Rmu_sc0004080.1_g000012

Villin-4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004080.1
OG0018355
Physical Location & Seq
Forward (+)
48644 .. 49285
642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004080.1_g000012.1.cds

Sequence Viewer

Length: 642 bp
atgtggttccttggatggtggagggctcatggttgccccttcgatatccttccacaagaagtaaaaaatgtagtcacctgtttctcgacagtcatgaacttcacaaattatgacttgaattttccaatcgcattacattttatggccaaatacaagctcccctggatcttcaaatggcattatgaagtcaaagaacatgtagtctccagacacttcctggtcaaatggtgggataaattcaaaactgaaagaattatctcccaatttaatgaagaatttccagtcactccatcacaacctactcagtcaagagctccagtcactccaatgccatcccaaacaacatccacttcccagtcacaacctaagtccctcaaatcacattcctcttccaaatccaaggggaaagcaaagtcaacaaaatcgtatacttcctccaagtcagaagaattgaaagagttagcaaaaagactcctagcacaagcagctaatttcagtaatgatgatgatgaatctgaagaagaagaagaaatttcttctcgatcctcatcctcatctcgcagtcatccaggcattcccagtaaaccggtcaaatgggctgattttcaagacagccaagacccatatgaagatttcgagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0006810 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010053 GO:0010054 GO:0010639 GO:0015629 GO:0016043 GO:0016049 GO:0016482 GO:0021700 GO:0022411 GO:0022622 GO:0030029 GO:0030036 GO:0030042 GO:0030154 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031333 GO:0032271 GO:0032272 GO:0032432 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0033043 GO:0033993 GO:0040007 GO:0042221 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043254 GO:0043624 GO:0043933 GO:0044087 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046907 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048523 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051014 GO:0051015 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051261 GO:0051493 GO:0051494 GO:0051592 GO:0051641 GO:0051649 GO:0051693 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0080147 GO:0090066 GO:0090558 GO:0090627 GO:0097305 GO:0097435 GO:0099402 GO:0099636 GO:0110053 GO:1901700 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04666 ko04810 ko05203 map04666 map04810 map05203
Pfam Domains
Protein Families
Gelsolin VHP

Protein Analysis

213

Amino Acids

24.68

Weight (kDa)

7.06

Isoelectric Point (pI)

62.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 428
AclWI GGATC 2 cut(s) 173, 537
AcoI YGGCCR 1 cut(s) 144
AcsI RAATTY 4 cut(s) 118, 236, 275, 531
AcuI CTGAAG 1 cut(s) 537
AflIII ACRYGT 1 cut(s) 196
AgeI ACCGGT 1 cut(s) 586
AgsI TTSAA 5 cut(s) 118, 172, 241, 454, 608
AjnI CCWGG 3 cut(s) 161, 216, 568
AluBI AGCT 3 cut(s) 157, 314, 488
AluI AGCT 3 cut(s) 157, 314, 488
Alw21I GWGCWC 1 cut(s) 316
Alw26I GTCTC 1 cut(s) 208
AlwI GGATC 2 cut(s) 173, 537
AoxI GGCC 1 cut(s) 144
ApeKI GCWGC 1 cut(s) 485
ApoI RAATTY 4 cut(s) 118, 236, 275, 531
AsiGI ACCGGT 1 cut(s) 586
Asp700I GAANNNNTTC 1 cut(s) 276
AsuHPI GGTGA 1 cut(s) 67
BalI TGGCCA 1 cut(s) 146
BanII GRGCYC 2 cut(s) 28, 316
Bbv12I GWGCWC 1 cut(s) 316
BbvI GCAGC 1 cut(s) 497
BccI CCATC 3 cut(s) 9, 298, 340
BciT130I CCWGG 3 cut(s) 163, 218, 570
BcoDI GTCTC 1 cut(s) 208
BfaI CTAG 1 cut(s) 476
BisI GCNGC 1 cut(s) 486
BlsI GCNGC 1 cut(s) 487
Bme1390I CCNGG 3 cut(s) 163, 218, 570
BmiI GGNNCC 1 cut(s) 8
BmrFI CCNGG 3 cut(s) 163, 218, 570
BmrI ACTGGG 2 cut(s) 349, 573
BmuI ACTGGG 2 cut(s) 349, 573
BpmI CTGGAG 2 cut(s) 190, 300
BsaBI GATNNNNATC 1 cut(s) 547
BsaJI CCNNGG 3 cut(s) 10, 161, 399
BsaWI WCCGGW 1 cut(s) 586
Bse118I RCCGGY 1 cut(s) 586
Bse1I ACTGG 4 cut(s) 281, 317, 355, 579
Bse8I GATNNNNATC 1 cut(s) 547
BseBI CCWGG 3 cut(s) 163, 218, 570
BseDI CCNNGG 3 cut(s) 10, 161, 399
BseGI GGATG 5 cut(s) 20, 332, 344, 548, 565
BseJI GATNNNNATC 1 cut(s) 547
BseMII CTCAG 1 cut(s) 317
BseNI ACTGG 4 cut(s) 281, 317, 355, 579
BseXI GCAGC 1 cut(s) 497
BshFI GGCC 1 cut(s) 146
BshTI ACCGGT 1 cut(s) 586
BsiHKAI GWGCWC 1 cut(s) 316
BsiSI CCGG 1 cut(s) 587
BslFI GGGAC 1 cut(s) 355
BsmAI GTCTC 1 cut(s) 208
BsmFI GGGAC 1 cut(s) 355
BsmI GAATGC 1 cut(s) 573
BsnI GGCC 1 cut(s) 146
Bsp1286I GDGCHC 2 cut(s) 28, 316
Bsp143I GATC 2 cut(s) 165, 542
BspANI GGCC 1 cut(s) 146
BspCNI CTCAG 1 cut(s) 316
BspHI TCATGA 1 cut(s) 93
BspLI GGNNCC 1 cut(s) 8
BspPI GGATC 2 cut(s) 173, 537
BsrFI RCCGGY 1 cut(s) 586
BsrI ACTGG 4 cut(s) 281, 317, 355, 579
BssAI RCCGGY 1 cut(s) 586
BssECI CCNNGG 3 cut(s) 10, 161, 399
BssMI GATC 2 cut(s) 165, 542
BssNAI GTATAC 1 cut(s) 429
BssT1I CCWWGG 2 cut(s) 10, 399
Bst1107I GTATAC 1 cut(s) 429
Bst2UI CCWGG 3 cut(s) 163, 218, 570
Bst4CI ACNGT 1 cut(s) 91
Bst6I CTCTTC 1 cut(s) 394
BstDEI CTNAG 2 cut(s) 303, 366
BstF5I GGATG 5 cut(s) 20, 332, 344, 548, 565
BstKTI GATC 2 cut(s) 168, 545
BstMAI GTCTC 1 cut(s) 208
BstMBI GATC 2 cut(s) 165, 542
BstMWI GCNNNNNNNGC 1 cut(s) 485
BstNI CCWGG 3 cut(s) 163, 218, 570
BstNSI RCATGY 1 cut(s) 200
BstSCI CCNGG 3 cut(s) 161, 216, 568
BstV1I GCAGC 1 cut(s) 497
BstX2I RGATCY 1 cut(s) 165
BstYI RGATCY 1 cut(s) 165
BstZ17I GTATAC 1 cut(s) 429
BsuRI GGCC 1 cut(s) 146
BtsCI GGATG 5 cut(s) 20, 332, 344, 548, 565
CciI TCATGA 1 cut(s) 93
Cfr10I RCCGGY 1 cut(s) 586
CspAI ACCGGT 1 cut(s) 586
CviAII CATG 3 cut(s) 29, 94, 197
CviJI RGCY 7 cut(s) 26, 146, 157, 314, 488, 599, 615
CviKI_1 RGCY 7 cut(s) 26, 146, 157, 314, 488, 599, 615
DdeI CTNAG 2 cut(s) 303, 366
DpnI GATC 2 cut(s) 167, 544
DpnII GATC 2 cut(s) 165, 542
EaeI YGGCCR 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 394
EarI CTCTTC 1 cut(s) 394
Ecl136II GAGCTC 1 cut(s) 314
Eco130I CCWWGG 2 cut(s) 10, 399
Eco24I GRGCYC 2 cut(s) 28, 316
Eco32I GATATC 1 cut(s) 46
Eco53kI GAGCTC 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 537
EcoICRI GAGCTC 1 cut(s) 314
EcoRII CCWGG 3 cut(s) 161, 216, 568
EcoRV GATATC 1 cut(s) 46
EcoT14I CCWWGG 2 cut(s) 10, 399
EcoT38I GRGCYC 2 cut(s) 28, 316
ErhI CCWWGG 2 cut(s) 10, 399
FaeI CATG 3 cut(s) 32, 97, 200
FaiI YATR 9 cut(s) 30, 95, 111, 143, 183, 198, 429, 625, 627
FaqI GGGAC 1 cut(s) 355
FatI CATG 3 cut(s) 28, 93, 196
FauNDI CATATG 1 cut(s) 625
FblI GTMKAC 1 cut(s) 428
Fnu4HI GCNGC 1 cut(s) 486
FokI GGATG 5 cut(s) 27, 319, 331, 535, 552
FriOI GRGCYC 2 cut(s) 28, 316
Fsp4HI GCNGC 1 cut(s) 486
FspBI CTAG 1 cut(s) 476
GluI GCNGC 1 cut(s) 486
GsuI CTGGAG 2 cut(s) 190, 300
HaeIII GGCC 1 cut(s) 146
HapII CCGG 1 cut(s) 587
Hin1II CATG 3 cut(s) 32, 97, 200
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HinfI GANTC 2 cut(s) 471, 512
HpaII CCGG 1 cut(s) 587
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 3 cut(s) 417, 429, 584
Hpy188I TCNGA 2 cut(s) 445, 517
Hpy188III TCNNGA 6 cut(s) 85, 94, 207, 309, 540, 608
Hpy8I GTNNAC 3 cut(s) 417, 429, 584
HpyAV CCTTC 2 cut(s) 49, 59
HpyCH4III ACNGT 1 cut(s) 91
HpyF10VI GCNNNNNNNGC 1 cut(s) 485
HpyF3I CTNAG 2 cut(s) 303, 366
Hsp92II CATG 3 cut(s) 32, 97, 200
Kzo9I GATC 2 cut(s) 165, 542
LmnI GCTCC 2 cut(s) 162, 319
Lsp1109I GCAGC 1 cut(s) 497
MaeI CTAG 1 cut(s) 476
MaeIII GTNAC 4 cut(s) 73, 283, 319, 357
MalI GATC 2 cut(s) 167, 544
MboI GATC 2 cut(s) 165, 542
MflI RGATCY 1 cut(s) 165
MhlI GDGCHC 2 cut(s) 28, 316
MlsI TGGCCA 1 cut(s) 146
MluCI AATT 9 cut(s) 106, 118, 236, 252, 263, 275, 449, 490, 531
MluNI TGGCCA 1 cut(s) 146
MlyI GAGTC 1 cut(s) 465
MnlI CCTC 6 cut(s) 15, 383, 397, 445, 556, 562
Mox20I TGGCCA 1 cut(s) 146
MroXI GAANNNNTTC 1 cut(s) 276
MscI TGGCCA 1 cut(s) 146
MseI TTAA 1 cut(s) 267
MslI CAYNNNNRTG 1 cut(s) 326
Msp20I TGGCCA 1 cut(s) 146
MspI CCGG 1 cut(s) 587
MspR9I CCNGG 3 cut(s) 163, 218, 570
Mva1269I GAATGC 1 cut(s) 573
MvaI CCWGG 3 cut(s) 163, 218, 570
MwoI GCNNNNNNNGC 1 cut(s) 485
NdeI CATATG 1 cut(s) 625
NdeII GATC 2 cut(s) 165, 542
NlaIII CATG 3 cut(s) 32, 97, 200
NlaIV GGNNCC 1 cut(s) 8
NmuCI GTSAC 4 cut(s) 73, 283, 319, 357
NspI RCATGY 1 cut(s) 200
PagI TCATGA 1 cut(s) 93
PciI ACATGT 1 cut(s) 196
PctI GAATGC 1 cut(s) 573
PdmI GAANNNNTTC 1 cut(s) 276
PfeI GAWTC 1 cut(s) 512
PinAI ACCGGT 1 cut(s) 586
PkrI GCNGC 1 cut(s) 487
PleI GAGTC 1 cut(s) 465
PpsI GAGTC 1 cut(s) 465
PscI ACATGT 1 cut(s) 196
Psp124BI GAGCTC 1 cut(s) 316
Psp6I CCWGG 3 cut(s) 161, 216, 568
PspGI CCWGG 3 cut(s) 161, 216, 568
PspN4I GGNNCC 1 cut(s) 8
PsuI RGATCY 1 cut(s) 165
RseI CAYNNNNRTG 1 cut(s) 326
SacI GAGCTC 1 cut(s) 316
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 1 cut(s) 486
Sau3AI GATC 2 cut(s) 165, 542
SchI GAGTC 1 cut(s) 465
ScrFI CCNGG 3 cut(s) 163, 218, 570
SduI GDGCHC 2 cut(s) 28, 316
SetI ASST 6 cut(s) 80, 159, 301, 316, 367, 490
SmiMI CAYNNNNRTG 1 cut(s) 326
Sse9I AATT 9 cut(s) 106, 118, 236, 252, 263, 275, 449, 490, 531
SspMI CTAG 1 cut(s) 476
SstI GAGCTC 1 cut(s) 316
StyD4I CCNGG 3 cut(s) 161, 216, 568
StyI CCWWGG 2 cut(s) 10, 399
TaaI ACNGT 1 cut(s) 91
TaqI TCGA 4 cut(s) 42, 86, 541, 636
TasI AATT 9 cut(s) 106, 118, 236, 252, 263, 275, 449, 490, 531
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TseFI GTSAC 4 cut(s) 73, 283, 319, 357
TseI GCWGC 1 cut(s) 485
Tsp45I GTSAC 4 cut(s) 73, 283, 319, 357
TspDTI ATGAA 5 cut(s) 110, 198, 285, 525, 642
XapI RAATTY 4 cut(s) 118, 236, 275, 531
XceI RCATGY 1 cut(s) 200
XcmI CCANNNNNNNNNTGG 1 cut(s) 214
XmiI GTMKAC 1 cut(s) 428
XmnI GAANNNNTTC 1 cut(s) 276
XspI CTAG 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.