Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0011022

Physical Location & Seq

Genomic Coordinates Forward (+)

35769845 .. 35776152

Gene Structure 6308 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018775

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1980 bp

ATGATGGTTGATTTAGGCATCTCACTTGCAAGATTTGCGACCAGCTCGGACAGTTATGGTCCATTTCTTCTGGGATGGCTGGTCACCGGATCATTTGGTCTTCTAGCTATCATCTTTGCATTTCTTAAATGGGAGAGAAGGACATCTCTGAATTGGGTTAAAGCTGCGGCTAGAGAAAAGAAGAAAGTCTGGAAGAAGCTAAGAGCTCCTCTGTCAACCCATACATGGATAGAAGATATTACTAATGGTGAACAGCCATCTACATGCTGTGTTTGCTTGACTTCCTTGGTGTTTCTTCAGAACCTGGGCACTAAAGCTTCATCGCGCACTCCTGTCCATCGTTGTGTAGTTTGTGGTGTGGCAGCTCATTCTTATTGTTCTGATTTTGCAGTAAAAGATTGCAAGTGTGTGGCACAAGCTGGTTCTACCCATGTTCGACACCACTGGTCAGAAAAATGGGTTAATATGGATGATAATCCTGAGATTTCTGCCTGCTGCTTCTATTGCGATGAGCCATGTGGTGTTCCTTTCCTTGATGCTTCTCCAACATGGCATTGTCTATGGTGTCAGAACCTCATACATGTCAGATGTCACAACAAAATGGCAAAAGAGTCTGGTGATGTTTGTGATCTTGGTACTCTTAGGAGGGTCATCCTTTCTCCTTTATGTGTAAAAGAAATCGGTCTCTTAAATGGAGGTGGAGTGCTGCGTTCAATAACAGATGAGTTTATAACTTCATCTGTTCGTGGCCAGTTTAGAAGAAAGCGTCATCGTTCTAAACATGGTAGTGGTCATTCAGCTAATTGTAACTCATCCAATGATGCTCTGGAGTGTCTGTTCAATGGGTTTACTGGTATGAAGAAATCCCGTAGTGAGAAGAGTTTTGACCACTTGAAGGATGGCAAGGCACTGAGCGTTAAGGGTAACCATAATGGCTTTATGCAGAAAAAGGGTGGAATCGTCACCTATGGTCAATTTAAGAAATATAAAGTGGTTAATTTACCAAAGGATGCAAGGCCTCTTTTGGTTTTTATAAATGCCAGGAGTGGAGGCCAAAATGGGGCTTCTCTAAGAAGGAGGTTGAACATGCTATTGAATCCTGCACAGGTTTTTGAATTGAGTTCTTCTCGAGGGCCTGAGGTTGGTTTGGAATTGTTCAACGATGTGCAATACTTTAGAGTTTTGGTATGTGGTGGAGATGGCACTGTTGCATGGGTCCTTGATGCCATTGAGAAGCATAAATTTGAATCGCCGCCACCTGTTGCTATTATCCCCCTTGGCACAGGAAATGATTTGTCAAGGGTTCTGCAATGGGGAAGAGGCTTCTCTATCGTTGATGGACAAGGTGGCTTAACCACGCTTTTACATGACATTAACCATGCTGCAGTTACAATGTTAGATCGCTGGAAGGTAAACATCAAATCAGAAGATGATCCAAATAAAGTGCAATCCAAGTTTATGATGAACTATTTAGGTATTGGATGTGACGCGAAGGTTGCTTATGAATTTCATGTGATGCGAGAAATAAATCCTGAGAAGTTCAGTAGTCAGTTTGTGAATAAACTGCGATATGCCAAAGAAGGTGCTAGAGATATAATGGACCGAACTTGTGCCGACTTGCCATGGCAAGTTTGGCTTGAAGTTGATGGGAAAGACATAGATATTCCAAAGGATTCTGAGGGTCTAATCGTGCTCAACATTGGCAGCTACATGGGTGGAGTGGATCTTTGGCAAAATGATATTGATCATGATGATGACTTTAGTCTTCAGTCAATGCATGATAAAATGCTTGAAGTTGTATGCATATGTGGATCGTGGCACCTTGGCAAACTACAGGTTGGACTATCACAAGCTAGGAGACTAGCCCAGGGGAATATCATAAGAATACATGCATCTAGTCCTTTTCCAGTCCAAATAGATGGGGAGCCATATATACAACAACCTGGCTGTATAGAGATAACACATCATGGGCAGGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004143 GO:0006793 GO:0006796 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009611 GO:0009628 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0022622 GO:0032501 GO:0032502 GO:0044237 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

C1_1 DAGK_acc DAGK_cat

Protein Analysis

660

Amino Acids

73.4

Weight (kDa)

8.53

Isoelectric Point (pI)

46.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C1_1	PF00130	147 - 206	1e-06	Phorbol esters/diacylglycerol binding domain (C1 domain)
DAGK_cat	PF00781	340 - 440	2e-26	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	486 - 643	1e-57	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011022)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63770 AT5G63770
fragaria_vesca	FvH4_1g25600 FvH4_1g25600 FvH4_1g25600
malus_domestica	MD15G1139400.v1.1
prunus_persica	Prupe.4G282200_v2.0.a1 Prupe.4G282200_v2.0.a1 Prupe.4G282200_v2.0.a1
pyrus_communis	pycom15g12580
rosa_chinensis	RchiOBHm_Chr2g0123771
rosa_laevigata	RLG00000018775
rosa_multiflora	Rmu_sc0001032.1_g000003
rosa_roxburghii	Rroxscaffold_2G00120350
rosa_rugosa	Rorug02G0247900
rosa_samantha	Rh2AG306800 Rh2BG315300 Rh2CG293200 Rh2DG330500
rosa_wichuraiana	Rw2G024620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	731, 1034
Acc36I	ACCTGC	1 cut(s)	1963
AccB1I	GGYRCC	1 cut(s)	1818
AccII	CGCG	2 cut(s)	325, 1490
AciI	CCGC	2 cut(s)	167, 1253
AclWI	GGATC	4 cut(s)	97, 1427, 1731, 1819
AcoI	YGGCCR	1 cut(s)	748
AcsI	RAATTY	2 cut(s)	1241, 1505
AcuI	CTGAAG	2 cut(s)	281, 1751
AfaI	GTAC	1 cut(s)	637
AfiI	CCNNNNNNNGG	4 cut(s)	225, 895, 1060, 1142
AflIII	ACRYGT	1 cut(s)	580
AjnI	CCWGG	4 cut(s)	303, 1040, 1866, 1942
AloI	GAACNNNNNNTCC	2 cut(s)	821, 853
Alw21I	GWGCWC	2 cut(s)	208, 1695
Alw26I	GTCTC	2 cut(s)	689, 1852
AlwI	GGATC	4 cut(s)	97, 1427, 1731, 1819
AlwNI	CAGNNNCTG	1 cut(s)	304
Ama87I	CYCGRG	1 cut(s)	1128
AoxI	GGCC	4 cut(s)	748, 1016, 1051, 1133
ApeKI	GCWGC	6 cut(s)	164, 362, 495, 706, 1382, 1704
ApoI	RAATTY	2 cut(s)	1241, 1505
AspLEI	GCGC	1 cut(s)	327
AspS9I	GGNCC	4 cut(s)	59, 1133, 1216, 1600
AsuHPI	GGTGA	4 cut(s)	76, 260, 629, 955
AvaI	CYCGRG	1 cut(s)	1128
AvaII	GGWCC	3 cut(s)	59, 1216, 1600
AxyI	CCTNAGG	1 cut(s)	1137
BaeGI	GKGCMC	1 cut(s)	311
BalI	TGGCCA	1 cut(s)	750
BanI	GGYRCC	1 cut(s)	1818
BanII	GRGCYC	1 cut(s)	208
BbsI	GAAGAC	2 cut(s)	92, 1757
Bbv12I	GWGCWC	2 cut(s)	208, 1695
BbvI	GCAGC	6 cut(s)	151, 374, 482, 693, 1369, 1716
BccI	CCATC	8 cut(s)	69, 265, 345, 893, 1193, 1331, 1640, 1913
BciT130I	CCWGG	4 cut(s)	305, 1042, 1868, 1944
BclI	TGATCA	1 cut(s)	1744
BcoDI	GTCTC	2 cut(s)	689, 1852
BfaI	CTAG	6 cut(s)	104, 171, 1587, 1854, 1862, 1896
BfmI	CTRYAG	2 cut(s)	1383, 1832
BfuAI	ACCTGC	1 cut(s)	1963
BisI	GCNGC	8 cut(s)	165, 168, 363, 496, 707, 1253, 1383, 1705
BlsI	GCNGC	8 cut(s)	166, 169, 364, 497, 708, 1254, 1384, 1706
Bme1390I	CCNGG	4 cut(s)	305, 1042, 1868, 1944
Bme18I	GGWCC	3 cut(s)	59, 1216, 1600
BmeT110I	CYCGRG	1 cut(s)	1128
BmgT120I	GGNCC	4 cut(s)	59, 1133, 1216, 1600
BmiI	GGNNCC	3 cut(s)	1217, 1820, 1926
BmrFI	CCNGG	4 cut(s)	305, 1042, 1868, 1944
BmsI	GCATC	7 cut(s)	27, 526, 811, 1000, 1213, 1506, 1901
BoxI	GACNNNNGTC	1 cut(s)	1761
BpiI	GAAGAC	2 cut(s)	92, 1757
BplI	GAGNNNNNCTC	2 cut(s)	1111, 1143
BpmI	CTGGAG	1 cut(s)	848
BsaBI	GATNNNNATC	2 cut(s)	474, 1743
BsaI	GGTCTC	1 cut(s)	689
BsaJI	CCNNGG	7 cut(s)	285, 304, 1276, 1622, 1822, 1866, 1867
BsaWI	WCCGGW	1 cut(s)	86
BsaXI	ACNNNNNCTCC	4 cut(s)	687, 717, 821, 851
Bsc4I	CCNNNNNNNGG	4 cut(s)	225, 895, 1060, 1142
Bse1I	ACTGG	4 cut(s)	449, 751, 856, 1907
Bse21I	CCTNAGG	1 cut(s)	1137
Bse3DI	GCAATG	1 cut(s)	1316
Bse8I	GATNNNNATC	2 cut(s)	474, 1743
BseBI	CCWGG	4 cut(s)	305, 1042, 1868, 1944
BseDI	CCNNGG	7 cut(s)	285, 304, 1276, 1622, 1822, 1866, 1867
BseGI	GGATG	7 cut(s)	80, 475, 651, 812, 904, 1015, 1487
BseJI	GATNNNNATC	2 cut(s)	474, 1743
BseLI	CCNNNNNNNGG	4 cut(s)	225, 895, 1060, 1142
BseMI	GCAATG	1 cut(s)	1316
BseMII	CTCAG	5 cut(s)	471, 902, 1128, 1524, 1668
BseNI	ACTGG	4 cut(s)	449, 751, 856, 1907
BseRI	GAGGAG	1 cut(s)	198
BseSI	GKGCMC	1 cut(s)	311
BseXI	GCAGC	6 cut(s)	151, 374, 482, 693, 1369, 1716
BsgI	GTGCAG	1 cut(s)	1086
Bsh1236I	CGCG	2 cut(s)	325, 1490
BshFI	GGCC	4 cut(s)	750, 1018, 1053, 1135
BshNI	GGYRCC	1 cut(s)	1818
BsiHKAI	GWGCWC	2 cut(s)	208, 1695
BsiHKCI	CYCGRG	1 cut(s)	1128
BsiSI	CCGG	1 cut(s)	87
BslI	CCNNNNNNNGG	4 cut(s)	225, 895, 1060, 1142
BsmAI	GTCTC	2 cut(s)	689, 1852
BsnI	GGCC	4 cut(s)	750, 1018, 1053, 1135
Bso31I	GGTCTC	1 cut(s)	689
BsoBI	CYCGRG	1 cut(s)	1128
Bsp1286I	GDGCHC	3 cut(s)	208, 311, 1695
Bsp143I	GATC	7 cut(s)	89, 628, 1399, 1432, 1723, 1744, 1811
Bsp19I	CCATGG	1 cut(s)	1622
BspACI	CCGC	2 cut(s)	167, 1253
BspANI	GGCC	4 cut(s)	750, 1018, 1053, 1135
BspCNI	CTCAG	5 cut(s)	472, 903, 1129, 1525, 1669
BspFNI	CGCG	2 cut(s)	325, 1490
BspHI	TCATGA	1 cut(s)	1747
BspLI	GGNNCC	3 cut(s)	1217, 1820, 1926
BspMAI	CTGCAG	1 cut(s)	1387
BspMI	ACCTGC	1 cut(s)	1963
BspPI	GGATC	4 cut(s)	97, 1427, 1731, 1819
BspT107I	GGYRCC	1 cut(s)	1818
BspTNI	GGTCTC	1 cut(s)	689
BsrDI	GCAATG	1 cut(s)	1316
BsrI	ACTGG	4 cut(s)	449, 751, 856, 1907
BssECI	CCNNGG	7 cut(s)	285, 304, 1276, 1622, 1822, 1866, 1867
BssMI	GATC	7 cut(s)	89, 628, 1399, 1432, 1723, 1744, 1811
BssT1I	CCWWGG	4 cut(s)	285, 1276, 1622, 1822
Bst2UI	CCWGG	4 cut(s)	305, 1042, 1868, 1944
Bst4CI	ACNGT	2 cut(s)	53, 1207
Bst6I	CTCTTC	2 cut(s)	872, 1312
BstAPI	GCANNNNNTGC	1 cut(s)	35
BstC8I	GCNNGC	1 cut(s)	493
BstDEI	CTNAG	8 cut(s)	200, 480, 641, 911, 1070, 1137, 1533, 1677
BstDSI	CCRYGG	1 cut(s)	1622
BstEII	GGTNACC	2 cut(s)	82, 923
BstF5I	GGATG	7 cut(s)	80, 475, 651, 812, 904, 1015, 1487
BstFNI	CGCG	2 cut(s)	325, 1490
BstHHI	GCGC	1 cut(s)	327
BstKTI	GATC	7 cut(s)	92, 631, 1402, 1435, 1726, 1747, 1814
BstMAI	GTCTC	2 cut(s)	689, 1852
BstMBI	GATC	7 cut(s)	89, 628, 1399, 1432, 1723, 1744, 1811
BstMWI	GCNNNNNNNGC	5 cut(s)	35, 273, 504, 1496, 1633
BstNI	CCWGG	4 cut(s)	305, 1042, 1868, 1944
BstNSI	RCATGY	4 cut(s)	267, 584, 1090, 1892
BstPAI	GACNNNNGTC	1 cut(s)	1761
BstPI	GGTNACC	2 cut(s)	82, 923
BstSCI	CCNGG	4 cut(s)	303, 1040, 1866, 1942
BstSFI	CTRYAG	2 cut(s)	1383, 1832
BstSLI	GKGCMC	1 cut(s)	311
BstUI	CGCG	2 cut(s)	325, 1490
BstV1I	GCAGC	6 cut(s)	151, 374, 482, 693, 1369, 1716
BstV2I	GAAGAC	2 cut(s)	92, 1757
BstX2I	RGATCY	1 cut(s)	1723
BstXI	CCANNNNNNTGG	1 cut(s)	1919
BstYI	RGATCY	1 cut(s)	1723
Bsu36I	CCTNAGG	1 cut(s)	1137
BsuRI	GGCC	4 cut(s)	750, 1018, 1053, 1135
BtgI	CCRYGG	1 cut(s)	1622
BtgZI	GCGATG	2 cut(s)	306, 522
BtsCI	GGATG	7 cut(s)	80, 475, 651, 812, 904, 1015, 1487
BtsIMutI	CAGTG	3 cut(s)	442, 908, 1203
BveI	ACCTGC	1 cut(s)	1963
Cac8I	GCNNGC	1 cut(s)	493
CaiI	CAGNNNCTG	1 cut(s)	304
CciI	TCATGA	1 cut(s)	1747
CfoI	GCGC	1 cut(s)	327
Cfr13I	GGNCC	4 cut(s)	59, 1133, 1216, 1600
CseI	GACGC	2 cut(s)	755, 1496
Csp6I	GTAC	1 cut(s)	636
CviQI	GTAC	1 cut(s)	636
DdeI	CTNAG	8 cut(s)	200, 480, 641, 911, 1070, 1137, 1533, 1677
DpnI	GATC	7 cut(s)	91, 630, 1401, 1434, 1725, 1746, 1813
DpnII	GATC	7 cut(s)	89, 628, 1399, 1432, 1723, 1744, 1811
EaeI	YGGCCR	1 cut(s)	748
Eam1104I	CTCTTC	2 cut(s)	872, 1312
EarI	CTCTTC	2 cut(s)	872, 1312
Ecl136II	GAGCTC	1 cut(s)	206
Eco130I	CCWWGG	4 cut(s)	285, 1276, 1622, 1822
Eco147I	AGGCCT	1 cut(s)	1018
Eco24I	GRGCYC	1 cut(s)	208
Eco31I	GGTCTC	1 cut(s)	689
Eco47I	GGWCC	3 cut(s)	59, 1216, 1600
Eco53kI	GAGCTC	1 cut(s)	206
Eco57I	CTGAAG	2 cut(s)	281, 1751
Eco81I	CCTNAGG	1 cut(s)	1137
Eco88I	CYCGRG	1 cut(s)	1128
Eco91I	GGTNACC	2 cut(s)	82, 923
EcoICRI	GAGCTC	1 cut(s)	206
EcoO109I	RGGNCCY	2 cut(s)	1133, 1216
EcoO65I	GGTNACC	2 cut(s)	82, 923
EcoRII	CCWGG	4 cut(s)	303, 1040, 1866, 1942
EcoT14I	CCWWGG	4 cut(s)	285, 1276, 1622, 1822
EcoT22I	ATGCAT	3 cut(s)	1779, 1805, 1894
EcoT38I	GRGCYC	1 cut(s)	208
ErhI	CCWWGG	4 cut(s)	285, 1276, 1622, 1822
FalI	AAGNNNNNCTT	2 cut(s)	1620, 1652
FauNDI	CATATG	1 cut(s)	1805
FbaI	TGATCA	1 cut(s)	1744
Fnu4HI	GCNGC	8 cut(s)	165, 168, 363, 496, 707, 1253, 1383, 1705
FokI	GGATG	7 cut(s)	87, 482, 638, 799, 911, 1022, 1494
FriOI	GRGCYC	1 cut(s)	208
Fsp4HI	GCNGC	8 cut(s)	165, 168, 363, 496, 707, 1253, 1383, 1705
FspBI	CTAG	6 cut(s)	104, 171, 1587, 1854, 1862, 1896
GlaI	GCGC	1 cut(s)	326
GluI	GCNGC	8 cut(s)	165, 168, 363, 496, 707, 1253, 1383, 1705
GsuI	CTGGAG	1 cut(s)	848
HaeIII	GGCC	4 cut(s)	750, 1018, 1053, 1135
HapII	CCGG	1 cut(s)	87
HgaI	GACGC	2 cut(s)	755, 1496
HhaI	GCGC	1 cut(s)	327
Hin6I	GCGC	1 cut(s)	325
HinP1I	GCGC	1 cut(s)	325
HincII	GTYRAC	1 cut(s)	216
HindII	GTYRAC	1 cut(s)	216
HindIII	AAGCTT	1 cut(s)	315
HinfI	GANTC	5 cut(s)	611, 957, 1096, 1247, 1673
HpaII	CCGG	1 cut(s)	87
HphI	GGTGA	4 cut(s)	76, 260, 629, 955
Hpy166II	GTNNAC	4 cut(s)	216, 251, 849, 1414
Hpy188I	TCNGA	9 cut(s)	49, 150, 300, 382, 451, 570, 587, 1426, 1678
Hpy188III	TCNNGA	6 cut(s)	190, 479, 827, 1128, 1532, 1748
Hpy8I	GTNNAC	4 cut(s)	216, 251, 849, 1414
HpyAV	CCTTC	6 cut(s)	132, 889, 1068, 1402, 1486, 1574
HpyCH4III	ACNGT	2 cut(s)	53, 1207
HpyF10VI	GCNNNNNNNGC	5 cut(s)	35, 273, 504, 1496, 1633
HpyF3I	CTNAG	8 cut(s)	200, 480, 641, 911, 1070, 1137, 1533, 1677
HspAI	GCGC	1 cut(s)	325
Ksp22I	TGATCA	1 cut(s)	1744
Kzo9I	GATC	7 cut(s)	89, 628, 1399, 1432, 1723, 1744, 1811
LmnI	GCTCC	2 cut(s)	211, 1924
Lsp1109I	GCAGC	6 cut(s)	151, 374, 482, 693, 1369, 1716
LweI	GCATC	7 cut(s)	27, 526, 811, 1000, 1213, 1506, 1901
MaeI	CTAG	6 cut(s)	104, 171, 1587, 1854, 1862, 1896
MaeIII	GTNAC	7 cut(s)	82, 590, 806, 923, 961, 1387, 1484
MalI	GATC	7 cut(s)	91, 630, 1401, 1434, 1725, 1746, 1813
MboI	GATC	7 cut(s)	89, 628, 1399, 1432, 1723, 1744, 1811
MflI	RGATCY	1 cut(s)	1723
MhlI	GDGCHC	3 cut(s)	208, 311, 1695
MlsI	TGGCCA	1 cut(s)	750
MluCI	AATT	8 cut(s)	151, 802, 974, 997, 1115, 1151, 1241, 1505
MluNI	TGGCCA	1 cut(s)	750
MlyI	GAGTC	1 cut(s)	620
MmeI	TCCRAC	2 cut(s)	569, 1819
Mox20I	TGGCCA	1 cut(s)	750
Mph1103I	ATGCAT	3 cut(s)	1779, 1805, 1894
MscI	TGGCCA	1 cut(s)	750
MseI	TTAA	9 cut(s)	126, 159, 462, 689, 918, 978, 996, 1352, 1374
MslI	CAYNNNNRTG	4 cut(s)	262, 342, 786, 1752
Msp20I	TGGCCA	1 cut(s)	750
MspI	CCGG	1 cut(s)	87
MspR9I	CCNGG	4 cut(s)	305, 1042, 1868, 1944
MvaI	CCWGG	4 cut(s)	305, 1042, 1868, 1944
MvnI	CGCG	2 cut(s)	325, 1490
MwoI	GCNNNNNNNGC	5 cut(s)	35, 273, 504, 1496, 1633
NcoI	CCATGG	1 cut(s)	1622
NdeI	CATATG	1 cut(s)	1805
NdeII	GATC	7 cut(s)	89, 628, 1399, 1432, 1723, 1744, 1811
NlaIV	GGNNCC	3 cut(s)	1217, 1820, 1926
NmuCI	GTSAC	4 cut(s)	82, 590, 961, 1484
NsiI	ATGCAT	3 cut(s)	1779, 1805, 1894
NspI	RCATGY	4 cut(s)	267, 584, 1090, 1892
PaeR7I	CTCGAG	1 cut(s)	1128
PagI	TCATGA	1 cut(s)	1747
PasI	CCCWGGG	1 cut(s)	1867
PceI	AGGCCT	1 cut(s)	1018
PciI	ACATGT	1 cut(s)	580
PfeI	GAWTC	4 cut(s)	957, 1096, 1247, 1673
PkrI	GCNGC	8 cut(s)	166, 169, 364, 497, 708, 1254, 1384, 1706
PleI	GAGTC	1 cut(s)	619
PpsI	GAGTC	1 cut(s)	619
PpuMI	RGGWCCY	1 cut(s)	1216
PscI	ACATGT	1 cut(s)	580
PshAI	GACNNNNGTC	1 cut(s)	1761
PsiI	TTATAA	2 cut(s)	731, 1034
Psp124BI	GAGCTC	1 cut(s)	208
Psp5II	RGGWCCY	1 cut(s)	1216
Psp6I	CCWGG	4 cut(s)	303, 1040, 1866, 1942
PspEI	GGTNACC	2 cut(s)	82, 923
PspGI	CCWGG	4 cut(s)	303, 1040, 1866, 1942
PspN4I	GGNNCC	3 cut(s)	1217, 1820, 1926
PspPI	GGNCC	4 cut(s)	59, 1133, 1216, 1600
PspPPI	RGGWCCY	1 cut(s)	1216
PstI	CTGCAG	1 cut(s)	1387
PstNI	CAGNNNCTG	1 cut(s)	304
PsuI	RGATCY	1 cut(s)	1723
RsaI	GTAC	1 cut(s)	637
RsaNI	GTAC	1 cut(s)	636
RseI	CAYNNNNRTG	4 cut(s)	262, 342, 786, 1752
SacI	GAGCTC	1 cut(s)	208
SaqAI	TTAA	9 cut(s)	126, 159, 462, 689, 918, 978, 996, 1352, 1374
SatI	GCNGC	8 cut(s)	165, 168, 363, 496, 707, 1253, 1383, 1705
Sau3AI	GATC	7 cut(s)	89, 628, 1399, 1432, 1723, 1744, 1811
Sau96I	GGNCC	4 cut(s)	59, 1133, 1216, 1600
SchI	GAGTC	1 cut(s)	620
ScrFI	CCNGG	4 cut(s)	305, 1042, 1868, 1944
SduI	GDGCHC	3 cut(s)	208, 311, 1695
SfaNI	GCATC	7 cut(s)	27, 526, 811, 1000, 1213, 1506, 1901
SfcI	CTRYAG	2 cut(s)	1383, 1832
Sfr274I	CTCGAG	1 cut(s)	1128
SinI	GGWCC	3 cut(s)	59, 1216, 1600
SlaI	CTCGAG	1 cut(s)	1128
SmiMI	CAYNNNNRTG	4 cut(s)	262, 342, 786, 1752
SmlI	CTYRAG	1 cut(s)	1128
SmoI	CTYRAG	1 cut(s)	1128
Sse9I	AATT	8 cut(s)	151, 802, 974, 997, 1115, 1151, 1241, 1505
SseBI	AGGCCT	1 cut(s)	1018
SsiI	CCGC	2 cut(s)	167, 1253
SspMI	CTAG	6 cut(s)	104, 171, 1587, 1854, 1862, 1896
SstI	GAGCTC	1 cut(s)	208
StuI	AGGCCT	1 cut(s)	1018
StyD4I	CCNGG	4 cut(s)	303, 1040, 1866, 1942
StyI	CCWWGG	4 cut(s)	285, 1276, 1622, 1822
TaaI	ACNGT	2 cut(s)	53, 1207
TaqI	TCGA	2 cut(s)	436, 1129
TaqII	GACCGA	2 cut(s)	671, 1617
TasI	AATT	8 cut(s)	151, 802, 974, 997, 1115, 1151, 1241, 1505
TauI	GCSGC	2 cut(s)	170, 1255
TfiI	GAWTC	4 cut(s)	957, 1096, 1247, 1673
Tru1I	TTAA	9 cut(s)	126, 159, 462, 689, 918, 978, 996, 1352, 1374
Tru9I	TTAA	9 cut(s)	126, 159, 462, 689, 918, 978, 996, 1352, 1374
TscAI	CASTG	3 cut(s)	449, 915, 1210
TseFI	GTSAC	4 cut(s)	82, 590, 961, 1484
TseI	GCWGC	6 cut(s)	164, 362, 495, 706, 1382, 1704
Tsp45I	GTSAC	4 cut(s)	82, 590, 961, 1484
TspDTI	ATGAA	6 cut(s)	309, 726, 872, 1478, 1499, 1518
TspRI	CASTG	3 cut(s)	449, 915, 1210
VpaK11BI	GGWCC	3 cut(s)	59, 1216, 1600
XapI	RAATTY	2 cut(s)	1241, 1505
XceI	RCATGY	4 cut(s)	267, 584, 1090, 1892
XcmI	CCANNNNNNNNNTGG	3 cut(s)	823, 896, 1629
XhoI	CTCGAG	1 cut(s)	1128
XspI	CTAG	6 cut(s)	104, 171, 1587, 1854, 1862, 1896
Zsp2I	ATGCAT	3 cut(s)	1779, 1805, 1894

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.