Rw3G023510

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0010330
Physical Location & Seq
Reverse (-)
29461547 .. 29467385
5839 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G023510.1

Sequence Viewer

Length: 1947 bp
ATGGGAGGCCTTTGTTCCAAGAGCTCCCGCGCGGCGGTCAACGCCCCCTCCGCCGCCAACCCTTGGGCCGGCGACAATCGGCGCCAAGTCGTCGATGATTACAAACTGAGAAACTCCAAGAGGACCGCCACCGCCGCCGCCACTGAGGTGGCTCCGCCGCCGGCCGTGGATATAAATACTACTCAGCAACAACAGCAGCAGCAGCCTGCTAGATACTCCCACGACGGTCCTGCCCTTGTCTATGAAGGTGGAGCTGATCCTACTGACGATTTCTACGATGGGATCCCCCGTTATAAATCCGGCTCTTTCAGGTCTAAGGCCAAGGTTTCTGAGGTGGGATCACGTATAGGCAGAGGGGCGGTATCGGTGCTGGACACGCTTGGGAGCAGCATGACGAATTTGAATTCCGGCGCCGGTTTCAACTCAGCACCGCCGGCCAAGGGGAATGAGATTGCAATTATCGCATTCGAGATTGCAAACACTGTTGTTAAGGGTTACAATCTTATGCAATCTGTTTCGACCAGAAGTATTAGGAATCTGAAAGAAGTGGTGTTTCCTTCGGAAGGTGTGCAGTATTTGGTATCCACAGATACGGACGAGTTGATGAGGATTGTTGCAGCTGATAAAAGGGAGGAGTTAAAGATTTTCTCGGGGGAGGTGGTTCGTTTTGGAAATCGATGTAAAGATCCTCAGTGGCACAACTTGGACAGATATTTTGAGAAAATAAGCAGAGAACTTATTCCTCAGAAGCATCTGAAGGATGAGGCCAGTTCGGTAATGCAGCAACTGATGACACTAGTCCAATATACTGCTGAATTATACCATGAGTTCCATGCATTGGACCGATTTGAACAAGACTATGAACGTAAGCGGTTCGAGGAGAGTATTTCATCCACTGACAGAGGCGATACCCTTGGAATCATGAGGTCTGAACTAAAAAGCCAAAGGAAACTAGTTAAAAGTTTAAAGAAAAAGTCACTTTGGTCCAAAAGTATGGAAGAGGTGATGGAAAAGCTTGTTGAAATAGTTCACTATTTATATCTAGAGATACATGCTGCCTTTGGTAGTGCTGAGGGTCAGAAAACCGTGGAGGGAGCTACGAATAAACATCAAAAGTTGGGACCTACTGGACTTTCTTTACATTATGCAAATATCGTTCTTCAAATCGATACTCTTGTAGTCCGGTCAAGTGCTCCGCCTCCAACTACAAGAGATACATTATACCAGAGCTTGCCGCCTAATATAAAATCATCTTTACGCTCCAAATTACACTCTTTCCATATTAAAGAAGAGCTCACTATCACAGAAATCAAAGCTGAGATGGAGAAAACTCTGCACTGGCTTGTTCCAATGGCTGCAAACACAGCCAAAGATCACCATGGTTTTGGCTGGGTAGGTGAGTGGGCAAGTACTGGTTCTGAGACGAACCGTAAACCAGCTCCACAGACTGAGGTGATTCGGATTGAGACATTCCATCATGCAAATAGGGAAAAAACAGAAGCCTGTGTACTCAAACTAGTATTGTGGCTTCATTATTTGGTCTCTCAAAGTAAGCCTGCATCCACAAGTGTGGGTATGCGGTCACCTGTTAAGTCCACAGCTACCAAAACCCTCCCAAAGACAAATGAGCAGATCAAGGACATTCCCCCCTCAACCATGCTAACAACTGACGACCAGGAGATGCTGCAGGATGTCAGTAAGAGAAGACGGGTTCCCGGAATTAGTAAAAGTCAGGACTTTGACAACGCAAACTCTAGTTTGGGAAACCGGAGCAGATTGAACAAGAGTAACAGCTATACATCTGGGGAAGAAACCAAGAAATTGTTACCTTTCAGTAGGCTCTCTTCTGGGGTTCCTGTCATTGATTTTGGTATAGATAAGGAAAAAGCCTTGGATGTCATAGATAGAGTGGACACACAGAGAGATCATCATCATCCAAGGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

648

Amino Acids

72.42

Weight (kDa)

8.97

Isoelectric Point (pI)

45.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 153 - 209 3.1e-21 Domain of unknown function (DUF3475)
DUF668 PF05003 373 - 457 3.1e-30 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08660 AT5G08660 AT5G08660 AT5G08660
fragaria_vesca FvH4_1g25750 FvH4_1g25750
malus_domestica MD15G1140000.v1.1
prunus_persica Prupe.4G285700_v2.0.a1 Prupe.4G285700_v2.0.a1 Prupe.4G285700_v2.0.a1
pyrus_communis pycom15g12650
rosa_chinensis RchiOBHm_Chr3g0484691
rosa_laevigata RLG00000023207
rosa_multiflora Rmu_sc0006192.1_g000003
rosa_roxburghii Rroxscaffold_6G00397520
rosa_rugosa Rorug03G0213500
rosa_samantha Rh3AG263600 Rh3BG298800 Rh3CG296200 Rh3DG292300
rosa_wichuraiana Rw3G023510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 294
AccB1I GGYRCC 2 cut(s) 81, 410
AccB7I CCANNNNNTGG 2 cut(s) 63, 838
AccII CGCG 2 cut(s) 30, 32
AclWI GGATC 5 cut(s) 251, 277, 290, 346, 680
AcoI YGGCCR 2 cut(s) 162, 435
AcsI RAATTY 2 cut(s) 397, 403
AcuI CTGAAG 1 cut(s) 776
AcyI GRCGYC 2 cut(s) 82, 411
AfaI GTAC 2 cut(s) 1411, 1509
AfiI CCNNNNNNNGG 6 cut(s) 34, 63, 68, 440, 563, 838
AgsI TTSAA 6 cut(s) 403, 421, 851, 1022, 1163, 1780
AhlI ACTAGT 3 cut(s) 796, 952, 1516
AjnI CCWGG 1 cut(s) 1674
AleI CACNNNNGTG 2 cut(s) 146, 1568
Alw21I GWGCWC 3 cut(s) 26, 1195, 1296
Alw26I GTCTC 3 cut(s) 1415, 1460, 1546
AlwI GGATC 5 cut(s) 251, 277, 290, 346, 680
AlwNI CAGNNNCTG 1 cut(s) 787
Ama87I CYCGRG 1 cut(s) 649
AoxI GGCC 6 cut(s) 7, 66, 162, 318, 435, 765
ApeKI GCWGC 9 cut(s) 196, 199, 202, 387, 617, 781, 1055, 1355, 1684
ApoI RAATTY 2 cut(s) 397, 403
ArsI GACNNNNNNTTYG 2 cut(s) 698, 730
Asp700I GAANNNNTTC 2 cut(s) 738, 1026
AspLEI GCGC 3 cut(s) 32, 84, 413
AspS9I GGNCC 6 cut(s) 66, 123, 227, 841, 984, 1121
AsuC2I CCSGG 1 cut(s) 1716
AsuHPI GGTGA 5 cut(s) 1015, 1367, 1409, 1465, 1575
AvaI CYCGRG 1 cut(s) 649
AvaII GGWCC 5 cut(s) 123, 227, 841, 984, 1121
BaeI ACNNNNGTAYC 2 cut(s) 1161, 1194
BamHI GGATCC 1 cut(s) 282
BanI GGYRCC 2 cut(s) 81, 410
BanII GRGCYC 2 cut(s) 26, 1296
BarI GAAGNNNNNNTAC 6 cut(s) 1491, 1512, 1523, 1544, 1828, 1860
BbsI GAAGAC 1 cut(s) 1711
Bbv12I GWGCWC 3 cut(s) 26, 1195, 1296
BbvCI CCTCAGC 1 cut(s) 1071
BbvI GCAGC 9 cut(s) 208, 211, 214, 399, 629, 793, 1042, 1342, 1671
BccI CCATC 4 cut(s) 272, 1000, 1315, 1482
BceAI ACGGC 1 cut(s) 149
BcgI CGANNNNNNTGC 2 cut(s) 212, 246
BciT130I CCWGG 1 cut(s) 1676
BciVI GTATCC 1 cut(s) 592
BcnI CCSGG 1 cut(s) 1716
BcoDI GTCTC 3 cut(s) 1415, 1460, 1546
BcuI ACTAGT 3 cut(s) 796, 952, 1516
BfaI CTAG 6 cut(s) 210, 797, 953, 1043, 1517, 1755
BfmI CTRYAG 1 cut(s) 1685
BfoI RGCGCY 2 cut(s) 85, 414
BfuI GTATCC 1 cut(s) 592
BmcAI AGTACT 1 cut(s) 1411
Bme1390I CCNGG 2 cut(s) 1676, 1716
Bme18I GGWCC 5 cut(s) 123, 227, 841, 984, 1121
BmeT110I CYCGRG 1 cut(s) 649
BmgT120I GGNCC 6 cut(s) 66, 123, 227, 841, 984, 1121
BmiI GGNNCC 7 cut(s) 83, 153, 284, 412, 1122, 1713, 1854
BmrFI CCNGG 2 cut(s) 1676, 1716
BmsI GCATC 3 cut(s) 760, 1568, 1671
BoxI GACNNNNGTC 1 cut(s) 797
BpiI GAAGAC 1 cut(s) 1711
Bpu10I CCTNAGC 1 cut(s) 1071
BpuMI CCSGG 1 cut(s) 1716
Bsa29I ATCGAT 2 cut(s) 676, 1167
BsaAI YACGTR 1 cut(s) 344
BsaBI GATNNNNATC 1 cut(s) 1929
BsaHI GRCGYC 2 cut(s) 82, 411
BsaI GGTCTC 1 cut(s) 1546
BsaJI CCNNGG 9 cut(s) 62, 165, 321, 438, 913, 1086, 1378, 1890, 1937
BsaWI WCCGGW 2 cut(s) 1182, 1767
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 6 cut(s) 34, 63, 68, 440, 563, 838
Bse118I RCCGGY 4 cut(s) 68, 160, 413, 433
Bse1I ACTGG 4 cut(s) 768, 1132, 1343, 1417
Bse8I GATNNNNATC 1 cut(s) 1929
BseBI CCWGG 1 cut(s) 1676
BseCI ATCGAT 2 cut(s) 676, 1167
BseDI CCNNGG 9 cut(s) 62, 165, 321, 438, 913, 1086, 1378, 1890, 1937
BseGI GGATG 6 cut(s) 766, 890, 1559, 1696, 1900, 1933
BseJI GATNNNNATC 1 cut(s) 1929
BseLI CCNNNNNNNGG 6 cut(s) 34, 63, 68, 440, 563, 838
BseNI ACTGG 4 cut(s) 768, 1132, 1343, 1417
BseRI GAGGAG 2 cut(s) 647, 893
BseX3I CGGCCG 1 cut(s) 162
BseXI GCAGC 9 cut(s) 208, 211, 214, 399, 629, 793, 1042, 1342, 1671
BseYI CCCAGC 1 cut(s) 1389
BsgI GTGCAG 2 cut(s) 590, 1319
Bsh1236I CGCG 2 cut(s) 30, 32
Bsh1285I CGRYCG 1 cut(s) 165
BshFI GGCC 6 cut(s) 9, 68, 164, 320, 437, 767
BshNI GGYRCC 2 cut(s) 81, 410
BshVI ATCGAT 2 cut(s) 676, 1167
BsiEI CGRYCG 1 cut(s) 165
BsiHKAI GWGCWC 3 cut(s) 26, 1195, 1296
BsiHKCI CYCGRG 1 cut(s) 649
BsiSI CCGG 9 cut(s) 69, 161, 300, 408, 414, 434, 1183, 1716, 1768
BslFI GGGAC 1 cut(s) 1134
BslI CCNNNNNNNGG 6 cut(s) 34, 63, 68, 440, 563, 838
BsmAI GTCTC 3 cut(s) 1415, 1460, 1546
BsmBI CGTCTC 1 cut(s) 1415
BsmFI GGGAC 1 cut(s) 1134
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 6 cut(s) 9, 68, 164, 320, 437, 767
Bso31I GGTCTC 1 cut(s) 1546
BsoBI CYCGRG 1 cut(s) 649
Bsp1286I GDGCHC 3 cut(s) 26, 1195, 1296
Bsp143I GATC 7 cut(s) 256, 282, 338, 685, 1372, 1632, 1924
Bsp19I CCATGG 1 cut(s) 1378
BspANI GGCC 6 cut(s) 9, 68, 164, 320, 437, 767
BspDI ATCGAT 2 cut(s) 676, 1167
BspFNI CGCG 2 cut(s) 30, 32
BspHI TCATGA 1 cut(s) 921
BspLI GGNNCC 7 cut(s) 83, 153, 284, 412, 1122, 1713, 1854
BspMAI CTGCAG 1 cut(s) 1689
BspPI GGATC 5 cut(s) 251, 277, 290, 346, 680
BspQI GCTCTTC 1 cut(s) 1284
BspT107I GGYRCC 2 cut(s) 81, 410
BspTNI GGTCTC 1 cut(s) 1546
BsrFI RCCGGY 4 cut(s) 68, 160, 413, 433
BsrI ACTGG 4 cut(s) 768, 1132, 1343, 1417
BssAI RCCGGY 4 cut(s) 68, 160, 413, 433
BssECI CCNNGG 9 cut(s) 62, 165, 321, 438, 913, 1086, 1378, 1890, 1937
BssMI GATC 7 cut(s) 256, 282, 338, 685, 1372, 1632, 1924
BssNI GRCGYC 2 cut(s) 82, 411
BssT1I CCWWGG 7 cut(s) 62, 321, 438, 913, 1378, 1890, 1937
Bst2UI CCWGG 1 cut(s) 1676
Bst4CI ACNGT 4 cut(s) 227, 484, 1087, 1430
Bst6I CTCTTC 3 cut(s) 993, 1284, 1849
BstACI GRCGYC 2 cut(s) 82, 411
BstBAI YACGTR 1 cut(s) 344
BstC8I GCNNGC 6 cut(s) 70, 162, 207, 435, 1232, 1557
BstDSI CCRYGG 3 cut(s) 165, 1086, 1378
BstEII GGTNACC 1 cut(s) 1581
BstENI CCTNNNNNAGG 1 cut(s) 561
BstF5I GGATG 6 cut(s) 766, 890, 1559, 1696, 1900, 1933
BstFNI CGCG 2 cut(s) 30, 32
BstH2I RGCGCY 2 cut(s) 85, 414
BstHHI GCGC 3 cut(s) 32, 84, 413
BstKTI GATC 7 cut(s) 259, 285, 341, 688, 1375, 1635, 1927
BstMAI GTCTC 3 cut(s) 1415, 1460, 1546
BstMBI GATC 7 cut(s) 256, 282, 338, 685, 1372, 1632, 1924
BstMCI CGRYCG 1 cut(s) 165
BstMWI GCNNNNNNNGC 9 cut(s) 41, 50, 134, 193, 202, 376, 434, 461, 1364
BstNI CCWGG 1 cut(s) 1676
BstNSI RCATGY 1 cut(s) 1055
BstPAI GACNNNNGTC 1 cut(s) 797
BstPI GGTNACC 1 cut(s) 1581
BstSCI CCNGG 2 cut(s) 1674, 1714
BstSFI CTRYAG 1 cut(s) 1685
BstUI CGCG 2 cut(s) 30, 32
BstV1I GCAGC 9 cut(s) 208, 211, 214, 399, 629, 793, 1042, 1342, 1671
BstV2I GAAGAC 1 cut(s) 1711
BstX2I RGATCY 2 cut(s) 282, 685
BstXI CCANNNNNNTGG 4 cut(s) 148, 994, 1385, 1570
BstYI RGATCY 2 cut(s) 282, 685
BstZI CGGCCG 1 cut(s) 162
Bsu15I ATCGAT 2 cut(s) 676, 1167
BsuI GTATCC 1 cut(s) 592
BsuRI GGCC 6 cut(s) 9, 68, 164, 320, 437, 767
BsuTUI ATCGAT 2 cut(s) 676, 1167
BtgI CCRYGG 3 cut(s) 165, 1086, 1378
BtsCI GGATG 6 cut(s) 766, 890, 1559, 1696, 1900, 1933
BtsIMutI CAGTG 5 cut(s) 141, 480, 698, 894, 1336
Cac8I GCNNGC 6 cut(s) 70, 162, 207, 435, 1232, 1557
CaiI CAGNNNCTG 1 cut(s) 787
CciI TCATGA 1 cut(s) 921
CfoI GCGC 3 cut(s) 32, 84, 413
Cfr10I RCCGGY 4 cut(s) 68, 160, 413, 433
Cfr13I GGNCC 6 cut(s) 66, 123, 227, 841, 984, 1121
ClaI ATCGAT 2 cut(s) 676, 1167
Csp6I GTAC 2 cut(s) 1410, 1508
CviAII CATG 8 cut(s) 391, 824, 833, 922, 1052, 1379, 1478, 1657
CviQI GTAC 2 cut(s) 1410, 1508
DinI GGCGCC 2 cut(s) 83, 412
DpnI GATC 7 cut(s) 258, 284, 340, 687, 1374, 1634, 1926
DpnII GATC 7 cut(s) 256, 282, 338, 685, 1372, 1632, 1924
DraI TTTAAA 1 cut(s) 966
EaeI YGGCCR 2 cut(s) 162, 435
EagI CGGCCG 1 cut(s) 162
Eam1104I CTCTTC 3 cut(s) 993, 1284, 1849
EarI CTCTTC 3 cut(s) 993, 1284, 1849
EciI GGCGGA 3 cut(s) 40, 144, 1185
Ecl136II GAGCTC 2 cut(s) 24, 1294
EclXI CGGCCG 1 cut(s) 162
Eco130I CCWWGG 7 cut(s) 62, 321, 438, 913, 1378, 1890, 1937
Eco147I AGGCCT 1 cut(s) 9
Eco24I GRGCYC 2 cut(s) 26, 1296
Eco31I GGTCTC 1 cut(s) 1546
Eco47I GGWCC 5 cut(s) 123, 227, 841, 984, 1121
Eco52I CGGCCG 1 cut(s) 162
Eco53kI GAGCTC 2 cut(s) 24, 1294
Eco57I CTGAAG 1 cut(s) 776
Eco88I CYCGRG 1 cut(s) 649
Eco91I GGTNACC 1 cut(s) 1581
EcoICRI GAGCTC 2 cut(s) 24, 1294
EcoNI CCTNNNNNAGG 1 cut(s) 561
EcoO109I RGGNCCY 1 cut(s) 1121
EcoO65I GGTNACC 1 cut(s) 1581
EcoRI GAATTC 1 cut(s) 403
EcoRII CCWGG 1 cut(s) 1674
EcoT14I CCWWGG 7 cut(s) 62, 321, 438, 913, 1378, 1890, 1937
EcoT22I ATGCAT 1 cut(s) 838
EcoT38I GRGCYC 2 cut(s) 26, 1296
EgeI GGCGCC 2 cut(s) 83, 412
EheI GGCGCC 2 cut(s) 83, 412
ErhI CCWWGG 7 cut(s) 62, 321, 438, 913, 1378, 1890, 1937
Esp3I CGTCTC 1 cut(s) 1415
FaeI CATG 8 cut(s) 394, 827, 836, 925, 1055, 1382, 1481, 1660
FaqI GGGAC 1 cut(s) 1134
FatI CATG 8 cut(s) 390, 823, 832, 921, 1051, 1378, 1477, 1656
FauI CCCGC 1 cut(s) 35
FokI GGATG 6 cut(s) 773, 877, 1546, 1703, 1907, 1920
FriOI GRGCYC 2 cut(s) 26, 1296
FspBI CTAG 6 cut(s) 210, 797, 953, 1043, 1517, 1755
GlaI GCGC 3 cut(s) 31, 83, 412
GsaI CCCAGC 1 cut(s) 1393
HaeII RGCGCY 2 cut(s) 85, 414
HaeIII GGCC 6 cut(s) 9, 68, 164, 320, 437, 767
HapII CCGG 9 cut(s) 69, 161, 300, 408, 414, 434, 1183, 1716, 1768
HhaI GCGC 3 cut(s) 32, 84, 413
Hin1I GRCGYC 2 cut(s) 82, 411
Hin1II CATG 8 cut(s) 394, 827, 836, 925, 1055, 1382, 1481, 1660
Hin6I GCGC 3 cut(s) 30, 82, 411
HinP1I GCGC 3 cut(s) 30, 82, 411
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 1013
HinfI GANTC 3 cut(s) 535, 918, 1456
HpaII CCGG 9 cut(s) 69, 161, 300, 408, 414, 434, 1183, 1716, 1768
HphI GGTGA 5 cut(s) 1015, 1367, 1409, 1465, 1575
Hpy166II GTNNAC 6 cut(s) 40, 1030, 1433, 1508, 1596, 1912
Hpy188I TCNGA 9 cut(s) 331, 540, 562, 747, 756, 931, 1080, 1420, 1461
Hpy188III TCNNGA 4 cut(s) 469, 922, 1043, 1733
Hpy8I GTNNAC 6 cut(s) 40, 1030, 1433, 1508, 1596, 1912
Hpy99I CGWCG 2 cut(s) 95, 227
HpyAV CCTTC 4 cut(s) 239, 557, 567, 751
HpyCH4III ACNGT 4 cut(s) 227, 484, 1087, 1430
HpyCH4IV ACGT 2 cut(s) 343, 865
HpyF10VI GCNNNNNNNGC 9 cut(s) 41, 50, 134, 193, 202, 376, 434, 461, 1364
HpySE526I ACGT 2 cut(s) 343, 865
Hsp92I GRCGYC 2 cut(s) 82, 411
Hsp92II CATG 8 cut(s) 394, 827, 836, 925, 1055, 1382, 1481, 1660
HspAI GCGC 3 cut(s) 30, 82, 411
KasI GGCGCC 2 cut(s) 81, 410
KroI GCCGGC 3 cut(s) 68, 160, 433
KroNI GCCGGC 3 cut(s) 70, 162, 435
Kzo9I GATC 7 cut(s) 256, 282, 338, 685, 1372, 1632, 1924
LguI GCTCTTC 1 cut(s) 1284
LmnI GCTCC 9 cut(s) 29, 157, 251, 384, 1094, 1198, 1265, 1444, 1770
Lsp1109I GCAGC 9 cut(s) 208, 211, 214, 399, 629, 793, 1042, 1342, 1671
LweI GCATC 3 cut(s) 760, 1568, 1671
MaeI CTAG 6 cut(s) 210, 797, 953, 1043, 1517, 1755
MaeII ACGT 2 cut(s) 343, 865
MaeIII GTNAC 5 cut(s) 494, 975, 1581, 1787, 1824
MalI GATC 7 cut(s) 258, 284, 340, 687, 1374, 1634, 1926
MboI GATC 7 cut(s) 256, 282, 338, 685, 1372, 1632, 1924
MboII GAAGA 6 cut(s) 1010, 1151, 1301, 1716, 1820, 1836
MflI RGATCY 2 cut(s) 282, 685
MhlI GDGCHC 3 cut(s) 26, 1195, 1296
MluCI AATT 7 cut(s) 397, 403, 456, 815, 1265, 1719, 1820
Mly113I GGCGCC 2 cut(s) 82, 411
MmeI TCCRAC 1 cut(s) 1226
Mph1103I ATGCAT 1 cut(s) 838
MroNI GCCGGC 3 cut(s) 68, 160, 433
MroXI GAANNNNTTC 2 cut(s) 738, 1026
MseI TTAA 6 cut(s) 489, 638, 957, 965, 1284, 1590
MslI CAYNNNNRTG 2 cut(s) 146, 1568
MspA1I CMGCKG 1 cut(s) 620
MspI CCGG 9 cut(s) 69, 161, 300, 408, 414, 434, 1183, 1716, 1768
MspR9I CCNGG 2 cut(s) 1676, 1716
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 1 cut(s) 1676
MvnI CGCG 2 cut(s) 30, 32
MwoI GCNNNNNNNGC 9 cut(s) 41, 50, 134, 193, 202, 376, 434, 461, 1364
NaeI GCCGGC 3 cut(s) 70, 162, 435
NarI GGCGCC 2 cut(s) 82, 411
NciI CCSGG 1 cut(s) 1716
NcoI CCATGG 1 cut(s) 1378
NdeII GATC 7 cut(s) 256, 282, 338, 685, 1372, 1632, 1924
NgoMIV GCCGGC 3 cut(s) 68, 160, 433
NlaIII CATG 8 cut(s) 394, 827, 836, 925, 1055, 1382, 1481, 1660
NlaIV GGNNCC 7 cut(s) 83, 153, 284, 412, 1122, 1713, 1854
NmuCI GTSAC 2 cut(s) 975, 1581
NsiI ATGCAT 1 cut(s) 838
NspI RCATGY 1 cut(s) 1055
OliI CACNNNNGTG 2 cut(s) 146, 1568
PagI TCATGA 1 cut(s) 921
PceI AGGCCT 1 cut(s) 9
PciSI GCTCTTC 1 cut(s) 1284
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PctI GAATGC 1 cut(s) 464
PdiI GCCGGC 3 cut(s) 70, 162, 435
PdmI GAANNNNTTC 2 cut(s) 738, 1026
PfeI GAWTC 3 cut(s) 535, 918, 1456
PflMI CCANNNNNTGG 2 cut(s) 63, 838
PfoI TCCNGGA 1 cut(s) 1714
PluTI GGCGCC 2 cut(s) 85, 414
Ppu21I YACGTR 1 cut(s) 344
PpuMI RGGWCCY 1 cut(s) 1121
PshAI GACNNNNGTC 1 cut(s) 797
PsiI TTATAA 1 cut(s) 294
Psp124BI GAGCTC 2 cut(s) 26, 1296
Psp5II RGGWCCY 1 cut(s) 1121
Psp6I CCWGG 1 cut(s) 1674
PspEI GGTNACC 1 cut(s) 1581
PspFI CCCAGC 1 cut(s) 1389
PspGI CCWGG 1 cut(s) 1674
PspN4I GGNNCC 7 cut(s) 83, 153, 284, 412, 1122, 1713, 1854
PspPI GGNCC 6 cut(s) 66, 123, 227, 841, 984, 1121
PspPPI RGGWCCY 1 cut(s) 1121
PstI CTGCAG 1 cut(s) 1689
PstNI CAGNNNCTG 1 cut(s) 787
PsuI RGATCY 2 cut(s) 282, 685
PvuII CAGCTG 1 cut(s) 620
RsaI GTAC 2 cut(s) 1411, 1509
RsaNI GTAC 2 cut(s) 1410, 1508
RseI CAYNNNNRTG 2 cut(s) 146, 1568
SacI GAGCTC 2 cut(s) 26, 1296
SapI GCTCTTC 1 cut(s) 1284
SaqAI TTAA 6 cut(s) 489, 638, 957, 965, 1284, 1590
Sau3AI GATC 7 cut(s) 256, 282, 338, 685, 1372, 1632, 1924
Sau96I GGNCC 6 cut(s) 66, 123, 227, 841, 984, 1121
ScaI AGTACT 1 cut(s) 1411
ScrFI CCNGG 2 cut(s) 1676, 1716
SduI GDGCHC 3 cut(s) 26, 1195, 1296
SfaNI GCATC 3 cut(s) 760, 1568, 1671
SfcI CTRYAG 1 cut(s) 1685
SfoI GGCGCC 2 cut(s) 83, 412
SinI GGWCC 5 cut(s) 123, 227, 841, 984, 1121
SmiMI CAYNNNNRTG 2 cut(s) 146, 1568
SpeI ACTAGT 3 cut(s) 796, 952, 1516
Sse9I AATT 7 cut(s) 397, 403, 456, 815, 1265, 1719, 1820
SseBI AGGCCT 1 cut(s) 9
SspDI GGCGCC 2 cut(s) 81, 410
SspMI CTAG 6 cut(s) 210, 797, 953, 1043, 1517, 1755
SstI GAGCTC 2 cut(s) 26, 1296
StuI AGGCCT 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 1674, 1714
StyI CCWWGG 7 cut(s) 62, 321, 438, 913, 1378, 1890, 1937
TaaI ACNGT 4 cut(s) 227, 484, 1087, 1430
TaiI ACGT 2 cut(s) 346, 868
TaqI TCGA 6 cut(s) 93, 468, 518, 676, 876, 1167
TaqII GACCGA 1 cut(s) 858
TasI AATT 7 cut(s) 397, 403, 456, 815, 1265, 1719, 1820
TatI WGTACW 2 cut(s) 1409, 1507
TauI GCSGC 6 cut(s) 35, 56, 137, 140, 160, 1237
TfiI GAWTC 3 cut(s) 535, 918, 1456
Tru1I TTAA 6 cut(s) 489, 638, 957, 965, 1284, 1590
Tru9I TTAA 6 cut(s) 489, 638, 957, 965, 1284, 1590
TscAI CASTG 5 cut(s) 148, 487, 698, 901, 1343
TseFI GTSAC 2 cut(s) 975, 1581
TseI GCWGC 9 cut(s) 196, 199, 202, 387, 617, 781, 1055, 1355, 1684
Tsp45I GTSAC 2 cut(s) 975, 1581
TspDTI ATGAA 4 cut(s) 258, 876, 879, 1520
TspGWI ACGGA 1 cut(s) 608
TspRI CASTG 5 cut(s) 148, 487, 698, 901, 1343
Van91I CCANNNNNTGG 2 cut(s) 63, 838
VpaK11BI GGWCC 5 cut(s) 123, 227, 841, 984, 1121
XagI CCTNNNNNAGG 1 cut(s) 561
XapI RAATTY 2 cut(s) 397, 403
XbaI TCTAGA 1 cut(s) 1042
XceI RCATGY 1 cut(s) 1055
XmnI GAANNNNTTC 2 cut(s) 738, 1026
XspI CTAG 6 cut(s) 210, 797, 953, 1043, 1517, 1755
ZrmI AGTACT 1 cut(s) 1411
Zsp2I ATGCAT 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.