Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011022

Physical Location & Seq

Genomic Coordinates Forward (+)

10211320 .. 10217232

Gene Structure 5913 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1139400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2127 bp

ATGATGGTTGATTTGGGCCTTTCACTTGCTAGGTTTGTGACCGCTGCGGATGGTTATGGTCCGTTTCTCTTAGGGTGGCTGGTCACTGGGTCATTCGGGCTTGTAGCTATCATCTTTGCTTTTCTTAAATGGCAGAGAAGGACATCTCTAAAATGGGTTAAAGCTGCAGCTAGAGCAAAAAAGAAAGTCTGGAAGAAGCTAAAAGTTCCATTATCACATCATACGTGGATCGAAGATATTACTAACGGTGAACAGCCATCCACATGTTGTGTTTGCTTGACTTCTTTAGTGTTTCTTCAATACCTAGGTACAAAAGCCTCATCGCGTACTCCTGTCCATCGTTGCTCTGTTTGTGGTGTAGCATCTCATTTCTATTGTTCCCAGTATGCAGTAAAGGATTGCAAGTGTATGGCACAAGCTGGTTTTACCTATGTTCGACACCACTGGTCAGAAAGATGGGTTAATATGCATGATAATCCTGAGATATCGGCCATTTGTTTTTATTGTGATGAGCCATGTGGTGTTCCTTTCCTCGATGCTTCTCCTACATGGCATTGCCTATGGTGTCAGCGCCTCATACATGTCAGATGTCACAACAAAATGGCAAAAGAATATGGCGATGCTTGCGATTTGGGTAGTCTTCGGAGGATTATCATTTCCCCTCTGTGTGTGAAAGAAGTTGGCCAATATAATGGACGTGGAATGCTAAGTTCATTCACAGATGAAATAATAACCTCATCTGTTTGTGGCCAGTATAGAAGAAAGCGCCACCACTGTAAGCATGGTAGTCAGGCAACTAATGGTACCTCTGCCACTGATGCATCTCCGGATTATCTGTTCAATGGATTTACTGGTATAAAGAGATCCCGTAGTGAGAAGAGTTTTGACTACATAAAGAAGAATGATAGGACACTGAGTTTGAAGGGCAACCATAATGGGTTTTTTCAGAATAAGGGGGACACTGTCTCCTATGGTCAAATTAAGAGATATAAAGTAGTTGATTTGCCACATGATGCAAGACCTCTTTTGGTCTTTATCAATTCCAGAAGTGGAGGTCAACGTGGGGCTTCTCTAAGGAGAAGGCTGAACATGCTGTTGAATCCTGTACAGGTTTTTGAACTGAGTTCTTCCCAAGGGCCTGAGGCAGGTTTGGAATTGTTCAATGATGTGCAATATTTTAGAGTTTTGGTATGTGGTGGAGATGGTACTGTTGCATGGGTTCTTGATGCGATCGAGAGGCATAATTTTGAATCACCCCCACCTGTTGCTATTCTTCCCCTAGGTACGGGAAATGATTTGTCAAGGGTGTTGCAATGGGGAAAAGGCTTCTCTATGGTTGATGGACAAGGTGGCTTAACCGCGCTGTTGCATGACATTAACCATGCTGCAGTTACCATGCTAGATCGCTGGAAGGTAAACATCAAATCAGAAGCTGATCCGAATAAAGTTCAGTCCAAGTATATGATGAACTATTTAGGTATTGGATGTGATGCGAAGGTTGCATATGAATTTCATGTGACCCGAGAAATAAATCCTGAGAAGTTCAGTAGTCAGTTTGTAAATAAATTACGATATGCCAAAGAAGGGGCCAGAGATATAATGGACCGAACCTGTGCTGACCTACCATGGCAAGTTTGGCTTGAAGTTGATGGGAAAGACATAGATATTCCAAAGGATTCTGAGGGTCTAATTGTGCTCAACATTGGCAGCTACATGGGTGGAGTAAATCTTTGGCAAAACGATATTGAGCATGATGATGAATTCAGTCTTCAGTTGATGCATGATAAAATGCTTGAAGTTGTATGTGTATGTGGAGCTTGGCACCTTGGAAAACTTCAGGTTGGACTTTCACAAGCTAGAAGACTAGCCCAAGGGAAAGTCATAAGAATACATGCCTCCAGTCCTTTCCCAGTCCAAATAGATGGAGAACCATATATACAACAACCTGGCTGCTTGGAGATTACACATCACGGACAGATGTTCATGTTGAGGAGAGCTTCAGAAGAGCCCAGAGGGCATGCGGCTGCAATTATGACCGACGTTTTACTAGATGCCGAGTGCAAAGGCATCATCAATGCATCCCAGAAGAAAATACTTCTCCAGCAGATGGCCCTCACGCTCACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004143 GO:0006793 GO:0006796 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009611 GO:0009628 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0022622 GO:0032501 GO:0032502 GO:0044237 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

C1_1 DAGK_acc DAGK_cat

Protein Analysis

709

Amino Acids

79.42

Weight (kDa)

8.74

Isoelectric Point (pI)

48.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C1_1	PF00130	147 - 206	5.1e-07	Phorbol esters/diacylglycerol binding domain (C1 domain)
DAGK_cat	PF00781	341 - 441	3.6e-26	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	488 - 644	2.2e-57	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011022)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63770 AT5G63770
fragaria_vesca	FvH4_1g25600 FvH4_1g25600 FvH4_1g25600
malus_domestica	MD15G1139400.v1.1
prunus_persica	Prupe.4G282200_v2.0.a1 Prupe.4G282200_v2.0.a1 Prupe.4G282200_v2.0.a1
pyrus_communis	pycom15g12580
rosa_chinensis	RchiOBHm_Chr2g0123771
rosa_laevigata	RLG00000018775
rosa_multiflora	Rmu_sc0001032.1_g000003
rosa_roxburghii	Rroxscaffold_2G00120350
rosa_rugosa	Rorug02G0247900
rosa_samantha	Rh2AG306800 Rh2BG315300 Rh2CG293200 Rh2DG330500
rosa_wichuraiana	Rw2G024620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1136
Acc65I	GGTACC	1 cut(s)	803
AccB1I	GGYRCC	2 cut(s)	803, 1821
AccB7I	CCANNNNNTGG	1 cut(s)	2107
AccII	CGCG	2 cut(s)	325, 1361
AccIII	TCCGGA	1 cut(s)	826
AciI	CCGC	4 cut(s)	42, 47, 1359, 2021
AclWI	GGATC	3 cut(s)	236, 858, 1430
AcoI	YGGCCR	3 cut(s)	489, 682, 748
AcsI	RAATTY	2 cut(s)	1508, 1760
AcuI	CTGAAG	3 cut(s)	1754, 1820, 1983
AfaI	GTAC	6 cut(s)	310, 328, 805, 1107, 1207, 1285
AfiI	CCNNNNNNNGG	4 cut(s)	1145, 1285, 1584, 2107
AflIII	ACRYGT	2 cut(s)	263, 580
AgsI	TTSAA	9 cut(s)	299, 841, 922, 1099, 1118, 1162, 1250, 1643, 1796
AjiI	CACGTC	1 cut(s)	698
AjnI	CCWGG	1 cut(s)	1945
AloI	GAACNNNNNNTCC	2 cut(s)	821, 853
Alw21I	GWGCWC	1 cut(s)	1698
Alw26I	GTCTC	1 cut(s)	970
AlwI	GGATC	3 cut(s)	236, 858, 1430
AlwNI	CAGNNNCTG	1 cut(s)	1433
Ama87I	CYCGRG	1 cut(s)	1521
Aor13HI	TCCGGA	1 cut(s)	826
AoxI	GGCC	7 cut(s)	16, 489, 682, 748, 1136, 1587, 2109
ApeKI	GCWGC	7 cut(s)	44, 164, 167, 1385, 1707, 1950, 2024
ApoI	RAATTY	2 cut(s)	1508, 1760
ArsI	GACNNNNNNTTYG	2 cut(s)	901, 933
Asp718I	GGTACC	1 cut(s)	803
AspA2I	CCTAGG	2 cut(s)	304, 1279
AspLEI	GCGC	3 cut(s)	573, 768, 1363
AspS9I	GGNCC	6 cut(s)	16, 59, 1136, 1587, 1603, 2110
AsuHPI	GGTGA	2 cut(s)	260, 1245
AvaI	CYCGRG	1 cut(s)	1521
AvaII	GGWCC	2 cut(s)	59, 1603
AvrII	CCTAGG	2 cut(s)	304, 1279
AxyI	CCTNAGG	1 cut(s)	1140
BalI	TGGCCA	2 cut(s)	684, 750
BanI	GGYRCC	2 cut(s)	803, 1821
BanII	GRGCYC	1 cut(s)	2010
BbsI	GAAGAC	3 cut(s)	632, 1760, 1867
Bbv12I	GWGCWC	1 cut(s)	1698
BbvI	GCAGC	7 cut(s)	31, 151, 179, 1372, 1719, 1937, 2011
BccI	CCATC	9 cut(s)	44, 265, 345, 450, 1196, 1334, 1643, 1916, 2101
BciT130I	CCWGG	1 cut(s)	1947
BcoDI	GTCTC	1 cut(s)	970
BfaI	CTAG	8 cut(s)	30, 171, 305, 1280, 1400, 1857, 1865, 2048
BfmI	CTRYAG	2 cut(s)	165, 1386
BfoI	RGCGCY	2 cut(s)	574, 769
BfuAI	ACCTGC	1 cut(s)	1136
BglI	GCCNNNNNGGC	1 cut(s)	2014
BisI	GCNGC	8 cut(s)	45, 165, 168, 1386, 1708, 1951, 2022, 2025
BlnI	CCTAGG	2 cut(s)	304, 1279
BlsI	GCNGC	8 cut(s)	46, 166, 169, 1387, 1709, 1952, 2023, 2026
Bme1390I	CCNGG	1 cut(s)	1947
Bme18I	GGWCC	2 cut(s)	59, 1603
BmeT110I	CYCGRG	1 cut(s)	1521
BmgBI	CACGTC	1 cut(s)	698
BmgT120I	GGNCC	6 cut(s)	16, 59, 1136, 1587, 1603, 2110
BmiI	GGNNCC	3 cut(s)	805, 1588, 1823
BmrFI	CCNGG	1 cut(s)	1947
BmrI	ACTGGG	3 cut(s)	96, 376, 1904
BmuI	ACTGGG	3 cut(s)	96, 376, 1904
BpiI	GAAGAC	3 cut(s)	632, 1760, 1867
BpmI	CTGGAG	2 cut(s)	1882, 2084
BsaAI	YACGTR	1 cut(s)	225
BsaJI	CCNNGG	6 cut(s)	304, 1132, 1279, 1625, 1825, 1870
BsaWI	WCCGGW	1 cut(s)	826
BsaXI	ACNNNNNCTCC	2 cut(s)	950, 980
Bsc4I	CCNNNNNNNGG	4 cut(s)	1145, 1285, 1584, 2107
Bse1I	ACTGG	7 cut(s)	91, 382, 449, 751, 856, 1899, 1910
Bse21I	CCTNAGG	1 cut(s)	1140
Bse3DI	GCAATG	2 cut(s)	553, 1319
BseAI	TCCGGA	1 cut(s)	826
BseBI	CCWGG	1 cut(s)	1947
BseDI	CCNNGG	6 cut(s)	304, 1132, 1279, 1625, 1825, 1870
BseGI	GGATG	4 cut(s)	55, 257, 1490, 2078
BseLI	CCNNNNNNNGG	4 cut(s)	1145, 1285, 1584, 2107
BseMI	GCAATG	2 cut(s)	553, 1319
BseMII	CTCAG	6 cut(s)	471, 905, 1112, 1131, 1527, 1671
BseNI	ACTGG	7 cut(s)	91, 382, 449, 751, 856, 1899, 1910
BseRI	GAGGAG	1 cut(s)	2005
BseXI	GCAGC	7 cut(s)	31, 151, 179, 1372, 1719, 1937, 2011
Bsh1236I	CGCG	2 cut(s)	325, 1361
Bsh1285I	CGRYCG	1 cut(s)	1233
BshFI	GGCC	7 cut(s)	18, 491, 684, 750, 1138, 1589, 2111
BshNI	GGYRCC	2 cut(s)	803, 1821
BsiEI	CGRYCG	1 cut(s)	1233
BsiHKAI	GWGCWC	1 cut(s)	1698
BsiHKCI	CYCGRG	1 cut(s)	1521
BsiSI	CCGG	1 cut(s)	827
BslFI	GGGAC	1 cut(s)	971
BslI	CCNNNNNNNGG	4 cut(s)	1145, 1285, 1584, 2107
BsmAI	GTCTC	1 cut(s)	970
BsmFI	GGGAC	1 cut(s)	971
BsmI	GAATGC	1 cut(s)	708
BsnI	GGCC	7 cut(s)	18, 491, 684, 750, 1138, 1589, 2111
BsoBI	CYCGRG	1 cut(s)	1521
Bsp1286I	GDGCHC	2 cut(s)	1698, 2010
Bsp13I	TCCGGA	1 cut(s)	826
Bsp1407I	TGTACA	1 cut(s)	1105
Bsp143I	GATC	5 cut(s)	228, 863, 1230, 1402, 1435
Bsp19I	CCATGG	1 cut(s)	1625
BspACI	CCGC	4 cut(s)	42, 47, 1359, 2021
BspANI	GGCC	7 cut(s)	18, 491, 684, 750, 1138, 1589, 2111
BspCNI	CTCAG	6 cut(s)	472, 906, 1113, 1132, 1528, 1672
BspEI	TCCGGA	1 cut(s)	826
BspFNI	CGCG	2 cut(s)	325, 1361
BspLI	GGNNCC	3 cut(s)	805, 1588, 1823
BspMAI	CTGCAG	2 cut(s)	169, 1390
BspMI	ACCTGC	1 cut(s)	1136
BspPI	GGATC	3 cut(s)	236, 858, 1430
BspQI	GCTCTTC	1 cut(s)	1998
BspT107I	GGYRCC	2 cut(s)	803, 1821
BsrDI	GCAATG	2 cut(s)	553, 1319
BsrGI	TGTACA	1 cut(s)	1105
BsrI	ACTGG	7 cut(s)	91, 382, 449, 751, 856, 1899, 1910
BssECI	CCNNGG	6 cut(s)	304, 1132, 1279, 1625, 1825, 1870
BssMI	GATC	5 cut(s)	228, 863, 1230, 1402, 1435
BssT1I	CCWWGG	6 cut(s)	304, 1132, 1279, 1625, 1825, 1870
Bst2UI	CCWGG	1 cut(s)	1947
Bst4CI	ACNGT	4 cut(s)	248, 776, 964, 1210
Bst6I	CTCTTC	2 cut(s)	872, 1998
BstAUI	TGTACA	1 cut(s)	1105
BstBAI	YACGTR	1 cut(s)	225
BstC8I	GCNNGC	2 cut(s)	625, 2019
BstDEI	CTNAG	9 cut(s)	70, 480, 707, 914, 1073, 1121, 1140, 1536, 1680
BstDSI	CCRYGG	1 cut(s)	1625
BstENI	CCTNNNNNAGG	1 cut(s)	1143
BstF5I	GGATG	4 cut(s)	55, 257, 1490, 2078
BstFNI	CGCG	2 cut(s)	325, 1361
BstH2I	RGCGCY	2 cut(s)	574, 769
BstHHI	GCGC	3 cut(s)	573, 768, 1363
BstKTI	GATC	5 cut(s)	231, 866, 1233, 1405, 1438
BstMAI	GTCTC	1 cut(s)	970
BstMBI	GATC	5 cut(s)	228, 863, 1230, 1402, 1435
BstMCI	CGRYCG	1 cut(s)	1233
BstMWI	GCNNNNNNNGC	7 cut(s)	173, 624, 818, 1090, 1499, 1636, 2014
BstNI	CCWGG	1 cut(s)	1947
BstNSI	RCATGY	5 cut(s)	267, 584, 1093, 1895, 2021
BstSCI	CCNGG	1 cut(s)	1945
BstSFI	CTRYAG	2 cut(s)	165, 1386
BstUI	CGCG	2 cut(s)	325, 1361
BstV1I	GCAGC	7 cut(s)	31, 151, 179, 1372, 1719, 1937, 2011
BstV2I	GAAGAC	3 cut(s)	632, 1760, 1867
BstX2I	RGATCY	1 cut(s)	863
BstXI	CCANNNNNNTGG	2 cut(s)	692, 1922
BstYI	RGATCY	1 cut(s)	863
Bsu36I	CCTNAGG	1 cut(s)	1140
BsuRI	GGCC	7 cut(s)	18, 491, 684, 750, 1138, 1589, 2111
BtgI	CCRYGG	1 cut(s)	1625
BtgZI	GCGATG	2 cut(s)	306, 633
BtrI	CACGTC	1 cut(s)	698
BtsCI	GGATG	4 cut(s)	55, 257, 1490, 2078
BtsIMutI	CAGTG	6 cut(s)	84, 442, 772, 813, 911, 960
BveI	ACCTGC	1 cut(s)	1136
Cac8I	GCNNGC	2 cut(s)	625, 2019
CaiI	CAGNNNCTG	1 cut(s)	1433
CfoI	GCGC	3 cut(s)	573, 768, 1363
Cfr13I	GGNCC	6 cut(s)	16, 59, 1136, 1587, 1603, 2110
Csp6I	GTAC	6 cut(s)	309, 327, 804, 1106, 1206, 1284
CviQI	GTAC	6 cut(s)	309, 327, 804, 1106, 1206, 1284
DdeI	CTNAG	9 cut(s)	70, 480, 707, 914, 1073, 1121, 1140, 1536, 1680
DpnI	GATC	5 cut(s)	230, 865, 1232, 1404, 1437
DpnII	GATC	5 cut(s)	228, 863, 1230, 1402, 1435
EaeI	YGGCCR	3 cut(s)	489, 682, 748
Eam1104I	CTCTTC	2 cut(s)	872, 1998
EarI	CTCTTC	2 cut(s)	872, 1998
Eco130I	CCWWGG	6 cut(s)	304, 1132, 1279, 1625, 1825, 1870
Eco24I	GRGCYC	1 cut(s)	2010
Eco32I	GATATC	1 cut(s)	486
Eco47I	GGWCC	2 cut(s)	59, 1603
Eco57I	CTGAAG	3 cut(s)	1754, 1820, 1983
Eco81I	CCTNAGG	1 cut(s)	1140
Eco88I	CYCGRG	1 cut(s)	1521
EcoNI	CCTNNNNNAGG	1 cut(s)	1143
EcoO109I	RGGNCCY	1 cut(s)	1136
EcoRI	GAATTC	1 cut(s)	1760
EcoRII	CCWGG	1 cut(s)	1945
EcoRV	GATATC	1 cut(s)	486
EcoT14I	CCWWGG	6 cut(s)	304, 1132, 1279, 1625, 1825, 1870
EcoT22I	ATGCAT	4 cut(s)	471, 823, 1782, 2080
EcoT38I	GRGCYC	1 cut(s)	2010
ErhI	CCWWGG	6 cut(s)	304, 1132, 1279, 1625, 1825, 1870
FalI	AAGNNNNNCTT	2 cut(s)	1623, 1655
FaqI	GGGAC	1 cut(s)	971
FauNDI	CATATG	1 cut(s)	1504
Fnu4HI	GCNGC	8 cut(s)	45, 165, 168, 1386, 1708, 1951, 2022, 2025
FokI	GGATG	4 cut(s)	62, 244, 1497, 2065
FriOI	GRGCYC	1 cut(s)	2010
Fsp4HI	GCNGC	8 cut(s)	45, 165, 168, 1386, 1708, 1951, 2022, 2025
FspBI	CTAG	8 cut(s)	30, 171, 305, 1280, 1400, 1857, 1865, 2048
GlaI	GCGC	3 cut(s)	572, 767, 1362
GluI	GCNGC	8 cut(s)	45, 165, 168, 1386, 1708, 1951, 2022, 2025
GsuI	CTGGAG	2 cut(s)	1882, 2084
HaeII	RGCGCY	2 cut(s)	574, 769
HaeIII	GGCC	7 cut(s)	18, 491, 684, 750, 1138, 1589, 2111
HapII	CCGG	1 cut(s)	827
HhaI	GCGC	3 cut(s)	573, 768, 1363
Hin6I	GCGC	3 cut(s)	571, 766, 1361
HinP1I	GCGC	3 cut(s)	571, 766, 1361
HincII	GTYRAC	1 cut(s)	1058
HindII	GTYRAC	1 cut(s)	1058
HinfI	GANTC	3 cut(s)	1099, 1250, 1676
HpaII	CCGG	1 cut(s)	827
HphI	GGTGA	2 cut(s)	260, 1245
Hpy166II	GTNNAC	3 cut(s)	251, 1058, 1417
Hpy188I	TCNGA	8 cut(s)	451, 587, 645, 948, 1429, 1440, 1681, 2002
Hpy188III	TCNNGA	7 cut(s)	190, 479, 827, 1044, 1223, 1234, 1535
Hpy8I	GTNNAC	3 cut(s)	251, 1058, 1417
Hpy99I	CGWCG	1 cut(s)	2042
HpyAV	CCTTC	6 cut(s)	132, 916, 1074, 1405, 1489, 1577
HpyCH4III	ACNGT	4 cut(s)	248, 776, 964, 1210
HpyCH4IV	ACGT	4 cut(s)	224, 697, 1060, 2040
HpyF10VI	GCNNNNNNNGC	7 cut(s)	173, 624, 818, 1090, 1499, 1636, 2014
HpyF3I	CTNAG	9 cut(s)	70, 480, 707, 914, 1073, 1121, 1140, 1536, 1680
HpySE526I	ACGT	4 cut(s)	224, 697, 1060, 2040
HspAI	GCGC	3 cut(s)	571, 766, 1361
Kpn2I	TCCGGA	1 cut(s)	826
KpnI	GGTACC	1 cut(s)	807
Kzo9I	GATC	5 cut(s)	228, 863, 1230, 1402, 1435
LguI	GCTCTTC	1 cut(s)	1998
LmnI	GCTCC	1 cut(s)	1814
Lsp1109I	GCAGC	7 cut(s)	31, 151, 179, 1372, 1719, 1937, 2011
MaeI	CTAG	8 cut(s)	30, 171, 305, 1280, 1400, 1857, 1865, 2048
MaeII	ACGT	4 cut(s)	224, 697, 1060, 2040
MaeIII	GTNAC	5 cut(s)	37, 82, 590, 1390, 1516
MalI	GATC	5 cut(s)	230, 865, 1232, 1404, 1437
MboI	GATC	5 cut(s)	228, 863, 1230, 1402, 1435
MflI	RGATCY	1 cut(s)	863
MhlI	GDGCHC	2 cut(s)	1698, 2010
MlsI	TGGCCA	2 cut(s)	684, 750
MluCI	AATT	9 cut(s)	978, 1039, 1154, 1243, 1508, 1565, 1689, 1760, 2028
MluNI	TGGCCA	2 cut(s)	684, 750
MmeI	TCCRAC	1 cut(s)	1822
Mox20I	TGGCCA	2 cut(s)	684, 750
Mph1103I	ATGCAT	4 cut(s)	471, 823, 1782, 2080
MroI	TCCGGA	1 cut(s)	826
MscI	TGGCCA	2 cut(s)	684, 750
MseI	TTAA	7 cut(s)	126, 159, 462, 981, 1355, 1377, 2125
MslI	CAYNNNNRTG	2 cut(s)	262, 1755
Msp20I	TGGCCA	2 cut(s)	684, 750
MspA1I	CMGCKG	1 cut(s)	44
MspI	CCGG	1 cut(s)	827
MspR9I	CCNGG	1 cut(s)	1947
Mva1269I	GAATGC	1 cut(s)	708
MvaI	CCWGG	1 cut(s)	1947
MvnI	CGCG	2 cut(s)	325, 1361
MwoI	GCNNNNNNNGC	7 cut(s)	173, 624, 818, 1090, 1499, 1636, 2014
NcoI	CCATGG	1 cut(s)	1625
NdeI	CATATG	1 cut(s)	1504
NdeII	GATC	5 cut(s)	228, 863, 1230, 1402, 1435
NlaIV	GGNNCC	3 cut(s)	805, 1588, 1823
NmeAIII	GCCGAG	1 cut(s)	2080
NmuCI	GTSAC	4 cut(s)	37, 82, 590, 1516
NsiI	ATGCAT	4 cut(s)	471, 823, 1782, 2080
NspI	RCATGY	5 cut(s)	267, 584, 1093, 1895, 2021
PaeI	GCATGC	1 cut(s)	2021
PciI	ACATGT	2 cut(s)	263, 580
PciSI	GCTCTTC	1 cut(s)	1998
PctI	GAATGC	1 cut(s)	708
PfeI	GAWTC	3 cut(s)	1099, 1250, 1676
PflFI	GACNNNGTC	1 cut(s)	962
PflMI	CCANNNNNTGG	1 cut(s)	2107
PkrI	GCNGC	8 cut(s)	46, 166, 169, 1387, 1709, 1952, 2023, 2026
Ple19I	CGATCG	1 cut(s)	1233
Ppu21I	YACGTR	1 cut(s)	225
PscI	ACATGT	2 cut(s)	263, 580
Psp6I	CCWGG	1 cut(s)	1945
PspGI	CCWGG	1 cut(s)	1945
PspN4I	GGNNCC	3 cut(s)	805, 1588, 1823
PspPI	GGNCC	6 cut(s)	16, 59, 1136, 1587, 1603, 2110
PsrI	GAACNNNNNNTAC	2 cut(s)	1920, 1952
PstI	CTGCAG	2 cut(s)	169, 1390
PstNI	CAGNNNCTG	1 cut(s)	1433
PsuI	RGATCY	1 cut(s)	863
PsyI	GACNNNGTC	1 cut(s)	962
PvuI	CGATCG	1 cut(s)	1233
RsaI	GTAC	6 cut(s)	310, 328, 805, 1107, 1207, 1285
RsaNI	GTAC	6 cut(s)	309, 327, 804, 1106, 1206, 1284
RseI	CAYNNNNRTG	2 cut(s)	262, 1755
SapI	GCTCTTC	1 cut(s)	1998
SaqAI	TTAA	7 cut(s)	126, 159, 462, 981, 1355, 1377, 2125
SatI	GCNGC	8 cut(s)	45, 165, 168, 1386, 1708, 1951, 2022, 2025
Sau3AI	GATC	5 cut(s)	228, 863, 1230, 1402, 1435
Sau96I	GGNCC	6 cut(s)	16, 59, 1136, 1587, 1603, 2110
ScrFI	CCNGG	1 cut(s)	1947
SduI	GDGCHC	2 cut(s)	1698, 2010
SfcI	CTRYAG	2 cut(s)	165, 1386
SinI	GGWCC	2 cut(s)	59, 1603
SmiMI	CAYNNNNRTG	2 cut(s)	262, 1755
SphI	GCATGC	1 cut(s)	2021
Sse9I	AATT	9 cut(s)	978, 1039, 1154, 1243, 1508, 1565, 1689, 1760, 2028
SsiI	CCGC	4 cut(s)	42, 47, 1359, 2021
SspI	AATATT	1 cut(s)	1175
SspMI	CTAG	8 cut(s)	30, 171, 305, 1280, 1400, 1857, 1865, 2048
StyD4I	CCNGG	1 cut(s)	1945
StyI	CCWWGG	6 cut(s)	304, 1132, 1279, 1625, 1825, 1870
TaaI	ACNGT	4 cut(s)	248, 776, 964, 1210
TaiI	ACGT	4 cut(s)	227, 700, 1063, 2043
TaqI	TCGA	4 cut(s)	231, 436, 534, 1233
TaqII	GACCGA	2 cut(s)	1620, 2051
TasI	AATT	9 cut(s)	978, 1039, 1154, 1243, 1508, 1565, 1689, 1760, 2028
TatI	WGTACW	1 cut(s)	1105
TauI	GCSGC	1 cut(s)	2024
TfiI	GAWTC	3 cut(s)	1099, 1250, 1676
Tru1I	TTAA	7 cut(s)	126, 159, 462, 981, 1355, 1377, 2125
Tru9I	TTAA	7 cut(s)	126, 159, 462, 981, 1355, 1377, 2125
TscAI	CASTG	6 cut(s)	91, 449, 779, 820, 918, 967
TseFI	GTSAC	4 cut(s)	37, 82, 590, 1516
TseI	GCWGC	7 cut(s)	44, 164, 167, 1385, 1707, 1950, 2024
Tsp45I	GTSAC	4 cut(s)	37, 82, 590, 1516
TspDTI	ATGAA	7 cut(s)	702, 738, 1481, 1502, 1521, 1773, 1972
TspGWI	ACGGA	2 cut(s)	51, 1986
TspRI	CASTG	6 cut(s)	91, 449, 779, 820, 918, 967
Tth111I	GACNNNGTC	1 cut(s)	962
Van91I	CCANNNNNTGG	1 cut(s)	2107
VpaK11BI	GGWCC	2 cut(s)	59, 1603
XagI	CCTNNNNNAGG	1 cut(s)	1143
XapI	RAATTY	2 cut(s)	1508, 1760
XceI	RCATGY	5 cut(s)	267, 584, 1093, 1895, 2021
XcmI	CCANNNNNNNNNTGG	3 cut(s)	779, 1597, 1632
XmaJI	CCTAGG	2 cut(s)	304, 1279
XspI	CTAG	8 cut(s)	30, 171, 305, 1280, 1400, 1857, 1865, 2048
Zsp2I	ATGCAT	4 cut(s)	471, 823, 1782, 2080

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.