Rh2BG296200

tRNA (Guanine-1)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012259
Physical Location & Seq
Forward (+)
33816747 .. 33818513
1767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG296200.1

Sequence Viewer

Length: 804 bp
ATGGAGGGCGTAGAAAATGGGGGAAAAGCCACGCAATGTCCGAACTGCTCGGCAGCAGTAGTGCTATCGAAGAGTGCCCAGAAGAAGGTGTTAAAGCAGCAGAGGTGGGAGGCGAAGAAGGCGGAGAAGAAGGCCCAGGAAAAAGAGCACAAGAAGAGAGAGGGAGAGCGGAAGCGGAAGGAATGGGAGGAGACTCTGGCGAGCTTGAGCGAAGACGAGAGGTTGAAGATGATAGAGTCCAGGAAGAGCTTGAGGAAGGAGAGAATGGACAAGAGGTCAGAGGAGCGAGACCGAAAGACCGAGCGGCTCACCAGAGCCAAGGAGTGTGGACAGAAGATTGTGATTGATCTCGAGTTCTCTCACCTCATGACGCCCTCTGAGACCCACAGCCTCGTTCAACAGATTATGTATTGTTATGCGGTGAATGGGAGGAGTAGGGAGCCGTGTCATCTGTGGCTGACTGGGTGTAAAGGAGAGATGGGTACGCAGTTGCGCAGGCTGCCGGGGTTTGATAAGTGGAGTGTTGAGAGGGAAGATGGGTCCTACATTGAGGCTTTGGAGAGTGAGAAGAACAATCTGGTGTATCTGACGGCTGATTCGGAAAATGTGGTGGATGATCTTGATCGGAGCAAGATATATATTATTGGGGGATTGGTGGATCGGAATAGGTGGAAAGGAATCACAATGAAGAAAGCAGAGGACCAGGGAATCCAAACTGCGAAGCTCCCCATCGGCAAGTACTTGAACATGTGCAGTTCCCAGGTACTCACTCGGTTTCATGATGATGATCATTTTTCGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_m1G_MT

Protein Analysis

267

Amino Acids

30.93

Weight (kDa)

8.82

Isoelectric Point (pI)

47.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 494
AccBSI CCGCTC 2 cut(s) 169, 304
AciI CCGC 5 cut(s) 122, 169, 175, 304, 419
AclWI GGATC 1 cut(s) 666
AcyI GRCGYC 1 cut(s) 371
AfaI GTAC 3 cut(s) 484, 740, 765
AfiI CCNNNNNNNGG 1 cut(s) 85
AflIII ACRYGT 1 cut(s) 747
AgsI TTSAA 3 cut(s) 226, 398, 745
AhdI GACNNNNNGTC 1 cut(s) 274
AjnI CCWGG 4 cut(s) 135, 239, 702, 759
AluBI AGCT 3 cut(s) 204, 249, 724
AluI AGCT 3 cut(s) 204, 249, 724
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 3 cut(s) 185, 282, 374
AlwI GGATC 1 cut(s) 666
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 1 cut(s) 132
ApeKI GCWGC 3 cut(s) 53, 97, 499
ArsI GACNNNNNNTTYG 2 cut(s) 580, 612
AspLEI GCGC 1 cut(s) 495
AspS9I GGNCC 3 cut(s) 133, 540, 700
AsuC2I CCSGG 1 cut(s) 504
AsuHPI GGTGA 3 cut(s) 301, 353, 433
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 2 cut(s) 540, 700
BaeGI GKGCMC 1 cut(s) 79
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 3 cut(s) 65, 109, 486
BccI CCATC 3 cut(s) 472, 530, 737
BceAI ACGGC 2 cut(s) 427, 606
BciT130I CCWGG 4 cut(s) 137, 241, 704, 761
BclI TGATCA 1 cut(s) 787
BcnI CCSGG 1 cut(s) 504
BcoDI GTCTC 3 cut(s) 185, 282, 374
BisI GCNGC 4 cut(s) 54, 98, 305, 500
BlsI GCNGC 4 cut(s) 55, 99, 306, 501
BmcAI AGTACT 1 cut(s) 740
Bme1390I CCNGG 5 cut(s) 137, 241, 504, 704, 761
Bme18I GGWCC 2 cut(s) 540, 700
BmeRI GACNNNNNGTC 1 cut(s) 274
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 3 cut(s) 133, 540, 700
BmiI GGNNCC 2 cut(s) 441, 541
BmrFI CCNGG 5 cut(s) 137, 241, 504, 704, 761
BmrI ACTGGG 1 cut(s) 471
BmuI ACTGGG 1 cut(s) 471
BpiI GAAGAC 1 cut(s) 219
BpuEI CTTGAG 2 cut(s) 226, 271
BpuMI CCSGG 1 cut(s) 504
BsaBI GATNNNNATC 2 cut(s) 621, 786
BsaHI GRCGYC 1 cut(s) 371
BsaI GGTCTC 2 cut(s) 282, 374
BsaJI CCNNGG 5 cut(s) 135, 318, 503, 703, 759
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse1I ACTGG 1 cut(s) 466
Bse3DI GCAATG 1 cut(s) 41
Bse8I GATNNNNATC 2 cut(s) 621, 786
BseBI CCWGG 4 cut(s) 137, 241, 704, 761
BseDI CCNNGG 5 cut(s) 135, 318, 503, 703, 759
BseGI GGATG 1 cut(s) 619
BseJI GATNNNNATC 2 cut(s) 621, 786
BseLI CCNNNNNNNGG 1 cut(s) 85
BseMI GCAATG 1 cut(s) 41
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 1 cut(s) 466
BseRI GAGGAG 3 cut(s) 203, 296, 445
BseSI GKGCMC 1 cut(s) 79
BseXI GCAGC 3 cut(s) 65, 109, 486
BsgI GTGCAG 1 cut(s) 772
BshFI GGCC 1 cut(s) 134
BsiHKAI GWGCWC 1 cut(s) 150
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 1 cut(s) 503
BslI CCNNNNNNNGG 1 cut(s) 85
BsmAI GTCTC 3 cut(s) 185, 282, 374
BsnI GGCC 1 cut(s) 134
Bso31I GGTCTC 2 cut(s) 282, 374
BsoBI CYCGRG 1 cut(s) 350
Bsp1286I GDGCHC 2 cut(s) 79, 150
Bsp143I GATC 5 cut(s) 346, 616, 622, 658, 787
BspACI CCGC 5 cut(s) 122, 169, 175, 304, 419
BspANI GGCC 1 cut(s) 134
BspCNI CTCAG 1 cut(s) 370
BspHI TCATGA 2 cut(s) 366, 778
BspLI GGNNCC 2 cut(s) 441, 541
BspPI GGATC 1 cut(s) 666
BspQI GCTCTTC 1 cut(s) 239
BspTNI GGTCTC 2 cut(s) 282, 374
BsrBI CCGCTC 2 cut(s) 169, 304
BsrDI GCAATG 1 cut(s) 41
BsrI ACTGG 1 cut(s) 466
BssECI CCNNGG 5 cut(s) 135, 318, 503, 703, 759
BssMI GATC 5 cut(s) 346, 616, 622, 658, 787
BssNI GRCGYC 1 cut(s) 371
BssT1I CCWWGG 1 cut(s) 318
Bst2UI CCWGG 4 cut(s) 137, 241, 704, 761
Bst6I CTCTTC 3 cut(s) 65, 149, 239
BstACI GRCGYC 1 cut(s) 371
BstC8I GCNNGC 2 cut(s) 202, 497
BstDEI CTNAG 1 cut(s) 378
BstF5I GGATG 1 cut(s) 619
BstHHI GCGC 1 cut(s) 495
BstKTI GATC 5 cut(s) 349, 619, 625, 661, 790
BstMAI GTCTC 3 cut(s) 185, 282, 374
BstMBI GATC 5 cut(s) 346, 616, 622, 658, 787
BstMWI GCNNNNNNNGC 2 cut(s) 119, 499
BstNI CCWGG 4 cut(s) 137, 241, 704, 761
BstNSI RCATGY 1 cut(s) 751
BstSCI CCNGG 5 cut(s) 135, 239, 502, 702, 759
BstSLI GKGCMC 1 cut(s) 79
BstV1I GCAGC 3 cut(s) 65, 109, 486
BstV2I GAAGAC 1 cut(s) 219
BsuRI GGCC 1 cut(s) 134
BtsCI GGATG 1 cut(s) 619
Cac8I GCNNGC 2 cut(s) 202, 497
CciI TCATGA 2 cut(s) 366, 778
CfoI GCGC 1 cut(s) 495
Cfr13I GGNCC 3 cut(s) 133, 540, 700
CseI GACGC 1 cut(s) 379
Csp6I GTAC 3 cut(s) 483, 739, 764
CspCI CAANNNNNGTGG 2 cut(s) 307, 342
CviAII CATG 3 cut(s) 367, 748, 779
CviQI GTAC 3 cut(s) 483, 739, 764
DdeI CTNAG 1 cut(s) 378
DpnI GATC 5 cut(s) 348, 618, 624, 660, 789
DpnII GATC 5 cut(s) 346, 616, 622, 658, 787
DriI GACNNNNNGTC 1 cut(s) 274
Eam1104I CTCTTC 3 cut(s) 65, 149, 239
Eam1105I GACNNNNNGTC 1 cut(s) 274
EarI CTCTTC 3 cut(s) 65, 149, 239
EciI GGCGGA 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 318
Eco31I GGTCTC 2 cut(s) 282, 374
Eco47I GGWCC 2 cut(s) 540, 700
Eco88I CYCGRG 1 cut(s) 350
EcoO109I RGGNCCY 1 cut(s) 540
EcoRII CCWGG 4 cut(s) 135, 239, 702, 759
EcoT14I CCWWGG 1 cut(s) 318
ErhI CCWWGG 1 cut(s) 318
FaeI CATG 3 cut(s) 370, 751, 782
FaiI YATR 7 cut(s) 368, 407, 417, 637, 639, 749, 780
FatI CATG 3 cut(s) 366, 747, 778
FbaI TGATCA 1 cut(s) 787
Fnu4HI GCNGC 4 cut(s) 54, 98, 305, 500
FokI GGATG 1 cut(s) 626
Fsp4HI GCNGC 4 cut(s) 54, 98, 305, 500
FspI TGCGCA 1 cut(s) 494
GlaI GCGC 1 cut(s) 494
GluI GCNGC 4 cut(s) 54, 98, 305, 500
HaeIII GGCC 1 cut(s) 134
HapII CCGG 1 cut(s) 503
HgaI GACGC 1 cut(s) 379
HhaI GCGC 1 cut(s) 495
Hin1I GRCGYC 1 cut(s) 371
Hin1II CATG 3 cut(s) 370, 751, 782
Hin6I GCGC 1 cut(s) 493
HinP1I GCGC 1 cut(s) 493
HinfI GANTC 5 cut(s) 193, 236, 596, 678, 708
HpaII CCGG 1 cut(s) 503
HphI GGTGA 3 cut(s) 301, 353, 433
Hpy166II GTNNAC 1 cut(s) 329
Hpy188I TCNGA 8 cut(s) 42, 280, 379, 588, 601, 627, 663, 799
Hpy188III TCNNGA 4 cut(s) 350, 367, 620, 779
Hpy8I GTNNAC 1 cut(s) 329
HpyAV CCTTC 5 cut(s) 79, 112, 124, 172, 250
HpyCH4V TGCA 1 cut(s) 753
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 499
HpyF3I CTNAG 1 cut(s) 378
Hsp92I GRCGYC 1 cut(s) 371
Hsp92II CATG 3 cut(s) 370, 751, 782
HspAI GCGC 1 cut(s) 493
Ksp22I TGATCA 1 cut(s) 787
Kzo9I GATC 5 cut(s) 346, 616, 622, 658, 787
LguI GCTCTTC 1 cut(s) 239
LmnI GCTCC 4 cut(s) 283, 439, 627, 729
Lsp1109I GCAGC 3 cut(s) 65, 109, 486
MalI GATC 5 cut(s) 348, 618, 624, 660, 789
MbiI CCGCTC 2 cut(s) 169, 304
MboI GATC 5 cut(s) 346, 616, 622, 658, 787
MhlI GDGCHC 2 cut(s) 79, 150
MlyI GAGTC 2 cut(s) 187, 245
MseI TTAA 1 cut(s) 92
MslI CAYNNNNRTG 1 cut(s) 783
MspI CCGG 1 cut(s) 503
MspR9I CCNGG 5 cut(s) 137, 241, 504, 704, 761
MvaI CCWGG 4 cut(s) 137, 241, 704, 761
MwoI GCNNNNNNNGC 2 cut(s) 119, 499
NciI CCSGG 1 cut(s) 504
NdeII GATC 5 cut(s) 346, 616, 622, 658, 787
NlaIII CATG 3 cut(s) 370, 751, 782
NlaIV GGNNCC 2 cut(s) 441, 541
NmeAIII GCCGAG 1 cut(s) 29
NsbI TGCGCA 1 cut(s) 494
NspI RCATGY 1 cut(s) 751
PaeR7I CTCGAG 1 cut(s) 350
PagI TCATGA 2 cut(s) 366, 778
PciI ACATGT 1 cut(s) 747
PciSI GCTCTTC 1 cut(s) 239
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PfeI GAWTC 3 cut(s) 596, 678, 708
PfoI TCCNGGA 1 cut(s) 239
PkrI GCNGC 4 cut(s) 55, 99, 306, 501
PleI GAGTC 2 cut(s) 187, 244
PpsI GAGTC 2 cut(s) 187, 244
PpuMI RGGWCCY 1 cut(s) 540
PscI ACATGT 1 cut(s) 747
Psp5II RGGWCCY 1 cut(s) 540
Psp6I CCWGG 4 cut(s) 135, 239, 702, 759
PspGI CCWGG 4 cut(s) 135, 239, 702, 759
PspN4I GGNNCC 2 cut(s) 441, 541
PspPI GGNCC 3 cut(s) 133, 540, 700
PspPPI RGGWCCY 1 cut(s) 540
RsaI GTAC 3 cut(s) 484, 740, 765
RsaNI GTAC 3 cut(s) 483, 739, 764
RseI CAYNNNNRTG 1 cut(s) 783
SapI GCTCTTC 1 cut(s) 239
SaqAI TTAA 1 cut(s) 92
SatI GCNGC 4 cut(s) 54, 98, 305, 500
Sau3AI GATC 5 cut(s) 346, 616, 622, 658, 787
Sau96I GGNCC 3 cut(s) 133, 540, 700
ScaI AGTACT 1 cut(s) 740
SchI GAGTC 2 cut(s) 187, 245
ScrFI CCNGG 5 cut(s) 137, 241, 504, 704, 761
SduI GDGCHC 2 cut(s) 79, 150
Sfr274I CTCGAG 1 cut(s) 350
SinI GGWCC 2 cut(s) 540, 700
SlaI CTCGAG 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 783
SmlI CTYRAG 3 cut(s) 205, 250, 350
SmoI CTYRAG 3 cut(s) 205, 250, 350
SsiI CCGC 5 cut(s) 122, 169, 175, 304, 419
StyD4I CCNGG 5 cut(s) 135, 239, 502, 702, 759
StyI CCWWGG 1 cut(s) 318
TaqI TCGA 2 cut(s) 68, 351
TaqII GACCGA 2 cut(s) 306, 314
TatI WGTACW 1 cut(s) 738
TauI GCSGC 1 cut(s) 307
TfiI GAWTC 3 cut(s) 596, 678, 708
Tru1I TTAA 1 cut(s) 92
Tru9I TTAA 1 cut(s) 92
TseI GCWGC 3 cut(s) 53, 97, 499
TspDTI ATGAA 2 cut(s) 701, 767
VpaK11BI GGWCC 2 cut(s) 540, 700
XceI RCATGY 1 cut(s) 751
XhoI CTCGAG 1 cut(s) 350
ZrmI AGTACT 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.