Rorug02G0247900

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0011022
Physical Location & Seq
Forward (+)
25856648 .. 25857439
792 bp
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UTR
Exon/CDS
Intron
Rorug02G0247900.1

Sequence Viewer

Length: 792 bp
ATGCTAGCCAGGGTTAGGGATTTTATTAAGAATAAGCAATGGACAATTCCTCCTATTTTTTCTTTCTTCTTTCCAGATCTTGCTTCACAAATTTCTAAGGTGGTGTTGCCTCTGGAAAGCGTGGGGGACCAAGTCATTTGGCCATCGAACGCAGCTGGAGTTTTAACAATAAAGGATGCTTTCATGTTTCTCTCACCACCTAGGCCTTCAGTCAACTGGTGCAAATTGGTTTGGAATAAGGCTTTGCAGCCCCGCAAAAGCTTGGTGACTTGGAATGTCTTAAACAAGAGGATTTTAACTGATGATTTATTAGTAAGACGGGGAATCTCTCTTTGTTCTCAGTGCTCTATTTGCCGAAGACAAGGTGAGTCTCTAAATCATCTTTTCTTTGAGTGCTATTTAATCCATGATTTCTGGAGTTGGCTTGCTTCTTTTTTCAGCAAGAGGATTCTGCCAGGTACTTCCCTTGTTGATCTTCTCACTCTAGACTTTCTAAATGGGATGGCTAGCTCTTCAAAACTTCTATGGAGTTTTGCTTTCTGTAATGCTGTCTGGTGCATATGGACAGAGAGAAACAAAATCGGGTTTCATGAGACTACTTTTAATTTAGCAAGGTTCCAGCATTGGTTTCTAATTTCTTTGAGAGAGTCTGCCTCCATTCACTTTCTGCCGTGCTCTAATTCTTTGAGAATGCAAGGTTTATTTGCTGTTCTCGGGCTTTCTTCTTTGACTATAAAGGCTCCAAAATGTATTCTAGTACAATGGACTCCCCCCCCCCAGCTCATTGGTTGA

Protein Analysis

263

Amino Acids

30.16

Weight (kDa)

9.58

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 56 - 139 1.9e-20 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011022)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63770 AT5G63770
fragaria_vesca FvH4_1g25600 FvH4_1g25600 FvH4_1g25600
malus_domestica MD15G1139400.v1.1
prunus_persica Prupe.4G282200_v2.0.a1 Prupe.4G282200_v2.0.a1 Prupe.4G282200_v2.0.a1
pyrus_communis pycom15g12580
rosa_chinensis RchiOBHm_Chr2g0123771
rosa_laevigata RLG00000018775
rosa_multiflora Rmu_sc0001032.1_g000003
rosa_roxburghii Rroxscaffold_2G00120350
rosa_rugosa Rorug02G0247900
rosa_samantha Rh2AG306800 Rh2BG315300 Rh2CG293200 Rh2DG330500
rosa_wichuraiana Rw2G024620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 253
AcoI YGGCCR 1 cut(s) 140
AcsI RAATTY 1 cut(s) 90
AcuI CTGAAG 1 cut(s) 192
AfaI GTAC 2 cut(s) 460, 759
AfiI CCNNNNNNNGG 1 cut(s) 15
AgsI TTSAA 1 cut(s) 516
AjnI CCWGG 2 cut(s) 8, 454
AluBI AGCT 4 cut(s) 155, 261, 510, 781
AluI AGCT 4 cut(s) 155, 261, 510, 781
Alw21I GWGCWC 2 cut(s) 347, 677
Alw26I GTCTC 2 cut(s) 375, 587
Ama87I CYCGRG 1 cut(s) 713
AoxI GGCC 2 cut(s) 140, 203
ApeKI GCWGC 2 cut(s) 152, 247
ApoI RAATTY 1 cut(s) 90
AspA2I CCTAGG 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 127
AsuHPI GGTGA 3 cut(s) 186, 277, 377
AsuNHI GCTAGC 2 cut(s) 4, 506
AvaI CYCGRG 1 cut(s) 713
AvaII GGWCC 1 cut(s) 127
AvrII CCTAGG 1 cut(s) 200
BalI TGGCCA 1 cut(s) 142
BbsI GAAGAC 1 cut(s) 364
Bbv12I GWGCWC 2 cut(s) 347, 677
BbvI GCAGC 2 cut(s) 164, 259
BccI CCATC 2 cut(s) 151, 496
BceAI ACGGC 1 cut(s) 655
BciT130I CCWGG 2 cut(s) 10, 456
BcoDI GTCTC 2 cut(s) 375, 587
BfaI CTAG 5 cut(s) 5, 201, 485, 507, 755
BglII AGATCT 1 cut(s) 76
BisI GCNGC 2 cut(s) 153, 248
BlnI CCTAGG 1 cut(s) 200
BlsI GCNGC 2 cut(s) 154, 249
Bme1390I CCNGG 2 cut(s) 10, 456
Bme18I GGWCC 1 cut(s) 127
BmeT110I CYCGRG 1 cut(s) 713
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 3 cut(s) 128, 617, 741
BmrFI CCNGG 2 cut(s) 10, 456
BmsI GCATC 1 cut(s) 166
BmtI GCTAGC 2 cut(s) 8, 510
BpiI GAAGAC 1 cut(s) 364
BplI GAGNNNNNCTC 2 cut(s) 638, 670
BpmI CTGGAG 2 cut(s) 177, 436
BsaJI CCNNGG 2 cut(s) 9, 200
BsaXI ACNNNNNCTCC 2 cut(s) 34, 64
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse1I ACTGG 1 cut(s) 221
Bse3DI GCAATG 1 cut(s) 44
BseBI CCWGG 2 cut(s) 10, 456
BseDI CCNNGG 2 cut(s) 9, 200
BseGI GGATG 2 cut(s) 181, 507
BseLI CCNNNNNNNGG 1 cut(s) 15
BseMI GCAATG 1 cut(s) 44
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 1 cut(s) 221
BseXI GCAGC 2 cut(s) 164, 259
BseYI CCCAGC 1 cut(s) 777
BshFI GGCC 2 cut(s) 142, 205
BsiHKAI GWGCWC 2 cut(s) 347, 677
BsiHKCI CYCGRG 1 cut(s) 713
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 15
BsmAI GTCTC 2 cut(s) 375, 587
BsmFI GGGAC 1 cut(s) 140
BsmI GAATGC 1 cut(s) 696
BsnI GGCC 2 cut(s) 142, 205
BsoBI CYCGRG 1 cut(s) 713
Bsp1286I GDGCHC 2 cut(s) 347, 677
Bsp143I GATC 2 cut(s) 76, 472
BspACI CCGC 1 cut(s) 253
BspANI GGCC 2 cut(s) 142, 205
BspCNI CTCAG 1 cut(s) 352
BspHI TCATGA 1 cut(s) 589
BspLI GGNNCC 3 cut(s) 128, 617, 741
BspOI GCTAGC 2 cut(s) 8, 510
BspQI GCTCTTC 1 cut(s) 517
BsrDI GCAATG 1 cut(s) 44
BsrI ACTGG 1 cut(s) 221
BssECI CCNNGG 2 cut(s) 9, 200
BssMI GATC 2 cut(s) 76, 472
BssT1I CCWWGG 1 cut(s) 200
Bst2UI CCWGG 2 cut(s) 10, 456
Bst6I CTCTTC 1 cut(s) 517
BstC8I GCNNGC 3 cut(s) 6, 426, 508
BstDEI CTNAG 2 cut(s) 96, 339
BstF5I GGATG 2 cut(s) 181, 507
BstKTI GATC 2 cut(s) 79, 475
BstMAI GTCTC 2 cut(s) 375, 587
BstMBI GATC 2 cut(s) 76, 472
BstMWI GCNNNNNNNGC 1 cut(s) 351
BstNI CCWGG 2 cut(s) 10, 456
BstSCI CCNGG 2 cut(s) 8, 454
BstV1I GCAGC 2 cut(s) 164, 259
BstV2I GAAGAC 1 cut(s) 364
BstX2I RGATCY 1 cut(s) 76
BstXI CCANNNNNNTGG 1 cut(s) 785
BstYI RGATCY 1 cut(s) 76
BsuRI GGCC 2 cut(s) 142, 205
BtsCI GGATG 2 cut(s) 181, 507
BtsIMutI CAGTG 1 cut(s) 347
Cac8I GCNNGC 3 cut(s) 6, 426, 508
CciI TCATGA 1 cut(s) 589
Cfr13I GGNCC 1 cut(s) 127
Csp6I GTAC 2 cut(s) 459, 758
CviAII CATG 3 cut(s) 184, 407, 590
CviQI GTAC 2 cut(s) 459, 758
DdeI CTNAG 2 cut(s) 96, 339
DpnI GATC 2 cut(s) 78, 474
DpnII GATC 2 cut(s) 76, 472
EaeI YGGCCR 1 cut(s) 140
Eam1104I CTCTTC 1 cut(s) 517
EarI CTCTTC 1 cut(s) 517
Eco130I CCWWGG 1 cut(s) 200
Eco147I AGGCCT 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 713
EcoRII CCWGG 2 cut(s) 8, 454
EcoT14I CCWWGG 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 200
FaeI CATG 3 cut(s) 187, 410, 593
FaiI YATR 7 cut(s) 185, 408, 526, 560, 562, 591, 734
FaqI GGGAC 1 cut(s) 140
FatI CATG 3 cut(s) 183, 406, 589
FauI CCCGC 1 cut(s) 260
FauNDI CATATG 1 cut(s) 560
Fnu4HI GCNGC 2 cut(s) 153, 248
FokI GGATG 2 cut(s) 188, 514
Fsp4HI GCNGC 2 cut(s) 153, 248
FspBI CTAG 5 cut(s) 5, 201, 485, 507, 755
GluI GCNGC 2 cut(s) 153, 248
GsaI CCCAGC 1 cut(s) 781
GsuI CTGGAG 2 cut(s) 177, 436
HaeIII GGCC 2 cut(s) 142, 205
Hin1II CATG 3 cut(s) 187, 410, 593
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 259
HinfI GANTC 5 cut(s) 324, 368, 448, 647, 766
HphI GGTGA 3 cut(s) 186, 277, 377
Hpy166II GTNNAC 1 cut(s) 214
Hpy188III TCNNGA 5 cut(s) 74, 113, 415, 485, 590
Hpy8I GTNNAC 1 cut(s) 214
HpyAV CCTTC 1 cut(s) 216
HpyCH4V TGCA 4 cut(s) 222, 247, 558, 694
HpyF10VI GCNNNNNNNGC 1 cut(s) 351
HpyF3I CTNAG 2 cut(s) 96, 339
Hsp92II CATG 3 cut(s) 187, 410, 593
Kzo9I GATC 2 cut(s) 76, 472
LguI GCTCTTC 1 cut(s) 517
LmnI GCTCC 1 cut(s) 745
Lsp1109I GCAGC 2 cut(s) 164, 259
LweI GCATC 1 cut(s) 166
MaeI CTAG 5 cut(s) 5, 201, 485, 507, 755
MaeIII GTNAC 1 cut(s) 265
MalI GATC 2 cut(s) 78, 474
MboI GATC 2 cut(s) 76, 472
MboII GAAGA 5 cut(s) 58, 369, 467, 504, 714
MflI RGATCY 1 cut(s) 76
MhlI GDGCHC 2 cut(s) 347, 677
MlsI TGGCCA 1 cut(s) 142
MluCI AATT 6 cut(s) 45, 90, 224, 604, 633, 679
MluNI TGGCCA 1 cut(s) 142
MlyI GAGTC 3 cut(s) 377, 656, 760
MnlI CCTC 5 cut(s) 60, 120, 282, 438, 664
Mox20I TGGCCA 1 cut(s) 142
MscI TGGCCA 1 cut(s) 142
MseI TTAA 6 cut(s) 27, 164, 281, 296, 401, 603
Msp20I TGGCCA 1 cut(s) 142
MspA1I CMGCKG 1 cut(s) 155
MspR9I CCNGG 2 cut(s) 10, 456
Mva1269I GAATGC 1 cut(s) 696
MvaI CCWGG 2 cut(s) 10, 456
MwoI GCNNNNNNNGC 1 cut(s) 351
NdeI CATATG 1 cut(s) 560
NdeII GATC 2 cut(s) 76, 472
NheI GCTAGC 2 cut(s) 4, 506
NlaIII CATG 3 cut(s) 187, 410, 593
NlaIV GGNNCC 3 cut(s) 128, 617, 741
NmuCI GTSAC 1 cut(s) 265
PagI TCATGA 1 cut(s) 589
PceI AGGCCT 1 cut(s) 205
PciSI GCTCTTC 1 cut(s) 517
PctI GAATGC 1 cut(s) 696
PfeI GAWTC 2 cut(s) 324, 448
PflFI GACNNNGTC 1 cut(s) 131
PkrI GCNGC 2 cut(s) 154, 249
PleI GAGTC 3 cut(s) 376, 655, 760
PpsI GAGTC 3 cut(s) 376, 655, 760
Psp6I CCWGG 2 cut(s) 8, 454
PspFI CCCAGC 1 cut(s) 777
PspGI CCWGG 2 cut(s) 8, 454
PspN4I GGNNCC 3 cut(s) 128, 617, 741
PspPI GGNCC 1 cut(s) 127
PsuI RGATCY 1 cut(s) 76
PsyI GACNNNGTC 1 cut(s) 131
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 2 cut(s) 460, 759
RsaNI GTAC 2 cut(s) 459, 758
SapI GCTCTTC 1 cut(s) 517
SaqAI TTAA 6 cut(s) 27, 164, 281, 296, 401, 603
SatI GCNGC 2 cut(s) 153, 248
Sau3AI GATC 2 cut(s) 76, 472
Sau96I GGNCC 1 cut(s) 127
SchI GAGTC 3 cut(s) 377, 656, 760
ScrFI CCNGG 2 cut(s) 10, 456
SduI GDGCHC 2 cut(s) 347, 677
SfaNI GCATC 1 cut(s) 166
SinI GGWCC 1 cut(s) 127
Sse9I AATT 6 cut(s) 45, 90, 224, 604, 633, 679
SseBI AGGCCT 1 cut(s) 205
SsiI CCGC 1 cut(s) 253
SspMI CTAG 5 cut(s) 5, 201, 485, 507, 755
StuI AGGCCT 1 cut(s) 205
StyD4I CCNGG 2 cut(s) 8, 454
StyI CCWWGG 1 cut(s) 200
TaqI TCGA 1 cut(s) 146
TasI AATT 6 cut(s) 45, 90, 224, 604, 633, 679
TatI WGTACW 1 cut(s) 757
TfiI GAWTC 2 cut(s) 324, 448
Tru1I TTAA 6 cut(s) 27, 164, 281, 296, 401, 603
Tru9I TTAA 6 cut(s) 27, 164, 281, 296, 401, 603
TscAI CASTG 1 cut(s) 347
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 2 cut(s) 152, 247
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 2 cut(s) 172, 578
TspRI CASTG 1 cut(s) 347
Tth111I GACNNNGTC 1 cut(s) 131
VpaK11BI GGWCC 1 cut(s) 127
XapI RAATTY 1 cut(s) 90
XbaI TCTAGA 1 cut(s) 484
XmaJI CCTAGG 1 cut(s) 200
XspI CTAG 5 cut(s) 5, 201, 485, 507, 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.