 cut(s) 398, 725
MaeI CTAG 3 cut(s) 51, 69, 590
MaeIII GTNAC 5 cut(s) 71, 127, 445, 691, 750
MalI GATC 6 cut(s) 159, 170, 225, 539, 864, 913
MboI GATC 6 cut(s) 157, 168, 223, 537, 862, 911
MflI RGATCY 3 cut(s) 157, 168, 537
MhlI GDGCHC 2 cut(s) 403, 688
MlsI TGGCCA 1 cut(s) 518
MluNI TGGCCA 1 cut(s) 518
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 185
MnlI CCTC 7 cut(s) 57, 237, 390, 755, 769, 817, 925
Mox20I TGGCCA 1 cut(s) 518
MroXI GAANNNNTTC 1 cut(s) 648
MscI TGGCCA 1 cut(s) 518
MseI TTAA 6 cut(s) 33, 89, 135, 264, 341, 639
MslI CAYNNNNRTG 1 cut(s) 698
Msp20I TGGCCA 1 cut(s) 518
MspI CCGG 1 cut(s) 948
MspR9I CCNGG 5 cut(s) 289, 334, 535, 939, 948
MvaI CCWGG 4 cut(s) 289, 334, 535, 939
MwoI GCNNNNNNNGC 1 cut(s) 857
NciI CCSGG 1 cut(s) 948
NdeI CATATG 1 cut(s) 994
NdeII GATC 6 cut(s) 157, 168, 223, 537, 862, 911
NlaIII CATG 4 cut(s) 149, 407, 469, 572
NlaIV GGNNCC 2 cut(s) 159, 383
NmuCI GTSAC 5 cut(s) 71, 127, 445, 691, 750
NspI RCATGY 1 cut(s) 572
PagI TCATGA 2 cut(s) 145, 465
PciI ACATGT 1 cut(s) 568
PdmI GAANNNNTTC 1 cut(s) 648
PfeI GAWTC 1 cut(s) 884
PkrI GCNGC 1 cut(s) 286
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PscI ACATGT 1 cut(s) 568
PsiI TTATAA 1 cut(s) 99
Psp124BI GAGCTC 1 cut(s) 688
Psp6I CCWGG 4 cut(s) 287, 332, 533, 937
PspGI CCWGG 4 cut(s) 287, 332, 533, 937
PspN4I GGNNCC 2 cut(s) 159, 383
PsuI RGATCY 3 cut(s) 157, 168, 537
RseI CAYNNNNRTG 1 cut(s) 698
SacI GAGCTC 1 cut(s) 688
SaqAI TTAA 6 cut(s) 33, 89, 135, 264, 341, 639
SatI GCNGC 1 cut(s) 285
Sau3AI GATC 6 cut(s) 157, 168, 223, 537, 862, 911
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 5 cut(s) 289, 334, 535, 939, 948
SduI GDGCHC 2 cut(s) 403, 688
SetI ASST 8 cut(s) 70, 258, 452, 531, 673, 688, 739, 862
SfaNI GCATC 2 cut(s) 398, 725
SmiMI CAYNNNNRTG 1 cut(s) 698
SmlI CTYRAG 1 cut(s) 746
SmoI CTYRAG 1 cut(s) 746
SsiI CCGC 1 cut(s) 249
SspI AATATT 1 cut(s) 357
SspMI CTAG 3 cut(s) 51, 69, 590
SstI GAGCTC 1 cut(s) 688
StyD4I CCNGG 5 cut(s) 287, 332, 533, 937, 946
StyI CCWWGG 1 cut(s) 771
TaaI ACNGT 1 cut(s) 983
TfiI GAWTC 1 cut(s) 884
Tru1I TTAA 6 cut(s) 33, 89, 135, 264, 341, 639
Tru9I TTAA 6 cut(s) 33, 89, 135, 264, 341, 639
TseFI GTSAC 5 cut(s) 71, 127, 445, 691, 750
TseI GCWGC 1 cut(s) 284
Tsp45I GTSAC 5 cut(s) 71, 127, 445, 691, 750
TspDTI ATGAA 7 cut(s) 134, 294, 454, 482, 657, 897, 1011
TspGWI ACGGA 3 cut(s) 16, 185, 219
XapI RAATTY 8 cut(s) 92, 292, 343, 469, 490, 608, 647, 900
XceI RCATGY 1 cut(s) 572
XmnI GAANNNNTTC 1 cut(s) 648
XspI CTAG 3 cut(s) 51, 69, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.