Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0011022

Physical Location & Seq

Genomic Coordinates Reverse (-)

52569630 .. 52575836

Gene Structure 6207 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00120350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2124 bp

ATGATGGTCGATTTAGGCATCTCACTTGCAAGATTTGTGACCAGCTCGGACAGTTATGGTCCATTTCTTCTGGGATGGCTGGTCACCGGATCATTTGGTCTTCTAGCTATCATCTTTGCATTTCTTAAATGGGAGAGAAGGACATCTCTGAATTGGGTTAAAGCTGCGGCTAGAGAAAAGAAGAAAGTCTGGAAGAAGCTAAGAGCCCCTCTGTCACCCCATACATGGATAGAAGATATTACTAATGGTGAACAGCCATCTACATGCTGTGTTTGCTTGACTTCCTTGGTGTTTCTTCAGAACCTAGGCACTAAAGCTTCATCGCGCACTCCTGTCCATCGTTGTGTAGTTTGTGGTGTGGCAGCTCATTTTTATTGTTCTGATTTTGCAGTAAAAGATTGCAAGTGTGTGGCACAAGCTGGTTCTACCCATGTTCGACACCACTGGTCAGAAAAATGGGTTAATATGGATGATAATCCTGAGATTTCTGCCTGCTGCTTCTATTGCGATGAGCCATGTGGTGTTCCTTTCCTCGATGCTTCTCCAACATGGCATTGTCTATGGTGTCAGAACCTCATACATGTTAGATGTCACAATAAAATGGCAAAAGAGTCTGGTGATGTTTGTGATCTTGGTACTCTGAGGAGGGTCATCCTTTCTCCTTTATGTGTAAAAGAAGTTGGTCAATTAAATGGAACTGGAGTGCTGCGTTCAATAACAGATGAGTTTATAACTTCATCTGTTCGTGGCCAGTTTAGAAGAAAGCGTCATCGTTCTAAACATGGTAGTGGTCATTCAGCTAATTGTAACTCAAACAATGACGCTCTGGAGTATGTGTTCAATGGGCTTGCTGGTATGAAGAAGTCCCGTAGTGAGAAGAGTTTTGACCACTTGAAGGATGGCAGGGCACTGAGCGTTAAGGGTAACCATAATGGCTTTATGCAGAAAAAGGGTGGAATTGTCACCTATGGTCAATTTAAGAAATATAAAGTGGTTAATTTACCAAAGGATGCAAGGCCTCTTTTGGTTTTTATAAATGCCAGGAGTGGAGGCCAAAATGGGGCTTCTCTAAGGAGGAGGTTGAACATGCTATTGAATCCTGCACAGGTTTTTGAATTGAGTTCTTCTCAAGGGCCTGAGGTTGGTTTGGAATTGTTCAACGATGTGCAATACTTTAGAGTTTTGGTATGTGGTGGAGATGGCACTGTTGCATGGGTCCTTGATGCCATTGAGAAGCATAAATTTGAATCACCCCCACCTGTTGCTATTATTCCCCTTGGCACAGGAAATGATTTGTCAAGGGTTCTGCAATGGGGAAGAGGATTCTCTATCGTTGATGGACAAGGTGGCTTAACCACACTTTTACATGACATTAACCATGCTGCAGTTACAATGTTAGATCGCTGGAAAGTAAACATCAAATCAGAAGATGATCCAAATAAAGTGCAATCCAAGTTTATGATGAACTATTTAGGTATTGGATGTGACGCGAAGGTTGCTTATGAATTTCATGTGATGCGAGAAATAAATCCTGAGAAGTTCAGTAGTCAGTTTGTGAATAAACTGCGATATGCCAAAGAAGGTGCTAGAGATATAATGGACCGAACTTGTGCCGACTTACCATGGCAAGTTTGGCTTGAAGTTGATGGGAAAGACATAGATATTCCAAAGGATTCTGAGGGTCTAATCGTGCTCAACATTGGCAGCTACATGGGTGGAGTGGATCTTTGGCAAAATGATATTGATCATGATGATGACTTTAGTCTTCAGTCAATGCATGATAAAATACTTGAAGTTGTATGCATATGTGGATCATGGCACCTTGGCAAACTACAGGTTGGACTATCACAAGCTAGGCGACTAGCCCAGGGGAATATCATAAGAATACATGCATCTAGTCCTTTTCCAGTCCAAATAGATGGGGAGCCATATATACAACAACCTGGCTGTATAGAGATAACACATCATGGGCAGGTGTTTATGTTGAGGAGGGCTTCAGAGGAGCCGAGAGGGCATGCAGCTGCAATCATGACGGAGGTTTTATTAGACGCCGAGTGTAAAGGCATCATTAATGCATCTCAGAAGAAAACACTTCTCCAGCAGATGGCTCTCCAGCTTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004143 GO:0006793 GO:0006796 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009611 GO:0009628 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0022622 GO:0032501 GO:0032502 GO:0044237 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

C1_1 DAGK_acc DAGK_cat

Protein Analysis

707

Amino Acids

78.8

Weight (kDa)

8.53

Isoelectric Point (pI)

46.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C1_1	PF00130	147 - 206	1.1e-06	Phorbol esters/diacylglycerol binding domain (C1 domain)
DAGK_cat	PF00781	340 - 440	1e-26	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	486 - 643	2.1e-57	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011022)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63770 AT5G63770
fragaria_vesca	FvH4_1g25600 FvH4_1g25600 FvH4_1g25600
malus_domestica	MD15G1139400.v1.1
prunus_persica	Prupe.4G282200_v2.0.a1 Prupe.4G282200_v2.0.a1 Prupe.4G282200_v2.0.a1
pyrus_communis	pycom15g12580
rosa_chinensis	RchiOBHm_Chr2g0123771
rosa_laevigata	RLG00000018775
rosa_multiflora	Rmu_sc0001032.1_g000003
rosa_roxburghii	Rroxscaffold_2G00120350
rosa_rugosa	Rorug02G0247900
rosa_samantha	Rh2AG306800 Rh2BG315300 Rh2CG293200 Rh2DG330500
rosa_wichuraiana	Rw2G024620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	731, 1034
AarI	CACCTGC	1 cut(s)	1963
Acc36I	ACCTGC	1 cut(s)	1963
AccB1I	GGYRCC	1 cut(s)	1818
AccB7I	CCANNNNNTGG	1 cut(s)	2104
AccII	CGCG	2 cut(s)	325, 1490
AciI	CCGC	1 cut(s)	167
AclWI	GGATC	4 cut(s)	97, 1427, 1731, 1819
AcoI	YGGCCR	1 cut(s)	748
AcsI	RAATTY	2 cut(s)	1241, 1505
AcuI	CTGAAG	3 cut(s)	281, 1751, 1980
AcyI	GRCGYC	1 cut(s)	2049
AfaI	GTAC	1 cut(s)	637
AfiI	CCNNNNNNNGG	5 cut(s)	225, 895, 1060, 1142, 2104
AflIII	ACRYGT	1 cut(s)	580
AjnI	CCWGG	3 cut(s)	1040, 1866, 1942
AloI	GAACNNNNNNTCC	2 cut(s)	821, 853
Alw21I	GWGCWC	1 cut(s)	1695
AlwI	GGATC	4 cut(s)	97, 1427, 1731, 1819
AoxI	GGCC	4 cut(s)	748, 1016, 1051, 1133
ApeKI	GCWGC	8 cut(s)	164, 362, 495, 706, 1382, 1704, 2018, 2021
ApoI	RAATTY	2 cut(s)	1241, 1505
AseI	ATTAAT	1 cut(s)	2070
AspA2I	CCTAGG	1 cut(s)	304
AspLEI	GCGC	1 cut(s)	327
AspS9I	GGNCC	4 cut(s)	59, 1133, 1216, 1600
AsuHPI	GGTGA	6 cut(s)	76, 207, 260, 629, 955, 1242
AvaII	GGWCC	3 cut(s)	59, 1216, 1600
AvrII	CCTAGG	1 cut(s)	304
AxyI	CCTNAGG	1 cut(s)	1137
BaeGI	GKGCMC	1 cut(s)	910
BalI	TGGCCA	1 cut(s)	750
BanI	GGYRCC	1 cut(s)	1818
BanII	GRGCYC	1 cut(s)	208
BbsI	GAAGAC	2 cut(s)	92, 1757
Bbv12I	GWGCWC	1 cut(s)	1695
BbvI	GCAGC	8 cut(s)	151, 374, 482, 693, 1369, 1716, 2008, 2030
BccI	CCATC	9 cut(s)	69, 265, 345, 893, 1193, 1331, 1640, 1913, 2098
BciT130I	CCWGG	3 cut(s)	1042, 1868, 1944
BclI	TGATCA	1 cut(s)	1744
BfaI	CTAG	7 cut(s)	104, 171, 305, 1587, 1854, 1862, 1896
BfmI	CTRYAG	2 cut(s)	1383, 1832
BfuAI	ACCTGC	1 cut(s)	1963
BglI	GCCNNNNNGGC	1 cut(s)	2011
BisI	GCNGC	9 cut(s)	165, 168, 363, 496, 707, 1383, 1705, 2019, 2022
BlnI	CCTAGG	1 cut(s)	304
BlsI	GCNGC	9 cut(s)	166, 169, 364, 497, 708, 1384, 1706, 2020, 2023
Bme1390I	CCNGG	3 cut(s)	1042, 1868, 1944
Bme18I	GGWCC	3 cut(s)	59, 1216, 1600
BmgT120I	GGNCC	4 cut(s)	59, 1133, 1216, 1600
BmiI	GGNNCC	4 cut(s)	1217, 1820, 1926, 2004
BmrFI	CCNGG	3 cut(s)	1042, 1868, 1944
BmsI	GCATC	8 cut(s)	27, 526, 1000, 1213, 1506, 1901, 2073, 2084
BoxI	GACNNNNGTC	1 cut(s)	1761
BpiI	GAAGAC	2 cut(s)	92, 1757
BplI	GAGNNNNNCTC	2 cut(s)	1111, 1143
BpmI	CTGGAG	4 cut(s)	720, 848, 2081, 2096
BpuEI	CTTGAG	1 cut(s)	1113
BsaBI	GATNNNNATC	2 cut(s)	474, 1743
BsaHI	GRCGYC	1 cut(s)	2049
BsaJI	CCNNGG	7 cut(s)	285, 304, 1276, 1622, 1822, 1866, 1867
BsaWI	WCCGGW	1 cut(s)	86
BsaXI	ACNNNNNCTCC	2 cut(s)	821, 851
Bsc4I	CCNNNNNNNGG	5 cut(s)	225, 895, 1060, 1142, 2104
Bse1I	ACTGG	4 cut(s)	449, 703, 751, 1907
Bse21I	CCTNAGG	1 cut(s)	1137
Bse3DI	GCAATG	1 cut(s)	1316
Bse8I	GATNNNNATC	2 cut(s)	474, 1743
BseBI	CCWGG	3 cut(s)	1042, 1868, 1944
BseDI	CCNNGG	7 cut(s)	285, 304, 1276, 1622, 1822, 1866, 1867
BseGI	GGATG	6 cut(s)	80, 475, 651, 904, 1015, 1487
BseJI	GATNNNNATC	2 cut(s)	474, 1743
BseLI	CCNNNNNNNGG	5 cut(s)	225, 895, 1060, 1142, 2104
BseMI	GCAATG	1 cut(s)	1316
BseMII	CTCAG	7 cut(s)	471, 632, 902, 1128, 1524, 1668, 2093
BseNI	ACTGG	4 cut(s)	449, 703, 751, 1907
BseRI	GAGGAG	4 cut(s)	658, 1090, 2002, 2015
BseSI	GKGCMC	1 cut(s)	910
BseXI	GCAGC	8 cut(s)	151, 374, 482, 693, 1369, 1716, 2008, 2030
BsgI	GTGCAG	1 cut(s)	1086
Bsh1236I	CGCG	2 cut(s)	325, 1490
BshFI	GGCC	4 cut(s)	750, 1018, 1053, 1135
BshNI	GGYRCC	1 cut(s)	1818
BsiHKAI	GWGCWC	1 cut(s)	1695
BsiSI	CCGG	1 cut(s)	87
BslFI	GGGAC	1 cut(s)	850
BslI	CCNNNNNNNGG	5 cut(s)	225, 895, 1060, 1142, 2104
BsmFI	GGGAC	1 cut(s)	850
BsnI	GGCC	4 cut(s)	750, 1018, 1053, 1135
Bsp1286I	GDGCHC	3 cut(s)	208, 910, 1695
Bsp143I	GATC	7 cut(s)	89, 628, 1399, 1432, 1723, 1744, 1811
Bsp19I	CCATGG	1 cut(s)	1622
BspACI	CCGC	1 cut(s)	167
BspANI	GGCC	4 cut(s)	750, 1018, 1053, 1135
BspCNI	CTCAG	7 cut(s)	472, 633, 903, 1129, 1525, 1669, 2092
BspFNI	CGCG	2 cut(s)	325, 1490
BspHI	TCATGA	2 cut(s)	1747, 2028
BspLI	GGNNCC	4 cut(s)	1217, 1820, 1926, 2004
BspMAI	CTGCAG	1 cut(s)	1387
BspMI	ACCTGC	1 cut(s)	1963
BspPI	GGATC	4 cut(s)	97, 1427, 1731, 1819
BspT107I	GGYRCC	1 cut(s)	1818
BsrDI	GCAATG	1 cut(s)	1316
BsrI	ACTGG	4 cut(s)	449, 703, 751, 1907
BssECI	CCNNGG	7 cut(s)	285, 304, 1276, 1622, 1822, 1866, 1867
BssMI	GATC	7 cut(s)	89, 628, 1399, 1432, 1723, 1744, 1811
BssNI	GRCGYC	1 cut(s)	2049
BssT1I	CCWWGG	5 cut(s)	285, 304, 1276, 1622, 1822
Bst2UI	CCWGG	3 cut(s)	1042, 1868, 1944
Bst4CI	ACNGT	2 cut(s)	53, 1207
Bst6I	CTCTTC	2 cut(s)	872, 1312
BstACI	GRCGYC	1 cut(s)	2049
BstC8I	GCNNGC	4 cut(s)	493, 849, 2016, 2118
BstDEI	CTNAG	9 cut(s)	200, 480, 641, 911, 1070, 1137, 1533, 1677, 2079
BstDSI	CCRYGG	1 cut(s)	1622
BstEII	GGTNACC	2 cut(s)	82, 923
BstF5I	GGATG	6 cut(s)	80, 475, 651, 904, 1015, 1487
BstFNI	CGCG	2 cut(s)	325, 1490
BstHHI	GCGC	1 cut(s)	327
BstKTI	GATC	7 cut(s)	92, 631, 1402, 1435, 1726, 1747, 1814
BstMBI	GATC	7 cut(s)	89, 628, 1399, 1432, 1723, 1744, 1811
BstMWI	GCNNNNNNNGC	5 cut(s)	273, 504, 1496, 1633, 2011
BstNI	CCWGG	3 cut(s)	1042, 1868, 1944
BstNSI	RCATGY	5 cut(s)	267, 584, 1090, 1892, 2018
BstPAI	GACNNNNGTC	1 cut(s)	1761
BstPI	GGTNACC	2 cut(s)	82, 923
BstSCI	CCNGG	3 cut(s)	1040, 1866, 1942
BstSFI	CTRYAG	2 cut(s)	1383, 1832
BstSLI	GKGCMC	1 cut(s)	910
BstUI	CGCG	2 cut(s)	325, 1490
BstV1I	GCAGC	8 cut(s)	151, 374, 482, 693, 1369, 1716, 2008, 2030
BstV2I	GAAGAC	2 cut(s)	92, 1757
BstX2I	RGATCY	1 cut(s)	1723
BstXI	CCANNNNNNTGG	1 cut(s)	1919
BstYI	RGATCY	1 cut(s)	1723
Bsu36I	CCTNAGG	1 cut(s)	1137
BsuRI	GGCC	4 cut(s)	750, 1018, 1053, 1135
BtgI	CCRYGG	1 cut(s)	1622
BtgZI	GCGATG	2 cut(s)	306, 522
BtsCI	GGATG	6 cut(s)	80, 475, 651, 904, 1015, 1487
BtsIMutI	CAGTG	3 cut(s)	442, 908, 1203
BveI	ACCTGC	1 cut(s)	1963
Cac8I	GCNNGC	4 cut(s)	493, 849, 2016, 2118
CciI	TCATGA	2 cut(s)	1747, 2028
CfoI	GCGC	1 cut(s)	327
Cfr13I	GGNCC	4 cut(s)	59, 1133, 1216, 1600
CseI	GACGC	4 cut(s)	755, 830, 1496, 2057
Csp6I	GTAC	1 cut(s)	636
CviQI	GTAC	1 cut(s)	636
DdeI	CTNAG	9 cut(s)	200, 480, 641, 911, 1070, 1137, 1533, 1677, 2079
DpnI	GATC	7 cut(s)	91, 630, 1401, 1434, 1725, 1746, 1813
DpnII	GATC	7 cut(s)	89, 628, 1399, 1432, 1723, 1744, 1811
EaeI	YGGCCR	1 cut(s)	748
Eam1104I	CTCTTC	2 cut(s)	872, 1312
EarI	CTCTTC	2 cut(s)	872, 1312
Eco130I	CCWWGG	5 cut(s)	285, 304, 1276, 1622, 1822
Eco147I	AGGCCT	1 cut(s)	1018
Eco24I	GRGCYC	1 cut(s)	208
Eco47I	GGWCC	3 cut(s)	59, 1216, 1600
Eco57I	CTGAAG	3 cut(s)	281, 1751, 1980
Eco81I	CCTNAGG	1 cut(s)	1137
Eco91I	GGTNACC	2 cut(s)	82, 923
EcoO109I	RGGNCCY	2 cut(s)	1133, 1216
EcoO65I	GGTNACC	2 cut(s)	82, 923
EcoRII	CCWGG	3 cut(s)	1040, 1866, 1942
EcoT14I	CCWWGG	5 cut(s)	285, 304, 1276, 1622, 1822
EcoT22I	ATGCAT	4 cut(s)	1779, 1805, 1894, 2077
EcoT38I	GRGCYC	1 cut(s)	208
ErhI	CCWWGG	5 cut(s)	285, 304, 1276, 1622, 1822
FalI	AAGNNNNNCTT	2 cut(s)	1620, 1652
FaqI	GGGAC	1 cut(s)	850
FauNDI	CATATG	1 cut(s)	1805
FbaI	TGATCA	1 cut(s)	1744
Fnu4HI	GCNGC	9 cut(s)	165, 168, 363, 496, 707, 1383, 1705, 2019, 2022
FokI	GGATG	6 cut(s)	87, 482, 638, 911, 1022, 1494
FriOI	GRGCYC	1 cut(s)	208
Fsp4HI	GCNGC	9 cut(s)	165, 168, 363, 496, 707, 1383, 1705, 2019, 2022
FspBI	CTAG	7 cut(s)	104, 171, 305, 1587, 1854, 1862, 1896
GlaI	GCGC	1 cut(s)	326
GluI	GCNGC	9 cut(s)	165, 168, 363, 496, 707, 1383, 1705, 2019, 2022
GsuI	CTGGAG	4 cut(s)	720, 848, 2081, 2096
HaeIII	GGCC	4 cut(s)	750, 1018, 1053, 1135
HapII	CCGG	1 cut(s)	87
HgaI	GACGC	4 cut(s)	755, 830, 1496, 2057
HhaI	GCGC	1 cut(s)	327
Hin1I	GRCGYC	1 cut(s)	2049
Hin6I	GCGC	1 cut(s)	325
HinP1I	GCGC	1 cut(s)	325
HindIII	AAGCTT	1 cut(s)	315
HinfI	GANTC	5 cut(s)	611, 1096, 1247, 1323, 1673
HpaII	CCGG	1 cut(s)	87
HphI	GGTGA	6 cut(s)	76, 207, 260, 629, 955, 1242
Hpy166II	GTNNAC	2 cut(s)	251, 1414
Hpy188III	TCNNGA	6 cut(s)	190, 479, 827, 1532, 1748, 2029
Hpy8I	GTNNAC	2 cut(s)	251, 1414
HpyAV	CCTTC	4 cut(s)	132, 889, 1486, 1574
HpyCH4III	ACNGT	2 cut(s)	53, 1207
HpyF10VI	GCNNNNNNNGC	5 cut(s)	273, 504, 1496, 1633, 2011
HpyF3I	CTNAG	9 cut(s)	200, 480, 641, 911, 1070, 1137, 1533, 1677, 2079
Hsp92I	GRCGYC	1 cut(s)	2049
HspAI	GCGC	1 cut(s)	325
Ksp22I	TGATCA	1 cut(s)	1744
Kzo9I	GATC	7 cut(s)	89, 628, 1399, 1432, 1723, 1744, 1811
LmnI	GCTCC	2 cut(s)	1924, 2002
Lsp1109I	GCAGC	8 cut(s)	151, 374, 482, 693, 1369, 1716, 2008, 2030
LweI	GCATC	8 cut(s)	27, 526, 1000, 1213, 1506, 1901, 2073, 2084
MaeI	CTAG	7 cut(s)	104, 171, 305, 1587, 1854, 1862, 1896
MaeIII	GTNAC	9 cut(s)	37, 82, 213, 590, 806, 923, 961, 1387, 1484
MalI	GATC	7 cut(s)	91, 630, 1401, 1434, 1725, 1746, 1813
MboI	GATC	7 cut(s)	89, 628, 1399, 1432, 1723, 1744, 1811
MflI	RGATCY	1 cut(s)	1723
MhlI	GDGCHC	3 cut(s)	208, 910, 1695
MlsI	TGGCCA	1 cut(s)	750
MluNI	TGGCCA	1 cut(s)	750
MlyI	GAGTC	1 cut(s)	620
MmeI	TCCRAC	2 cut(s)	569, 1819
Mox20I	TGGCCA	1 cut(s)	750
Mph1103I	ATGCAT	4 cut(s)	1779, 1805, 1894, 2077
MscI	TGGCCA	1 cut(s)	750
MslI	CAYNNNNRTG	4 cut(s)	262, 342, 786, 1752
Msp20I	TGGCCA	1 cut(s)	750
MspA1I	CMGCKG	1 cut(s)	2021
MspI	CCGG	1 cut(s)	87
MspR9I	CCNGG	3 cut(s)	1042, 1868, 1944
MvaI	CCWGG	3 cut(s)	1042, 1868, 1944
MvnI	CGCG	2 cut(s)	325, 1490
MwoI	GCNNNNNNNGC	5 cut(s)	273, 504, 1496, 1633, 2011
NcoI	CCATGG	1 cut(s)	1622
NdeI	CATATG	1 cut(s)	1805
NdeII	GATC	7 cut(s)	89, 628, 1399, 1432, 1723, 1744, 1811
NlaIV	GGNNCC	4 cut(s)	1217, 1820, 1926, 2004
NmeAIII	GCCGAG	2 cut(s)	2031, 2077
NmuCI	GTSAC	6 cut(s)	37, 82, 213, 590, 961, 1484
NsiI	ATGCAT	4 cut(s)	1779, 1805, 1894, 2077
NspI	RCATGY	5 cut(s)	267, 584, 1090, 1892, 2018
PaeI	GCATGC	1 cut(s)	2018
PagI	TCATGA	2 cut(s)	1747, 2028
PaqCI	CACCTGC	1 cut(s)	1963
PasI	CCCWGGG	1 cut(s)	1867
PceI	AGGCCT	1 cut(s)	1018
PciI	ACATGT	1 cut(s)	580
PfeI	GAWTC	4 cut(s)	1096, 1247, 1323, 1673
PflMI	CCANNNNNTGG	1 cut(s)	2104
PkrI	GCNGC	9 cut(s)	166, 169, 364, 497, 708, 1384, 1706, 2020, 2023
PleI	GAGTC	1 cut(s)	619
PpsI	GAGTC	1 cut(s)	619
PpuMI	RGGWCCY	1 cut(s)	1216
PscI	ACATGT	1 cut(s)	580
PshAI	GACNNNNGTC	1 cut(s)	1761
PshBI	ATTAAT	1 cut(s)	2070
PsiI	TTATAA	2 cut(s)	731, 1034
Psp5II	RGGWCCY	1 cut(s)	1216
Psp6I	CCWGG	3 cut(s)	1040, 1866, 1942
PspEI	GGTNACC	2 cut(s)	82, 923
PspGI	CCWGG	3 cut(s)	1040, 1866, 1942
PspN4I	GGNNCC	4 cut(s)	1217, 1820, 1926, 2004
PspPI	GGNCC	4 cut(s)	59, 1133, 1216, 1600
PspPPI	RGGWCCY	1 cut(s)	1216
PstI	CTGCAG	1 cut(s)	1387
PsuI	RGATCY	1 cut(s)	1723
PvuII	CAGCTG	1 cut(s)	2021
RsaI	GTAC	1 cut(s)	637
RsaNI	GTAC	1 cut(s)	636
RseI	CAYNNNNRTG	4 cut(s)	262, 342, 786, 1752
SatI	GCNGC	9 cut(s)	165, 168, 363, 496, 707, 1383, 1705, 2019, 2022
Sau3AI	GATC	7 cut(s)	89, 628, 1399, 1432, 1723, 1744, 1811
Sau96I	GGNCC	4 cut(s)	59, 1133, 1216, 1600
SchI	GAGTC	1 cut(s)	620
ScrFI	CCNGG	3 cut(s)	1042, 1868, 1944
SduI	GDGCHC	3 cut(s)	208, 910, 1695
SfaNI	GCATC	8 cut(s)	27, 526, 1000, 1213, 1506, 1901, 2073, 2084
SfcI	CTRYAG	2 cut(s)	1383, 1832
SinI	GGWCC	3 cut(s)	59, 1216, 1600
SmiMI	CAYNNNNRTG	4 cut(s)	262, 342, 786, 1752
SmlI	CTYRAG	1 cut(s)	1128
SmoI	CTYRAG	1 cut(s)	1128
SphI	GCATGC	1 cut(s)	2018
SseBI	AGGCCT	1 cut(s)	1018
SsiI	CCGC	1 cut(s)	167
SspMI	CTAG	7 cut(s)	104, 171, 305, 1587, 1854, 1862, 1896
StuI	AGGCCT	1 cut(s)	1018
StyD4I	CCNGG	3 cut(s)	1040, 1866, 1942
StyI	CCWWGG	5 cut(s)	285, 304, 1276, 1622, 1822
TaaI	ACNGT	2 cut(s)	53, 1207
TaqI	TCGA	3 cut(s)	9, 436, 534
TaqII	GACCGA	1 cut(s)	1617
TauI	GCSGC	1 cut(s)	170
TfiI	GAWTC	4 cut(s)	1096, 1247, 1323, 1673
TscAI	CASTG	3 cut(s)	449, 915, 1210
TseFI	GTSAC	6 cut(s)	37, 82, 213, 590, 961, 1484
TseI	GCWGC	8 cut(s)	164, 362, 495, 706, 1382, 1704, 2018, 2021
Tsp45I	GTSAC	6 cut(s)	37, 82, 213, 590, 961, 1484
TspDTI	ATGAA	6 cut(s)	309, 726, 872, 1478, 1499, 1518
TspGWI	ACGGA	1 cut(s)	2048
TspRI	CASTG	3 cut(s)	449, 915, 1210
Van91I	CCANNNNNTGG	1 cut(s)	2104
VpaK11BI	GGWCC	3 cut(s)	59, 1216, 1600
VspI	ATTAAT	1 cut(s)	2070
XapI	RAATTY	2 cut(s)	1241, 1505
XceI	RCATGY	5 cut(s)	267, 584, 1090, 1892, 2018
XcmI	CCANNNNNNNNNTGG	2 cut(s)	896, 1629
XmaJI	CCTAGG	1 cut(s)	304
XspI	CTAG	7 cut(s)	104, 171, 305, 1587, 1854, 1862, 1896
Zsp2I	ATGCAT	4 cut(s)	1779, 1805, 1894, 2077

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.