FvH4_1g25600

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0011022
Physical Location & Seq
Forward (+)
17370979 .. 17378543
7565 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25600.t2

Sequence Viewer

Length: 2127 bp
ATGATGGTTGATATAGGCATCTCACTTGCAAGATTTGTGACCAGCTCAGACAGTAATGGTTCATTTCTTCTGGGATGGTTGGTCACAGGATCATTTGGTCTTCTAGCTATCATCTTTGCATTTCTCAAATGGGAGAGAAGGACATCTCTGAATTGGGTTAAGGCTGCGGCTAGAGCAAAGAAGAAAGTCTGGAAGAAGCTAAGAGCTCCTTTATCGCCGCATACGTGGATTGAAGATATAACTACTGGTGAACAGCCATCTACATGCTGTGTTTGCTTGACTTCCTTGGTGTTTCTTCAAAACCTAGGCACTAAAGCTTCATCGCACACTCCTGTTCATCGTTGTGTTGTTTGTGGGGTGGCAGCTCATTCTTATTGTTCTGAGTTTGCAGTAAAAGATTGCAAGTGTGTGGCACAAGCTGGTTCTACCCATGTTCGACATCACTGGTCAGAAAGATGGGTTAATATAGATGATAATCCTGAAATTTCCGCCTGCTGCTTTTATTGTGATGAGCCATGTGGTGTTCCGTTCCTTGATGCTTCTCCAACATGGCATTGTCTATGGTGTCAGAACCTCATACATGTCAGATGTCACGATAAAATGGCAAAAGAGTCTGGTGATGTTTGTGATCTTGGTACTCTTAGGAGGGTTATTCTTTCTCCTTTATGTGTAAAAGAAATTGGTCCCTTAAATAGAGATGGAATGCTGCGTGCAATAACAGATGAGTTTATAACCTCATCTGTTCGTGGCCAGTTTAGAAGAAAGCGTCATCGTTCTAAACATGGTAGTGGTCATTCAGGTAATTGTAACTCATCCAATGATGCTCTGGAGTATGCATTCAATGGGCTTACTGGTTTGAAGAAATCCCGTAGTGAGAGGAGTTTTGACCACTTGAAGAAGGATGGTAGGGCGCTAAGTGTTAAGGGTAACCATAATGGGATTATGCAGAAAAATGGTGGTATTGTAACCTACGGTCAATTCAAGAAATATAAAGTGGTTAATTTACCAAAGGATGCGAGGCCTCTTTTGGTTTTTATAAATGCCAGGAGTGGAGGTCAAAATGGGGCTTCTTTAAGGAGGAGGTTGAACATGTTATTGAATCCTGCGCAGGTTTTTGAATTGAGTTCTTCTCATGGGCCTGAGGTTGGTTTGGAATTGTTCAATGATGTGCAATACTTTAGAGTTTTGGTATGTGGTGGAGATGGCACTGTTGCATGGGTCCTTGATGCCATTGAGAAGCATAAATTTGAATCACCCCCACCTGTTGCTATTATTCCCCTTGGCACAGGAAATGATTTGTCAAGGGTTCTGCAATGGGGAAGAGGCTTCTCTATCGTTAATGGACAAGGTGGCTTAACCACGCTTCTACATGACATCAACCATGCTGCAGTTACCATGCTAGATCGCTGGAAGGTAAACATAAAATCAGAAGCTGATCCAAATAAAGTGCAATCCAAGTTTATGATGAACTATTTAGGTATTGGATGTGATGCAAAGGTTGCTTATGAATTTCATGTGATGCGAGAAATAAATCCTGAGAAGTTCAGTAGTCAGTTTGTGAATAAACTGCGATATGCCAAAGAAGGAGCTAGAGATATAATGGACCGAACTTGTGCCGACTTACCATGGCAAGTTTGGCTTGAAGTTGATGGGAAGGACATTGAAATTCCAAAGGATTCTGAGGGTCTAATTGTGCTCAACATTGGCAGCTACATGGGTGGAGTAGATCTTTGGCAAAATGATATTGATCATGATGATGACTTTAGTCTCCAGTCAATGCATGATAAAATGCTTGAAGTTGTCTGCATATGTGGATCGTGGCACCTTGGCAAACTACAGGTTGGACTATCACAAGCTAGGAGACTAGCCCAGGGGAATATCATAAGAATACATGCATCTAGTCCCTTTCCGGTCCAAATAGATGGGGAGCCATATATACAGCAACCTGGCTGTATAGAGATAACACATCATGGACAGGTGTTTATGTTGAGGAGGGCTTCAGAGGAGCCGAGAGGGCATGCAGCTGCAATCATGGCGGAGGTTTTATTAGACGCTGAGTGTAAAGGCATCATAAATGCGTCTCAGAAGAAAACACTACTCCAGCAGATGGCTCTCCAGCTTGCTTAA

Protein Analysis

709

Amino Acids

78.74

Weight (kDa)

8.6

Isoelectric Point (pI)

48.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C1_1 PF00130 147 - 206 7.6e-07 Phorbol esters/diacylglycerol binding domain (C1 domain)
DAGK_cat PF00781 341 - 441 1.5e-26 Diacylglycerol kinase catalytic domain
DAGK_acc PF00609 487 - 644 8.4e-58 Diacylglycerol kinase accessory domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011022)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63770 AT5G63770
fragaria_vesca FvH4_1g25600 FvH4_1g25600 FvH4_1g25600
malus_domestica MD15G1139400.v1.1
prunus_persica Prupe.4G282200_v2.0.a1 Prupe.4G282200_v2.0.a1 Prupe.4G282200_v2.0.a1
pyrus_communis pycom15g12580
rosa_chinensis RchiOBHm_Chr2g0123771
rosa_laevigata RLG00000018775
rosa_multiflora Rmu_sc0001032.1_g000003
rosa_roxburghii Rroxscaffold_2G00120350
rosa_rugosa Rorug02G0247900
rosa_samantha Rh2AG306800 Rh2BG315300 Rh2CG293200 Rh2DG330500
rosa_wichuraiana Rw2G024620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 731, 1037
Acc16I TGCGCA 1 cut(s) 1107
Acc36I ACCTGC 1 cut(s) 1099
AccB1I GGYRCC 1 cut(s) 1821
AccB7I CCANNNNNTGG 1 cut(s) 2107
AciI CCGC 4 cut(s) 167, 218, 489, 2036
AclWI GGATC 3 cut(s) 97, 1430, 1822
AcoI YGGCCR 1 cut(s) 748
AcsI RAATTY 4 cut(s) 483, 1244, 1508, 1665
AcuI CTGAAG 1 cut(s) 1983
AfaI GTAC 1 cut(s) 637
AfiI CCNNNNNNNGG 2 cut(s) 1145, 2107
AflIII ACRYGT 2 cut(s) 580, 1089
AjnI CCWGG 3 cut(s) 1043, 1869, 1945
Alw21I GWGCWC 2 cut(s) 208, 1698
Alw26I GTCTC 3 cut(s) 1772, 1855, 2085
AlwI GGATC 3 cut(s) 97, 1430, 1822
AlwNI CAGNNNCTG 1 cut(s) 1433
AoxI GGCC 3 cut(s) 748, 1019, 1136
ApeKI GCWGC 8 cut(s) 164, 362, 495, 706, 1385, 1707, 2021, 2024
ApoI RAATTY 4 cut(s) 483, 1244, 1508, 1665
AspA2I CCTAGG 1 cut(s) 304
AspLEI GCGC 2 cut(s) 913, 1108
AspS9I GGNCC 5 cut(s) 683, 1136, 1219, 1603, 1912
AsuHPI GGTGA 3 cut(s) 260, 629, 1245
AvaII GGWCC 4 cut(s) 683, 1219, 1603, 1912
AvrII CCTAGG 1 cut(s) 304
AxyI CCTNAGG 1 cut(s) 1140
BalI TGGCCA 1 cut(s) 750
BanI GGYRCC 1 cut(s) 1821
BanII GRGCYC 1 cut(s) 208
BbsI GAAGAC 1 cut(s) 92
Bbv12I GWGCWC 2 cut(s) 208, 1698
BbvI GCAGC 8 cut(s) 151, 374, 482, 693, 1372, 1719, 2011, 2033
BccI CCATC 9 cut(s) 69, 265, 450, 692, 896, 1196, 1643, 1916, 2101
BciT130I CCWGG 3 cut(s) 1045, 1871, 1947
BclI TGATCA 1 cut(s) 1747
BcoDI GTCTC 3 cut(s) 1772, 1855, 2085
BfaI CTAG 8 cut(s) 104, 171, 305, 1400, 1590, 1857, 1865, 1899
BfmI CTRYAG 2 cut(s) 1386, 1835
BfoI RGCGCY 1 cut(s) 914
BfuAI ACCTGC 1 cut(s) 1099
BglI GCCNNNNNGGC 1 cut(s) 2014
BglII AGATCT 1 cut(s) 1726
BlnI CCTAGG 1 cut(s) 304
Bme1390I CCNGG 3 cut(s) 1045, 1871, 1947
Bme18I GGWCC 4 cut(s) 683, 1219, 1603, 1912
BmgT120I GGNCC 5 cut(s) 683, 1136, 1219, 1603, 1912
BmiI GGNNCC 5 cut(s) 685, 1220, 1823, 1929, 2007
BmrFI CCNGG 3 cut(s) 1045, 1871, 1947
BmsI GCATC 9 cut(s) 27, 526, 811, 1003, 1216, 1480, 1509, 1904, 2076
BoxI GACNNNNGTC 1 cut(s) 1764
BpiI GAAGAC 1 cut(s) 92
BplI GAGNNNNNCTC 2 cut(s) 1114, 1146
BpmI CTGGAG 4 cut(s) 848, 1754, 2084, 2099
BsaAI YACGTR 1 cut(s) 225
BsaBI GATNNNNATC 2 cut(s) 474, 1746
BsaJI CCNNGG 7 cut(s) 285, 304, 1279, 1625, 1825, 1869, 1870
BsaWI WCCGGW 1 cut(s) 1909
BsaXI ACNNNNNCTCC 2 cut(s) 1039, 1069
Bsc4I CCNNNNNNNGG 2 cut(s) 1145, 2107
Bse1I ACTGG 5 cut(s) 250, 449, 751, 856, 1771
Bse21I CCTNAGG 1 cut(s) 1140
Bse3DI GCAATG 1 cut(s) 1319
Bse8I GATNNNNATC 2 cut(s) 474, 1746
BseBI CCWGG 3 cut(s) 1045, 1871, 1947
BseDI CCNNGG 7 cut(s) 285, 304, 1279, 1625, 1825, 1869, 1870
BseGI GGATG 5 cut(s) 80, 812, 907, 1018, 1490
BseJI GATNNNNATC 2 cut(s) 474, 1746
BseLI CCNNNNNNNGG 2 cut(s) 1145, 2107
BseMI GCAATG 1 cut(s) 1319
BseMII CTCAG 7 cut(s) 60, 372, 1131, 1527, 1671, 2046, 2096
BseNI ACTGG 5 cut(s) 250, 449, 751, 856, 1771
BseRI GAGGAG 4 cut(s) 892, 1093, 2005, 2018
BseXI GCAGC 8 cut(s) 151, 374, 482, 693, 1372, 1719, 2011, 2033
BshFI GGCC 3 cut(s) 750, 1021, 1138
BshNI GGYRCC 1 cut(s) 1821
BsiHKAI GWGCWC 2 cut(s) 208, 1698
BsiSI CCGG 1 cut(s) 1910
BslFI GGGAC 2 cut(s) 669, 1887
BslI CCNNNNNNNGG 2 cut(s) 1145, 2107
BsmAI GTCTC 3 cut(s) 1772, 1855, 2085
BsmBI CGTCTC 1 cut(s) 2085
BsmFI GGGAC 2 cut(s) 669, 1887
BsmI GAATGC 2 cut(s) 708, 836
BsnI GGCC 3 cut(s) 750, 1021, 1138
Bsp1286I GDGCHC 2 cut(s) 208, 1698
Bsp143I GATC 7 cut(s) 89, 628, 1402, 1435, 1726, 1747, 1814
Bsp19I CCATGG 1 cut(s) 1625
BspACI CCGC 4 cut(s) 167, 218, 489, 2036
BspANI GGCC 3 cut(s) 750, 1021, 1138
BspCNI CTCAG 7 cut(s) 59, 373, 1132, 1528, 1672, 2047, 2095
BspHI TCATGA 1 cut(s) 1750
BspLI GGNNCC 5 cut(s) 685, 1220, 1823, 1929, 2007
BspMAI CTGCAG 1 cut(s) 1390
BspMI ACCTGC 1 cut(s) 1099
BspPI GGATC 3 cut(s) 97, 1430, 1822
BspT107I GGYRCC 1 cut(s) 1821
BsrDI GCAATG 1 cut(s) 1319
BsrI ACTGG 5 cut(s) 250, 449, 751, 856, 1771
BssECI CCNNGG 7 cut(s) 285, 304, 1279, 1625, 1825, 1869, 1870
BssMI GATC 7 cut(s) 89, 628, 1402, 1435, 1726, 1747, 1814
BssT1I CCWWGG 5 cut(s) 285, 304, 1279, 1625, 1825
Bst2UI CCWGG 3 cut(s) 1045, 1871, 1947
Bst4CI ACNGT 3 cut(s) 53, 974, 1210
Bst6I CTCTTC 1 cut(s) 1315
BstAPI GCANNNNNTGC 1 cut(s) 1499
BstBAI YACGTR 1 cut(s) 225
BstC8I GCNNGC 4 cut(s) 493, 711, 2019, 2121
BstDSI CCRYGG 1 cut(s) 1625
BstEII GGTNACC 1 cut(s) 926
BstF5I GGATG 5 cut(s) 80, 812, 907, 1018, 1490
BstH2I RGCGCY 1 cut(s) 914
BstHHI GCGC 2 cut(s) 913, 1108
BstKTI GATC 7 cut(s) 92, 631, 1405, 1438, 1729, 1750, 1817
BstMAI GTCTC 3 cut(s) 1772, 1855, 2085
BstMBI GATC 7 cut(s) 89, 628, 1402, 1435, 1726, 1747, 1814
BstMWI GCNNNNNNNGC 6 cut(s) 173, 273, 1499, 1636, 2014, 2033
BstNI CCWGG 3 cut(s) 1045, 1871, 1947
BstNSI RCATGY 5 cut(s) 267, 584, 1093, 1895, 2021
BstPAI GACNNNNGTC 1 cut(s) 1764
BstPI GGTNACC 1 cut(s) 926
BstSCI CCNGG 3 cut(s) 1043, 1869, 1945
BstSFI CTRYAG 2 cut(s) 1386, 1835
BstV1I GCAGC 8 cut(s) 151, 374, 482, 693, 1372, 1719, 2011, 2033
BstV2I GAAGAC 1 cut(s) 92
BstX2I RGATCY 1 cut(s) 1726
BstXI CCANNNNNNTGG 1 cut(s) 1922
BstYI RGATCY 1 cut(s) 1726
Bsu36I CCTNAGG 1 cut(s) 1140
BsuRI GGCC 3 cut(s) 750, 1021, 1138
BtgI CCRYGG 1 cut(s) 1625
BtgZI GCGATG 1 cut(s) 306
BtsCI GGATG 5 cut(s) 80, 812, 907, 1018, 1490
BtsIMutI CAGTG 2 cut(s) 442, 1206
BveI ACCTGC 1 cut(s) 1099
Cac8I GCNNGC 4 cut(s) 493, 711, 2019, 2121
CaiI CAGNNNCTG 1 cut(s) 1433
CciI TCATGA 1 cut(s) 1750
CfoI GCGC 2 cut(s) 913, 1108
Cfr13I GGNCC 5 cut(s) 683, 1136, 1219, 1603, 1912
CseI GACGC 3 cut(s) 755, 2060, 2067
Csp6I GTAC 1 cut(s) 636
CviQI GTAC 1 cut(s) 636
DpnI GATC 7 cut(s) 91, 630, 1404, 1437, 1728, 1749, 1816
DpnII GATC 7 cut(s) 89, 628, 1402, 1435, 1726, 1747, 1814
EaeI YGGCCR 1 cut(s) 748
Eam1104I CTCTTC 1 cut(s) 1315
EarI CTCTTC 1 cut(s) 1315
EciI GGCGGA 2 cut(s) 478, 2051
Ecl136II GAGCTC 1 cut(s) 206
Eco130I CCWWGG 5 cut(s) 285, 304, 1279, 1625, 1825
Eco147I AGGCCT 1 cut(s) 1021
Eco24I GRGCYC 1 cut(s) 208
Eco47I GGWCC 4 cut(s) 683, 1219, 1603, 1912
Eco53kI GAGCTC 1 cut(s) 206
Eco57I CTGAAG 1 cut(s) 1983
Eco81I CCTNAGG 1 cut(s) 1140
Eco91I GGTNACC 1 cut(s) 926
EcoICRI GAGCTC 1 cut(s) 206
EcoO109I RGGNCCY 1 cut(s) 1219
EcoO65I GGTNACC 1 cut(s) 926
EcoRII CCWGG 3 cut(s) 1043, 1869, 1945
EcoT14I CCWWGG 5 cut(s) 285, 304, 1279, 1625, 1825
EcoT22I ATGCAT 3 cut(s) 838, 1782, 1897
EcoT38I GRGCYC 1 cut(s) 208
ErhI CCWWGG 5 cut(s) 285, 304, 1279, 1625, 1825
Esp3I CGTCTC 1 cut(s) 2085
FalI AAGNNNNNCTT 4 cut(s) 193, 225, 1623, 1655
FaqI GGGAC 2 cut(s) 669, 1887
FauNDI CATATG 1 cut(s) 1808
FbaI TGATCA 1 cut(s) 1747
FokI GGATG 5 cut(s) 87, 799, 914, 1025, 1497
FriOI GRGCYC 1 cut(s) 208
FspBI CTAG 8 cut(s) 104, 171, 305, 1400, 1590, 1857, 1865, 1899
FspI TGCGCA 1 cut(s) 1107
GlaI GCGC 2 cut(s) 912, 1107
GsuI CTGGAG 4 cut(s) 848, 1754, 2084, 2099
HaeII RGCGCY 1 cut(s) 914
HaeIII GGCC 3 cut(s) 750, 1021, 1138
HapII CCGG 1 cut(s) 1910
HgaI GACGC 3 cut(s) 755, 2060, 2067
HhaI GCGC 2 cut(s) 913, 1108
Hin6I GCGC 2 cut(s) 911, 1106
HinP1I GCGC 2 cut(s) 911, 1106
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 4 cut(s) 611, 1099, 1250, 1676
HpaII CCGG 1 cut(s) 1910
HphI GGTGA 3 cut(s) 260, 629, 1245
Hpy166II GTNNAC 2 cut(s) 251, 1417
Hpy188III TCNNGA 7 cut(s) 190, 479, 593, 827, 982, 1535, 1751
Hpy8I GTNNAC 2 cut(s) 251, 1417
HpyAV CCTTC 5 cut(s) 132, 892, 1405, 1577, 1648
HpyCH4III ACNGT 3 cut(s) 53, 974, 1210
HpyCH4IV ACGT 1 cut(s) 224
HpyF10VI GCNNNNNNNGC 6 cut(s) 173, 273, 1499, 1636, 2014, 2033
HpySE526I ACGT 1 cut(s) 224
HspAI GCGC 2 cut(s) 911, 1106
Ksp22I TGATCA 1 cut(s) 1747
Kzo9I GATC 7 cut(s) 89, 628, 1402, 1435, 1726, 1747, 1814
LmnI GCTCC 4 cut(s) 211, 1586, 1927, 2005
Lsp1109I GCAGC 8 cut(s) 151, 374, 482, 693, 1372, 1719, 2011, 2033
LweI GCATC 9 cut(s) 27, 526, 811, 1003, 1216, 1480, 1509, 1904, 2076
MaeI CTAG 8 cut(s) 104, 171, 305, 1400, 1590, 1857, 1865, 1899
MaeII ACGT 1 cut(s) 224
MaeIII GTNAC 7 cut(s) 37, 82, 590, 806, 926, 964, 1390
MalI GATC 7 cut(s) 91, 630, 1404, 1437, 1728, 1749, 1816
MboI GATC 7 cut(s) 89, 628, 1402, 1435, 1726, 1747, 1814
MflI RGATCY 1 cut(s) 1726
MhlI GDGCHC 2 cut(s) 208, 1698
MlsI TGGCCA 1 cut(s) 750
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 1 cut(s) 620
MmeI TCCRAC 2 cut(s) 569, 1822
Mox20I TGGCCA 1 cut(s) 750
Mph1103I ATGCAT 3 cut(s) 838, 1782, 1897
MscI TGGCCA 1 cut(s) 750
MseI TTAA 9 cut(s) 159, 462, 689, 921, 999, 1073, 1338, 1355, 2125
MslI CAYNNNNRTG 4 cut(s) 262, 342, 786, 1755
Msp20I TGGCCA 1 cut(s) 750
MspA1I CMGCKG 1 cut(s) 2024
MspI CCGG 1 cut(s) 1910
MspR9I CCNGG 3 cut(s) 1045, 1871, 1947
Mva1269I GAATGC 2 cut(s) 708, 836
MvaI CCWGG 3 cut(s) 1045, 1871, 1947
MwoI GCNNNNNNNGC 6 cut(s) 173, 273, 1499, 1636, 2014, 2033
NcoI CCATGG 1 cut(s) 1625
NdeI CATATG 1 cut(s) 1808
NdeII GATC 7 cut(s) 89, 628, 1402, 1435, 1726, 1747, 1814
NlaIV GGNNCC 5 cut(s) 685, 1220, 1823, 1929, 2007
NmeAIII GCCGAG 1 cut(s) 2034
NmuCI GTSAC 3 cut(s) 37, 82, 590
NsbI TGCGCA 1 cut(s) 1107
NsiI ATGCAT 3 cut(s) 838, 1782, 1897
NspI RCATGY 5 cut(s) 267, 584, 1093, 1895, 2021
PaeI GCATGC 1 cut(s) 2021
PagI TCATGA 1 cut(s) 1750
PasI CCCWGGG 1 cut(s) 1870
PceI AGGCCT 1 cut(s) 1021
PciI ACATGT 2 cut(s) 580, 1089
PcsI WCGNNNNNNNCGW 1 cut(s) 221
PctI GAATGC 2 cut(s) 708, 836
PfeI GAWTC 3 cut(s) 1099, 1250, 1676
PflMI CCANNNNNTGG 1 cut(s) 2107
PleI GAGTC 1 cut(s) 619
PpsI GAGTC 1 cut(s) 619
Ppu21I YACGTR 1 cut(s) 225
PpuMI RGGWCCY 1 cut(s) 1219
PscI ACATGT 2 cut(s) 580, 1089
PshAI GACNNNNGTC 1 cut(s) 1764
PsiI TTATAA 2 cut(s) 731, 1037
Psp124BI GAGCTC 1 cut(s) 208
Psp5II RGGWCCY 1 cut(s) 1219
Psp6I CCWGG 3 cut(s) 1043, 1869, 1945
PspEI GGTNACC 1 cut(s) 926
PspGI CCWGG 3 cut(s) 1043, 1869, 1945
PspN4I GGNNCC 5 cut(s) 685, 1220, 1823, 1929, 2007
PspPI GGNCC 5 cut(s) 683, 1136, 1219, 1603, 1912
PspPPI RGGWCCY 1 cut(s) 1219
PstI CTGCAG 1 cut(s) 1390
PstNI CAGNNNCTG 1 cut(s) 1433
PsuI RGATCY 1 cut(s) 1726
PvuII CAGCTG 1 cut(s) 2024
RsaI GTAC 1 cut(s) 637
RsaNI GTAC 1 cut(s) 636
RseI CAYNNNNRTG 4 cut(s) 262, 342, 786, 1755
SacI GAGCTC 1 cut(s) 208
SaqAI TTAA 9 cut(s) 159, 462, 689, 921, 999, 1073, 1338, 1355, 2125
Sau3AI GATC 7 cut(s) 89, 628, 1402, 1435, 1726, 1747, 1814
Sau96I GGNCC 5 cut(s) 683, 1136, 1219, 1603, 1912
SchI GAGTC 1 cut(s) 620
ScrFI CCNGG 3 cut(s) 1045, 1871, 1947
SduI GDGCHC 2 cut(s) 208, 1698
SfaNI GCATC 9 cut(s) 27, 526, 811, 1003, 1216, 1480, 1509, 1904, 2076
SfcI CTRYAG 2 cut(s) 1386, 1835
SinI GGWCC 4 cut(s) 683, 1219, 1603, 1912
SmiMI CAYNNNNRTG 4 cut(s) 262, 342, 786, 1755
SphI GCATGC 1 cut(s) 2021
SseBI AGGCCT 1 cut(s) 1021
SsiI CCGC 4 cut(s) 167, 218, 489, 2036
SspMI CTAG 8 cut(s) 104, 171, 305, 1400, 1590, 1857, 1865, 1899
SstI GAGCTC 1 cut(s) 208
StuI AGGCCT 1 cut(s) 1021
StyD4I CCNGG 3 cut(s) 1043, 1869, 1945
StyI CCWWGG 5 cut(s) 285, 304, 1279, 1625, 1825
TaaI ACNGT 3 cut(s) 53, 974, 1210
TaiI ACGT 1 cut(s) 227
TaqI TCGA 1 cut(s) 436
TaqII GACCGA 1 cut(s) 1620
TauI GCSGC 2 cut(s) 170, 220
TfiI GAWTC 3 cut(s) 1099, 1250, 1676
Tru1I TTAA 9 cut(s) 159, 462, 689, 921, 999, 1073, 1338, 1355, 2125
Tru9I TTAA 9 cut(s) 159, 462, 689, 921, 999, 1073, 1338, 1355, 2125
TscAI CASTG 2 cut(s) 449, 1213
TseFI GTSAC 3 cut(s) 37, 82, 590
TseI GCWGC 8 cut(s) 164, 362, 495, 706, 1385, 1707, 2021, 2024
Tsp45I GTSAC 3 cut(s) 37, 82, 590
TspDTI ATGAA 6 cut(s) 51, 309, 326, 1481, 1502, 1521
TspGWI ACGGA 1 cut(s) 516
TspRI CASTG 2 cut(s) 449, 1213
Van91I CCANNNNNTGG 1 cut(s) 2107
VpaK11BI GGWCC 4 cut(s) 683, 1219, 1603, 1912
XapI RAATTY 4 cut(s) 483, 1244, 1508, 1665
XceI RCATGY 5 cut(s) 267, 584, 1093, 1895, 2021
XcmI CCANNNNNNNNNTGG 2 cut(s) 823, 1632
XmaJI CCTAGG 1 cut(s) 304
XspI CTAG 8 cut(s) 104, 171, 305, 1400, 1590, 1857, 1865, 1899
Zsp2I ATGCAT 3 cut(s) 838, 1782, 1897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.