Rmu_sc0007727.1_g000020

Villin-4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007727.1
OG0018355
Physical Location & Seq
Reverse (-)
83341 .. 85315
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007727.1_g000020.1.cds

Sequence Viewer

Length: 912 bp
atgagcccgacgaccaccgcatctcctccaccttccaaatccacacccaccaaacttaccccgcatctcatccacctcaccgctctaaaattcggagagatcttgcttcagcggcgagagattgggaaggcagttggcgaggtggtggtcgaggtcttgggcggcttggtggcggagcaagttgggttcggttcggggaggaccggaagaggcgatgagttggtggacctgggcgagtgggttttggaaaaaagaggaggggatggcagaggattaagtggagggaatgatgacggtggtgtgggagtggcgggagaggtggtggttgcaggtgagagagagagagagaaagagatgggtgaccagagtaagggagagatagagagatgggtgaccgatcacactacattttatggccaatacaagctcccctggatcttcaaatggcattacgaagtcaaagaacatgtagtctcaaggcatttcctagtcaaatggtgggataaattcaaaactgaaagaattatctcccaatttaatgaagaatttccagttactccatcacaacctactcagtcaagagctccagtcactccaatgccacctcaaacgacatccacttcccagtcacaacctacatccctcaagtcaccttcctcttccaagtcaaaggggaaagcaaaatcagcaaaatcaaatacatcctccaagtcagaagaattgaaagagttagcaaaaaagctcttagcacaagcagccactctcagtaatgataaagatgaatccgatgatgaagaagaaatttcttcccattcctcatcctcatcttgcagtcatccaggcactctcggtaaactagtcaaatgggctgatttccaagacagccaagacccatatgaagactatgagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0006810 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010053 GO:0010054 GO:0010639 GO:0015629 GO:0016043 GO:0016049 GO:0016482 GO:0021700 GO:0022411 GO:0022622 GO:0030029 GO:0030036 GO:0030042 GO:0030154 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031333 GO:0032271 GO:0032272 GO:0032432 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0033043 GO:0033993 GO:0040007 GO:0042221 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043254 GO:0043624 GO:0043933 GO:0044087 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046907 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048523 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051014 GO:0051015 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051261 GO:0051493 GO:0051494 GO:0051592 GO:0051641 GO:0051649 GO:0051693 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0080147 GO:0090066 GO:0090558 GO:0090627 GO:0097305 GO:0097435 GO:0099402 GO:0099636 GO:0110053 GO:1901700 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04666 ko04810 ko05203 map04666 map04810 map05203
Pfam Domains
Protein Families
Gelsolin VHP

Protein Analysis

303

Amino Acids

33.25

Weight (kDa)

5.72

Isoelectric Point (pI)

51.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 320
Acc36I ACCTGC 1 cut(s) 320
AccBSI CCGCTC 1 cut(s) 83
AciI CCGC 7 cut(s) 18, 62, 81, 112, 162, 173, 311
AclWI GGATC 1 cut(s) 443
AcoI YGGCCR 1 cut(s) 415
AcsI RAATTY 4 cut(s) 89, 506, 545, 801
AcuI CTGAAG 1 cut(s) 92
AfiI CCNNNNNNNGG 1 cut(s) 370
AflIII ACRYGT 1 cut(s) 466
AgsI TTSAA 3 cut(s) 442, 511, 724
AhlI ACTAGT 1 cut(s) 856
AjnI CCWGG 3 cut(s) 228, 431, 838
AluBI AGCT 3 cut(s) 427, 584, 742
AluI AGCT 3 cut(s) 427, 584, 742
Alw21I GWGCWC 1 cut(s) 586
Alw26I GTCTC 1 cut(s) 478
AlwI GGATC 1 cut(s) 443
AoxI GGCC 1 cut(s) 415
ApeKI GCWGC 1 cut(s) 755
ApoI RAATTY 4 cut(s) 89, 506, 545, 801
Asp700I GAANNNNTTC 1 cut(s) 546
AspS9I GGNCC 2 cut(s) 201, 226
AsuHPI GGTGA 5 cut(s) 70, 344, 371, 403, 642
AvaII GGWCC 2 cut(s) 201, 226
BalI TGGCCA 1 cut(s) 417
BanII GRGCYC 2 cut(s) 8, 586
BbsI GAAGAC 1 cut(s) 906
Bbv12I GWGCWC 1 cut(s) 586
BbvI GCAGC 1 cut(s) 767
BccI CCATC 4 cut(s) 257, 349, 381, 568
BciT130I CCWGG 3 cut(s) 230, 433, 840
BcoDI GTCTC 1 cut(s) 478
BcuI ACTAGT 1 cut(s) 856
BfaI CTAG 2 cut(s) 488, 857
BfuAI ACCTGC 1 cut(s) 320
BglII AGATCT 1 cut(s) 99
BisI GCNGC 3 cut(s) 113, 163, 756
BlsI GCNGC 3 cut(s) 114, 164, 757
Bme1390I CCNGG 3 cut(s) 230, 433, 840
Bme18I GGWCC 2 cut(s) 201, 226
BmgT120I GGNCC 2 cut(s) 201, 226
BmrFI CCNGG 3 cut(s) 230, 433, 840
BmrI ACTGGG 1 cut(s) 619
BmsI GCATC 2 cut(s) 29, 73
BmuI ACTGGG 1 cut(s) 619
BpiI GAAGAC 1 cut(s) 906
BpmI CTGGAG 1 cut(s) 570
BpuEI CTTGAG 2 cut(s) 460, 629
BsaJI CCNNGG 2 cut(s) 229, 431
BsaWI WCCGGW 1 cut(s) 203
BsaXI ACNNNNNCTCC 4 cut(s) 7, 37, 306, 336
Bsc4I CCNNNNNNNGG 1 cut(s) 370
Bse1I ACTGG 3 cut(s) 551, 587, 625
BseBI CCWGG 3 cut(s) 230, 433, 840
BseDI CCNNGG 2 cut(s) 229, 431
BseGI GGATG 7 cut(s) 69, 268, 614, 638, 701, 818, 835
BseLI CCNNNNNNNGG 1 cut(s) 370
BseMII CTCAG 2 cut(s) 587, 778
BseNI ACTGG 3 cut(s) 551, 587, 625
BseRI GAGGAG 2 cut(s) 15, 270
BseXI GCAGC 1 cut(s) 767
BshFI GGCC 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 586
BsiSI CCGG 1 cut(s) 204
BslI CCNNNNNNNGG 1 cut(s) 370
BsmAI GTCTC 1 cut(s) 478
BsnI GGCC 1 cut(s) 417
Bsp1286I GDGCHC 2 cut(s) 8, 586
Bsp143I GATC 3 cut(s) 99, 397, 435
BspACI CCGC 7 cut(s) 18, 62, 81, 112, 162, 173, 311
BspANI GGCC 1 cut(s) 417
BspCNI CTCAG 2 cut(s) 586, 777
BspMI ACCTGC 1 cut(s) 320
BspPI GGATC 1 cut(s) 443
BsrBI CCGCTC 1 cut(s) 83
BsrI ACTGG 3 cut(s) 551, 587, 625
BssECI CCNNGG 2 cut(s) 229, 431
BssMI GATC 3 cut(s) 99, 397, 435
Bst2UI CCWGG 3 cut(s) 230, 433, 840
Bst4CI ACNGT 1 cut(s) 296
Bst6I CTCTTC 2 cut(s) 202, 664
BstDEI CTNAG 3 cut(s) 573, 745, 764
BstEII GGTNACC 2 cut(s) 359, 391
BstF5I GGATG 7 cut(s) 69, 268, 614, 638, 701, 818, 835
BstKTI GATC 3 cut(s) 102, 400, 438
BstMAI GTCTC 1 cut(s) 478
BstMBI GATC 3 cut(s) 99, 397, 435
BstMWI GCNNNNNNNGC 3 cut(s) 112, 686, 755
BstNI CCWGG 3 cut(s) 230, 433, 840
BstNSI RCATGY 1 cut(s) 470
BstPI GGTNACC 2 cut(s) 359, 391
BstSCI CCNGG 3 cut(s) 228, 431, 838
BstV1I GCAGC 1 cut(s) 767
BstV2I GAAGAC 1 cut(s) 906
BstX2I RGATCY 2 cut(s) 99, 435
BstYI RGATCY 2 cut(s) 99, 435
BsuRI GGCC 1 cut(s) 417
BtgZI GCGATG 1 cut(s) 228
BtsCI GGATG 7 cut(s) 69, 268, 614, 638, 701, 818, 835
BveI ACCTGC 1 cut(s) 320
Cfr13I GGNCC 2 cut(s) 201, 226
CviAII CATG 1 cut(s) 467
CviJI RGCY 9 cut(s) 6, 165, 417, 427, 584, 742, 758, 869, 885
CviKI_1 RGCY 9 cut(s) 6, 165, 417, 427, 584, 742, 758, 869, 885
DdeI CTNAG 3 cut(s) 573, 745, 764
DpnI GATC 3 cut(s) 101, 399, 437
DpnII GATC 3 cut(s) 99, 397, 435
EaeI YGGCCR 1 cut(s) 415
Eam1104I CTCTTC 2 cut(s) 202, 664
EarI CTCTTC 2 cut(s) 202, 664
EciI GGCGGA 1 cut(s) 188
Ecl136II GAGCTC 1 cut(s) 584
Eco24I GRGCYC 2 cut(s) 8, 586
Eco47I GGWCC 2 cut(s) 201, 226
Eco53kI GAGCTC 1 cut(s) 584
Eco57I CTGAAG 1 cut(s) 92
Eco91I GGTNACC 2 cut(s) 359, 391
EcoICRI GAGCTC 1 cut(s) 584
EcoO65I GGTNACC 2 cut(s) 359, 391
EcoRII CCWGG 3 cut(s) 228, 431, 838
EcoT38I GRGCYC 2 cut(s) 8, 586
FaeI CATG 1 cut(s) 470
FaiI YATR 5 cut(s) 414, 468, 895, 897, 906
FatI CATG 1 cut(s) 466
FauI CCCGC 2 cut(s) 69, 304
FauNDI CATATG 1 cut(s) 895
Fnu4HI GCNGC 3 cut(s) 113, 163, 756
FokI GGATG 7 cut(s) 56, 275, 601, 625, 688, 805, 822
FriOI GRGCYC 2 cut(s) 8, 586
Fsp4HI GCNGC 3 cut(s) 113, 163, 756
FspBI CTAG 2 cut(s) 488, 857
GluI GCNGC 3 cut(s) 113, 163, 756
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 1 cut(s) 417
HapII CCGG 1 cut(s) 204
Hin1II CATG 1 cut(s) 470
HinfI GANTC 1 cut(s) 782
HpaII CCGG 1 cut(s) 204
HphI GGTGA 5 cut(s) 70, 344, 371, 403, 642
Hpy166II GTNNAC 2 cut(s) 226, 854
Hpy188I TCNGA 3 cut(s) 95, 715, 787
Hpy188III TCNNGA 1 cut(s) 579
Hpy8I GTNNAC 2 cut(s) 226, 854
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 3 cut(s) 42, 121, 663
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4V TGCA 2 cut(s) 329, 831
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 686, 755
HpyF3I CTNAG 3 cut(s) 573, 745, 764
Hsp92II CATG 1 cut(s) 470
Kzo9I GATC 3 cut(s) 99, 397, 435
LmnI GCTCC 3 cut(s) 175, 432, 589
Lsp1109I GCAGC 1 cut(s) 767
LweI GCATC 2 cut(s) 29, 73
MaeI CTAG 2 cut(s) 488, 857
MaeIII GTNAC 6 cut(s) 359, 391, 553, 589, 627, 648
MalI GATC 3 cut(s) 101, 399, 437
MbiI CCGCTC 1 cut(s) 83
MboI GATC 3 cut(s) 99, 397, 435
MboII GAAGA 9 cut(s) 219, 430, 554, 651, 728, 798, 806, 809, 911
MflI RGATCY 2 cut(s) 99, 435
MhlI GDGCHC 2 cut(s) 8, 586
MlsI TGGCCA 1 cut(s) 417
MluCI AATT 7 cut(s) 89, 506, 522, 533, 545, 719, 801
MluNI TGGCCA 1 cut(s) 417
Mox20I TGGCCA 1 cut(s) 417
MroXI GAANNNNTTC 1 cut(s) 546
MscI TGGCCA 1 cut(s) 417
MseI TTAA 2 cut(s) 275, 537
MslI CAYNNNNRTG 1 cut(s) 596
Msp20I TGGCCA 1 cut(s) 417
MspA1I CMGCKG 1 cut(s) 112
MspI CCGG 1 cut(s) 204
MspR9I CCNGG 3 cut(s) 230, 433, 840
MvaI CCWGG 3 cut(s) 230, 433, 840
MwoI GCNNNNNNNGC 3 cut(s) 112, 686, 755
NdeI CATATG 1 cut(s) 895
NdeII GATC 3 cut(s) 99, 397, 435
NlaIII CATG 1 cut(s) 470
NmuCI GTSAC 5 cut(s) 359, 391, 589, 627, 648
NspI RCATGY 1 cut(s) 470
PaqCI CACCTGC 1 cut(s) 320
PciI ACATGT 1 cut(s) 466
PdmI GAANNNNTTC 1 cut(s) 546
PfeI GAWTC 1 cut(s) 782
PkrI GCNGC 3 cut(s) 114, 164, 757
PscI ACATGT 1 cut(s) 466
Psp124BI GAGCTC 1 cut(s) 586
Psp6I CCWGG 3 cut(s) 228, 431, 838
PspEI GGTNACC 2 cut(s) 359, 391
PspGI CCWGG 3 cut(s) 228, 431, 838
PspPI GGNCC 2 cut(s) 201, 226
PsuI RGATCY 2 cut(s) 99, 435
RseI CAYNNNNRTG 1 cut(s) 596
SacI GAGCTC 1 cut(s) 586
SaqAI TTAA 2 cut(s) 275, 537
SatI GCNGC 3 cut(s) 113, 163, 756
Sau3AI GATC 3 cut(s) 99, 397, 435
Sau96I GGNCC 2 cut(s) 201, 226
ScrFI CCNGG 3 cut(s) 230, 433, 840
SduI GDGCHC 2 cut(s) 8, 586
SfaNI GCATC 2 cut(s) 29, 73
SinI GGWCC 2 cut(s) 201, 226
SmiMI CAYNNNNRTG 1 cut(s) 596
SmlI CTYRAG 2 cut(s) 475, 644
SmoI CTYRAG 2 cut(s) 475, 644
SpeI ACTAGT 1 cut(s) 856
Sse9I AATT 7 cut(s) 89, 506, 522, 533, 545, 719, 801
SsiI CCGC 7 cut(s) 18, 62, 81, 112, 162, 173, 311
SspMI CTAG 2 cut(s) 488, 857
SstI GAGCTC 1 cut(s) 586
StyD4I CCNGG 3 cut(s) 228, 431, 838
TaaI ACNGT 1 cut(s) 296
TaqI TCGA 1 cut(s) 150
TaqII GACCGA 1 cut(s) 410
TasI AATT 7 cut(s) 89, 506, 522, 533, 545, 719, 801
TauI GCSGC 2 cut(s) 115, 165
TfiI GAWTC 1 cut(s) 782
Tru1I TTAA 2 cut(s) 275, 537
Tru9I TTAA 2 cut(s) 275, 537
TseFI GTSAC 5 cut(s) 359, 391, 589, 627, 648
TseI GCWGC 1 cut(s) 755
Tsp45I GTSAC 5 cut(s) 359, 391, 589, 627, 648
TspDTI ATGAA 4 cut(s) 555, 795, 807, 912
VpaK11BI GGWCC 2 cut(s) 201, 226
XapI RAATTY 4 cut(s) 89, 506, 545, 801
XceI RCATGY 1 cut(s) 470
XmnI GAANNNNTTC 1 cut(s) 546
XspI CTAG 2 cut(s) 488, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.