Rh2CG293200

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0011022
Physical Location & Seq
Forward (+)
33256414 .. 33262876
6463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG293200.1

Sequence Viewer

Length: 1596 bp
ATGATGGTCGATTTAGGCATCTCACTTGCAAGATTTGTGACCAGCTCGGACAGTTATGGTCCATTTCTCCTGGGATGGCTGGTCACCGGATCATTTGGTCTTCTAGCTATCATCTTTGCATTTCTTAAATGGGAGAGAAGGACATCTCTGAATTGGGTTAAAGCTGCGGCTAGAGAAAAGAAGAAAGTCTGGAAGAAGCTAAGAGCTCCTCTGTCAACCCATACATGGATAGAAGATATTACTAATGGTGAACAGCCATCTACATGCTGTGTTTGCTTGACTTCCTTGGTGTTTCTTCAGAACATAAGCACTAAAGCTTCATTGCGCACTTCTGTCCATCGTTGTGTAGTTTGTGGTGTGGCAGCTCATTCTTATTGTTCTGATTTTGCAGTAAAAGATTGCAAGTGTGTGGCACAAGCTGGTTCTACCCATGTTCGACACCACTGGTCAGAAAAATGGGTTAATATGGATGATAATCCTGAGATTTCTGCCTGCTGCTTCTATTGCGATGAGCCATGTGGTGTTCCTTTCCTTGATGCTTCTCCAACATGGCATTGTCTATGGTGTCAGAACCTCATACATGTCAGATGTCACAATAAAATGGCAAAAGACTCTGGTGATGTTTGTGATCTTGGTACTCTTAGGAGGGTCATCCTTTCTCCTTTATGTGTAAAAGAAATCGGTTACTTAAATGGAGGTGGAGTGCTGCGTTCAATAACAGATGAGTTTATAACTTCATCTGTTCGTGGCCAGTTTAGAAGAAAGCGTCATCGTTCTAAACATGGTAGTGGTCATTCAGCTAATTGTAACTCATCCAATGATGCTCTGGAGTATCTGTTCAATGGGTTTACTGGTATGAAGAAATCCCGTAGTGAGAGGAGTTTTGACCACTTGAAGGATGGCAGGGCACTGAGTGTTAAGGGTAACCATAATGGCTTTATGCAGAAAAAGGGTGGAATTGTCACCTATGGTCAATTTAAGAAATATAAAGTGGTTAATTTACCAAAGGATGCAAGGCCTCTTTTGGTTTTTATAAATGCCAGGAGTGGAGGCCAAAACGGGGCTTCTCTAAGAAGGAGGTTGAACATGCTATTGAATCCTGCACAGGTTTTTGAATTGAGTTCTTCTCAAGGGCCTGAGGTTGGTTTGGAATTGTTCAACGATGTGCAATACTTTAGAGTTTTGGTATGTGGTGGAGATGGCACTGTTGCATGGGTCCTTGATGCCATTGAGAAGCATAATTTTGAATCACCGCCACCTGTTGCTATTATCCCCCTTGGCACAGGAAATGATTTGTCAAGGGTTCTGCAATGGGGAAGAGGCTTCTCTATCGTTGATGGACAAGGTGGCTTAACCACGCTTTTACATGACATTAACCATGCTGCAGTTACAATGTTAGATCGCTGGAAGGTAAACATCAAATCAGAAGATGATCCAAATAAAGTGCAATCCAAGTTTATGATGAACTATTTAGGTATTGGATGTGACGCGAAGGTTGCTTATGAATTTCATGTGATGCGAGAAATAAATCCTGAGAAGTTCAGTAGTCAGGTACCTGCATTTATATTCGGGTGGCACTGTCTTATTGTTTTATAA

Protein Analysis

531

Amino Acids

59.22

Weight (kDa)

9.03

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C1_1 PF00130 147 - 206 1.3e-06 Phorbol esters/diacylglycerol binding domain (C1 domain)
DAGK_cat PF00781 340 - 440 6.6e-27 Diacylglycerol kinase catalytic domain
DAGK_acc PF00609 486 - 518 1.5e-09 Diacylglycerol kinase accessory domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011022)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63770 AT5G63770
fragaria_vesca FvH4_1g25600 FvH4_1g25600 FvH4_1g25600
malus_domestica MD15G1139400.v1.1
prunus_persica Prupe.4G282200_v2.0.a1 Prupe.4G282200_v2.0.a1 Prupe.4G282200_v2.0.a1
pyrus_communis pycom15g12580
rosa_chinensis RchiOBHm_Chr2g0123771
rosa_laevigata RLG00000018775
rosa_multiflora Rmu_sc0001032.1_g000003
rosa_roxburghii Rroxscaffold_2G00120350
rosa_rugosa Rorug02G0247900
rosa_samantha Rh2AG306800 Rh2BG315300 Rh2CG293200 Rh2DG330500
rosa_wichuraiana Rw2G024620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 731, 1034, 1594
Acc16I TGCGCA 1 cut(s) 326
Acc36I ACCTGC 1 cut(s) 1564
Acc65I GGTACC 1 cut(s) 1552
AccB1I GGYRCC 1 cut(s) 1552
AccII CGCG 1 cut(s) 1490
AciI CCGC 2 cut(s) 167, 1253
AclWI GGATC 2 cut(s) 97, 1427
AcoI YGGCCR 1 cut(s) 748
AcsI RAATTY 1 cut(s) 1505
AcuI CTGAAG 1 cut(s) 281
AdeI CACNNNGTG 1 cut(s) 914
AfaI GTAC 2 cut(s) 637, 1554
AfiI CCNNNNNNNGG 4 cut(s) 225, 895, 1060, 1142
AflIII ACRYGT 1 cut(s) 580
AgsI TTSAA 8 cut(s) 714, 841, 895, 1084, 1096, 1115, 1159, 1247
AjnI CCWGG 2 cut(s) 69, 1040
AloI GAACNNNNNNTCC 2 cut(s) 821, 853
AluBI AGCT 9 cut(s) 45, 107, 164, 199, 206, 317, 365, 419, 800
AluI AGCT 9 cut(s) 45, 107, 164, 199, 206, 317, 365, 419, 800
Alw21I GWGCWC 1 cut(s) 208
AlwI GGATC 2 cut(s) 97, 1427
AoxI GGCC 4 cut(s) 748, 1016, 1051, 1133
ApeKI GCWGC 5 cut(s) 164, 362, 495, 706, 1382
ApoI RAATTY 1 cut(s) 1505
Asp718I GGTACC 1 cut(s) 1552
AspLEI GCGC 1 cut(s) 327
AspS9I GGNCC 3 cut(s) 59, 1133, 1216
AsuHPI GGTGA 5 cut(s) 76, 260, 629, 955, 1242
AvaII GGWCC 2 cut(s) 59, 1216
AxyI CCTNAGG 1 cut(s) 1137
BaeGI GKGCMC 1 cut(s) 910
BalI TGGCCA 1 cut(s) 750
BanI GGYRCC 1 cut(s) 1552
BanII GRGCYC 1 cut(s) 208
BbsI GAAGAC 1 cut(s) 92
Bbv12I GWGCWC 1 cut(s) 208
BbvI GCAGC 5 cut(s) 151, 374, 482, 693, 1369
BccI CCATC 6 cut(s) 69, 265, 345, 893, 1193, 1331
BciT130I CCWGG 2 cut(s) 71, 1042
BfaI CTAG 2 cut(s) 104, 171
BfmI CTRYAG 1 cut(s) 1383
BfuAI ACCTGC 1 cut(s) 1564
BisI GCNGC 6 cut(s) 165, 168, 363, 496, 707, 1383
BlsI GCNGC 6 cut(s) 166, 169, 364, 497, 708, 1384
Bme1390I CCNGG 2 cut(s) 71, 1042
Bme18I GGWCC 2 cut(s) 59, 1216
BmgT120I GGNCC 3 cut(s) 59, 1133, 1216
BmiI GGNNCC 2 cut(s) 1217, 1554
BmrFI CCNGG 2 cut(s) 71, 1042
BmsI GCATC 6 cut(s) 27, 526, 811, 1000, 1213, 1506
BpiI GAAGAC 1 cut(s) 92
BplI GAGNNNNNCTC 2 cut(s) 1111, 1143
BpmI CTGGAG 1 cut(s) 848
BpuEI CTTGAG 1 cut(s) 1113
BsaBI GATNNNNATC 1 cut(s) 474
BsaJI CCNNGG 3 cut(s) 70, 285, 1276
BsaWI WCCGGW 1 cut(s) 86
BsaXI ACNNNNNCTCC 4 cut(s) 687, 717, 821, 851
Bsc4I CCNNNNNNNGG 4 cut(s) 225, 895, 1060, 1142
Bse1I ACTGG 3 cut(s) 449, 751, 856
Bse21I CCTNAGG 1 cut(s) 1137
Bse3DI GCAATG 2 cut(s) 320, 1316
Bse8I GATNNNNATC 1 cut(s) 474
BseBI CCWGG 2 cut(s) 71, 1042
BseDI CCNNGG 3 cut(s) 70, 285, 1276
BseGI GGATG 7 cut(s) 80, 475, 651, 812, 904, 1015, 1487
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 4 cut(s) 225, 895, 1060, 1142
BseMI GCAATG 2 cut(s) 320, 1316
BseMII CTCAG 4 cut(s) 471, 902, 1128, 1524
BseNI ACTGG 3 cut(s) 449, 751, 856
BseRI GAGGAG 2 cut(s) 198, 892
BseSI GKGCMC 1 cut(s) 910
BseXI GCAGC 5 cut(s) 151, 374, 482, 693, 1369
BsgI GTGCAG 1 cut(s) 1086
Bsh1236I CGCG 1 cut(s) 1490
BshFI GGCC 4 cut(s) 750, 1018, 1053, 1135
BshNI GGYRCC 1 cut(s) 1552
BsiHKAI GWGCWC 1 cut(s) 208
BsiSI CCGG 1 cut(s) 87
BslI CCNNNNNNNGG 4 cut(s) 225, 895, 1060, 1142
BsnI GGCC 4 cut(s) 750, 1018, 1053, 1135
Bsp1286I GDGCHC 2 cut(s) 208, 910
Bsp143I GATC 4 cut(s) 89, 628, 1399, 1432
BspACI CCGC 2 cut(s) 167, 1253
BspANI GGCC 4 cut(s) 750, 1018, 1053, 1135
BspCNI CTCAG 4 cut(s) 472, 903, 1129, 1525
BspFNI CGCG 1 cut(s) 1490
BspLI GGNNCC 2 cut(s) 1217, 1554
BspMAI CTGCAG 1 cut(s) 1387
BspMI ACCTGC 1 cut(s) 1564
BspPI GGATC 2 cut(s) 97, 1427
BspT107I GGYRCC 1 cut(s) 1552
BsrDI GCAATG 2 cut(s) 320, 1316
BsrI ACTGG 3 cut(s) 449, 751, 856
BssECI CCNNGG 3 cut(s) 70, 285, 1276
BssMI GATC 4 cut(s) 89, 628, 1399, 1432
BssT1I CCWWGG 2 cut(s) 285, 1276
Bst2UI CCWGG 2 cut(s) 71, 1042
Bst4CI ACNGT 3 cut(s) 53, 1207, 1580
Bst6I CTCTTC 1 cut(s) 1312
BstC8I GCNNGC 1 cut(s) 493
BstDEI CTNAG 7 cut(s) 200, 480, 641, 911, 1070, 1137, 1533
BstEII GGTNACC 2 cut(s) 82, 923
BstF5I GGATG 7 cut(s) 80, 475, 651, 812, 904, 1015, 1487
BstFNI CGCG 1 cut(s) 1490
BstHHI GCGC 1 cut(s) 327
BstKTI GATC 4 cut(s) 92, 631, 1402, 1435
BstMBI GATC 4 cut(s) 89, 628, 1399, 1432
BstMWI GCNNNNNNNGC 3 cut(s) 273, 504, 1496
BstNI CCWGG 2 cut(s) 71, 1042
BstNSI RCATGY 3 cut(s) 267, 584, 1090
BstPI GGTNACC 2 cut(s) 82, 923
BstSCI CCNGG 2 cut(s) 69, 1040
BstSFI CTRYAG 1 cut(s) 1383
BstSLI GKGCMC 1 cut(s) 910
BstUI CGCG 1 cut(s) 1490
BstV1I GCAGC 5 cut(s) 151, 374, 482, 693, 1369
BstV2I GAAGAC 1 cut(s) 92
Bsu36I CCTNAGG 1 cut(s) 1137
BsuRI GGCC 4 cut(s) 750, 1018, 1053, 1135
BtgZI GCGATG 1 cut(s) 522
BtsCI GGATG 7 cut(s) 80, 475, 651, 812, 904, 1015, 1487
BtsIMutI CAGTG 4 cut(s) 442, 908, 1203, 1576
BveI ACCTGC 1 cut(s) 1564
Cac8I GCNNGC 1 cut(s) 493
CfoI GCGC 1 cut(s) 327
Cfr13I GGNCC 3 cut(s) 59, 1133, 1216
CseI GACGC 2 cut(s) 755, 1496
Csp6I GTAC 2 cut(s) 636, 1553
CviQI GTAC 2 cut(s) 636, 1553
DdeI CTNAG 7 cut(s) 200, 480, 641, 911, 1070, 1137, 1533
DpnI GATC 4 cut(s) 91, 630, 1401, 1434
DpnII GATC 4 cut(s) 89, 628, 1399, 1432
DraIII CACNNNGTG 1 cut(s) 914
EaeI YGGCCR 1 cut(s) 748
Eam1104I CTCTTC 1 cut(s) 1312
EarI CTCTTC 1 cut(s) 1312
Ecl136II GAGCTC 1 cut(s) 206
Eco130I CCWWGG 2 cut(s) 285, 1276
Eco147I AGGCCT 1 cut(s) 1018
Eco24I GRGCYC 1 cut(s) 208
Eco47I GGWCC 2 cut(s) 59, 1216
Eco53kI GAGCTC 1 cut(s) 206
Eco57I CTGAAG 1 cut(s) 281
Eco81I CCTNAGG 1 cut(s) 1137
Eco91I GGTNACC 2 cut(s) 82, 923
EcoICRI GAGCTC 1 cut(s) 206
EcoO109I RGGNCCY 2 cut(s) 1133, 1216
EcoO65I GGTNACC 2 cut(s) 82, 923
EcoRII CCWGG 2 cut(s) 69, 1040
EcoT14I CCWWGG 2 cut(s) 285, 1276
EcoT38I GRGCYC 1 cut(s) 208
ErhI CCWWGG 2 cut(s) 285, 1276
Fnu4HI GCNGC 6 cut(s) 165, 168, 363, 496, 707, 1383
FokI GGATG 7 cut(s) 87, 482, 638, 799, 911, 1022, 1494
FriOI GRGCYC 1 cut(s) 208
Fsp4HI GCNGC 6 cut(s) 165, 168, 363, 496, 707, 1383
FspBI CTAG 2 cut(s) 104, 171
FspI TGCGCA 1 cut(s) 326
GlaI GCGC 1 cut(s) 326
GluI GCNGC 6 cut(s) 165, 168, 363, 496, 707, 1383
GsuI CTGGAG 1 cut(s) 848
HaeIII GGCC 4 cut(s) 750, 1018, 1053, 1135
HapII CCGG 1 cut(s) 87
HgaI GACGC 2 cut(s) 755, 1496
HhaI GCGC 1 cut(s) 327
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 3 cut(s) 611, 1096, 1247
HpaII CCGG 1 cut(s) 87
HphI GGTGA 5 cut(s) 76, 260, 629, 955, 1242
Hpy166II GTNNAC 4 cut(s) 216, 251, 849, 1414
Hpy188I TCNGA 8 cut(s) 49, 150, 300, 382, 451, 570, 587, 1426
Hpy188III TCNNGA 4 cut(s) 190, 479, 827, 1532
Hpy8I GTNNAC 4 cut(s) 216, 251, 849, 1414
HpyAV CCTTC 5 cut(s) 132, 889, 1068, 1402, 1486
HpyCH4III ACNGT 3 cut(s) 53, 1207, 1580
HpyF10VI GCNNNNNNNGC 3 cut(s) 273, 504, 1496
HpyF3I CTNAG 7 cut(s) 200, 480, 641, 911, 1070, 1137, 1533
HspAI GCGC 1 cut(s) 325
KpnI GGTACC 1 cut(s) 1556
Kzo9I GATC 4 cut(s) 89, 628, 1399, 1432
LmnI GCTCC 1 cut(s) 211
Lsp1109I GCAGC 5 cut(s) 151, 374, 482, 693, 1369
LweI GCATC 6 cut(s) 27, 526, 811, 1000, 1213, 1506
MaeI CTAG 2 cut(s) 104, 171
MaeIII GTNAC 9 cut(s) 37, 82, 590, 683, 806, 923, 961, 1387, 1484
MalI GATC 4 cut(s) 91, 630, 1401, 1434
MboI GATC 4 cut(s) 89, 628, 1399, 1432
MhlI GDGCHC 2 cut(s) 208, 910
MlsI TGGCCA 1 cut(s) 750
MluCI AATT 9 cut(s) 151, 802, 957, 974, 997, 1115, 1151, 1240, 1505
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 1 cut(s) 605
MmeI TCCRAC 1 cut(s) 569
Mox20I TGGCCA 1 cut(s) 750
MscI TGGCCA 1 cut(s) 750
MseI TTAA 9 cut(s) 126, 159, 462, 689, 918, 978, 996, 1352, 1374
MslI CAYNNNNRTG 3 cut(s) 262, 342, 786
Msp20I TGGCCA 1 cut(s) 750
MspI CCGG 1 cut(s) 87
MspR9I CCNGG 2 cut(s) 71, 1042
MvaI CCWGG 2 cut(s) 71, 1042
MvnI CGCG 1 cut(s) 1490
MwoI GCNNNNNNNGC 3 cut(s) 273, 504, 1496
NdeII GATC 4 cut(s) 89, 628, 1399, 1432
NlaIV GGNNCC 2 cut(s) 1217, 1554
NmuCI GTSAC 5 cut(s) 37, 82, 590, 961, 1484
NsbI TGCGCA 1 cut(s) 326
NspI RCATGY 3 cut(s) 267, 584, 1090
PceI AGGCCT 1 cut(s) 1018
PciI ACATGT 1 cut(s) 580
PfeI GAWTC 2 cut(s) 1096, 1247
PkrI GCNGC 6 cut(s) 166, 169, 364, 497, 708, 1384
PleI GAGTC 1 cut(s) 605
PpsI GAGTC 1 cut(s) 605
PpuMI RGGWCCY 1 cut(s) 1216
PscI ACATGT 1 cut(s) 580
PsiI TTATAA 3 cut(s) 731, 1034, 1594
Psp124BI GAGCTC 1 cut(s) 208
Psp5II RGGWCCY 1 cut(s) 1216
Psp6I CCWGG 2 cut(s) 69, 1040
PspEI GGTNACC 2 cut(s) 82, 923
PspGI CCWGG 2 cut(s) 69, 1040
PspN4I GGNNCC 2 cut(s) 1217, 1554
PspPI GGNCC 3 cut(s) 59, 1133, 1216
PspPPI RGGWCCY 1 cut(s) 1216
PstI CTGCAG 1 cut(s) 1387
RsaI GTAC 2 cut(s) 637, 1554
RsaNI GTAC 2 cut(s) 636, 1553
RseI CAYNNNNRTG 3 cut(s) 262, 342, 786
SacI GAGCTC 1 cut(s) 208
SaqAI TTAA 9 cut(s) 126, 159, 462, 689, 918, 978, 996, 1352, 1374
SatI GCNGC 6 cut(s) 165, 168, 363, 496, 707, 1383
Sau3AI GATC 4 cut(s) 89, 628, 1399, 1432
Sau96I GGNCC 3 cut(s) 59, 1133, 1216
SchI GAGTC 1 cut(s) 605
ScrFI CCNGG 2 cut(s) 71, 1042
SduI GDGCHC 2 cut(s) 208, 910
SfaNI GCATC 6 cut(s) 27, 526, 811, 1000, 1213, 1506
SfcI CTRYAG 1 cut(s) 1383
SinI GGWCC 2 cut(s) 59, 1216
SmiMI CAYNNNNRTG 3 cut(s) 262, 342, 786
SmlI CTYRAG 1 cut(s) 1128
SmoI CTYRAG 1 cut(s) 1128
Sse9I AATT 9 cut(s) 151, 802, 957, 974, 997, 1115, 1151, 1240, 1505
SseBI AGGCCT 1 cut(s) 1018
SsiI CCGC 2 cut(s) 167, 1253
SspMI CTAG 2 cut(s) 104, 171
SstI GAGCTC 1 cut(s) 208
StuI AGGCCT 1 cut(s) 1018
StyD4I CCNGG 2 cut(s) 69, 1040
StyI CCWWGG 2 cut(s) 285, 1276
TaaI ACNGT 3 cut(s) 53, 1207, 1580
TaqI TCGA 2 cut(s) 9, 436
TasI AATT 9 cut(s) 151, 802, 957, 974, 997, 1115, 1151, 1240, 1505
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 2 cut(s) 1096, 1247
Tru1I TTAA 9 cut(s) 126, 159, 462, 689, 918, 978, 996, 1352, 1374
Tru9I TTAA 9 cut(s) 126, 159, 462, 689, 918, 978, 996, 1352, 1374
TscAI CASTG 4 cut(s) 449, 915, 1210, 1583
TseFI GTSAC 5 cut(s) 37, 82, 590, 961, 1484
TseI GCWGC 5 cut(s) 164, 362, 495, 706, 1382
Tsp45I GTSAC 5 cut(s) 37, 82, 590, 961, 1484
TspDTI ATGAA 6 cut(s) 309, 726, 872, 1478, 1499, 1518
TspRI CASTG 4 cut(s) 449, 915, 1210, 1583
VpaK11BI GGWCC 2 cut(s) 59, 1216
XapI RAATTY 1 cut(s) 1505
XceI RCATGY 3 cut(s) 267, 584, 1090
XcmI CCANNNNNNNNNTGG 2 cut(s) 823, 896
XspI CTAG 2 cut(s) 104, 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.