RchiOBHm_Chr2g0123771

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0011022
Physical Location & Seq
Forward (+)
37015081 .. 37021125
6045 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49604

Sequence Viewer

Length: 2112 bp
ATGATGGTCGATTTAGGCATCTCACTTGCAAGATTTGTGACCAGCTCGGACAGTTATGGTCCATTGCTTCTGGGATGGCTGGTCACCGGATCATTTGGTCTTCTAGCTATCATCTTTGCATTTCTTAAATGGGAGAGAAGGACATCTCTGAATTGGGTTAAAGCTGCGGCTAGAGAAAAGAAGAAAGTCTGGAAGAAGCTAAGAGCTCCTCTGTCAACCCATACATGGATAGAAGATATTACTAATGGTGAACAGCCATCTACATGCTGTGTTTGCTTGACTTCCTTGGTGTTTCTTCAGAACCTAAGCACTAAAGCTTCATCGCGCACTTCTGTCCATCGTTGTGTAGTTTGTGGTGTGGCAGCTCATTCTTATTGTTCTGATTTTGCAGTAAAAGATTGCAAGTGTGTGGCACAAGCTGGTTCTACCCATGTTCGACACCACTGGTCAGAAAAATGGGTTAATATGGATGATAATCCTGAGATTTCTGCCTGCTGCTTCTATTGCGATGAGCCATGTGGTGTTCCTTTCCTTGATGCTTCTCCAACATGGCATTGTCTATGGTGTCAGAACCTCATACATGTCAGATGTCACAATAAAATGGCAAAAGAGTCTGGTGATGTTTGTGATCTTGGTACTCTTAGGAGGGTCATTCTCTCTCCTTTATGTGTAAAAGAAATCGGTCACTTAAATGGAGGTGGAGTGCTGCGTTCAATAACAGATGAGTTTATAACTTCATCTGTTCGTGGCCAGTTTAGAAGAAAGCGTCATCGTCCTAAACATGGTAGTGGTCATAACTCATCCAATGATGCTCTGGAGTATCTGTTCAATGGGTTTACTGGTATGAAGAAATCCCGTAGTGAGAAGAGTTTTGACCACTTGAAGGATGGCAGGGCACTGAGCGTTAAGGGTAACCATAATGGCTTTATGCAGAAAAAGGGTGGAATTGTCACCTATGGTCAATATAAGAAATATAAAGTGGTTAATTTACCAAAGGATGCAAGGCCTCTTTTGGTTTTTATAAATGCCAGGAGTGGAGGCCAAAATGGGGCTTCTCTAAGAAGGAGGTTGAACATGCTATTGAATCCTGCACAGGTTTTTGAATTGAGTTCTTCTCAAGGGCCTGAGGTTGGTTTGGAATTGTTCAACGATGTGCAATACTTTAGAGTTTTGGTATGTGGTGGAGATGGCACTGTTGCATGGGTCCTTGATGCCATTGAGAAGCATAAATTTGAATCACCGCCACCTGTTGCTATTATCCCCCTTGGCACAGGAAATGATTTGTCAAGGGTTCTGCAATGGGGAAGAGGCTTCTCTATCGTTGATGGACAAGGTGGCTTAACCACGCTTTTACATGACATTAACCATGCGGCAGTTACAATGTTAGATCGCTGGAAGGTAAACATCAAATCAGAAGATGATCCAAATAAAGTGCAATCCAAGTTTATGATGAACTATTTAGGTATTGGATGTGACGCGAAGGTTGCTTATGAATTTCATGTGATGCGAGAAATAAATCCTGAGAAGTTCAGTAGTCAGTTTGTGAATAAACTGCGATATGCCAAAGAAGGTGCTAGAGATATAATGGACCGAACTTGTGCCGACTTACCATGGCAAGTTTGGCTTGAAGTTGATGGGAAAGACATAGATATTCCAAAGGATTCTGAGGGTCTAATCGTGCTCAACATTGGCAGCTACATGGGTGGAGTGGATCTTTGGCAAAATGATATTGATCATGATGATGACTTTAGTCTTCAGTCAATGCATGATAAAATGCTTGAAGTTGTATGCATATGTGGATCGTGGCACCTTGGCAAACTACAGGTTGGACTATCACAAGCTAGGAGACTAGCCCAGGGGAATATCATAAGAATACATGCATCTAGTCCTTTTCCAGTCCAAATAGATGGGGAGCCATATATACAACAACCTGGCTGTATAGAGATAACACATCATGGGCAGGTGTTTATGTTGAGGAGGGCTTCAGAGGAGCCGAGAGGGCATGCAGCTGCAATCATGACGGAGGTTTTATTAGACGCTGAGTGTAAAGGCATCATTAACGCATCTCAGAAGAAAACACTTCTCCAGCAGATGGCTCTCCAGCTTGCTTGA

Protein Analysis

703

Amino Acids

78.43

Weight (kDa)

8.54

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C1_1 PF00130 147 - 206 1.1e-06 Phorbol esters/diacylglycerol binding domain (C1 domain)
DAGK_cat PF00781 336 - 436 1e-26 Diacylglycerol kinase catalytic domain
DAGK_acc PF00609 482 - 639 1.1e-57 Diacylglycerol kinase accessory domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011022)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63770 AT5G63770
fragaria_vesca FvH4_1g25600 FvH4_1g25600 FvH4_1g25600
malus_domestica MD15G1139400.v1.1
prunus_persica Prupe.4G282200_v2.0.a1 Prupe.4G282200_v2.0.a1 Prupe.4G282200_v2.0.a1
pyrus_communis pycom15g12580
rosa_chinensis RchiOBHm_Chr2g0123771
rosa_laevigata RLG00000018775
rosa_multiflora Rmu_sc0001032.1_g000003
rosa_roxburghii Rroxscaffold_2G00120350
rosa_rugosa Rorug02G0247900
rosa_samantha Rh2AG306800 Rh2BG315300 Rh2CG293200 Rh2DG330500
rosa_wichuraiana Rw2G024620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 731, 1022
AarI CACCTGC 1 cut(s) 1951
Acc36I ACCTGC 1 cut(s) 1951
AccB1I GGYRCC 1 cut(s) 1806
AccB7I CCANNNNNTGG 1 cut(s) 2092
AccII CGCG 2 cut(s) 325, 1478
AciI CCGC 3 cut(s) 167, 1241, 1370
AclWI GGATC 4 cut(s) 97, 1415, 1719, 1807
AcoI YGGCCR 1 cut(s) 748
AcsI RAATTY 2 cut(s) 1229, 1493
AcuI CTGAAG 3 cut(s) 281, 1739, 1968
AfaI GTAC 1 cut(s) 637
AfiI CCNNNNNNNGG 6 cut(s) 225, 782, 883, 1048, 1130, 2092
AflIII ACRYGT 1 cut(s) 580
AjnI CCWGG 3 cut(s) 1028, 1854, 1930
AloI GAACNNNNNNTCC 2 cut(s) 809, 841
Alw21I GWGCWC 2 cut(s) 208, 1683
Alw26I GTCTC 1 cut(s) 1840
AlwI GGATC 4 cut(s) 97, 1415, 1719, 1807
AoxI GGCC 4 cut(s) 748, 1004, 1039, 1121
ApeKI GCWGC 7 cut(s) 164, 362, 495, 706, 1692, 2006, 2009
ApoI RAATTY 2 cut(s) 1229, 1493
AspLEI GCGC 1 cut(s) 327
AspS9I GGNCC 4 cut(s) 59, 1121, 1204, 1588
AsuHPI GGTGA 5 cut(s) 76, 260, 629, 943, 1230
AvaII GGWCC 3 cut(s) 59, 1204, 1588
AxyI CCTNAGG 1 cut(s) 1125
BaeGI GKGCMC 1 cut(s) 898
BalI TGGCCA 1 cut(s) 750
BanI GGYRCC 1 cut(s) 1806
BanII GRGCYC 1 cut(s) 208
BbsI GAAGAC 2 cut(s) 92, 1745
Bbv12I GWGCWC 2 cut(s) 208, 1683
BbvI GCAGC 7 cut(s) 151, 374, 482, 693, 1704, 1996, 2018
BccI CCATC 9 cut(s) 69, 265, 345, 881, 1181, 1319, 1628, 1901, 2086
BciT130I CCWGG 3 cut(s) 1030, 1856, 1932
BclI TGATCA 1 cut(s) 1732
BcoDI GTCTC 1 cut(s) 1840
BfaI CTAG 6 cut(s) 104, 171, 1575, 1842, 1850, 1884
BfmI CTRYAG 1 cut(s) 1820
BfuAI ACCTGC 1 cut(s) 1951
BglI GCCNNNNNGGC 1 cut(s) 1999
BisI GCNGC 9 cut(s) 165, 168, 363, 496, 707, 1371, 1693, 2007, 2010
BlsI GCNGC 9 cut(s) 166, 169, 364, 497, 708, 1372, 1694, 2008, 2011
Bme1390I CCNGG 3 cut(s) 1030, 1856, 1932
Bme18I GGWCC 3 cut(s) 59, 1204, 1588
BmgT120I GGNCC 4 cut(s) 59, 1121, 1204, 1588
BmiI GGNNCC 4 cut(s) 1205, 1808, 1914, 1992
BmrFI CCNGG 3 cut(s) 1030, 1856, 1932
BmsI GCATC 9 cut(s) 27, 526, 799, 988, 1201, 1494, 1889, 2061, 2072
BoxI GACNNNNGTC 1 cut(s) 1749
BpiI GAAGAC 2 cut(s) 92, 1745
BplI GAGNNNNNCTC 2 cut(s) 1099, 1131
BpmI CTGGAG 3 cut(s) 836, 2069, 2084
Bpu10I CCTNAGC 1 cut(s) 305
BpuEI CTTGAG 1 cut(s) 1101
BsaBI GATNNNNATC 2 cut(s) 474, 1731
BsaJI CCNNGG 6 cut(s) 285, 1264, 1610, 1810, 1854, 1855
BsaWI WCCGGW 1 cut(s) 86
BsaXI ACNNNNNCTCC 4 cut(s) 687, 717, 809, 839
Bsc4I CCNNNNNNNGG 6 cut(s) 225, 782, 883, 1048, 1130, 2092
Bse1I ACTGG 4 cut(s) 449, 751, 844, 1895
Bse21I CCTNAGG 1 cut(s) 1125
Bse3DI GCAATG 2 cut(s) 62, 1304
Bse8I GATNNNNATC 2 cut(s) 474, 1731
BseBI CCWGG 3 cut(s) 1030, 1856, 1932
BseDI CCNNGG 6 cut(s) 285, 1264, 1610, 1810, 1854, 1855
BseGI GGATG 6 cut(s) 80, 475, 800, 892, 1003, 1475
BseJI GATNNNNATC 2 cut(s) 474, 1731
BseLI CCNNNNNNNGG 6 cut(s) 225, 782, 883, 1048, 1130, 2092
BseMI GCAATG 2 cut(s) 62, 1304
BseMII CTCAG 7 cut(s) 471, 890, 1116, 1512, 1656, 2031, 2081
BseNI ACTGG 4 cut(s) 449, 751, 844, 1895
BseRI GAGGAG 3 cut(s) 198, 1990, 2003
BseSI GKGCMC 1 cut(s) 898
BseXI GCAGC 7 cut(s) 151, 374, 482, 693, 1704, 1996, 2018
BsgI GTGCAG 1 cut(s) 1074
Bsh1236I CGCG 2 cut(s) 325, 1478
BshFI GGCC 4 cut(s) 750, 1006, 1041, 1123
BshNI GGYRCC 1 cut(s) 1806
BsiHKAI GWGCWC 2 cut(s) 208, 1683
BsiSI CCGG 1 cut(s) 87
BslI CCNNNNNNNGG 6 cut(s) 225, 782, 883, 1048, 1130, 2092
BsmAI GTCTC 1 cut(s) 1840
BsnI GGCC 4 cut(s) 750, 1006, 1041, 1123
Bsp1286I GDGCHC 3 cut(s) 208, 898, 1683
Bsp143I GATC 7 cut(s) 89, 628, 1387, 1420, 1711, 1732, 1799
Bsp19I CCATGG 1 cut(s) 1610
BspACI CCGC 3 cut(s) 167, 1241, 1370
BspANI GGCC 4 cut(s) 750, 1006, 1041, 1123
BspCNI CTCAG 7 cut(s) 472, 891, 1117, 1513, 1657, 2032, 2080
BspFNI CGCG 2 cut(s) 325, 1478
BspHI TCATGA 2 cut(s) 1735, 2016
BspLI GGNNCC 4 cut(s) 1205, 1808, 1914, 1992
BspMI ACCTGC 1 cut(s) 1951
BspPI GGATC 4 cut(s) 97, 1415, 1719, 1807
BspT107I GGYRCC 1 cut(s) 1806
BsrDI GCAATG 2 cut(s) 62, 1304
BsrI ACTGG 4 cut(s) 449, 751, 844, 1895
BssECI CCNNGG 6 cut(s) 285, 1264, 1610, 1810, 1854, 1855
BssMI GATC 7 cut(s) 89, 628, 1387, 1420, 1711, 1732, 1799
BssT1I CCWWGG 4 cut(s) 285, 1264, 1610, 1810
Bst2UI CCWGG 3 cut(s) 1030, 1856, 1932
Bst4CI ACNGT 2 cut(s) 53, 1195
Bst6I CTCTTC 2 cut(s) 860, 1300
BstC8I GCNNGC 3 cut(s) 493, 2004, 2106
BstDSI CCRYGG 1 cut(s) 1610
BstEII GGTNACC 2 cut(s) 82, 911
BstF5I GGATG 6 cut(s) 80, 475, 800, 892, 1003, 1475
BstFNI CGCG 2 cut(s) 325, 1478
BstHHI GCGC 1 cut(s) 327
BstKTI GATC 7 cut(s) 92, 631, 1390, 1423, 1714, 1735, 1802
BstMAI GTCTC 1 cut(s) 1840
BstMBI GATC 7 cut(s) 89, 628, 1387, 1420, 1711, 1732, 1799
BstMWI GCNNNNNNNGC 5 cut(s) 273, 504, 1484, 1621, 1999
BstNI CCWGG 3 cut(s) 1030, 1856, 1932
BstNSI RCATGY 5 cut(s) 267, 584, 1078, 1880, 2006
BstPAI GACNNNNGTC 1 cut(s) 1749
BstPI GGTNACC 2 cut(s) 82, 911
BstSCI CCNGG 3 cut(s) 1028, 1854, 1930
BstSFI CTRYAG 1 cut(s) 1820
BstSLI GKGCMC 1 cut(s) 898
BstUI CGCG 2 cut(s) 325, 1478
BstV1I GCAGC 7 cut(s) 151, 374, 482, 693, 1704, 1996, 2018
BstV2I GAAGAC 2 cut(s) 92, 1745
BstX2I RGATCY 1 cut(s) 1711
BstXI CCANNNNNNTGG 1 cut(s) 1907
BstYI RGATCY 1 cut(s) 1711
Bsu36I CCTNAGG 1 cut(s) 1125
BsuRI GGCC 4 cut(s) 750, 1006, 1041, 1123
BtgI CCRYGG 1 cut(s) 1610
BtgZI GCGATG 2 cut(s) 306, 522
BtsCI GGATG 6 cut(s) 80, 475, 800, 892, 1003, 1475
BtsIMutI CAGTG 3 cut(s) 442, 896, 1191
BveI ACCTGC 1 cut(s) 1951
Cac8I GCNNGC 3 cut(s) 493, 2004, 2106
CciI TCATGA 2 cut(s) 1735, 2016
CfoI GCGC 1 cut(s) 327
Cfr13I GGNCC 4 cut(s) 59, 1121, 1204, 1588
CseI GACGC 3 cut(s) 755, 1484, 2045
Csp6I GTAC 1 cut(s) 636
CviQI GTAC 1 cut(s) 636
DpnI GATC 7 cut(s) 91, 630, 1389, 1422, 1713, 1734, 1801
DpnII GATC 7 cut(s) 89, 628, 1387, 1420, 1711, 1732, 1799
EaeI YGGCCR 1 cut(s) 748
Eam1104I CTCTTC 2 cut(s) 860, 1300
EarI CTCTTC 2 cut(s) 860, 1300
Ecl136II GAGCTC 1 cut(s) 206
Eco130I CCWWGG 4 cut(s) 285, 1264, 1610, 1810
Eco147I AGGCCT 1 cut(s) 1006
Eco24I GRGCYC 1 cut(s) 208
Eco47I GGWCC 3 cut(s) 59, 1204, 1588
Eco53kI GAGCTC 1 cut(s) 206
Eco57I CTGAAG 3 cut(s) 281, 1739, 1968
Eco81I CCTNAGG 1 cut(s) 1125
Eco91I GGTNACC 2 cut(s) 82, 911
EcoICRI GAGCTC 1 cut(s) 206
EcoO109I RGGNCCY 2 cut(s) 1121, 1204
EcoO65I GGTNACC 2 cut(s) 82, 911
EcoRII CCWGG 3 cut(s) 1028, 1854, 1930
EcoT14I CCWWGG 4 cut(s) 285, 1264, 1610, 1810
EcoT22I ATGCAT 3 cut(s) 1767, 1793, 1882
EcoT38I GRGCYC 1 cut(s) 208
ErhI CCWWGG 4 cut(s) 285, 1264, 1610, 1810
FalI AAGNNNNNCTT 2 cut(s) 1608, 1640
FauNDI CATATG 1 cut(s) 1793
FbaI TGATCA 1 cut(s) 1732
Fnu4HI GCNGC 9 cut(s) 165, 168, 363, 496, 707, 1371, 1693, 2007, 2010
FokI GGATG 6 cut(s) 87, 482, 787, 899, 1010, 1482
FriOI GRGCYC 1 cut(s) 208
Fsp4HI GCNGC 9 cut(s) 165, 168, 363, 496, 707, 1371, 1693, 2007, 2010
FspBI CTAG 6 cut(s) 104, 171, 1575, 1842, 1850, 1884
GlaI GCGC 1 cut(s) 326
GluI GCNGC 9 cut(s) 165, 168, 363, 496, 707, 1371, 1693, 2007, 2010
GsuI CTGGAG 3 cut(s) 836, 2069, 2084
HaeIII GGCC 4 cut(s) 750, 1006, 1041, 1123
HapII CCGG 1 cut(s) 87
HgaI GACGC 3 cut(s) 755, 1484, 2045
HhaI GCGC 1 cut(s) 327
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 4 cut(s) 611, 1084, 1235, 1661
HpaII CCGG 1 cut(s) 87
HphI GGTGA 5 cut(s) 76, 260, 629, 943, 1230
Hpy166II GTNNAC 4 cut(s) 216, 251, 837, 1402
Hpy188III TCNNGA 6 cut(s) 190, 479, 815, 1520, 1736, 2017
Hpy8I GTNNAC 4 cut(s) 216, 251, 837, 1402
HpyAV CCTTC 6 cut(s) 132, 877, 1056, 1390, 1474, 1562
HpyCH4III ACNGT 2 cut(s) 53, 1195
HpyF10VI GCNNNNNNNGC 5 cut(s) 273, 504, 1484, 1621, 1999
HspAI GCGC 1 cut(s) 325
Ksp22I TGATCA 1 cut(s) 1732
Kzo9I GATC 7 cut(s) 89, 628, 1387, 1420, 1711, 1732, 1799
LmnI GCTCC 3 cut(s) 211, 1912, 1990
Lsp1109I GCAGC 7 cut(s) 151, 374, 482, 693, 1704, 1996, 2018
LweI GCATC 9 cut(s) 27, 526, 799, 988, 1201, 1494, 1889, 2061, 2072
MaeI CTAG 6 cut(s) 104, 171, 1575, 1842, 1850, 1884
MaeIII GTNAC 8 cut(s) 37, 82, 590, 683, 911, 949, 1375, 1472
MalI GATC 7 cut(s) 91, 630, 1389, 1422, 1713, 1734, 1801
MboI GATC 7 cut(s) 89, 628, 1387, 1420, 1711, 1732, 1799
MflI RGATCY 1 cut(s) 1711
MhlI GDGCHC 3 cut(s) 208, 898, 1683
MlsI TGGCCA 1 cut(s) 750
MluCI AATT 7 cut(s) 151, 945, 985, 1103, 1139, 1229, 1493
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 1 cut(s) 620
MmeI TCCRAC 2 cut(s) 569, 1807
Mox20I TGGCCA 1 cut(s) 750
Mph1103I ATGCAT 3 cut(s) 1767, 1793, 1882
MscI TGGCCA 1 cut(s) 750
MseI TTAA 9 cut(s) 126, 159, 462, 689, 906, 984, 1340, 1362, 2058
MslI CAYNNNNRTG 5 cut(s) 262, 342, 690, 786, 1740
Msp20I TGGCCA 1 cut(s) 750
MspA1I CMGCKG 1 cut(s) 2009
MspI CCGG 1 cut(s) 87
MspR9I CCNGG 3 cut(s) 1030, 1856, 1932
MvaI CCWGG 3 cut(s) 1030, 1856, 1932
MvnI CGCG 2 cut(s) 325, 1478
MwoI GCNNNNNNNGC 5 cut(s) 273, 504, 1484, 1621, 1999
NcoI CCATGG 1 cut(s) 1610
NdeI CATATG 1 cut(s) 1793
NdeII GATC 7 cut(s) 89, 628, 1387, 1420, 1711, 1732, 1799
NlaIV GGNNCC 4 cut(s) 1205, 1808, 1914, 1992
NmeAIII GCCGAG 1 cut(s) 2019
NmuCI GTSAC 6 cut(s) 37, 82, 590, 683, 949, 1472
NsiI ATGCAT 3 cut(s) 1767, 1793, 1882
NspI RCATGY 5 cut(s) 267, 584, 1078, 1880, 2006
PaeI GCATGC 1 cut(s) 2006
PagI TCATGA 2 cut(s) 1735, 2016
PaqCI CACCTGC 1 cut(s) 1951
PasI CCCWGGG 1 cut(s) 1855
PceI AGGCCT 1 cut(s) 1006
PciI ACATGT 1 cut(s) 580
PfeI GAWTC 3 cut(s) 1084, 1235, 1661
PflMI CCANNNNNTGG 1 cut(s) 2092
PkrI GCNGC 9 cut(s) 166, 169, 364, 497, 708, 1372, 1694, 2008, 2011
PleI GAGTC 1 cut(s) 619
PpsI GAGTC 1 cut(s) 619
PpuMI RGGWCCY 1 cut(s) 1204
PscI ACATGT 1 cut(s) 580
PshAI GACNNNNGTC 1 cut(s) 1749
PsiI TTATAA 2 cut(s) 731, 1022
Psp124BI GAGCTC 1 cut(s) 208
Psp5II RGGWCCY 1 cut(s) 1204
Psp6I CCWGG 3 cut(s) 1028, 1854, 1930
PspEI GGTNACC 2 cut(s) 82, 911
PspGI CCWGG 3 cut(s) 1028, 1854, 1930
PspN4I GGNNCC 4 cut(s) 1205, 1808, 1914, 1992
PspPI GGNCC 4 cut(s) 59, 1121, 1204, 1588
PspPPI RGGWCCY 1 cut(s) 1204
PsuI RGATCY 1 cut(s) 1711
PvuII CAGCTG 1 cut(s) 2009
RsaI GTAC 1 cut(s) 637
RsaNI GTAC 1 cut(s) 636
RseI CAYNNNNRTG 5 cut(s) 262, 342, 690, 786, 1740
SacI GAGCTC 1 cut(s) 208
SaqAI TTAA 9 cut(s) 126, 159, 462, 689, 906, 984, 1340, 1362, 2058
SatI GCNGC 9 cut(s) 165, 168, 363, 496, 707, 1371, 1693, 2007, 2010
Sau3AI GATC 7 cut(s) 89, 628, 1387, 1420, 1711, 1732, 1799
Sau96I GGNCC 4 cut(s) 59, 1121, 1204, 1588
SchI GAGTC 1 cut(s) 620
ScrFI CCNGG 3 cut(s) 1030, 1856, 1932
SduI GDGCHC 3 cut(s) 208, 898, 1683
SfaNI GCATC 9 cut(s) 27, 526, 799, 988, 1201, 1494, 1889, 2061, 2072
SfcI CTRYAG 1 cut(s) 1820
SinI GGWCC 3 cut(s) 59, 1204, 1588
SmiMI CAYNNNNRTG 5 cut(s) 262, 342, 690, 786, 1740
SmlI CTYRAG 1 cut(s) 1116
SmoI CTYRAG 1 cut(s) 1116
SphI GCATGC 1 cut(s) 2006
Sse9I AATT 7 cut(s) 151, 945, 985, 1103, 1139, 1229, 1493
SseBI AGGCCT 1 cut(s) 1006
SsiI CCGC 3 cut(s) 167, 1241, 1370
SspMI CTAG 6 cut(s) 104, 171, 1575, 1842, 1850, 1884
SstI GAGCTC 1 cut(s) 208
StuI AGGCCT 1 cut(s) 1006
StyD4I CCNGG 3 cut(s) 1028, 1854, 1930
StyI CCWWGG 4 cut(s) 285, 1264, 1610, 1810
TaaI ACNGT 2 cut(s) 53, 1195
TaqI TCGA 2 cut(s) 9, 436
TaqII GACCGA 2 cut(s) 671, 1605
TasI AATT 7 cut(s) 151, 945, 985, 1103, 1139, 1229, 1493
TauI GCSGC 2 cut(s) 170, 1373
TfiI GAWTC 3 cut(s) 1084, 1235, 1661
Tru1I TTAA 9 cut(s) 126, 159, 462, 689, 906, 984, 1340, 1362, 2058
Tru9I TTAA 9 cut(s) 126, 159, 462, 689, 906, 984, 1340, 1362, 2058
TscAI CASTG 3 cut(s) 449, 903, 1198
TseFI GTSAC 6 cut(s) 37, 82, 590, 683, 949, 1472
TseI GCWGC 7 cut(s) 164, 362, 495, 706, 1692, 2006, 2009
Tsp45I GTSAC 6 cut(s) 37, 82, 590, 683, 949, 1472
TspDTI ATGAA 6 cut(s) 309, 726, 860, 1466, 1487, 1506
TspGWI ACGGA 1 cut(s) 2036
TspRI CASTG 3 cut(s) 449, 903, 1198
Van91I CCANNNNNTGG 1 cut(s) 2092
VpaK11BI GGWCC 3 cut(s) 59, 1204, 1588
XapI RAATTY 2 cut(s) 1229, 1493
XceI RCATGY 5 cut(s) 267, 584, 1078, 1880, 2006
XcmI CCANNNNNNNNNTGG 3 cut(s) 811, 884, 1617
XspI CTAG 6 cut(s) 104, 171, 1575, 1842, 1850, 1884
Zsp2I ATGCAT 3 cut(s) 1767, 1793, 1882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.