FvH4_1g24140

tRNA (Guanine-1)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012259
Physical Location & Seq
Forward (+)
16014859 .. 16016439
1581 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g24140.t2

Sequence Viewer

Length: 1032 bp
ATGGAGGTCGTAGAAAATGGGGAGAAAGCCACGGAATGTCCAAACTGCTCGGCGGCGGCAGCAGCAGCAGCAGCCGGAGTACTATCGAAGAGTGCCCAGAAGAAGCTGCTAAAGCAGCAGAGGTGGGAGGCGAAGAAGGCGGAGAAGAAAGCGCAGGAGAAAGAGCACAAGAAGAGAGAAGGAGAGCGGAAGCGAAAGGAATGGGAGGAGACTCTTGCCAGCTTAACCGAAGAGGAAAGGTTGAAGATGATAGAGTCCAGGAAGAGCTTGAGAAAGGAGAGAATGGACAAGAGGTCGGAAGAGCGAGACAGAAAAAACGAGCGGCTCAACAGAGCCAAATTGTCTGGACAGAAGATGGTGATTGATCTTGAGTTCTCTCACCTCATGACTCCCTCGGAGATCAACAGCCTCGTTCACCAGATTATGTATTGTTATGCGGAGAACGGGAGGAGTAGTGAGCCATGTCATCTGTGGTTGACTGGGTGCAAGGGAGAGATGGGTACACAGCTGCGGAGGTTGCCCGGGTTTGATAACTGGAGTGTTGAGAGGGAGGATAGGTCCTACATTGAAGCTTTGGAGGGTGAGAAGGAAAACCTGGTGTATCTGACGGCTGATTCGGAGAATGTGGTGGATGAGCTGGATCGGAGCAAGATATATATTATTGGGGGATTGGTGGATCGGAATAGGTGGAAGGGGATCACAATGAAGAAAGCTGAGGAGCAGGGGATACAGACTGCGAAGCTCCCAATTGGGAAGTACTTGAACATGTGCAGTTCCCAGGTCCTGACTGTGAATCAAGTGGTTGAGATTCTCCTCAAGTACTTGGAAACCAAAGACTGGAAGGCTTCCTTCTTCCAAGTGATTCCGCAGCGAAAGCGATTGCAAGCAGCTGAGGATTCATCAGAGGAACCTCAACAAGAGGAAGTAGAAGGAAAGGAAAAAAGTGAAGAGAATGATGGTGATAATCAATATGAAAGCAAAAAGCAGTGTGCAAATGGCCCTCCTTCGATGGAGCAAGTGCCTGCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_m1G_MT

Protein Analysis

344

Amino Acids

39.38

Weight (kDa)

6.27

Isoelectric Point (pI)

56.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 837
AccBSI CCGCTC 2 cut(s) 187, 322
AciI CCGC 8 cut(s) 53, 56, 140, 187, 322, 437, 511, 866
AclWI GGATC 3 cut(s) 648, 684, 704
AfaI GTAC 4 cut(s) 81, 502, 758, 821
AfiI CCNNNNNNNGG 1 cut(s) 837
AflIII ACRYGT 1 cut(s) 765
AgsI TTSAA 3 cut(s) 244, 569, 763
AhdI GACNNNNNGTC 1 cut(s) 292
AjnI CCWGG 3 cut(s) 257, 594, 777
AluBI AGCT 9 cut(s) 106, 222, 267, 508, 572, 637, 713, 742, 890
AluI AGCT 9 cut(s) 106, 222, 267, 508, 572, 637, 713, 742, 890
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 2 cut(s) 203, 300
AlwI GGATC 3 cut(s) 648, 684, 704
AlwNI CAGNNNCTG 1 cut(s) 784
Ama87I CYCGRG 1 cut(s) 521
AoxI GGCC 1 cut(s) 997
ArsI GACNNNNNNTTYG 2 cut(s) 598, 630
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 3 cut(s) 558, 781, 998
AsuC2I CCSGG 2 cut(s) 522, 523
AsuHPI GGTGA 5 cut(s) 370, 371, 407, 593, 971
AvaI CYCGRG 1 cut(s) 521
AvaII GGWCC 2 cut(s) 558, 781
BaeGI GKGCMC 1 cut(s) 97
Bbv12I GWGCWC 1 cut(s) 168
BbvCI CCTCAGC 2 cut(s) 714, 891
BccI CCATC 4 cut(s) 349, 490, 950, 1003
BceAI ACGGC 1 cut(s) 624
BciT130I CCWGG 3 cut(s) 259, 596, 779
BciVI GTATCC 1 cut(s) 720
BcnI CCSGG 2 cut(s) 522, 523
BcoDI GTCTC 2 cut(s) 203, 300
BfuI GTATCC 1 cut(s) 720
BmcAI AGTACT 3 cut(s) 81, 758, 821
Bme1390I CCNGG 5 cut(s) 259, 522, 523, 596, 779
Bme18I GGWCC 2 cut(s) 558, 781
BmeRI GACNNNNNGTC 1 cut(s) 292
BmeT110I CYCGRG 1 cut(s) 521
BmgT120I GGNCC 3 cut(s) 558, 781, 998
BmiI GGNNCC 1 cut(s) 909
BmrFI CCNGG 5 cut(s) 259, 522, 523, 596, 779
BmrI ACTGGG 1 cut(s) 489
BmuI ACTGGG 1 cut(s) 489
BpmI CTGGAG 1 cut(s) 556
Bpu10I CCTNAGC 2 cut(s) 714, 891
BpuEI CTTGAG 3 cut(s) 289, 389, 800
BpuMI CCSGG 2 cut(s) 522, 523
BsaJI CCNNGG 4 cut(s) 30, 393, 521, 777
BsaXI ACNNNNNCTCC 4 cut(s) 439, 469, 542, 572
Bsc4I CCNNNNNNNGG 1 cut(s) 837
Bse1I ACTGG 3 cut(s) 484, 539, 842
BseBI CCWGG 3 cut(s) 259, 596, 779
BseDI CCNNGG 4 cut(s) 30, 393, 521, 777
BseGI GGATG 1 cut(s) 637
BseLI CCNNNNNNNGG 1 cut(s) 837
BseMII CTCAG 2 cut(s) 705, 882
BseNI ACTGG 3 cut(s) 484, 539, 842
BseRI GAGGAG 4 cut(s) 221, 463, 731, 803
BseSI GKGCMC 1 cut(s) 97
BsgI GTGCAG 1 cut(s) 790
BshFI GGCC 1 cut(s) 999
BsiHKAI GWGCWC 1 cut(s) 168
BsiHKCI CYCGRG 1 cut(s) 521
BsiSI CCGG 2 cut(s) 75, 522
BslI CCNNNNNNNGG 1 cut(s) 837
BsmAI GTCTC 2 cut(s) 203, 300
BsnI GGCC 1 cut(s) 999
BsoBI CYCGRG 1 cut(s) 521
Bsp1286I GDGCHC 2 cut(s) 97, 168
Bsp143I GATC 5 cut(s) 364, 399, 640, 676, 696
BspACI CCGC 8 cut(s) 53, 56, 140, 187, 322, 437, 511, 866
BspANI GGCC 1 cut(s) 999
BspCNI CTCAG 2 cut(s) 706, 883
BspHI TCATGA 1 cut(s) 384
BspLI GGNNCC 1 cut(s) 909
BspPI GGATC 3 cut(s) 648, 684, 704
BspQI GCTCTTC 2 cut(s) 257, 294
BsrBI CCGCTC 2 cut(s) 187, 322
BsrI ACTGG 3 cut(s) 484, 539, 842
BssECI CCNNGG 4 cut(s) 30, 393, 521, 777
BssMI GATC 5 cut(s) 364, 399, 640, 676, 696
Bst2UI CCWGG 3 cut(s) 259, 596, 779
Bst4CI ACNGT 1 cut(s) 790
Bst6I CTCTTC 6 cut(s) 83, 167, 225, 257, 294, 942
BstC8I GCNNGC 3 cut(s) 220, 885, 1023
BstDEI CTNAG 2 cut(s) 714, 891
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 1 cut(s) 637
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 5 cut(s) 367, 402, 643, 679, 699
BstMAI GTCTC 2 cut(s) 203, 300
BstMBI GATC 5 cut(s) 364, 399, 640, 676, 696
BstNI CCWGG 3 cut(s) 259, 596, 779
BstNSI RCATGY 1 cut(s) 769
BstSCI CCNGG 5 cut(s) 257, 520, 521, 594, 777
BstSLI GKGCMC 1 cut(s) 97
BsuI GTATCC 1 cut(s) 720
BsuRI GGCC 1 cut(s) 999
BtgI CCRYGG 1 cut(s) 30
BtsCI GGATG 1 cut(s) 637
BtsI GCAGTG 1 cut(s) 992
BtsIMutI CAGTG 1 cut(s) 992
Cac8I GCNNGC 3 cut(s) 220, 885, 1023
CaiI CAGNNNCTG 1 cut(s) 784
CciI TCATGA 1 cut(s) 384
CfoI GCGC 1 cut(s) 154
Cfr13I GGNCC 3 cut(s) 558, 781, 998
Cfr9I CCCGGG 1 cut(s) 521
CsiI ACCWGGT 1 cut(s) 594
Csp6I GTAC 4 cut(s) 80, 501, 757, 820
CviAII CATG 3 cut(s) 385, 462, 766
CviQI GTAC 4 cut(s) 80, 501, 757, 820
DdeI CTNAG 2 cut(s) 714, 891
DpnI GATC 5 cut(s) 366, 401, 642, 678, 698
DpnII GATC 5 cut(s) 364, 399, 640, 676, 696
DriI GACNNNNNGTC 1 cut(s) 292
Eam1104I CTCTTC 6 cut(s) 83, 167, 225, 257, 294, 942
Eam1105I GACNNNNNGTC 1 cut(s) 292
EarI CTCTTC 6 cut(s) 83, 167, 225, 257, 294, 942
EciI GGCGGA 1 cut(s) 155
Eco47I GGWCC 2 cut(s) 558, 781
Eco88I CYCGRG 1 cut(s) 521
EcoO109I RGGNCCY 2 cut(s) 558, 781
EcoRII CCWGG 3 cut(s) 257, 594, 777
FaeI CATG 3 cut(s) 388, 465, 769
FaiI YATR 8 cut(s) 386, 425, 435, 463, 655, 657, 767, 972
FalI AAGNNNNNCTT 2 cut(s) 833, 865
FatI CATG 3 cut(s) 384, 461, 765
FokI GGATG 1 cut(s) 644
GlaI GCGC 1 cut(s) 153
GsuI CTGGAG 1 cut(s) 556
HaeIII GGCC 1 cut(s) 999
HapII CCGG 2 cut(s) 75, 522
HhaI GCGC 1 cut(s) 154
Hin1II CATG 3 cut(s) 388, 465, 769
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HincII GTYRAC 1 cut(s) 477
HindII GTYRAC 1 cut(s) 477
HindIII AAGCTT 1 cut(s) 570
HinfI GANTC 8 cut(s) 211, 254, 388, 614, 793, 808, 862, 896
HpaII CCGG 2 cut(s) 75, 522
HphI GGTGA 5 cut(s) 370, 371, 407, 593, 971
Hpy166II GTNNAC 3 cut(s) 415, 477, 503
Hpy188I TCNGA 7 cut(s) 298, 397, 606, 619, 645, 681, 904
Hpy188III TCNNGA 4 cut(s) 345, 368, 385, 784
Hpy8I GTNNAC 3 cut(s) 415, 477, 503
HpyAV CCTTC 8 cut(s) 130, 173, 580, 685, 835, 859, 923, 1014
HpyCH4III ACNGT 1 cut(s) 790
HpyCH4V TGCA 4 cut(s) 486, 771, 883, 992
HpyF3I CTNAG 2 cut(s) 714, 891
Hsp92II CATG 3 cut(s) 388, 465, 769
HspAI GCGC 1 cut(s) 152
Kzo9I GATC 5 cut(s) 364, 399, 640, 676, 696
LguI GCTCTTC 2 cut(s) 257, 294
LmnI GCTCC 4 cut(s) 645, 718, 747, 1012
MabI ACCWGGT 1 cut(s) 594
MalI GATC 5 cut(s) 366, 401, 642, 678, 698
MbiI CCGCTC 2 cut(s) 187, 322
MboI GATC 5 cut(s) 364, 399, 640, 676, 696
MfeI CAATTG 1 cut(s) 747
MhlI GDGCHC 2 cut(s) 97, 168
MluCI AATT 2 cut(s) 338, 747
MlyI GAGTC 3 cut(s) 205, 263, 382
MmeI TCCRAC 1 cut(s) 276
MseI TTAA 1 cut(s) 224
MspA1I CMGCKG 2 cut(s) 508, 890
MspI CCGG 2 cut(s) 75, 522
MspR9I CCNGG 5 cut(s) 259, 522, 523, 596, 779
MunI CAATTG 1 cut(s) 747
MvaI CCWGG 3 cut(s) 259, 596, 779
NciI CCSGG 2 cut(s) 522, 523
NdeII GATC 5 cut(s) 364, 399, 640, 676, 696
NlaIII CATG 3 cut(s) 388, 465, 769
NlaIV GGNNCC 1 cut(s) 909
NmeAIII GCCGAG 1 cut(s) 29
NspI RCATGY 1 cut(s) 769
PagI TCATGA 1 cut(s) 384
PciI ACATGT 1 cut(s) 765
PciSI GCTCTTC 2 cut(s) 257, 294
PfeI GAWTC 5 cut(s) 614, 793, 808, 862, 896
PflMI CCANNNNNTGG 1 cut(s) 837
PfoI TCCNGGA 1 cut(s) 257
PleI GAGTC 3 cut(s) 205, 262, 382
PpsI GAGTC 3 cut(s) 205, 262, 382
PpuMI RGGWCCY 2 cut(s) 558, 781
PscI ACATGT 1 cut(s) 765
Psp5II RGGWCCY 2 cut(s) 558, 781
Psp6I CCWGG 3 cut(s) 257, 594, 777
PspGI CCWGG 3 cut(s) 257, 594, 777
PspN4I GGNNCC 1 cut(s) 909
PspPI GGNCC 3 cut(s) 558, 781, 998
PspPPI RGGWCCY 2 cut(s) 558, 781
PstNI CAGNNNCTG 1 cut(s) 784
PvuII CAGCTG 2 cut(s) 508, 890
RsaI GTAC 4 cut(s) 81, 502, 758, 821
RsaNI GTAC 4 cut(s) 80, 501, 757, 820
SapI GCTCTTC 2 cut(s) 257, 294
SaqAI TTAA 1 cut(s) 224
Sau3AI GATC 5 cut(s) 364, 399, 640, 676, 696
Sau96I GGNCC 3 cut(s) 558, 781, 998
ScaI AGTACT 3 cut(s) 81, 758, 821
SchI GAGTC 3 cut(s) 205, 263, 382
ScrFI CCNGG 5 cut(s) 259, 522, 523, 596, 779
SduI GDGCHC 2 cut(s) 97, 168
SexAI ACCWGGT 1 cut(s) 594
SinI GGWCC 2 cut(s) 558, 781
SmaI CCCGGG 1 cut(s) 523
SmlI CTYRAG 3 cut(s) 268, 368, 815
SmoI CTYRAG 3 cut(s) 268, 368, 815
Sse9I AATT 2 cut(s) 338, 747
SsiI CCGC 8 cut(s) 53, 56, 140, 187, 322, 437, 511, 866
StyD4I CCNGG 5 cut(s) 257, 520, 521, 594, 777
TaaI ACNGT 1 cut(s) 790
TaqI TCGA 2 cut(s) 86, 1007
TasI AATT 2 cut(s) 338, 747
TatI WGTACW 3 cut(s) 79, 756, 819
TauI GCSGC 3 cut(s) 56, 59, 325
TfiI GAWTC 5 cut(s) 614, 793, 808, 862, 896
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TscAI CASTG 1 cut(s) 992
TspDTI ATGAA 3 cut(s) 719, 888, 987
TspGWI ACGGA 1 cut(s) 47
TspMI CCCGGG 1 cut(s) 521
TspRI CASTG 1 cut(s) 992
Van91I CCANNNNNTGG 1 cut(s) 837
VpaK11BI GGWCC 2 cut(s) 558, 781
XceI RCATGY 1 cut(s) 769
XcmI CCANNNNNNNNNTGG 1 cut(s) 468
XmaI CCCGGG 1 cut(s) 521
ZrmI AGTACT 3 cut(s) 81, 758, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.