FvH4_1g25750

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010330
Physical Location & Seq
Reverse (-)
17670983 .. 17677678
6696 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25750.t2

Sequence Viewer

Length: 1911 bp
ATGGGAGGACTTTGCTCCAAGAGTTCCCACAAGGCGGTCAACGCCTGGGCCGCCGACAATCGCCGCCAAGGGGGTGATGATTACAAGCTGAGAAGCTCCAAGAGGACCACTACTTCTGCTGCTGCTGAGGTGGCTCCGCCGCCGGCCGTGGATATGGACACTGCTCCGCAGCCGCCTAGATACTCCCAAGACGGTCCTGCACTTGTCTACGAAGGAGGTGATGCCACTGATGACTTTTACGATGGGATTCCGCGATATAAATCCGGCTCTTTCCGCAAGGCCAAGGTTTCTGAGGTGGGATCACGGATAGGGAGAGGGGCAGTGTCAGTGTTGGACACGCTTGGGAGCAGCATGACCAATTTGAATTCCGCCACTGGTTTCAATTCAGCGCCGCCAGCCAAGGGGAATGAGATTGCTATTATAGCATTCGAGATTGCCAACACTGTTGTTAAGGGTTACAATCTTATGCAATCTATTTCGACCAGAAGTATTAGGAATTTAAAAGAAGTGGTGTTTCCTTCTGAAGGTGTGCAGTATTTAGTATCCAAAGATACGGATGAGCTTTTGAGGATTGTTGCAGCTGATAAAAGGGAGGAGTTAAAAATTTTCTCGGGGGAGGTGGTTCGTTTTGGAAATCGATGTAAAGATCCTCAGTGGCACAACTTGGACAGATATTTTGAGAAAATCAGCAGAGAACTTATTCCTCAGAAGCATCTGAAGGTGGAGGCAACTTCGGTAATGCAGCAACTGATGACGTTGGTCCAGTATACTGCTGAATTATACCACGAGTTCCATGCATTGGACCGATTCGAACAAGACTATGAACGTAAGCAGTACGAGGAGGATAATTTATCTTCCACTCAAAGAGGTGATACCCTTGGAATCTTGAGATCTGAACTAAAAAGCCAAAGGAAACTAGTTAAAAGTTTAAAGAAAAAGTCACTTTGGTCCAAAAGTATGGAAGAGGTGATGGAAAAGCTTGTTGAAATAGTCCACTTTTTATATCTAGAGATGCATGCAGCCTTTCGTAGTGCTGAAGGTCAGAAAACTGCGGAGGGAGCCACAAATAAATATCAAAAGTTGGGACCTGCTGGACTTTCTTTACATTATGCAAATATTGTTCTCCAAATCGATACTCTTGTAGTCAGGTCAAGTGCTCCGCCGCCAACTACAAGAGATACATTATACCAGAGCTTGCCGCCTAATATAAAATCATCTTTACGCTCCAAATTACATTCTTTCCATATTAAAGAAGAGCTCACTATCACACAAATCAAAGCTGAGATGGAGAAAACTCTGCACTGGCTTGTTCCAATGGCTGCAAATACAGCCAAAGATCACCATGGTTTTGGTTGGGTAGGTGAGTGGGCGAGTACTGGTGCTGAGACGAATCGTAAACCAGCTCCACAGACTGAGGTGATTCGGATCGAGACATTCCATCATGCAAATAGGGAAAAAACAGAAGCCTGTGTACTCAAACTAGTGTTGTGGCTTCATTATTTGGTCTCTCAAAGCAAGCCTGCATCCACTAGTGTGGGTATGCGATCACCTGTTAAGTCCATAGCTACCACAACCCTCCCAAACACAAATAAGCAGCTGACGGACATCCCTCCCTCAGCCGTGCTAACAACTGATGACCAACAGATGCTGCAGGATGTAAGTAAGAGAAAACGGGTTCCCGGAATTAGTAAAAGTCAGGACTTTGAGAATGTTTCTAGTTTGGGAAACCTGGGTAGATTGAACAAGAGCAACAGCTATTCATCAGGGGAAGGAACCAAGAAAGTGTTACCTTTCAGCAGGCTGTCTTCTGGGGTTCCTGTCATTGATTTTGGTATAGATAAGGAAAAGGCATTGGATGTCATAGATAGAGTGGAGACACATAGAGATCATCATCATCATCCAAGGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

637

Amino Acids

71.0

Weight (kDa)

9.09

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 140 - 196 2.1e-21 Domain of unknown function (DUF3475)
DUF668 PF05003 361 - 419 1.1e-18 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08660 AT5G08660 AT5G08660 AT5G08660
fragaria_vesca FvH4_1g25750 FvH4_1g25750
malus_domestica MD15G1140000.v1.1
prunus_persica Prupe.4G285700_v2.0.a1 Prupe.4G285700_v2.0.a1 Prupe.4G285700_v2.0.a1
pyrus_communis pycom15g12650
rosa_chinensis RchiOBHm_Chr3g0484691
rosa_laevigata RLG00000023207
rosa_multiflora Rmu_sc0006192.1_g000003
rosa_roxburghii Rroxscaffold_6G00397520
rosa_rugosa Rorug03G0213500
rosa_samantha Rh3AG263600 Rh3BG298800 Rh3CG296200 Rh3DG292300
rosa_wichuraiana Rw3G023510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1096
AccB7I CCANNNNNTGG 1 cut(s) 799
AccI GTMKAC 2 cut(s) 207, 767
AccII CGCG 1 cut(s) 253
AclWI GGATC 3 cut(s) 307, 641, 1433
AcoI YGGCCR 1 cut(s) 144
AcsI RAATTY 3 cut(s) 364, 496, 603
AcuI CTGAAG 3 cut(s) 543, 737, 1056
AfaI GTAC 3 cut(s) 836, 1375, 1473
AfiI CCNNNNNNNGG 5 cut(s) 34, 70, 401, 524, 799
AgsI TTSAA 4 cut(s) 364, 382, 986, 1741
AhlI ACTAGT 3 cut(s) 916, 1480, 1529
AjnI CCWGG 2 cut(s) 44, 1728
AleI CACNNNNGTG 1 cut(s) 1532
Alw21I GWGCWC 2 cut(s) 1159, 1260
Alw26I GTCTC 4 cut(s) 1379, 1424, 1510, 1868
AlwI GGATC 3 cut(s) 307, 641, 1433
AlwNI CAGNNNCTG 2 cut(s) 748, 1648
Ama87I CYCGRG 1 cut(s) 610
AoxI GGCC 3 cut(s) 48, 144, 279
ApoI RAATTY 3 cut(s) 364, 496, 603
ArsI GACNNNNNNTTYG 2 cut(s) 659, 691
Asp700I GAANNNNTTC 1 cut(s) 699
AspLEI GCGC 1 cut(s) 391
AspS9I GGNCC 7 cut(s) 48, 105, 194, 760, 802, 948, 1085
AsuC2I CCSGG 1 cut(s) 1680
AsuHPI GGTGA 8 cut(s) 86, 230, 881, 979, 1331, 1373, 1429, 1539
AsuII TTCGAA 1 cut(s) 810
AvaI CYCGRG 1 cut(s) 610
AvaII GGWCC 6 cut(s) 105, 194, 760, 802, 948, 1085
BaeI ACNNNNGTAYC 2 cut(s) 1125, 1158
BanII GRGCYC 1 cut(s) 1260
BarI GAAGNNNNNNTAC 2 cut(s) 1455, 1487
BauI CACGAG 1 cut(s) 785
BbsI GAAGAC 1 cut(s) 1797
Bbv12I GWGCWC 2 cut(s) 1159, 1260
BbvCI CCTCAGC 2 cut(s) 126, 1615
BccI CCATC 4 cut(s) 236, 964, 1279, 1446
BceAI ACGGC 2 cut(s) 131, 1604
BcgI CGANNNNNNTGC 2 cut(s) 776, 810
BciT130I CCWGG 2 cut(s) 46, 1730
BciVI GTATCC 1 cut(s) 553
BcnI CCSGG 1 cut(s) 1680
BcoDI GTCTC 4 cut(s) 1379, 1424, 1510, 1868
BcuI ACTAGT 3 cut(s) 916, 1480, 1529
BfaI CTAG 6 cut(s) 177, 917, 1007, 1481, 1530, 1716
BfmI CTRYAG 1 cut(s) 1649
BfoI RGCGCY 1 cut(s) 392
BfuAI ACCTGC 1 cut(s) 1096
BfuI GTATCC 1 cut(s) 553
BglII AGATCT 1 cut(s) 890
BmcAI AGTACT 1 cut(s) 1375
Bme1390I CCNGG 3 cut(s) 46, 1680, 1730
Bme18I GGWCC 6 cut(s) 105, 194, 760, 802, 948, 1085
BmeT110I CYCGRG 1 cut(s) 610
BmgT120I GGNCC 7 cut(s) 48, 105, 194, 760, 802, 948, 1085
BmiI GGNNCC 6 cut(s) 135, 1060, 1086, 1677, 1774, 1815
BmrFI CCNGG 3 cut(s) 46, 1680, 1730
BmsI GCATC 5 cut(s) 211, 721, 1002, 1532, 1635
BoxI GACNNNNGTC 1 cut(s) 758
BpiI GAAGAC 1 cut(s) 1797
Bpu10I CCTNAGC 2 cut(s) 126, 1615
Bpu14I TTCGAA 1 cut(s) 810
BpuEI CTTGAG 1 cut(s) 907
BpuMI CCSGG 1 cut(s) 1680
Bsa29I ATCGAT 2 cut(s) 637, 1131
BsaBI GATNNNNATC 3 cut(s) 259, 1424, 1890
BsaI GGTCTC 1 cut(s) 1510
BsaJI CCNNGG 9 cut(s) 45, 67, 147, 282, 399, 877, 1342, 1729, 1901
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 70, 401, 524, 799
Bse118I RCCGGY 1 cut(s) 142
Bse1I ACTGG 4 cut(s) 379, 763, 1307, 1381
Bse8I GATNNNNATC 3 cut(s) 259, 1424, 1890
BseBI CCWGG 2 cut(s) 46, 1730
BseCI ATCGAT 2 cut(s) 637, 1131
BseDI CCNNGG 9 cut(s) 45, 67, 147, 282, 399, 877, 1342, 1729, 1901
BseGI GGATG 6 cut(s) 562, 1523, 1605, 1660, 1861, 1897
BseJI GATNNNNATC 3 cut(s) 259, 1424, 1890
BseLI CCNNNNNNNGG 5 cut(s) 34, 70, 401, 524, 799
BseMII CTCAG 9 cut(s) 80, 117, 282, 665, 719, 1272, 1374, 1404, 1629
BseNI ACTGG 4 cut(s) 379, 763, 1307, 1381
BseRI GAGGAG 2 cut(s) 608, 854
BseX3I CGGCCG 1 cut(s) 144
BsgI GTGCAG 3 cut(s) 183, 551, 1283
Bsh1236I CGCG 1 cut(s) 253
Bsh1285I CGRYCG 1 cut(s) 147
BshFI GGCC 3 cut(s) 50, 146, 281
BshVI ATCGAT 2 cut(s) 637, 1131
BsiEI CGRYCG 1 cut(s) 147
BsiHKAI GWGCWC 2 cut(s) 1159, 1260
BsiHKCI CYCGRG 1 cut(s) 610
BsiSI CCGG 3 cut(s) 143, 264, 1680
BslFI GGGAC 1 cut(s) 1098
BslI CCNNNNNNNGG 5 cut(s) 34, 70, 401, 524, 799
BsmAI GTCTC 4 cut(s) 1379, 1424, 1510, 1868
BsmBI CGTCTC 1 cut(s) 1379
BsmFI GGGAC 1 cut(s) 1098
BsmI GAATGC 1 cut(s) 425
BsnI GGCC 3 cut(s) 50, 146, 281
Bso31I GGTCTC 1 cut(s) 1510
BsoBI CYCGRG 1 cut(s) 610
Bsp119I TTCGAA 1 cut(s) 810
Bsp1286I GDGCHC 2 cut(s) 1159, 1260
Bsp143I GATC 7 cut(s) 299, 646, 890, 1336, 1425, 1544, 1885
Bsp19I CCATGG 1 cut(s) 1342
BspANI GGCC 3 cut(s) 50, 146, 281
BspCNI CTCAG 9 cut(s) 81, 118, 283, 664, 718, 1273, 1375, 1405, 1628
BspDI ATCGAT 2 cut(s) 637, 1131
BspFNI CGCG 1 cut(s) 253
BspLI GGNNCC 6 cut(s) 135, 1060, 1086, 1677, 1774, 1815
BspMAI CTGCAG 1 cut(s) 1653
BspMI ACCTGC 1 cut(s) 1096
BspPI GGATC 3 cut(s) 307, 641, 1433
BspQI GCTCTTC 1 cut(s) 1248
BspT104I TTCGAA 1 cut(s) 810
BspTNI GGTCTC 1 cut(s) 1510
BsrFI RCCGGY 1 cut(s) 142
BsrI ACTGG 4 cut(s) 379, 763, 1307, 1381
BssAI RCCGGY 1 cut(s) 142
BssECI CCNNGG 9 cut(s) 45, 67, 147, 282, 399, 877, 1342, 1729, 1901
BssMI GATC 7 cut(s) 299, 646, 890, 1336, 1425, 1544, 1885
BssNAI GTATAC 1 cut(s) 768
BssSI CACGAG 1 cut(s) 785
BssT1I CCWWGG 6 cut(s) 67, 282, 399, 877, 1342, 1901
Bst1107I GTATAC 1 cut(s) 768
Bst2BI CACGAG 1 cut(s) 785
Bst2UI CCWGG 2 cut(s) 46, 1730
Bst4CI ACNGT 2 cut(s) 194, 445
Bst6I CTCTTC 2 cut(s) 957, 1248
BstBI TTCGAA 1 cut(s) 810
BstC8I GCNNGC 7 cut(s) 144, 396, 1017, 1196, 1517, 1521, 1799
BstDEI CTNAG 9 cut(s) 89, 126, 291, 651, 705, 1281, 1383, 1413, 1615
BstDSI CCRYGG 2 cut(s) 147, 1342
BstENI CCTNNNNNAGG 1 cut(s) 522
BstF5I GGATG 6 cut(s) 562, 1523, 1605, 1660, 1861, 1897
BstFNI CGCG 1 cut(s) 253
BstH2I RGCGCY 1 cut(s) 392
BstHHI GCGC 1 cut(s) 391
BstKTI GATC 7 cut(s) 302, 649, 893, 1339, 1428, 1547, 1888
BstMAI GTCTC 4 cut(s) 1379, 1424, 1510, 1868
BstMBI GATC 7 cut(s) 299, 646, 890, 1336, 1425, 1544, 1885
BstMCI CGRYCG 1 cut(s) 147
BstMWI GCNNNNNNNGC 8 cut(s) 41, 50, 131, 273, 395, 422, 1058, 1328
BstNI CCWGG 2 cut(s) 46, 1730
BstNSI RCATGY 1 cut(s) 1019
BstPAI GACNNNNGTC 1 cut(s) 758
BstSCI CCNGG 3 cut(s) 44, 1678, 1728
BstSFI CTRYAG 1 cut(s) 1649
BstUI CGCG 1 cut(s) 253
BstV2I GAAGAC 1 cut(s) 1797
BstX2I RGATCY 2 cut(s) 646, 890
BstXI CCANNNNNNTGG 3 cut(s) 958, 1349, 1534
BstYI RGATCY 2 cut(s) 646, 890
BstZ17I GTATAC 1 cut(s) 768
BstZI CGGCCG 1 cut(s) 144
Bsu15I ATCGAT 2 cut(s) 637, 1131
BsuI GTATCC 1 cut(s) 553
BsuRI GGCC 3 cut(s) 50, 146, 281
BsuTUI ATCGAT 2 cut(s) 637, 1131
BtgI CCRYGG 2 cut(s) 147, 1342
BtsCI GGATG 6 cut(s) 562, 1523, 1605, 1660, 1861, 1897
BtsI GCAGTG 2 cut(s) 159, 327
BtsIMutI CAGTG 8 cut(s) 159, 225, 327, 333, 372, 441, 659, 1300
BveI ACCTGC 1 cut(s) 1096
Cac8I GCNNGC 7 cut(s) 144, 396, 1017, 1196, 1517, 1521, 1799
CaiI CAGNNNCTG 2 cut(s) 748, 1648
CfoI GCGC 1 cut(s) 391
Cfr10I RCCGGY 1 cut(s) 142
Cfr13I GGNCC 7 cut(s) 48, 105, 194, 760, 802, 948, 1085
ClaI ATCGAT 2 cut(s) 637, 1131
Csp6I GTAC 3 cut(s) 835, 1374, 1472
CviAII CATG 5 cut(s) 352, 794, 1016, 1343, 1442
CviQI GTAC 3 cut(s) 835, 1374, 1472
DdeI CTNAG 9 cut(s) 89, 126, 291, 651, 705, 1281, 1383, 1413, 1615
DpnI GATC 7 cut(s) 301, 648, 892, 1338, 1427, 1546, 1887
DpnII GATC 7 cut(s) 299, 646, 890, 1336, 1425, 1544, 1885
DraI TTTAAA 2 cut(s) 501, 930
EaeI YGGCCR 1 cut(s) 144
EagI CGGCCG 1 cut(s) 144
Eam1104I CTCTTC 2 cut(s) 957, 1248
EarI CTCTTC 2 cut(s) 957, 1248
EciI GGCGGA 3 cut(s) 126, 358, 1149
Ecl136II GAGCTC 1 cut(s) 1258
EclXI CGGCCG 1 cut(s) 144
Eco130I CCWWGG 6 cut(s) 67, 282, 399, 877, 1342, 1901
Eco24I GRGCYC 1 cut(s) 1260
Eco31I GGTCTC 1 cut(s) 1510
Eco47I GGWCC 6 cut(s) 105, 194, 760, 802, 948, 1085
Eco52I CGGCCG 1 cut(s) 144
Eco53kI GAGCTC 1 cut(s) 1258
Eco57I CTGAAG 3 cut(s) 543, 737, 1056
Eco88I CYCGRG 1 cut(s) 610
EcoICRI GAGCTC 1 cut(s) 1258
EcoNI CCTNNNNNAGG 1 cut(s) 522
EcoO109I RGGNCCY 1 cut(s) 1085
EcoRI GAATTC 1 cut(s) 364
EcoRII CCWGG 2 cut(s) 44, 1728
EcoT14I CCWWGG 6 cut(s) 67, 282, 399, 877, 1342, 1901
EcoT22I ATGCAT 2 cut(s) 799, 1017
EcoT38I GRGCYC 1 cut(s) 1260
ErhI CCWWGG 6 cut(s) 67, 282, 399, 877, 1342, 1901
Esp3I CGTCTC 1 cut(s) 1379
FaeI CATG 5 cut(s) 355, 797, 1019, 1346, 1445
FaqI GGGAC 1 cut(s) 1098
FatI CATG 5 cut(s) 351, 793, 1015, 1342, 1441
FblI GTMKAC 2 cut(s) 207, 767
FokI GGATG 6 cut(s) 569, 1510, 1592, 1667, 1868, 1884
FriOI GRGCYC 1 cut(s) 1260
FspBI CTAG 6 cut(s) 177, 917, 1007, 1481, 1530, 1716
GlaI GCGC 1 cut(s) 390
HaeII RGCGCY 1 cut(s) 392
HaeIII GGCC 3 cut(s) 50, 146, 281
HapII CCGG 3 cut(s) 143, 264, 1680
HhaI GCGC 1 cut(s) 391
Hin1II CATG 5 cut(s) 355, 797, 1019, 1346, 1445
Hin6I GCGC 1 cut(s) 389
HinP1I GCGC 1 cut(s) 389
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 977
HinfI GANTC 5 cut(s) 247, 807, 882, 1390, 1420
HpaII CCGG 3 cut(s) 143, 264, 1680
HphI GGTGA 8 cut(s) 86, 230, 881, 979, 1331, 1373, 1429, 1539
Hpy166II GTNNAC 6 cut(s) 40, 208, 768, 994, 1397, 1472
Hpy188I TCNGA 7 cut(s) 292, 523, 708, 717, 895, 1044, 1425
Hpy188III TCNNGA 5 cut(s) 430, 886, 1007, 1429, 1697
Hpy8I GTNNAC 6 cut(s) 40, 208, 768, 994, 1397, 1472
HpyAV CCTTC 6 cut(s) 206, 518, 528, 712, 1031, 1763
HpyCH4III ACNGT 2 cut(s) 194, 445
HpyCH4IV ACGT 2 cut(s) 755, 826
HpyF10VI GCNNNNNNNGC 8 cut(s) 41, 50, 131, 273, 395, 422, 1058, 1328
HpyF3I CTNAG 9 cut(s) 89, 126, 291, 651, 705, 1281, 1383, 1413, 1615
HpySE526I ACGT 2 cut(s) 755, 826
Hsp92II CATG 5 cut(s) 355, 797, 1019, 1346, 1445
HspAI GCGC 1 cut(s) 389
KroI GCCGGC 1 cut(s) 142
KroNI GCCGGC 1 cut(s) 144
Kzo9I GATC 7 cut(s) 299, 646, 890, 1336, 1425, 1544, 1885
LguI GCTCTTC 1 cut(s) 1248
LmnI GCTCC 9 cut(s) 20, 101, 139, 169, 345, 1058, 1162, 1229, 1408
LweI GCATC 5 cut(s) 211, 721, 1002, 1532, 1635
MaeI CTAG 6 cut(s) 177, 917, 1007, 1481, 1530, 1716
MaeII ACGT 2 cut(s) 755, 826
MaeIII GTNAC 3 cut(s) 455, 939, 1785
MalI GATC 7 cut(s) 301, 648, 892, 1338, 1427, 1546, 1887
MboI GATC 7 cut(s) 299, 646, 890, 1336, 1425, 1544, 1885
MboII GAAGA 4 cut(s) 846, 974, 1265, 1797
MflI RGATCY 2 cut(s) 646, 890
MhlI GDGCHC 2 cut(s) 1159, 1260
MluCI AATT 9 cut(s) 358, 364, 382, 496, 603, 776, 847, 1229, 1683
MmeI TCCRAC 1 cut(s) 312
Mph1103I ATGCAT 2 cut(s) 799, 1017
MroNI GCCGGC 1 cut(s) 142
MroXI GAANNNNTTC 1 cut(s) 699
MseI TTAA 7 cut(s) 450, 500, 599, 921, 929, 1248, 1554
MslI CAYNNNNRTG 1 cut(s) 1532
MspA1I CMGCKG 2 cut(s) 581, 1597
MspI CCGG 3 cut(s) 143, 264, 1680
MspR9I CCNGG 3 cut(s) 46, 1680, 1730
Mva1269I GAATGC 1 cut(s) 425
MvaI CCWGG 2 cut(s) 46, 1730
MvnI CGCG 1 cut(s) 253
MwoI GCNNNNNNNGC 8 cut(s) 41, 50, 131, 273, 395, 422, 1058, 1328
NaeI GCCGGC 1 cut(s) 144
NciI CCSGG 1 cut(s) 1680
NcoI CCATGG 1 cut(s) 1342
NdeII GATC 7 cut(s) 299, 646, 890, 1336, 1425, 1544, 1885
NgoMIV GCCGGC 1 cut(s) 142
NlaIII CATG 5 cut(s) 355, 797, 1019, 1346, 1445
NlaIV GGNNCC 6 cut(s) 135, 1060, 1086, 1677, 1774, 1815
NmuCI GTSAC 1 cut(s) 939
NsiI ATGCAT 2 cut(s) 799, 1017
NspI RCATGY 1 cut(s) 1019
NspV TTCGAA 1 cut(s) 810
OliI CACNNNNGTG 1 cut(s) 1532
PaeI GCATGC 1 cut(s) 1019
PciSI GCTCTTC 1 cut(s) 1248
PctI GAATGC 1 cut(s) 425
PdiI GCCGGC 1 cut(s) 144
PdmI GAANNNNTTC 1 cut(s) 699
PfeI GAWTC 5 cut(s) 247, 807, 882, 1390, 1420
PflMI CCANNNNNTGG 1 cut(s) 799
PfoI TCCNGGA 1 cut(s) 1678
PpuMI RGGWCCY 1 cut(s) 1085
PshAI GACNNNNGTC 1 cut(s) 758
Psp124BI GAGCTC 1 cut(s) 1260
Psp5II RGGWCCY 1 cut(s) 1085
Psp6I CCWGG 2 cut(s) 44, 1728
PspGI CCWGG 2 cut(s) 44, 1728
PspN4I GGNNCC 6 cut(s) 135, 1060, 1086, 1677, 1774, 1815
PspPI GGNCC 7 cut(s) 48, 105, 194, 760, 802, 948, 1085
PspPPI RGGWCCY 1 cut(s) 1085
PstI CTGCAG 1 cut(s) 1653
PstNI CAGNNNCTG 2 cut(s) 748, 1648
PsuI RGATCY 2 cut(s) 646, 890
PvuII CAGCTG 2 cut(s) 581, 1597
RsaI GTAC 3 cut(s) 836, 1375, 1473
RsaNI GTAC 3 cut(s) 835, 1374, 1472
RseI CAYNNNNRTG 1 cut(s) 1532
SacI GAGCTC 1 cut(s) 1260
SapI GCTCTTC 1 cut(s) 1248
SaqAI TTAA 7 cut(s) 450, 500, 599, 921, 929, 1248, 1554
Sau3AI GATC 7 cut(s) 299, 646, 890, 1336, 1425, 1544, 1885
Sau96I GGNCC 7 cut(s) 48, 105, 194, 760, 802, 948, 1085
ScaI AGTACT 1 cut(s) 1375
ScrFI CCNGG 3 cut(s) 46, 1680, 1730
SduI GDGCHC 2 cut(s) 1159, 1260
SfaNI GCATC 5 cut(s) 211, 721, 1002, 1532, 1635
SfcI CTRYAG 1 cut(s) 1649
SfuI TTCGAA 1 cut(s) 810
SinI GGWCC 6 cut(s) 105, 194, 760, 802, 948, 1085
SmiMI CAYNNNNRTG 1 cut(s) 1532
SmlI CTYRAG 1 cut(s) 886
SmoI CTYRAG 1 cut(s) 886
SpeI ACTAGT 3 cut(s) 916, 1480, 1529
SphI GCATGC 1 cut(s) 1019
Sse9I AATT 9 cut(s) 358, 364, 382, 496, 603, 776, 847, 1229, 1683
SspI AATATT 1 cut(s) 1117
SspMI CTAG 6 cut(s) 177, 917, 1007, 1481, 1530, 1716
SstI GAGCTC 1 cut(s) 1260
StyD4I CCNGG 3 cut(s) 44, 1678, 1728
StyI CCWWGG 6 cut(s) 67, 282, 399, 877, 1342, 1901
TaaI ACNGT 2 cut(s) 194, 445
TaiI ACGT 2 cut(s) 758, 829
TaqI TCGA 6 cut(s) 429, 479, 637, 810, 1131, 1428
TaqII GACCGA 1 cut(s) 819
TasI AATT 9 cut(s) 358, 364, 382, 496, 603, 776, 847, 1229, 1683
TatI WGTACW 2 cut(s) 1373, 1471
TauI GCSGC 7 cut(s) 53, 66, 142, 175, 394, 1165, 1201
TfiI GAWTC 5 cut(s) 247, 807, 882, 1390, 1420
Tru1I TTAA 7 cut(s) 450, 500, 599, 921, 929, 1248, 1554
Tru9I TTAA 7 cut(s) 450, 500, 599, 921, 929, 1248, 1554
TscAI CASTG 8 cut(s) 166, 232, 327, 333, 379, 448, 659, 1307
TseFI GTSAC 1 cut(s) 939
Tsp45I GTSAC 1 cut(s) 939
TspDTI ATGAA 3 cut(s) 837, 1484, 1749
TspGWI ACGGA 3 cut(s) 319, 569, 1616
TspRI CASTG 8 cut(s) 166, 232, 327, 333, 379, 448, 659, 1307
Van91I CCANNNNNTGG 1 cut(s) 799
VpaK11BI GGWCC 6 cut(s) 105, 194, 760, 802, 948, 1085
XagI CCTNNNNNAGG 1 cut(s) 522
XapI RAATTY 3 cut(s) 364, 496, 603
XbaI TCTAGA 1 cut(s) 1006
XceI RCATGY 1 cut(s) 1019
XmiI GTMKAC 2 cut(s) 207, 767
XmnI GAANNNNTTC 1 cut(s) 699
XspI CTAG 6 cut(s) 177, 917, 1007, 1481, 1530, 1716
ZrmI AGTACT 1 cut(s) 1375
Zsp2I ATGCAT 2 cut(s) 799, 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.