MD15G1140000.v1.1

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010330
Physical Location & Seq
Reverse (-)
10291217 .. 10300162
8946 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1140000.v1.1.491

Sequence Viewer

Length: 1929 bp
ATGGGAGCGGTTTGTTCCAAGAAGTCGAGGAGTACGAGCGTGAAGAGCGCCGGTTGGGATATCAATCGGCAACAAGTTCAAGTTGAGCAGCCCAAGTCAAGCTTGAAGACGGGTGGCGGCTTCACTCCGTCCTCGCCTCAGCCTCCGCGCAGGGATGACAACGAGGAACAGTTTTCAGACAAGGAAGACAATCAGATTCAGATGGATAGAATTAATCCTTTTGCGTATGAGTCTGACGATGAATTCTACGACGGAATTCCGCGCTATCCCACCTCCTTGTCTCAGAAATCCAGCTCCTTTCGCTCTAAGCAGGCCGCCGCTAAGGTTTCTGAGGTGAGTTCGCGGTTGGGCAGAGCTGGCAGTGCTGGCCTTGGAAAGGCAGTCCAGGTCTTGGACACACTCGGGAGCAGCATGACAAATTTGAATTCCGGCCCTGGTTTCGCTTCCGCAGCACCTGCCAAAGGCAATGAAATTGCAATCATTTCGTTTGAGATTGCCAACACTATTGTTAAGGGTTCCAATCTAATGCAATCCCTTTCCAAGCGATCTATGCGCCAGTTGAAAGAAGTGGTGCTTGTTTCACAAGGTGTGCAGCATTTGGTGTCCAAAGATATGGATGAATTATTAAGGATTGTTGGAGCTGACAAAAGGGAAGAACTACAAATTTTTTCGGGGGAGGTTGTTCGTTTTGGAAATCAATGCAAGGAGTCTCAGTGGCACAACTTGGACCGATATTTTGACAAAATCAGCAGAGAACTTACCCCTCTGAAGCATCTGAAGGAGGATGTAGGTTCGTTGATGCAGCAACTGATGGCTTTGGTCTTGTTTACTGCTGAATTATACCATGAGTTTCATGCGTTGGACCGATTGGAACAAATCTATGCACGCAAGCGTCAGGAAGAAGGTGACAGCCTTGGAAGCTTGAGGTCAGGATTAAAAAGCCAAAGGAAAATAGTTAAATCTTTAAAGAAAAAGTCGCTTTGGTCCAAAAGTATGGAAGAGGTGATGGAGAAGCTTGTTGATATTGTCCACTTTTTACATCTAGAAATACATTCGGCCTTTGGTGGTGCTGATAGTCATGAAGCAGTGGAGGGACCTATGAGCAATCATCAAAGACTCGGACCAGCTGGACTTTCTTTACACTATGCAAATATAATCCTCCAAATTGATAGTCTTGTGAGTGTAGCCCGGTCAAGTTCTATGCCTCAAAATACAAAGGATACATTATATCAGAACTTGCCGCCTAATATAAAATCATCCTTGCGCTCCAAATTACAGTCTTTTCATGTTAAAGAAGAGCTTAGTATTACAGATATCAAAGCTGAGATGGAGAAAACTCTACACTGGCTTGTTCCAGTTGCTACAAACACAGCAAAAGCCCATCATGGTTTTGGCTGGGTAGGAGAGTGGGCGGGCTCTGGTTCCGAGTCAAACCGAAAGCCAATATCGCAGACTGAAGCGATTCGAATTGAGACATTCCATCATGCAGACAAGGAAAAAACAGAAGCCTGTATACTCGATCTAATCTTATGGCTTCACCATTTGGCTTGTCAAACCAAGTCTAGTGCCGATAAGGGGGGCATGAAGTCACCTGTCAAGTCCACAACCAGTACAACCCTCGTGAAGACAAATAAGCAGCTGCCCACTGGAGTTCAACCAAGCATGCTAACAACTGAAGACGCGGAGATGCTGCAGGATGTGAGCAAGAGAAAACGGATTCCAGGTAATAGTAAGAGTCAGGACTTTGACAGTGCCAGCTCTAGTTCAACAGAGGGCCGAAAATTGAGTAAGAGTAACAGCTACTCACATGGGCAAGGAAGCGAGAAATTGTTACATTTGAGTAGGCCCTCGTCTGGCGTTGATTTTGGTATCGATAAGGAAAAAGCACTGGATGTTATAGATAGAGTGGACGGACTCACAATCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

643

Amino Acids

70.96

Weight (kDa)

8.78

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 160 - 216 1.6e-20 Domain of unknown function (DUF3475)
DUF668 PF05003 372 - 459 1.1e-30 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08660 AT5G08660 AT5G08660 AT5G08660
fragaria_vesca FvH4_1g25750 FvH4_1g25750
malus_domestica MD15G1140000.v1.1
prunus_persica Prupe.4G285700_v2.0.a1 Prupe.4G285700_v2.0.a1 Prupe.4G285700_v2.0.a1
pyrus_communis pycom15g12650
rosa_chinensis RchiOBHm_Chr3g0484691
rosa_laevigata RLG00000023207
rosa_multiflora Rmu_sc0006192.1_g000003
rosa_roxburghii Rroxscaffold_6G00397520
rosa_rugosa Rorug03G0213500
rosa_samantha Rh3AG263600 Rh3BG298800 Rh3CG296200 Rh3DG292300
rosa_wichuraiana Rw3G023510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 463
Acc36I ACCTGC 1 cut(s) 463
AccB7I CCANNNNNTGG 1 cut(s) 391
AccBSI CCGCTC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 1513
AccII CGCG 4 cut(s) 148, 262, 343, 1682
AcsI RAATTY 5 cut(s) 242, 255, 418, 424, 663
AcuI CTGAAG 4 cut(s) 788, 797, 1476, 1695
AdeI CACNNNGTG 1 cut(s) 587
AfaI GTAC 2 cut(s) 34, 1612
AfiI CCNNNNNNNGG 5 cut(s) 376, 391, 461, 1575, 1853
AgsI TTSAA 6 cut(s) 80, 106, 424, 562, 1655, 1767
AjnI CCWGG 3 cut(s) 384, 433, 1720
Alw26I GTCTC 3 cut(s) 285, 714, 1466
AlwNI CAGNNNCTG 2 cut(s) 455, 808
Ama87I CYCGRG 1 cut(s) 401
AoxI GGCC 6 cut(s) 312, 367, 430, 1056, 1774, 1844
ApeKI GCWGC 8 cut(s) 88, 408, 449, 592, 802, 1636, 1639, 1690
ApoI RAATTY 5 cut(s) 242, 255, 418, 424, 663
ArsI GACNNNNNNTTYG 4 cut(s) 719, 751, 1156, 1188
AseI ATTAAT 1 cut(s) 213
Asp700I GAANNNNTTC 1 cut(s) 1461
AspLEI GCGC 5 cut(s) 50, 150, 264, 555, 1266
AspS9I GGNCC 8 cut(s) 431, 727, 862, 984, 1094, 1121, 1774, 1845
AsuC2I CCSGG 1 cut(s) 1189
AsuHPI GGTGA 5 cut(s) 346, 917, 1015, 1529, 1581
AsuII TTCGAA 1 cut(s) 1465
AvaI CYCGRG 1 cut(s) 401
AvaII GGWCC 5 cut(s) 727, 862, 984, 1094, 1121
BanII GRGCYC 1 cut(s) 1418
BarI GAAGNNNNNNTAC 2 cut(s) 1497, 1529
BauI CACGAG 1 cut(s) 1619
BbsI GAAGAC 4 cut(s) 113, 192, 1631, 1683
BbvCI CCTCAGC 1 cut(s) 138
BbvI GCAGC 8 cut(s) 100, 420, 461, 604, 814, 1626, 1648, 1677
BccI CCATC 6 cut(s) 196, 805, 1000, 1321, 1389, 1488
BcgI CGANNNNNNTGC 2 cut(s) 465, 499
BciT130I CCWGG 3 cut(s) 386, 435, 1722
BciVI GTATCC 1 cut(s) 1213
BcnI CCSGG 1 cut(s) 1189
BcoDI GTCTC 3 cut(s) 285, 714, 1466
BfaI CTAG 3 cut(s) 1043, 1563, 1761
BfmI CTRYAG 1 cut(s) 1691
BfoI RGCGCY 1 cut(s) 51
BfuAI ACCTGC 1 cut(s) 463
BfuI GTATCC 1 cut(s) 1213
Bme1390I CCNGG 4 cut(s) 386, 435, 1189, 1722
Bme18I GGWCC 5 cut(s) 727, 862, 984, 1094, 1121
BmeT110I CYCGRG 1 cut(s) 401
BmgT120I GGNCC 8 cut(s) 431, 727, 862, 984, 1094, 1121, 1774, 1845
BmiI GGNNCC 3 cut(s) 517, 1095, 1423
BmrFI CCNGG 4 cut(s) 386, 435, 1189, 1722
BmsI GCATC 3 cut(s) 781, 789, 1677
BpiI GAAGAC 4 cut(s) 113, 192, 1631, 1683
BpmI CTGGAG 1 cut(s) 1668
Bpu10I CCTNAGC 2 cut(s) 138, 321
Bpu14I TTCGAA 1 cut(s) 1465
BpuEI CTTGAG 1 cut(s) 943
BpuMI CCSGG 1 cut(s) 1189
Bsa29I ATCGAT 1 cut(s) 1872
BsaBI GATNNNNATC 1 cut(s) 63
BsaJI CCNNGG 3 cut(s) 370, 433, 913
BsaXI ACNNNNNCTCC 2 cut(s) 698, 728
Bsc4I CCNNNNNNNGG 5 cut(s) 376, 391, 461, 1575, 1853
Bse118I RCCGGY 1 cut(s) 50
Bse1I ACTGG 6 cut(s) 556, 1349, 1355, 1608, 1651, 1893
Bse3DI GCAATG 1 cut(s) 472
Bse8I GATNNNNATC 1 cut(s) 63
BseBI CCWGG 3 cut(s) 386, 435, 1722
BseCI ATCGAT 1 cut(s) 1872
BseDI CCNNGG 3 cut(s) 370, 433, 913
BseGI GGATG 6 cut(s) 160, 622, 790, 1256, 1702, 1897
BseJI GATNNNNATC 1 cut(s) 63
BseLI CCNNNNNNNGG 5 cut(s) 376, 391, 461, 1575, 1853
BseMI GCAATG 1 cut(s) 472
BseMII CTCAG 5 cut(s) 152, 296, 321, 725, 1314
BseNI ACTGG 6 cut(s) 556, 1349, 1355, 1608, 1651, 1893
BseRI GAGGAG 1 cut(s) 43
BseXI GCAGC 8 cut(s) 100, 420, 461, 604, 814, 1626, 1648, 1677
BseYI CCCAGC 1 cut(s) 1395
BsgI GTGCAG 1 cut(s) 611
Bsh1236I CGCG 4 cut(s) 148, 262, 343, 1682
BshFI GGCC 6 cut(s) 314, 369, 432, 1058, 1776, 1846
BshVI ATCGAT 1 cut(s) 1872
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 3 cut(s) 51, 429, 1189
BslFI GGGAC 1 cut(s) 1107
BslI CCNNNNNNNGG 5 cut(s) 376, 391, 461, 1575, 1853
BsmAI GTCTC 3 cut(s) 285, 714, 1466
BsmFI GGGAC 1 cut(s) 1107
BsnI GGCC 6 cut(s) 314, 369, 432, 1058, 1776, 1846
BsoBI CYCGRG 1 cut(s) 401
Bsp119I TTCGAA 1 cut(s) 1465
Bsp1286I GDGCHC 1 cut(s) 1418
Bsp143I GATC 2 cut(s) 545, 1519
BspANI GGCC 6 cut(s) 314, 369, 432, 1058, 1776, 1846
BspCNI CTCAG 5 cut(s) 151, 295, 322, 724, 1315
BspDI ATCGAT 1 cut(s) 1872
BspFNI CGCG 4 cut(s) 148, 262, 343, 1682
BspHI TCATGA 1 cut(s) 1078
BspLI GGNNCC 3 cut(s) 517, 1095, 1423
BspMAI CTGCAG 1 cut(s) 1695
BspMI ACCTGC 1 cut(s) 463
BspQI GCTCTTC 2 cut(s) 38, 1290
BspT104I TTCGAA 1 cut(s) 1465
BsrBI CCGCTC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 472
BsrFI RCCGGY 1 cut(s) 50
BsrI ACTGG 6 cut(s) 556, 1349, 1355, 1608, 1651, 1893
BssAI RCCGGY 1 cut(s) 50
BssECI CCNNGG 3 cut(s) 370, 433, 913
BssMI GATC 2 cut(s) 545, 1519
BssNAI GTATAC 1 cut(s) 1514
BssSI CACGAG 1 cut(s) 1619
BssT1I CCWWGG 2 cut(s) 370, 913
Bst1107I GTATAC 1 cut(s) 1514
Bst2BI CACGAG 1 cut(s) 1619
Bst2UI CCWGG 3 cut(s) 386, 435, 1722
Bst4CI ACNGT 3 cut(s) 171, 1278, 1751
Bst6I CTCTTC 3 cut(s) 38, 993, 1290
BstAPI GCANNNNNTGC 1 cut(s) 455
BstBI TTCGAA 1 cut(s) 1465
BstC8I GCNNGC 8 cut(s) 312, 358, 367, 886, 890, 1414, 1664, 1756
BstDEI CTNAG 8 cut(s) 138, 282, 306, 321, 330, 711, 1301, 1323
BstENI CCTNNNNNAGG 2 cut(s) 374, 459
BstF5I GGATG 6 cut(s) 160, 622, 790, 1256, 1702, 1897
BstFNI CGCG 4 cut(s) 148, 262, 343, 1682
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 5 cut(s) 50, 150, 264, 555, 1266
BstKTI GATC 2 cut(s) 548, 1522
BstMAI GTCTC 3 cut(s) 285, 714, 1466
BstMBI GATC 2 cut(s) 545, 1519
BstNI CCWGG 3 cut(s) 386, 435, 1722
BstNSI RCATGY 1 cut(s) 1666
BstSCI CCNGG 4 cut(s) 384, 433, 1187, 1720
BstSFI CTRYAG 1 cut(s) 1691
BstUI CGCG 4 cut(s) 148, 262, 343, 1682
BstV1I GCAGC 8 cut(s) 100, 420, 461, 604, 814, 1626, 1648, 1677
BstV2I GAAGAC 4 cut(s) 113, 192, 1631, 1683
BstXI CCANNNNNNTGG 2 cut(s) 613, 994
BstZ17I GTATAC 1 cut(s) 1514
Bsu15I ATCGAT 1 cut(s) 1872
BsuI GTATCC 1 cut(s) 1213
BsuRI GGCC 6 cut(s) 314, 369, 432, 1058, 1776, 1846
BsuTUI ATCGAT 1 cut(s) 1872
BtsCI GGATG 6 cut(s) 160, 622, 790, 1256, 1702, 1897
BtsI GCAGTG 2 cut(s) 367, 1092
BtsIMutI CAGTG 7 cut(s) 367, 719, 1092, 1342, 1644, 1756, 1886
BveI ACCTGC 1 cut(s) 463
Cac8I GCNNGC 8 cut(s) 312, 358, 367, 886, 890, 1414, 1664, 1756
CaiI CAGNNNCTG 2 cut(s) 455, 808
CciI TCATGA 1 cut(s) 1078
CfoI GCGC 5 cut(s) 50, 150, 264, 555, 1266
Cfr10I RCCGGY 1 cut(s) 50
Cfr13I GGNCC 8 cut(s) 431, 727, 862, 984, 1094, 1121, 1774, 1845
ClaI ATCGAT 1 cut(s) 1872
CseI GACGC 2 cut(s) 881, 1688
Csp6I GTAC 2 cut(s) 33, 1611
CviQI GTAC 2 cut(s) 33, 1611
DdeI CTNAG 8 cut(s) 138, 282, 306, 321, 330, 711, 1301, 1323
DpnI GATC 2 cut(s) 547, 1521
DpnII GATC 2 cut(s) 545, 1519
DraI TTTAAA 1 cut(s) 966
DraIII CACNNNGTG 1 cut(s) 587
Eam1104I CTCTTC 3 cut(s) 38, 993, 1290
EarI CTCTTC 3 cut(s) 38, 993, 1290
Eco130I CCWWGG 2 cut(s) 370, 913
Eco24I GRGCYC 1 cut(s) 1418
Eco32I GATATC 2 cut(s) 61, 1315
Eco47I GGWCC 5 cut(s) 727, 862, 984, 1094, 1121
Eco57I CTGAAG 4 cut(s) 788, 797, 1476, 1695
Eco88I CYCGRG 1 cut(s) 401
EcoNI CCTNNNNNAGG 2 cut(s) 374, 459
EcoO109I RGGNCCY 2 cut(s) 1094, 1845
EcoRI GAATTC 3 cut(s) 242, 255, 424
EcoRII CCWGG 3 cut(s) 384, 433, 1720
EcoRV GATATC 2 cut(s) 61, 1315
EcoT14I CCWWGG 2 cut(s) 370, 913
EcoT38I GRGCYC 1 cut(s) 1418
ErhI CCWWGG 2 cut(s) 370, 913
FalI AAGNNNNNCTT 6 cut(s) 86, 118, 558, 590, 1284, 1316
FaqI GGGAC 1 cut(s) 1107
FauI CCCGC 1 cut(s) 1405
FblI GTMKAC 1 cut(s) 1513
FokI GGATG 6 cut(s) 167, 629, 797, 1243, 1709, 1904
FriOI GRGCYC 1 cut(s) 1418
FspBI CTAG 3 cut(s) 1043, 1563, 1761
GlaI GCGC 5 cut(s) 49, 149, 263, 554, 1265
GsaI CCCAGC 1 cut(s) 1399
GsuI CTGGAG 1 cut(s) 1668
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 6 cut(s) 314, 369, 432, 1058, 1776, 1846
HapII CCGG 3 cut(s) 51, 429, 1189
HgaI GACGC 2 cut(s) 881, 1688
HhaI GCGC 5 cut(s) 50, 150, 264, 555, 1266
Hin6I GCGC 5 cut(s) 48, 148, 262, 553, 1264
HinP1I GCGC 5 cut(s) 48, 148, 262, 553, 1264
HindIII AAGCTT 3 cut(s) 100, 919, 1013
HinfI GANTC 9 cut(s) 196, 230, 707, 1116, 1427, 1462, 1717, 1735, 1914
HpaII CCGG 3 cut(s) 51, 429, 1189
HphI GGTGA 5 cut(s) 346, 917, 1015, 1529, 1581
Hpy166II GTNNAC 5 cut(s) 828, 1030, 1514, 1602, 1909
Hpy188III TCNNGA 7 cut(s) 403, 896, 930, 1043, 1079, 1621, 1739
Hpy8I GTNNAC 5 cut(s) 828, 1030, 1514, 1602, 1909
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 2 cut(s) 772, 896
HpyCH4III ACNGT 3 cut(s) 171, 1278, 1751
HpyCH4V TGCA 9 cut(s) 476, 529, 592, 702, 802, 884, 1148, 1487, 1693
HpyF3I CTNAG 8 cut(s) 138, 282, 306, 321, 330, 711, 1301, 1323
HspAI GCGC 5 cut(s) 48, 148, 262, 553, 1264
Kzo9I GATC 2 cut(s) 545, 1519
LguI GCTCTTC 2 cut(s) 38, 1290
LmnI GCTCC 5 cut(s) 5, 299, 405, 638, 1271
Lsp1109I GCAGC 8 cut(s) 100, 420, 461, 604, 814, 1626, 1648, 1677
LweI GCATC 3 cut(s) 781, 789, 1677
MaeI CTAG 3 cut(s) 1043, 1563, 1761
MaeIII GTNAC 4 cut(s) 905, 1587, 1793, 1830
MalI GATC 2 cut(s) 547, 1521
MbiI CCGCTC 1 cut(s) 8
MboI GATC 2 cut(s) 545, 1519
MboII GAAGA 9 cut(s) 55, 118, 197, 665, 911, 1010, 1307, 1636, 1688
MhlI GDGCHC 1 cut(s) 1418
MlyI GAGTC 6 cut(s) 239, 716, 1110, 1436, 1744, 1908
MmeI TCCRAC 2 cut(s) 616, 840
MroXI GAANNNNTTC 1 cut(s) 1461
MseI TTAA 7 cut(s) 213, 510, 626, 935, 957, 965, 1290
MspA1I CMGCKG 2 cut(s) 1127, 1639
MspI CCGG 3 cut(s) 51, 429, 1189
MspR9I CCNGG 4 cut(s) 386, 435, 1189, 1722
MvaI CCWGG 3 cut(s) 386, 435, 1722
MvnI CGCG 4 cut(s) 148, 262, 343, 1682
NciI CCSGG 1 cut(s) 1189
NdeII GATC 2 cut(s) 545, 1519
NlaIV GGNNCC 3 cut(s) 517, 1095, 1423
NmuCI GTSAC 2 cut(s) 905, 1587
NspI RCATGY 1 cut(s) 1666
NspV TTCGAA 1 cut(s) 1465
PaeI GCATGC 1 cut(s) 1666
PagI TCATGA 1 cut(s) 1078
PaqCI CACCTGC 1 cut(s) 463
PciSI GCTCTTC 2 cut(s) 38, 1290
PcsI WCGNNNNNNNCGW 1 cut(s) 32
PdmI GAANNNNTTC 1 cut(s) 1461
PfeI GAWTC 3 cut(s) 196, 1462, 1717
PflMI CCANNNNNTGG 1 cut(s) 391
PleI GAGTC 6 cut(s) 238, 715, 1110, 1435, 1743, 1908
PpsI GAGTC 6 cut(s) 238, 715, 1110, 1435, 1743, 1908
PpuMI RGGWCCY 1 cut(s) 1094
PshBI ATTAAT 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 1094
Psp6I CCWGG 3 cut(s) 384, 433, 1720
PspFI CCCAGC 1 cut(s) 1395
PspGI CCWGG 3 cut(s) 384, 433, 1720
PspN4I GGNNCC 3 cut(s) 517, 1095, 1423
PspPI GGNCC 8 cut(s) 431, 727, 862, 984, 1094, 1121, 1774, 1845
PspPPI RGGWCCY 1 cut(s) 1094
PstI CTGCAG 1 cut(s) 1695
PstNI CAGNNNCTG 2 cut(s) 455, 808
PvuII CAGCTG 2 cut(s) 1127, 1639
RsaI GTAC 2 cut(s) 34, 1612
RsaNI GTAC 2 cut(s) 33, 1611
SapI GCTCTTC 2 cut(s) 38, 1290
SaqAI TTAA 7 cut(s) 213, 510, 626, 935, 957, 965, 1290
Sau3AI GATC 2 cut(s) 545, 1519
Sau96I GGNCC 8 cut(s) 431, 727, 862, 984, 1094, 1121, 1774, 1845
SchI GAGTC 6 cut(s) 239, 716, 1110, 1436, 1744, 1908
ScrFI CCNGG 4 cut(s) 386, 435, 1189, 1722
SduI GDGCHC 1 cut(s) 1418
SfaNI GCATC 3 cut(s) 781, 789, 1677
SfcI CTRYAG 1 cut(s) 1691
SfuI TTCGAA 1 cut(s) 1465
SinI GGWCC 5 cut(s) 727, 862, 984, 1094, 1121
SmlI CTYRAG 1 cut(s) 922
SmoI CTYRAG 1 cut(s) 922
SphI GCATGC 1 cut(s) 1666
SspMI CTAG 3 cut(s) 1043, 1563, 1761
StyD4I CCNGG 4 cut(s) 384, 433, 1187, 1720
StyI CCWWGG 2 cut(s) 370, 913
TaaI ACNGT 3 cut(s) 171, 1278, 1751
TaqI TCGA 4 cut(s) 26, 1465, 1518, 1872
TaqII GACCGA 2 cut(s) 744, 879
TatI WGTACW 1 cut(s) 1610
TauI GCSGC 4 cut(s) 120, 317, 320, 1243
TfiI GAWTC 3 cut(s) 196, 1462, 1717
Tru1I TTAA 7 cut(s) 213, 510, 626, 935, 957, 965, 1290
Tru9I TTAA 7 cut(s) 213, 510, 626, 935, 957, 965, 1290
TscAI CASTG 7 cut(s) 367, 719, 1092, 1349, 1651, 1756, 1893
TseFI GTSAC 2 cut(s) 905, 1587
TseI GCWGC 8 cut(s) 88, 408, 449, 592, 802, 1636, 1639, 1690
Tsp45I GTSAC 2 cut(s) 905, 1587
TspDTI ATGAA 7 cut(s) 255, 483, 633, 842, 1095, 1274, 1598
TspGWI ACGGA 4 cut(s) 117, 267, 1729, 1926
TspRI CASTG 7 cut(s) 367, 719, 1092, 1349, 1651, 1756, 1893
Van91I CCANNNNNTGG 1 cut(s) 391
VpaK11BI GGWCC 5 cut(s) 727, 862, 984, 1094, 1121
VspI ATTAAT 1 cut(s) 213
XagI CCTNNNNNAGG 2 cut(s) 374, 459
XapI RAATTY 5 cut(s) 242, 255, 418, 424, 663
XbaI TCTAGA 1 cut(s) 1042
XceI RCATGY 1 cut(s) 1666
XcmI CCANNNNNNNNNTGG 1 cut(s) 1388
XmiI GTMKAC 1 cut(s) 1513
XmnI GAANNNNTTC 1 cut(s) 1461
XspI CTAG 3 cut(s) 1043, 1563, 1761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.