FvH4_1g26650

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012763
Physical Location & Seq
Reverse (-)
18477277 .. 18479676
2400 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g26650.t1

Sequence Viewer

Length: 1416 bp
ATGAAGAAGCTTTCCAAATTGTCACTAGTGTGCGTTATATTCTTTGGCTTTTTGCTTCTGTCAACCCAAGCCAGATGGCATAACCATACCAAACACAGCAAACACAACCACCCTCACAAGATTTCTTACATTTCACAGCCTCCTTCCTCTTCACCTGGACCTGCTGCTAGCTACAGTCCTCCTCCTGAAGAGCCTAAGAATCCATACAACTCCACACCTGGTGTTTTCGATGTTCGATTATATGGGGCTGTGGGGGATGGCCAAACAGATGACACTCAAGCCTTTAAAATGGCTTGGGACACAGCCTGCCAAAGTGAATCACCAGCAAAGATACTTGTTCCGAACCATTTCACATTCATGATTCAGTCCACAATATTTACGGGTCCCTGCCTTTCAGGCCTGGTGTTACAGGTCGATGGGGTTATAGTACCACCTGATGGACCAGAGTCCTGGCCAAAAAACAACAGCAGGCGCCAATGGCTCGTTTTTTATCGAATCAACAAGATGTCGCTGCAAGGGAGTGGTGTTATAGATGGGAGAGGAGACAAATGGTGGCAACTTCCCTGCAAGCCCCACATGGGAATAAATGGAACCACCTTGCCTGGACCATGTGATAGCCCAGTTGCCATTAGATTTTTCATGAGCTCTAACTTGACAGTACAAGGTCTTAGGATTAAGAATAGCCCCCAATTCCACTTCAGATTTGATGGTTGCATAAATGTGAATGTAGACTCCATTTCTATATCAGCTCCTGCTCGAAGTCCGAATACAGATGGGATTCACATAGAGAACACCAATGATGTAAAAATTTATAATTCTGTAATTTCTAATGGTGATGATTGTGTATCAATTGGATCAGGCTGTTATGATGTAGACATTAGAAACATCACTTGTGGCCCTGGTCATGGAATCAGCATTGGGAGTCTCGGAAACCACAACTCGCGAGCCTGTGTTAAAAATATTACAGTTCGAGACTCTATTATTAGAGTTTCGGATAATGGAGTTAGGATCAAGACATGGCAGGGTGGATCCGGAGCAGTGTCCGGAGTAACTTTCAGTAACATTCACATGGACAATGTTAGGAACCCCATTATGATTGATCAATTTTACTGCCTTACTAAGCAGTGCAGCAATCAAACCGCAGCGGTATATGTATCGAACATCCTATACTCGGGCATAAAAGGAACCTATGATGTCCGGAATGCTCCAATGCATTTTGGCTGCAGTGATTCTATGCCATGCACCAACTTAACACTCTCAGATGTTGAGCTTCTTCCAGCTGTAGGAGATATGATGTCAGACCCTTATTGTTGGAATGCTTACGGACAACTGCAAACGCTTACTATTCCACCAGTCTCTTGCTTGTTGGAGGGTGCTCCTAGAACTTTACTGAGCGATGAAATAGACCGCTGCTGA

Protein Analysis

472

Amino Acids

51.49

Weight (kDa)

7.52

Isoelectric Point (pI)

53.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 103 - 425 3.9e-90 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012763)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02460
fragaria_vesca FvH4_1g26650
malus_domestica MD09G1287300.v1.1 MD17G1279500.v1.1
prunus_persica Prupe.3G005900_v2.0.a1 Prupe.6G307200_v2.0.a1
pyrus_communis pycom09g19460 pycom17g27760
rosa_chinensis RchiOBHm_Chr3g0486841
rosa_laevigata RLG00000023055
rosa_multiflora Rmu_sc0005709.1_g000005
rosa_roxburghii Rroxscaffold_6G00395520
rosa_rugosa Rorug03G0227400.1
rosa_samantha Rh3AG277500 Rh3BG312600 Rh3CG311500 Rh3DG308100
rosa_wichuraiana Rw3G024640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 813
Acc36I ACCTGC 1 cut(s) 169
AccB1I GGYRCC 1 cut(s) 471
AccB7I CCANNNNNTGG 1 cut(s) 437
AccI GTMKAC 2 cut(s) 729, 873
AccII CGCG 1 cut(s) 943
AccIII TCCGGA 3 cut(s) 1031, 1043, 1197
AciI CCGC 3 cut(s) 1140, 1145, 1408
AclWI GGATC 4 cut(s) 862, 1016, 1023, 1036
AcoI YGGCCR 2 cut(s) 259, 452
AcsI RAATTY 1 cut(s) 807
AcuI CTGAAG 2 cut(s) 207, 682
AcyI GRCGYC 1 cut(s) 472
AdeI CACNNNGTG 1 cut(s) 221
AfaI GTAC 2 cut(s) 429, 660
AfiI CCNNNNNNNGG 5 cut(s) 437, 578, 905, 1171, 1283
AhlI ACTAGT 1 cut(s) 25
AjnI CCWGG 6 cut(s) 154, 217, 399, 449, 601, 898
AleI CACNNNNGTG 1 cut(s) 28
AluBI AGCT 6 cut(s) 10, 171, 645, 749, 1270, 1280
AluI AGCT 6 cut(s) 10, 171, 645, 749, 1270, 1280
Alw21I GWGCWC 2 cut(s) 647, 1378
Alw26I GTCTC 4 cut(s) 537, 929, 966, 1360
AlwI GGATC 4 cut(s) 862, 1016, 1023, 1036
AlwNI CAGNNNCTG 1 cut(s) 752
Ama87I CYCGRG 1 cut(s) 1171
Aor13HI TCCGGA 3 cut(s) 1031, 1043, 1197
AoxI GGCC 4 cut(s) 259, 397, 452, 895
ApeKI GCWGC 6 cut(s) 164, 511, 1128, 1142, 1221, 1410
ApoI RAATTY 1 cut(s) 807
Asp700I GAANNNNTTC 1 cut(s) 347
AspLEI GCGC 1 cut(s) 474
AspS9I GGNCC 5 cut(s) 158, 383, 440, 605, 896
AsuHPI GGTGA 3 cut(s) 144, 312, 845
AsuNHI GCTAGC 1 cut(s) 167
AvaI CYCGRG 1 cut(s) 1171
AvaII GGWCC 4 cut(s) 158, 383, 440, 605
BalI TGGCCA 2 cut(s) 261, 454
BamHI GGATCC 1 cut(s) 1028
BanI GGYRCC 1 cut(s) 471
BanII GRGCYC 1 cut(s) 647
BarI GAAGNNNNNNTAC 2 cut(s) 751, 783
Bbv12I GWGCWC 2 cut(s) 647, 1378
BbvI GCAGC 6 cut(s) 151, 498, 1140, 1154, 1208, 1397
BccI CCATC 7 cut(s) 69, 251, 410, 431, 527, 701, 767
BciT130I CCWGG 6 cut(s) 156, 219, 401, 451, 603, 900
BclI TGATCA 1 cut(s) 1099
BcoDI GTCTC 4 cut(s) 537, 929, 966, 1360
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 3 cut(s) 26, 168, 1380
BfmI CTRYAG 3 cut(s) 172, 1222, 1281
BfoI RGCGCY 1 cut(s) 475
BfuAI ACCTGC 1 cut(s) 169
BglI GCCNNNNNGGC 1 cut(s) 396
BisI GCNGC 6 cut(s) 165, 512, 1129, 1143, 1222, 1411
BlsI GCNGC 6 cut(s) 166, 513, 1130, 1144, 1223, 1412
Bme1390I CCNGG 6 cut(s) 156, 219, 401, 451, 603, 900
Bme18I GGWCC 4 cut(s) 158, 383, 440, 605
BmeT110I CYCGRG 1 cut(s) 1171
BmgT120I GGNCC 5 cut(s) 158, 383, 440, 605, 896
BmiI GGNNCC 7 cut(s) 384, 385, 473, 592, 1030, 1085, 1186
BmrFI CCNGG 6 cut(s) 156, 219, 401, 451, 603, 900
BmrI ACTGGG 1 cut(s) 614
BmtI GCTAGC 1 cut(s) 171
BmuI ACTGGG 1 cut(s) 614
BoxI GACNNNNGTC 1 cut(s) 445
BpuEI CTTGAG 1 cut(s) 261
BsaHI GRCGYC 1 cut(s) 472
BsaJI CCNNGG 1 cut(s) 898
BsaWI WCCGGW 3 cut(s) 1031, 1043, 1197
Bsc4I CCNNNNNNNGG 5 cut(s) 437, 578, 905, 1171, 1283
Bse1I ACTGG 2 cut(s) 620, 1352
BseAI TCCGGA 3 cut(s) 1031, 1043, 1197
BseBI CCWGG 6 cut(s) 156, 219, 401, 451, 603, 900
BseDI CCNNGG 1 cut(s) 898
BseGI GGATG 2 cut(s) 262, 1161
BseLI CCNNNNNNNGG 5 cut(s) 437, 578, 905, 1171, 1283
BseMII CTCAG 2 cut(s) 1272, 1382
BseNI ACTGG 2 cut(s) 620, 1352
BseRI GAGGAG 2 cut(s) 171, 555
BseXI GCAGC 6 cut(s) 151, 498, 1140, 1154, 1208, 1397
BsgI GTGCAG 1 cut(s) 1147
Bsh1236I CGCG 1 cut(s) 943
BshFI GGCC 4 cut(s) 261, 399, 454, 897
BshNI GGYRCC 1 cut(s) 471
BsiHKAI GWGCWC 2 cut(s) 647, 1378
BsiHKCI CYCGRG 1 cut(s) 1171
BsiSI CCGG 3 cut(s) 1032, 1044, 1198
BslFI GGGAC 2 cut(s) 311, 369
BslI CCNNNNNNNGG 5 cut(s) 437, 578, 905, 1171, 1283
BsmAI GTCTC 4 cut(s) 537, 929, 966, 1360
BsmFI GGGAC 2 cut(s) 311, 369
BsmI GAATGC 2 cut(s) 1207, 1321
BsnI GGCC 4 cut(s) 261, 399, 454, 897
BsoBI CYCGRG 1 cut(s) 1171
Bsp1286I GDGCHC 2 cut(s) 647, 1378
Bsp13I TCCGGA 3 cut(s) 1031, 1043, 1197
Bsp143I GATC 4 cut(s) 854, 1008, 1028, 1099
Bsp68I TCGCGA 1 cut(s) 943
BspACI CCGC 3 cut(s) 1140, 1145, 1408
BspANI GGCC 4 cut(s) 261, 399, 454, 897
BspCNI CTCAG 2 cut(s) 1271, 1383
BspEI TCCGGA 3 cut(s) 1031, 1043, 1197
BspFNI CGCG 1 cut(s) 943
BspHI TCATGA 2 cut(s) 357, 639
BspLI GGNNCC 7 cut(s) 384, 385, 473, 592, 1030, 1085, 1186
BspMAI CTGCAG 1 cut(s) 1226
BspMI ACCTGC 1 cut(s) 169
BspOI GCTAGC 1 cut(s) 171
BspPI GGATC 4 cut(s) 862, 1016, 1023, 1036
BspQI GCTCTTC 1 cut(s) 183
BspT107I GGYRCC 1 cut(s) 471
BsrI ACTGG 2 cut(s) 620, 1352
BssECI CCNNGG 1 cut(s) 898
BssMI GATC 4 cut(s) 854, 1008, 1028, 1099
BssNI GRCGYC 1 cut(s) 472
Bst2UI CCWGG 6 cut(s) 156, 219, 401, 451, 603, 900
Bst4CI ACNGT 3 cut(s) 176, 658, 967
Bst6I CTCTTC 2 cut(s) 154, 183
BstACI GRCGYC 1 cut(s) 472
BstC8I GCNNGC 5 cut(s) 169, 307, 470, 569, 945
BstDEI CTNAG 5 cut(s) 195, 668, 1119, 1258, 1391
BstF5I GGATG 2 cut(s) 262, 1161
BstFNI CGCG 1 cut(s) 943
BstH2I RGCGCY 1 cut(s) 475
BstHHI GCGC 1 cut(s) 474
BstKTI GATC 4 cut(s) 857, 1011, 1031, 1102
BstMAI GTCTC 4 cut(s) 537, 929, 966, 1360
BstMBI GATC 4 cut(s) 854, 1008, 1028, 1099
BstMWI GCNNNNNNNGC 2 cut(s) 396, 478
BstNI CCWGG 6 cut(s) 156, 219, 401, 451, 603, 900
BstPAI GACNNNNGTC 1 cut(s) 445
BstSCI CCNGG 6 cut(s) 154, 217, 399, 449, 601, 898
BstSFI CTRYAG 3 cut(s) 172, 1222, 1281
BstUI CGCG 1 cut(s) 943
BstV1I GCAGC 6 cut(s) 151, 498, 1140, 1154, 1208, 1397
BstX2I RGATCY 1 cut(s) 1028
BstXI CCANNNNNNTGG 1 cut(s) 450
BstYI RGATCY 1 cut(s) 1028
BsuRI GGCC 4 cut(s) 261, 399, 454, 897
BtgZI GCGATG 1 cut(s) 1410
BtsCI GGATG 2 cut(s) 262, 1161
BtsI GCAGTG 3 cut(s) 1044, 1130, 1231
BtsIMutI CAGTG 3 cut(s) 1044, 1130, 1231
BtuMI TCGCGA 1 cut(s) 943
BveI ACCTGC 1 cut(s) 169
Cac8I GCNNGC 5 cut(s) 169, 307, 470, 569, 945
CaiI CAGNNNCTG 1 cut(s) 752
CciI TCATGA 2 cut(s) 357, 639
CfoI GCGC 1 cut(s) 474
Cfr13I GGNCC 5 cut(s) 158, 383, 440, 605, 896
CsiI ACCWGGT 1 cut(s) 217
Csp6I GTAC 2 cut(s) 428, 659
CviAII CATG 8 cut(s) 358, 577, 609, 640, 905, 1017, 1069, 1239
CviQI GTAC 2 cut(s) 428, 659
DdeI CTNAG 5 cut(s) 195, 668, 1119, 1258, 1391
DinI GGCGCC 1 cut(s) 473
DpnI GATC 4 cut(s) 856, 1010, 1030, 1101
DpnII GATC 4 cut(s) 854, 1008, 1028, 1099
DraI TTTAAA 1 cut(s) 286
DraIII CACNNNGTG 1 cut(s) 221
EaeI YGGCCR 2 cut(s) 259, 452
Eam1104I CTCTTC 2 cut(s) 154, 183
EarI CTCTTC 2 cut(s) 154, 183
Ecl136II GAGCTC 1 cut(s) 645
Eco147I AGGCCT 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 647
Eco47I GGWCC 4 cut(s) 158, 383, 440, 605
Eco53kI GAGCTC 1 cut(s) 645
Eco57I CTGAAG 2 cut(s) 207, 682
Eco88I CYCGRG 1 cut(s) 1171
EcoICRI GAGCTC 1 cut(s) 645
EcoO109I RGGNCCY 1 cut(s) 383
EcoRII CCWGG 6 cut(s) 154, 217, 399, 449, 601, 898
EcoT22I ATGCAT 1 cut(s) 1215
EcoT38I GRGCYC 1 cut(s) 647
EgeI GGCGCC 1 cut(s) 473
EheI GGCGCC 1 cut(s) 473
FaeI CATG 8 cut(s) 361, 580, 612, 643, 908, 1020, 1072, 1242
FaqI GGGAC 2 cut(s) 311, 369
FatI CATG 8 cut(s) 357, 576, 608, 639, 904, 1016, 1068, 1238
FbaI TGATCA 1 cut(s) 1099
FblI GTMKAC 2 cut(s) 729, 873
Fnu4HI GCNGC 6 cut(s) 165, 512, 1129, 1143, 1222, 1411
FokI GGATG 2 cut(s) 269, 1148
FriOI GRGCYC 1 cut(s) 647
Fsp4HI GCNGC 6 cut(s) 165, 512, 1129, 1143, 1222, 1411
FspBI CTAG 3 cut(s) 26, 168, 1380
GlaI GCGC 1 cut(s) 473
GluI GCNGC 6 cut(s) 165, 512, 1129, 1143, 1222, 1411
HaeII RGCGCY 1 cut(s) 475
HaeIII GGCC 4 cut(s) 261, 399, 454, 897
HapII CCGG 3 cut(s) 1032, 1044, 1198
HhaI GCGC 1 cut(s) 474
Hin1I GRCGYC 1 cut(s) 472
Hin1II CATG 8 cut(s) 361, 580, 612, 643, 908, 1020, 1072, 1242
Hin6I GCGC 1 cut(s) 472
HinP1I GCGC 1 cut(s) 472
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HindIII AAGCTT 1 cut(s) 8
HpaII CCGG 3 cut(s) 1032, 1044, 1198
HphI GGTGA 3 cut(s) 144, 312, 845
Hpy166II GTNNAC 4 cut(s) 63, 369, 730, 874
Hpy188I TCNGA 7 cut(s) 342, 701, 765, 929, 994, 1261, 1300
Hpy188III TCNNGA 9 cut(s) 185, 358, 640, 942, 971, 1012, 1032, 1044, 1198
Hpy8I GTNNAC 4 cut(s) 63, 369, 730, 874
HpyAV CCTTC 1 cut(s) 153
HpyCH4III ACNGT 3 cut(s) 176, 658, 967
HpyCH4V TGCA 8 cut(s) 514, 567, 714, 1128, 1213, 1224, 1242, 1333
HpyF10VI GCNNNNNNNGC 2 cut(s) 396, 478
HpyF3I CTNAG 5 cut(s) 195, 668, 1119, 1258, 1391
Hsp92I GRCGYC 1 cut(s) 472
Hsp92II CATG 8 cut(s) 361, 580, 612, 643, 908, 1020, 1072, 1242
HspAI GCGC 1 cut(s) 472
KasI GGCGCC 1 cut(s) 471
KflI GGGWCCC 1 cut(s) 383
Kpn2I TCCGGA 3 cut(s) 1031, 1043, 1197
Ksp22I TGATCA 1 cut(s) 1099
Kzo9I GATC 4 cut(s) 854, 1008, 1028, 1099
LguI GCTCTTC 1 cut(s) 183
LmnI GCTCC 4 cut(s) 754, 1034, 1210, 1381
Lsp1109I GCAGC 6 cut(s) 151, 498, 1140, 1154, 1208, 1397
MabI ACCWGGT 1 cut(s) 217
MaeI CTAG 3 cut(s) 26, 168, 1380
MaeIII GTNAC 4 cut(s) 21, 405, 1048, 1058
MalI GATC 4 cut(s) 856, 1010, 1030, 1101
MboI GATC 4 cut(s) 854, 1008, 1028, 1099
MboII GAAGA 4 cut(s) 16, 141, 200, 1265
MfeI CAATTG 1 cut(s) 849
MflI RGATCY 1 cut(s) 1028
MhlI GDGCHC 2 cut(s) 647, 1378
MlsI TGGCCA 2 cut(s) 261, 454
MluCI AATT 7 cut(s) 17, 689, 807, 814, 822, 849, 1103
MluNI TGGCCA 2 cut(s) 261, 454
Mly113I GGCGCC 1 cut(s) 472
MlyI GAGTC 4 cut(s) 455, 725, 931, 968
MmeI TCCRAC 2 cut(s) 1292, 1347
MnlI CCTC 7 cut(s) 123, 150, 157, 189, 192, 533, 1363
Mox20I TGGCCA 2 cut(s) 261, 454
Mph1103I ATGCAT 1 cut(s) 1215
MroI TCCGGA 3 cut(s) 1031, 1043, 1197
MroXI GAANNNNTTC 1 cut(s) 347
MscI TGGCCA 2 cut(s) 261, 454
MseI TTAA 4 cut(s) 285, 675, 954, 1250
MslI CAYNNNNRTG 4 cut(s) 28, 356, 719, 1067
Msp20I TGGCCA 2 cut(s) 261, 454
MspA1I CMGCKG 3 cut(s) 1145, 1280, 1410
MspI CCGG 3 cut(s) 1032, 1044, 1198
MspR9I CCNGG 6 cut(s) 156, 219, 401, 451, 603, 900
MunI CAATTG 1 cut(s) 849
Mva1269I GAATGC 2 cut(s) 1207, 1321
MvaI CCWGG 6 cut(s) 156, 219, 401, 451, 603, 900
MvnI CGCG 1 cut(s) 943
MwoI GCNNNNNNNGC 2 cut(s) 396, 478
NarI GGCGCC 1 cut(s) 472
NdeII GATC 4 cut(s) 854, 1008, 1028, 1099
NheI GCTAGC 1 cut(s) 167
NlaIII CATG 8 cut(s) 361, 580, 612, 643, 908, 1020, 1072, 1242
NlaIV GGNNCC 7 cut(s) 384, 385, 473, 592, 1030, 1085, 1186
NmuCI GTSAC 1 cut(s) 21
NruI TCGCGA 1 cut(s) 943
NsiI ATGCAT 1 cut(s) 1215
OliI CACNNNNGTG 1 cut(s) 28
PagI TCATGA 2 cut(s) 357, 639
PceI AGGCCT 1 cut(s) 399
PciSI GCTCTTC 1 cut(s) 183
PctI GAATGC 2 cut(s) 1207, 1321
PdmI GAANNNNTTC 1 cut(s) 347
PfeI GAWTC 7 cut(s) 199, 317, 361, 495, 778, 909, 1229
PflMI CCANNNNNTGG 1 cut(s) 437
PkrI GCNGC 6 cut(s) 166, 513, 1130, 1144, 1223, 1412
PleI GAGTC 4 cut(s) 454, 725, 930, 968
PluTI GGCGCC 1 cut(s) 475
PpsI GAGTC 4 cut(s) 454, 725, 930, 968
PpuMI RGGWCCY 1 cut(s) 383
PshAI GACNNNNGTC 1 cut(s) 445
PsiI TTATAA 1 cut(s) 813
Psp124BI GAGCTC 1 cut(s) 647
Psp5II RGGWCCY 1 cut(s) 383
Psp6I CCWGG 6 cut(s) 154, 217, 399, 449, 601, 898
PspGI CCWGG 6 cut(s) 154, 217, 399, 449, 601, 898
PspN4I GGNNCC 7 cut(s) 384, 385, 473, 592, 1030, 1085, 1186
PspPI GGNCC 5 cut(s) 158, 383, 440, 605, 896
PspPPI RGGWCCY 1 cut(s) 383
PsrI GAACNNNNNNTAC 2 cut(s) 1151, 1183
PstI CTGCAG 1 cut(s) 1226
PstNI CAGNNNCTG 1 cut(s) 752
PsuI RGATCY 1 cut(s) 1028
PvuII CAGCTG 1 cut(s) 1280
RruI TCGCGA 1 cut(s) 943
RsaI GTAC 2 cut(s) 429, 660
RsaNI GTAC 2 cut(s) 428, 659
RseI CAYNNNNRTG 4 cut(s) 28, 356, 719, 1067
SacI GAGCTC 1 cut(s) 647
SapI GCTCTTC 1 cut(s) 183
SaqAI TTAA 4 cut(s) 285, 675, 954, 1250
SatI GCNGC 6 cut(s) 165, 512, 1129, 1143, 1222, 1411
Sau3AI GATC 4 cut(s) 854, 1008, 1028, 1099
Sau96I GGNCC 5 cut(s) 158, 383, 440, 605, 896
SchI GAGTC 4 cut(s) 455, 725, 931, 968
ScrFI CCNGG 6 cut(s) 156, 219, 401, 451, 603, 900
SduI GDGCHC 2 cut(s) 647, 1378
SexAI ACCWGGT 1 cut(s) 217
SfcI CTRYAG 3 cut(s) 172, 1222, 1281
SfoI GGCGCC 1 cut(s) 473
SinI GGWCC 4 cut(s) 158, 383, 440, 605
SmiMI CAYNNNNRTG 4 cut(s) 28, 356, 719, 1067
SmlI CTYRAG 1 cut(s) 276
SmoI CTYRAG 1 cut(s) 276
SpeI ACTAGT 1 cut(s) 25
Sse9I AATT 7 cut(s) 17, 689, 807, 814, 822, 849, 1103
SseBI AGGCCT 1 cut(s) 399
SsiI CCGC 3 cut(s) 1140, 1145, 1408
SspDI GGCGCC 1 cut(s) 471
SspI AATATT 2 cut(s) 375, 961
SspMI CTAG 3 cut(s) 26, 168, 1380
SstI GAGCTC 1 cut(s) 647
StuI AGGCCT 1 cut(s) 399
StyD4I CCNGG 6 cut(s) 154, 217, 399, 449, 601, 898
TaaI ACNGT 3 cut(s) 176, 658, 967
TaqI TCGA 7 cut(s) 228, 235, 414, 493, 757, 970, 1157
TasI AATT 7 cut(s) 17, 689, 807, 814, 822, 849, 1103
TatI WGTACW 1 cut(s) 658
TfiI GAWTC 7 cut(s) 199, 317, 361, 495, 778, 909, 1229
Tru1I TTAA 4 cut(s) 285, 675, 954, 1250
Tru9I TTAA 4 cut(s) 285, 675, 954, 1250
TscAI CASTG 3 cut(s) 1044, 1130, 1231
TseFI GTSAC 1 cut(s) 21
TseI GCWGC 6 cut(s) 164, 511, 1128, 1142, 1221, 1410
Tsp45I GTSAC 1 cut(s) 21
TspDTI ATGAA 4 cut(s) 17, 346, 628, 1413
TspGWI ACGGA 1 cut(s) 1338
TspRI CASTG 3 cut(s) 1044, 1130, 1231
Van91I CCANNNNNTGG 1 cut(s) 437
VpaK11BI GGWCC 4 cut(s) 158, 383, 440, 605
XapI RAATTY 1 cut(s) 807
XmiI GTMKAC 2 cut(s) 729, 873
XmnI GAANNNNTTC 1 cut(s) 347
XspI CTAG 3 cut(s) 26, 168, 1380
Zsp2I ATGCAT 1 cut(s) 1215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.