RLG00000023207

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0010330
Physical Location & Seq
Forward (+)
19966660 .. 19972565
5906 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023207

Sequence Viewer

Length: 1944 bp
ATGGGAGGCCTTTGTTCCAAGAGCTCCCGCGCGGCGGTCAACGCCCCCGCTGCCTCCAACCCTTGGGGAGGCGACAATCGGCGCCAAGTCGTCGATGATTACAAACTCAGAAGCTCCAAGAGGGCCACCACCGCCGCCGTCACTGAGGTGGCTCCGCCGCCGGCCATGGATATAAATACTACTCAGCAACATCAGCAGCCTGCTAGATACTCCCACGACGGTCCCGCACTTGTCTATGAAGGAGGAGCTGATACTACTGACGATTTCTACGATGGGATTCCCCGTTATAAATCCAGCTCTTTCAGGTCTAAGGCCAAGGTTTCTGAGGTGGGATCACGCATAGGCAGAGGGGCGGTATCGGTGCTGGACACGCTTGGGAGCAGCATGACGAATTTGAATTCCGGCGCCGGTTTCAATTCAGCACCGCCGGCCAAGGGGAATGAGATTGCAATTATCGCATTCGAGATTGCAAACACTGTTGTTAAGGGTTACAATCTTATGCAATCTGTTTCGCCCAGAAGTATTAAGAATCTGAAAGAAGTGGTGTTTCCTTCGGAAGGTGTGCAGTATTTAGTATCCACAGATACGGAAGAGTTGATGAGGATTGTTGCAGCTGATAAAAGGGAGGAGTTAAAGATTTTTTCGGGGGAGGTGGTTCGTTTTGGAAATCGATGTAAAGATCCTCAGTGGCACAACTTGGACAGATATTTTGAGAAAATCAGCAGAGAACTTATTCCTCAGAAGCATCTGAAGGATGAGGCCAGTTCGGTAATGCAGCAACTGATGACATTGGTCCAGTGTACTGCTGAATTATACCATGAGGCCAATGCATTGGACCGATTTGAACAAGACTATGAACGTAAGCGGTTCGAGGAGAGTATTTCATCTTCCACTGACAGAGGCGATACCCTTGGAATCTTGAGGTCTGAACTAAAAAGCCAAAGGAAACTAGTTAAAAGTTTAAAGAAAAAGTCACTTTGGTCCAAAAGTATGGAAGAGGTGATGGAAAAGCTTGTGGAAATAGTTCACTTTTTATATCTAGAGATACATGCTGCCTTTGGTAGTGCTGAGGGTCAGAAAACCTTGGAGGGAGCTACGAATAAACATCAAAAGTTGGGACCTGCTGGACTTTCTTTACATTATGCAAATATCGTTCTTCAAATCGATACTCTTGTAGTCCGGTCAAGTGCTCCGCCTCCAACTACAAGAGATACATTATACCAGAGCCTGCCACCTAATATAAAATCATCTTTACGCTCCAAATTACACTCTTTCCATATTAAAGAAGAGCTCACTATCACAGAAATCAAAGCTGAGATGGAGAAAACTCTGCACTGGCTTGTTCCAATGGCTGCAAACACAGCCAAAGATCACCATGGTTTTGGCTGGGTAGGTGAGTGGGCGAGTACTGGTTCTGAGACGAACCGTAAACCAGCTCCACAGACTGAGGTGATTCGGATTGAGACATTCCATCATGCAAATAGGGAAAAAACAGAAGCCTGTGTACTCAAACTAGTATTGTGGCTTCATTATTTGGTCTCTCAAAGTAAGCCTGCATCCGCTAGTGTGGGTATGCGGTCACCTGTTAAGTCCATAGCTACCAAAACCCTCCCAAAGACAAATGAGCAGCTCAAGGACATTCCCCCCTCAACCATGCTAACAACTGACGACCAGGAGATGCTGCAGGATGTCAGTAAGAGAAGACGGGTTCCCGGAATTAGTAAAAGTCAGGACTTTGACAACGCAAACTCTAGTTTGGGAAACCGGAGCAGATTGAACAAGAGTAACAGCTATACATCTGGGGAAGTGACCAAGAAATTGTTACCTTTCAGTAGGCTCTCTTCTGGGGTTCCTGTCATTGATTTTGGTATAGATAAGGAAAAAGCCTTGGATGTCATAGATGGAGTGGACACACAGAGAGATCATCATCATCCAAGGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

648

Amino Acids

71.94

Weight (kDa)

8.94

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 151 - 207 1.4e-20 Domain of unknown function (DUF3475)
DUF668 PF05003 372 - 456 1.3e-30 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08660 AT5G08660 AT5G08660 AT5G08660
fragaria_vesca FvH4_1g25750 FvH4_1g25750
malus_domestica MD15G1140000.v1.1
prunus_persica Prupe.4G285700_v2.0.a1 Prupe.4G285700_v2.0.a1 Prupe.4G285700_v2.0.a1
pyrus_communis pycom15g12650
rosa_chinensis RchiOBHm_Chr3g0484691
rosa_laevigata RLG00000023207
rosa_multiflora Rmu_sc0006192.1_g000003
rosa_roxburghii Rroxscaffold_6G00397520
rosa_rugosa Rorug03G0213500
rosa_samantha Rh3AG263600 Rh3BG298800 Rh3CG296200 Rh3DG292300
rosa_wichuraiana Rw3G023510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 288
Acc36I ACCTGC 1 cut(s) 1129
AccB1I GGYRCC 2 cut(s) 81, 404
AccB7I CCANNNNNTGG 1 cut(s) 63
AccII CGCG 2 cut(s) 30, 32
AclWI GGATC 2 cut(s) 340, 674
AcoI YGGCCR 2 cut(s) 162, 429
AcsI RAATTY 2 cut(s) 391, 397
AcuI CTGAAG 1 cut(s) 770
AcyI GRCGYC 2 cut(s) 82, 405
AfaI GTAC 3 cut(s) 802, 1408, 1506
AfiI CCNNNNNNNGG 5 cut(s) 34, 63, 68, 434, 557
AgsI TTSAA 5 cut(s) 397, 415, 845, 1160, 1777
AhlI ACTAGT 2 cut(s) 949, 1513
AjnI CCWGG 1 cut(s) 1671
AleI CACNNNNGTG 1 cut(s) 146
Alw21I GWGCWC 3 cut(s) 26, 1192, 1293
Alw26I GTCTC 3 cut(s) 1412, 1457, 1543
AlwI GGATC 2 cut(s) 340, 674
AlwNI CAGNNNCTG 2 cut(s) 781, 1228
AoxI GGCC 7 cut(s) 7, 123, 162, 312, 429, 759, 822
ApeKI GCWGC 9 cut(s) 50, 196, 381, 611, 775, 1052, 1352, 1627, 1681
ApoI RAATTY 2 cut(s) 391, 397
ArsI GACNNNNNNTTYG 2 cut(s) 692, 724
Asp700I GAANNNNTTC 2 cut(s) 732, 1023
AspLEI GCGC 3 cut(s) 32, 84, 407
AspS9I GGNCC 6 cut(s) 123, 221, 793, 835, 981, 1118
AsuC2I CCSGG 1 cut(s) 1713
AsuHPI GGTGA 5 cut(s) 1012, 1364, 1406, 1462, 1572
AvaII GGWCC 5 cut(s) 221, 793, 835, 981, 1118
BaeI ACNNNNGTAYC 2 cut(s) 1158, 1191
BanI GGYRCC 2 cut(s) 81, 404
BanII GRGCYC 2 cut(s) 26, 1293
BarI GAAGNNNNNNTAC 8 cut(s) 871, 903, 1488, 1509, 1520, 1541, 1825, 1857
BbsI GAAGAC 1 cut(s) 1708
Bbv12I GWGCWC 3 cut(s) 26, 1192, 1293
BbvCI CCTCAGC 1 cut(s) 1068
BbvI GCAGC 9 cut(s) 37, 208, 393, 623, 787, 1039, 1339, 1639, 1668
BccI CCATC 5 cut(s) 266, 997, 1312, 1479, 1895
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 1 cut(s) 1673
BciVI GTATCC 1 cut(s) 586
BcnI CCSGG 1 cut(s) 1713
BcoDI GTCTC 3 cut(s) 1412, 1457, 1543
BcuI ACTAGT 2 cut(s) 949, 1513
BfaI CTAG 6 cut(s) 204, 950, 1040, 1514, 1563, 1752
BfmI CTRYAG 1 cut(s) 1682
BfoI RGCGCY 2 cut(s) 85, 408
BfuAI ACCTGC 1 cut(s) 1129
BfuI GTATCC 1 cut(s) 586
BmcAI AGTACT 1 cut(s) 1408
Bme1390I CCNGG 2 cut(s) 1673, 1713
Bme18I GGWCC 5 cut(s) 221, 793, 835, 981, 1118
BmgT120I GGNCC 6 cut(s) 123, 221, 793, 835, 981, 1118
BmiI GGNNCC 7 cut(s) 83, 153, 223, 406, 1119, 1710, 1851
BmrFI CCNGG 2 cut(s) 1673, 1713
BmsI GCATC 3 cut(s) 754, 1565, 1668
BoxI GACNNNNGTC 1 cut(s) 791
BpiI GAAGAC 1 cut(s) 1708
Bpu10I CCTNAGC 1 cut(s) 1068
BpuEI CTTGAG 2 cut(s) 940, 1616
BpuMI CCSGG 1 cut(s) 1713
Bsa29I ATCGAT 2 cut(s) 670, 1164
BsaBI GATNNNNATC 1 cut(s) 1926
BsaHI GRCGYC 2 cut(s) 82, 405
BsaI GGTCTC 1 cut(s) 1543
BsaJI CCNNGG 9 cut(s) 62, 165, 315, 432, 910, 1083, 1375, 1887, 1934
BsaWI WCCGGW 2 cut(s) 1179, 1764
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 63, 68, 434, 557
Bse118I RCCGGY 3 cut(s) 160, 407, 427
Bse1I ACTGG 4 cut(s) 762, 796, 1340, 1414
Bse8I GATNNNNATC 1 cut(s) 1926
BseBI CCWGG 1 cut(s) 1673
BseCI ATCGAT 2 cut(s) 670, 1164
BseDI CCNNGG 9 cut(s) 62, 165, 315, 432, 910, 1083, 1375, 1887, 1934
BseGI GGATG 5 cut(s) 760, 1556, 1693, 1897, 1930
BseJI GATNNNNATC 1 cut(s) 1926
BseLI CCNNNNNNNGG 5 cut(s) 34, 63, 68, 434, 557
BseNI ACTGG 4 cut(s) 762, 796, 1340, 1414
BseRI GAGGAG 3 cut(s) 258, 641, 887
BseXI GCAGC 9 cut(s) 37, 208, 393, 623, 787, 1039, 1339, 1639, 1668
BseYI CCCAGC 1 cut(s) 1386
BsgI GTGCAG 2 cut(s) 584, 1316
Bsh1236I CGCG 2 cut(s) 30, 32
BshFI GGCC 7 cut(s) 9, 125, 164, 314, 431, 761, 824
BshNI GGYRCC 2 cut(s) 81, 404
BshVI ATCGAT 2 cut(s) 670, 1164
BsiHKAI GWGCWC 3 cut(s) 26, 1192, 1293
BsiSI CCGG 7 cut(s) 161, 402, 408, 428, 1180, 1713, 1765
BslFI GGGAC 2 cut(s) 207, 1131
BslI CCNNNNNNNGG 5 cut(s) 34, 63, 68, 434, 557
BsmAI GTCTC 3 cut(s) 1412, 1457, 1543
BsmBI CGTCTC 1 cut(s) 1412
BsmFI GGGAC 2 cut(s) 207, 1131
BsmI GAATGC 1 cut(s) 458
BsnI GGCC 7 cut(s) 9, 125, 164, 314, 431, 761, 824
Bso31I GGTCTC 1 cut(s) 1543
Bsp1286I GDGCHC 3 cut(s) 26, 1192, 1293
Bsp143I GATC 4 cut(s) 332, 679, 1369, 1921
Bsp19I CCATGG 2 cut(s) 165, 1375
BspANI GGCC 7 cut(s) 9, 125, 164, 314, 431, 761, 824
BspDI ATCGAT 2 cut(s) 670, 1164
BspFNI CGCG 2 cut(s) 30, 32
BspLI GGNNCC 7 cut(s) 83, 153, 223, 406, 1119, 1710, 1851
BspMAI CTGCAG 1 cut(s) 1686
BspMI ACCTGC 1 cut(s) 1129
BspPI GGATC 2 cut(s) 340, 674
BspQI GCTCTTC 1 cut(s) 1281
BspT107I GGYRCC 2 cut(s) 81, 404
BspTNI GGTCTC 1 cut(s) 1543
BsrFI RCCGGY 3 cut(s) 160, 407, 427
BsrI ACTGG 4 cut(s) 762, 796, 1340, 1414
BssAI RCCGGY 3 cut(s) 160, 407, 427
BssECI CCNNGG 9 cut(s) 62, 165, 315, 432, 910, 1083, 1375, 1887, 1934
BssMI GATC 4 cut(s) 332, 679, 1369, 1921
BssNI GRCGYC 2 cut(s) 82, 405
BssT1I CCWWGG 9 cut(s) 62, 165, 315, 432, 910, 1083, 1375, 1887, 1934
Bst2UI CCWGG 1 cut(s) 1673
Bst4CI ACNGT 3 cut(s) 221, 478, 1427
Bst6I CTCTTC 4 cut(s) 585, 990, 1281, 1846
BstACI GRCGYC 2 cut(s) 82, 405
BstC8I GCNNGC 5 cut(s) 162, 201, 429, 1229, 1554
BstDSI CCRYGG 2 cut(s) 165, 1375
BstEII GGTNACC 1 cut(s) 1578
BstENI CCTNNNNNAGG 2 cut(s) 66, 555
BstF5I GGATG 5 cut(s) 760, 1556, 1693, 1897, 1930
BstFNI CGCG 2 cut(s) 30, 32
BstH2I RGCGCY 2 cut(s) 85, 408
BstHHI GCGC 3 cut(s) 32, 84, 407
BstKTI GATC 4 cut(s) 335, 682, 1372, 1924
BstMAI GTCTC 3 cut(s) 1412, 1457, 1543
BstMBI GATC 4 cut(s) 332, 679, 1369, 1921
BstMWI GCNNNNNNNGC 8 cut(s) 41, 50, 131, 193, 370, 428, 455, 1361
BstNI CCWGG 1 cut(s) 1673
BstNSI RCATGY 1 cut(s) 1052
BstPAI GACNNNNGTC 1 cut(s) 791
BstPI GGTNACC 1 cut(s) 1578
BstSCI CCNGG 2 cut(s) 1671, 1711
BstSFI CTRYAG 1 cut(s) 1682
BstUI CGCG 2 cut(s) 30, 32
BstV1I GCAGC 9 cut(s) 37, 208, 393, 623, 787, 1039, 1339, 1639, 1668
BstV2I GAAGAC 1 cut(s) 1708
BstX2I RGATCY 1 cut(s) 679
BstXI CCANNNNNNTGG 3 cut(s) 832, 991, 1382
BstYI RGATCY 1 cut(s) 679
Bsu15I ATCGAT 2 cut(s) 670, 1164
BsuI GTATCC 1 cut(s) 586
BsuRI GGCC 7 cut(s) 9, 125, 164, 314, 431, 761, 824
BsuTUI ATCGAT 2 cut(s) 670, 1164
BtgI CCRYGG 2 cut(s) 165, 1375
BtsCI GGATG 5 cut(s) 760, 1556, 1693, 1897, 1930
BtsIMutI CAGTG 6 cut(s) 141, 474, 692, 803, 891, 1333
BveI ACCTGC 1 cut(s) 1129
Cac8I GCNNGC 5 cut(s) 162, 201, 429, 1229, 1554
CaiI CAGNNNCTG 2 cut(s) 781, 1228
CfoI GCGC 3 cut(s) 32, 84, 407
Cfr10I RCCGGY 3 cut(s) 160, 407, 427
Cfr13I GGNCC 6 cut(s) 123, 221, 793, 835, 981, 1118
ClaI ATCGAT 2 cut(s) 670, 1164
Csp6I GTAC 3 cut(s) 801, 1407, 1505
CviAII CATG 7 cut(s) 166, 385, 818, 1049, 1376, 1475, 1654
CviQI GTAC 3 cut(s) 801, 1407, 1505
DinI GGCGCC 2 cut(s) 83, 406
DpnI GATC 4 cut(s) 334, 681, 1371, 1923
DpnII GATC 4 cut(s) 332, 679, 1369, 1921
DraI TTTAAA 1 cut(s) 963
EaeI YGGCCR 2 cut(s) 162, 429
Eam1104I CTCTTC 4 cut(s) 585, 990, 1281, 1846
EarI CTCTTC 4 cut(s) 585, 990, 1281, 1846
EciI GGCGGA 2 cut(s) 144, 1182
Ecl136II GAGCTC 2 cut(s) 24, 1291
Eco130I CCWWGG 9 cut(s) 62, 165, 315, 432, 910, 1083, 1375, 1887, 1934
Eco147I AGGCCT 1 cut(s) 9
Eco24I GRGCYC 2 cut(s) 26, 1293
Eco31I GGTCTC 1 cut(s) 1543
Eco47I GGWCC 5 cut(s) 221, 793, 835, 981, 1118
Eco53kI GAGCTC 2 cut(s) 24, 1291
Eco57I CTGAAG 1 cut(s) 770
Eco91I GGTNACC 1 cut(s) 1578
EcoICRI GAGCTC 2 cut(s) 24, 1291
EcoNI CCTNNNNNAGG 2 cut(s) 66, 555
EcoO109I RGGNCCY 1 cut(s) 1118
EcoO65I GGTNACC 1 cut(s) 1578
EcoRI GAATTC 1 cut(s) 397
EcoRII CCWGG 1 cut(s) 1671
EcoT14I CCWWGG 9 cut(s) 62, 165, 315, 432, 910, 1083, 1375, 1887, 1934
EcoT22I ATGCAT 1 cut(s) 832
EcoT38I GRGCYC 2 cut(s) 26, 1293
EgeI GGCGCC 2 cut(s) 83, 406
EheI GGCGCC 2 cut(s) 83, 406
ErhI CCWWGG 9 cut(s) 62, 165, 315, 432, 910, 1083, 1375, 1887, 1934
Esp3I CGTCTC 1 cut(s) 1412
FaeI CATG 7 cut(s) 169, 388, 821, 1052, 1379, 1478, 1657
FaqI GGGAC 2 cut(s) 207, 1131
FatI CATG 7 cut(s) 165, 384, 817, 1048, 1375, 1474, 1653
FauI CCCGC 3 cut(s) 35, 55, 232
FokI GGATG 5 cut(s) 767, 1543, 1700, 1904, 1917
FriOI GRGCYC 2 cut(s) 26, 1293
FspBI CTAG 6 cut(s) 204, 950, 1040, 1514, 1563, 1752
GlaI GCGC 3 cut(s) 31, 83, 406
GsaI CCCAGC 1 cut(s) 1390
HaeII RGCGCY 2 cut(s) 85, 408
HaeIII GGCC 7 cut(s) 9, 125, 164, 314, 431, 761, 824
HapII CCGG 7 cut(s) 161, 402, 408, 428, 1180, 1713, 1765
HhaI GCGC 3 cut(s) 32, 84, 407
Hin1I GRCGYC 2 cut(s) 82, 405
Hin1II CATG 7 cut(s) 169, 388, 821, 1052, 1379, 1478, 1657
Hin6I GCGC 3 cut(s) 30, 82, 405
HinP1I GCGC 3 cut(s) 30, 82, 405
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 1010
HinfI GANTC 4 cut(s) 277, 529, 915, 1453
HpaII CCGG 7 cut(s) 161, 402, 408, 428, 1180, 1713, 1765
HphI GGTGA 5 cut(s) 1012, 1364, 1406, 1462, 1572
Hpy166II GTNNAC 6 cut(s) 40, 801, 1027, 1430, 1505, 1909
Hpy188III TCNNGA 4 cut(s) 463, 919, 1040, 1730
Hpy8I GTNNAC 6 cut(s) 40, 801, 1027, 1430, 1505, 1909
Hpy99I CGWCG 2 cut(s) 95, 221
HpyAV CCTTC 4 cut(s) 233, 551, 561, 745
HpyCH4III ACNGT 3 cut(s) 221, 478, 1427
HpyCH4IV ACGT 1 cut(s) 859
HpyF10VI GCNNNNNNNGC 8 cut(s) 41, 50, 131, 193, 370, 428, 455, 1361
HpySE526I ACGT 1 cut(s) 859
Hsp92I GRCGYC 2 cut(s) 82, 405
Hsp92II CATG 7 cut(s) 169, 388, 821, 1052, 1379, 1478, 1657
HspAI GCGC 3 cut(s) 30, 82, 405
KasI GGCGCC 2 cut(s) 81, 404
KroI GCCGGC 2 cut(s) 160, 427
KroNI GCCGGC 2 cut(s) 162, 429
Kzo9I GATC 4 cut(s) 332, 679, 1369, 1921
LguI GCTCTTC 1 cut(s) 1281
Lsp1109I GCAGC 9 cut(s) 37, 208, 393, 623, 787, 1039, 1339, 1639, 1668
LweI GCATC 3 cut(s) 754, 1565, 1668
MaeI CTAG 6 cut(s) 204, 950, 1040, 1514, 1563, 1752
MaeII ACGT 1 cut(s) 859
MaeIII GTNAC 7 cut(s) 139, 488, 972, 1578, 1784, 1807, 1821
MalI GATC 4 cut(s) 334, 681, 1371, 1923
MboI GATC 4 cut(s) 332, 679, 1369, 1921
MboII GAAGA 7 cut(s) 602, 879, 1007, 1148, 1298, 1713, 1833
MflI RGATCY 1 cut(s) 679
MhlI GDGCHC 3 cut(s) 26, 1192, 1293
MluCI AATT 8 cut(s) 391, 397, 415, 450, 809, 1262, 1716, 1817
Mly113I GGCGCC 2 cut(s) 82, 405
MmeI TCCRAC 2 cut(s) 81, 1223
Mph1103I ATGCAT 1 cut(s) 832
MroNI GCCGGC 2 cut(s) 160, 427
MroXI GAANNNNTTC 2 cut(s) 732, 1023
MseI TTAA 7 cut(s) 483, 525, 632, 954, 962, 1281, 1587
MslI CAYNNNNRTG 1 cut(s) 146
MspA1I CMGCKG 2 cut(s) 50, 614
MspI CCGG 7 cut(s) 161, 402, 408, 428, 1180, 1713, 1765
MspR9I CCNGG 2 cut(s) 1673, 1713
Mva1269I GAATGC 1 cut(s) 458
MvaI CCWGG 1 cut(s) 1673
MvnI CGCG 2 cut(s) 30, 32
MwoI GCNNNNNNNGC 8 cut(s) 41, 50, 131, 193, 370, 428, 455, 1361
NaeI GCCGGC 2 cut(s) 162, 429
NarI GGCGCC 2 cut(s) 82, 405
NciI CCSGG 1 cut(s) 1713
NcoI CCATGG 2 cut(s) 165, 1375
NdeII GATC 4 cut(s) 332, 679, 1369, 1921
NgoMIV GCCGGC 2 cut(s) 160, 427
NlaIII CATG 7 cut(s) 169, 388, 821, 1052, 1379, 1478, 1657
NlaIV GGNNCC 7 cut(s) 83, 153, 223, 406, 1119, 1710, 1851
NmuCI GTSAC 4 cut(s) 139, 972, 1578, 1807
NsiI ATGCAT 1 cut(s) 832
NspI RCATGY 1 cut(s) 1052
OliI CACNNNNGTG 1 cut(s) 146
PceI AGGCCT 1 cut(s) 9
PciSI GCTCTTC 1 cut(s) 1281
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 1 cut(s) 458
PdiI GCCGGC 2 cut(s) 162, 429
PdmI GAANNNNTTC 2 cut(s) 732, 1023
PfeI GAWTC 4 cut(s) 277, 529, 915, 1453
PflMI CCANNNNNTGG 1 cut(s) 63
PfoI TCCNGGA 1 cut(s) 1711
PluTI GGCGCC 2 cut(s) 85, 408
PpuMI RGGWCCY 1 cut(s) 1118
PshAI GACNNNNGTC 1 cut(s) 791
PsiI TTATAA 1 cut(s) 288
Psp124BI GAGCTC 2 cut(s) 26, 1293
Psp5II RGGWCCY 1 cut(s) 1118
Psp6I CCWGG 1 cut(s) 1671
PspEI GGTNACC 1 cut(s) 1578
PspFI CCCAGC 1 cut(s) 1386
PspGI CCWGG 1 cut(s) 1671
PspN4I GGNNCC 7 cut(s) 83, 153, 223, 406, 1119, 1710, 1851
PspPI GGNCC 6 cut(s) 123, 221, 793, 835, 981, 1118
PspPPI RGGWCCY 1 cut(s) 1118
PstI CTGCAG 1 cut(s) 1686
PstNI CAGNNNCTG 2 cut(s) 781, 1228
PsuI RGATCY 1 cut(s) 679
PvuII CAGCTG 1 cut(s) 614
RsaI GTAC 3 cut(s) 802, 1408, 1506
RsaNI GTAC 3 cut(s) 801, 1407, 1505
RseI CAYNNNNRTG 1 cut(s) 146
SacI GAGCTC 2 cut(s) 26, 1293
SapI GCTCTTC 1 cut(s) 1281
SaqAI TTAA 7 cut(s) 483, 525, 632, 954, 962, 1281, 1587
Sau3AI GATC 4 cut(s) 332, 679, 1369, 1921
Sau96I GGNCC 6 cut(s) 123, 221, 793, 835, 981, 1118
ScaI AGTACT 1 cut(s) 1408
ScrFI CCNGG 2 cut(s) 1673, 1713
SduI GDGCHC 3 cut(s) 26, 1192, 1293
SfaNI GCATC 3 cut(s) 754, 1565, 1668
SfcI CTRYAG 1 cut(s) 1682
SfoI GGCGCC 2 cut(s) 83, 406
SinI GGWCC 5 cut(s) 221, 793, 835, 981, 1118
SmiMI CAYNNNNRTG 1 cut(s) 146
SmlI CTYRAG 2 cut(s) 919, 1631
SmoI CTYRAG 2 cut(s) 919, 1631
SpeI ACTAGT 2 cut(s) 949, 1513
Sse9I AATT 8 cut(s) 391, 397, 415, 450, 809, 1262, 1716, 1817
SseBI AGGCCT 1 cut(s) 9
SspDI GGCGCC 2 cut(s) 81, 404
SspMI CTAG 6 cut(s) 204, 950, 1040, 1514, 1563, 1752
SstI GAGCTC 2 cut(s) 26, 1293
StuI AGGCCT 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 1671, 1711
StyI CCWWGG 9 cut(s) 62, 165, 315, 432, 910, 1083, 1375, 1887, 1934
TaaI ACNGT 3 cut(s) 221, 478, 1427
TaiI ACGT 1 cut(s) 862
TaqI TCGA 5 cut(s) 93, 462, 670, 870, 1164
TaqII GACCGA 1 cut(s) 852
TasI AATT 8 cut(s) 391, 397, 415, 450, 809, 1262, 1716, 1817
TatI WGTACW 3 cut(s) 800, 1406, 1504
TauI GCSGC 3 cut(s) 35, 137, 160
TfiI GAWTC 4 cut(s) 277, 529, 915, 1453
Tru1I TTAA 7 cut(s) 483, 525, 632, 954, 962, 1281, 1587
Tru9I TTAA 7 cut(s) 483, 525, 632, 954, 962, 1281, 1587
TscAI CASTG 6 cut(s) 148, 481, 692, 803, 898, 1340
TseFI GTSAC 4 cut(s) 139, 972, 1578, 1807
TseI GCWGC 9 cut(s) 50, 196, 381, 611, 775, 1052, 1352, 1627, 1681
Tsp45I GTSAC 4 cut(s) 139, 972, 1578, 1807
TspDTI ATGAA 4 cut(s) 252, 870, 873, 1517
TspGWI ACGGA 1 cut(s) 602
TspRI CASTG 6 cut(s) 148, 481, 692, 803, 898, 1340
Van91I CCANNNNNTGG 1 cut(s) 63
VpaK11BI GGWCC 5 cut(s) 221, 793, 835, 981, 1118
XagI CCTNNNNNAGG 2 cut(s) 66, 555
XapI RAATTY 2 cut(s) 391, 397
XbaI TCTAGA 1 cut(s) 1039
XceI RCATGY 1 cut(s) 1052
XmnI GAANNNNTTC 2 cut(s) 732, 1023
XspI CTAG 6 cut(s) 204, 950, 1040, 1514, 1563, 1752
ZrmI AGTACT 1 cut(s) 1408
Zsp2I ATGCAT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.