Rh2DG310100

tRNA (Guanine-1)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0012259
Physical Location & Seq
Forward (+)
38067441 .. 38069323
1883 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG310100.1

Sequence Viewer

Length: 876 bp
ATGGAGGGCGTAGAAAATGGGGGAAAAGCCACGCAATGTCCGAACTGCTCGGCAGCAGTAGTACTATCGAAGAGTGCCCAGAAGAAGGTGTTGAAGCAGCAGAGGTGGGAGGCGAAGAAGGCGGAGAAGAAGGCCCAGGAAAAAGAGCACAAGAAGAGAGAGGGAGAGCGGAAGCGGAAGGAATGGGAGGAGACTCTGGCGAGCTTGAGCGAAGACGAGAGGTTGAAGATGATAGAGTCCAGGAAGAGCTTGAGGAAGGAGAGAATGGACAAGAGGTCAGAGGAGCGAGACCGAAAGACGGAGCGGCTCACCAGAGCCAAGGAGTGTGGACAGAAGATTGTGATTGATCTCGAGTTCTCTCACCTCATGACGCCCTCTGAGACCCACAGCCTCGTTCAACAGATTATGTATTGTTATGCGGTGAATGGGAGGAGTAGGGAGCCGTGTCATCTGTGGCTGACTGGGTGTAAAGGAGAGATGGGTACGCAGTTGCGCAGGCTGCCGGGGTTTGATAAGTGGAGTGTTGAGAGGGAAGATGGGTCCTACATTGAGGCTTTGGAGAGTGAGAAGAACAATCTGGTGTATCTGACCGCTGATTCGGAAAATGTGGTGGATGATCTTGATCGGAGCAAGATATATATTATTGGGGGATTGGTGGATCGGAATAGGTGGAAAGGAATCACAATGAAGAAAGCAGAGGACCAGGGAATCCAAACTGCGAAGCTCCCCATCGGGAAGTACTTGAACATGTGCAGTTCCCAGGTACTCACTATGATCATTTTTCGGATTGAATTGAGCCCGATTTTTAGAGTGAACATTAAAGTGCGCTTTTGTCTGTTGATGATTGCAGGTCCTGACTGTGAATCAAGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_m1G_MT

Protein Analysis

291

Amino Acids

33.51

Weight (kDa)

9.09

Isoelectric Point (pI)

50.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 494
Acc36I ACCTGC 1 cut(s) 839
AccBSI CCGCTC 2 cut(s) 169, 304
AciI CCGC 6 cut(s) 122, 169, 175, 304, 419, 591
AclWI GGATC 1 cut(s) 666
AcyI GRCGYC 1 cut(s) 371
AfaI GTAC 4 cut(s) 63, 484, 740, 765
AfiI CCNNNNNNNGG 2 cut(s) 85, 298
AflIII ACRYGT 1 cut(s) 747
AgsI TTSAA 5 cut(s) 94, 226, 398, 745, 791
AhdI GACNNNNNGTC 1 cut(s) 274
AjnI CCWGG 4 cut(s) 135, 239, 702, 759
AluBI AGCT 3 cut(s) 204, 249, 724
AluI AGCT 3 cut(s) 204, 249, 724
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 3 cut(s) 185, 282, 374
AlwI GGATC 1 cut(s) 666
AlwNI CAGNNNCTG 1 cut(s) 854
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 1 cut(s) 132
ApeKI GCWGC 3 cut(s) 53, 97, 499
ArsI GACNNNNNNTTYG 2 cut(s) 580, 612
AspLEI GCGC 2 cut(s) 495, 828
AspS9I GGNCC 4 cut(s) 133, 540, 700, 851
AsuC2I CCSGG 1 cut(s) 504
AsuHPI GGTGA 3 cut(s) 301, 353, 433
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 3 cut(s) 540, 700, 851
BaeGI GKGCMC 1 cut(s) 79
BanII GRGCYC 1 cut(s) 800
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 3 cut(s) 65, 109, 486
BccI CCATC 3 cut(s) 472, 530, 737
BceAI ACGGC 1 cut(s) 427
BciT130I CCWGG 4 cut(s) 137, 241, 704, 761
BclI TGATCA 1 cut(s) 774
BcnI CCSGG 1 cut(s) 504
BcoDI GTCTC 3 cut(s) 185, 282, 374
BfuAI ACCTGC 1 cut(s) 839
BisI GCNGC 4 cut(s) 54, 98, 305, 500
BlsI GCNGC 4 cut(s) 55, 99, 306, 501
BmcAI AGTACT 2 cut(s) 63, 740
Bme1390I CCNGG 5 cut(s) 137, 241, 504, 704, 761
Bme18I GGWCC 3 cut(s) 540, 700, 851
BmeRI GACNNNNNGTC 1 cut(s) 274
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 4 cut(s) 133, 540, 700, 851
BmiI GGNNCC 2 cut(s) 441, 541
BmrFI CCNGG 5 cut(s) 137, 241, 504, 704, 761
BmrI ACTGGG 1 cut(s) 471
BmuI ACTGGG 1 cut(s) 471
BpiI GAAGAC 1 cut(s) 219
BpuEI CTTGAG 2 cut(s) 226, 271
BpuMI CCSGG 1 cut(s) 504
BsaBI GATNNNNATC 1 cut(s) 621
BsaHI GRCGYC 1 cut(s) 371
BsaI GGTCTC 2 cut(s) 282, 374
BsaJI CCNNGG 5 cut(s) 135, 318, 503, 703, 759
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 298
Bse1I ACTGG 1 cut(s) 466
Bse3DI GCAATG 1 cut(s) 41
Bse8I GATNNNNATC 1 cut(s) 621
BseBI CCWGG 4 cut(s) 137, 241, 704, 761
BseDI CCNNGG 5 cut(s) 135, 318, 503, 703, 759
BseGI GGATG 1 cut(s) 619
BseJI GATNNNNATC 1 cut(s) 621
BseLI CCNNNNNNNGG 2 cut(s) 85, 298
BseMI GCAATG 1 cut(s) 41
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 1 cut(s) 466
BseRI GAGGAG 3 cut(s) 203, 296, 445
BseSI GKGCMC 1 cut(s) 79
BseXI GCAGC 3 cut(s) 65, 109, 486
BsgI GTGCAG 1 cut(s) 772
BshFI GGCC 1 cut(s) 134
BsiHKAI GWGCWC 1 cut(s) 150
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 1 cut(s) 503
BslI CCNNNNNNNGG 2 cut(s) 85, 298
BsmAI GTCTC 3 cut(s) 185, 282, 374
BsnI GGCC 1 cut(s) 134
Bso31I GGTCTC 2 cut(s) 282, 374
BsoBI CYCGRG 1 cut(s) 350
Bsp1286I GDGCHC 3 cut(s) 79, 150, 800
Bsp143I GATC 5 cut(s) 346, 616, 622, 658, 774
BspACI CCGC 6 cut(s) 122, 169, 175, 304, 419, 591
BspANI GGCC 1 cut(s) 134
BspCNI CTCAG 1 cut(s) 370
BspHI TCATGA 1 cut(s) 366
BspLI GGNNCC 2 cut(s) 441, 541
BspMI ACCTGC 1 cut(s) 839
BspPI GGATC 1 cut(s) 666
BspQI GCTCTTC 1 cut(s) 239
BspTNI GGTCTC 2 cut(s) 282, 374
BsrBI CCGCTC 2 cut(s) 169, 304
BsrDI GCAATG 1 cut(s) 41
BsrI ACTGG 1 cut(s) 466
BssECI CCNNGG 5 cut(s) 135, 318, 503, 703, 759
BssMI GATC 5 cut(s) 346, 616, 622, 658, 774
BssNI GRCGYC 1 cut(s) 371
BssT1I CCWWGG 1 cut(s) 318
Bst2UI CCWGG 4 cut(s) 137, 241, 704, 761
Bst4CI ACNGT 1 cut(s) 860
Bst6I CTCTTC 3 cut(s) 65, 149, 239
BstACI GRCGYC 1 cut(s) 371
BstC8I GCNNGC 2 cut(s) 202, 497
BstDEI CTNAG 1 cut(s) 378
BstF5I GGATG 1 cut(s) 619
BstHHI GCGC 2 cut(s) 495, 828
BstKTI GATC 5 cut(s) 349, 619, 625, 661, 777
BstMAI GTCTC 3 cut(s) 185, 282, 374
BstMBI GATC 5 cut(s) 346, 616, 622, 658, 774
BstMWI GCNNNNNNNGC 2 cut(s) 119, 499
BstNI CCWGG 4 cut(s) 137, 241, 704, 761
BstNSI RCATGY 1 cut(s) 751
BstSCI CCNGG 5 cut(s) 135, 239, 502, 702, 759
BstSLI GKGCMC 1 cut(s) 79
BstV1I GCAGC 3 cut(s) 65, 109, 486
BstV2I GAAGAC 1 cut(s) 219
BsuRI GGCC 1 cut(s) 134
BtsCI GGATG 1 cut(s) 619
BveI ACCTGC 1 cut(s) 839
Cac8I GCNNGC 2 cut(s) 202, 497
CaiI CAGNNNCTG 1 cut(s) 854
CciI TCATGA 1 cut(s) 366
CfoI GCGC 2 cut(s) 495, 828
Cfr13I GGNCC 4 cut(s) 133, 540, 700, 851
CseI GACGC 1 cut(s) 379
Csp6I GTAC 4 cut(s) 62, 483, 739, 764
CspCI CAANNNNNGTGG 2 cut(s) 307, 342
CviAII CATG 2 cut(s) 367, 748
CviQI GTAC 4 cut(s) 62, 483, 739, 764
DdeI CTNAG 1 cut(s) 378
DpnI GATC 5 cut(s) 348, 618, 624, 660, 776
DpnII GATC 5 cut(s) 346, 616, 622, 658, 774
DriI GACNNNNNGTC 1 cut(s) 274
Eam1104I CTCTTC 3 cut(s) 65, 149, 239
Eam1105I GACNNNNNGTC 1 cut(s) 274
EarI CTCTTC 3 cut(s) 65, 149, 239
EciI GGCGGA 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 318
Eco24I GRGCYC 1 cut(s) 800
Eco31I GGTCTC 2 cut(s) 282, 374
Eco47I GGWCC 3 cut(s) 540, 700, 851
Eco88I CYCGRG 1 cut(s) 350
EcoO109I RGGNCCY 2 cut(s) 540, 851
EcoRII CCWGG 4 cut(s) 135, 239, 702, 759
EcoT14I CCWWGG 1 cut(s) 318
EcoT38I GRGCYC 1 cut(s) 800
ErhI CCWWGG 1 cut(s) 318
FaeI CATG 2 cut(s) 370, 751
FaiI YATR 7 cut(s) 368, 407, 417, 637, 639, 749, 773
FatI CATG 2 cut(s) 366, 747
FbaI TGATCA 1 cut(s) 774
Fnu4HI GCNGC 4 cut(s) 54, 98, 305, 500
FokI GGATG 1 cut(s) 626
FriOI GRGCYC 1 cut(s) 800
Fsp4HI GCNGC 4 cut(s) 54, 98, 305, 500
FspI TGCGCA 1 cut(s) 494
GlaI GCGC 2 cut(s) 494, 827
GluI GCNGC 4 cut(s) 54, 98, 305, 500
HaeIII GGCC 1 cut(s) 134
HapII CCGG 1 cut(s) 503
HgaI GACGC 1 cut(s) 379
HhaI GCGC 2 cut(s) 495, 828
Hin1I GRCGYC 1 cut(s) 371
Hin1II CATG 2 cut(s) 370, 751
Hin6I GCGC 2 cut(s) 493, 826
HinP1I GCGC 2 cut(s) 493, 826
HinfI GANTC 6 cut(s) 193, 236, 596, 678, 708, 863
HpaII CCGG 1 cut(s) 503
HphI GGTGA 3 cut(s) 301, 353, 433
Hpy166II GTNNAC 2 cut(s) 329, 814
Hpy188I TCNGA 8 cut(s) 42, 280, 379, 588, 601, 627, 663, 786
Hpy188III TCNNGA 5 cut(s) 350, 367, 620, 733, 854
Hpy8I GTNNAC 2 cut(s) 329, 814
HpyAV CCTTC 5 cut(s) 79, 112, 124, 172, 250
HpyCH4III ACNGT 1 cut(s) 860
HpyCH4V TGCA 2 cut(s) 753, 848
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 499
HpyF3I CTNAG 1 cut(s) 378
Hsp92I GRCGYC 1 cut(s) 371
Hsp92II CATG 2 cut(s) 370, 751
HspAI GCGC 2 cut(s) 493, 826
Ksp22I TGATCA 1 cut(s) 774
Kzo9I GATC 5 cut(s) 346, 616, 622, 658, 774
LguI GCTCTTC 1 cut(s) 239
LmnI GCTCC 5 cut(s) 283, 301, 439, 627, 729
Lsp1109I GCAGC 3 cut(s) 65, 109, 486
MalI GATC 5 cut(s) 348, 618, 624, 660, 776
MbiI CCGCTC 2 cut(s) 169, 304
MboI GATC 5 cut(s) 346, 616, 622, 658, 774
MhlI GDGCHC 3 cut(s) 79, 150, 800
MluCI AATT 1 cut(s) 791
MlyI GAGTC 2 cut(s) 187, 245
MseI TTAA 1 cut(s) 819
MslI CAYNNNNRTG 1 cut(s) 821
MspA1I CMGCKG 1 cut(s) 593
MspI CCGG 1 cut(s) 503
MspR9I CCNGG 5 cut(s) 137, 241, 504, 704, 761
MvaI CCWGG 4 cut(s) 137, 241, 704, 761
MwoI GCNNNNNNNGC 2 cut(s) 119, 499
NciI CCSGG 1 cut(s) 504
NdeII GATC 5 cut(s) 346, 616, 622, 658, 774
NlaIII CATG 2 cut(s) 370, 751
NlaIV GGNNCC 2 cut(s) 441, 541
NmeAIII GCCGAG 1 cut(s) 29
NsbI TGCGCA 1 cut(s) 494
NspI RCATGY 1 cut(s) 751
PaeR7I CTCGAG 1 cut(s) 350
PagI TCATGA 1 cut(s) 366
PciI ACATGT 1 cut(s) 747
PciSI GCTCTTC 1 cut(s) 239
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PfeI GAWTC 4 cut(s) 596, 678, 708, 863
PfoI TCCNGGA 1 cut(s) 239
PkrI GCNGC 4 cut(s) 55, 99, 306, 501
PleI GAGTC 2 cut(s) 187, 244
PpsI GAGTC 2 cut(s) 187, 244
PpuMI RGGWCCY 2 cut(s) 540, 851
PscI ACATGT 1 cut(s) 747
Psp5II RGGWCCY 2 cut(s) 540, 851
Psp6I CCWGG 4 cut(s) 135, 239, 702, 759
PspGI CCWGG 4 cut(s) 135, 239, 702, 759
PspN4I GGNNCC 2 cut(s) 441, 541
PspPI GGNCC 4 cut(s) 133, 540, 700, 851
PspPPI RGGWCCY 2 cut(s) 540, 851
PstNI CAGNNNCTG 1 cut(s) 854
RsaI GTAC 4 cut(s) 63, 484, 740, 765
RsaNI GTAC 4 cut(s) 62, 483, 739, 764
RseI CAYNNNNRTG 1 cut(s) 821
SapI GCTCTTC 1 cut(s) 239
SaqAI TTAA 1 cut(s) 819
SatI GCNGC 4 cut(s) 54, 98, 305, 500
Sau3AI GATC 5 cut(s) 346, 616, 622, 658, 774
Sau96I GGNCC 4 cut(s) 133, 540, 700, 851
ScaI AGTACT 2 cut(s) 63, 740
SchI GAGTC 2 cut(s) 187, 245
ScrFI CCNGG 5 cut(s) 137, 241, 504, 704, 761
SduI GDGCHC 3 cut(s) 79, 150, 800
Sfr274I CTCGAG 1 cut(s) 350
SinI GGWCC 3 cut(s) 540, 700, 851
SlaI CTCGAG 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 821
SmlI CTYRAG 3 cut(s) 205, 250, 350
SmoI CTYRAG 3 cut(s) 205, 250, 350
Sse9I AATT 1 cut(s) 791
SsiI CCGC 6 cut(s) 122, 169, 175, 304, 419, 591
StyD4I CCNGG 5 cut(s) 135, 239, 502, 702, 759
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 1 cut(s) 860
TaqI TCGA 2 cut(s) 68, 351
TaqII GACCGA 1 cut(s) 306
TasI AATT 1 cut(s) 791
TatI WGTACW 2 cut(s) 61, 738
TauI GCSGC 1 cut(s) 307
TfiI GAWTC 4 cut(s) 596, 678, 708, 863
Tru1I TTAA 1 cut(s) 819
Tru9I TTAA 1 cut(s) 819
TseI GCWGC 3 cut(s) 53, 97, 499
TspDTI ATGAA 1 cut(s) 701
TspGWI ACGGA 1 cut(s) 314
VpaK11BI GGWCC 3 cut(s) 540, 700, 851
XceI RCATGY 1 cut(s) 751
XhoI CTCGAG 1 cut(s) 350
ZrmI AGTACT 2 cut(s) 63, 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.